CN103504122A - Feed fermentation method with modified attapulgite clay as nitrogen fixing agent - Google Patents
Feed fermentation method with modified attapulgite clay as nitrogen fixing agent Download PDFInfo
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- CN103504122A CN103504122A CN201310447583.8A CN201310447583A CN103504122A CN 103504122 A CN103504122 A CN 103504122A CN 201310447583 A CN201310447583 A CN 201310447583A CN 103504122 A CN103504122 A CN 103504122A
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- attapulgite clay
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Abstract
The invention discloses a feed fermentation method with modified attapulgite clay as a nitrogen fixing agent. High-temperature ammoniation straws used as feed fermentation substrates are fermented at high temperature with the modified attapulgite clay as the nitrogen fixing agent by adopting a mixed fermentation bacterium, wherein the mixed fermentation bacterium comprises the mixed fermentation bacterium I and the mixed fermentation bacterium II. The fermentation steps are as follows: the modified attapulgite clay is prepared; the nitrogen fixing agent is added in the straws for high-temperature ammoniation to obtain the fermentation substrates; the modified attapulgite clay is added in the fermentation substrates; the mixed fermentation bacteria I and II are added in the fermentation substrates in batches for fermentation; finally, the fermentation materials are dried or pelletized to obtain straw fermented feed. The method has the following advantages: the modified attapulgite clay is adopted as the nitrogen fixing agent to be applied to the feed fermentation process, so that a better nitrogen preservation effect can be achieved, the optimal effect of fermenting the high-temperature ammoniation straws is obtained, and the nitrogen content of the ammoniation straw fermentation feed can be effectively improved.
Description
Technical field
The present invention relates to improve the method that straw nutrition is worth, particularly a kind ofly using the feed fermentation method of modified attapulgite clay as nitrogen fixation agent.
Background technology
Stalk is directly lower as feed nutritive value, and digestibility only has 25-40%, and its palatability is poor, and feed intake is low, only has the 50-60% of high quality forage, is difficult to reach the needs that maintain of animal.But stalk is still the feed resource with great potential, once after chemistry or biological method processing, its nutritive value is improved, and just can be used as the main roughage of ruminant.For a long time, nutritionist is devoted to the quality problems how research improves Low quality roughage always, and searching can improve the processing method of stalk digestibility and nutritive value, and has made huge effort for this reason.
Ammoniated straw is to improve straw nutrition to be worth more successful method.Ammonia is alkalescent, but the ability of its swelling fiber is suitable with NaOH, and main is that ammonifying capacity significantly improves the crude protein content of stalk, for rumen microorganism under low protein level provides more nitrogenous source.Relevant Electronic Speculum result also shows, the ammonification of roughage is processed and can be made its institutional framework change, more easily for rumen microorganism adheres to and utilize.But the problem that ammonia volatilization easily appears in feed is prepared in ammoniated straw fermentation, causes nitrogen loss.And the crystal structure of Concave-convex clay rod uniqueness has been given its special performance, as large specific area and adsorptivity, cation interchangeability etc.Some research explanations in past, under suitable condition, Concave-convex clay rod has certain suction-operated to ammonia nitrogen, is therefore also widely used in the aspects such as food industry, building industry or purifying water body, but not yet application to some extent on microorganism fermentation field.
This patent, in conjunction with these characteristics of Concave-convex clay rod, proposes modified attapulgite clay to be applied in feed fermentation field, can effectively improve the nitrogen content of ammoniated straw fermented feed.
Summary of the invention
The object of the invention is for above-mentioned existing problems, a kind of feed fermentation method of modified attapulgite clay as nitrogen fixation agent of usining is provided, adopt this feed fermentation method can obtain good guarantor's nitrogen effect, thereby high temperature ammoniated straw is fermented and produces optimum efficiency, can effectively improve the nitrogen content of ammoniated straw fermented feed.
Technical scheme of the present invention:
A kind ofly using the feed fermentation method of modified attapulgite clay as nitrogen fixation agent, using high temperature ammoniated straw as feed fermentation substrate, using modified attapulgite clay as nitrogen fixation agent, adopt mixed culture fermentation bacterium that high temperature ammoniated straw is at high temperature fermented, mixed culture fermentation bacterium comprises mixed culture fermentation bacterium No. I and mixed culture fermentation bacterium No. II, and fermentation step is as follows:
1) Concave-convex clay rod is pulverized to rear 100 mesh sieves of crossing, then the Concave-convex clay rod crushing is added in sulfuric acid solution, under room temperature, stir 3h, with distilled water, being washed to pH value is 5.8-6.2, after centrifugal, solid formation is dried or natural air drying at 60 ℃, make modified attapulgite clay standby;
2) nitrogen fixation agent is added in stalk, under 85-95 ℃ of temperature conditions, carry out aminating reaction 4-8h, make high temperature ammoniated straw, the HCl solution that is then 1mol/L by concentration is adjusted to 5.8-6.2 by ammoniated straw pH, add distilled water that moisture content is adjusted to 65-75%, standby as fermentation substrate;
3) nitrogen fixation agent modified attapulgite clay is added in fermentation substrate and mixed;
4) mixed culture fermentation bacterium is joined in batches in the fermentation substrate that is added with nitrogen fixation agent for No. II and fermented with mixed culture fermentation bacterium for No. I, fermentation temperature is 25-30 ℃, fermentation time is 5-7 days, every day, turning was 1-2 time, then in being added with the fermentation substrate of nitrogen fixation agent, add No. I fermentation of mixed culture fermentation bacterium after 5-7 days, mixed culture fermentation bacterium is added for No. II in fermentation material and proceeds to ferment, fermentation temperature is 28-32 ℃, fermentation time is 4-6 days, every day, turning was 1-2 time, described mixed culture fermentation bacterium No. I is mould by healthy and free from worry wood, aspergillus niger and mushroom in mass ratio 1:1:1 mix composition, mixed culture fermentation bacterium No. II is mixed and forms by 1:1 in mass ratio by saccharomyces cerevisiae and candida utili, five strain bacterium used are all purchased from Chinese common micro-organisms culture presevation administrative center,
5), after sweat finishes, by fermentation materials drying or granulation, can make straw fermented feed.
In described step 1), the concentration of sulfuric acid solution is 2mol/L, and the volume ratio of Concave-convex clay rod and sulfuric acid solution is 1:10.
Described step 2) nitrogen fixation agent in is ammoniacal liquor or urea liquid, wherein the concentration of ammoniacal liquor is 25wt%, the addition of ammoniacal liquor is counted the 2-4% of stalk dry biomass by ammonia quality, the concentration of urea liquid is 15wt%, and the addition of urea liquid is counted the 3-5% of stalk dry biomass by the quality of urea.
In described step 3), the addition of modified attapulgite clay is the 1-3% of fermentation substrate dry biomass.
In described step 4), the addition of mixed culture fermentation bacterium No. I is the 4-6% of fermentation substrate dry biomass, the 5-8% of the addition fermentation substrate dry biomass that mixed culture fermentation bacterium is No. II.
Advantage of the present invention is: using modified attapulgite clay as nitrogen fixation agent, be applied in feed fermentation process, can obtain good guarantor's nitrogen effect, thereby high temperature ammoniated straw is fermented and produces optimum efficiency, can effectively improve the nitrogen content of ammoniated straw fermented feed.
The specific embodiment
Embodiment:
In this embodiment, mixed culture fermentation bacterium relates to for No. I healthy and free from worry wood mould [
trichoderma koningii, 1579 (=G79)], aspergillus niger (
aspergillus niger, ATCC 16888), mushroom (
lentinus edodes, LN050), the saccharomyces cerevisiae that mixed culture fermentation bacterium relates to for No. II (
saccharomyces cerevisiae, A22), candida utili (
candida utilis, Y34), be all purchased from Chinese common micro-organisms culture presevation administrative center.
A kind ofly using the feed fermentation method of modified attapulgite clay as nitrogen fixation agent, using high temperature ammoniated straw as feed fermentation substrate, using modified attapulgite clay as nitrogen fixation agent, adopt mixed culture fermentation bacterium that high temperature ammoniated straw is at high temperature fermented, mixed culture fermentation bacterium comprises mixed culture fermentation bacterium No. I and mixed culture fermentation bacterium No. II, and fermentation step is as follows:
1) Concave-convex clay rod is pulverized to rear 100 mesh sieves of crossing, then the Concave-convex clay rod crushing being added to concentration is in the sulfuric acid solution of 2mol/L, the volume ratio of Concave-convex clay rod and sulfuric acid solution is 1:10, under room temperature, stir 3h, with distilled water, being washed to pH value is 6, after centrifugal, solid formation is dried at 60 ℃, make modified attapulgite clay standby;
2) ammoniacal liquor that is 25wt% by concentration adds in stalk, under 90 ℃ of temperature conditions, carry out aminating reaction 4h, make high temperature ammoniated straw, the addition of ammoniacal liquor is counted 3% of stalk dry biomass by ammonia quality, then the HCl solution that is 1mol/L by concentration is adjusted to 6 by ammoniated straw pH value, add distilled water that moisture content is adjusted to 65-75%, standby as fermentation substrate;
3) nitrogen fixation agent modified attapulgite clay is added in fermentation substrate and mixed, the quality that adds of modified attapulgite clay is 2% of fermentation substrate dry biomass;
4) mixed culture fermentation bacterium is joined in batches in the fermentation substrate that is added with nitrogen fixation agent for No. II and fermented with mixed culture fermentation bacterium for No. I, the interpolation quality that mixed culture fermentation bacterium is No. I is 6% of fermentation substrate dry biomass, fermentation temperature is 25-30 ℃, fermentation time is 6 days, every day, turning was 1-2 time, in being added with the fermentation substrate of nitrogen fixation agent, add No. I fermentation of mixed culture fermentation bacterium after 6 days, mixed culture fermentation bacterium is added for No. II in fermentation material and proceeds to ferment, the interpolation quality that mixed culture fermentation bacterium is No. II is 7% of fermentation substrate dry biomass, fermentation temperature is 28-32 ℃, fermentation time is 5 days, every day, turning was 1-2 time, described mixed culture fermentation bacterium No. I by healthy and free from worry wood mould [
richoderma koningii, 1579 (=G79)], aspergillus niger (
aspergillus niger, ATCC 16888) and mushroom (
lentinus edodes, LN050) 1:1:1 mix to form in mass ratio, mixed culture fermentation bacterium No. II by saccharomyces cerevisiae (
saccharomyces cerevisiae, A22) and candida utili (
candida utilis, Y34) press 1:1 in mass ratio and mix composition,
5), after sweat finishes, after fermentation materials is dried, can make straw fermented feed.
Using the feed fermentation experiment of modified attapulgite clay as nitrogen fixation agent: detect modified attapulgite clay in stalk fermentation substrate different interpolation mass percent on fermented feed in the impact of crude protein percentage composition, experimental result is as shown in the table:
| Modified attapulgite adds percentage (wt%) | 0 | 1 | 1.5 | 2 | 2.5 | 3 |
| Fermented feed crude protein content (wt%) | 12.92 | 16.16 | 18.74 | 19.26 | 19.15 | 19.18 |
Experimental result shows: the modified attapulgite clay of usining makes an addition in straw feed fermentation as nitrogen fixation agent, can effectively improve the nitrogen content of ammoniated straw fermented feed.
Claims (5)
1. using the feed fermentation method of modified attapulgite clay as nitrogen fixation agent for one kind, it is characterized in that: using high temperature ammoniated straw as feed fermentation substrate, using modified attapulgite clay as nitrogen fixation agent, adopt mixed culture fermentation bacterium that high temperature ammoniated straw is at high temperature fermented, mixed culture fermentation bacterium comprises mixed culture fermentation bacterium No. I and mixed culture fermentation bacterium No. II, and fermentation step is as follows:
1) Concave-convex clay rod is pulverized to rear 100 mesh sieves of crossing, then the Concave-convex clay rod crushing is added in sulfuric acid solution, under room temperature, stir 3h, with distilled water, being washed to pH value is 5.8-6.2, after centrifugal, solid formation is dried or natural air drying at 60 ℃, make modified attapulgite clay standby;
2) nitrogen fixation agent is added in stalk, under 85-95 ℃ of temperature conditions, carry out aminating reaction 4-8h, make high temperature ammoniated straw, the HCl solution that is then 1mol/L by concentration is adjusted to 5.8-6.2 by ammoniated straw pH, add distilled water that moisture content is adjusted to 75%, standby as fermentation substrate;
3) nitrogen fixation agent modified attapulgite clay is added in fermentation substrate and mixed;
4) mixed culture fermentation bacterium is joined in batches in the fermentation substrate that is added with nitrogen fixation agent for No. II and fermented with mixed culture fermentation bacterium for No. I, fermentation temperature is 25-30 ℃, fermentation time is 5-7 days, every day, turning was 1-2 time, then in being added with the fermentation substrate of nitrogen fixation agent, add No. I fermentation of mixed culture fermentation bacterium after 5-7 days, mixed culture fermentation bacterium is added for No. II in fermentation material and proceeds to ferment, fermentation temperature is 28-32 ℃, fermentation time is 4-6 days, every day, turning was 1-2 time, described mixed culture fermentation bacterium No. I is mould by healthy and free from worry wood, aspergillus niger and mushroom in mass ratio 1:1:1 mix composition, mixed culture fermentation bacterium No. II is mixed and forms by 1:1 in mass ratio by saccharomyces cerevisiae and candida utili, five strain bacterium used are all purchased from Chinese common micro-organisms culture presevation administrative center,
5), after sweat finishes, by fermentation materials drying or granulation, can make straw fermented feed.
2. using according to claim 1 modified attapulgite clay as the feed fermentation method of nitrogen fixation agent, it is characterized in that: in described step 1), the concentration of sulfuric acid solution is 2mol/L, the volume ratio of Concave-convex clay rod and sulfuric acid solution is 1:10.
3. using according to claim 1 the feed fermentation method of modified attapulgite clay as nitrogen fixation agent, it is characterized in that: the nitrogen fixation agent described step 2) is ammoniacal liquor or urea liquid, wherein the concentration of ammoniacal liquor is 25wt%, the addition of ammoniacal liquor is counted the 2-4% of stalk dry biomass by ammonia quality, the concentration of urea liquid is 15wt%, and the addition of urea liquid is counted the 3-5% of stalk dry biomass by the quality of urea.
4. using according to claim 1 modified attapulgite clay as the feed fermentation method of nitrogen fixation agent, it is characterized in that: in described step 3), the addition of modified attapulgite clay is the 1-3% of fermentation substrate dry biomass.
5. using according to claim 1 the feed fermentation method of modified attapulgite clay as nitrogen fixation agent, it is characterized in that: in described step 4), the addition of mixed culture fermentation bacterium No. I is the 4-6% of fermentation substrate dry biomass, the 5-8% of the addition fermentation substrate dry biomass that mixed culture fermentation bacterium is No. II.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108740440A (en) * | 2018-06-25 | 2018-11-06 | 宿松县华图生物科技有限公司 | Pig's feed and preparation method thereof that is a kind of full of nutrition and improving stomach |
| CN115259916A (en) * | 2022-09-14 | 2022-11-01 | 甘肃省农业科学院土壤肥料与节水农业研究所 | Carbon-nitrogen combined fixing agent, preparation method thereof and application thereof in preparation of organic fertilizer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1476771A (en) * | 2002-08-20 | 2004-02-25 | 甘肃凯西生态环境工程有限公司 | Slowly-released non-protein nitrogen |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1476771A (en) * | 2002-08-20 | 2004-02-25 | 甘肃凯西生态环境工程有限公司 | Slowly-released non-protein nitrogen |
Non-Patent Citations (2)
| Title |
|---|
| 刘乐等: "纤维素降解细菌筛选及降解特性分析", 《环境污染与防治》 * |
| 刘金鹏等: "中国农业秸秆资源化技术及产业发展分析", 《生态经济》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108740440A (en) * | 2018-06-25 | 2018-11-06 | 宿松县华图生物科技有限公司 | Pig's feed and preparation method thereof that is a kind of full of nutrition and improving stomach |
| CN115259916A (en) * | 2022-09-14 | 2022-11-01 | 甘肃省农业科学院土壤肥料与节水农业研究所 | Carbon-nitrogen combined fixing agent, preparation method thereof and application thereof in preparation of organic fertilizer |
| CN115259916B (en) * | 2022-09-14 | 2023-07-21 | 甘肃省农业科学院土壤肥料与节水农业研究所 | Carbon-nitrogen combined fixing agent, preparation method thereof and application thereof in preparation of organic fertilizer |
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