CN203678012U - Splittable chromatographic column - Google Patents
Splittable chromatographic column Download PDFInfo
- Publication number
- CN203678012U CN203678012U CN201320789915.6U CN201320789915U CN203678012U CN 203678012 U CN203678012 U CN 203678012U CN 201320789915 U CN201320789915 U CN 201320789915U CN 203678012 U CN203678012 U CN 203678012U
- Authority
- CN
- China
- Prior art keywords
- cylinder
- column
- chromatographic column
- sieve plate
- column body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 229920003023 plastic Polymers 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000741 silica gel Substances 0.000 claims description 12
- 229910002027 silica gel Inorganic materials 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 9
- 229920002301 cellulose acetate Polymers 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000746 purification Methods 0.000 abstract description 5
- 238000001742 protein purification Methods 0.000 abstract description 2
- 229920001296 polysiloxane Polymers 0.000 abstract 2
- 239000000945 filler Substances 0.000 description 14
- 238000004587 chromatography analysis Methods 0.000 description 12
- 239000000523 sample Substances 0.000 description 10
- 239000003480 eluent Substances 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 238000000926 separation method Methods 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000004440 column chromatography Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model provides a splittable chromatographic column, belonging to the field of biological purification appliances. The chromatographic column comprises a column head and a column body, wherein the column head and the column body are in threaded connection; a through hole for a plastic pipe to pass through is formed in the top of the column head; the column body is made of transparent plastic materials; uniform scales are arranged on the column body along the axial direction of the column body; the plastic pipe extends into the bottom in the column body and is connected with a four-way pipe; the other three ends of the four-way pipe are respectively connected with a silicone hose; the other ends of the silicone hoses come into contact with the inner walls of the column body; the bottom of the column body is connected with a column tail by a thread; a liquid outlet is formed in the bottom of the column tail; a sieve plate is arranged at the upper part of the liquid outlet; the aperture of the sieve plate is 0.3-0.45mu m. The splittable chromatographic column has a simple structure, is convenient to operate, and has a good protein purification effect.
Description
Technical field
The utility model belongs to biologically pure apparatus, is specifically related to a kind of detachable chromatographic column.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, is mainly to adopt column chromatography, uses chromatographic column, realizes by column chromatography process.Traditional column chromatography is the top that mixing cpd is placed on to glass column post bed, make the eluant, eluent (mobile phase) of mixed solvent composition under Action of Gravity Field, by the vertical glass column of absorbent media material (fixing phase) is housed, separation obtains each purer product (sterling), its principle is: because each component in mixing cpd is to fixing different with the affinity of mobile phase mutually in chromatographic column, therefore select suitable fixing phase and mobile phase according to the character of mixing cpd, can reach the object that wherein each component separates as far as possible, the product separating is collected into respectively in each receiving flask in pillar outlet, then, identify the chromatographic solution of collecting in each receiving flask, merge and concentrated each identical chromatographic solution according to qualification result, obtain each sterling.
Common chromatographic column can be divided into normal pressure post, middle compression leg and high-pressure column according to pressure difference.Normal pressure post is with the most use in practice, is usually the chromatographic column that oneself adopts as required different materials, the separation and purification of loading different fillers and come realize target material.Glass chromatography column is especially conventional again, often comprises cylinder, piston, is positioned at also comprising of having the barb for fixing liquid feeding ball of cylinder upper end etc.At present common glass chromatography column exists following not enough: on the one hand, in the filler filling and the filling of separated object quality sample of glass chromatography column, usually adopt different fillers according to different fillers and different material, sample ratio, be beneficial to save filler in reaching optimal separation effect, and filler. the ratio of sample is conventionally with reference to its volume ratio, but at present conventional glass column does not have scale, usually need operating personnel to estimate by rule of thumb or measure with other measurement facilities, bothering very much; On the other hand, in chromatography process, usually needing to be grasped sample is the migration velocity in filler at separating medium, then carry out the speed adjusting of efflux according to migration velocity, be beneficial to save the chromatography time in reaching optimal separation effect, but current chromatographic column does not have scale, usually to the position of certain chromatography colour band marking pen mark in different time points, such way is not accurate enough, the leakage that simultaneously mark of marker pen institute mark may be when adding eluant, eluent and being washed, need mark again, very inconvenient.
