CN103689012A - Drug for preventing and treating stem rot and soft rot of Anoectochilus roxburghii, and formula, preparation method and application thereof - Google Patents
Drug for preventing and treating stem rot and soft rot of Anoectochilus roxburghii, and formula, preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the fields of plant disease control and biopesticides and relates to a drug for preventing and treating stem rot and soft rot of Anoectochilus roxburghii, and a formula, a preparation method and application thereof. According to the invention, the technical problems of modest prevention and treatment effects, environmental pollution and the like of conventional pesticides applied to stem rot and soft rot of Anoectochilus roxburghii are overcome. The formula comprises garlic, onion, Chinese gall and common threewingnut root. A cold extraction method is employed to obtain a first treatment fluid including garlic and onion; a hot decoction method is employed to obtain a second treatment fluid including Chinese gall and common threewingnut root; and the first treatment fluid and the second treatment fluid are used for preventing and treating stem rot and soft rot of Anoectochilus roxburghii. The invention has the following advantages: design is novel, the formula is reasonable, substantial effects are obtained, cost is low, the drug is environment friendly, and the preparation method is simple to operate and easy to master, has good repeatability and is suitable for large scale popularization and application.
Description
Technical field
The invention belongs to control of plant disease and biological pesticide technical field, relate to roxburgh anoectochilus terminal bud stem rot and Disease-Control for Soft-Rotting, especially relate to a kind of medicine of preventing and treating roxburgh anoectochilus terminal bud stem rot and soft rot and formula thereof, preparation method and application.
Background technology
Roxburgh anoectochilus terminal bud (Anoectochilus roxburghii) has another name called rough melic herb, for the orchid family is opened lip Cymbidium herbaceos perennial, it is China's tradition rare Chinese medicine, there is the effects such as clearing heat and cooling blood, removing dampness and detoxicating, be used for the treatment of the diseases such as diabetes, ephritis, cystitis, in the laudatory title of among the people enjoying " king of medicine ".Zhe Nan, Min Tai and Guangdong and Guangxi Provinces one band long-term to boil water with roxburgh anoectochilus terminal bud, make tea, Baoshang, for improving the health, nourishing, take good care of.Modern study shows to contain the Multiple components such as polysaccharide, flavonoids, cardiac glycosides in roxburgh anoectochilus terminal bud, can strengthen body immunity, improves Abwehrkraft des Koepers, prevents the invasion and attack of disease, strengthens and improve the effects such as physique.
Roxburgh anoectochilus terminal bud relies on for a long time excavates collection wild resource, and wild roxburgh anoectochilus terminal bud reproduction rate is extremely low, causes resource reserves to decline rapidly, and parts of traditional producing region can be adopted without medicine.Many scholars study roxburgh anoectochilus terminal bud artificial cultivation in recent years, in key technologies such as seedling fostering, group training transplantation of seedlings and facility cultivations, make a breakthrough.Roxburgh anoectochilus terminal bud is between planting season, and stem rot and soft rot harm are serious, and roxburgh anoectochilus terminal bud stem rot and soft rot are infected and caused by Fusarium oxysporum and the mutation of soft rot Erwinia black stem respectively.Stem rot is infected and causes from basal part of stem by Fusarium oxysporum, and during morbidity, the water stain shape scab of yellowish-brown appears in plants stems base portion, is developed to very soon around stem one week, disease portion tissue rots to dry up to overflow to contract and is wire, rapidly,, there is the phenomenon of dampinging off in the seedling death that lodges rapidly for patient's condition development.Soft rot is caused by the mutation of soft rot Erwinia black stem, mainly by insect, rainwater, farm implements etc., cause the water hole of wound and plant leaf and pore to infect, the illness initial stage shows as pitchy spot, just as water stain shape, then expand, jeopardize whole blade, make the rapid soft corruption of blade, there is obvious juice stream, finally cause plant dead.Roxburgh anoectochilus terminal bud stem rot and soft rot generally can cause production declining 40%～50%, when serious, can reach 80%～90%, cause great economic loss.
Germ killing drugs are all generally mainly from 4 aspects, to illustrate that according to the biological nature of bacterium it kills or suppress the growth of Fusarium oxysporum: the firstth, suppress the generation of spore, the secondth, suppress the sprouting of spore, the 3rd is the growth that suppresses mycelia, and the 4th is the virulence factor (producing malicious ability and zymin) that suppresses Fusarium oxysporum.For roxburgh anoectochilus terminal bud stem rot and soft rot, still take chemical control as main in production at present, the conventional main bactericide such as carbendazim, tpn, mancozeb, streptomycin, thiophanate methyl that adopt are prevented and treated.Because use these medicaments for years, control efficiency reduced year by year, and there is the problems such as residue of pesticide.Therefore find a kind of efficient, safety, the easy method of preparation, control roxburgh anoectochilus terminal bud stem rot and soft rot, for improving roxburgh anoectochilus terminal bud yield and quality, increase economic efficiency significant.
Summary of the invention
The object of the invention is for the problems referred to above, a kind of formula that can prevent and treat the control roxburgh anoectochilus terminal bud stem rot of roxburgh anoectochilus terminal bud stem rot and soft rot and environmentally safe and the medicine of soft rot is provided.
Another object of the present invention is to provide that a kind of technological operation is simple, and production cost is low, is suitable for the preparation method of the control roxburgh anoectochilus terminal bud stem rot of industrialized production and the medicine of soft rot.
The present invention also have an object be to provide a kind of quality controllable, dosage is little, easy to use, can prevent and treat the medicine of roxburgh anoectochilus terminal bud stem rot and soft rot.
The present invention has an object to be to provide a kind of method of preventing and treating of roxburgh anoectochilus terminal bud stem rot simple to operation and soft rot again.
For achieving the above object, the present invention has adopted following technical proposal: the preparation formula of control roxburgh anoectochilus terminal bud stem rot and soft rot medicine, it is characterized in that, and this preparation formula comprises the component of following mass fraction: garlic 80-120 part; Onion 80-120 part; Gall nut 48-72 part; Thunder godvine 48-72 part.Garlic has strong disinfecting effect, and wherein sulfur-containing compound has strong antibacterial and anti-inflammation functions, and multiple coccus, bacillus, fungi and virus etc. all had and suppressed and killing action, is the strongest a kind of of antibacterial action in natural plants; In onion, contain allicin, also there is very strong bactericidal action; The gallic acid that gall nut contains has good inhibition to stem rot pathogenic bacteria; Thunder godvine phytopathogen mycelial growth is had to inhibitory action, can play good bactericidal action.The mutual compatibility of each component is reasonable, and interaction can effectively be prevented and treated roxburgh anoectochilus terminal bud stem rot and soft rot.In the present invention, also can add kuh-seng, the matrine in kuh-seng is inhibited to soft rot pathogenic bacteria, and the fungicidal spectrum of matrine is wider simultaneously.
In above-mentioned control roxburgh anoectochilus terminal bud stem rot and the preparation of soft rot medicine formula, the optimization component of this preparation formula is: 100 parts, garlic; 100 parts of onions; 60 parts of gall nut; 60 parts of thunder godvines.Under this content proportioning, exceed stem rot and the soft rot object of wasting medicinal material and can reach effective control roxburgh anoectochilus terminal bud.
The control roxburgh anoectochilus terminal bud stem rot and the soft rot process for preparing medicine that adopt the preparation formula of above-mentioned control roxburgh anoectochilus terminal bud stem rot and soft rot medicine, is characterized in that, this preparation method comprises the steps:
The preparation of A, the first treatment fluid: get the water that garlic that parts by weight are 80-120 part, onion that parts by weight are 80-120 part and parts by weight are 320-480, then after garlic, onion and water being mixed, carry out pulverization process and obtain homogenate, homogenate is obtained after centrifugal treating to supernatant, obtain the first treatment fluid; Garlic and onion mix extracts the bactericidal action that the first treatment fluid of making has wide spectrum, and stem rot and the soft rot of control roxburgh anoectochilus terminal bud are had to basic defense reaction, and the solvent using is water, cheap, simple and easy to get.
