CN103651364A - Lentinan and fludioxonil-containing sterilization disease-resistant composition - Google Patents
Lentinan and fludioxonil-containing sterilization disease-resistant composition Download PDFInfo
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Abstract
The invention relates to a lentinan and fludioxonil-containing sterilization disease-resistant composition which comprises the following components by weight percent: 0.5-10% of lentinan and 0.5-30% of fludioxonil, wherein the weight ratio of lentinan to fludioxonil is (5:1)-(1:5), and (1:1)-(1:3) preferably. A flowable concentrate for seed coating and other dosage forms can be prepared through adding the known wetting agent, dispersing agent, film-forming agent, water and the like into the composition. The lentinan and fludioxonil-containing sterilization disease-resistant composition is reasonable in combination, remarkable in synergistic effect and better in sterilization and actual control efficiency, and can reduce the application amount of a pesticide and retard occurrence and development of resistance; seed treatment is performed before seed sowing, so that diseases of multiple crops including cotton and wheat during the seedling stage can be prevented and controlled, and the saline-alkaline tolerance capacity of crops can be remarkably improved; the lentinan and fludioxonil-containing sterilization disease-resistant composition has great application value and remarkable economic and social benefits.
Description
(1) technical field
The present invention relates to a kind of sterilization disease-resistant composition containing lentinan and fludioxonil, belong to agriculture chemical compounding technical field.
(2) background technology
Crops seedling stage disease large is the new problem in crop genetics in recent years, and plant growth is constituted a threat to, and has a strong impact on output.In agricultural production, due to the cause of disease in soil and the self-contained reasons such as cause of disease of crop seed, often cause the large generation of crops seedling stage disease, cause crop failure and affect quality that serious even total crop failure becomes a key constraints of agricultural production.People, in order to keep a full stand of seedings, strengthen the way of seeding quantity by employing, but this measure has not only been wasted seed but also can not really have been eliminated sprout term disease.
Lentinan is the fungi that produced by the Basidiomycetes Agaricaceae of the Pleurotaceae polysaccharide of birdsing of the same feather flock together, and take glucose as main, containing a small amount of mannose, fucose, galactose, wood sugar, arabinose etc.Lentinan is applied to field of medicaments the earliest, improves body immunity, anticancer, treatment malignant tumour and antiviral.But it has good anti-phytoviral activity equally at agriculture field, and be applied to produce.Lentinan also has disease-resistant, bacteriostatic activity, can produce and accumulate the disease resistance materials such as salicylic acid, protective plant protecting agent and chitinase by inducing plant.There are some researches show, lentinan not only can induce crop to produce good induction of resistance to disease, also phytopathogen is had to certain inhibitory action, and can promote crop seed to sprout and growth, and improves the anti-adversity ability of crop.
Fludioxonil (Fludioxonil) belongs to tagging property of pyroles bactericide, by suppressing the relevant transfer of glucose phosphorylated, and the mycelial growth of Antifungi, finally cause pathogen dead.Lasting period is long, and is difficult for and other bactericide generation cross resistance.Be widely used in various crop seed treatment, control kind passes and soil-borne disease bacterium, as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicillium bacterium.
Separately application lentinan prevents and to control crops seedling stage disease effect undesirable.Although fludioxonil has good broad spectrum activity and control efficiency to multiple sprout term disease, because its mechanism of action is single, and to have applied for many years, resistance increases year by year, and preventive effect reduces, and amount of application is also increasing separately, and use cost increases gradually.The relevant report that also there is no at present lentinan and fludioxonil mixed technology.
(3) summary of the invention
The object of the present invention is to provide a kind of sterilization disease-resistant composition containing lentinan and fludioxonil, solved and there is no at present this kind containing the sterilization disease-resistant composition of lentinan and fludioxonil and apply separately lentinan and prevent and control the undesirable problem of crops seedling stage disease effect.
A sterilization disease-resistant composition containing lentinan and fludioxonil, contains lentinan 0.5%～10% and fludioxonil 0.5%～30% by weight percentage; Wherein the weight ratio of lentinan and fludioxonil is 5:1～1:5.
Further, in described sterilization disease-resistant composition, adding the auxiliary agents such as known wetting dispersing agent, film forming agent and water adopts the known technology of those skilled in the art to can be made into the formulations such as suspension seed-coating agent.In this suspension seed-coating agent, each composition percentage by weight is: lentinan 0.5%～10%, fludioxonil 0.5%～30%, wetting dispersing agent 1%～20%, film forming agent 5%～10%, thickener 0.1%～3%, antifreezing agent 1%～5%, defoamer 0.1%～2%, colouring agent 0.1%～1%, all the other are water, above all the components percentage by weight summation is 100%, and wherein the weight ratio of lentinan and fludioxonil is 5:1～1:5.
The weight ratio of described lentinan and described fludioxonil is preferably 1:1～1:3.
The invention has the beneficial effects as follows that this kind is good containing the sterilization disease-resistant composition bactericidal effect of lentinan and fludioxonil, drug cost is low.
(4) embodiment
A kind of sterilization disease-resistant composition containing lentinan and fludioxonil of the present invention is in lentinan and fludioxonil, to add the known wetting agent of those skilled in that art, dispersant, the auxiliary agent such as film forming agent and water can be made into the formulations such as suspension seed-coating agent, in composition, each composition percentage by weight is: lentinan 0.5%～10%, fludioxonil 0.5%～30%, wetting dispersing agent 1%～20%, film forming agent 5%～10%, thickener 0.1%～3%, antifreezing agent 1%～5%, defoamer 0.1%～2%, colouring agent 0.1%～1%, all the other are water, above all the components percentage by weight summation is 100%, wherein the weight ratio of lentinan and fludioxonil is 5:1～1:5.Preferably the weight ratio of lentinan and described fludioxonil is 1:1～1:3.Another object of the present invention is to provide the application of above-mentioned composition on the sprout term disease of the various crop such as control cotton, wheat and maize.
The processing technology of the suspension seed-coating agent of above-mentioned sterilization disease-resistant composition, adopts known method preparation, is method conventional in formulations of pesticide processing technology.This compound synergic effect is remarkable, bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, to crop safety, for preventing and treating the sprout term disease of the various crop such as cotton, wheat and maize, and there is the Salt And Alkali Tolerance ability that promotes seed germination and growth of seedling and raising crop seedling.Composition component of the present invention is reasonable, and synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, to crop safety, and has the Salt And Alkali Tolerance ability that promotes seed germination and growth of seedling and improve crop seedling.
Below by experiment, the sterilization disease-resistant composition of lentinan of the present invention and fludioxonil is described:
Embodiment 1 Toxicity Determination:
Select representational cotton rhizoctonia solani (Rhizoctonia solani K ü hn) and rhizoctonia cerealis (Rhizoctonia cerealis), measure respectively lentinan, fludioxonil and the indoor virulence of the two different proportioning combination to these two kinds of pathogens, and calculated co-toxicity coefficient (CTC).Concrete grammar is:
Test method is with reference to the Plating in the agricultural industry criteria NY/T1156.2-2006 > > of the < < People's Republic of China (PRC).For single dose and each Mixed Pharmacy, according to preliminary experiment result, in causing the concentration range of pathogen inhibiting rate 20%-80%, according to geometric progression, set respectively 5 variable concentrations gradients, will be for examination pathogen 26 ℃ of preculture 3d on PDA medium, with the card punch of diameter 7mm, near beating on the same circumference of colony edge, getting bacterium cake, be inoculated into respectively on the PDA culture medium flat plate that contains above-mentioned 5 different drug concentrations, every ware a slice, every concentration is processed and is repeated 4 times, take and does not add medicament as contrast.Then culture dish is placed in 26 ℃ of insulating boxs and cultivates, cotton rhizoctonia solani is cultivated 2d(days), rhizoctonia cerealis is cultivated 3d, adopt right-angled intersection method to measure the colony growth diameter that each is processed, get its mean value, calculate the percentage that each chemicals treatment suppresses mycelial growth, by mycelial growth, suppress the linear regression analysis between probit value and drug concentration logarithm value, obtain each medicament to concentration (EC in effective inhibition of bacterial strain
50be worth), and adopt the abundant method of Sun Yun to calculate the co-toxicity coefficient (CTC) of mixture.Wherein: CTC >=120 show as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action, and as shown in table 1 is lentinan indoor co-toxicity experiment table to cotton rhizoctonia solani mixed with fludioxonil.
Table 1
As shown in table 2 is lentinan indoor co-toxicity experiment table to rhizoctonia cerealis mixed with fludioxonil.