In addition, current chromatographic column is in the time of sample introduction, and sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column.The filler of using due to chromatography is usually more expensive, and the filler splashing on inwall is not fully utilized in chromatography, affects chromatography effect.
Summary of the invention
For addressing the above problem, the utility model provides a kind of detachable chromatographic column, adopts following technical scheme:
A detachable chromatographic column, comprises column cap and cylinder, and described column cap, is characterized in that for being threaded with cylinder, and the top of described column cap is provided with the through hole passing through for plastic tube; Described cylinder adopts transparent plastic material, on described cylinder along cylinder axis to being provided with uniform scale; The bottom that described plastic tube stretches in cylinder connects a four-way pipe, and other three ends of described four-way pipe connect respectively a silica gel hose, and the other end of described silica gel hose contacts with the inwall of cylinder; The bottom of described cylinder is connected with post tail by screw thread, and the bottom of described post tail arranges liquid outlet, and the top of described liquid outlet arranges sieve plate, and the aperture of described sieve plate is 0.3 ~ 0.45 μ m.
Preferably, the junction of the junction of described column cap and cylinder, cylinder and post tail is provided with sealing ring, and described sealing ring is silica gel material.
Preferably, the top of described sieve plate arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder.
The utlity model has following beneficial effect:
1. the chromatographic column that the utility model provides, its cylinder adopts transparent plastic material, and uniform scale is set on cylinder, so arrange, be conducive to easily and accurately hold packing samples ratio in the time of the filler filling of chromatographic column and sample loading, by observing the scale position of certain colour band at different time points place, thus the migration rate of the separated material of convenient grasp in chromatographic column, make whole chromatography process more convenient, improve separative efficiency and whole operating efficiency.
2. the chromatographic column that the utility model provides, bottom in plastic tube stretches into cylinder connects a four-way pipe, other three ends of described four-way pipe connect respectively a silica gel hose, the other end of described silica gel hose contacts with the inwall of cylinder, so arrange, when being conducive to buffer solution, sample liquid, eluent and entering from cylinder top, to flow down along the sidewall of cylinder, can not cause splashing of the interior liquid of cylinder, cause that filler splashes on the sidewall of cylinder, affect packed height, thereby affect the purification effect of albumen.
3. the chromatographic column that the utility model provides, in the bottom of cylinder, post tail is set, and on the inside of post tail, the top of its through hole, sieve plate is set, and the aperture that limits sieve plate is 0.3 ~ 0.45 μ m, and cellulose acetate film is set on sieve plate as optimal way, filler so so just can reach protein purification time not can along with buffer solution, sample liquid, eluent together, run off from conical outlet, but can not affect buffer solution, sample liquid and the eluent outflow effect from conical outlet; In addition, sieve plate is arranged on post tail, after purifying completes, can opens post tail, take out easily the filler in cylinder.
4. the chromatographic column that the utility model provides, in the junction of junction, cylinder and the post tail of column cap and cylinder, be all provided with sealing ring, be convenient to the airtight of column cap and cylinder, cylinder and post tail, avoid buffer solution, sample liquid, eluent to flow out from both gaps, cause waste.
5. the chromatographic column that the utility model provides, column cap and cylinder, cylinder and post tail all can split, and it are assembled before chromatography, after chromatography, can mutually take apart, clean respectively, the processing such as sterilizing.The utility model is simple in structure, easy to operate, and the purification effect of albumen is good.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model; Wherein, 1 represents column cap; 2 represent plastic tube; 3 represent liquid outlet; 4 represent scale; 5 represent sieve plate; 6 represent silica gel hose; 7 represent cylinder; 8 represent post tail.
