CN103691151B - Detachable antiskid chromatographic column - Google Patents
Detachable antiskid chromatographic column Download PDFInfo
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- CN103691151B CN103691151B CN201310643987.4A CN201310643987A CN103691151B CN 103691151 B CN103691151 B CN 103691151B CN 201310643987 A CN201310643987 A CN 201310643987A CN 103691151 B CN103691151 B CN 103691151B
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Abstract
The invention provides a kind of detachable antiskid chromatographic column, belong to biologically pure instrument field.Chromatographic column provided by the invention, cylinder top arranges U-shaped screen cloth, is convenient to the liquid such as buffer solution, sample liquid, eluent and flows down from the wall of cylinder, avoid causing splashing, affect chromatographic effect; Cartridge outer surface arranges anti-skidding plastic jacket, is convenient to hold cylinder or grip on the top of the shelf by cylinder, is not easily broken by chromatographic column, and avoids clip to leave cut on the cylinder of glass material.Chromatographic column provided by the invention, column cap and cylinder, cylinder and post tail all can split, and assembles before chromatography, after chromatography, can mutually take apart it, carry out respectively cleaning, the process such as sterilizing.Structure of the present invention is simple, and easy to operate, the purification effect of albumen is good.
Description
Technical field
The invention belongs to biologically pure apparatus, be specifically related to a kind of detachable antiskid chromatographic column.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, mainly adopts column chromatography, uses chromatographic column, is realized by column chromatography procedure.Traditional column chromatography is top mixing cpd being placed on glass column post bed, the eluant, eluent (mobile phase) that mixed solvent is formed under gravity, by being equipped with the vertical glass post of absorbent media material (Stationary liquid), be separated and obtain each purer product (sterling), its principle is: because component each in mixing cpd is different with the affinity of mobile phase to Stationary liquid in chromatographic column, therefore select suitable Stationary liquid and mobile phase according to the character of mixing cpd, the object wherein each component be separated as far as possible can be reached, the product be separated is collected in each receiving flask at column outlet respectively, then, identify the chromatographic solution collected in each receiving flask, merge and concentrated each identical chromatographic solution according to qualification result, obtain each sterling.
Common chromatographic column can be divided into normal pressure post, middle compression leg and high-pressure column according to pressure difference.Normal pressure post is with the most use in practice, is usually oneself to adopt the chromatographic column of different materials as required, load the separation and purification that different fillers carrys out realize target material.Glass chromatography column is especially conventional again, often comprises cylinder, piston.There is following problem in glass chromatography column common at present: the chromatographic column outer surface smoother of glass material, holding chromatographic column or when fixing chromatographic column with clip, all easily dropping, break chromatographic column, its inner precious filler trickled down.
In addition, current chromatographic column is when sample introduction, and sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column.Filler due to chromatography is usually costly, and the filler splashed on inwall is not fully utilized in chromatography, affects chromatographic effect.
Summary of the invention
For solving the problem, the invention provides a kind of antiskid chromatographic column, adopting following technical scheme:
A detachable antiskid chromatographic column, comprises column cap and cylinder, and described column cap and cylinder, for being threaded, is characterized in that, the top of described column cap is provided with the through hole passed through for plastic tube; Described cylinder adopts transparent glass material; The top of described cylinder arranges dismountable U-shaped screen cloth, and described U-shaped screen cloth is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth; The outer surface of described cylinder arranges anti-skidding plastic jacket; The bottom of described cylinder is threaded connection and is connected with post tail, and the bottom of described post tail arranges liquid outlet; Described liquid outlet comprises two mouths; The top of described liquid outlet arranges sieve plate, and the aperture of described sieve plate is for being less than or equal to 0.45 μm.
Preferably, the junction of described column cap and cylinder is provided with sealing ring, and described sealing ring is silica gel material.
Preferably, the top of described sieve plate arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder.
The present invention has following beneficial effect:
1. chromatographic column provided by the invention, arranges anti-skidding plastic jacket at the outer surface of cylinder, so arranges, and is convenient to hold cylinder or grip on the top of the shelf by cylinder, is not easily broken by chromatographic column, and avoids clip to leave cut on the cylinder of glass material; In addition, in order to avoid the observation of plastic jacket impact to cylinder content, transparent plastic jacket can be selected.
2. chromatographic column provided by the invention, at the top of cylinder, dismountable U-shaped screen cloth is set, described U-shaped screen cloth is the horn shape for upper end open, lower end closed, and the sidewall of U-shaped screen cloth contains through hole, so arranges, be conducive to buffer solution, sample liquid, eluent be when entering from cylinder top, be flow down along the wall of cylinder, the splashing of liquid in cylinder can not be caused, cause filler to splash on the wall of cylinder, affect packed height, thus affect the purification effect of albumen.
3. chromatographic column provided by the invention, on the top of liquid outlet, sieve plate is set, and the aperture limiting sieve plate is for being less than or equal to 0.45 μm, filler when so so just can reach protein purification can not along with buffer solution, sample liquid, eluent together, run off from conical outlet, but buffer solution, sample liquid and the eluent outflow effect from conical outlet can not be affected.
4. chromatographic column provided by the invention, middling speed qualitative filter paper filter membrane is placed on the top of U-shaped screen cloth, can carry out initial gross separation to sample liquid to be separated, and the impurity such as the untreated thalline completely that interception particle diameter is larger, can also avoid the blocking of U-shaped screen cloth.
