CN103690771A - Drug for treating colibacillosis in lambs and preparation method of drug - Google Patents

Abstract

The invention discloses a drug for treating the colibacillosis in lambs and a preparation method of the drug. The drug comprises the following raw medicinal materials: rhizoma coptidis, Chinese pulsatilla root, diversifolious buckthorn root or leaf, dried tangerine or orange peel, radix paeoniae rubra, radix angelicae sinensis, semen cassia, garlic, rhodiola rosea, areca catechu, honeysuckle, ash bark, cassia bark, carbonized sanguisorba root, fructus aurantii immaturus, smartweed, coriander seed, hawthorn, the root of kudzu vine, radix ophiopogonis, portulaca oleracea, liquorice and crispy rice. The drug has the benefits that based on the principle of clearing heat, sterilizing, relieving internal heat or fever, cooling blood, and reinforcing and protecting the stomach-qi, when being used for treating the colibacillosis in lambs, the drug has the advantages of quick effect, low possibility of generating drug resistance, low possibility of generating drug resistance and enabling colibacillosis in lambs to recurrence, small side effects, low cost, and small animal product drug residue amount, and is beneficial to human and animal safety.

Description

Be used for the treatment of medicine of lamb colibacillosis and preparation method thereof

Technical field

The present invention relates to herbal medicine technical field, particularly a kind of medicine that is used for the treatment of lamb colibacillosis and preparation method thereof.

Background technology

Greatly developing farmer breed sheep, increase farmers' income, is the Important Action of developing animal husbandry.But, along with rolling up of sheep quantity, and due to the impact of the objective factors such as natural environment, geographical conditions, anthropic factor, sheep colibacillosis is happened occasionally.

Lamb colibacillosis, is the disease being caused by the different escherichia coli of some serotypes, and mortality rate is very high.Mainly to pass through digestive tract infection.Lamb, contact disease sheep, antihygienic environment, while sucking the sordid nipple of ewe, all can infect.Small part is by intrauterine infection or through the skin infection of umbilical cord and damage.The lamb that sheep colibacillosis is multiple is born in age week a few days to 6, be endemicity popular, also has to distribute.Weather is bad, undernutrition, place are moist filthy etc., easily causes morbidity; Mainly during the drylot feeding of Winter-Spring, occur; Through digestive tract infection.

Escherichia coli are Gram-negative, medium sized bacillus.In classification, belong to enterobacteriaceae, Escherichia.Without brood cell, there is all flagellums, strong to carbohydrate fermentability.This bacterium to external world resistance of unfavorable factor is not strong, 60 ℃ 15 minutes dead, general common disinfectants is all easily killed.The non-pathogenic escherichia coli that normally live away from home in Escherichia coli and animal intestinal do not have difference at aspects such as form, dyeing, cultural character and biochemical reactions, but antigenic structure is different.Pathogenic bacterial strains generally can produce a kind of endotoxin and 1-2 kind enterotoxin.The high heat of endotoxin ability, 100 ℃ of 30 minutes ability are destroyed.Enterotoxin has two kinds, and a kind of thermo-labile (LT), has antigenicity, and molecular weight is large, and 60 ℃ destroyed through 10 minutes; Another kind of heat-resisting (ST), no antigen, molecular weight is little, must 60 ℃ above and long period could be destroyed.Escherichia coli have somatic antigen (O), surface antigen (K) and 3 kinds of major antigens of flagellar antigen (H), and in addition, many pathogenic strains relevant with diarrhoea are with fimbrial antigen (being also the plain antigen of adhesion or colonization factor antigen).According to antigenic component, Escherichia coli is divided into much serotype, cause a kind of escherichia coli of animal morbidity, be often certain serotype, a drove is as can't help other places introduction domestic animal of the same race, and its pathogenicity bacterial strain is often certain 1-2 kind serotype.

Incubation period 1-2 days.Be divided into septic and dysentery type amphitypy.(1) septic.Multiplely be born in lamb in age in 2-6 week.Sick sheep body temperature 41-42 ℃, spirit is depressed, collapse, has slight diarrhoea or does not suffer from diarrhoea rapidly, have with nervous symptoms, fortune step is lacked of proper care, is ground one's teeth in sleep, visual disorder, there is arthritis in the case also having, more than death in 4-12 hour after being ill.(2) dysentery type.Multiplely be born in the newborn lamb of 2-8 age in days.Sick just body temperature is slightly high, occurs temperature decline after diarrhoea, and it is half aqueous that feces is, and with bubble, is sometimes mixed with blood.Lamb performance stomachache, weakness, serious dehydration, can not stand up.As treatment not in time, can be in death in 24-36 hour, case fatality rate 15-17%.

Western medicine particularly oxytetracycline, sulfonamides and nitrofurans treatment lamb colibacillosis disease has easy generation drug resistance, suppresses gastrointestinal beneficial microbe, upsets balance between rumen microbial population, upsets Rumen Internal Environment, causes serious superinfection and the obstinate side effect such as dyspepsia, have in livestock products simultaneously residual quantity high, threaten human food's shortcoming such as safely.In clinical practice, also often see due to a large amount of life-time service antibiotics, particularly antibiotics for oral administration, causes serious dyspepsia and rumen peristalsis to weaken even and stops, and appetite, ruminates completely absolutely uselessly, is difficult to the case of rehabilitation again.

Chinese veterinarian is dialectical to be thought, the sick position of lamb colibacillosis, at taste, relates to the heart, liver, kidney, large intestine, small intestinal etc.Row epidemic disease poison during diseases caused by exogenous pathogenic factor, internal injury diet and cause heresy and accumulate intestinal internal organs, stagnation of QI-blood, conduction mistake department, all can cause the generation of primary disease.< < doctor rock dysentery > > says: " no matter what internal organs is damp and hot, all people's the intestines and stomach, take stomach as middle soil, lead the intestinal of holding and passing also." along with the evolution of disease, epidemic disease poison is contained very much also can involve the heart, liver, protracted inflammation, also can be thoroughly and in kidney.With respect to Western medicine such as antibiotic such as oxytetracycline, sulfonamides and furans, treatment by Chinese herbs have instant effect, curative effect really, be difficult for developing immunity to drugs, cheap, be difficult for recurrence, few side effects, livestock products drug residue low, be conducive to the advantages such as person poultry safety.Therefore select treatment by Chinese herbs lamb colibacillosis disease necessary.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of medicine that is used for the treatment of lamb colibacillosis, adopt this medicine thering is instant effect aspect treatment lamb colibacillosis, be difficult for developing immunity to drugs, cheap, be difficult for recurrence, side effect is little, livestock products drug residue is low and be conducive to person poultry safety a little.

In order to realize foregoing invention object, the invention provides a kind of medicine that is used for the treatment of lamb colibacillosis, it is characterized in that, described medicine comprises following raw medicinal material: Rhizoma Coptidis, the Radix Pulsatillae, Radix Rhamni Heterophyllae, Pericarpium Citri Reticulatae, Radix Paeoniae Rubra, Radix Angelicae Sinensis, Semen Cassiae, Bulbus Allii, Radix Rhodiolae, Semen Arecae, Flos Lonicerae, Cortex Fraxini, Cortex cinnamomi japonici (Ramulus Cinnamomi), charred Radix Sanguisorbae, Fructus Aurantii Immaturus, Herba Polygoni cymosi, Fructus Coriandri, Fructus Crataegi, Radix Puerariae, Radix Ophiopogonis, Herba Portulacae, Radix Glycyrrhizae and pot cake.

Described medicine is comprised of the raw medicinal material of following parts by weight: Rhizoma Coptidis 10-30 weight portion, Radix Pulsatillae 10-20 weight portion, Radix Rhamni Heterophyllae 10-20 weight portion, Pericarpium Citri Reticulatae 10-20 weight portion, Radix Paeoniae Rubra 15-25 weight portion, Radix Angelicae Sinensis 10-20 weight portion, Semen Cassiae 10-15 weight portion, Bulbus Allii 10-15 weight portion, Radix Rhodiolae 10-20 weight portion, Semen Arecae 10-15 weight portion, Flos Lonicerae 10-15 weight portion, Cortex Fraxini 10-15 weight portion, Cortex cinnamomi japonici (Ramulus Cinnamomi) 15-20 weight portion, charred Radix Sanguisorbae 10-20 weight portion, Fructus Aurantii Immaturus 10-20 weight portion, Herba Polygoni cymosi 10-20 weight portion, Fructus Coriandri 10-20 weight portion, Fructus Crataegi 10-20 weight portion, Radix Puerariae 10-20 weight portion, Radix Ophiopogonis 10-20 weight portion, Herba Portulacae 10-20 weight portion, Radix Glycyrrhizae 10-20 weight portion and pot cake 10-20 weight portion.

Described medicine is comprised of the raw medicinal material of following parts by weight: Rhizoma Coptidis 15-25 weight portion, Radix Pulsatillae 13-17 weight portion, Radix Rhamni Heterophyllae 12-18 weight portion, Pericarpium Citri Reticulatae 13-18 weight portion, Radix Paeoniae Rubra 18-23 weight portion, Radix Angelicae Sinensis 13-16 weight portion, Semen Cassiae 12-15 weight portion, Bulbus Allii 10-13 weight portion, Radix Rhodiolae 13-17 weight portion, Semen Arecae 12-15 weight portion, Flos Lonicerae 10-13 weight portion, Cortex Fraxini 11-14 weight portion, Cortex cinnamomi japonici (Ramulus Cinnamomi) 16-19 weight portion, charred Radix Sanguisorbae 13-17 weight portion, Fructus Aurantii Immaturus 13-17 weight portion, Herba Polygoni cymosi 12-18 weight portion, Fructus Coriandri 13-17 weight portion, Fructus Crataegi 14-17 weight portion, Radix Puerariae 13-17 weight portion, Radix Ophiopogonis 12-17 weight portion, Herba Portulacae 12-16 weight portion, Radix Glycyrrhizae 15-20 weight portion and pot cake 10-17 weight portion.

Described medicine is comprised of the raw medicinal material of following parts by weight: Rhizoma Coptidis 23 weight portions, the Radix Pulsatillae 15 weight portions, Radix Rhamni Heterophyllae 15 weight portions, Pericarpium Citri Reticulatae 16 weight portions, Radix Paeoniae Rubra 21 weight portions, Radix Angelicae Sinensis 14 weight portions, Semen Cassiae 13 weight portions, Bulbus Allii 12 weight portions, Radix Rhodiolae 15 weight portions, Semen Arecae 13 weight portions, Flos Lonicerae 12 weight portions, Cortex Fraxini 12 weight portions, Cortex cinnamomi japonici (Ramulus Cinnamomi) 17 weight portions, charred Radix Sanguisorbae 15 weight portions, Fructus Aurantii Immaturus 15 weight portions, Herba Polygoni cymosi 15 weight portions, Fructus Coriandri 16 weight portions, Fructus Crataegi 16 weight portions, Radix Puerariae 15 weight portions, Radix Ophiopogonis 14 weight portion, Herba Portulacae 14 weight portions, Radix Glycyrrhizae 18 weight portions and pot cake 13 weight portions.

In order better to realize foregoing invention object, the present invention also provides a kind of preparation method that is used for the treatment of the medicine of lamb colibacillosis, and the dosage form of described medicine is powder, and described preparation method comprises the following steps:

All raw medicinal materials are mixed in proportion, are ground into 100 order～200 object powder, add the water with respect to 3～5 times of mixture qualities, at the temperature of 90 ℃～100 ℃, decoct 30 minutes～1 hour, decoction liquor is filtered, and obtains the first filtrate and filtering residue;

Second step, the filtering residue that the first step is obtained adds the water with respect to 1～3 times of first step mixture quality, at the temperature of 90 ℃～100 ℃, decocts 30 minutes～1 hour, and decoction liquor is filtered, and obtains the second filtrate;

The 3rd step, merges the first filtrate and the second filtrate, and concentrating under reduced pressure is except desolventizing, obtain and get dry extract, dry cream is put into spray dryer dry, guarantee that inlet temperature is at 170 ℃～190 ℃, 60 ℃～80 ℃ of leaving air temps, are ground into 200 order～400 orders subsequently, obtain drug powder.

The present invention also provides a kind of preparation method that is used for the treatment of the medicine of lamb colibacillosis, and the dosage form of described medicine is injection, and described preparation method comprises the following steps:

The first step, is mixed in proportion pulverizing by all raw medicinal materials, decocts with water 2～4 times, each decocting time is 2 hours～3 hours, the water quality adding is 2～4 times of first step mixture quality, merges decoction liquor, filters, filtrate is concentrated into syrupy shape, adding determining alcohol is more than 95% ethanol, and the ethanol content adding is 1～2 times of mixture quality of the first step, after standing 12 hours～24 hours, centrifugal filtration, filtrate is adjusted to filtrate pH7～8 with 40%～50% NaOH;

Second step, the filtrate that the first step is obtained, adds 0.5%～1% active carbon and boils 18～22 minutes, filters, decarburization, injects water, and the crude drug concentration that makes injection is 2g/ml～4g/ml, fill sterilizing 1.5 hours～2 hours in 500ml saline bottle, after freezing a couple of days, filters.

The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is: medicine of the present invention take heat clearing away sterilization, removing toxic substances removing heat from blood, turn round and look at and protect gastric qi as principle, in treatment lamb colibacillosis, there is instant effect, be difficult for developing immunity to drugs, few side effects, be difficult for recurrence, cheap, livestock products drug residue is low and be conducive to person poultry safety.

The specific embodiment

Chinese veterinarian thinks that lamb colibacillosis pathological factor has wet, hot (or cold), poison, food etc., and the heresy of epidemic disease caused by damp-heat pathogen poison is many, and the heresy of cold-damp is less.Sick position is at intestinal internal organs, and relevant with taste, this is because heresy is from mouth people, through stomach spleen and stagnant in intestinal therefore.Therefore < < doctor rock dysentery > > says: " no matter what internal organs is damp and hot, all people's the intestines and stomach, take stomach as middle soil, lead the intestinal of holding and passing also." along with the evolution of disease, epidemic disease poison is contained very much also can involve the heart, liver, protracted inflammation, also can be thoroughly and in kidney ".In addition, seasonal pathogen epidemic disease poison stagnates between intestinal, stops up stagnant QI and blood, hinders conduction, intestinal adipose membrane injury of small vessels, and corruption turns to pus and blood and forms dysentery.The duty of intestinal department conduction, transmits waste matter, the further absorption of main body fluid again, and wet, heat, epidemic disease are malicious etc., and pathogenic factor stagnates in large intestine, so that the retardance of intestinal internal organs mechanism of qi, and body fluid is malabsorption again, and intestinal can not normal conduction waste matter, thereby produces stomachache, the not normal disease of defecating.Therapeutic Principle protects gastric qi for heat clearing away sterilization, removing toxic substances removing heat from blood, eliminating evil intestinal stasis relieving, QI and blood regulating, Gu.

Based on above-mentioned theory of Chinese medical science principle, the invention provides a kind of medicine that is used for the treatment of lamb colibacillosis, comprise following raw medicinal material: Rhizoma Coptidis, the Radix Pulsatillae, Radix Rhamni Heterophyllae, Pericarpium Citri Reticulatae, Radix Paeoniae Rubra, Radix Angelicae Sinensis, Semen Cassiae, Bulbus Allii, Radix Rhodiolae, Semen Arecae, Flos Lonicerae, Cortex Fraxini, Cortex cinnamomi japonici (Ramulus Cinnamomi), charred Radix Sanguisorbae, Fructus Aurantii Immaturus, Herba Polygoni cymosi, Fructus Coriandri, Fructus Crataegi, Radix Puerariae, Radix Ophiopogonis, Herba Portulacae, Radix Glycyrrhizae and pot cake.

Wherein, the pharmacological property of each raw medicinal material is:

Rhizoma Coptidis: heat clearing and damp drying, eliminating fire and detoxication.For damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion, toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.The kind clear part of the body cavity above the diaphragm housing the heart and lungs of Rhizoma Coptidis (processed with wine) is burning hot.For conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.For cold and heat, tie mutually damp and hot middle resistance, feeling of fullness vomiting.Cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.For incoordination between the liver and stomach, vomiting acid regurgitation.

The Radix Pulsatillae: bitter in the mouth, cold in nature; Return stomach, large intestine channel; There is heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, the effect of removing dampness and killing parasites; Cure mainly pyretic toxicity dysentery, epistaxis, internal hemorrhoid with bleeding, leukorrhagia, pudendal pruritus, carbuncle skin ulcer, scrofula antibacterial action, the fresh juice of the Radix Pulsatillae, decoct, ethanol extraction equal the external obvious antibacterial action that all has, and can suppress the growth of staphylococcus aureus, bacillus pyocyaneus, dysentery bacterium, bacillus subtilis, Bacillus typhi, salmonella etc.To diphtheria corynebacterium, staphylococcus, streptococcus, escherichia coli, tubercule bacillus effect in addition, the Radix Pulsatillae and compound recipe thereof all have inhibitory action to dermatophytes, yeast, trypanosomicide, Candida albicans etc. as Radix Pulsatillae Decoction to Anemonin.The Radix Pulsatillae have stronger killing fungus effect.The Radix Pulsatillae also has slight inhibitory action to white mice influenza infection, and its infusion can extend the time-to-live of influenza virus infection PR8 strain mice, alleviates pneumonia.Radix Pulsatillae ethanol extraction has calmness, analgesic activity.

Radix Rhamni Heterophyllae: cool in nature, the micro-hardship of puckery, nontoxic.Function cures mainly: heat clearing away, removing heat from blood, hemostasis.Control haematemesis, hemorrhoidal bleeding, metrorrhagia, menoxenia, dysentery.1. < < Guizhou folk medicine > >: " heat clearing away, removing heat from blood." 2. < < in Nanchuan commonly uses Chinese herbal medicine handbook > >: " smash floss and applied sore." 3. Chinese herbal medicine > > is commonly used in < < Sichuan: " heat clearing away removing food stagnancy, promoting blood circulation to restore menstrual flow.Control food stagnation, Liver Channel is stagnant, and menoxenia is spitted blood, impairment caused by overstrain, cough, pain due to disorder of QI."

Pericarpium Citri Reticulatae: warm in nature, acrid in the mouth, hardship; Return spleen, lung meridian, for diseases such as distension and fullness of the chest and abdomen.The pungent loose logical temperature of Pericarpium Citri tangerinae, fragrant odour, is longer than and regulates the flow of vital energy, can enter spleen lung, thus can go loose sluggishness of lung-QI, again can promoting the circulation of QI to alleviate the stagnation in middle-JIAO, for lung qi, gather around the diseases such as stagnant, chest and diaphragm feeling of fullness and stagnation of QI in spleen and stomach, distension and fullness in the abdomen, for retention of dampness in middle-JIAO, gastral cavity abdomen painful abdominal mass is swollen, have loose bowels in loose stool, and the disease such as abundant expectoration cough.Pericarpium Citri tangerinae dispelling the damp pathogen with bitter and warm drugs and can spleen invigorating circulation of qi promoting, therefore be usually used in the diseases such as retention of dampness in middle-JIAO, gastral cavity abdominal distention are vexed, loose stool greasy fur, can compatibility Rhizoma Atractylodis, Cortex Magnoliae Officinalis is with using.Be good at again drying dampness to eliminate phlegm, for controlling damp-phlegm, stop up the conventional key medicine of lung, abundant expectoration cough, for insufficiency of the spleen diet reduce, dyspepsia, and the disease such as nausea and vomiting.This product dampness and can spleen benefiting and stimulating the appetite, is applicable to the diseases such as weakness of the spleen and stomach, diet minimizing, dyspepsia, loose stool and diarrhea, the fit applications such as normal and Radix Ginseng, the Rhizoma Atractylodis Macrocephalae, Poria.Can spleen invigorating because of it, can regulate the flow of vital energy again, therefore often make as the assistant of Qi-tonifying drug, can make tonify without causing stagnation, prevent from stopping up and hold back the swollen effect of doing, in addition, Pericarpium Citri tangerinae again can with in, can control stomach-QI being unable to descend normally, nausea and vomiting.

Radix Paeoniae Rubra: hardship, be slightly cold, return Liver Channel.Clearing away heat and cooling blood, eliminating stasis to stop pain.For maculae caused by violent heat pathogen, hematemesis and epistaxis, conjunctival congestion and swelling pain, hypochondriac pain due to stagnation of liverQI, amenorrhea dysmenorrhea, lump in the abdomen stomachache, injury from falling down, carbuncle skin infection.< < herbal classic > >: main pathogen stomachache, except arthralgia due to stagnation of blood, broken hard long-pending, cold and heat hernia abdominal mass, pain relieving, diuresis, QI invigorating.< < property of medicine opinion > >: control pathogen of the lung gas Fu Zhong Xu pain, vim and vigour are gathered, logical a surname's internal organs are gathered around gas, control evil pain deteriorated blood, disease bone heat when main, strong the five internal organs, the kidney invigorating gas, control trusted subordinate's heavily fortified point swollen, married woman's blood closes obstructed, and repercussive blood can lose pus.< < does not record > >: clear and coherent blood vessels, slow in, dispelling malignant blood, expelling extravasated blood, the gas that anhydrates, sharp bladder intestine and small intestine, subduing inflammation, time row cold and heat, attacked by pestiferous factors stomachache, lumbago.

Radix Angelicae Sinensis: sweet in the mouth and weighing, therefore specially can enrich blood, its gas is light and pungent, thus again can promoting the circulation of blood, invigorating middle warmer has, in row, has benefit, really the gas medicine in blood, the also panacea in blood.About mending to mend of assistant, thus can support nutrition blood, QI invigorating spermatogenesis, settling five organs, body by force, beneficial mind, all tangible deficient diseases, nothing is unsuitable.Assistant logical to attack, therefore the pain relieving constipation of dispelling, relieving rigidity of muscles and joints, controls contracture, paralysis, the card such as dry, puckery.Nutrient QI being deficient and exterior syndrome being not relieved person, assistant is with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, the Biao Weire that can fall apart greatly, and show not hold back person, and help with Radix Et Rhizoma Rhei and so on, again can consolidating superficial resistance.Precisely because gas Xin Erdong, thus want its quiet person when keeping away it, the sliding benefaction of property, stool is not consolidated person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.

Semen Cassiae: bitter in the mouth, sweet, salty, cold nature, enters liver, kidney, large intestine channel; Loosening bowel to relieve constipation, blood fat reducing improving eyesight, treatment constipation and hyperlipidemia, hypertension.Liver heat removing and eyesight improving, diuretic relieving constipation, has laxative action, reducing blood pressure and blood fat.< < Bencao Jingshu > > records: Semen Cassiae, its salty in the mouth is flat, and it is nontoxic with sweet being slightly cold of hardship that < < does not record > > benefit.The salty aqueous vapor that obtains, rusticly sweet, hardship can expel the heat-evil, the flat gastric qi of closing, cold can expelling the heat-evil by tonifying YIN, the positive medicine of liver meridian man is also.Also enter gallbladder kidney.Liver opening at eye, gb 1 God Light belongs to kidney, therefore main optic atrohic blindness order is excessive, the red tunica albuginea of skin, swelling ophthalmalgia lacrimation.< < does not record the double blue-purple lips for the treatment of of > >.< < herbal classic > > takes beneficial with nothing left person for a long time, tonifying YIN expels the heat-evil, due to the gas of large invigorating the liver and kidney also.< < book on Chinese herbal medicine is stated > > and is recorded: Semen Cassiae, Semen Celosiae, though say it, control the same merit of order, right Semen Celosiae taste, on < < herbal classic > >, cloud hardship is slightly cold, and Semen Cassiae is said salty putting down, at < <, not recording > > and say again bitter sweet being slightly cold, is solid also also different.Condition Jia Mo calls it except liver-heat, and especially and irritability, it cures mainly and is better than Herba Celosiae argenteae.The great thinker of the past calls it and does not damage vigour person with irritability again, and two say that nothing is according to Yu to the greatest extent.Yu Zhiyi teen age boy, have order disease and heals, and because of epistaxis, Liver and kidney asthenic fire is all moving for a long time again, causes conjunctival congestion left eye corner of the eyes hypodynia, in plus-minus pill of Six Ingredients, enters Semen Cassiae, and without Herba Celosiae argenteae, and effect is very fast.

Bulbus Allii: warm in nature, acrid in the mouth is put down; Enter spleen, stomach, lung meridian.Removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, and anthelmintic also has warming the spleen warming the stomach in addition.Control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension, circulation of qi promoting removing food stagnancy, parasite killing removing toxic substances.For flu, bacillary dysentery, amebic dysentery, enteritis, eating accumulation, carbuncle skin infection.Strong disinfecting: in Bulbus Allii, sulfur-containing compound has strong antibacterial and anti-inflammation functions, all has and suppresses and killing action multiple coccus, bacillus, fungus and virus etc., is the strongest a kind of of antibacterial action in the natural plants of finding at present.Anti-curing oncoma and cancer: the elements such as the germanium in Bulbus Allii and selenium can inhibition tumor cell and the growth of cancerous cell, experiment finds, the crowd that cancer incidence is minimum is exactly the highest crowd of selenium content in blood.American National cancerous tissue thinks, in the plant of the anticancer potentiality of tool in the whole world, what rank first is Bulbus Allii.Toxin-expelling intestine-cleaning, prevention of gastrointestinal: Bulbus Allii can effectively suppress and kill the bacterial viruses such as helicobacter pylori that cause gastroenteropathy, removes the intestines and stomach noxious substance, and stimulating gastrointestinal mucosa promotes appetite, accelerates digestion.Reduce blood glucose, prevent diabetes: Bulbus Allii can promote the secretion of insulin, increases the absorption of histiocyte to glucose, improve human glucose tolerance, reduce rapidly blood sugar level in body, and can kill the various pathogenic bacterias because of infection-induced diabetes, thereby effectively prevent and treat diabetes.Control cardiovascular and cerebrovascular disease: Bulbus Allii can prevent the lipidosis in cardiovascular and cerebrovascular vessel, the metabolism of induced tissue fat inside, significantly increase fibrinolytic activity, reduce cholesterol, suppress hematoblastic gathering, reduce plasma concentration, increase arteriolar expansion ratio, impel vasodilation, regulate blood pressure, increase the permeability of blood vessel, thereby suppress formation and the prevention of arterial sclerosis of thrombosis.Eating 2 ~ 3 lobe Bulbus Alliis every day, is the preferably the most easy way of blood pressure lowering, and Bulbus Allii can help the right quantity of a kind of enzyme in holder and avoid occurring hypertension.

Radix Rhodiolae: sweet, puckery, cold.Function cures mainly: clearing away lung-heat to relieve cough, hemostasis, leukorrhagia stopping.For cough due to lung-heat, spitting of blood, leucorrhea; Traumatic injury is controlled in external, burn and scald.

Semen Arecae: bitter in the mouth; Pungent; Warm in nature.Return stomach; Large intestine channel.Effect, anthelmintic; Removing food stagnancy; The therapeutic method to keep the adverse QI flowing downwards; Row water; Preventing the attack (or recurrence) of malaria.Malnutrition due to parasitic infestation; Dyspepsia; Abdominal distention; Heavy after dysentery; Foot gas; Edema; Malaria.Resisting pathogenic microbes effect Semen Arecae infusion (1:1) in vitro to trichophyton, permitted the dermatophytess such as blue achorion and had inhibitory action in various degree.Decoct and some strain of water logging agent infected by influenza A type have certain inhibitory action, and antiviral effect may be relevant with wherein contained tannin.

Flos Lonicerae: sweet in the mouth, cold in nature, enter the heart, lung, stomach, large intestine channel, there is the effect of heat-clearing and toxic substances removing, holding concurrently can eliminating pathogenic heat from blood to cure dysentery, is mainly used in affection due to external wind and heat, epidemic febrile disease pyretic toxicity is contained, or epidemic febrile disease is from the beginning of pathogenic heat still at the part of the body cavity above the diaphragm housing the heart and lungs and when not descending; Pyretic toxicity dysentery, stool hematochezia; Fever disease in summer card; The carbuncle sore that pyretic toxicity gathers is from the beginning of the ， inflammation heat person of swelling and ache, and other skin infections, and this thing has good antipyretic effect, has antiinflammatory action, can promote leukocytic phagocytic function, and multiple pyococcus and pathogenic entero becteria are had to stronger bacteriostasis.

Cortex Fraxini: cure mainly heat clearing and damp drying, liver heat removing and eyesight improving, antiasthmatic-antitussive.For pyretic toxicity dysentery, conjunctival congestion and swelling pain, the raw cataracta of order.

Cortex cinnamomi japonici (Ramulus Cinnamomi): acrid in the mouth, temperature, nontoxic.Enter the heart, liver,spleen,kidney four warps.Function cures mainly: warming the spleen and stomach, dispersing wind and cold, promoting blood circulation.Control the cold fullness in the chest of abdomen, vomit dysphagicly, rheumatic arthralgia, falls that to damage the stasis of blood stagnant, dysentery hemorrhoidal hamorrhage.1. < < Bencao Shiyi > >: " control in abdomen all cold, vim and vigour distending pain." 2. < < Haiyao Bencao, Oversea Materia Medica > >: " mends warm waist foot, and stagnant blood after bankruptcy, controls dysentery hemorrhoidal hamorrhage, and power and Cortex Cinnamomi are same." 3. < < Traditional Chinese Medicine in Sichuan will > >: " liver and kidney tonifying, qualcomm meridian, dispersing wind and cold, except arthralgia chiefly caused by damp pathogen, warm waist knee joint, preventing or arresting vomiting is told.Control bones and muscles pain, diarrhea due to cold pathogen stomachache, cholera vomiting, dysphagic fullness in the chest, bladder colic of cold type, waist knee joint is existing cold, and rheumatic arthralgia and falling damages the diseases such as the stasis of blood is stagnant."

Charred Radix Sanguisorbae: bitter in the mouth, acid, cool, return liver, stomach, large intestine channel.There is cooling blood for hemostasis, the effect of pathogenic fire purging sore.Convergence that Radix Sanguisorbae is sour and astringent, heat clearing away cool in nature, bitter in the mouth sedimentation, the through part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels, controls the card of bleeding due to blood-heat, specially clear part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels heat in blood, for having blood in stool, hematuria, all cards of metrorrhagia.Bitter cool eliminating fire and detoxication, sour and astringent convergence granulation promoting, therefore treatment carbuncle sore tumefacting virus, the particularly good medicine of scald.

Fructus Aurantii Immaturus: bitter, pungent, sour, temperature.Return spleen, stomach warp.Function cures mainly: dispelling the stagnated QI removing food stagnancy, the loose painful abdominal mass of reducing phlegm.In stagnant, stop, feeling of fullness distending pain, heavy after dysentery, constipation, the stagnant vapour lock thoracic obstruction of expectorant, blocked-up chest; Gastroptosis, proctoptosis, uterine prolapse.1. the effect to uterus: with Fructus Aurantii and the Fructus Aurantii Immaturus decocting liquid in three different places of production (Sichuan, Jiangxi, Hunan), mice isolated uterine (pregnant and unpregnancy) is all to inhibitory action; Rabbit is all to excitement at body and isolated uterine (pregnant and unpregnancy).Rabbit uterus fistula is also proved to make uterine contraction strong, and tension force increases, and even occurs tetanic contraction.Fructus Aurantii tincture and Fructus Aurantii fluid extract, also have excitation to rabbit uterus (at body and in vitro); Mouse Uterus (in vitro) is suppressed.Isolated a kind of alkaloid sample material in fructus citri wilsonii and Fructus Ponciri Trifoliatae (Fructus Ponciri Trifoliatae Immaturus), rabbit isolated uterine is also had to certain contraction, especially with the excited uterus muscle of crossing of pituitrin, more showing: remove alkaloidal part, rabbit isolated uterine is but had to relexation, after pituitrin excitement, the effect of relaxing in uterus is more obvious.Isolated Cirantin from Fructus Aurantii Immaturus peel, can suppress the ovary activity of hyaluronidase around, and this may relevant with its contraceptive efficacy (stop and be fertilized).2. the impact on intestinal tube: the Fructus Aurantii in three different places of production and Fructus Aurantii Immaturus, be all inhibition to the intestine in vitro of mice and rabbit; Rabbit is most for suppressing at body intestinal tube, and minority is unchanged.Fructus Aurantii tincture and fluid extract thereof, all suppress mice (in vitro), rabbit (at body and in vitro) intestinal tube.High concentration (1:1000) is all inhibition to isolated rabbit, small intestine of guinea pig, and can suppress the effect of acetylcholine, histamine; Low concentration (1:10000), after of short duration inhibition, can present excitation, and amplitude strengthens, and frequency is accelerated.The intestinal in place of anesthetized dog, also has obvious inhibition with decoct; But the dog to Gastroenteritic, is certain excitation, can make gastrointestinal motility shrink the rhythm and pace of moving things strong.3. the effect to the heart, blood vessel: be excited in a small amount to the in vitro heart of Bufo siccus, suppress in a large number.Fructus Aurantii and Fructus Aurantii Immaturus decocting liquid, Fructus Aurantii tincture and fluid extract are all like this.With Fructus Aurantii decocting liquid or alcohol extract, to dog intravenous injection, can cause remarkable boosting.The real decocting liquid of the Fructus Aurantii in three different places of production and maple, proves there is slight pressor effect with Bufo siccus whole body blood vessel irrigation method; To anesthetized dog, there is significantly and rapidly boosting, " respiration inhibition " that it causes while not having epinephrine to boost, " rear blood pressure lowering " phenomenon, heart rate is also without obviously speeding.Pressure rising mechanism is relevant with following factors: (i) excited α receptor, causes part organ vasoconstriction (aniline azoles quinoline can be " blood pressure lowering " reaction by " boosting " upset); (ii) myocardial contraction is strengthened, and cardiac output increases (guinea pig isolated heart perfusion and heart-lung preparation).After reserpinization, the boosting of Fructus Aurantii Immaturus is more remarkable.Anesthesia Canis familiaris L. Fructus Aurantii Immaturus is had to the effect (bubble flowmeter, coronary flow can increase by 289.4%) of the increase coronary flow of highly significant, brain, renal blood flow also on average increase by 86.4% and 64.5%, have significantly different from pyin on nor-kidney; Femoral artery blood flow has minimizing, and myocardial oxygen consumption slightly increases, but not obvious, capable with the remarkable increase irrelevancy of coronary flow.In the electrocardiogram test of dog and Cavia porcellus, the cardiac arrhythmia that heavy dose of Fructus Aurantii Immaturus causes (ventricular tachycardia or atrioventricular block) is also not serious, and according to above effect feature, someone advises can be used for treating cardiogenic shock.From fructus citri wilsonii, also can make the tensity of vascular smooth muscle have of short duration enhancing with alkaloid separated in Fructus Ponciri Trifoliatae (Fructus Ponciri Trifoliatae Immaturus), especially more obvious after pituitrin effect.4. other effects: citrusreticulata oil platymiscium viscose glue mass-energy reduces cholesterol content in the serum of rat (with containing cholesteric forage feed) and liver.The alcohol immersion of Fructus Aurantii Immaturus has inhibitory action to tubercule bacillus H27Rv in vitro, and its Mlc is 1:1000.Its water decoction on Cavia porcellus calamity trachea without impact.Someone reports, the fruit juice that Citrus chachiensis Hort. Fructus Citri grandis belongs to can promote the fermenting speed of (bread) yeast, boiling rear activity does not decline, therefore the main medical application of non-enzyme component orange juice contains abundant vitamin C at it, and containing vitamin A, the B of a great deal of, skin is containing vitamin C, and be imbued with vitamin A, bitter in the mouth, can stomach invigorating.Children's, as taken into a large amount of peels, can cause poisoning (stomachache, spasm).

Herba Polygoni cymosi: acid, cool.Function cures mainly: heat-clearing and toxic substances removing, inducing diuresis to remove edema.For upper respiratory tract infection, tracheitis, pertussis, acute tonsillitis, enteritis, dysentery, oedema due to nephritis; Herpes zoster is controlled in external, eczema, carbuncle furuncle and phyma poison, venom.

Fructus Coriandri: sad, flat, nontoxic." function cures mainly: rash, stomach invigorating.Treatment of smallpox rash is sent out not smooth thoroughly, and diet is dull, dysentery, hemorrhoid.1. < < a thousand pieces of gold dietetic therapy > >: " rapid digestion of food, the taste of ending a hunger strike." 2. < < Bencao Shiyi > >: " main children's's tinea alba, fry is applied it.Also main insect poison, five pheasant diseases and kreotoxism hematochezia, boil and make son tear open, takes juice." 3. < < detailed outline > >: " sends out varioliform exanthema, kills fish raw meat." 4. < < classification medical herbs > >: " controlled children's's varioliform exanthema and do not gone out, and wards off abnormal climates in four seasons, delivers cold expelling, controls nasal obstruction obstructed." fruit of drying and ripening is weak aromatic, generally can share and make correctives with other medicines.Also gastrointestinal glandular secretion can be promoted, bile secretion can also be promoted.Contained volatile oil has some antifungic action.