Table 2
Toxicity Determination result shows: lentinan take with fludioxonil weight ratio as 1:1～1:3 mixed higher to cotton rhizoctonia solani and rhizoctonia cerealis virulence, synergistic effect is remarkable.
Embodiment 2 field control cotton seedling blights:
Test is located at respectively Tai’an, Shandong Province Shandong Agricultural University south school district Experimental Base and carry out in suburb, Zibo City, Shandong Province, and school district, Tai’an Shandong Agricultural University south Experimental Base soil property is sandy loam, middle fertility; Experimental field, suburb, Zibo City soil property is loam, slightly saline and alkaline, middle fertility; Each is cultivation condition uniformity experimental field.For studying thing, be cotton, kind is that Shandong cotton is ground No. 28.Reagent agent is self-control 10% lentinan fludioxonil suspension seed-coating agent, preparation method is: get lentinan 2.5%, fludioxonil 7.5%, YUS-207K0.5%, YUS-FS30004%, polyvinyl alcohol 8%, xanthans 0.1%, sodium bentonite 1.2%, propane diols 3%, n-octyl alcohol 0.5%, rhodamine toner 1.2%, it is 100% that distilled water adds to each composition percentage by weight summation.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, uses sand mill sand milling, until the grain fineness of suspension makes 10% lentinan fludioxonil suspension seed-coating agent after reaching requirement.Contrast medicament is commercially available 25g/L fludioxonil suspension seed-coating agent, by Switzerland Xian Zhengda crop protection Co., Ltd, is produced.
Designing 4 tests processes: (1) 10% lentinan fludioxonil suspension seed-coating agent 15g active ingredient dosage/100kg seed; (2) 10% lentinan fludioxonil suspension seed-coating agent 7.5g active ingredient dosage/100kg seeds; (3) 25g/L fludioxonil suspension seed-coating agent 15g active ingredient dosage/100kg seed; (4) Dressing (seed that does not add any medicament) is not blank group; Every processing repeats for 4 times, Gong16Ge community, and random district group is arranged.Each treatment agent is added to suitable quantity of water dilution evenly according to aequum, then with sprayer, liquid is all sprayed onto on load weighted seed, mix on spray limit, limit, and liquid is fully absorbed, and then dries sowing.Sowing time is respectively April 25 (Tai'an) in 2013 and April 20 (Zibo) in 2013, artificial dibbling.According to local field consumption and thickness of sowing, sow, every community selects 5 points quantitatively to sow according to five point samplings, and 100 cotton seeds of uniform broadcasting (2m), investigate for emergence rate.
Investigation and sampling method: at the number of emerging of each community fixed point sowing of blank cotton tidy seedlings output " Invest, Then Investigate " (after planting 10d left and right), and calculate emergence rate; 10d after emerging, 5 points of each community random choose, choose 20 strain cottons at every, carry out severity Scaling, record diseased plant numbers at different levels, calculate the incidence of disease and disease index, and calculate protection effect.Severity Scaling standard: 0 grade: strong seedling; 1 grade: slightly now scab or the slightly variable color of stem foot or root; 2 grades: 1/3 or 1/2 stem foot or root be scab or slightly variable color, rotten slightly now; 3 grades: full stem or root by scab around, variable color, rot, the contracting of hanging; 4 grades: cotton seedling is wilted withered.
Computational methods: diseased plant rate (%)=(morbidity strain number/investigate total strain number) * 100,
Disease index=[Σ (state of an illness rank * this rank diseased plant number)/(investigating total strain * 4)] * 100,
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100 are as shown in table 3 is lentinan field control effect table to cotton seedling blight mixed with fludioxonil:
Table 3
Field test results shows, 10% lentinan fludioxonil suspension seed-coating agent has good control efficiency to cotton seedling blight, and to the germination of cotton seeds with emerge and have obvious facilitation, emergence rate is apparently higher than contrast and contrast chemicals treatment.After every double centner seed is dressed seed by 15g and 7.5g effective ingredient consumption, to the preventive effect of cotton seedling blight all with fludioxonil single dose (being 25g/L fludioxonil suspension seed-coating agent) 15g active ingredient dosage/100kg seed treatment quite or be better than fludioxonil single dose (table 3).Duration of test is also observed and is found, 10% lentinan fludioxonil suspension seed-coating agent can significantly improve the germination of cotton seeds in saline land and emerge, and significantly improves the Salt And Alkali Tolerance ability of cotton seedling.
Embodiment 3 field control wheat sharp eyespots:
Test is located at the Li Jia village, Daiyue District, Tai’an, Shandong Province wheat paddock to be carried out, and experimental field preceding crop is corn, and soil property is loam, middle fertility, experimental field cultivation condition uniformity.For studying thing, be wheat, kind is mountain agriculture 14.Reagent agent is self-control 10% lentinan fludioxonil suspension seed-coating agent (preparation method is the same), and contrast medicament is commercially available 25g/L fludioxonil suspension seed-coating agent, by Switzerland Xian Zhengda crop protection Co., Ltd, is produced.Designing 4 tests processes: (1) 10% lentinan fludioxonil suspension seed-coating agent 15g active ingredient dosage/100kg seed; (2) 10% lentinan fludioxonil suspension seed-coating agent 7.5g active ingredient dosage/100kg seeds; (3) 25g/L fludioxonil suspension seed-coating agent 15g active ingredient dosage/100kg seed; (4) Dressing (seed that does not add any medicament) is not blank group.Every processing repeats for 4 times, Gong16Ge community, and random district group is arranged.1 day prior to seeding, mu seeding quantity and community area weighed each community sowing quantity and put into plastic sack or glassware routinely; By test Treatment Design, with the required medicament of graduated cylinder accurate measuring, after being watered than 1:50 by medicine (medicine+water) kind, liquid being poured in plastic sack or glassware and fully rocked, until seed is evenly stained with liquid again; Finally the seed numbering after dressing is dried, wait to broadcast.Sowing time is on September 25th, 2012, and sowing Shi Mei community selects 5 points to carry out artificial seeding according to five point samplings, and 100 Wheat Species of uniform broadcasting, investigate for emergence rate.Investigation and sampling method: wheat rough leaf is considered as emerging while exposing earth's surface 2cm, after the wheat tidy seedlings output of check plot, the number of emerging of each investigation fixed point sowing division, community, calculates emergence rate.At wheat during jointing stage, 5 points of each community random choose, choose 100 strain wheat wheat seedings at every, carry out severity Scaling, record diseased plant numbers at different levels, calculate the incidence of disease and disease index, and calculate protection effect.
Severity Scaling standard: 0 grade: anosis; 1 grade: 1st, 2 leaf sheath morbidities, cane is without scab; 2 grades: 3rd, 4 leaf sheath morbidities, cane is without scab; 3 grades: 1st, 2 leaf sheath morbidities, scab is less than 1/2 around cane; 4 grades: 3rd, 4 leaf sheath morbidities, scab is greater than 1/2 around cane; 5 grades: 5th, 6 leaf sheaths morbidities or scab were around cane one week; 6 grades: whole strain is withered or occur withered booting, withered dead ears.
Computational methods:
Diseased plant rate (%)=(morbidity strain number/investigate total strain number) * 100,
Disease index=[Σ (state of an illness rank * this rank diseased plant number)/(investigating total strain * 6)] * 100,
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100, as shown in table 4 is lentinan field control effect table to wheat sharp eyespot mixed with fludioxonil:
Table 4
Field test results shows, 10% lentinan fludioxonil suspension seed-coating agent has good control efficiency to wheat sharp eyespot, and to the germination of wheat seed with emerge and there is obvious facilitation, adopt after 10% lentinan fludioxonil suspension seed-coating agent seed dressing, emergence rate is apparently higher than fludioxonil single dose (being 25g/L fludioxonil suspension seed-coating agent) with without chemicals treatment.After every double centner seed is dressed seed by 15g and 7.5g effective ingredient consumption, to the preventive effect of wheat sharp eyespot all with fludioxonil single dose 15g active ingredient dosage/100kg seed treatment quite or be better than as above table 4 of fludioxonil single dose.
By above test, show, composition scheme of the present invention, its activity and bactericidal effect have significant synergies, control efficiency for the sprout term diseases of the crops such as cotton, wheat is obviously better than single dose, drug cost is low, be difficult for developing immunity to drugs, to crop safety, and there is the Salt And Alkali Tolerance ability that promotes seed germination and growth of seedling and improve crop seedling, there is larger promotional value.
Enumerate the specific embodiment of preparing suspension seed-coating agent below:
Embodiment 1: lentinan 2.5%, fludioxonil 0.5%, YUS-207K0.5%, YUS-FS30001%, polyvinyl alcohol 5%, xanthans 0.1%, propane diols 1%, n-octyl alcohol 0.1%, rhodamine toner 0.1%, all the other are distilled water, and above all the components percentage by weight summation is 100%.
Embodiment 2: lentinan 0.5%, fludioxonil 0.5%, YUS-207K0.2%, YUS-FS30000.8%, polyvinyl alcohol 5%, xanthans 0.2%, sodium bentonite 2.8%, propane diols 5%, n-octyl alcohol 0.1%, rhodamine toner 0.1%, all the other are distilled water, and above all the components percentage by weight summation is 100%.
Embodiment 3: lentinan 0.5%, fludioxonil 1.5%, YUS-FS30001%, polyvinyl alcohol 5%, xanthans 0.1%, propane diols 1%, n-octyl alcohol 0.1%, rhodamine toner 0.1%, all the other are distilled water, and above all the components percentage by weight summation is 100%.
Embodiment 4: lentinan 0.7, fludioxonil 2.8%, YUS-207K1%, YUS-FS30009%, polyvinyl alcohol 7%, xanthans 0.1%, sodium bentonite 0.9%, propane diols 3%, n-octyl alcohol 1%, rhodamine toner 0.5%, all the other are distilled water, and above all the components percentage by weight summation is 100%.
Embodiment 5: lentinan 10%, fludioxonil 30%, YUS-207K2%, YUS-FS300018%, polyvinyl alcohol 10%, xanthans 0.1%, propane diols 1%, n-octyl alcohol 2%, rhodamine toner 1%, all the other are distilled water, and above all the components percentage by weight summation is 100%.
Claims (5)
1. containing a sterilization disease-resistant composition for lentinan and fludioxonil, it is characterized in that containing lentinan 0.5%～10% and fludioxonil 0.5%～30% by weight percentage; Wherein the weight ratio of lentinan and fludioxonil is 5:1～1:5.
2. a kind of sterilization disease-resistant composition containing lentinan and fludioxonil as claimed in claim 1, the weight ratio that it is characterized in that described lentinan and fludioxonil is 1:1～1:3.
3. in sterilization disease-resistant composition as claimed in claim 1, add suspension seed-coating agent prepared by known auxiliary agent; In described suspension seed-coating agent, each composition percentage by weight is: lentinan 0.5%～10%, fludioxonil 0.5%～30%, wetting dispersing agent 1%～20%, film forming agent 5%～10%, thickener 0.1%～3%, antifreezing agent 1%～5%, defoamer 0.1%～2%, colouring agent 0.1%～1%, all the other are water, and above all the components percentage by weight summation is 100%.
4. the application of sterilization disease-resistant composition as claimed in claim 1 in control cotton seedling blight or wheat sharp eyespot.
5. the application of sterilization disease-resistant composition as claimed in claim 1 in improving cotton seedling Salt And Alkali Tolerance ability.
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Cited By (4)
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| CN106719769A (en) * | 2016-11-28 | 2017-05-31 | 山东农业大学 | A kind of disease pest containing lentinan, Difenoconazole and thiacloprid is simultaneous to control composition pesticide |
| CN107494710A (en) * | 2017-09-06 | 2017-12-22 | 山东省果树研究所 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
| CN114711130A (en) * | 2022-05-26 | 2022-07-08 | 山西农业大学 | Method for improving saline-alkali resistance of wheat by quinoa polysaccharide |
| CN115104492A (en) * | 2022-03-14 | 2022-09-27 | 浙江省农业科学院 | Simple and easy cultivation method for rice seed buds with zero pollution discharge |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719769A (en) * | 2016-11-28 | 2017-05-31 | 山东农业大学 | A kind of disease pest containing lentinan, Difenoconazole and thiacloprid is simultaneous to control composition pesticide |
| CN107494710A (en) * | 2017-09-06 | 2017-12-22 | 山东省果树研究所 | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease |
| CN115104492A (en) * | 2022-03-14 | 2022-09-27 | 浙江省农业科学院 | Simple and easy cultivation method for rice seed buds with zero pollution discharge |
| CN115104492B (en) * | 2022-03-14 | 2024-08-13 | 浙江省农业科学院 | Rice seedling bud zero-pollution-discharge light and simple cultivation method |
| CN114711130A (en) * | 2022-05-26 | 2022-07-08 | 山西农业大学 | Method for improving saline-alkali resistance of wheat by quinoa polysaccharide |
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