5. chromatographic column provided by the invention, in the junction of column cap and cylinder, the junction of cylinder and post tail, be all provided with sealing ring, be convenient to the airtight of column cap and cylinder, cylinder and post tail, avoid buffer solution, sample liquid, eluent to flow out from both gaps, cause waste.
6. chromatographic column provided by the invention, liquid outlet comprises two mouths, one mouth is for connecting product reservoir, one mouth is for connecting waste fluid container, avoid the position changing product reservoir and waste fluid container when chromatography, thus increase work efficiency, especially product reservoir and waste fluid container all larger, and when being difficult to mobile, the advantage that this kind is arranged is more outstanding.
7. chromatographic column provided by the invention, column cap and cylinder, cylinder and post tail all can split, and assembles before chromatography, after chromatography, can mutually take apart it, carry out respectively cleaning, the process such as sterilizing.Structure of the present invention is simple, and easy to operate, the purification effect of albumen is good.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention; Wherein, 1 represents column cap; 2 represent plastic tube; 3 represent liquid outlet; 4 represent plastic jacket; 5 represent sieve plate; 6 represent U-shaped screen cloth; 7 represent cylinder; 8 represent post tail.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described.Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of application claims.
As shown in Figure 1, a kind of detachable antiskid chromatographic column, comprises column cap 1 and cylinder 7, and described column cap 1 and cylinder 7, for being threaded, is characterized in that, the top of described column cap 1 is provided with the through hole passed through for plastic tube 2; Described cylinder 7 adopts transparent glass material; The top of described cylinder 7 arranges dismountable U-shaped screen cloth 6, and described U-shaped screen cloth 6 is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth 6 contains through hole; Middling speed qualitative filter paper filter membrane (do not mark in the drawings, it is arranged on the top of screen cloth, but is no more than the horizontal level at cylinder top) is placed on the top of described U-shaped screen cloth 6, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth 6; The outer surface of described cylinder 7 arranges anti-skidding plastic jacket 4; The bottom of described cylinder 7 is threaded connection and is connected with post tail 8, and the bottom of described post tail 8 arranges liquid outlet 3; Described liquid outlet 3 comprises two mouths; The top of described liquid outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is for being less than or equal to 0.45 μm.
Preferably, the junction of the junction of described column cap 1 and cylinder 7, cylinder 7 and post tail 8 is provided with sealing ring (in the drawings not mark out), and for sealing function, described sealing ring is silica gel material, and sealing effectiveness is better.
Preferably, the top of described sieve plate 5 arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder 7.
Claims (1)
1. a detachable antiskid chromatographic column, comprises column cap (1) and cylinder (7), and described column cap (1) and cylinder (7), for being threaded, is characterized in that, the top of described column cap (1) is provided with the through hole passed through for plastic tube (2); Described cylinder (7) adopts transparent glass material; The top of described cylinder (7) arranges dismountable U-shaped screen cloth (6), and described U-shaped screen cloth (6) is upper end open, the horn shape of lower end closed, and the sidewall of described U-shaped screen cloth (6) contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth (6), and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth (6); The outer surface of described cylinder (7) arranges anti-skidding plastic jacket (4); The bottom of described cylinder (7) is threaded connection and is connected with post tail (8), and the bottom of described post tail (8) arranges liquid outlet (3); Described liquid outlet (3) comprises two mouths; The top of described liquid outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is for being less than or equal to 0.45 μm;
The top of sieve plate (5) arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder (7);
The junction of described column cap (1) and cylinder (7) is provided with sealing ring, and described sealing ring is silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643987.4A CN103691151B (en) | 2013-12-05 | 2013-12-05 | Detachable antiskid chromatographic column |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643987.4A CN103691151B (en) | 2013-12-05 | 2013-12-05 | Detachable antiskid chromatographic column |
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| Publication Number | Publication Date |
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| CN103691151A CN103691151A (en) | 2014-04-02 |
| CN103691151B true CN103691151B (en) | 2016-04-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310643987.4A Active CN103691151B (en) | 2013-12-05 | 2013-12-05 | Detachable antiskid chromatographic column |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106267891B (en) * | 2015-06-03 | 2023-06-06 | 中美华世通生物医药科技(武汉)股份有限公司 | Method for rapid separation and purification of compound |
| CN106669230A (en) * | 2016-11-25 | 2017-05-17 | 南京海獭软件有限责任公司 | High-efficiency pressurized chromatographic column used in laboratory |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| CN202700140U (en) * | 2012-07-23 | 2013-01-30 | 常州三泰科技有限公司 | Liquid chromatography column |
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN203678014U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Splittable antiskid chromatographic column |
-
2013
- 2013-12-05 CN CN201310643987.4A patent/CN103691151B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8506804B1 (en) * | 2004-05-10 | 2013-08-13 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN202700138U (en) * | 2012-06-19 | 2013-01-30 | 石家庄诚志永华显示材料有限公司 | Split-type chromatographic column device |
| CN202700140U (en) * | 2012-07-23 | 2013-01-30 | 常州三泰科技有限公司 | Liquid chromatography column |
| CN203678014U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Splittable antiskid chromatographic column |
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| CN103691151A (en) | 2014-04-02 |
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