CN103691150B - A kind of detachable chromatographic column - Google Patents
A kind of detachable chromatographic column Download PDFInfo
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- CN103691150B CN103691150B CN201310643984.0A CN201310643984A CN103691150B CN 103691150 B CN103691150 B CN 103691150B CN 201310643984 A CN201310643984 A CN 201310643984A CN 103691150 B CN103691150 B CN 103691150B
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Abstract
The invention provides a kind of detachable chromatographic column, belong to biologically pure instrument field.Chromatographic column provided by the invention, cylinder arranges scale, and handled easily personnel are to the grasp of packed height and sample size; Cylinder top arranges U-shaped screen cloth, is convenient to the liquid such as buffer solution, sample liquid, eluent and flows down from the wall of cylinder, avoid causing splashing.Chromatographic column provided by the invention, column cap and cylinder, cylinder and post tail all can split, and assembles before chromatography, after chromatography, can mutually take apart it, carry out respectively cleaning, the process such as sterilizing.Structure of the present invention is simple, and easy to operate, the purification effect of albumen is good.
Description
Technical field
The invention belongs to biologically pure apparatus, be specifically related to a kind of detachable chromatographic column.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, mainly adopts column chromatography, uses chromatographic column, is realized by column chromatography procedure.Traditional column chromatography is top mixing cpd being placed on glass column post bed, the eluant, eluent (mobile phase) that mixed solvent is formed under gravity, by being equipped with the vertical glass post of absorbent media material (Stationary liquid), be separated and obtain each purer product (sterling), its principle is: because component each in mixing cpd is different with the affinity of mobile phase to Stationary liquid in chromatographic column, therefore select suitable Stationary liquid and mobile phase according to the character of mixing cpd, the object wherein each component be separated as far as possible can be reached, the product be separated is collected in each receiving flask at column outlet respectively, then, identify the chromatographic solution collected in each receiving flask, merge and concentrated each identical chromatographic solution according to qualification result, obtain each sterling.
Common chromatographic column can be divided into normal pressure post, middle compression leg and high-pressure column according to pressure difference.Normal pressure post is with the most use in practice, is usually oneself to adopt the chromatographic column of different materials as required, load the separation and purification that different fillers carrys out realize target material.Glass chromatography column is especially conventional again, often comprises cylinder, piston, and what have also comprises the barb etc. for fixing liquid feeding ball being positioned at cylinder upper end.Glass chromatography column common at present exists following not enough: on the one hand, in the filler filling and the filling of separated object quality sample of glass chromatography column, usually adopt different fillers according to different filler and different material, sample ratio, be beneficial to save filler while reaching optimal separation effect, and filler. the ratio of sample is usually with reference to its volume ratio, but conventional glass column does not have scale at present, usually need operating personnel to estimate by rule of thumb or measure with other measurement facilities, bothering very much; On the other hand, in chromatography process, usually need to be grasped the migration velocity of sample in separating medium and filler, then the speed adjustment of efflux is carried out according to migration velocity, be beneficial to save the chromatography time while reaching optimal separation effect, but current chromatographic column does not have scale, usually mark at the position marking pen of different time points to certain chromatography colour band, such way is not accurate enough, the mark that marker pen marks simultaneously may be washed because of leakage during interpolation eluant, eluent, need again to mark, very inconvenient.
In addition, current chromatographic column is when sample introduction, and sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column.Filler due to chromatography is usually costly, and the filler splashed on inwall is not fully utilized in chromatography, affects chromatographic effect.
Summary of the invention
For solving the problem, the invention provides a kind of detachable chromatographic column, adopting following technical scheme:
A detachable chromatographic column, comprises column cap and cylinder, and described column cap and cylinder, for being threaded, is characterized in that, the top of described column cap is provided with the through hole passed through for silica gel hose; Described cylinder adopts transparent plastic material, along cylinder axis to being provided with uniform scale on described cylinder; The top of described cylinder arranges dismountable U-shaped screen cloth, and described U-shaped screen cloth is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth; The bottom of described cylinder is threaded connection and is connected with post tail, and the bottom of described post tail is provided with liquid outlet, and the top of described liquid outlet arranges sieve plate, and the aperture of described sieve plate is 0.2 ~ 0.4 μm.
Preferably, the junction of the junction of described column cap and cylinder, cylinder and post tail is provided with sealing ring, and described sealing ring is silica gel material.
Preferably, the top of described sieve plate arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder.
The present invention has following beneficial effect:
1. chromatographic column provided by the invention, its cylinder adopts transparent plastic material, and uniform scale is set on cylinder, setting like this, be conducive to easily and accurately holding packing samples ratio when the filler filling of chromatographic column and sample loading, by observing the graduation position of certain colour band at different time points place, thus the migration rate of the separated material of convenient grasp in chromatographic column, make whole chromatography process more convenient, improve separative efficiency and whole operating efficiency.
2. chromatographic column provided by the invention, at the top of cylinder, dismountable U-shaped screen cloth is set, described U-shaped screen cloth is the horn shape for upper end open, lower end closed, and the sidewall of U-shaped screen cloth contains through hole, so arranges, be conducive to buffer solution, sample liquid, eluent be when entering from cylinder top, be flow down along the wall of cylinder, the splashing of liquid in cylinder can not be caused, cause filler to splash on the wall of cylinder, affect packed height, thus affect the purification effect of albumen.
