CN103599448B - Be used for the treatment of the Chinese medicine composition of infections chicken cloacal bursa - Google Patents
Be used for the treatment of the Chinese medicine composition of infections chicken cloacal bursa Download PDFInfo
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Abstract
The invention provides a kind of Chinese medicine composition being used for the treatment of infections chicken cloacal bursa, its crude drug comprises the Radix Astragali, the Radix Pulsatillae, Herba Agrimoniae, Herba Ecliptae, Herba Epimedii, Radix seu Herba Gei aleppici, Rhizoma Coptidis, Herba Hyperici Sampsonii, Radix Adenophorae, Galla Chinensis, Rhizoma Kaempferiae, Radix Et Caulis Acanthopanacis Senticosi, Bulbus Allii, Semen Myristicae, Semen Raphani, Fructus Bruceae, the Rhizoma Anemarrhenae, Herba Swertiae Mileensis, Fructus Crataegi, Radix Urenae Lobatae, Rhizoma Polygonati, Herba Mimosae Pudicae, Herba Schizonepetae, Folium Et Cacumen Murrayae and Semen Lablab Album.This Chinese medicine composition has significant effect in treatment infections chicken cloacal bursa, and has nontoxic, the advantage of drug resistance, drug residue free.
Description
Technical field
The present invention relates to technical field of Chinese medicine, particularly relate to a kind of Chinese medicine composition being used for the treatment of infections chicken cloacal bursa.
Background technology
Infectious bursal disease (IBDV) is also known as infectious bursal disease, that the one that caused by infectious bursa of Fabricius virus is acute, contact, immunosuppressive disease, be badly damaged as feature with fabricius bursa inflammation, necrosis, atrophy and fabricius bursa endolymph cell, clinical manifestation is lassitude, anorexia, indirect are suffered from diarrhoea, tremble and severe weakness.There is very strong pathogenic and higher mortality rate.Pathogenicity rate is 40 ~ 60% under normal circumstances, and mortality rate is generally 8 ~ 25%.
Primary disease mainly encroaches on the lymphoid tissues such as the humoral immunization maincenter organ fabricius bursa of chicken, because immunosuppressant causes secondary and concurrent other diseases, cause mortality rate obviously to raise, carry out major disaster to poultry industrial belt, being called as " acquired immune deficiency syndrome (AIDS) of chicken ", is one of topmost disease of current aviculture.
Primary disease incubation period is 2 ~ 3d, and having some cases to show as during morbidity increases without any the unexpected feed intake of symptom, within second day, sharply declines, occurs suddenly the chicken of off color in chicken group, and spread to full group very soon; Sick chicken down in spirits, fear of cold is weak, feather is fluffy and disorderly, drop-head closes order, two wings are sagging, squat on the ground, walk unstable, feed intake reduces, drinking-water increases, some is from anus pecking door, row's rice water sample or Ovum Gallus domesticus album sample feces, severe one dehydration, sleeping ground do not rise, extremely feeble, finally dead.Death mostly occurs at about 3 ~ 4d after being ill, presents typical " spike formula is dead ".Resistance to Anemia in Chicken of crossing is become thin, poor growth.
Chicken infectivity bursa of Fabricius virus mainly encroaches on the immune organ of chicken, causes immunosuppressant, and make disease chicken more other virulence factors of easy infection, the key of thus treating improves the immunologic function of chicken.
To infectious bursal disease, so far without effective western medicine.Someone uses dexamethasone and Norethisterone Compound, but is all mitigation symptoms and mitigate the disease, all can not etiological treatment or prevent infections.To early stage sick chicken, ideal effect can be obtained with high immunity yolk antibody or hyper-immune serum.But both are also subject to the impact of the conditions such as preparation, storage and transport, have that effect is uncertain maybe may propagate the shortcomings such as other diseases.Yolk antibody and serum are high protein material, nutritious, very easily polluted bacteria in production process, and with other cause of diseases, as egg-decreasing syndrome, avian leukosis, mycoplasma etc., may there is potential threat to aviculture.Produce antibody many commodities in use laying hen, antibody horizontal differs, and affects the titer level of yolk antibody; And, can antibody titer be reduced after being heated, must cryopreservation and transport, especially very easily putrid and deteriorated and can not use in summer.It contains a large amount of vitellin, fat etc., belongs to the coarse passive immunity preparation containing a large amount of foreign body, produces interference effect to normal immunity; The molecular weight of protein and fat etc. contained by it is all very large, and intramuscular injection is not only difficult to be absorbed, and also can cause local serious swelling, hemorrhage, downright bad and stress and anaphylaxis.The people such as ribavirin, Aminodiamantane, Moroxydine were once used for the treatment of infectious bursal disease with antiviral agents on veterinary clinic, but effect is uncertain, because it cures transplanting from people to come, lack scientific and normal, experimental data safely and effectively, not only control to bring adverse consequences to animal epidemic for viral disease, and affect the enforcement of National Animal control and prevention of disease policy.Therefore the Ministry of Agriculture is bright.
The traditional veterinary of motherland is of extensive knowledge and profound scholarship, and cure the disease more holistic and treating both the principal and secondary aspects of a disease, have unique curative effect by this disease for the treatment of by Chinese herbs.Primary disease is subordinate to the category of Chinese veterinarian's " pestilence ".Its etiology and pathogenesis is pyretic toxicity invasion and attack, and presents heat in both the exterior and interior, or pyretic toxicity spreads the three cloudy danger that internal organs cause and waits, and this disease directly enters edema caused by disorder of QI by warm heresy to compel to turn blood system and cause QI and blood two to broadcast, and intense heat in the blood, caused by the pyretic toxicity change of disease.The ultimate principle that natural Chinese medicinal herb prevents and treats this disease is strengthening vital QI to eliminate pathogenic factors, and namely ensure healthy physiological function and eliminate paathogenic factor to strengthen resistance against diseases, curative mechanism can be summarized as direct antivirus action and Immunity regulation two aspect to chicken.The present invention is exactly again the upper a kind of Chinese medicine composition being used for the treatment of infections chicken cloacal bursa proposed in this basis, and it has good therapeutic effect.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine composition being used for the treatment of infections chicken cloacal bursa, and it has significant effect in treatment infections chicken cloacal bursa, and has nontoxic, the advantage of drug resistance, drug residue free.
For solving the problems of the technologies described above, the invention provides a kind of Chinese medicine composition being used for the treatment of infections chicken cloacal bursa, its crude drug comprises the Radix Astragali, the Radix Pulsatillae, Herba Agrimoniae, Herba Ecliptae, Herba Epimedii, Radix seu Herba Gei aleppici, Rhizoma Coptidis, Herba Hyperici Sampsonii, Radix Adenophorae, Galla Chinensis, Rhizoma Kaempferiae, Radix Et Caulis Acanthopanacis Senticosi, Bulbus Allii, Semen Myristicae, Semen Raphani, Fructus Bruceae, the Rhizoma Anemarrhenae, Herba Swertiae Mileensis, Fructus Crataegi, Radix Urenae Lobatae, Rhizoma Polygonati, Herba Mimosae Pudicae, Herba Schizonepetae, Folium Et Cacumen Murrayae and Semen Lablab Album.
Wherein, in described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 35 weight portion ~ 45 weight portion, the Radix Pulsatillae 55 weight portion ~ 65 weight portion, Herba Agrimoniae 35 weight portion ~ 45 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Herba Epimedii 35 weight portion ~ 45 weight portion, Radix seu Herba Gei aleppici 25 weight portion ~ 35 weight portion, Rhizoma Coptidis 65 weight portion ~ 75 weight portion, Herba Hyperici Sampsonii 35 weight portion ~ 45 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion, Galla Chinensis 25 weight portion ~ 35 weight portion, Rhizoma Kaempferiae 35 weight portion ~ 45 weight portion, Radix Et Caulis Acanthopanacis Senticosi 20 weight portion ~ 30 weight portion, Bulbus Allii 25 weight portion ~ 35 weight portion, Semen Myristicae 30 weight portion ~ 40 weight portion, Semen Raphani 20 weight portion ~ 30 weight portion, Fructus Bruceae 35 weight portion ~ 45 weight portion, the Rhizoma Anemarrhenae 40 weight portion ~ 50 weight portion, Herba Swertiae Mileensis 20 weight portion ~ 30 weight portion, Fructus Crataegi 35 weight portion ~ 45 weight portion, Radix Urenae Lobatae 35 weight portion ~ 45 weight portion, Rhizoma Polygonati 20 weight portion ~ 30 weight portion, Herba Mimosae Pudicae 30 weight portion ~ 40 weight portion, Herba Schizonepetae 25 weight portion ~ 35 weight portion, Folium Et Cacumen Murrayae 30 weight portion ~ 40 weight portion and Semen Lablab Album 25 weight portion ~ 35 weight portion.
Wherein, in described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 38 weight portion ~ 42 weight portion, the Radix Pulsatillae 58 weight portion ~ 62 weight portion, Herba Agrimoniae 38 weight portion ~ 42 weight portion, Herba Ecliptae 28 weight portion ~ 32 weight portion, Herba Epimedii 38 weight portion ~ 42 weight portion, Radix seu Herba Gei aleppici 28 weight portion ~ 32 weight portion, Rhizoma Coptidis 68 weight portion ~ 72 weight portion, Herba Hyperici Sampsonii 38 weight portion ~ 42 weight portion, Radix Adenophorae 23 weight portion ~ 27 weight portion, Galla Chinensis 28 weight portion ~ 32 weight portion, Rhizoma Kaempferiae 38 weight portion ~ 42 weight portion, Radix Et Caulis Acanthopanacis Senticosi 23 weight portion ~ 27 weight portion, Bulbus Allii 28 weight portion ~ 32 weight portion, Semen Myristicae 33 weight portion ~ 37 weight portion, Semen Raphani 23 weight portion ~ 27 weight portion, Fructus Bruceae 38 weight portion ~ 42 weight portion, the Rhizoma Anemarrhenae 43 weight portion ~ 47 weight portion, Herba Swertiae Mileensis 23 weight portion ~ 27 weight portion, Fructus Crataegi 38 weight portion ~ 42 weight portion, Radix Urenae Lobatae 38 weight portion ~ 42 weight portion, Rhizoma Polygonati 23 weight portion ~ 27 weight portion, Herba Mimosae Pudicae 33 weight portion ~ 37 weight portion, Herba Schizonepetae 28 weight portion ~ 32 weight portion, Folium Et Cacumen Murrayae 33 weight portion ~ 37 weight portion and Semen Lablab Album 28 weight portion ~ 32 weight portion.
Wherein, in described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 40 weight portion, the Radix Pulsatillae 60 weight portion, Herba Agrimoniae 40 weight portion, Herba Ecliptae 30 weight portion, Herba Epimedii 40 weight portion, Radix seu Herba Gei aleppici 30 weight portion, Rhizoma Coptidis 70 weight portion, Herba Hyperici Sampsonii 40 weight portion, Radix Adenophorae 25 weight portion, Galla Chinensis 30 weight portion, Rhizoma Kaempferiae 40 weight portion, Radix Et Caulis Acanthopanacis Senticosi 25 weight portion, Bulbus Allii 30 weight portion, Semen Myristicae 35 weight portion, Semen Raphani 25 weight portion, Fructus Bruceae 40 weight portion, the Rhizoma Anemarrhenae 45 weight portion, Herba Swertiae Mileensis 25 weight portion, Fructus Crataegi 40 weight portion, Radix Urenae Lobatae 40 weight portion, Rhizoma Polygonati 25 weight portion, Herba Mimosae Pudicae 35 weight portion, Herba Schizonepetae 30 weight portion, Folium Et Cacumen Murrayae 35 weight portion and Semen Lablab Album 30 weight portion.
Present invention also offers the preparation method of above-mentioned Chinese medicine composition, be specially:
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtains Chinese medicine composition decocting liquid.
Present invention also offers a kind of Chinese medicine composition decocting liquid being used for the treatment of infections chicken cloacal bursa, its crude drug comprises the Radix Astragali, the Radix Pulsatillae, Herba Agrimoniae, Herba Ecliptae, Herba Epimedii, Radix seu Herba Gei aleppici, Rhizoma Coptidis, Herba Hyperici Sampsonii, Radix Adenophorae, Galla Chinensis, Rhizoma Kaempferiae, Radix Et Caulis Acanthopanacis Senticosi, Bulbus Allii, Semen Myristicae, Semen Raphani, Fructus Bruceae, the Rhizoma Anemarrhenae, Herba Swertiae Mileensis, Fructus Crataegi, Radix Urenae Lobatae, Rhizoma Polygonati, Herba Mimosae Pudicae, Herba Schizonepetae, Folium Et Cacumen Murrayae and Semen Lablab Album;
The preparation method of described decocting liquid is specially,
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtains Chinese medicine composition decocting liquid.
Wherein, in described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 35 weight portion ~ 45 weight portion, the Radix Pulsatillae 55 weight portion ~ 65 weight portion, Herba Agrimoniae 35 weight portion ~ 45 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Herba Epimedii 35 weight portion ~ 45 weight portion, Radix seu Herba Gei aleppici 25 weight portion ~ 35 weight portion, Rhizoma Coptidis 65 weight portion ~ 75 weight portion, Herba Hyperici Sampsonii 35 weight portion ~ 45 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion, Galla Chinensis 25 weight portion ~ 35 weight portion, Rhizoma Kaempferiae 35 weight portion ~ 45 weight portion, Radix Et Caulis Acanthopanacis Senticosi 20 weight portion ~ 30 weight portion, Bulbus Allii 25 weight portion ~ 35 weight portion, Semen Myristicae 30 weight portion ~ 40 weight portion, Semen Raphani 20 weight portion ~ 30 weight portion, Fructus Bruceae 35 weight portion ~ 45 weight portion, the Rhizoma Anemarrhenae 40 weight portion ~ 50 weight portion, Herba Swertiae Mileensis 20 weight portion ~ 30 weight portion, Fructus Crataegi 35 weight portion ~ 45 weight portion, Radix Urenae Lobatae 35 weight portion ~ 45 weight portion, Rhizoma Polygonati 20 weight portion ~ 30 weight portion, Herba Mimosae Pudicae 30 weight portion ~ 40 weight portion, Herba Schizonepetae 25 weight portion ~ 35 weight portion, Folium Et Cacumen Murrayae 30 weight portion ~ 40 weight portion and Semen Lablab Album 25 weight portion ~ 35 weight portion.
Beneficial effect of the present invention:
Chinese medicine composition provided by the invention has significant effect in treatment infections chicken cloacal bursa, and has nontoxic, the advantage of drug resistance, drug residue free.
Detailed description of the invention
The invention provides a kind of Chinese medicine composition being used for the treatment of infections chicken cloacal bursa, its crude drug comprises the Radix Astragali, the Radix Pulsatillae, Herba Agrimoniae, Herba Ecliptae, Herba Epimedii, Radix seu Herba Gei aleppici, Rhizoma Coptidis, Herba Hyperici Sampsonii, Radix Adenophorae, Galla Chinensis, Rhizoma Kaempferiae, Radix Et Caulis Acanthopanacis Senticosi, Bulbus Allii, Semen Myristicae, Semen Raphani, Fructus Bruceae, the Rhizoma Anemarrhenae, Herba Swertiae Mileensis, Fructus Crataegi, Radix Urenae Lobatae, Rhizoma Polygonati, Herba Mimosae Pudicae, Herba Schizonepetae, Folium Et Cacumen Murrayae and Semen Lablab Album.
Further preferably, described Chinese medicine composition is only made up of above-mentioned raw materials medicine.
Fang Zhong, the Radix Astragali, Radix seu Herba Gei aleppici can significantly improve cellular immunization and the humoral immunization ability of body, improve and adjust the immunologic function of body, effectively suppress infectious bursa of Fabricius virus infected chicken embryo fibroblast, the Radix Pulsatillae, Herba Agrimoniae has heat-clearing and toxic substances removing and can improve macrophages phagocytic capacity, Herba Ecliptae has heat clearing away YIN nourishing and has heart tonifying anastalsis, Herba Epimedii can significantly improve spleen antibody tormation level, strengthen the lymphocytic function of T, promote hypertrophy and the conversion of bone-marrow-derived lymphocyte, improve immunologic function, Rhizoma Coptidis energy antiinflammatory, antiallergic action, and there is very wide antimicrobial spectrum, have multiple pathogenic bacteria and influenza virus and comparatively pretend use, Herba Hyperici Sampsonii removing pathogenic heat from blood and toxic substance from the body, promoting blood flow to regulate menstruation, Radix Adenophorae can improve cellular immunization and non-specific immunity, suppress humoral immunization, there is the function of immunity moderation balance, Galla Chinensis convergence is astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, Radix Et Caulis Acanthopanacis Senticosi replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization, Bulbus Allii, Rhizoma Kaempferiae warming middle-JIAO helps digestion, circulation of qi promoting warming the stomach, Semen Myristicae, Semen Raphani warming middle-JIAO astringing intestine to stop diarrhea, promote qi circulation digestion promoting, Fructus Bruceae heat-clearing and toxic substances removing, the Rhizoma Anemarrhenae is nourshing Yin and drynsessmoistening prescription, clearing away lung-heat to relieve cough, Herba Swertiae Mileensis heat-clearing and toxic substances removing, dampness removing jaundice eliminating, hawthorn digesting stomach invigorating, Radix Urenae Lobatae expelling wind and removing dampness, heat-clearing and toxic substances removing, Rhizoma Polygonati enriching qi and yin, spleen invigorating lung moistening, Herba Mimosae Pudicae removing pathogenic heat from blood and toxic substance from the body, clearing away heat-damp and promoting diuresis, Herba Schizonepetae expelling pathogenic wind from the body surface, declare malicious rash, Folium Et Cacumen Murrayae promoting the circulation of QI to relieve pain, promoting blood circulation to remove blood stasis, Semen Lablab Album invigorating the spleen for eliminating dampness, with in relieve summer heat, all medicines share, play QI invigorating YIN nourishing altogether, heat-clearing and toxic substances removing, the merit of blood circulation promoting and blood stasis dispelling, the immunity of body can be regulated, by heat clearing away antipyretic and antidote functions and the subsidy to deficiency of vital QI, help, the effects such as promotion, can activate, promote, the cytokine that rise vigor is low, to adjust Molecular biological function, there is the effect strengthening animal body immunity.
Each crude drug pharmacology is as follows:
The Radix Astragali: sweet in the mouth, warm in nature, enter spleen, lung meridian, have tonification spleen soil, elevate a turnable ladder yang-energy, strengthening superficial resistance to stop perspiration, the effects such as holder skin ulcer granulation promoting, are mainly used in the weak anorexia and loose stool that deficiency of both the splenic and pulmonary QI is caused, shortness of breath and palpitation, the visceroptosis of sinking of QI of middle-JIAO, the metrorrhagia of QI failing to control blood is had blood in stool, chronic diarrhea proctoptosis; The spontaneous sweating of exterior deficiency, the cards such as insufficiency of vital energy and blood.The Radix Astragali can increase cellular immunization and humoral immune function, increases IgM, IgA in blood plasma, immunologic function is had to the effect of two-ways regulation.
The Radix Pulsatillae: bitter in the mouth, cold in nature, enters stomach, large intestine channel, has heat-clearing and toxic substances removing, the effect of eliminating pathogenic heat from blood to cure dysentery, and being mainly used in gastrointestinal has that damp and hot stagnant heating is suffered from abdominal pain, dysentery pus and blood, the cards such as tenesmus; To the hematodiarrhoea hematochezia better effects if of large intestine heat in blood, there is inhibitory action to dysentery bacterium, Bacillus typhi, bacillus subtilis, escherichia coli, Staphyloccocus aureus.
Herba Agrimoniae: latin name AgrimoniaeHerba, bitter in the mouth, puckery, property is put down, GUIXIN, Liver Channel, there is astringing to arrest bleeding, effect of dysentery relieving parasite killing, cure mainly various hemorrhage card, such as: haematemesis, hematuria, have blood in stool, metrorrhagia, spitting of blood, epistaxis, dysentery, impairment caused by overstrain takes off power, carbuncle, traumatic injury, the cards such as wound hemorrhage
Herba Ecliptae: have another name called Herba Ecliptae, ink grass, floods and droughts lotus, sweet in the mouth, acid, cold in nature, return liver, kidney channel, play liver and kidney tonifying, the effect of cooling blood for hemostasis, for having a dizzy spell caused by the hepatic and renal YIN deficiency, blurring of vision, soreness of the waist and knees; Be suitable for the deficiency of YIN and have hot person, be used for the treatment of the haematemesis of accumulated heat in stomach, the symptoms such as the hemoptysis of injury of blood vessel of the lung by heat, Herba Ecliptae has inhibitory action to staphylococcus aureus, Bacillus typhi, S. sonnei, bacillus pyocyaneus, can obvious enhancing non-specific immunity and cellular immune function.
Herba Epimedii: latin name EpimediiFolium, acrid in the mouth, sweet, warm in nature, enter liver, kidney channel, there is kidney-replenishing, bone and muscle strengthening, effect of wind-damp dispelling, cure mainly that impotence due to deficiency of the kidney, seminal emission premature ejaculation, waist knee joint flaccidity are soft, cold extremities with aversion to cold; Arthralgia due to cold-dampnes or spasm of the limbs numbness wait card, and Herba Epimedii is traditional kidney invigorating and YANG supporting Chinese medicine, is rich in flavone compound, has gonadotropic Effect, directly suppression bone resorption and promoting bone growing, the positive balance state making organism metabolism be in bone formation to be greater than bone resorption.
Radix seu Herba Gei aleppici: latin name GeiHerba, acrid in the mouth, hardship, property is put down, and return liver, spleen, lung meridian, have analgesia, blood pressure lowering, regulating menstruation, effect of expelling wind and removing dampness, cures mainly hypertension, dizziness headache, menoxenia, lower abdominal pain, leucorrhea, infantile convulsion, lumbago, skelalgia of rheumatism; Carbuncle furuncle and phyma poison is controlled in external, the cards such as traumatic injury.
Rhizoma Coptidis: bitter in the mouth, cold in nature, enter the heart, lung, gallbladder, stomach, large intestine channel, have heat clearing and damp drying, eliminating fire and detoxication, the effect of cooling blood for improving eyesight, be usually used in the fire for the treatment of removing pathogen in the heart warp, the dysphoria and insomnia that heart-fire is prosperous, and the high fever coma of epidemic febrile disease, the card such as vexed, thirsty.
Herba Hyperici Sampsonii: bitter in the mouth, pungent, cold in nature, return liver, spleen channel, there is removing pathogenic heat from blood and toxic substance from the body, promoting blood flow to regulate menstruation, effect of dispelling wind and removing obstruction in the collateral, cure mainly various hemorrhage, enteritis and dysentery, acute mastitis, carbuncle furunculosis, scald, venom, menoxenia, dysmenorrhea, leucorrhea, traumatic injury, rheumatic arthralgia, the cards such as aphtha.
Radix Adenophorae: sweet in the mouth, micro-hardship, cold nature, enter lung, stomach warp, there is nourishing the lung to arrest cough, the effect of nourishing the stomach to promote the production of body fluid, be mainly used in lung-dryness syndrome due to deficiency of YIN, dry cough few expectorant, chronic cough expectorant blood, the cards such as the few tongue of deficiency of stomach-Yin, mouthfeel parched throat, constipation, red tongue, cellular immunization and non-specific immunity can be improved, suppress humoral immunization, there is the function of immunity moderation balance.
Galla Chinensis: latin name GallaChinensis, sour in the mouth, puckery, cold in nature, return lung, large intestine, kidney channel, there is effect of astringent or styptic treatment for spontaneous sweating, the supplementing QI for promoting the production of body fluid of convergence, kidney calming, cure mainly: chronic cough of deficiency lung; Spontaneous sweating; Chronic dysentery chronic diarrhea; Proctoptosis; Seminal emission; Nebulousurine; Various hemorrhage; The cards such as carbuncle furuncle, Galla Chinensis energy enhancing body, to the defence capability of nonspecific stimulation, can strengthen the immunologic function of cell, has inhibitory action to this pathogenic bacterium, and can antiviral growth.
Rhizoma Kaempferiae: acrid in the mouth, warm in nature, returns stomach warp, has circulation of qi promoting warming middle-JIAO, the effect of the pain relieving that helps digestion, cure mainly chest and diaphragm distension, coldness and pain in the epigastrium, and diet such as not to disappear at the card.
Radix Et Caulis Acanthopanacis Senticosi: latin name AcanthopanacisSenticosiRadixetRhizomaseuCaulis, acrid in the mouth, hardship, micro-sweet, warm in nature, enter spleen, kidney, heart channel, there is replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization, effect of expelling wind and removing dampness, soft for anemofrigid-damp arthralgia, lumbocrural pain, muscles and bones flaccidity, be slow in action, the card such as body void is frail, traumatic injury, fracture, edema, beriberi, uncontrolled urination are itched.
Bulbus Allii: warm in nature, acrid in the mouth is put down; Enter spleen, stomach, lung meridian, have that warming middle-JIAO helps digestion, the stagnant gas of row, warming the spleen and stomach, removing food stagnancy, removing toxic substances, parasite killing effect, cure mainly eating accumulation, coldness and pain in the epigastrium, edema distension, have loose bowels, dysentery, malaria, pertussis, swollen ulcer drug, white bald tinea skin ulcer, snake bite and insect sting and the disease such as ancylostome, pinworm.
Semen Myristicae: latin name MyristicaeSemen, acrid in the mouth, hardship, warm in nature, enter spleen, stomach, large intestine channel, there is the effect of warming middle-JIAO astringing intestine to stop diarrhea, promote qi circulation digestion promoting, the card such as not disappear for diarrhea of deficiency type, cold-dysentery, abdominal distention, lack of appetite vomiting, dyspepsia.
Semen Raphani: property is put down, acrid in the mouth, sweet, returns lung, spleen, stomach warp, has the de-swelling that helps digestion, effect of lowering the adverse-rising QI to resolve phlegm, cures mainly retention of food and drink, abdominal distention, constipation, stagnant dysentery, expectorant stops up cards such as breathing with cough.
Fructus Bruceae: latin name BruceaeFructus, bitter in the mouth, cold in nature, return large intestine channel, Liver Channel, have heat-clearing and toxic substances removing, dysentery relieving, preventing the attack (or recurrence) of malaria, effect of corrosion wart, cures mainly dysentery, malaria; The cards such as external treatment wart, clavus.
The Rhizoma Anemarrhenae: latin name AnemarrhenaeRHizoma, bitter in the mouth, sweet, cold in nature, enter lung, stomach, kidney channel, have relieving restlessness of purging heat, the effect of nourshing Yin and drynsessmoistening prescription, clearing away lung-heat to relieve cough, cure mainly fever caused by exogenous pathogenic factors, high heat is irritated, lung-heat type cough, and interior-heat such as to be quenched one's thirst at the symptom; Its bitter cold matter is moistened, and property sedimentation can heat clearing away, can promote the production of body fluid again, kindly controls warm pathogenic factor and enters edema caused by disorder of QI, intenseness of heat impairment of body fluid; Also diabetes is used for the treatment of; Phlegm-heat obstructing in the lung and breathing heavily, xeropulmonary cough, expectorant is few unfavorable; Hyperactivity of fire caused by deficiency of YIN, the symptoms such as hyperactivity of deficient fire, the Rhizoma Anemarrhenae has inhibitory action to various bacteria such as dysentery bacterium, Bacillus typhi, escherichia coli, Bacillus proteus, staphylococcus, streptococcus.
Herba Swertiae Mileensis: bitter in the mouth, cold in nature, return liver, gallbladder, urinary bladder channel, have heat-clearing and toxic substances removing, effect of dampness removing jaundice eliminating, cures mainly jaundice due to damp-heat; The puckery pain of pyretic stranguria; Damp-heat dysentery; Leucorrhea with red and white discharge; Influenza; Malaria is generated heat; Acute gastritis; Acute pharyngolaryngitis; The cards such as acute tonsillitis.
Fructus Crataegi: sour in the mouth, sweet, slightly warm in nature, returns spleen, stomach, Liver Channel, has promoting digestion and invigorating the stomach, effect of circulation of qi promoting dissipating blood stasis, is mainly used to treat accumulation of food in the stomach and intes tine due to indigestion, has loose bowels, dysentery, Fructus Crataegi is the staple for the treatment of dyspepsia, the cards such as Fructus Crataegi can increase the secretion of peptic digestion enzyme, facilitating digestion.
Radix Urenae Lobatae: sweet in the mouth, light, property is put down, and enter the heart, lung meridian, have expelling wind and removing dampness, effect of heat-clearing and toxic substances removing, cures mainly cold, fever, rheumatic arthralgia, dysentery, edema, leucorrhea, spits blood, carbuncle, traumatic hemorrhage, venom, acute infantile convulsion, tetanus, asthma, the cards such as stomachache puerperal exposure to wind.
Rhizoma Polygonati: latin name PolygonatiRhizoma, sweet in the mouth, property is put down, enter lung, spleen, kidney channel, there is enriching qi and yin, spleen invigorating lung moistening, effect of enriching kidney essence, for taste deficiency of qi and yin, lack of appetite is unable, lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, quenching one's thirst of yin asthenia generating intrinsic heat, the card such as deficiency of kidney essence, impotence and seminal emission, dizziness and tinnitus, blurred vision dim eyesight.
Herba Mimosae Pudicae: sweet in the mouth, puckery, micro-hardship, cold nature, returns spleen, kidney two warp, have removing pathogenic heat from blood and toxic substance from the body, clearing away heat-damp and promoting diuresis, effect of tranquillizing and allaying excitement, cures mainly flu, infantile hyperpyrexia, bronchitis, hepatitis, gastritis, conjunctivitis, urinary system calculus, edema, hemoptysis, hematuria, neurasthenia, insomnia, sore swollen toxin, herpes zoster, the cards such as traumatic injury.
Herba Schizonepetae: acrid in the mouth, slightly warm in nature, enters lung, Liver Channel, has expelling pathogenic wind from the body surface, declares malicious rash, and effect for the treatment of blood disorders relieving convulsion cures mainly flu cold and heat, headache, eye itching, pharyngalgia, cough, measles, rubella, carbuncle skin ulcer, brigadier, spit blood, clot blood, has blood in stool, metrorrhagia, the card such as postpartum apoplexy, bruise.
Folium Et Cacumen Murrayae: acrid in the mouth, micro-hardship, warm in nature, return liver, stomach warp, has promoting the circulation of QI to relieve pain, and effect of promoting blood circulation to remove blood stasis, cures mainly stomachache, rheumatic arthralgia; External treatment is had a toothache, tumbling and swelling, the cards such as worm venom.
Semen Lablab Album: sweet in the mouth, slightly warm in nature, enters spleen, stomach warp, have invigorating the spleen for eliminating dampness and in the effect of relieving summer heat, for the indigestion and loss of appetite abdominal distention of weakness of the spleen and stomach, loose stool, aqueous-grainy diarrhea, the cards such as vomiting of feeling sick.
In described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 35 weight portion ~ 45 weight portion, the Radix Pulsatillae 55 weight portion ~ 65 weight portion, Herba Agrimoniae 35 weight portion ~ 45 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Herba Epimedii 35 weight portion ~ 45 weight portion, Radix seu Herba Gei aleppici 25 weight portion ~ 35 weight portion, Rhizoma Coptidis 65 weight portion ~ 75 weight portion, Herba Hyperici Sampsonii 35 weight portion ~ 45 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion, Galla Chinensis 25 weight portion ~ 35 weight portion, Rhizoma Kaempferiae 35 weight portion ~ 45 weight portion, Radix Et Caulis Acanthopanacis Senticosi 20 weight portion ~ 30 weight portion, Bulbus Allii 25 weight portion ~ 35 weight portion, Semen Myristicae 30 weight portion ~ 40 weight portion, Semen Raphani 20 weight portion ~ 30 weight portion, Fructus Bruceae 35 weight portion ~ 45 weight portion, the Rhizoma Anemarrhenae 40 weight portion ~ 50 weight portion, Herba Swertiae Mileensis 20 weight portion ~ 30 weight portion, Fructus Crataegi 35 weight portion ~ 45 weight portion, Radix Urenae Lobatae 35 weight portion ~ 45 weight portion, Rhizoma Polygonati 20 weight portion ~ 30 weight portion, Herba Mimosae Pudicae 30 weight portion ~ 40 weight portion, Herba Schizonepetae 25 weight portion ~ 35 weight portion, Folium Et Cacumen Murrayae 30 weight portion ~ 40 weight portion and Semen Lablab Album 25 weight portion ~ 35 weight portion.
Further preferably, in described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 38 weight portion ~ 42 weight portion, the Radix Pulsatillae 58 weight portion ~ 62 weight portion, Herba Agrimoniae 38 weight portion ~ 42 weight portion, Herba Ecliptae 28 weight portion ~ 32 weight portion, Herba Epimedii 38 weight portion ~ 42 weight portion, Radix seu Herba Gei aleppici 28 weight portion ~ 32 weight portion, Rhizoma Coptidis 68 weight portion ~ 72 weight portion, Herba Hyperici Sampsonii 38 weight portion ~ 42 weight portion, Radix Adenophorae 23 weight portion ~ 27 weight portion, Galla Chinensis 28 weight portion ~ 32 weight portion, Rhizoma Kaempferiae 38 weight portion ~ 42 weight portion, Radix Et Caulis Acanthopanacis Senticosi 23 weight portion ~ 27 weight portion, Bulbus Allii 28 weight portion ~ 32 weight portion, Semen Myristicae 33 weight portion ~ 37 weight portion, Semen Raphani 23 weight portion ~ 27 weight portion, Fructus Bruceae 38 weight portion ~ 42 weight portion, the Rhizoma Anemarrhenae 43 weight portion ~ 47 weight portion, Herba Swertiae Mileensis 23 weight portion ~ 27 weight portion, Fructus Crataegi 38 weight portion ~ 42 weight portion, Radix Urenae Lobatae 38 weight portion ~ 42 weight portion, Rhizoma Polygonati 23 weight portion ~ 27 weight portion, Herba Mimosae Pudicae 33 weight portion ~ 37 weight portion, Herba Schizonepetae 28 weight portion ~ 32 weight portion, Folium Et Cacumen Murrayae 33 weight portion ~ 37 weight portion and Semen Lablab Album 28 weight portion ~ 32 weight portion.
Most preferably, in described Chinese medicine composition, the weight portion of each crude drug is respectively the Radix Astragali 40 weight portion, the Radix Pulsatillae 60 weight portion, Herba Agrimoniae 40 weight portion, Herba Ecliptae 30 weight portion, Herba Epimedii 40 weight portion, Radix seu Herba Gei aleppici 30 weight portion, Rhizoma Coptidis 70 weight portion, Herba Hyperici Sampsonii 40 weight portion, Radix Adenophorae 25 weight portion, Galla Chinensis 30 weight portion, Rhizoma Kaempferiae 40 weight portion, Radix Et Caulis Acanthopanacis Senticosi 25 weight portion, Bulbus Allii 30 weight portion, Semen Myristicae 35 weight portion, Semen Raphani 25 weight portion, Fructus Bruceae 40 weight portion, the Rhizoma Anemarrhenae 45 weight portion, Herba Swertiae Mileensis 25 weight portion, Fructus Crataegi 40 weight portion, Radix Urenae Lobatae 40 weight portion, Rhizoma Polygonati 25 weight portion, Herba Mimosae Pudicae 35 weight portion, Herba Schizonepetae 30 weight portion, Folium Et Cacumen Murrayae 35 weight portion and Semen Lablab Album 30 weight portion.
Present invention also offers the preparation method of above-mentioned Chinese medicine composition, be specially:
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtains Chinese medicine composition decocting liquid.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 decocting liquid 1
By Radix Astragali 40g, Radix Pulsatillae 60g, Herba Agrimoniae 40g, Herba Ecliptae 30g, Herba Epimedii 40g, Radix seu Herba Gei aleppici 30g, Rhizoma Coptidis 70g, Herba Hyperici Sampsonii 40g, Radix Adenophorae 25g, Galla Chinensis 30g, Rhizoma Kaempferiae 40g, Radix Et Caulis Acanthopanacis Senticosi 25g, Bulbus Allii 30g, Semen Myristicae 35g, Semen Raphani 25g, Fructus Bruceae 40g, Rhizoma Anemarrhenae 45g, Herba Swertiae Mileensis 25g, Fructus Crataegi 40g, Radix Urenae Lobatae 40g, Rhizoma Polygonati 25g, Herba Mimosae Pudicae 35g, Herba Schizonepetae 30g, Folium Et Cacumen Murrayae 35g and Semen Lablab Album 30g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtain Chinese medicine composition decocting liquid 1.
Embodiment 2 decocting liquid 2
By Radix Astragali 38g, Radix Pulsatillae 58g, Herba Agrimoniae 38g, Herba Ecliptae 28g, Herba Epimedii 38g, Radix seu Herba Gei aleppici 28g, Rhizoma Coptidis 68g, Herba Hyperici Sampsonii 38g, Radix Adenophorae 23g, Galla Chinensis 28g, Rhizoma Kaempferiae 38g, Radix Et Caulis Acanthopanacis Senticosi 23g, Bulbus Allii 28g, Semen Myristicae 33g, Semen Raphani 23g, Fructus Bruceae 38g, Rhizoma Anemarrhenae 43g, Herba Swertiae Mileensis 23g, Fructus Crataegi 38g, Radix Urenae Lobatae 38g, Rhizoma Polygonati 23g, Herba Mimosae Pudicae 33g, Herba Schizonepetae 28g, Folium Et Cacumen Murrayae 33g and Semen Lablab Album 28g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtain Chinese medicine composition decocting liquid 2.
Embodiment 3 decocting liquid 3
By Radix Astragali 42g, Radix Pulsatillae 62g, Herba Agrimoniae 42g, Herba Ecliptae 32g, Herba Epimedii 42g, Radix seu Herba Gei aleppici 32g, Rhizoma Coptidis 72g, Herba Hyperici Sampsonii 42g, Radix Adenophorae 27g, Galla Chinensis 32g, Rhizoma Kaempferiae 42g, Radix Et Caulis Acanthopanacis Senticosi 27g, Bulbus Allii 32g, Semen Myristicae 37g, Semen Raphani 27g, Fructus Bruceae 42g, Rhizoma Anemarrhenae 47g, Herba Swertiae Mileensis 27g, Fructus Crataegi 42g, Radix Urenae Lobatae 42g, Rhizoma Polygonati 27g, Herba Mimosae Pudicae 37g, Herba Schizonepetae 32g, Folium Et Cacumen Murrayae 37g and Semen Lablab Album 32g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtain Chinese medicine composition decocting liquid 3.
Embodiment 4 toxicological experiment
Acute toxicity testing
Select Kunming mouse 20, female half and half, body weight (20+2) g, is provided by Qingdao City's Experimental Animal Center, and decocting liquid prepared by embodiment 1 is gavaged mice, through preliminary experiment, fails to detect the LD of oral administration
50, therefore survey its maximum tolerated dose, with the administration volume of 1.4ml/10g body weight, gavage 2 times/d, interval 6h, to observation post administration 7d, record animal dead and toxicity symptom, at the end of experiment, by all animals process, dissect, perusal each main organs lesion tissue situation, after gavaging 140g crude drug/kg for 2 times, animal activity, feed water inlet does not show any abnormalities, observe 7d animal dead, gross necropsy has no the obvious exception of main organs tissue, therefore, the maximum dosage-feeding of Chinese medicine granules is >140g crude drug/kg, this dosage is equivalent to 70 times of maximum oral dose 2g crude drug/kg clinical adult (by 60kg) every day.
Long term toxicity test
Select 40 male SD rats, weight (180 ± 20) g, thered is provided by Qingdao City's Experimental Animal Center, be divided into 2 groups at random, blank group and Chinese drug-treated group, often organize 20, blank group gives distilled water, decocting liquid prepared by embodiment 1 is gavaged rat, the amount of gavaging is 50g crude drug/kg, on every Mondays to gastric infusion 1 time in morning Saturday, and continuous 180d, administration volume is 2mL/100g body weight, and every day observes rat general state, sign, food ration, drinking-water and feces situation before administration and during administration.During administration 90d after last administration 24h, the extraction 6 of each group, male and female half and half; During 180d after last administration 24h, the extraction 6 of each group, male and female half and half; After drug withdrawal, 30d respectively organizes extraction 6, male and female half and half, anaesthetize with urethane, blood is got from abdominal aortic, each group is all killed and gets animal, checks hematology and blood parameters, after getting blood, amass wealth by heavy taxation at once core, liver,kidney,spleen, lung, adrenal gland, thyroid, thymus, testis, ovary, uterus, brain, the internal organs such as prostate weigh, and make pathological examination according to weight calculator official weight coefficient.
On inspection, during successive administration, the activity of animal, body weight, food ration, stool and hair luster degree no abnormality seen, animal has no dead, naked eyes have no obvious pathological changes, each group of rat peripheral hemogram checking is all in normal range, and physiochemical indice is all in normal range, and Chinese drug-treated group Rats Organs and Tissues coefficient does not have significant difference compared with blank group.
Embodiment 5 zoopery
Laboratory animal
Select public young 210 of the nonimmune Luo Man of 1 age in days, not vaccination is raised to 30 ages in days, and IBD is negative after testing.
Experimental drug
Decocting liquid 1 prepared by embodiment 1.
IBDV high immunity yolk antibody is provided by Qingdao Agricultural University, and antibody titer is 1: 128; IBDV standard positive serum is purchased from Qingdao Agricultural University.
Prepared by counteracting toxic substances chicken infectivity bursa of Fabricius virus
Get the fabricius bursa that field acquisition has typical cytopathic, smash multigelation to pieces 4 times with tissue mashing machine, the centrifugal 10min of 800r/min, get supernatant, recrement again with normal saline dilution, centrifugal, suct clear liquid, 4 times repeatedly, merge supernatant and make 1:4 homogenate ,-20 DEG C save backup.
Grouping and method
Chicken is divided into three groups at random, and often organize 70, A group is treatment by Chinese herbs group, and B group is IBDV high immunity yolk antibody treatment group, and C group is matched group.3 groups of chickens simultaneously with 0.2mL/ IBDV diluent collunarium, after clinical symptoms to appear, A group oral administered compound Chinese medicine 1.5mL/ only, 2 times/day; B group chicken muscle injection IBDV high immunity yolk antibody 1mL/ only; C group chicken injecting normal saline 1mL/ only, observes morbidity death and the pathological change of chicken.
Egg inoculation protection test
Virus liquid is made doubling dilution, inoculates instar chicken embryo on the 10th 10 through allantoic cavity, 0.2mL/; Herb liquid is done respectively 1: 2,1: 4 times of dilution, and different dilution factor medicinal liquid is mixed with appropriate virus liquid equal-volume for subsequent use respectively, 37 DEG C of effect 1h, inoculate each 10 of 10 age in days SPF Embryo Gallus domesticus through allantoic cavity respectively, 0.2mL/, do PBS contrast simultaneously, then 37.5 DEG C are continued hatching chicken embryo, every 12h shines egg 1 time, and records death condition, until 108h off-test.
Clinical treatment is tested
Select typical IBD morbidity chicken 800, IBD natural occurrence chicken group machine is divided into two groups, only, another only organizes oral Chinese medicine composition decocting liquid 1.5mL/ to one group of intramuscular injection IBDV high immunity yolk antibody 1mL/, 2 times/day.
Result
Manual therapeutic is tested
After counteracting toxic substances 24h, test chicken lassitude, stands transfixed to the ground, and feather is fluffy and disorderly, arranges white loose stool, and diet reduces or gives up absolutely.After medication, A group, the B group chicken state of an illness from the 2nd day starts to take a turn for the better, and within 3rd ~ 4 days, occurs dead chicken, the basic rehabilitation of the chicken survived after 5d; After the 2nd day, sb.'s illness took a turn for the worse there is symptom for c group, starts to occur death, within 3rd ~ 5 days, reaches peak mortality, the spontaneous recovery after the 6th day of not dead chicken.As can be seen from Table 1, Chinese medicine composition decocting liquid of the present invention is adopted to only have 6 final death, cure rate is 91.43%, B group has 16 death, cure rate is 77.14%, c group has 57 death, mortality rate be 81.43%, A group and B group significant difference (P<0.05), with C group difference more remarkable (P<0.01).
IBD result of the test treated by table 1
| Group | Quantity (only) | Morbidity number (only) | Mortality rate (%) | Cure rate (%) |
| A group | 70 | 70 | 8.57％ | 91.43％ |
| B group | 70 | 70 | 22.86％ | 77.14％ |
| C group | 70 | 70 | 81.43％ | 18.57％ |
Inspection is cutd open to dead chicken, nearly all chicken bursa serous coat edema and even glue peptone sample; Cloaca is dispersed in petechia, and thigh, chest muscle, lower limb flesh femoribus internus are hemorrhage; Pericardial effusion, coronary sulcus is hemorrhage; Renomegaly, the time case urate deposition of length is obvious; Glandular stomach has ecchymosis; The enlargement of fabricius bursa yellow-white, most congested, hemorrhage.The dead chicken of A group is hemorrhage comparatively light, especially gastrointestinal tract and fabricius bursa pathological changes not obvious.
Egg inoculation protection test, the results are shown in Table 2.
Table 2 suppresses chicken embryo death situation
| Processed group | Embryo Gallus domesticus number (individual) | Inoculum concentration (mL) | Dead germ rate (%) |
| IBDV+1：2 | 20 | 0.2 | 15 |
| IBDV+1：4 | 20 | 0.2 | 20 |
| IBDV virus liquid matched group | 20 | 0.2 | 85 |
| PBS matched group | 20 | 0.2 | 0 |
As can be seen from Table 2, Chinese drug-treated group dead germ rate is starkly lower than virus liquid matched group, P<0.01, shows that Chinese medicine composition decocting liquid provided by the invention can suppress to infect the death of IBDV Embryo Gallus domesticus, has good protective effect to Embryo Gallus domesticus.
Clinical Treatment Test, the results are shown in Table 3.
Table 3 pair IBD natural occurrence chicken group therapeutic effect
| Group | Treatment number (only) | Death toll (only) | Cure rate (%) |
| Chinese drug-treated group | 400 | 15 | 96.25 |
| Antibody group | 400 | 59 | 85.25 |
As can be seen from Table 3, the therapeutic effect of Chinese medicine composition decocting liquid of the present invention is better than antibody group.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (5)
1. be used for the treatment of a Chinese medicine composition for infections chicken cloacal bursa, it is characterized in that: crude drug is made up of the Radix Astragali, the Radix Pulsatillae, Herba Agrimoniae, Herba Ecliptae, Herba Epimedii, Radix seu Herba Gei aleppici, Rhizoma Coptidis, Herba Hyperici Sampsonii, Radix Adenophorae, Galla Chinensis, Rhizoma Kaempferiae, Radix Et Caulis Acanthopanacis Senticosi, Bulbus Allii, Semen Myristicae, Semen Raphani, Fructus Bruceae, the Rhizoma Anemarrhenae, Herba Swertiae Mileensis, Fructus Crataegi, Radix Urenae Lobatae, Rhizoma Polygonati, Herba Mimosae Pudicae, Herba Schizonepetae, Folium Et Cacumen Murrayae and Semen Lablab Album;
The weight portion of each crude drug is respectively the Radix Astragali 35 weight portion ~ 45 weight portion, the Radix Pulsatillae 55 weight portion ~ 65 weight portion, Herba Agrimoniae 35 weight portion ~ 45 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Herba Epimedii 35 weight portion ~ 45 weight portion, Radix seu Herba Gei aleppici 25 weight portion ~ 35 weight portion, Rhizoma Coptidis 65 weight portion ~ 75 weight portion, Herba Hyperici Sampsonii 35 weight portion ~ 45 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion, Galla Chinensis 25 weight portion ~ 35 weight portion, Rhizoma Kaempferiae 35 weight portion ~ 45 weight portion, Radix Et Caulis Acanthopanacis Senticosi 20 weight portion ~ 30 weight portion, Bulbus Allii 25 weight portion ~ 35 weight portion, Semen Myristicae 30 weight portion ~ 40 weight portion, Semen Raphani 20 weight portion ~ 30 weight portion, Fructus Bruceae 35 weight portion ~ 45 weight portion, the Rhizoma Anemarrhenae 40 weight portion ~ 50 weight portion, Herba Swertiae Mileensis 20 weight portion ~ 30 weight portion, Fructus Crataegi 35 weight portion ~ 45 weight portion, Radix Urenae Lobatae 35 weight portion ~ 45 weight portion, Rhizoma Polygonati 20 weight portion ~ 30 weight portion, Herba Mimosae Pudicae 30 weight portion ~ 40 weight portion, Herba Schizonepetae 25 weight portion ~ 35 weight portion, Folium Et Cacumen Murrayae 30 weight portion ~ 40 weight portion and Semen Lablab Album 25 weight portion ~ 35 weight portion.
2. Chinese medicine composition as claimed in claim 1, is characterized in that: the weight portion of each crude drug is respectively the Radix Astragali 38 weight portion ~ 42 weight portion, the Radix Pulsatillae 58 weight portion ~ 62 weight portion, Herba Agrimoniae 38 weight portion ~ 42 weight portion, Herba Ecliptae 28 weight portion ~ 32 weight portion, Herba Epimedii 38 weight portion ~ 42 weight portion, Radix seu Herba Gei aleppici 28 weight portion ~ 32 weight portion, Rhizoma Coptidis 68 weight portion ~ 72 weight portion, Herba Hyperici Sampsonii 38 weight portion ~ 42 weight portion, Radix Adenophorae 23 weight portion ~ 27 weight portion, Galla Chinensis 28 weight portion ~ 32 weight portion, Rhizoma Kaempferiae 38 weight portion ~ 42 weight portion, Radix Et Caulis Acanthopanacis Senticosi 23 weight portion ~ 27 weight portion, Bulbus Allii 28 weight portion ~ 32 weight portion, Semen Myristicae 33 weight portion ~ 37 weight portion, Semen Raphani 23 weight portion ~ 27 weight portion, Fructus Bruceae 38 weight portion ~ 42 weight portion, the Rhizoma Anemarrhenae 43 weight portion ~ 47 weight portion, Herba Swertiae Mileensis 23 weight portion ~ 27 weight portion, Fructus Crataegi 38 weight portion ~ 42 weight portion, Radix Urenae Lobatae 38 weight portion ~ 42 weight portion, Rhizoma Polygonati 23 weight portion ~ 27 weight portion, Herba Mimosae Pudicae 33 weight portion ~ 37 weight portion, Herba Schizonepetae 28 weight portion ~ 32 weight portion, Folium Et Cacumen Murrayae 33 weight portion ~ 37 weight portion and Semen Lablab Album 28 weight portion ~ 32 weight portion.
3. Chinese medicine composition as claimed in claim 1 or 2, it is characterized in that: the weight portion of each crude drug is respectively the Radix Astragali 40 weight portion, the Radix Pulsatillae 60 weight portion, Herba Agrimoniae 40 weight portion, Herba Ecliptae 30 weight portion, Herba Epimedii 40 weight portion, Radix seu Herba Gei aleppici 30 weight portion, Rhizoma Coptidis 70 weight portion, Herba Hyperici Sampsonii 40 weight portion, Radix Adenophorae 25 weight portion, Galla Chinensis 30 weight portion, Rhizoma Kaempferiae 40 weight portion, Radix Et Caulis Acanthopanacis Senticosi 25 weight portion, Bulbus Allii 30 weight portion, Semen Myristicae 35 weight portion, Semen Raphani 25 weight portion, Fructus Bruceae 40 weight portion, the Rhizoma Anemarrhenae 45 weight portion, Herba Swertiae Mileensis 25 weight portion, Fructus Crataegi 40 weight portion, Radix Urenae Lobatae 40 weight portion, Rhizoma Polygonati 25 weight portion, Herba Mimosae Pudicae 35 weight portion, Herba Schizonepetae 30 weight portion, Folium Et Cacumen Murrayae 35 weight portion and Semen Lablab Album 30 weight portion.
4. the preparation method of Chinese medicine composition described in claim 1 or 2, is characterized in that:
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtains Chinese medicine composition decocting liquid.
5. be used for the treatment of a Chinese medicine composition decocting liquid for infections chicken cloacal bursa, it is characterized in that: crude drug is made up of the Radix Astragali, the Radix Pulsatillae, Herba Agrimoniae, Herba Ecliptae, Herba Epimedii, Radix seu Herba Gei aleppici, Rhizoma Coptidis, Herba Hyperici Sampsonii, Radix Adenophorae, Galla Chinensis, Rhizoma Kaempferiae, Radix Et Caulis Acanthopanacis Senticosi, Bulbus Allii, Semen Myristicae, Semen Raphani, Fructus Bruceae, the Rhizoma Anemarrhenae, Herba Swertiae Mileensis, Fructus Crataegi, Radix Urenae Lobatae, Rhizoma Polygonati, Herba Mimosae Pudicae, Herba Schizonepetae, Folium Et Cacumen Murrayae and Semen Lablab Album;
In described Chinese medicine composition decoction liquor, the weight portion of each crude drug is respectively the Radix Astragali 35 weight portion ~ 45 weight portion, the Radix Pulsatillae 55 weight portion ~ 65 weight portion, Herba Agrimoniae 35 weight portion ~ 45 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Herba Epimedii 35 weight portion ~ 45 weight portion, Radix seu Herba Gei aleppici 25 weight portion ~ 35 weight portion, Rhizoma Coptidis 65 weight portion ~ 75 weight portion, Herba Hyperici Sampsonii 35 weight portion ~ 45 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion, Galla Chinensis 25 weight portion ~ 35 weight portion, Rhizoma Kaempferiae 35 weight portion ~ 45 weight portion, Radix Et Caulis Acanthopanacis Senticosi 20 weight portion ~ 30 weight portion, Bulbus Allii 25 weight portion ~ 35 weight portion, Semen Myristicae 30 weight portion ~ 40 weight portion, Semen Raphani 20 weight portion ~ 30 weight portion, Fructus Bruceae 35 weight portion ~ 45 weight portion, the Rhizoma Anemarrhenae 40 weight portion ~ 50 weight portion, Herba Swertiae Mileensis 20 weight portion ~ 30 weight portion, Fructus Crataegi 35 weight portion ~ 45 weight portion, Radix Urenae Lobatae 35 weight portion ~ 45 weight portion, Rhizoma Polygonati 20 weight portion ~ 30 weight portion, Herba Mimosae Pudicae 30 weight portion ~ 40 weight portion, Herba Schizonepetae 25 weight portion ~ 35 weight portion, Folium Et Cacumen Murrayae 30 weight portion ~ 40 weight portion and Semen Lablab Album 25 weight portion ~ 35 weight portion,
The preparation method of described decocting liquid is specially,
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml, obtains Chinese medicine composition decocting liquid.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1401370A (en) * | 2002-09-27 | 2003-03-12 | 长春市动物药厂 | Extracted herbal medicine for treating chicken infectious bursa of Fabricius |
| CN1899498A (en) * | 2006-07-05 | 2007-01-24 | 河北农业大学 | Medicine for preventing and controlling chicken infectious fabricius bursa disease |
| CN101406637A (en) * | 2008-11-18 | 2009-04-15 | 卢海军 | Medicament for treating infectious bursal disease of chicken |
| CN101683452A (en) * | 2008-09-25 | 2010-03-31 | 天津生机集团股份有限公司 | Animal Chinese medicine oral liquid for preventing and treating infectious bursal disease of fowl and preparation method thereof |
| CN102488847A (en) * | 2011-12-20 | 2012-06-13 | 青岛农业大学 | Traditional Chinese medicine composition for treating chicken infectious bursal disease as well as preparation method and application thereof |
-
2013
- 2013-10-23 CN CN201310500556.2A patent/CN103599448B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1401370A (en) * | 2002-09-27 | 2003-03-12 | 长春市动物药厂 | Extracted herbal medicine for treating chicken infectious bursa of Fabricius |
| CN1899498A (en) * | 2006-07-05 | 2007-01-24 | 河北农业大学 | Medicine for preventing and controlling chicken infectious fabricius bursa disease |
| CN101683452A (en) * | 2008-09-25 | 2010-03-31 | 天津生机集团股份有限公司 | Animal Chinese medicine oral liquid for preventing and treating infectious bursal disease of fowl and preparation method thereof |
| CN101406637A (en) * | 2008-11-18 | 2009-04-15 | 卢海军 | Medicament for treating infectious bursal disease of chicken |
| CN102488847A (en) * | 2011-12-20 | 2012-06-13 | 青岛农业大学 | Traditional Chinese medicine composition for treating chicken infectious bursal disease as well as preparation method and application thereof |
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