Detailed description of the invention
Below in conjunction with drawings and Examples, the utility model is described in further detail.Except following embodiment, the utility model can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of the utility model requirement.
As shown in Figure 1,
A detachable chromatographic column, comprises column cap 1 and cylinder 7, and described column cap 1, is characterized in that for being threaded with cylinder 7, and the top of described column cap 1 is provided with the through hole passing through for plastic tube 2; Described cylinder 7 adopts transparent plastic material, on described cylinder 7 along cylinder axis to being provided with uniform scale 4; The bottom that described plastic tube 2 stretches in cylinder 7 connects a four-way pipe, and other three ends of described four-way pipe connect respectively a silica gel hose 6, and the other end of described silica gel hose 6 contacts with the inwall of cylinder 7; The bottom of described cylinder 7 is connected with post tail 8 by screw thread, and the bottom of described post tail 8 arranges liquid outlet 3, and the top of described liquid outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.3 ~ 0.45 μ m.
Preferably, the junction of the junction of described column cap 1 and cylinder 7, cylinder 7 and post tail 8 is provided with sealing ring, and (not marking out in the drawings), for sealing function, described sealing ring is silica gel material, sealing effectiveness is better.
Preferably, the top of described sieve plate 5 arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder 7.
Claims (3)
1. a detachable chromatographic column, comprises column cap (1) and cylinder (7), and described column cap (1), is characterized in that for being threaded with cylinder (7), and the top of described column cap (1) is provided with the through hole passing through for plastic tube (2); Described cylinder (7) adopts transparent plastic material, and described cylinder (7) is upper along cylinder axis to being provided with uniform scale (4); The bottom that described plastic tube (2) stretches in cylinder (7) connects a four-way pipe, and other three ends of described four-way pipe connect respectively a silica gel hose (6), and the other end of described silica gel hose (6) contacts with the inwall of cylinder (7); The bottom of described cylinder (7) is connected with post tail (8) by screw thread, and the bottom of described post tail (8) arranges liquid outlet (3), and the top of described liquid outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.3 ~ 0.45 μ m.
2. detachable chromatographic column according to claim 1, is characterized in that, the junction of junction, cylinder (7) and the post tail (8) of described column cap (1) and cylinder (7) is provided with sealing ring, and described sealing ring is silica gel material.
3. detachable chromatographic column according to claim 1, is characterized in that, the top of described sieve plate (5) arranges cellulose acetate film, and the diameter of described cellulose acetate film is not less than the diameter of cylinder (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320789915.6U CN203678012U (en) | 2013-12-05 | 2013-12-05 | Splittable chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320789915.6U CN203678012U (en) | 2013-12-05 | 2013-12-05 | Splittable chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203678012U true CN203678012U (en) | 2014-07-02 |
Family
ID=50999977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320789915.6U Expired - Lifetime CN203678012U (en) | 2013-12-05 | 2013-12-05 | Splittable chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203678012U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691155A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Detachable chromatographic column |
| CN105403646A (en) * | 2015-11-02 | 2016-03-16 | 刘泰峰 | Embedded type chromatographic column capable of randomly cutting color tape |
| CN106390520B (en) * | 2016-11-18 | 2019-01-25 | 中国石油大学(北京) | The separation method of Various Groups In Crude Oil analysis chromatographic column and Various Groups In Crude Oil |
-
2013
- 2013-12-05 CN CN201320789915.6U patent/CN203678012U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691155A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Detachable chromatographic column |
| CN105403646A (en) * | 2015-11-02 | 2016-03-16 | 刘泰峰 | Embedded type chromatographic column capable of randomly cutting color tape |
| CN106390520B (en) * | 2016-11-18 | 2019-01-25 | 中国石油大学(北京) | The separation method of Various Groups In Crude Oil analysis chromatographic column and Various Groups In Crude Oil |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140702 |
|
| CX01 | Expiry of patent term |