CN103719150A - Preparation method of selenium-rich biogenic nanometer antibacterial growth regulator - Google Patents
Preparation method of selenium-rich biogenic nanometer antibacterial growth regulator Download PDFInfo
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- CN103719150A CN103719150A CN201310745481.4A CN201310745481A CN103719150A CN 103719150 A CN103719150 A CN 103719150A CN 201310745481 A CN201310745481 A CN 201310745481A CN 103719150 A CN103719150 A CN 103719150A
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Abstract
The invention discloses a preparation method of a selenium-rich biogenic nanometer antibacterial growth regulator. The regulator prepared by the method disclosed by the invention is used for soaking mung bean seeds in comparison to a compound agent consisting of oligochitosan, sodium selenite and agricultural streptomycin under the same concentration condition. Comparison of the test results shows that the germination rate of mung bean sprouts treated with nanometer preparation of the same concentration is increased, the germination potential is increased by 20.8 percent, the water content is increased by 14.1 percent, the selenium content is increased by 83.6 percent, and the acting effect of a low-concentration nanometer preparation is superior to a high-concentration agent. Meanwhile, the particle diameters of nanoparticles of the regulator prepared by the method are less than 500 nanometers and are smaller than that of the agent, the nanoparticles are dispersed uniformly, a phase is pure, and impurities do not exist. Thus, by using the prepared agent prepared by the method, the functions of the agent (oligochitosan, sodium selenite and agricultural streptomycin) are kept, the acting effect is enhanced, the using amount is reduced, so that the prepared agent is very high in application value and very wide in market prospect.
Description
Technical field
The present invention relates to a kind of preparation method of growth regulator, particularly the preparation method of a kind of Se-enriched bio source nano-antibacterial growth regulator.
Background technology
Nano biological preparation, is to use nanotechnology treatments biogenic bioactivator, changes its particle diameter, and then improve material physicochemical property, to increase, it absorbs, transformation efficiency, improves bioavilability, and plays to a certain extent the effect that improves crop quality, increases output.
Chitosan oligosaccharide (chito-oligosaccharide, CS), the straight or branched chitosan hydrolyzate oligomer being connected to form by glycosidic bond by 2 ~ 12 monose, it is the alkaline amino-oligosacchride of unique a large amount of existence in natural sugar, as biogenic performance oligosaccharides, compared more unique function character with chitin and chitosan: water-soluble, humidity-preserving type, antibiotic property, antitumor and immune promotion.The sixties in 20th century, the basis that chitosan oligosaccharide is used as plant immune activity factor begins one's study.Ayers etc., find that in 1976 the oligosaccharides fragment energy inducing plant protective plant protecting agent (phytoalexin) of cell wall is synthetic.Albersheim in 1985, these new ideas of oligosaccharide (oligosaccharins) and frontier have been proposed first, and think that oligosaccharide has regulating plant growth, growth, breeding, diseases prevention and the function such as disease-resistant, immune system response that can stimulating plant, every kind of active oligomeric sugar can send the information that regulates specific function, activate defense reaction and regulating plant growth, produce the active substance with disease resistance, suppress the formation of disease.Active oligomeric sugar is that a class has a fixed structure and bioactive polymer, and studying in the world more is the oligosaccharides segment of plant and the degraded of microorganism wall polysaccharide.Molecular weight is high lower than 10000 chitosan oligosaccharide solvability, has been widely used and plant growth regulating and the agriculture field such as disease-resistant.
Selenium (Se), 1817 Nian Bei Sweden chemist Berzelius discoveries, oxygen group elements, exist with inorganic states selenium and Organic Selenium form at occurring in nature.Research shows, selenium has anti-cancer, antitumor, anti-AIDS and anti-ageing function, it is the essential nutritive element of humans and animals, it is the constituent of glutathione peroxidase (GSH-Px), and the selenium amount of taking the photograph that China is grown up every day is only 26.63 μ g, well below Chinese Soclety of Nutrition and the international selenium recommendation daily intaking amount 50 μ g of association and 60 μ g.Only depend on selenium in natural food to be difficult to meet the normal need of human body, only have inorganic selenium is converted into Organic Selenium, just have general edible and health care and be worth, it is the crux factor that improves food chain selenium level that crop is executed to selenium.Selenium can strengthen plant opposing heavy metal pollution, damage by disease and insect invasion and attack and adverse circumstance and assist urgent ability, and the multi-functional compound selenium fertilizer of development and Application improves to make Se content and the most economical effective approach that improves crop yield in object by being.In plant corpus, a small amount of selenium can be eliminated oxygen radical, and Promoting plant growth, improves fruit quality, and fertilizer adds selenium can make crop yield.
