CN101637167A - Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof - Google Patents
Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof Download PDFInfo
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- CN101637167A CN101637167A CN200910094880A CN200910094880A CN101637167A CN 101637167 A CN101637167 A CN 101637167A CN 200910094880 A CN200910094880 A CN 200910094880A CN 200910094880 A CN200910094880 A CN 200910094880A CN 101637167 A CN101637167 A CN 101637167A
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Abstract
The invention provides a special sterilizing and strengthening agent for sugarcane tissue culture seedlings and a use method thereof. The agent comprises carbendazim, naphthylacetic acid and filling materials based on mass ratio of 1000:1. The use method comprises the following steps: weighing a reagent according to effective ingredient percentage to prepare into 0.3% of solution, soaking the sugarcane tissue culture seedlings for 3min, and then temporarily planting the seedlings in a seedling bed. The test result shows that for the temporarily planted sugarcane tissue culture seedlings treated by the formula, survival rate of grade 1 seedlings is 77.57%; compared with the seedlings through conventional treatment, survived plants have obviously improved quality with plant height increasedby 104.56%, leaf length increased by 89.37%, leaf width increased by 53.85%, root length increased by 105.52%, and obviously increased roots. The formula and the use method are applicable to popularization and application in sugarcane scientific research institutions and seedling production enterprises, and the formula can be also applicable to new product development for reagent manufacturers.
Description
Technical field:
The present invention relates to short strong agent of a kind of sugarcane tissue-culture seedling special sterilizing and using method.
Background technology:
Sugarcane is the important sugar [yielding of China, and more than 60%, applying rapidly of Sugarcane Superior Variety becomes the direct driving force that promotes the sucrose industry development to Sugarcane Superior Variety to the contribution rate of sucrose industry.Sugarcane is the pierre crop, proliferative speed is slow, the conventional cultivation sowing quantity is big and produce upward, the mu sowing quantity is up to the 8000-10000 bud. and therefore adopt modern biotechnology to breed improved seeds fast, become the effective way of quickening the quick Transformation Application of Sugarcane Superior Variety, in actual application, it is outdoor that to heel in administrative skill most important, directly affect and heel in survival rate and survive the seedling quality, heel in survival rate for improving in the production, paying special attention to strengthen mould prevents, before heeling in the sugarcane tissue-culture seedling is soaked in 0.3% carbendazim 5min or is soaked in 0.1% potassium permanganate 1min disinfection, can effectively prevent and treat mould, solve the low problem of survival rate, but it be of poor quality to survive seedling.
Summary of the invention:
The objective of the invention is for heeling in of sugarcane tissue-culture seedling provides short strong agent of a kind of special sterilizing and using method, it can effectively solve present sugarcane tissue-culture technology and apply in the process, and it is not high that the one-level seedling is heeled in survival rate, survives the ropy problem of seedling.
The present invention is achieved through the following technical solutions:
The present invention is according to the reagent principle that is mixed, the mechanism of action according to carbendazim and naa, again according to sugarcane tissue-culture seedling characteristic, the short strong agent prescription of sugarcane tissue-culture seedling special sterilizing is scientifically proposed, the short strong agent of this sterilization comprises carbendazim, naa and inserts, carbendazim is 1000: 1 than the mass ratio of naa, during use, according to active ingredient percentage, taking by weighing that reagent joins it is 0.3% solution, with this solution the sugarcane tissue-culture seedling is carried out the 3min immersion treatment after, heel in the seedbed, get final product effective mould fungus inhibition, promote quick normal root, raising survives the plant quality.
The beneficial effect that the present invention has is as follows:
The present invention has the characteristic that promotes growth and rooting effect according to naa, creationary carbendazim solution and variable concentrations naa solution are mixed, by comparative test, filtered out desirable mixing formula, and the science of proposition using method, the one-level that is applied to the sugarcane tissue-culture seedling is heeled in operation, has reached fungus and mildew resistance and short root and has cultivated the effect of neglecting neither strong sprout.Show by experimental study, use this prescription and handle the sugarcane tissue-culture seedling, the survival rate that one-level is heeled in seedling is 77.57%, the quality that survives plant significantly improves, and plant height comparison conventional treatment has increased by 104.56%, and leaf length has increased by 89.37%, leaf is wide to have increased by 53.85%, root length has increased the bar of 105.52%. root and has counted showed increased, and this prescription and using method are fit to sugarcane R﹠D institution, seedling manufacturing enterprise promotes the use of, and also can be used as the reagent manufacturer use of developing new product.
Embodiment:
The short strong agent of sugarcane tissue-culture seedling special sterilizing provided by the invention can be produced by the chemical reagent manufacturer according to above-mentioned formula rate production, also can purchase the raw material packing that is mixed by R﹠D institution or reagent dealer.
The short strong agent using method of sugarcane tissue-culture seedling special sterilizing: carry out one-level when heeling at the sugarcane tissue-culture seedling, according to active ingredient percentage, taking by weighing that an amount of reagent joins it is 0.3% solution, with this solution the sugarcane tissue-culture seedling is carried out the 3min immersion treatment after, heel in the seedbed, can be effective.
Claims (2)
1, the short strong agent of a kind of sugarcane tissue-culture seedling special sterilizing, it is characterized in that: comprise carbendazim and naa and inserts, the mass ratio of carbendazim and naa is 1000: 1.
2, the short strong agent of sugarcane tissue-culture seedling special sterilizing according to claim 1 is characterized in that: use is, according to active ingredient percentage, it is joined is 0.3% solution, with this solution the sugarcane tissue-culture seedling is carried out the 3min immersion treatment after, heel in the seedbed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910094880A CN101637167A (en) | 2009-08-26 | 2009-08-26 | Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910094880A CN101637167A (en) | 2009-08-26 | 2009-08-26 | Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101637167A true CN101637167A (en) | 2010-02-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910094880A Pending CN101637167A (en) | 2009-08-26 | 2009-08-26 | Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN101637167A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733996A (en) * | 2013-12-21 | 2014-04-23 | 滕运舜 | Cultivation method of sugarcane seedlings |
| CN104542296A (en) * | 2015-01-16 | 2015-04-29 | 广东省农业科学院作物研究所 | Open rooting method for sugarcane tissue culture seedlings |
-
2009
- 2009-08-26 CN CN200910094880A patent/CN101637167A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733996A (en) * | 2013-12-21 | 2014-04-23 | 滕运舜 | Cultivation method of sugarcane seedlings |
| CN104542296A (en) * | 2015-01-16 | 2015-04-29 | 广东省农业科学院作物研究所 | Open rooting method for sugarcane tissue culture seedlings |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100203 |