CN203678009U - Flow velocity controllable chromatographic column - Google Patents
Flow velocity controllable chromatographic column Download PDFInfo
- Publication number
- CN203678009U CN203678009U CN201320789866.6U CN201320789866U CN203678009U CN 203678009 U CN203678009 U CN 203678009U CN 201320789866 U CN201320789866 U CN 201320789866U CN 203678009 U CN203678009 U CN 203678009U
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- flow velocity
- cylinder
- chromatographic column
- screen cloth
- shaped screen
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Abstract
The utility model provides a flow velocity controllable chromatographic column, belonging to the field of biological purification appliances. The chromatographic column has the beneficial effects that a U-shaped screen is arranged at the top end of a column body, thus being convenient for liquids such as buffer solutions, sample solutions, eluants and the like to flow down from the four walls of the column body and avoiding causing splashing; a flow velocity controller is arranged at the lower part of a liquid outlet; the flow velocity can be controlled via the flow velocity controller and can be directly read out, thus being convenient for experiment operation; the flow velocity in chromatography operation is quantified, thus being beneficial for providing references for later purification operation of the same or similar proteins; the chromatographic column has a simple structure, is convenient to operate, and has a good protein purification effect.
Description
Technical field
The utility model belongs to biologically pure apparatus, is specifically related to the controlled chromatographic column of a kind of flow velocity.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, is mainly to adopt column chromatography, uses chromatographic column, realizes by column chromatography process.Traditional column chromatography is the top that mixing cpd is placed on to glass column post bed, make the eluant, eluent (mobile phase) of mixed solvent composition under Action of Gravity Field, by the vertical glass column of absorbent media material (fixing phase) is housed, separation obtains each purer product (sterling), its principle is: because each component in mixing cpd is to fixing different with the affinity of mobile phase mutually in chromatographic column, therefore select suitable fixing phase and mobile phase according to the character of mixing cpd, can reach the object that wherein each component separates as far as possible, the product separating is collected into respectively in each receiving flask in pillar outlet, then, identify the chromatographic solution of collecting in each receiving flask, merge and concentrated each identical chromatographic solution according to qualification result, obtain each sterling.
There is following problem in current chromatographic column: 1. the flow velocity that can not control liquid in the time carrying out chromatography operation, if the flow velocity of sample liquid is too fast, be easy to cause in destination protein and cylinder filler time of contact too short and should not be adsorbed, if the flow velocity of eluent is too fast, be also not easy destination protein exchange to wash; If flow velocity is excessively slow, can increase again the chromatography time, due to not being quantized of flow velocity, in the time processing same or similar albumen next time, cannot provide reference; 2. in the time of sample introduction, sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column.The filler of using due to chromatography is usually more expensive, and the filler splashing on inwall is not fully utilized in chromatography, affects chromatography effect.
Summary of the invention
For addressing the above problem, the utility model provides a kind of detachable chromatographic column, adopts following technical scheme:
The chromatographic column that flow velocity is controlled, comprises column cap and cylinder, and described column cap, is characterized in that for being threaded with cylinder, and the top of described column cap is provided with the through hole passing through for silica gel hose; The top of described cylinder arranges dismountable U-shaped screen cloth, and described U-shaped screen cloth is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth; The bottom of described cylinder is conical outlet; The top of described conical outlet arranges sieve plate, and the aperture of described sieve plate is 0.2 ~ 0.4 μ m; The bottom of described conical outlet arranges flow speed controller.
Preferably, the junction of described column cap and cylinder is provided with sealing ring, and described sealing ring is silica gel material.
The utlity model has following beneficial effect:
1. the chromatographic column that the utility model provides, dismountable U-shaped screen cloth is set at the top of cylinder, the horn shape that described U-shaped screen cloth is upper end open, lower end closed, and the sidewall of U-shaped screen cloth contains through hole, so setting, when being conducive to buffer solution, sample liquid, eluent and entering from cylinder top, be to flow down along the wall of cylinder, can not cause splashing of the interior liquid of cylinder, cause that filler splashes on the wall of cylinder, affect packed height, thereby affect the purification effect of albumen.