Agricultural streptomycin is the metabolite that actinomycetes produce, and is also a kind of biogenic preparation, and fungicidal spectrum is wide, particularly effective to various bacterial disease, and fungi is also had to preventive and therapeutic effect, has systemic action, can be penetrated in plant corpus, and be transmitted to other positions.To people and animals' low toxicity, also very little to fish and aquatile toxicity.Agricultural streptomycin is generally used for crop land for growing field crops, the control of greengrocery crop bacteriosis.
And also do not have now a kind of can be by above-mentioned three kinds of raw material organic combinations, make it on the basis of performance self function, can reach synergy, strengthen the growth regulator of efficiency effect.
Summary of the invention
The present invention is in order to solve the existing above-mentioned deficiency of prior art, propose a kind ofly can obtain promotion plant growth, the method with the Se-enriched bio source nano-antibacterial growth regulator of sterilization antibiotic effect, the method can also allow its each component act synergistically simultaneously, strengthens efficiency.
Technical solution of the present invention is: the preparation method of a kind of Se-enriched bio source nano-antibacterial growth regulator, is characterized in that: described method is carried out according to following steps:
A, getting solubility chitosan oligosaccharide, to be dissolved in concentration be in 3% acetic acid, and be stirred to abundant dissolving, the chitosan oligosaccharide acetic acid solution that to obtain concentration be 2ppm,
B, get sodium selenite and be dissolved in distilled water, the sodium selenite solution that to obtain concentration be 2.5ppm,
C, according to the volume ratio of 5:4, add the beaker in ice bath to form mixed solution described chitosan oligosaccharide acetic acid solution and sodium selenite solution simultaneously, then the pH value of mixed solution be adjusted to 4.5-5.7,
D, to the soybean lecithin that adds 1.5mg/ml in the mixed solution after c step process, the soybean lecithin volume adding and the ratio of mixed liquor volume are 1:4, ice bath magnetic agitation 25-35min under the condition of 3000r/min, then the agricultural streptomycin solution that adds wherein 10ppm, continue magnetic agitation 15min, the volume of the agricultural streptomycin solution adding and the volume ratio of chitosan oligosaccharide acetic acid solution are 2.5-3.5:5
E, the solution after d step process is added to the beaker that is arranged in ultrasonic cleaning machine, adition process continues 9-10min, this solution impulse ultrasound under the condition of power 85%, On 5s/Off 3s is processed to 2h, then standing 15min, magnetic agitation 30min again, to adding concentration in solution, be 1% STPP solution, add the volume of STPP solution be in c step chitosan oligosaccharide acetic acid solution 1%, continue impulse ultrasound 2h, obtain opalescence look finely dispersed chitosan oligosaccharide-selenium-agricultural streptomycin nanometer formulation, be Se-enriched bio source nano-antibacterial growth regulator.
Compared with the existing technology, tool has the following advantages in the present invention:
Utilize the prepared conditioning agent of the method disclosed in the present, with chitosan oligosaccharide, sodium selenite, agricultural streptomycin compound former dose with under concentration conditions, mung bean being made to seed soaking, comparative test result is that the mung bean sprouts germination rate that same concentration nanometer formulation is processed improves, germination vigor increases by 20.8 %, water content increases by 14.1%, selenium content increases by 83.6%, and the nanometer of low concentration action effect processed is better than former dose of high concentration.Utilize the particle diameter of the nano particle of conditioning agent prepared by this method to be less than 500nm simultaneously, less than former dose of particle diameter, be uniformly dispersed, and thing is mutually pure, free from admixture exists.Therefore utilize the prepared preparation of this method not only to retain the function of former dose (chitosan oligosaccharide, sodium selenite, agricultural streptomycin), more strengthened its action effect, and reduce consumption, have very large using value, its market prospects are very wide.
Accompanying drawing explanation
Fig. 1 is the light microscopic figure (* 40) of former dose used of the inventive method.
Fig. 2 is the SEM figure of former dose used of the inventive method.
Fig. 3 is the nano particle light microscopic figure (* 40) of the conditioning agent of the inventive method acquisition.
Fig. 4 is the nano particle SEM figure of the conditioning agent of the inventive method acquisition.
Fig. 5 is conditioning agent and the former dose of mung bean sprouts growing state comparison diagram that the inventive method obtains.
Embodiment
Below in conjunction with the embodiment of the accompanying drawing explanation embodiment of the present invention, as shown in Figures 1 to 5: the preparation method of a kind of Se-enriched bio source nano-antibacterial growth regulator, carries out according to following steps:
First get solubility chitosan oligosaccharide and be dissolved in acetic acid, and be stirred to chitosan oligosaccharide and be fully dissolved in acetic acid, selecting concentration is here 3% acetic acid, and finally obtaining concentration is the chitosan oligosaccharide acetic acid solution of 2ppm,
Then get sodium selenite and be dissolved in distilled water, the sodium selenite solution that acquisition concentration is 2.5ppm,
According to the volume ratio of 5:4, add the beaker in ice bath to form mixed solution the chitosan oligosaccharide acetic acid solution obtaining and sodium selenite solution simultaneously above, then the pH value of mixed solution be adjusted to 4.5-5.7,
In the mixed solution after above-mentioned processing, add the soybean lecithin of 1.5mg/ml as dispersant, and the soybean lecithin volume adding and the ratio of mixed liquor volume are 1:4, mixed solution ice bath magnetic agitation 25-35min under the condition of 3000r/min of dispersant will have been added, then the agricultural streptomycin solution that adds wherein 10ppm, continue magnetic agitation 15min, the volume of the agricultural streptomycin solution adding is 2.5 to 3.5 to 5 with the volume ratio of chitosan oligosaccharide acetic acid solution
Solution after above-mentioned steps is processed slow (being preferably dropwise) is added to the beaker that is arranged in ultrasonic cleaning machine, adition process continues 9-10min, to the solution in beaker at power 85%, under the condition of On 5s/Off 3s, impulse ultrasound is processed 2h, then standing 15min, magnetic agitation 30min again afterwards, to adding concentration in solution, be 1% STPP, and the volume of the STPP adding is 1% of chitosan oligosaccharide acetic acid solution volume, continue impulse ultrasound 2h, obtain opalescence look finely dispersed chitosan oligosaccharide-selenium-agricultural streptomycin nanometer formulation, be Se-enriched bio source nano-antibacterial growth regulator.
Embodiment 1:
With analytical balance, weigh 0.2mg solubility chitosan oligosaccharide, be dissolved into 3% acetic acid solution, the chitosan oligosaccharide acetic acid solution that acquisition concentration is 2ppm.
With analytical balance, weigh 0.25mg sodium selenite, by its solution in distilled water, the sodium selenite solution that to obtain concentration be 2.5ppm.
By two kinds of solution of above-mentioned concentration, according to the volume ratio of 5:4, (then chitosan oligosaccharide acetic acid solution: sodium selenite solution=5:4) add in beaker simultaneously and obtain mixed solution, beaker is placed in ice bath regulates mixed solution pH to 4.5～5.7 with 0.1% NaOH.
In mixed solution, add 1.5mg/ml soybean lecithin to make dispersant, / 4th of a volume that the volume that adds soybean lecithin is mixed solution, then ice bath magnetic agitation 25-35min under the condition of 3000r/min, again to the agricultural streptomycin solution that adds 10ppm in mixed solution, the volume of the agricultural streptomycin solution that added is here half of chitosan oligosaccharide acetic acid solution volume, continues to stir 15min.
The solution obtaining after doing drilling dropwise adds the beaker that is placed in ultrasonic cleaning machine, going through 10min adds completely, and at power 85%, impulse ultrasound 2h under the condition of On 5s/Off 3s, then standing 15min, and magnetic agitation 30min, to adding concentration in beaker, be 1% STPP solution again, the amount adding is 1% of chitosan oligosaccharide acetic acid solution, continue impulse ultrasound 2h, obtain opalescence look finely dispersed chitosan oligosaccharide-selenium-agricultural streptomycin nanometer formulation, be Se-enriched bio source nano-antibacterial growth regulator.
The impact that utilizes the prepared Se-enriched bio source nano-antibacterial growth regulator of the method for the invention to cultivate mung bean sprouts.
The mung bean seed of selecting full seed is some, clear water is cleaned, with the emerge in worm water of 55 ℃ of left and right 5 minutes, the seed of washing and sterilizing is soaked in respectively and is utilized in former dose of conditioning agent prepared by the method for the invention and the same concentration being comprised of chitosan oligosaccharide, sodium selenite, agricultural streptomycin, under room temperature, soak seed 6～8 hours.
Respectively the mung bean seed after two kinds of reagent seed soakings is respectively selected to 90, the culture dish of two layers of filter paper that has been placed in paving after sterilization, shading moisturizing is cultivated, and every 4～6 hours water sprays once, are cultivated 5～7 days.
Result of study data see the following form:
Germination vigor is the ratio of concentrating maximum one day normal seed sprouting number of germinative number and seed sum after seed germination; Germination rate is the normal seed grain number germinateing and the ratio that supplies to plant experimentally seed number in regulation germination rate mensuration number of days.