3. chromatographic column provided by the invention, in the bottom of cylinder, post tail is set, and in the inside of post tail, the top of liquid outlet arranges sieve plate, and the aperture limiting sieve plate is 0.2 ~ 0.4 μm, and cellulose acetate film is set on sieve plate as preferred embodiment, filler when so so just can reach protein purification can not along with buffer solution, sample liquid, eluent together, run off from conical outlet, but buffer solution, sample liquid and the eluent outflow effect from conical outlet can not be affected; In addition, sieve plate is arranged on post tail, after purifying completes, post tail can be opened, take out the filler in cylinder easily.
4. chromatographic column provided by the invention, middling speed qualitative filter paper filter membrane is placed on the top of U-shaped screen cloth, can carry out initial gross separation to sample liquid to be separated, and the impurity such as the untreated thalline completely that interception particle diameter is larger, can also avoid chromatography samples to block U-shaped screen cloth.
5. chromatographic column provided by the invention, in the junction of column cap and cylinder, the junction of cylinder and post tail, be all provided with sealing ring, be convenient to the airtight of column cap and cylinder, cylinder and post tail, avoid buffer solution, sample liquid, eluent to flow out from both gaps, cause waste.
6. chromatographic column provided by the invention, column cap and cylinder, cylinder and post tail all can split, and assembles before chromatography, after chromatography, can mutually take apart it, carry out respectively cleaning, the process such as sterilizing.Structure of the present invention is simple, and easy to operate, the purification effect of albumen is good.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention; Wherein, 1 represents column cap; 2 represent silica gel hose; 3 represent liquid outlet; 4 represent scale; 5 represent sieve plate; 6 represent U-shaped screen cloth; 7 represent cylinder; 8 represent post tail.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described.Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of application claims.
As shown in Figure 1,
A detachable chromatographic column, a kind of detachable chromatographic column, comprises column cap 1 and cylinder 7, and described column cap 1 and cylinder 7, for being threaded, is characterized in that, the top of described column cap 1 is provided with the through hole passed through for silica gel hose 2; Described cylinder 7 adopts transparent plastic material, along cylinder axis to being provided with uniform scale 4 on described cylinder 7; The top of described cylinder 7 arranges dismountable U-shaped screen cloth 6, and described U-shaped screen cloth 6 is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth 6 contains through hole; Middling speed qualitative filter paper filter membrane (do not mark in the drawings, it is arranged on the top of screen cloth, but is no more than the horizontal level at cylinder top) is placed on the top of described U-shaped screen cloth 6, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth 6; The bottom of described cylinder 7 is threaded connection and is connected with post tail 8, and the bottom of described post tail 8 is provided with liquid outlet 3, and the top of described liquid outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.2 ~ 0.4 μm.
Preferably, the junction of the junction of described column cap 1 and cylinder 7, cylinder 7 and post tail 8 is provided with sealing ring, (in the drawings not mark out), and for sealing function, described sealing ring is silica gel material, and sealing effectiveness is better.
Preferably, the top of described sieve plate 5 arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder 7.
Claims (1)
1. a detachable chromatographic column, comprises column cap (1) and cylinder (7), and described column cap (1) and cylinder (7), for being threaded, is characterized in that, the top of described column cap (1) is provided with the through hole passed through for silica gel hose (2); Described cylinder (7) adopts transparent plastic material, along cylinder axis to being provided with uniform scale (4) on described cylinder (7); The top of described cylinder (7) arranges dismountable U-shaped screen cloth (6), and described U-shaped screen cloth (6) is upper end open, the horn shape of lower end closed, and the sidewall of described U-shaped screen cloth (6) contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth (6), and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth (6); The bottom of described cylinder (7) is threaded connection and is connected with post tail (8), and the bottom of described post tail (8) is provided with liquid outlet (3), and the top of described liquid outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.2 ~ 0.4 μm;
The junction of the junction of described column cap (1) and cylinder (7), cylinder (7) and post tail (8) is provided with sealing ring, and described sealing ring is silica gel material;
The top of described sieve plate (5) arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643984.0A CN103691150B (en) | 2013-12-05 | 2013-12-05 | A kind of detachable chromatographic column |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643984.0A CN103691150B (en) | 2013-12-05 | 2013-12-05 | A kind of detachable chromatographic column |
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| Publication Number | Publication Date |
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| CN103691150A CN103691150A (en) | 2014-04-02 |
| CN103691150B true CN103691150B (en) | 2016-04-06 |
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| CN201310643984.0A Active CN103691150B (en) | 2013-12-05 | 2013-12-05 | A kind of detachable chromatographic column |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106267896A (en) * | 2015-06-03 | 2017-01-04 | 中美华世通生物医药科技（武汉）有限公司 | Isolated and purified device and isolation and purification method |
| CN105181922B (en) * | 2015-09-18 | 2017-04-19 | 上海昂林科学仪器有限公司 | Device for measuring animal and plant oil |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN201410310Y (en) * | 2009-03-17 | 2010-02-24 | 温超 | Chromatographic column with scales |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN203678013U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Splittable chromatographic column |
-
2013
- 2013-12-05 CN CN201310643984.0A patent/CN103691150B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN201410310Y (en) * | 2009-03-17 | 2010-02-24 | 温超 | Chromatographic column with scales |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| CN203678013U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Splittable chromatographic column |
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| CN103691150A (en) | 2014-04-02 |
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Denomination of invention: Detachable chromatographic column Effective date of registration: 20190425 Granted publication date: 20160406 Pledgee: Chengdu technical transformation incubator management Co., Ltd. Pledgor: Chengdu Yatu Biotechnology Co., Ltd. Registration number: 2019510000049 |
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Date of cancellation: 20200708 Granted publication date: 20160406 Pledgee: Chengdu technical transformation incubator management Co.,Ltd. Pledgor: YACHT BIOTECHNOLOGY Co. Registration number: 2019510000049 |