CN103718959A - 寒兰组培苗的快速繁殖方法 - Google Patents
寒兰组培苗的快速繁殖方法 Download PDFInfo
- Publication number
- CN103718959A CN103718959A CN201310696836.5A CN201310696836A CN103718959A CN 103718959 A CN103718959 A CN 103718959A CN 201310696836 A CN201310696836 A CN 201310696836A CN 103718959 A CN103718959 A CN 103718959A
- Authority
- CN
- China
- Prior art keywords
- sponge
- culture
- liquid nutrient
- nutrient medium
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 13
- 241000173256 Cymbidium kanran Species 0.000 title abstract 6
- 239000000463 material Substances 0.000 claims abstract description 30
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 229920001817 Agar Polymers 0.000 claims abstract description 9
- 239000008272 agar Substances 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 7
- 239000002609 medium Substances 0.000 claims description 40
- 235000015097 nutrients Nutrition 0.000 claims description 35
- 239000007788 liquid Substances 0.000 claims description 31
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000009395 breeding Methods 0.000 claims description 11
- 230000001488 breeding effect Effects 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000005648 plant growth regulator Substances 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000009630 liquid culture Methods 0.000 abstract 3
- 230000003670 easy-to-clean Effects 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 150000004053 quinones Chemical class 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Hydroponics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310696836.5A CN103718959B (zh) | 2013-12-18 | 2013-12-18 | 寒兰组培苗的快速繁殖方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310696836.5A CN103718959B (zh) | 2013-12-18 | 2013-12-18 | 寒兰组培苗的快速繁殖方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103718959A true CN103718959A (zh) | 2014-04-16 |
CN103718959B CN103718959B (zh) | 2016-02-10 |
Family
ID=50443456
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310696836.5A Expired - Fee Related CN103718959B (zh) | 2013-12-18 | 2013-12-18 | 寒兰组培苗的快速繁殖方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103718959B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103734008A (zh) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | 一种蝴蝶兰组织培育快速繁殖用液体培养基及相应培养方法 |
CN106879474A (zh) * | 2017-04-21 | 2017-06-23 | 山东省农作物种质资源中心 | 一种寒兰种质资源离体保存及保存后恢复生长的方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100751951B1 (ko) * | 2006-08-09 | 2007-08-27 | 강경원 | 양란 심비디움 신품종 잔묵(殘默) |
CN102379242A (zh) * | 2011-08-29 | 2012-03-21 | 张笑逸 | 中国地生兰花原生细胞胚超声波破壁培养法 |
CN103329706A (zh) * | 2013-06-25 | 2013-10-02 | 徐玉文 | 一种吊兰匍枝扦插生长器 |
-
2013
- 2013-12-18 CN CN201310696836.5A patent/CN103718959B/zh not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100751951B1 (ko) * | 2006-08-09 | 2007-08-27 | 강경원 | 양란 심비디움 신품종 잔묵(殘默) |
CN102379242A (zh) * | 2011-08-29 | 2012-03-21 | 张笑逸 | 中国地生兰花原生细胞胚超声波破壁培养法 |
CN103329706A (zh) * | 2013-06-25 | 2013-10-02 | 徐玉文 | 一种吊兰匍枝扦插生长器 |
Non-Patent Citations (4)
Title |
---|
A.R.ROY ET AL.: "TDZ induced micropropagation in Cymbidium giganteum Wall.Ex Lindl. and assessment of genetic variation in the regenerated plants", 《PLANT GROWTH REGUL》, vol. 68, 31 December 2012 (2012-12-31), pages 435 - 445 * |
徐程等: "中国兰的组织培养", 《植物生理学通讯》, vol. 38, no. 2, 30 April 2002 (2002-04-30) * |
朱国兵等: "寒兰的快速繁殖技术", 《热带亚热带植物学报》, vol. 14, no. 2, 31 December 2006 (2006-12-31) * |
陈其皎等: "载体式培养基的制备与应用", 《食用菌》, no. 5, 31 December 2002 (2002-12-31) * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103734008A (zh) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | 一种蝴蝶兰组织培育快速繁殖用液体培养基及相应培养方法 |
CN103734008B (zh) * | 2013-12-26 | 2016-04-13 | 广西森仙源生物科技有限公司 | 一种蝴蝶兰组织培育快速繁殖用液体培养基及相应培养方法 |
CN106879474A (zh) * | 2017-04-21 | 2017-06-23 | 山东省农作物种质资源中心 | 一种寒兰种质资源离体保存及保存后恢复生长的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN103718959B (zh) | 2016-02-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103718961B (zh) | 牛樟芝扦插枝存活率高的海绵液体培养基及其应用 | |
CN102577976B (zh) | 一种构树简便组培方法 | |
CN105684733B (zh) | 袋栽金针菇培育方法 | |
CN103125396B (zh) | 一种山药苗离体繁殖方法 | |
CN107018896B (zh) | 一种设施化扦插南京椴的方法 | |
CN101790962B (zh) | 一种铁线蕨的生产方法 | |
CN103733992B (zh) | 树葡萄组培苗快速繁殖的海绵液体培养基及其应用 | |
CN104186202B (zh) | 一种工厂化生产香菇透气菌包的方法 | |
CN103798144A (zh) | 红椿组织培养培养基 | |
CN104969756B (zh) | 紫薇嫩枝容器扦插方法 | |
CN103718960A (zh) | 树葡萄快速结果的培育方法 | |
CN103694012B (zh) | 一种用于好氧发酵的可调节供氧系统 | |
CN103098712A (zh) | 一种高山羊齿的繁育方法 | |
CN102960243A (zh) | 一种利用中国石蒜不带基盘鳞片组培快繁的方法 | |
CN103733991B (zh) | 牛樟芝扦插枝的培育方法 | |
CN104303840A (zh) | 一种盘装金针菇的栽培方法 | |
CN103704012B (zh) | 滑子蘑菌块和菌块生产工艺及其专用设备 | |
CN103718959A (zh) | 寒兰组培苗的快速繁殖方法 | |
CN106489700A (zh) | 一种扦插育苗苗床 | |
CN103718958B (zh) | 寒兰组培苗快速繁殖的海绵液体培养基及其应用 | |
CN107494269A (zh) | 一种消除小花万代兰组织培养过程中内生菌的方法 | |
CN105052559A (zh) | 一种利用蚯蚓粪栽培大杯蕈的方法 | |
CN105130686B (zh) | 一种培养料及其制备方法和培养料在黄背木耳栽培中的应用 | |
CN104067937B (zh) | 一种潮汐式生物反应器及其培养百合籽球的方法 | |
CN104620989B (zh) | 培育苦瓜再生苗的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170918 Address after: Calculation of business R & D center eleven layer research room 530029 Nanning city the Guangxi Zhuang Autonomous Region Qingxiu District Bauhinia Road No. 1 Nanning international internet dedicated channel and cloud Patentee after: GUANGXI YIMU AGRICULTURAL TECHNOLOGY Co.,Ltd. Address before: 545002, the Guangxi Zhuang Autonomous Region, Liuzhou North Long Pond flower demonstration Office Patentee before: LIUZHOU TIANZI HORTICULTURE Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160210 |
|
CF01 | Termination of patent right due to non-payment of annual fee |