CN103539548A - Method for producing bio-fertilizer - Google Patents
Method for producing bio-fertilizer Download PDFInfo
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- CN103539548A CN103539548A CN201310489855.0A CN201310489855A CN103539548A CN 103539548 A CN103539548 A CN 103539548A CN 201310489855 A CN201310489855 A CN 201310489855A CN 103539548 A CN103539548 A CN 103539548A
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Abstract
The invention aims to provide a method for producing a bio-fertilizer. The method comprises the following steps of: fermenting Bacillus subtilis, Pseudomonas aeruginosa, Lactobacillus plantarum and Bacillus thuringiensis by adopting high-density liquid respectively, and preparing a super-thick microbial agent of which the spore content reaches 1011-1012CFU/g through a fermentation process after-treatment technology; then weighing 30-100 parts of the microbial agent, 100-300 parts of thoroughly-decomposed straw powder, 100-150 parts of urea, 50-120 parts of potassium chloride and 80-150 parts of diatomite according to proportions, uniformly mixing in a stirring machine, performing extrusion granulation, and performing air drying to obtain the bio-fertilizer. The method is simple in operation, less in process, suitable for the existing factory equipment and good for wide popularization.
Description
Technical field
The present invention relates to fertilizer production technology field, be specifically related to a kind of method of producing bio-feritlizer.
Background technology
The bio-feritlizer of narrow sense, is by microbial life activity, makes farm crop obtain the goods of specific fertilizer effect, is also referred to as Inoculant or bacterial manure, and itself, containing nutritive element, can not replace chemical fertilizer.Broadly bio-feritlizer is both to have contained the required nutritive element of crop, contains again the goods of microorganism, be biological, organic and inorganic combination it can replace chemical fertilizer, provide crop growthing development required all kinds of nutritive elements.The extensive application of chemical fertilizer and agricultural chemicals pros and cons depositing for the mankind, for promoting what is beneficial and abolish what is harmful, scientist has proposed " ecological agriculture ", progressively realizes and in farmland, uses or do not use less chemical fertilizer and chemical insecticide, and use organic biofertilizer and adopt microbial process prevention and elimination of disease and pests
Bio-feritlizer can be increase soil fertility, this is its Main Function, as various vinelandii fertilizer, can increase the sources of nitrogen in soil, P and K decomposing bacterial manure material can be dissolved out the phosphorus of insoluble in soil, potassium, increases the source of Soil Phosphorus (P), potassium (K) element.In addition, bio-feritlizer can also promote the growth of crop, improves the quality of agricultural-food.During various bio-fertilizers are manured into soil, can produce different tethelin, stimulate the growth of crop, as " 5406 " actinomycetes bio-fertilizer, not only have the effect of the strong bacterium of antagonism pathogenic bacteria diseases prevention, can also secretory cell mitogen promote the growth of crop.The bio-fertilizer of Mycophyta is not only assisting there is being very strong effect aspect the mineral elements such as Crop phosphorus, zinc and copper, strengthens in addition the water suction, water conservation of crop to improve the effect of crop drought resistance ability.Because bio-feritlizer can manufacture and assist Crop to utilize various nutrient elements, therefore the quality of agricultural-food is had greatly improved, can change the present situation of " melon is not fragrant, and fruit is sweetless; tea is tasteless " because producing to fertilize, make agricultural-food indices reach the standard of green food.
Composite bio-fertilizer is effective combination of natural organic substance and biotechnology.The microbial inoculum that it comprises, has the organic substance of acceleration Decomposition, manufactures or transforms available nutrient " power " is provided for crop.Microbial inoculum is with the effect that improves potential nutrients in chemical fertilizer utilization ratio and activating soil simultaneously.
Bio-feritlizer kind is a lot, but most or traditional fixed nitrogen, phosphorus decomposing, potassium bacterium, have even also with yeast, lack new and effective product.Bacterial strain target usefulness is unstable, fill a prescription unreasonable, resistance is poor, so the research of bio-fertilizer is also existed to very large difficulty.
Summary of the invention
The present invention, for solving prior art problem, provides a kind of novel, environment friendly biological fertilizer.This bio-fertilizer, by the collaborative promoter action of multiple-microorganism, can significantly improve soil fertility, manufactures and assists Crop to utilize various nutrient elements, suppresses the generation of disease and pest, the standard that agricultural-food indices reaches green food that obtains.
Applicant finds by long-term screening study, between subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis, can produce synergy, effectively increases soil fertility and the resistance against diseases of crop, thereby facilitates the present invention.
One aspect of the present invention provides a kind of micro organism composite fertilizer, and straw powder, urea, Repone K and diatomite by microbiobacterial agent, after becoming thoroughly decomposed form.
Each component of mentioned microorganism composite fertilizer and mass ratio thereof are respectively: microbiobacterial agent 30-100 part, straw powder 100-300 part, urea 100-150 part, Repone K 50-120 part and diatomite 80-150 part after becoming thoroughly decomposed.
Mentioned microorganism microbial inoculum by subtilis (
bacillus subtilis), Pseudomonas aeruginosa (
pseudomonas aeruginosa), plant lactobacillus (
lactobacillus plantarum) and Bacillus thuringiensis (
bacillus thuringiensis) form.
The preferred subtilis CGMCC 1.3376 of component, Pseudomonas aeruginosa CGMCC1.860, plant lactobacillus CGMCC1.6971 and Bacillus thuringiensis CGMCC1.788 in mentioned microorganism preparation.
In mentioned microorganism microbial inoculum, the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram.
In mentioned microorganism composite fertilizer, the content of each component is preferably respectively: 110 parts, 80 parts of microbiobacterial agents, 90 parts, 140 parts, 210 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite.
One aspect of the present invention provides the application of mentioned microorganism composite fertilizer in proportion of crop planting.
The consumption of mentioned microorganism composite fertilizer is 10-20 gram/meter
2.
The present invention provides the production method of mentioned microorganism composite fertilizer on the other hand, comprises the steps:
1) subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis are adopted respectively to high density liquid fermentation, by zymotechnique post-processing technology, make gemma content up to 10
11-10
12the super dense microbiobacterial agent of CFU/g, wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram;
2) take in proportion microbiobacterial agent 30-100 part that step 1) makes, straw powder 100-300 part, urea 100-150 part, Repone K 50-120 part, diatomite 80-150 part after becoming thoroughly decomposed, even in stirrer for mixing;
3) with extruding type tablets press, carry out granulation;
4) by the good material of granulation air-dry micro organism composite fertilizer of the present invention in air-dry cylinder.
The preferred subtilis CGMCC 1.3376 of above-mentioned subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis, Pseudomonas aeruginosa CGMCC1.860, plant lactobacillus CGMCC1.6971 and Bacillus thuringiensis CGMCC1.788.
beneficial effect
Micro organism composite fertilizer of the present invention can significantly improve soil compaction problem, improve the utilization ratio of fertilizer, improve fertilizer efficiency, microorganism wherein generates various nutrient elements in reproductive process, not only improved crop yield, and the indices of the agricultural-food that obtain reaches the standard of green food; The interpolation of Bacillus thuringiensis can effectively suppress the generation of disease and pest in addition, reduces the use of agricultural chemicals, is conducive to environment protection and food safety.The present invention also provides the production method of mentioned microorganism composite fertilizer, and the method is simple to operate, and operation is few, and is applicable to existing shop equipment, is suitable for extensively and promotes.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that after having read content of the present invention, those skilled in the art can make various changes or modification to the present invention, and these equivalent form of values fall into the application's appended claims limited range equally.
The all ingredients the present invention relates to and equipment, all can be selected from commercially available any one; The present invention relates in bacterial strain subtilis bacterium purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.3376; Pseudomonas aeruginosa is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.860; Plant lactobacillus is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.6971; Bacillus thuringiensis is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.788.
embodiment 1
A kind of micro organism composite fertilizer, straw powder, urea, Repone K and diatomite by microbiobacterial agent, after becoming thoroughly decomposed form, and each component and mass ratio thereof are respectively: 80 parts, 30 parts of microbiobacterial agents, 50 parts, 100 parts, 100 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite.
Mentioned microorganism microbial inoculum is comprised of subtilis, Pseudomonas aeruginosa, plant lactobacillus and Bacillus thuringiensis, and wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram.
The production method of mentioned microorganism composite fertilizer, comprises the steps:
1) subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis are adopted respectively to high density liquid fermentation, by zymotechnique post-processing technology, make gemma content up to 10
11-10
12the super dense microbiobacterial agent of CFU/g, wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram;
2) take in proportion 30 parts of microbiobacterial agents that step 1) makes, 80 parts, 50 parts, 100 parts, 100 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite, even in stirrer for mixing;
3) with extruding type tablets press, carry out granulation;
4) by the good material of granulation air-dry micro organism composite fertilizer of the present invention in air-dry cylinder.
embodiment 2
A kind of micro organism composite fertilizer, straw powder, urea, Repone K and diatomite by microbiobacterial agent, after becoming thoroughly decomposed form, and each component and mass ratio thereof are respectively: 150 parts, 100 parts of microbiobacterial agents, 120 parts, 150 parts, 300 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite.
Mentioned microorganism microbial inoculum is comprised of subtilis, Pseudomonas aeruginosa, plant lactobacillus and Bacillus thuringiensis, and wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram.
The production method of mentioned microorganism composite fertilizer, comprises the steps:
1) subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis are adopted respectively to high density liquid fermentation, by zymotechnique post-processing technology, make gemma content up to 10
11-10
12the super dense microbiobacterial agent of CFU/g, wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram;
2) take in proportion 100 parts of microbiobacterial agents that step 1) makes, 150 parts, 120 parts, 150 parts, 300 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite, even in stirrer for mixing;
3) with extruding type tablets press, carry out granulation;
4) by the good material of granulation air-dry micro organism composite fertilizer of the present invention in air-dry cylinder.
embodiment 3
A kind of micro organism composite fertilizer, straw powder, urea, Repone K and diatomite by microbiobacterial agent, after becoming thoroughly decomposed form, and each component and mass ratio thereof are respectively: 110 parts, 80 parts of microbiobacterial agents, 90 parts, 140 parts, 210 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite.
Mentioned microorganism microbial inoculum is comprised of subtilis, Pseudomonas aeruginosa, plant lactobacillus and Bacillus thuringiensis, and wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram.
The production method of mentioned microorganism composite fertilizer, comprises the steps:
1) subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis are adopted respectively to high density liquid fermentation, by zymotechnique post-processing technology, make gemma content up to 10
11-10
12the super dense microbiobacterial agent of CFU/g, wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram;
2) take in proportion 80 parts of microbiobacterial agents that step 1) makes, 110 parts, 90 parts, 140 parts, 210 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite, even in stirrer for mixing;
3) with extruding type tablets press, carry out granulation;
4) by the good material of granulation air-dry micro organism composite fertilizer of the present invention in air-dry cylinder.
the application of embodiment 4 micro organism composite fertilizers in proportion of crop planting
In suburb, choose that soil property is fertile, the uniform plot of soil fertility, the Chinese cabbage seedling of planting, uses the micro organism composite fertilizer that embodiment of the present invention 1-3 produces, 10-20 gram of/meter of during respectively at 1 month, two months
2, as a control group, use the inorganic fertilizer of same fertilizer efficiency, in the time of 3 months, the Chinese cabbage of experimental group and control group to be tested and assessed, result is as shown in the table:
| Process | Plant height | Ball is high | Transverse diameter | Blade profile index |
| Control group | 35.2 | 23.2 | 15.5 | 1.7 |
| Test group | 37.9 | 24.1 | 16.2 | 1.8 |
From table data can find out, micro organism composite fertilizer of the present invention can effectively promote the growth of Chinese cabbage, thereby also illustrates that micro organism composite fertilizer of the present invention can significantly improve fertilizer efficiency, improves the fertility of soil, is with a wide range of applications.
Claims (5)
1. a method of producing bio-feritlizer, comprises the steps:
1) subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis are adopted respectively to high density liquid fermentation, by zymotechnique post-processing technology, make gemma content up to 10
11-10
12the super dense microbiobacterial agent of CFU/g, wherein the viable count of subtilis is 10
9-10
10individual/gram, the viable count of verdigris Zymomonas mobilis is 10
7-10
8individual/gram, the viable count of plant lactobacillus is 10
8-10
9individual/gram, the viable count of Bacillus thuringiensis is 10
7-10
8individual/gram;
2) take in proportion microbiobacterial agent 30-100 part that step 1) makes, straw powder 100-300 part, urea 100-150 part, Repone K 50-120 part, diatomite 80-150 part after becoming thoroughly decomposed, even in stirrer for mixing;
3) with extruding type tablets press, carry out granulation;
4) by the good material of granulation air-dry bio-feritlizer of the present invention in air-dry cylinder.
2. the method for claim 1, it is characterized in that, subtilis, verdigris Zymomonas mobilis, plant lactobacillus and Bacillus thuringiensis are subtilis CGMCC 1.3376, Pseudomonas aeruginosa CGMCC1.860, plant lactobacillus CGMCC1.6971 and Bacillus thuringiensis CGMCC1.788 described in step 1).
3. method as claimed in claim 1, it is characterized in that step 2) described microbiobacterial agent, straw powder, urea, Repone K and diatomaceous mass ratio after becoming thoroughly decomposed be respectively 80 parts of microbiobacterial agents, 110 parts, 90 parts, 140 parts, 210 parts of straw powder, urea, Repone K after becoming thoroughly decomposed and diatomite.
4. described in above-mentioned arbitrary claim, method is produced the bio-feritlizer obtaining.
5. the application of bio-feritlizer in proportion of crop planting described in claim 4.
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| CN201310489855.0A CN103539548A (en) | 2013-10-18 | 2013-10-18 | Method for producing bio-fertilizer |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101417897A (en) * | 2007-10-25 | 2009-04-29 | 李智法 | Microorganism organic composite fertilizer |
| CN102267838A (en) * | 2010-08-27 | 2011-12-07 | 苏州仁成生物科技有限公司 | Microbial composite fertilizer and preparation method thereof |
| CN102267842A (en) * | 2011-06-24 | 2011-12-07 | 东北师范大学 | Organic-inorganic block controlled release fertilizer and preparation method thereof |
| CN102408274A (en) * | 2010-09-25 | 2012-04-11 | 北京世纪阿姆斯生物技术有限公司 | Biological fertilizer and method for producing biological fertilizer |
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2013
- 2013-10-18 CN CN201310489855.0A patent/CN103539548A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101417897A (en) * | 2007-10-25 | 2009-04-29 | 李智法 | Microorganism organic composite fertilizer |
| CN102267838A (en) * | 2010-08-27 | 2011-12-07 | 苏州仁成生物科技有限公司 | Microbial composite fertilizer and preparation method thereof |
| CN102408274A (en) * | 2010-09-25 | 2012-04-11 | 北京世纪阿姆斯生物技术有限公司 | Biological fertilizer and method for producing biological fertilizer |
| CN102267842A (en) * | 2011-06-24 | 2011-12-07 | 东北师范大学 | Organic-inorganic block controlled release fertilizer and preparation method thereof |
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Application publication date: 20140129 |