Fructus Crataegi: energy prevention and cure of cardiovascular disease, has blood vessel dilating, heart tonifying, increase coronary flow, improves heart vigor, stimulating central nervous system system, reduces blood pressure and cholesterol, vessel softening and diuresis and sedation; Prevent and treat arteriosclerosis, anti-aging, anticancer effect.Crataegolic acid also has cardiotonic, also helpful to senile heart disease.Its energy appetite-stimulating indigestion-relieving, offsets meat stagnation effect better especially, in a lot of digestant medicines, has all adopted Fructus Crataegi; The effect that has activating blood circulation to dissipate blood stasis, contributes to remove local congestion state, and traumatic injury is had to auxiliary curative effect.There is to contraction in uterus, anemia of pregnant woman, has the effect expediting the emergence of just before giving birth time, and can promote uterine involution in puerperal.The generation of free radical is blocked and reduced to the substances such as contained flavonoid and vitamin C, carotene, and the immunity of energy enhancing body, has anti-aging, anticancer effect.In Fructus Crataegi, having relievings asthma reduces phlegm, the composition of anti-bacteria, treatment abdominal pain diarrhea.

Radix Puerariae: nature and flavor are sweet pungent is flat; Be included into spleen, stomach warp.There is yang invigorating expelling pathogenic factors from muscles, rash antidiarrheal, relieving restlessness stops warm effect.Cold, the warm headache stiffness of the nape that cures the wound, dysphoria with smothery sensation is quenched one's thirst, has loose bowels, dysentery, skin eruption is not saturating, hypertension, angina pectoris, deafness.According to < < Compendium of Materia Medica > >, < < Chinese medicine voluminous dictionary > >, the authoritative data books such as < < functional food > >, Radix Puerariae and goods thereof relieve inflammation or internal heat, toxin expelling, blood fat reducing, blood pressure lowering, cholesterol reducing, blood sugar lowering, fat-reducing, relieving constipation, prevention alzheimer disease, prevent arteriosclerosis, prevent the good effect of the cardiovascular and cerebrovascular diseases such as cerebral thrombosis.

Radix Ophiopogonis: sweet in the mouth, micro-hardship, be slightly cold.GUIXIN, lung, stomach warp.Function with cure mainly: YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire.For dryness of the lung dry cough, deficiency of YIN consumptive disease is coughed, sore throat pharyngalgia, and Tianjin wound is thirsty, and interior-heat is quenched one's thirst, vexed insomnia, dryness of the intestine constipation.< < Mingyi Bielu > > records: treat heavy sensation of the body icteric sclera, rami cardiaci inferiores is full, asthenia exopathogenic heat, xerostomia excessive thirst, preventing or arresting vomiting is told, and more flaccidity falls, and reinforcing YIN-essence benefit essence, during rapid digestion of food is adjusted, protect god, determine lung qi, settling five organs, makes us fertile strong.The newly organized > > of < < book on Chinese herbal medicine records: Radix Ophiopogonis, the volt fire in eliminating the pathogens from the lung, the pathogenic heat in clearing stomach, the impairment caused by overstrain of reinforcing the heart gas, the vomiting of hemostasis man, beneficial smart reinforcing YIN-essence, separates tired quenching the thirst, U.S. color, happy skin, reducing the asthenic fever, separates the dryness of the lung, fixed cough, what very can hold is monarch and making also for minister of can borrowing.But the magical effect of common people unknown Radix Ophiopogonis, often use less and can not be successfully for unfortunately also.Do not know that Radix Ophiopogonis is necessary multiplex, strength begins large, and Gai Huofu, in lung, sparkles dry interior liquid, and more than Radix Ophiopogonis, fire can not be made; Heat is vehement in stomach, endures its moon to the greatest extent, and more than Radix Ophiopogonis, fire can not cease.The fire that more has bladder, superinverse is in ambition, and urine drop can not go out, and people thinks that urine fire closes, due to the heat of bladder also, with the medicine of water flowing, do not imitate, with the agent of pathogenic fire reducing, do not imitate, how does this use again? the gas of lid bladder, must the clear respectful order of the part of the body cavity above the diaphragm housing the heart and lungs go, and fire is to decline, and water is lower logical.The clear respectful order of husband's part of the body cavity above the diaphragm housing the heart and lungs is reported in lung also, heat of the lung, and making that lung is respectful is clearly not all right, and bladder fire closes, water also closes.Therefore wish is led to bladder person, gas that must lung heat clearing gold, the medicine of lung heat clearing is a lot of, all damages unhelpfully, if do not have benefit in clear Radix Ophiopogonis eventually, can rush down the fire of bladder, and don't damage the gas of bladder, yet use less it, also can not be successful, lid abnormal smells from the patient Radix Ophiopogonis is flat cold, must be multiplex and begin have Ji also.

Herba Portulacae: acid, cold.Return liver, large intestine channel.Be applicable to dysentery, heat-clearing and toxic substances removing, cooling blood for hemostasis.For toxic-heat and blood stasis, carbuncle furuncle, dermatomycosis, erysipelas, snake bite and insect sting, has blood in stool, hemorrhoidal bleeding, metrostaxis.< < opens the surely new herbal > > that values greatly: " Herba Portulacae, clothes not white all the year round." < < Bencao Jingshu > >: " and not white all the year round, always say its removing heat from blood benefit blood, disease is removed the merit ear that body is light.”

Radix Glycyrrhizae: property is flat, and sweet in the mouth, returns 12 warps.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.For weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, the anxious pain of extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strongly have removing toxic substances, eliminate the phlegm, pain relieving, spasmolytic be so that the pharmacological action such as anticancer, Radix Glycyrrhizae has blood fat reducing, antioxidative effect.

Pot cake: sweet, light, flat.Function cures mainly: thick the intestines and stomach is aid digestion.

Embodiment 1 powder

The embodiment of the present invention provides a kind of colibacillary medicine of lamb that is used for the treatment of, and medicine is comprised of following raw medicinal material:

Rhizoma Coptidis 23 weight portions, the Radix Pulsatillae 15 weight portions, Radix Rhamni Heterophyllae 15 weight portions, Pericarpium Citri Reticulatae 16 weight portions, Radix Paeoniae Rubra 21 weight portions, Radix Angelicae Sinensis 14 weight portions, Semen Cassiae 13 weight portions, Bulbus Allii 12 weight portions, Radix Rhodiolae 15 weight portions, Semen Arecae 13 weight portions, Flos Lonicerae 12 weight portions, Cortex Fraxini 12 weight portions, Cortex cinnamomi japonici (Ramulus Cinnamomi) 17 weight portions, charred Radix Sanguisorbae 15 weight portions, Fructus Aurantii Immaturus 15 weight portions, Herba Polygoni cymosi 15 weight portions, Fructus Coriandri 16 weight portions, Fructus Crataegi 16 weight portions, Radix Puerariae 15 weight portions, Radix Ophiopogonis 14 weight portion, Herba Portulacae 14 weight portions, Radix Glycyrrhizae 18 weight portions and pot cake 13 weight portions.

The embodiment of the present invention also provides a kind of preparation method that is used for the treatment of the medicine of lamb colibacillosis, and the dosage form of medicine is powder, and preparation method comprises the following steps:

All raw medicinal materials are mixed in proportion, are ground into 150 object powder, add the water with respect to 4 times of mixture qualities, at the temperature of 95 ℃, decoct 40 minutes, decoction liquor is filtered, and obtains the first filtrate and filtering residue;

Second step, the filtering residue that the first step is obtained adds the water with respect to 2 times of first step mixture qualities, at the temperature of 95 ℃, decocts 40 minutes, and decoction liquor is filtered, and obtains the second filtrate;

The 3rd step, merges the first filtrate and the second filtrate, and concentrating under reduced pressure, except desolventizing, obtains and gets dry extract, and dry cream is put into spray dryer dry, guarantees that inlet temperature is at 180 ℃, and 70 ℃ of leaving air temps, are ground into 300 orders subsequently, obtain drug powder.

Toxicity test

Select SD rat, give the aqueous solution of the powder of the present invention of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, after last administration, 24 hours each groups are lived and are killed 1/2 animal (male and female half and half), and all the other 1/2 animals continue observation and live and kill after 2 weeks.Duration of test is observed outward appearance, general behavior, food ration, the body weight change of animal, and after administration, the index checkings such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology are carried out in 90 days and drug withdrawal for 2 weeks.Result of the test shows: the medicine of the embodiment of the present invention 1 is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight gain are all without ANOMALOUS VARIATIONS; Three dosage groups and matched group hematological examination, blood biochemical are learned, urine biochemical analysis is all in normal range, between group without significant difference; Each is organized main organs histopathological examination and shows no obvious abnormalities.These parameters drug withdrawal also has no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, according to the medicine of the result of the test embodiment of the present invention 1, in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days, rat is had no significant effect, without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no retardance toxic reaction, points out the dosage safety of clinical drug application of the present invention higher.

Embodiment 2 injections

The embodiment of the present invention also provides a kind of colibacillary medicine of lamb that is used for the treatment of, medicine is comprised of the raw material of following parts by weight: Rhizoma Coptidis 15 weight portions, the Radix Pulsatillae 13 weight portions, Radix Rhamni Heterophyllae 18 weight portions, Pericarpium Citri Reticulatae 18 weight portions, Radix Paeoniae Rubra 18 weight portions, Radix Angelicae Sinensis 13 weight portions, Semen Cassiae 12 weight portions, Bulbus Allii 10 weight portions, Radix Rhodiolae 17 weight portions, Semen Arecae 12 weight portions, Flos Lonicerae 13 weight portions, Cortex Fraxini 14 weight portions, Cortex cinnamomi japonici (Ramulus Cinnamomi) 19 weight portions, charred Radix Sanguisorbae 17 weight portions, Fructus Aurantii Immaturus 13 weight portions, Herba Polygoni cymosi 18 weight portions, Fructus Coriandri 17 weight portions, Fructus Crataegi 17 weight portions, Radix Puerariae 17 weight portions, Radix Ophiopogonis 12 weight portion, Herba Portulacae 12 weight portions, Radix Glycyrrhizae 15 weight portions and pot cake 17 weight portions.

The embodiment of the present invention also provides a kind of preparation method that is used for the treatment of the medicine of lamb colibacillosis, and the dosage form of medicine is injection, and described preparation method comprises the following steps:

The first step, is mixed in proportion pulverizing by all raw medicinal materials, decocts with water 3 times, each decocting time is 2.5 hours, the water quality adding is 3 times of first step mixture quality, merges decoction liquor, filters, filtrate is concentrated into syrupy shape, adding determining alcohol is more than 95% ethanol, and the ethanol content adding is 2 times of mixture quality of the first step, after standing 18 hours, centrifugal filtration, filtrate is adjusted to filtrate pH7 with 45% NaOH;

Second step, the filtrate that the first step is obtained, adds 1% active carbon and boils 20 minutes, filters, and decarburization, injects water, and the crude drug concentration that makes injection is 3g/ml, and fill sterilizing 2 hours in 500ml saline bottle, after freezing a couple of days, filters.

Clinical trial

1.1 for examination material

1.1.1 for examination animal

In sheep raising field, Shandong Province, 340 of random choose healthy lambs in 3 week age.

1.1.2 strains tested

Sheep raising field, Xinjiang finds that different symptoms appears in more than 30 3 days old lambs, and has the phenomena of mortality, and clinical main manifestations is: fervescence to 42 ℃, and lethargy, dyspnea, extremity are stiff, move inharmonious, and inappetence, shakes, foam at the mouth.Through cuing open, examine the lamb that dies of illness, find that stomach, small intestinal, large intestinal contents are lark, mucous hyperemia, mesentery enlargement is congested, the swollen gas of caecum, swollen lymph node, renal cortex is congested.Through being diagnosed as lamb colibacillosis.Micro biological Tests is got inspection pathological material of disease tissue and is distinguished smear, by two healthy lambs of separated challenge with E.coli, with 48 hours in there is escherichia coli disease symptoms, determine that dead lamb is that colibacillosis is lethal.Using separated escherichia coli as strains tested.

1.1.3 for reagent product

The powder of embodiment 1; The injection of embodiment 2; Conventional medicament: oxytetracycline is by per kg body weight per day 20-50 milligram, minute 2-3 time oral.

1.2 test method

1.2.1 inoculation test

By the escherichia coli of cultivating respectively 340 of intravenous injections for examination lamb, infect and have afterwards one day after 317 lambs to occur the symptom of colibacillosis, select wherein 300 ill lambs as experiment sheep.

1.2.2 experiment grouping

300 ill lambs are divided into three groups at random, 100 every group, i.e. embodiment group 1, embodiment group 2 and matched group.

1.2.3 test method

The lamb that occurs colibacillosis after infection adopts respectively different Drug therapys, and wherein, embodiment group 1 is used the powder of the embodiment of the present invention 1 to treat, powder is dissolved in water and is gavaged according to the percentage by weight of 1:4, every day twice, gavages 5g powder every day, uses continuously 3-7 days; Embodiment group 2 is used the injection of the embodiment of the present invention 2 to treat, intravenous injection, and each 2-5ml, once a day, is used 3-7 days continuously; Matched group is used oxytetracycline, by per kg body weight per day 20-50 milligram, divide 2-3 time oral, use continuously 3-7 days.

1.2.4 observed and recorded

After infecting, clinical symptoms and the pathological change of lamb respectively organized in observation, the therapeutic effect after the rear death toll of record infection, treatment finish.

2 results

2.1 criterion of therapeutical effect

Cure: treat after 3 days, ill lamb body temperature, spirit and appetite are all recovered normally, without symptom of diarrhea, to be judged to healing;

Effective: treat after 3-5 days, ill lamb body temperature, spirit and appetite are recovered normally, not suffer from diarrhoea substantially, though or have diarrhoea feces to be substantially shaped, be judged to effectively;

Invalid: ill lamb symptom does not disappear or sb.'s illness took a turn for the worse even death be judged to invalid.

Wherein, effective percentage is for curing and effective percentage ratio sum.

2.2 observed results are as shown in table 1.

Data from table 1: compare with matched group, the medicine of the embodiment of the present invention 1 and embodiment 2 has evident in efficacy to the treatment also of lamb escherichia coli, and side effect is low, the advantage that treated effect is high; In long-term observation subsequently, find, the lamb that embodiment group 1 and embodiment group 2 cured again sickness rate is starkly lower than the lamb of matched group.

2.3 infect rear symptom and cut open inspection result

After infection, the symptom of ill lamb is: sick just body temperature is slightly high, occurs the rear temperature decline of diarrhoea, and feces is semi-liquid-like, and band bubble, is mixed with blood sometimes, lamb performance stomachache, and weakness, serious dehydration, can not stand up; Some lamb is dead in 24-36 hour after infection.

Concrete case

Case 1

11 lambs of ewe symbiosis lambing sheep of Lee's autotrophy, found after the 4 week age of being born, and following symptom in various degree appears in lamb: fervescence to 41 ℃, spirit is depressed, there is symptom of diarrhea in three lambs, moves inharmonious, is diagnosed as lamb colibacillosis, use the powder treatment of the embodiment of the present invention 1, powder is dissolved in water and is gavaged according to the percentage by weight of 1:4, every day twice, gavage 5g powder every day, use 3 days continuously, it is normal that lamb all recovers.

Case 2

Till 3 days Augusts in 2012 of certain sheep raising field,, there is temperature decline after diarrhoea in 72 appearance of lamb of total 2-8 age in days following symptom in various degree: sick just body temperature is slightly high, and feces is semi-liquid-like, band bubble, is mixed with blood sometimes, lamb performance stomachache, weakness, serious dehydration, can not stand up; Two lamb death, find through dissecting, dead lamb breast, abdominal cavity and a large amount of hydrops of pericardium, inside have cellulose; Arthroncus, includes turbid liquid or purulence flocculus; Meninges is congested, has a lot of little petechias.Be diagnosed as lamb colibacillosis, use the injection treatment of the embodiment of the present invention 2, intravenous injection, each 2ml, once a day, is used 3 days continuously, and 57 lambs recover normal, and 13 lamb continual cures 2 two days, also all recover normal.

The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.

Claims (6)

1. the medicine that is used for the treatment of lamb colibacillosis, it is characterized in that, described medicine comprises following raw medicinal material: Rhizoma Coptidis, the Radix Pulsatillae, Radix Rhamni Heterophyllae, Pericarpium Citri Reticulatae, Radix Paeoniae Rubra, Radix Angelicae Sinensis, Semen Cassiae, Bulbus Allii, Radix Rhodiolae, Semen Arecae, Flos Lonicerae, Cortex Fraxini, Cortex cinnamomi japonici (Ramulus Cinnamomi), charred Radix Sanguisorbae, Fructus Aurantii Immaturus, Herba Polygoni cymosi, Fructus Coriandri, Fructus Crataegi, Radix Puerariae, Radix Ophiopogonis, Herba Portulacae, Radix Glycyrrhizae and pot cake.

2. the medicine that is used for the treatment of lamb colibacillosis according to claim 1, it is characterized in that, described medicine is comprised of the raw medicinal material of following parts by weight: Rhizoma Coptidis 10-30 weight portion, Radix Pulsatillae 10-20 weight portion, Radix Rhamni Heterophyllae 10-20 weight portion, Pericarpium Citri Reticulatae 10-20 weight portion, Radix Paeoniae Rubra 15-25 weight portion, Radix Angelicae Sinensis 10-20 weight portion, Semen Cassiae 10-15 weight portion, Bulbus Allii 10-15 weight portion, Radix Rhodiolae 10-20 weight portion, Semen Arecae 10-15 weight portion, Flos Lonicerae 10-15 weight portion, Cortex Fraxini 10-15 weight portion, Cortex cinnamomi japonici (Ramulus Cinnamomi) 15-20 weight portion, charred Radix Sanguisorbae 10-20 weight portion, Fructus Aurantii Immaturus 10-20 weight portion, Herba Polygoni cymosi 10-20 weight portion, Fructus Coriandri 10-20 weight portion, Fructus Crataegi 10-20 weight portion, Radix Puerariae 10-20 weight portion, Radix Ophiopogonis 10-20 weight portion, Herba Portulacae 10-20 weight portion, Radix Glycyrrhizae 10-20 weight portion and pot cake 10-20 weight portion.

3. the medicine that is used for the treatment of lamb colibacillosis according to claim 1 and 2, it is characterized in that, described medicine is comprised of the raw medicinal material of following parts by weight: Rhizoma Coptidis 15-25 weight portion, Radix Pulsatillae 13-17 weight portion, Radix Rhamni Heterophyllae 12-18 weight portion, Pericarpium Citri Reticulatae 13-18 weight portion, Radix Paeoniae Rubra 18-23 weight portion, Radix Angelicae Sinensis 13-16 weight portion, Semen Cassiae 12-15 weight portion, Bulbus Allii 10-13 weight portion, Radix Rhodiolae 13-17 weight portion, Semen Arecae 12-15 weight portion, Flos Lonicerae 10-13 weight portion, Cortex Fraxini 11-14 weight portion, Cortex cinnamomi japonici (Ramulus Cinnamomi) 16-19 weight portion, charred Radix Sanguisorbae 13-17 weight portion, Fructus Aurantii Immaturus 13-17 weight portion, Herba Polygoni cymosi 12-18 weight portion, Fructus Coriandri 13-17 weight portion, Fructus Crataegi 14-17 weight portion, Radix Puerariae 13-17 weight portion, Radix Ophiopogonis 12-17 weight portion, Herba Portulacae 12-16 weight portion, Radix Glycyrrhizae 15-20 weight portion and pot cake 10-17 weight portion.

4. according to the medicine that is used for the treatment of lamb colibacillosis described in claim 1-3 any one, it is characterized in that, described medicine is comprised of the raw medicinal material of following parts by weight: Rhizoma Coptidis 23 weight portions, the Radix Pulsatillae 15 weight portions, Radix Rhamni Heterophyllae 15 weight portions, Pericarpium Citri Reticulatae 16 weight portions, Radix Paeoniae Rubra 21 weight portions, Radix Angelicae Sinensis 14 weight portions, Semen Cassiae 13 weight portions, Bulbus Allii 12 weight portions, Radix Rhodiolae 15 weight portions, Semen Arecae 13 weight portions, Flos Lonicerae 12 weight portions, Cortex Fraxini 12 weight portions, Cortex cinnamomi japonici (Ramulus Cinnamomi) 17 weight portions, charred Radix Sanguisorbae 15 weight portions, Fructus Aurantii Immaturus 15 weight portions, Herba Polygoni cymosi 15 weight portions, Fructus Coriandri 16 weight portions, Fructus Crataegi 16 weight portions, Radix Puerariae 15 weight portions, Radix Ophiopogonis 14 weight portion, Herba Portulacae 14 weight portions, Radix Glycyrrhizae 18 weight portions and pot cake 13 weight portions.

5. a preparation method for the medicine that is used for the treatment of lamb colibacillosis as described in claim 1-4 any one, is characterized in that, the dosage form of described medicine is powder, and described preparation method comprises the following steps:

All raw medicinal materials are mixed in proportion, are ground into 100 order～200 object powder, add the water with respect to 3～5 times of mixture qualities, at the temperature of 90 ℃～100 ℃, decoct 30 minutes～1 hour, decoction liquor is filtered, and obtains the first filtrate and filtering residue;

Second step, the filtering residue that the first step is obtained adds the water with respect to 1～3 times of first step mixture quality, at the temperature of 90 ℃～100 ℃, decocts 30 minutes～1 hour, and decoction liquor is filtered, and obtains the second filtrate;

The 3rd step, merges the first filtrate and the second filtrate, and concentrating under reduced pressure is except desolventizing, obtain and get dry extract, dry cream is put into spray dryer dry, guarantee that inlet temperature is at 170 ℃～190 ℃, 60 ℃～80 ℃ of leaving air temps, are ground into 200 order～400 orders subsequently, obtain drug powder.

6. a preparation method for the medicine that is used for the treatment of lamb colibacillosis as described in claim 1-4 any one, is characterized in that, the dosage form of described medicine is injection, and described preparation method comprises the following steps:

The first step, is mixed in proportion pulverizing by all raw medicinal materials, decocts with water 2～4 times, each decocting time is 2 hours～3 hours, the water quality adding is 2～4 times of first step mixture quality, merges decoction liquor, filters, filtrate is concentrated into syrupy shape, adding determining alcohol is more than 95% ethanol, and the ethanol content adding is 1～2 times of mixture quality of the first step, after standing 12 hours～24 hours, centrifugal filtration, filtrate is adjusted to filtrate pH7～8 with 40%～50% NaOH;

Second step, the filtrate that the first step is obtained, adds 0.5%～1% active carbon and boils 18～22 minutes, filters, decarburization, injects water, and the crude drug concentration that makes injection is 2g/ml～4g/ml, fill sterilizing 1.5 hours～2 hours in 500ml saline bottle, after freezing a couple of days, filters.