The preparation of B, the second treatment fluid: getting gall nut and the parts by weight that parts by weight are 48-72 part is the thunder godvine of 48-72 part, is immersed in gall nut and thunder godvine in the water that parts by weight are 400-600, after immersion a period of time, decocts, and obtains first liquid; Then at the dregs of a decoction, adding parts by weight is the water of 200-400, again decocts, and obtains secondary liquid; First liquid and secondary liquid are mixed, be settled to the admixing medical solutions that parts by weight are 800-1200, obtain the second treatment fluid.Gall nut, thunder godvine are mixed to extract and make the second treatment fluid, contain gallic acid, tannic acid, triperygium wilfordii extractive with and reactant separately, many kinds of substance synergy, improved the control efficiency of stem rot and soft rot, the solvent using is water, cheap, simple and easy to get.The decocting method using easily operates, and it is in order to extract completely, not waste medicinal material that secondary decocts.
In above-mentioned control roxburgh anoectochilus terminal bud stem rot and the preparation method of soft rot medicine, this preparation method comprises following Optimization Steps:
The preparation of A, the first treatment fluid: get parts by weight and be the water that the garlic of 100 parts, onion that parts by weight are 100 parts and parts by weight are 400, then after garlic, onion and water being mixed, carry out pulverization process and obtain homogenate, homogenate is obtained after centrifugal treating to supernatant, obtain the first treatment fluid;
The preparation of B, the second treatment fluid: get parts by weight and be the thunder godvine that the gall nut of 60 parts and parts by weight are 60 parts, it is in 500 water that gall nut and thunder godvine are immersed in to parts by weight, after soaking a period of time, decoct for the first time, obtain after filtering first liquid; Then at the dregs of a decoction, adding parts by weight is 300 water, again decocts, and obtains after filtering secondary liquid; First liquid and secondary liquid are mixed, be settled to parts by weight and be 1000 admixing medical solutions, obtain the second treatment fluid.The usage amount of raw material has an optimum value, in the scope of application, be greater than the waste that optimum value can cause raw material, be less than optimum value and do not reach best control efficiency, in this Optimization Steps, in the first treatment fluid and the second treatment fluid, best proportioning can either reach optimum efficiency and not waste medicinal material, has saved resource.
In above-mentioned control roxburgh anoectochilus terminal bud stem rot and the preparation method of soft rot medicine, in above-mentioned steps A, described pulverization process is carried out in high speed disintegrator, and the rotating speed of high speed disintegrator is 4500-5500r/min; Described centrifugal treating is carried out in centrifugation apparatus, and centrifugal speed is 3500-4500r/min, centrifugation time 15-25min.The high speed disintegrator energy short time makes pulverizing medicinal materials to breaking-wall cell, and in born of the same parents, active component or active component can fully come out, and directly touch extraction solvent, have improved recovery rate.Centrifugation apparatus is separated medicinal material residue and extract.
In above-mentioned control roxburgh anoectochilus terminal bud stem rot and the preparation method of soft rot medicine, in above-mentioned steps B, described gall nut and thunder godvine are first pulverized and at rear mistake 100 eye mesh screens of pulverizing before immersion, then soaking 30min decocts for the first time again, described first decoction first adopts intense fire that liquid is boiled, and boils rear slow fire boiling 30-50min; Described decoction again first adopts intense fire that liquid is boiled, and boils rear slow fire boiling 30-50min.Decocting time is that 30-50min can be fried by the active ingredient in medicinal material, intense fire Fast Heating, and slow fire guarantees effective components unchanged, it is for the active ingredient in medicinal material is fully fried that secondary decocts, and accomplishes to make the best use of everything, and does not waste medicinal material.
The control roxburgh anoectochilus terminal bud stem rot that the preparation method of above-mentioned control roxburgh anoectochilus terminal bud stem rot and soft rot medicine makes and soft rot medicine.The control efficiency of this control medicine is good, and nontoxic to human body, in application, environmental pollution is little.
Adopt roxburgh anoectochilus terminal bud stem rot and the Disease-Control for Soft-Rotting method of control roxburgh anoectochilus terminal bud stem rot and soft rot medicine, this method of preventing and treating comprises the steps: to get described the first treatment fluid and the second treatment fluid sprays roxburgh anoectochilus terminal bud cultivation matrix respectively or after mixing, and matrix is carried out disinfection; Get described the first treatment fluid and the second treatment fluid and respectively or after mixing roxburgh anoectochilus terminal bud is carried out to foliage-spray, early June and early July respectively spray once.In roxburgh anoectochilus terminal bud cultivation matrix, may contain pathogenic bacteria, spray and can eliminate a great propagating source, the cultivation of roxburgh anoectochilus terminal bud initial stage is had to larger help; The peak period of stem rot morbidity in annual May to July, soft rot is easily fallen ill at hot and humid environment, be onset peak period to July June, so, early June sprays and can play effectively preventing effect to roxburgh anoectochilus terminal bud stem rot and soft rot, early July sprays, if there is roxburgh anoectochilus terminal bud to occur that infecting of pathogenic bacteria can kill a large amount of pathogenic bacteria.
The medicine sterilizing mechanisms of this control roxburgh anoectochilus terminal bud stem rot and soft rot: the formula of preventing and treating roxburgh anoectochilus terminal bud stem rot and soft rot in the present invention comprises garlic, onion, gall nut, thunder godvine.The first treatment fluid that adopts cold formulation to obtain to comprise garlic and onion, the second treatment fluid that adopts hot decoction to obtain to comprise gall nut, thunder godvine, in order to prevent and treat roxburgh anoectochilus terminal bud stem rot and soft rot.Wherein in garlic and onion, sulfur-containing compound can suppress hypha,hyphae growth and the conidia germinations such as alternaric bacteria, Fusarium oxysporum, the pathogen of Botrytis cinerea, can effectively for roxburgh anoectochilus terminal bud stem rot, prevent and treat; In gall nut, in gallotannic acid and thunder godvine, the composition such as Triptolide can suppress the growths of the bacteriums such as staphylococcus, bacillus, Erwinia, can be effectively for roxburgh anoectochilus terminal bud Disease-Control for Soft-Rotting.
Compared with prior art, the medicine of this control roxburgh anoectochilus terminal bud stem rot and soft rot and formula thereof, preparation method and application's advantage is: formula is reasonable, successful, and cost is lower, and environmentally safe is nontoxic to human body; The preparation method who provides and the method for preventing and treating are simple to operate, are easy to grasp, reproducible, are applicable to applying on a large scale.
Embodiment
The preparation formula of this control roxburgh anoectochilus terminal bud stem rot and soft rot medicine comprises the component of following mass fraction: garlic 80-120 part; Onion 80-120 part; Gall nut 48-72 part; Thunder godvine 48-72 part.Preferably, the optimization component of this preparation formula is: 100 parts, garlic; 100 parts of onions; 60 parts of gall nut; 60 parts of thunder godvines.In the present invention, also can add kuh-seng, the matrine in kuh-seng is inhibited to soft rot pathogenic bacteria, and the fungicidal spectrum of matrine is wider simultaneously.The mass fraction summation of kuh-seng and thunder godvine is 48-72 part.Preferably, the mass fraction summation of kuh-seng and thunder godvine is 60 parts.When preparation control roxburgh anoectochilus terminal bud stem rot and soft rot medicine, the process of kuh-seng is identical with thunder godvine.
The preparation method of control roxburgh anoectochilus terminal bud stem rot and soft rot medicine comprises the steps:
The preparation of A, the first treatment fluid: get the water that garlic that parts by weight are 80-120 part, onion that parts by weight are 80-120 part and parts by weight are 320-480, then after garlic, onion and water being mixed, carry out pulverization process and obtain homogenate, homogenate is obtained after centrifugal treating to supernatant, obtain the first treatment fluid.Preferably, get parts by weight and be the water that the garlic of 100 parts, onion that parts by weight are 100 parts and parts by weight are 400, then after garlic, onion and water being mixed, carry out pulverization process and obtain homogenate, homogenate is obtained after centrifugal treating to supernatant, obtain the first treatment fluid.