2. the chromatographic column that the utility model provides, in the bottom of cylinder, post tail is set, and on the inside of post tail, the top of liquid outlet, sieve plate is set, and the aperture that limits sieve plate is 0.2 ~ 0.4 μ m, and cellulose acetate film is set on sieve plate as optimal way, filler so so just can reach protein purification time not can along with buffer solution, sample liquid, eluent together, run off from conical outlet, but can not affect buffer solution, sample liquid and the eluent outflow effect from conical outlet; In addition, sieve plate is arranged on post tail, after purifying completes, can opens post tail, take out easily the filler in cylinder.
3. the chromatographic column that the utility model provides, middling speed qualitative filter paper filter membrane is placed on the top of U-shaped screen cloth, can carry out initial gross separation to sample liquid to be separated, the impurity such as the untreated thalline completely that interception size ratio is larger, can also avoid chromatography sample to stop up U-shaped screen cloth.
4. the chromatographic column that the utility model provides, is provided with sealing ring in the junction of column cap and cylinder, is convenient to the airtight of column cap and cylinder, avoids buffer solution, sample liquid, eluent to flow out from both gaps, causes waste.
5. the chromatographic column that the utility model provides, bottom at liquid outlet arranges flow speed controller, can control flow velocity by flow speed controller, and can directly read flow velocity, facilitate experimental implementation, flow velocity in chromatography operation is quantized, and the purification process being conducive to carry out identical or similar protein later provides reference role.
6. the chromatographic column that the utility model provides, simple in structure, easy to operate, the purification effect of albumen is good.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model; Wherein, 1 represents column cap; 2 represent silica gel hose; 3 represent conical outlet; 4 represent flow speed controller; 5 represent sieve plate; 6 represent U-shaped screen cloth; 7 represent cylinder.
The specific embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.Except following embodiment, the utility model can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of the utility model requirement.
As shown in Figure 1, the chromatographic column that a kind of flow velocity is controlled, comprises column cap 1 and cylinder 7, and described column cap 1, is characterized in that for being threaded with cylinder 7, and the top of described column cap 1 is provided with the through hole passing through for silica gel hose 2; The top of described cylinder 7 arranges dismountable U-shaped screen cloth 6, and described U-shaped screen cloth 6 is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth 6 contains through hole; Middling speed qualitative filter paper filter membrane (do not mark in the drawings, it is arranged on the top of screen cloth, but is no more than the horizontal level at cylinder top) is placed on the top of described U-shaped screen cloth 6, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth 6; The bottom of described cylinder 7 is conical outlet 3; The top of described conical outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.2 ~ 0.4 μ m; The bottom of described conical outlet 3 arranges flow speed controller 4.
As preferably, be provided with sealing ring (not marking out in the drawings) in the junction of column cap 1 and cylinder 7, for sealing function, described sealing ring is silica gel material, sealing effectiveness is better.
Claims (2)
1. the controlled chromatographic column of flow velocity, comprises column cap (1) and cylinder (7), and described column cap (1), is characterized in that for being threaded with cylinder (7), and the top of described column cap (1) is provided with the through hole passing through for silica gel hose (2); The top of described cylinder (7) arranges dismountable U-shaped screen cloth (6), and described U-shaped screen cloth (6) is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth (6) contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth (6), and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth (6); The bottom of described cylinder (7) is conical outlet (3); The top of described conical outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.2 ~ 0.4 μ m; The bottom of described conical outlet (3) arranges flow speed controller (4).
2. the controlled chromatographic column of flow velocity according to claim 1, is characterized in that, the junction of described column cap (1) and cylinder (7) is provided with sealing ring, and described sealing ring is silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320789866.6U CN203678009U (en) | 2013-12-05 | 2013-12-05 | Flow velocity controllable chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320789866.6U CN203678009U (en) | 2013-12-05 | 2013-12-05 | Flow velocity controllable chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203678009U true CN203678009U (en) | 2014-07-02 |
Family
ID=50999974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320789866.6U Withdrawn - After Issue CN203678009U (en) | 2013-12-05 | 2013-12-05 | Flow velocity controllable chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203678009U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691152A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Controllable-flow-rate chromatographic column |
-
2013
- 2013-12-05 CN CN201320789866.6U patent/CN203678009U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691152A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Controllable-flow-rate chromatographic column |
| CN103691152B (en) * | 2013-12-05 | 2016-04-06 | 成都雅途生物技术有限公司 | The chromatographic column that a kind of flow velocity is controlled |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140702 Effective date of abandoning: 20160406 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |