CN103691157B - The chromatographic column that flow velocity is controlled - Google Patents
The chromatographic column that flow velocity is controlled Download PDFInfo
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- CN103691157B CN103691157B CN201310644877.XA CN201310644877A CN103691157B CN 103691157 B CN103691157 B CN 103691157B CN 201310644877 A CN201310644877 A CN 201310644877A CN 103691157 B CN103691157 B CN 103691157B
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Abstract
The invention provides the controlled chromatographic column of a kind of flow velocity, belong to biologically pure instrument field. Chromatographic column provided by the invention, cylinder top arranges U-shaped screen cloth, is convenient to the liquid such as buffer solution, sample liquid, eluent and flows down from the wall of cylinder, avoids causing and splashes; Bottom at liquid outlet arranges flow speed controller, can control flow velocity by flow speed controller, and can directly read flow velocity, facilitate experimental implementation, flow velocity in chromatography operation is quantized, and the purification process being conducive to carry out identical or similar protein later provides reference role; Column cap and cylinder, cylinder and post tail all can split, and it are assembled before chromatography, after chromatography, can mutually take apart, clean respectively, the processing such as sterilizing. The present invention is simple in structure, easy to operate, and the purification effect of albumen is good.
Description
Technical field
The invention belongs to biologically pure apparatus, be specifically related to the controlled chromatographic column of a kind of flow velocity.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, is mainly to adopt column chromatography, uses chromatographic column, realizes by column chromatography process. traditional column chromatography is the top that mixing cpd is placed on to glass column post bed, make the eluant, eluent (mobile phase) of mixed solvent composition under Action of Gravity Field, by the vertical glass column of absorbent media material (fixing phase) is housed, separation obtains each purer product (sterling), its principle is: because each component in mixing cpd is to fixing different with the affinity of mobile phase mutually in chromatographic column, therefore select suitable fixing phase and mobile phase according to the character of mixing cpd, can reach the object that wherein each component separates as far as possible, the product separating is collected into respectively in each receiving flask in pillar outlet, then, identify the chromatographic solution of collecting in each receiving flask, merge and concentrated each identical chromatographic solution according to qualification result, obtain each sterling.
There is following problem in current chromatographic column: 1. the flow velocity that can not control liquid in the time carrying out chromatography operation, if the flow velocity of sample liquid is too fast, be easy to cause in destination protein and cylinder filler time of contact too short and should not be adsorbed, if the flow velocity of eluent is too fast, be also not easy destination protein exchange to wash; If flow velocity is excessively slow, can increase again the chromatography time, due to not being quantized of flow velocity, in the time processing same or similar albumen next time, cannot provide reference; 2. in the time of sample introduction, sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column. The filler of using due to chromatography is usually more expensive, and the filler splashing on inwall is not fully utilized in chromatography, affects chromatography effect.
Summary of the invention
For addressing the above problem, the invention provides a kind of detachable chromatographic column, adopt following technical scheme:
The chromatographic column that flow velocity is controlled, comprises column cap and cylinder, and described column cap, is characterized in that for being threaded with cylinder, and the top of described column cap is provided with the through hole passing through for silica gel hose; The top of described cylinder arranges dismountable U-shaped screen cloth, and described U-shaped screen cloth is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth; The bottom of described cylinder is threaded connection with post tail and is connected, and the bottom of described post tail is provided with liquid outlet; The top of described liquid outlet arranges sieve plate, and the aperture of described sieve plate is 0.2 ~ 0.45 μ m; The bottom of described liquid outlet arranges flow speed controller.
Preferably, the junction of the junction of described column cap and cylinder, cylinder and post tail is provided with sealing ring, and described sealing ring is silica gel material.
Preferably, the top of described sieve plate arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder.
The present invention has following beneficial effect:
1. chromatographic column provided by the invention, dismountable U-shaped screen cloth is set at the top of cylinder, the horn shape that described U-shaped screen cloth is upper end open, lower end closed, and the sidewall of U-shaped screen cloth contains through hole, so setting, when being conducive to buffer solution, sample liquid, eluent and entering from cylinder top, be to flow down along the wall of cylinder, can not cause splashing of the interior liquid of cylinder, cause that filler splashes on the wall of cylinder, affect packed height, thereby affect the purification effect of albumen.
2. chromatographic column provided by the invention, in the bottom of cylinder, post tail is set, and on the inside of post tail, the top of liquid outlet, sieve plate is set, and the aperture that limits sieve plate is 0.2 ~ 0.45 μ m, and cellulose acetate film is set on sieve plate as optimal way, filler so so just can reach protein purification time not can along with buffer solution, sample liquid, eluent together, run off from conical outlet, but can not affect buffer solution, sample liquid and the eluent outflow effect from conical outlet; In addition, sieve plate is arranged on post tail, after purifying completes, can opens post tail, take out easily the filler in cylinder.
3. chromatographic column provided by the invention, middling speed qualitative filter paper filter membrane is placed on the top of U-shaped screen cloth, can carry out initial gross separation to sample liquid to be separated, and the impurity such as the untreated thalline completely that interception size ratio is larger, can also avoid chromatography sample to stop up U-shaped screen cloth.
4. chromatographic column provided by the invention, in the junction of junction, cylinder and the post tail of column cap and cylinder, be all provided with sealing ring, be convenient to the airtight of column cap and cylinder, cylinder and post tail, avoid buffer solution, sample liquid, eluent to flow out from both gaps, cause waste.
5. chromatographic column provided by the invention, bottom at liquid outlet arranges flow speed controller, can control flow velocity by flow speed controller, and can directly read flow velocity, facilitate experimental implementation, flow velocity in chromatography operation is quantized, and the purification process being conducive to carry out identical or similar protein later provides reference role.
6. chromatographic column provided by the invention, column cap and cylinder, cylinder and post tail all can split, and it are assembled before chromatography, after chromatography, can mutually take apart, clean respectively, the processing such as sterilizing. The present invention is simple in structure, easy to operate, and the purification effect of albumen is good.
Brief description of the drawings
Fig. 1 is structural representation of the present invention; Wherein, 1 represents column cap; 2 represent silica gel hose; 3 represent liquid outlet; 4 represent flow speed controller; 5 represent sieve plate; 6 represent U-shaped screen cloth; 7 represent cylinder; 8 represent post tail.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described. Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of requirement of the present invention.
As shown in Figure 1, the chromatographic column that a kind of flow velocity is controlled, comprises column cap 1 and cylinder 7, and described column cap 1, is characterized in that for being threaded with cylinder 7, and the top of described column cap 1 is provided with the through hole passing through for silica gel hose 2; The top of described cylinder 7 arranges dismountable U-shaped screen cloth 6, and described U-shaped screen cloth 6 is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth 6 contains through hole; Middling speed qualitative filter paper filter membrane (do not mark in the drawings, it is arranged on the top of screen cloth, but is no more than the horizontal level at cylinder top) is placed on the top of described U-shaped screen cloth 6, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth 6; The bottom of described cylinder 7 is threaded connection with post tail 8 and is connected, and the bottom of described post tail 8 is provided with liquid outlet 3; The top of described liquid outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.2 ~ 0.45 μ m; The bottom of described liquid outlet 3 arranges flow speed controller 4.
Preferably, the junction of the junction of described column cap 1 and cylinder 7, cylinder 7 and post tail 8 is provided with sealing ring, and (not marking out in the drawings), for sealing function, described sealing ring is silica gel material, sealing effectiveness is better.
Preferably, the top of described sieve plate 5 arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder 7.
Claims (1)
1. the controlled chromatographic column of flow velocity, comprises column cap (1) and cylinder (7), and described column cap (1), is characterized in that for being threaded with cylinder (7), and the top of described column cap (1) is provided with the through hole passing through for silica gel hose (2); The top of described cylinder (7) arranges dismountable U-shaped screen cloth (6), and described U-shaped screen cloth (6) is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth (6) contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth (6), and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth (6); The bottom of described cylinder (7) is threaded connection and post tail (8)
Connect, the bottom of described post tail (8) is provided with liquid outlet (3); The top of described liquid outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.2 ~ 0.45 μ m; The bottom of described liquid outlet (3) arranges flow speed controller (4);
The junction of junction, cylinder (7) and the post tail (8) of described column cap (1) and cylinder (7) is provided with sealing ring, and described sealing ring is silica gel material;
The top of described sieve plate (5) arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644877.XA CN103691157B (en) | 2013-12-05 | 2013-12-05 | The chromatographic column that flow velocity is controlled |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644877.XA CN103691157B (en) | 2013-12-05 | 2013-12-05 | The chromatographic column that flow velocity is controlled |
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| Publication Number | Publication Date |
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| CN103691157A CN103691157A (en) | 2014-04-02 |
| CN103691157B true CN103691157B (en) | 2016-05-11 |
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| CN201310644877.XA Active CN103691157B (en) | 2013-12-05 | 2013-12-05 | The chromatographic column that flow velocity is controlled |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN201410310Y (en) * | 2009-03-17 | 2010-02-24 | 温超 | Chromatographic column with scales |
| CN202410236U (en) * | 2011-12-28 | 2012-09-05 | 上海师范大学 | Detachable chromatographic column with scales |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| CN202762139U (en) * | 2012-04-24 | 2013-03-06 | 齐齐哈尔大学 | Chromatographic column flowing phase device |
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN203315795U (en) * | 2013-06-03 | 2013-12-04 | 荣捷生物工程（苏州）有限公司 | Chromatographic column device |
| CN203678015U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Flow velocity controllable chromatographic column |
-
2013
- 2013-12-05 CN CN201310644877.XA patent/CN103691157B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN201410310Y (en) * | 2009-03-17 | 2010-02-24 | 温超 | Chromatographic column with scales |
| CN202410236U (en) * | 2011-12-28 | 2012-09-05 | 上海师范大学 | Detachable chromatographic column with scales |
| CN202762139U (en) * | 2012-04-24 | 2013-03-06 | 齐齐哈尔大学 | Chromatographic column flowing phase device |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| CN203315795U (en) * | 2013-06-03 | 2013-12-04 | 荣捷生物工程（苏州）有限公司 | Chromatographic column device |
| CN203678015U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Flow velocity controllable chromatographic column |
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| CN103691157A (en) | 2014-04-02 |
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