The preparation of B, the second treatment fluid: getting gall nut and the parts by weight that parts by weight are 48-72 part is the thunder godvine of 48-72 part, is immersed in gall nut and thunder godvine in the water that parts by weight are 400-600, after immersion a period of time, decocts, and obtains first liquid; Then at the dregs of a decoction, adding parts by weight is the water of 200-400, again decocts, and obtains secondary liquid; First liquid and secondary liquid are mixed, be settled to the admixing medical solutions that parts by weight are 800-1200, obtain the second treatment fluid.Preferably, get parts by weight and be the thunder godvine that the gall nut of 60 parts and parts by weight are 60 parts, it is in 500 water that gall nut and thunder godvine are immersed in to parts by weight, decocts for the first time after soaking a period of time, obtains after filtering first liquid; Then at the dregs of a decoction, adding parts by weight is 300 water, again decocts, and obtains after filtering secondary liquid; First liquid and secondary liquid are mixed, be settled to parts by weight and be 1000 admixing medical solutions, obtain the second treatment fluid.
In above-mentioned steps A, described pulverization process is carried out in high speed disintegrator, and the rotating speed of high speed disintegrator is 4500-5500r/min; Described centrifugal treating is carried out in centrifugation apparatus, and centrifugal speed is 3500-4500r/min, centrifugation time 15-25min.In above-mentioned steps B, described gall nut and thunder godvine are first pulverized and at rear mistake 100 eye mesh screens of pulverizing before immersion, then soak 30min and decoct for the first time, described first decoction first adopts intense fire that liquid is boiled, and boils rear slow fire boiling 30-50min; Described decoction again first adopts intense fire that liquid is boiled, and boils rear slow fire boiling 30-50min.
Control roxburgh anoectochilus terminal bud stem rot and soft rot medicine that the preparation method of control roxburgh anoectochilus terminal bud stem rot and soft rot medicine makes.Adopt roxburgh anoectochilus terminal bud stem rot and the Disease-Control for Soft-Rotting method of control roxburgh anoectochilus terminal bud stem rot and soft rot medicine, this method of preventing and treating comprises the steps: to get described the first treatment fluid and the second treatment fluid sprays roxburgh anoectochilus terminal bud cultivation matrix respectively or after mixing, and matrix is carried out disinfection; Get described the first treatment fluid and the second treatment fluid and respectively or after mixing roxburgh anoectochilus terminal bud is carried out to foliage-spray, early June and early July respectively spray once.
Embodiment 1
1) implement place: Zhejiang Hangzhou Linan Zhejiang A & F University garden with hundreds of grasses
2) the enforcement time: in March, 2012-2012 year September
3) embodiment: 1. take 1000g garlic and 1000g onion and put into high speed disintegrator by solid-to-liquid ratio 1:2 (W/V), add 4000ml water, 5000r/min pulverizes, and gets its homogenate and be placed in the centrifugal 20min of centrifuge tube 4000r/min after pulverizing.Take out centrifuge tube, incline and supernatant, be the first treatment fluid, i.e. garlic and onion treatment fluid.2. take 600g gall nut and 600g thunder godvine, pulverized 100 eye mesh screens, add water 5000ml, decoct, boil rear 40min at a simmer, filter, the dregs of a decoction add water 3000ml, boil rear 40min at a simmer, filter; Merge 2 times liquid, be settled to 10000ml, be the second treatment fluid, be i.e. gall nut and thunder godvine treatment fluid.3. got above-mentioned garlic and onion treatment fluid (4000ml/ mu) and gall nut and thunder godvine treatment fluid (4000ml/ mu) on March 8 and respectively roxburgh anoectochilus terminal bud cultivation matrix is sprayed, take clear water as contrast, cultivation matrix is carried out disinfection; After transplanting, a minute three-wheel carries out dispenser, the first round is carried out foliage-spray for latter 10 days in transplanting, second takes turns with third round respectively at carrying out mid-May and mid-June foliage-spray, and every amount of application of taking turns is garlic and onion treatment fluid (3000ml/ mu) and gall nut and thunder godvine treatment fluid (3000ml/ mu).
4) result of implementation: treatment fluid group roxburgh anoectochilus terminal bud stem rot and the soft rot incidence of disease are 12.6%, clear water control group roxburgh anoectochilus terminal bud stem rot and the soft rot incidence of disease are 65.4%.
Embodiment 2
1) implement place: Zhejiang Hangzhou Linan Zhejiang A & F University garden with hundreds of grasses
2) the enforcement time: in March, 2013-2013 year September
3) embodiment: 1. take 1200g garlic and 1200g onion and put into high speed disintegrator by solid-to-liquid ratio 1:2 (W/V), add 4000ml water, 5000r/min pulverizes, and gets its homogenate and be placed in the centrifugal 20min of centrifuge tube 4000r/min after pulverizing.Take out centrifuge tube, incline and supernatant, be the first treatment fluid, i.e. garlic and onion treatment fluid.2. take 680g gall nut and 680g thunder godvine, pulverized 100 eye mesh screens, add water 5000ml, decoct, boil rear 40min at a simmer, filter, the dregs of a decoction add water 3000ml, boil rear 40min at a simmer, filter; Merge 2 times liquid, be settled to 10000ml, be the second treatment fluid, be i.e. gall nut and thunder godvine treatment fluid.3. got above-mentioned garlic and onion treatment fluid (4000ml/ mu) and gall nut and thunder godvine treatment fluid (4000ml/ mu) on March 12 and respectively roxburgh anoectochilus terminal bud cultivation matrix is sprayed, take clear water as contrast, cultivation matrix is carried out disinfection; After transplanting, a minute three-wheel carries out dispenser, the first round is carried out foliage-spray for latter 10 days in transplanting, second takes turns with third round respectively at carrying out mid-May and mid-June foliage-spray, and every amount of application of taking turns is garlic and onion treatment fluid (3000ml/ mu) and gall nut and thunder godvine treatment fluid (3000ml/ mu).
4) result of implementation: treatment fluid group roxburgh anoectochilus terminal bud stem rot and the soft rot incidence of disease are 14.1%, clear water control group roxburgh anoectochilus terminal bud stem rot and the soft rot incidence of disease are 69.3%.
Embodiment 3
1) implement place: HuangYang village, Huang Tan town, Wenzhou District of Zhejiang Province Wencheng County
2) the enforcement time: in March, 2013-2013 year September
3) embodiment: 1. take 1000g garlic and 1000g onion and put into high speed disintegrator by solid-to-liquid ratio 1:2 (W/V), add 4000ml water, 5000r/min pulverizes, and gets its homogenate and be placed in the centrifugal 25min of centrifuge tube 4500r/min after pulverizing.Take out centrifuge tube, incline and supernatant, be the first treatment fluid, i.e. garlic and onion treatment fluid.2. take 600g gall nut and 600g thunder godvine, pulverized 100 eye mesh screens, add water 5000ml, decoct, boil rear 40min at a simmer, filter, the dregs of a decoction add water 3000ml, boil rear 40min at a simmer, filter; Merge 2 times liquid, be settled to 10000ml, be the second treatment fluid, be i.e. gall nut and thunder godvine treatment fluid.3. getting above-mentioned garlic and onion treatment fluid (4500ml/ mu) and gall nut and thunder godvine treatment fluid (4500ml/ mu) on March 15 sprays roxburgh anoectochilus terminal bud cultivation matrix respectively, the 1000 times of liquid of carbendazol wettable powder of take are contrast, and cultivation matrix is carried out disinfection; After transplanting, a minute three-wheel carries out dispenser, the first round is carried out foliage-spray for latter 10 days in transplanting, second takes turns with third round respectively at carrying out mid-May and mid-June foliage-spray, and every amount of application of taking turns is garlic and onion treatment fluid (3500ml/ mu) and gall nut and thunder godvine treatment fluid (3500ml/ mu).
4) result of implementation: treatment fluid group roxburgh anoectochilus terminal bud stem rot and the soft rot incidence of disease are 15.7%, 1000 times of liquid control group roxburgh anoectochilus terminal bud stem rot of carbendazol wettable powder and the soft rot incidence of disease are 19.8%.The described treatment fluid control group incidence of disease refers to the data of the disease plant drawing after the first treatment fluid made from the present invention and the second treatment fluid are processed roxburgh anoectochilus terminal bud; The described clear water control group incidence of disease refers at same stage directly processes with clear water the data that roxburgh anoectochilus terminal bud calculates disease plant.
From the treatment fluid control group incidence of disease and these two groups of data of the clear water control group incidence of disease, can obviously find out that the present invention has great effect to the control of roxburgh anoectochilus terminal bud, it can kill a large amount of pathogenic bacteria, greatly reduces the incidence of disease of roxburgh anoectochilus terminal bud stem rot and soft rot; And avoided the Pesticide Residue of carbendazim.
Embodiment 4
This control medicine is that the first treatment fluid obtaining in above-described embodiment 1-3 and the second treatment fluid are combined and made with carrier chemically applicatory or chemical adjuvant.The first treatment fluid and the second treatment fluid got in embodiment 1 or 2 or 3 mix with appropriate water and auxiliary material respectively, make mixed liquor, and bottling sealing is standby.In order to improve effective component extraction rate, when preparation the first treatment fluid and the second treatment fluid, add respectively appropriate salt, and owing to having added salt, be conducive to extend the holding time of the first treatment fluid and the second treatment fluid.Wherein, the consumption of salt is limited roxburgh anoectochilus terminal bud is not grown to impact.
Embodiment 5
The preparation formula of this control roxburgh anoectochilus terminal bud stem rot and soft rot medicine comprises the component of following mass fraction: garlic 80-120 part; Onion 80-120 part; Gall nut 48-72 part; Thunder godvine 48-72 part; Separately add kuh-seng 48-72 part, capsicum 48-72 part, tobacco leaf 48-72 part, tea seed cake 48-72 part, any one in wormwood 48-72 part or multiple.Wherein, in gall nut and thunder godvine, add in kuh-seng, capsicum, tobacco leaf, tea seed cake, wormwood any one or multiple after, for the preparation of the second treatment fluid, the process of the second treatment fluid is identical with other embodiment.The amount of water of processing in the second treatment fluid processing procedure adds in the ratio in other embodiment.Due to added in kuh-seng, capsicum, tobacco leaf, tea seed cake, wormwood any one or multiple, not only improved the effect of control roxburgh anoectochilus terminal bud stem rot and soft rot, and possessed the function of deinsectization, be conducive to the growth of roxburgh anoectochilus terminal bud.And mentioned component is plant, realized the organic farming of roxburgh anoectochilus terminal bud.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various modifications or supplement or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Although herein more term, do not get rid of the possibility of using other term.Use these terms to be only used to describe more easily and explain essence of the present invention; They are construed to any additional restriction is all contrary with spirit of the present invention.
Claims (8)
1. a preparation formula of preventing and treating roxburgh anoectochilus terminal bud stem rot and soft rot medicine, is characterized in that, this preparation formula comprises the component of following mass fraction: garlic 80-120 part; Onion 80-120 part; Gall nut 48-72 part; Thunder godvine 48-72 part.
2. the preparation formula of control roxburgh anoectochilus terminal bud stem rot according to claim 1 and soft rot medicine, is characterized in that, the optimization component of this preparation formula is: 100 parts, garlic; 100 parts of onions; 60 parts of gall nut; 60 parts of thunder godvines.
3. a preparation method who prevents and treats roxburgh anoectochilus terminal bud stem rot and soft rot medicine, is characterized in that, this preparation method comprises the steps:
The preparation of A, the first treatment fluid: get the water that garlic that parts by weight are 80-120 part, onion that parts by weight are 80-120 part and parts by weight are 320-480, then after garlic, onion and water being mixed, carry out pulverization process and obtain homogenate, homogenate is obtained after centrifugal treating to supernatant, obtain the first treatment fluid;
The preparation of B, the second treatment fluid: getting gall nut and the parts by weight that parts by weight are 48-72 part is the thunder godvine of 48-72 part, is immersed in gall nut and thunder godvine in the water that parts by weight are 400-600, after immersion a period of time, decocts, and obtains first liquid; Then at the dregs of a decoction, adding parts by weight is the water of 200-400, again decocts, and obtains secondary liquid; First liquid and secondary liquid are mixed, be settled to the admixing medical solutions that parts by weight are 800-1200, obtain the second treatment fluid.
4. the preparation method of control roxburgh anoectochilus terminal bud stem rot according to claim 3 and soft rot medicine, is characterized in that, this preparation method comprises following Optimization Steps:
The preparation of A, the first treatment fluid: get parts by weight and be the water that the garlic of 100 parts, onion that parts by weight are 100 parts and parts by weight are 400, then after garlic, onion and water being mixed, carry out pulverization process and obtain homogenate, homogenate is obtained after centrifugal treating to supernatant, obtain the first treatment fluid;
The preparation of B, the second treatment fluid: get parts by weight and be the thunder godvine that the gall nut of 60 parts and parts by weight are 60 parts, it is in 500 water that gall nut and thunder godvine are immersed in to parts by weight, after soaking a period of time, decoct for the first time, obtain after filtering first liquid; Then at the dregs of a decoction, adding parts by weight is 300 water, again decocts, and obtains after filtering secondary liquid; First liquid and secondary liquid are mixed, be settled to parts by weight and be 1000 admixing medical solutions, obtain the second treatment fluid.
5. according to the preparation method of the control roxburgh anoectochilus terminal bud stem rot described in claim 3 or 4 and soft rot medicine, it is characterized in that, in above-mentioned steps A, described pulverization process is carried out in high speed disintegrator, and the rotating speed of high speed disintegrator is 4500-5500r/min; Described centrifugal treating is carried out in centrifugation apparatus, and centrifugal speed is 3500-4500r/min, centrifugation time 15-25min.
6. according to the preparation method of the control roxburgh anoectochilus terminal bud stem rot described in claim 3 or 4 and soft rot medicine, it is characterized in that, in above-mentioned steps B, described gall nut and thunder godvine are first pulverized and at rear mistake 100 eye mesh screens of pulverizing before immersion, then soaking 30min decocts for the first time again, described first decoction first adopts intense fire that liquid is boiled, and boils rear slow fire boiling 30-50min; Described decoction again first adopts intense fire that liquid is boiled, and boils rear slow fire boiling 30-50min.
7. the control roxburgh anoectochilus terminal bud stem rot making according to the preparation method of the control roxburgh anoectochilus terminal bud stem rot described in any one in claim 3-6 and soft rot medicine and soft rot medicine.
8. adopt roxburgh anoectochilus terminal bud stem rot and the Disease-Control for Soft-Rotting method of control roxburgh anoectochilus terminal bud stem rot claimed in claim 7 and soft rot medicine, it is characterized in that, this method of preventing and treating comprises the steps: to get described the first treatment fluid and the second treatment fluid sprays roxburgh anoectochilus terminal bud cultivation matrix respectively or after mixing, and matrix is carried out disinfection; Get described the first treatment fluid and the second treatment fluid and respectively or after mixing roxburgh anoectochilus terminal bud is carried out to foliage-spray, early June and early July respectively spray once.
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| CN201310601547.2A CN103689012A (en) | 2013-11-22 | 2013-11-22 | Drug for preventing and treating stem rot and soft rot of Anoectochilus roxburghii, and formula, preparation method and application thereof |
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