CN103636400B - Autologous rapid propagation method for Changbai Mountain cacalia firma kom - Google Patents
Autologous rapid propagation method for Changbai Mountain cacalia firma kom Download PDFInfo
- Publication number
- CN103636400B CN103636400B CN201310721875.6A CN201310721875A CN103636400B CN 103636400 B CN103636400 B CN 103636400B CN 201310721875 A CN201310721875 A CN 201310721875A CN 103636400 B CN103636400 B CN 103636400B
- Authority
- CN
- China
- Prior art keywords
- circular hole
- great
- days
- kom
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an autologous rapid propagation method for Changbai Mountain cacalia firma kom and belongs to the technical field of plant cultivation. The method comprises the steps as follows: (1) round holes are formed in leaves of the cacalia firma kom by a hole puncher; (2) veratrum oxysepalum turcz., pulsatilla cernua Bercht.et opiz. and epimedium koreanum nakai are hit into juice and mixed uniformly in a volume ratio of 2:2:1, and yellow clay is added and stirred to prepare slurry; (3) the round holes in leaf veins of the cacalia firma kom are coated with the prepared slurry, and filter paper is bonded to upper and lower surfaces of the round holes so as to keep the slurry humid; and (4) adventitious roots grow on edges of the round holes in the leaf veins of the cacalia firma kom after 15-20 days, adventitious buds are divided after 30-40 days, and after new plants growing in positions of the round holes of the leaves are higher than 1 cm, the plants are sheared by scissors and planted in soil. The method is simple to operate, little in effort, low in cost, high in yield, shorter in time, rapid in seedling emergence, higher in efficiency, good in profit and good in popularization and application prospect. By the aid of the method, the technical problem about plant propagation of the cacalia firma kom is solved.
Description
Technical field
The invention belongs to field of plant growing technology, particularly relate to a kind of plant cultivation method utilizing Changbai Mountain great Ye wild carraway blade breeding seedling.
Background technology
Great Ye wild carraway (Cacalia furma Kom.), another name great Ye rabbit umbrella, be the peculiar wild plant in Changbai Mountain, this plant distributions area stenosis, under being only distributed in the broad leaved forest of subregion, Changbai Mountain height above sea level 500 ~ 1000 meters of fertile soils.Field investigation premenstruum (premenstrua) and introduction and cultivation, find that this plant field is extremely low from numerous ability, in recent years the increasing forest zone common people order about by economic interests in addition, and start large-area collection great Ye wild carraway, its natural resources and forest zone vegetation are subject to heavy damage just day by day.
The medical value that great Ye wild carraway tool is high, can be used as medicinal plant and develops.Wild carraway extract has antitumor, bactericidal action, and its volatile oil and chemical composition are also the important component making biopesticide.Direct Shiqi young stem and leaf can detoxify and invigorate blood circulation, and swelling and pain relieving, cures mainly and dispel rheumatism.
Great Ye wild carraway basal leaf sheet is very large, and diameter can reach 1m.Because of the very large picture umbrella of its blade, therefore named rabbit umbrella.This Shade Tolerance is strong, is suitable for doing Cover Plant in the Park.
Patents in the middle of prior art also has no open.By " State Intellectual Property Office's patent search system " retrieval, not about the patent disclosure utilizing great Ye wild carraway self propagation seedling.
By the system retrieval keyword " wild carraway " such as " CNKI ", " general information is tieed up in Chongqing ", the artificial cultivation that there is no wild carraway is delivered with breeding correlative theses.The research of current wild carraway platymiscium also only concentrates on its medical value, all manually introduces a fine variety, cultivates and study on reproduction.
Keyword " wild carraway ", retrieves and belongs to related article not of the same race 8 sections together:
1, wild carraway belongs to study of pharmacy progress: " Agriculture of Anhui science " the 7th phase author: Jia Xu in 2011, high, Gong Jiang, Li Na, Hou Xiaoyi, Ni Shifeng, Zhao Ting, Lu Feng, Chen Qianliang, mechanism: Northwest University's Life Science College.
2, micro-wave digestion-FAAS to measure in Cacalia tangutica 8 kinds of micro-content: " spectrographic laboratory " the 6th phase author: Zhao Xiaohui in 2010, Zhang Xingwang, Mei Lijuan, Shao's Yun, Tao Yanduo, mechanism: Northwest Plateau-organisms Research Inst. of Chinese Academy of Sciences.
3, the research of honeysuckle China wild carraway chemical composition: " CHINA JOURNAL OF CHINESE MATERIA MEDICA " the 8th phase author: Lan Xiaocong, military hypo, Wang Wenshu in 2010, mechanism: Central University for Nationalities's life and environmental science institute.
4, plumage splits the research of magnificent wild carraway root chemical composition: " SWIC " originates: " Xianyang Normal University's journal " the 6th phase author: He Yangqing in 2007, Ma Zhanying, Yang Qian, high dawn, mechanism: applied chemistry system of Xi'an University of Technology.
5, the chemical composition analysis of Cacalia tangutica volatile oil and antibacterial activity research: " Wuhan University Journal: Edition " the 2nd phase author in 2007: Yang Yang, Zhu Shunying, Tang Lifei, Liu Deqing, Huang Jinghua, Zou Guolin, mechanism: Wuhan University's Life Science College/virology National Key Laboratory.
6, the two kinds of active components be separated from magnificent wild carraway are to the insecticidal activity of housefly: " insect journal " the 1st phase author: Zhou Lijuan in 2006, Huang Jiguang, Xu Hanhong, Wu Renhai, mechanism: Agricultural University Of South China; Agricultural chemicals and key lab of the chemical biology Ministry of Education; Botanical pesticide research center, south China.
7, the research of Mount Taibai wild carraway chemical composition: " Lanzhou University's journal: natural science edition " the 2nd phase author in 2003: Wang Pengfei, Yang Chao, Jia Zhongjian, mechanism: Chemistry &. Chemical Engineering College, Lanzhou Univ..
8, magnificent wild carraway genus and wild carraway belong to the caryogram of 4 Plants: " Yunnan plant research " the 4th phase author: Liu Jianquan in 2000, mechanism: Northwest Plateau-organisms Research Inst. of Chinese Academy of Sciences
Contrast other plant blade carries out the method for breeding, and is all cut from former plant by blade to carry out cuttage or tissue cultures.But great Ye wild carraway plant leaf is less, the raw big leaf's slice of base is very large, only has 1 piece, and about 50cm ~ 100cm is long, and blade is thinner, wilts very soon after cutting, and is difficult to carry out conventional leaf and inserts breeding.
Summary of the invention
Technical problem to be solved by this invention is: few for great Ye wild carraway blade, and the feature that blade is very large, the invention provides one not need blade to cut from former plant, but allow its still normal growth when carry out adventive root and adventitious bud inducing, form the method for new plant.
The autologous rapid breeding method of Changbai Mountain great Ye wild carraway, is characterized in that: comprise the following steps
Step one, with card punch on the blade master pulse of great Ye wild carraway Cacalia firma Kom or one-level lateral vein is beaten the circular hole that diameter is 0.5cm ~ 1.0cm, and the distance >2cm between circular hole;
Producing of step 2, plant mud, get fresh rhizome and root of Sharpsepal Falsehellebore (Veratrum oxysepalumTurcz.), pasque flower (Pulsatilla cernua Bercht.et Opiz.), korean epimedium herb (Epimedium koreanum Nakai) branches and leaves break into juice with juicer respectively, mix by the volume ratio of 2:2:1, adding yellow clay stirs into thick, obtained plant mud;
Step 3, the plant mud obtained in step 2 is coated on the circular hole of great Ye wild carraway vein, and the upper and lower surface of circular hole is bonded at filter paper, and filter paper diameter > Circularhole diameter, spray water on filter paper with watering can, keep plant mud to be in the dampness that humidity is 20% ~ 40% always;
The dampness of step 4, maintenance step 3 plant mud 15 days ~ 20 days, adventive root can be grown in the edge of great Ye wild carraway vein circular hole, continue to be cultured to 30 days ~ 40 days, each circular hole punishment dissolves 1 ~ 3 indefinite buds, when the new plant that blade circular hole director goes out grows tall to more than 1cm, it is cut with scissors and is planted in soil.
The dampness of maintenance step 3 plant mud 45 days ~ 60 days, adventive root can be grown in the edge of great Ye wild carraway vein circular hole, and adventive root length >=3cm, each circular hole punishment dissolves indefinite bud, and indefinite bud length >=1.5cm, the new plant now blade circular hole director gone out is cut with scissors and is planted in soil.
Adopt 5 pieces, great Ye wild carraway blade, obtain seedling 50 strain ~ 80 strain.
By above-mentioned design, the present invention can bring following beneficial effect: the invention solves great Ye wild carraway for a long time and be difficult to as other plant, and the method for blade cuttage can be adopted to carry out the technical barrier of breeding.Because great Ye wild carraway plant leaf is less, the raw big leaf's slice of base is very large, only has 1 piece, and about 50cm ~ 100cm is long, and blade is thinner, wilts very soon after cutting, so be difficult to carry out conventional blade cottage propagation.The technical barrier of this plant breeding, all annoyings the breeding field of great Ye wild carraway for a long time always.
The present invention, by plant mud coating process, solves this technical barrier cleverly.Namely first on great Ye wild carraway blade master pulse or one-level lateral vein, circular hole is got with card punch.Secondly fresh rhizome and root of Sharpsepal Falsehellebore, pasque flower, korean epimedium herb branches and leaves juicer are broken into juice, add yellow clay and stir into and thickly make plant mud.Again obtained plant mud is coated on the circular hole of great Ye wild carraway vein, and is bonded at circular hole upper and lower surface with the filter paper more bigger than circular hole.Spray water on filter paper through conventional watering can, to keep plant mud moist.Finally to after 15 days ~ 20 days, fibrous root can be grown in the edge of great Ye wild carraway vein circular hole, after 30 days ~ 40 days, can indefinite bud be differentiated.When the new plant that blade circular hole director goes out grows tall to more than 1cm, it is cut with scissors and is planted in soil.
The present invention's recruitment simple to operate is few, output with low cost is high, the time shorter emerge fast, comparatively high yield is good for efficiency, has good popularizing application prospect.
Embodiment
Embodiment 1
I, on great Ye wild carraway (Cacalia firma Kom.) blade master pulse, make a call to the circular hole of a diameter 1.0cm every 4cm ~ 5cm with card punch.
II, get fresh rhizome and root of Sharpsepal Falsehellebore, pasque flower, korean epimedium herb branches and leaves break into juice with juicer respectively, mix by the volume ratio of 2:2:1, add yellow clay and stir into thick, make plant mud.
III, plant mud is coated on the circular hole of great Ye wild carraway vein, and is bonded at circular hole upper and lower surface with the filter paper more bigger than circular hole.Every day sprays water on filter paper with watering can, to keep plant mud moist.
IV, after 20 days, grow adventive root in the edge of great Ye wild carraway vein circular hole, after 38 days, each circular hole punishment dissolves 2 ~ 3 indefinite buds.After 50 days, adventive root grows to about 3cm, the about 1.5cm of indefinite bud, is cut by each new seedling be planted in soil with scissors.
Through statistics, for test great Ye wild carraway basal leaf sheet 5 pieces, obtain seedling 76 strain altogether.
Embodiment 2
I, on great Ye wild carraway (Cacalia firma Kom.) blade 1 grade of lateral vein, make a call to the circular hole of a diameter 0.6cm every 3cm ~ 4cm with card punch.
II, get fresh rhizome and root of Sharpsepal Falsehellebore (Veratrum oxysepalum Turcz.), pasque flower (Pulsatilla cernua Bercht.et Opiz.), korean epimedium herb (Epimediumkoreanum Nakai) branches and leaves break into juice with juicer respectively, mix by the volume ratio of 2:2:1, adding yellow clay stirs into thick, makes plant mud.
III, obtained plant mud is coated on the circular hole of great Ye wild carraway vein, and is bonded at circular hole upper and lower surface with the filter paper more bigger than circular hole.Every day sprays water on filter paper with watering can, to keep plant mud moist.
IV, after 17 days, grow adventive root in the edge of great Ye wild carraway vein circular hole, after 32 days, each circular hole punishment dissolves 1 ~ 3 indefinite buds.After 45 days, adventive root grows to about 3cm, the about 1.5cm of indefinite bud, is cut by each new seedling be planted in soil with scissors.
Through statistics, for test great Ye wild carraway basal leaf sheet 5 pieces, obtain seedling 57 strain altogether.
Claims (3)
1. the autologous rapid breeding method of Changbai Mountain great Ye wild carraway, is characterized in that: comprise the following steps
Step one, with card punch on the blade master pulse of great Ye wild carraway (Cacalia furma Kom.) or one-level lateral vein is beaten the circular hole that diameter is 0.5cm ~ 1.0cm, and the distance >2cm between circular hole;
Producing of step 2, plant mud, gets fresh rhizome and root of Sharpsepal Falsehellebore, pasque flower, korean epimedium herb branches and leaves breaks into juice with juicer respectively, mix, add yellow clay and stir into thick, obtained plant mud by the volume ratio of 2:2:1;
Step 3, the plant mud obtained in step 2 is coated on the circular hole of great Ye wild carraway vein, and the upper and lower surface of circular hole is bonded at filter paper, and filter paper diameter > Circularhole diameter, spray water on filter paper with watering can, keep plant mud to be in the dampness that humidity is 20% ~ 40% always;
The dampness of step 4, maintenance step 3 plant mud 15 days ~ 20 days, adventive root can be grown in the edge of great Ye wild carraway vein circular hole, continue to be cultured to 30 days ~ 40 days, each circular hole punishment dissolves 1 ~ 3 indefinite buds, when the new plant that blade circular hole director goes out grows tall to more than 1cm, it is cut with scissors and is planted in soil.
2. the autologous rapid breeding method of Changbai Mountain great Ye wild carraway according to claim 1, it is characterized in that: the dampness of maintenance step 3 plant mud 45 days ~ 60 days, adventive root can be grown in the edge of great Ye wild carraway vein circular hole, and adventive root length >=3cm, each circular hole punishment dissolves indefinite bud, and indefinite bud length >=1.5cm, the new plant now blade circular hole director gone out is cut with scissors and is planted in soil.
3. the autologous rapid breeding method of Changbai Mountain great Ye wild carraway according to claim 1, is characterized in that: adopt 5 pieces, great Ye wild carraway blade, obtain seedling 50 strain ~ 80 strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310721875.6A CN103636400B (en) | 2013-12-24 | 2013-12-24 | Autologous rapid propagation method for Changbai Mountain cacalia firma kom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310721875.6A CN103636400B (en) | 2013-12-24 | 2013-12-24 | Autologous rapid propagation method for Changbai Mountain cacalia firma kom |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103636400A CN103636400A (en) | 2014-03-19 |
| CN103636400B true CN103636400B (en) | 2015-05-27 |
Family
ID=50241819
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310721875.6A Expired - Fee Related CN103636400B (en) | 2013-12-24 | 2013-12-24 | Autologous rapid propagation method for Changbai Mountain cacalia firma kom |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103636400B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101243774A (en) * | 2008-03-14 | 2008-08-20 | 成都大学 | Ligusticum wallichii tissue cultivation fast-propagating method with young leaflet tablet as explant |
| CN102124955A (en) * | 2011-01-31 | 2011-07-20 | 江苏农林职业技术学院 | Induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos |
| CN102715092A (en) * | 2012-07-06 | 2012-10-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches |
| CN102792889A (en) * | 2012-08-02 | 2012-11-28 | 四川大学 | Chuanminshen violaceum tissue culture rapid propagation technology |
| CN103385168A (en) * | 2013-07-01 | 2013-11-13 | 中南林业科技大学 | Method for regeneration plant of tung oil tree leaf |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000197423A (en) * | 1998-11-06 | 2000-07-18 | Japan Tobacco Inc | Culture and proliferation of solanaceous plant |
| KR20090084005A (en) * | 2008-01-31 | 2009-08-05 | 전북대학교산학협력단 | A method for propagation of bupleurum latissimum nakai in vitro |
-
2013
- 2013-12-24 CN CN201310721875.6A patent/CN103636400B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101243774A (en) * | 2008-03-14 | 2008-08-20 | 成都大学 | Ligusticum wallichii tissue cultivation fast-propagating method with young leaflet tablet as explant |
| CN102124955A (en) * | 2011-01-31 | 2011-07-20 | 江苏农林职业技术学院 | Induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos |
| CN102715092A (en) * | 2012-07-06 | 2012-10-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches |
| CN102792889A (en) * | 2012-08-02 | 2012-11-28 | 四川大学 | Chuanminshen violaceum tissue culture rapid propagation technology |
| CN103385168A (en) * | 2013-07-01 | 2013-11-13 | 中南林业科技大学 | Method for regeneration plant of tung oil tree leaf |
Non-Patent Citations (3)
| Title |
|---|
| 大叶蟹甲草不同萃取部位抗氧化活性研究;朱启光等;《人参研究》;20111231(第4期);第20－23页 * |
| 玫瑰海棠叶片培养与快速繁殖;孙美宏;《吉林农业》;20111231(第10期);第65页 * |
| 越橘组培快繁技术研究;马艳丽;《吉林林业科技》;20050131;第34卷(第1期);第3－5页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103636400A (en) | 2014-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102860209B (en) | High-efficiency lycoris and citrus interplanting method | |
| CN103283434B (en) | Method for cultivating polygonatum cyrtonema under phyllostachys edulis forest | |
| CN103688697A (en) | Cultivation method for puerarin | |
| CN103782787B (en) | GAP planting method of Lycoris aurea | |
| CN104137728A (en) | Three-dimensional planting method for traditional Chinese medicinal materials | |
| CN113141964B (en) | Planting method of long-acting carbon-neutralized forest in subtropical regions | |
| CN102939840A (en) | Cultivation method of mulberries in karst stony desertification mountain | |
| CN104186173A (en) | Acer truncatum selection and cultivation method | |
| CN103477844A (en) | Polygonum multiflorum planting method | |
| CN114916364B (en) | Method for cultivating large-diameter wood by mixed planting of larch and cyclobalanopsis glauca | |
| CN104737759A (en) | Scutellaria baicalensis Georgi cultivation method | |
| CN104938273A (en) | Method for interplanting gingers in orange garden | |
| CN107278599A (en) | A kind of CAULIS MARSDENIAE TENACISSIMAE implantation methods | |
| CN103210757A (en) | Method for culturing seedlings of polygonatum kingianum rhizome | |
| CN105875166A (en) | Amomum tsao-ko biomimetic seedling culturing method | |
| Zhang et al. | Prunus mira Koehne in Sichuan, China: recorded history as a medicine and food, modern applications, distribution, and ethnobotanical investigations | |
| CN103636400B (en) | Autologous rapid propagation method for Changbai Mountain cacalia firma kom | |
| CN104396687A (en) | Taxus chinensis cutting orchard construction method | |
| CN104962482B (en) | A kind of AM mycorrhizal fungi MW21 and its biocontrol agent and application | |
| CN105474978A (en) | Method for cultivating organic paris polyphylla in bamboo forest | |
| CN104094765B (en) | A kind of cultivation and collection method improving Taxus x media biomass | |
| CN102017862B (en) | Method for planting red kenmogi grass | |
| CN1846471A (en) | Artificial propagation method of Yunnan Chinese goldthread | |
| CN104429481A (en) | Cutting propagation method of Prunus cistena | |
| CN104137857A (en) | Preparation method for impatiens balsamina with pine wood nematode killing effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150527 Termination date: 20161224 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |