CN103690707A - Natural pharmaceutical composition - Google Patents
Natural pharmaceutical composition Download PDFInfo
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- CN103690707A CN103690707A CN201310663470.1A CN201310663470A CN103690707A CN 103690707 A CN103690707 A CN 103690707A CN 201310663470 A CN201310663470 A CN 201310663470A CN 103690707 A CN103690707 A CN 103690707A
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- ethyl acetate
- cortex phellodendri
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Abstract
The invention relates to a natural pharmaceutical composition. The natural pharmaceutical composition is composed of 2-7 parts of rhizoma alismatis/phellodendron bark water decoction concentrated extract, 1-4 parts of rhizoma alismatis terpineol B acetate and 4-9 parts of phellodendron bark ethyl acetate extract. The composition has favorable curative effect on cancer chemotherapy related diarrhoea.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition being comprised of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract, the preparation method of this natural drug composition and this natural drug composition reduce the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic is cell toxicant antitumor drug, alone or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and after chemotherapy of tumors, diarrhoea is again Chemotherapy-induced Diarrhea.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, also to CC, bring certain difficulty, therefore effective treatment of Chemotherapy-induced Diarrhea has been become the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no at present the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea effect.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for of being easy to promote alleviates cancer chemotherapy induced diarrhea becomes clinical problem demanding prompt solution.
Rhizoma Alismatis is the dry rhizome of ranunculaceae plant Rhizoma Alismatis Coptis chinensis Franch., triangle leaf Rhizoma Alismatis Coptis deltoidea C. Y. Cheng et Hsiao or Coptis Teeta Wall Coptis teeta Wall., and hardship is cold.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel, the disease such as, dysentery damp and hot for the intestines and stomach.Cortex Phellodendri, is the dry bark of rutaceae wampee or Cortex Phellodendri, energy heat and toxic materials clearing away medicine; Heat clearing and dampness removing medicine.Cure mainly: hygropyretic dysentery, have loose bowels.The inventor, through research and the experiment of each a large amount of formula proportion, has finally obtained optimal drug ratio compositions of the present invention, is the compositions of novelty, and has proved that it has fabulous curative effect to cancer chemotherapy induced diarrhea, has completed the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, consists of in the ratio of 2-7:1-4:4-9 by weight Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract.
Above-mentioned natural drug composition, wherein the usage ratio of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract is 7:4:9.
Wherein, Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas, can be by well known to a person skilled in the art the conventional method acquisition of purifying, the natural drug of the present invention that adopts these compositions that conventional method obtains or the one-tenth that adopts business to buy to be grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract is:
According to proportion compatibility, be 1-5: 1 gets Rhizoma Alismatis, Cortex Phellodendri, distilled water immersion 1h, heating decocts 1h, and silk filters, medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, drying under reduced pressure, obtains.
In above-mentioned natural drug composition, the preparation method of alisol B acetas is:
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain.
In above-mentioned natural drug composition, Cortex Phellodendri ethyl acetate extract preparation method is:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain Cortex Phellodendri ethyl acetate extract.
The present invention also provides the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract
According to proportion compatibility, be 1-5: 1 gets Rhizoma Alismatis, Cortex Phellodendri, distilled water immersion 1h, heating decocts 1h, and silk filters, medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of alisol B acetas
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain.
(3) Cortex Phellodendri ethyl acetate extract preparation
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain Cortex Phellodendri ethyl acetate extract.
(4) choose in proportion Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract, evenly mix, obtain.
The preparation method of above-mentioned natural drug composition, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
Natural drug composition of the present invention is as follows at the beneficial effect of anti-Chemotherapy-induced Diarrhea:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, the inventor combines Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract originally, and determine the consumption proportion between these components by great many of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention has produced unexpected synergism when 2-7:1-4:4-9, potentiation is obvious, especially especially obvious when the usage ratio of each component is 7:4:9, this is that the inventor pays a large amount of creative works and obtains, and those of ordinary skills only cannot simply obtain this ratio range and optimum amount ratio by disclosed content.
3, this natural drug composition Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and each component of Cortex Phellodendri ethyl acetate extract all can obtain by conventional means, and such compositions itself has the convenience of acquisition, lower-cost effect.This is also the product that has splendid curative effect and higher industrial practicality concurrently that inventor obtains by a large amount of creative work sides.
The specific embodiment
Below by embodiment and test example, further set forth the preparation of natural drug composition of the present invention, with and at diarrhea beneficial effect.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract
According to proportion compatibility, be 1-5: 1 gets Rhizoma Alismatis, Cortex Phellodendri, distilled water immersion 1h, heating decocts 1h, and silk filters, medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, drying under reduced pressure, obtains.
(2) preparation of alisol B acetas
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain.
(3) Cortex Phellodendri ethyl acetate extract preparation
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain Cortex Phellodendri ethyl acetate extract.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take respectively Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract 35mg, alisol B acetas 20mg and Cortex Phellodendri ethyl acetate extract 45mg prepared by above-mentioned steps, take starch 395mg, magnesium stearate 5mg simultaneously, evenly mix filled capsules.
(2) preparation of natural drug composition tablet of the present invention
Take respectively Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract 35mg, alisol B acetas 20mg and Cortex Phellodendri ethyl acetate extract 45mg prepared by above-mentioned steps, take starch 295mg, lactose 100mg simultaneously, evenly mix, with wet granulation, dry, after granulate, mix tabletting with 5mg magnesium stearate.
Drug effect and pharmacology's data:
In following test example, compositions is: the usage ratio of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract is 7:4:9.Inventor's compositions that the multiple proportioning combination between 2-7:1-4:4-9 time obtains to above-mentioned each composition usage ratio carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, can play good effect to cancer chemotherapy induced diarrhea.Below the pharmacodynamics data preferred embodiment obtaining:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Rhizoma Alismatis, Cortex Phellodendri, is purchased from medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, A group is model group, and B group is Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is alisol B acetas 10g.kg
-1(being equivalent to containing crude drug amount), D group Cortex Phellodendri ethyl acetate extract 10g.kg
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.B, C, D, E group by corresponding extract gavage 8 days, is placed in metabolic cage by mice continuously, spreads white filter paper to observe stool situation, and observe mice crissum situation and Mus tail fecal pollution situation at the bottom of cage, and in conjunction with cotton swab stimulation anus defecation, every day, observed and recorded was 2 times.The scoring of diarrhoea degree standard is as follows: 0 minute: defecate normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: moderate diarrhoea, stool wets and not molding.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with severe crissum.
3, experimental result
With model group comparison, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mice Chemotherapy-induced Diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount.
Claims (5)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition consists of in the ratio of 2-7:1-4:4-9 by weight Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract,
Wherein the preparation method of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract is:
According to proportion compatibility, be 1-5: 1 gets Rhizoma Alismatis, Cortex Phellodendri, distilled water immersion 1h, heating decocts 1h, and silk filters, medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, drying under reduced pressure, obtains;
Wherein the preparation method of alisol B acetas is:
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain;
Wherein the preparation method of Cortex Phellodendri ethyl acetate extract is:
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas and Cortex Phellodendri ethyl acetate extract is 7:4:9.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that the method comprises:
(1) preparation of Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract
According to proportion compatibility, be 1-5: 1 gets Rhizoma Alismatis, Cortex Phellodendri, distilled water immersion 1h, heating decocts 1h, and silk filters, medicinal residues are adding distil water again, with method, soaks, decocts, filters, and twice filtrate is mixed, and heating is concentrated into small size, ultrafiltration membrance filter, drying under reduced pressure, obtains;
(2) preparation of alisol B acetas
Rhizoma Alismatis coarse powder 80% ethanol percolation, reclaim ethanol to 10%, silicagel column on the oil-like extracts obtaining, take volumetric ratio after 10:1 petroleum ether-ethyl acetate eluting, then take ratio of solvent as 10:3 petroleum ether-ethyl acetate eluting, thin layer chromatography detects, collect fraction, obtain crystallization, then use petroleum ether-ethyl acetate recrystallization, obtain;
(3) preparation of Cortex Phellodendri ethyl acetate extract
Cortex Phellodendri is pulverized, and 95% alcohol-pickled 3 times, merge ethanol extract, the sepia extractum of distilling under reduced pressure, after aqueous dispersion, water, ether and ethyl acetate are extracted successively, and the distilling under reduced pressure of ethyl acetate part, is drying to obtain;
(4) choose in proportion Rhizoma Alismatis Cortex Phellodendri water boiling concentration extract, alisol B acetas evenly mixes with Cortex Phellodendri ethyl acetate extract, obtain.
4. the preparation method of natural drug composition as claimed in claim 3, also comprises: the product that step (4) is made further makes by conventional pharmacy means capsule or the tablet that contains natural drug composition 100mg or 200mg specification.
5. the natural drug composition as described in claim 1-2 any one reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
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| CN103223082A (en) * | 2013-03-27 | 2013-07-31 | 张宗升 | Extract product of traditional Chinese medicine |
| CN103263541A (en) * | 2013-03-29 | 2013-08-28 | 阚兆云 | Extract from traditional Chinese medicine and its preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103223082A (en) * | 2013-03-27 | 2013-07-31 | 张宗升 | Extract product of traditional Chinese medicine |
| CN103263541A (en) * | 2013-03-29 | 2013-08-28 | 阚兆云 | Extract from traditional Chinese medicine and its preparation method |
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Application publication date: 20140402 |