Mung bean sprouts germination rate under table one Se-enriched bio source nano-antibacterial growth regulator and former dose of cultivation
Table one explanation, the mung bean germination vigor after nanometer conditioning agent is processed is better than former dose for the treatment of effect, than former dose of germination vigor of same concentration, increases by 20.8%.
Table two Se-enriched bio source nano-antibacterial growth regulator and former dose long to mung bean sprouts bud, the cultivation situation (unit: cm) that bud is thick
Table two explanation, this conditioning agent not only can promote the growth of mung bean sprouts, can also effectively improve absorption efficiency and availability, saves medication.
Table three Se-enriched bio source nano-antibacterial growth regulator and the former dose of situation that affects of mung bean sprouts being cultivated to fresh weight, dry weight
| ? | Former dose | Nanometer agent |
| Fresh weight/g | 12.0 | 13.5 |
| Dry weight/g | 1.72 | 1.77 |
| Water content/% | 85.7% | 86.9% |
This conditioning agent of table three explanation has not only retained former dose of effect that increases mung bean sprouts water content, has also strengthened on this basis this action effect.
With bean sprouts's basal part of stem separately, drying and crushing, crosses 80 wood sieves, gets 0.1g, utilizes its selenium content of ultraviolet colorimetric method for determining.Its selenium content can reach 1.682mg/kg.
The method disclosed in the present, can obtain Se-enriched bio source nano-antibacterial growth regulator, and said preparation can obviously promote the growth of mung bean sprouts, improves selenium content, effectively improves mung bean sprouts quality, and fewer than former dose of consumption, action effect is obvious, economy, safety.
Claims (1)
1. a preparation method for Se-enriched bio source nano-antibacterial growth regulator, is characterized in that: described method is carried out according to following steps:
A, getting solubility chitosan oligosaccharide, to be dissolved in concentration be in 3% acetic acid, and be stirred to abundant dissolving, the chitosan oligosaccharide acetic acid solution that to obtain concentration be 2ppm,
B, get sodium selenite and be dissolved in distilled water, the sodium selenite solution that to obtain concentration be 2.5ppm,
C, according to the volume ratio of 5:4, add the beaker in ice bath to form mixed solution described chitosan oligosaccharide acetic acid solution and sodium selenite solution simultaneously, then the pH value of mixed solution be adjusted to 4.5-5.7,
D, to the soybean lecithin that adds 1.5mg/ml in the mixed solution after c step process, the soybean lecithin volume adding and the ratio of mixed liquor volume are 1:4, ice bath magnetic agitation 25-35min under the condition of 3000r/min, then the agricultural streptomycin solution that adds wherein 10ppm, continue magnetic agitation 15min, the volume of the agricultural streptomycin solution adding and the volume ratio of chitosan oligosaccharide acetic acid solution are 2.5-3.5:5
E, the solution after d step process is added to the beaker that is arranged in ultrasonic cleaning machine, adition process continues 9-10min, this solution impulse ultrasound under the condition of power 85%, On 5s/Off 3s is processed to 2h, then standing 15min, magnetic agitation 30min again, to adding concentration in solution, be 1% STPP solution, add the volume of STPP solution be in c step chitosan oligosaccharide acetic acid solution 1%, continue impulse ultrasound 2h, obtain opalescence look finely dispersed chitosan oligosaccharide-selenium-agricultural streptomycin nanometer formulation, be Se-enriched bio source nano-antibacterial growth regulator.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357628A (en) * | 2020-03-24 | 2020-07-03 | 袁友梅 | Organic mung bean seedlings and efficient cultivation process thereof |
| CN112047777A (en) * | 2020-07-24 | 2020-12-08 | 范雨生 | Preparation method and application of plant cell gene potential awakening agent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102037962A (en) * | 2010-11-29 | 2011-05-04 | 辽宁师范大学 | Chitosan nano-biological preparation, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102037962A (en) * | 2010-11-29 | 2011-05-04 | 辽宁师范大学 | Chitosan nano-biological preparation, and preparation method and application thereof |
Non-Patent Citations (3)
| Title |
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| 刘美静等: "纳米壳聚糖对金属离子的吸附研究", 《安徽农学通报》 * |
| 童春义等: "壳寡糖-硒纳米植物营养调节剂的研制与应用", 《湖南农业大学学报（自然科学版）》 * |
| 赵玲玲等: "纳米壳聚糖对小麦种苗生长的影响", 《安徽农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357628A (en) * | 2020-03-24 | 2020-07-03 | 袁友梅 | Organic mung bean seedlings and efficient cultivation process thereof |
| CN112047777A (en) * | 2020-07-24 | 2020-12-08 | 范雨生 | Preparation method and application of plant cell gene potential awakening agent |
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