CN103494326A - Biological enzyme mixture impurity-removing and tobacco-alcoholization method - Google Patents
Biological enzyme mixture impurity-removing and tobacco-alcoholization method Download PDFInfo
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- CN103494326A CN103494326A CN201310491694.9A CN201310491694A CN103494326A CN 103494326 A CN103494326 A CN 103494326A CN 201310491694 A CN201310491694 A CN 201310491694A CN 103494326 A CN103494326 A CN 103494326A
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Abstract
The invention discloses a biological enzyme mixture impurity-removing and tobacco-alcoholization method. The method comprises the steps that a biological enzyme mixture solution formed by pectinase compounds, vegetable protein proteolytic enzymes, native starch degrading enzymes, lipases and water is sprayed on flue-cured tobaccos which are poor in color, aroma and taste during the baking process or after the baking process, then the tobaccos are baked in an adobe curing barn or a heat-exchange dense curing barn at the temperature from 30 DEG C to 55 DEG C and in the humidity of 30 %; the alcoholization process for the tobaccos are baked for 7 to 8 days, the alcoholization process of the flue-cured tobaccos which are poor in color, aroma and taste is accelerated at 6 p.m. to 12 p.m. in a day, the cleanliness of fresh tobaccos is improved, and the flue-cured tobaccos which are poor in quality recover to have the original good or better color, aroma and taste. The biological enzyme mixture impurity-removing and tobacco-alcohorization method is suitable for the fresh tobaccos or low-graded flue-cured tobaccos, and is also suitable for lamina which is hit and baked again or cut tobaccos. The method can reduce tar, reduce the deposition of PM 2.5 particles in the tar in human body pulmonary alveoli, reduces the harm to human body health of smoking, enables the tobaccos to recover to have the original or better color, aroma and taste and enables the number of the low-graded tobaccos to be greatly reduced, and tobacco growers, tobacco dealers and tobacco enterprises can obtain better economical benefits.
Description
Technical field
The present invention relates to a kind of biology enzyme mixture aqueous solution that utilizes and remove objectionable impurities in tobacco, accelerate the method for alcoholized tobacco simultaneously, belong to technical field of tobacco processing.
Background technology
The security of tobacco leaf directly affects the safety of tobacco product, and the impurity of removing tobacco leaf is important, as: flue-cured tobacco surface chloasma, blackspot, mildew etc. belong to organic substance residues impurity, can have a strong impact on the color and luster of flue-cured tobacco; Dust, heavy metal etc. belong to inorganic matter impurity.Above impurity should be removed as far as possible, guarantees the cleanliness factor of flue-cured tobacco.After flue-cured tobacco rolls into cigarette, the pm2.5 amounts of particles in its tar content, particularly tar can reduce greatly.Reduce pm2.5 particle in tar and, in the IA deposition of human body, alleviate the injury of smoking to health.Virginian-type cigarette is the major product in China's cigarette industry, and the quantity of tobacco planting and baking is huge.New fresh tobacco leaf before baking or flue-cured tobacco use the biology enzyme mixture aqueous solution can improve the flue-cured tobacco cleanliness factor, remove impurity, the recovery flue-cured tobacco should have or better color, and discarded tobacco leaf quantity can greatly reduce, and tobacco grower, tobacco leaf are sold with cigarette enterprise will therefrom obtain huge economic benefit.
Summary of the invention
The purpose of this invention is to provide the removal of impurities of a kind of biology enzyme mixture and refine the method for tobacco, in order to improve the quality of flue-cured tobacco, reduce the injury of smoking to human body.
The technical solution used in the present invention is: in fresh Tobacco Leaf Curing, or after baking on the cured tobacco leaf of color inequality, the biology enzyme mixture aqueous solution that sprinkling is comprised of pectase compound, vegetable protein hydrolase, ative starch digestive enzyme and lipase and water, then in sun-dried mud brick barn or heat exchange bulk curing barn, toast, temperature 30-55 ℃, humidity 30%; Time: new fresh tobacco leaf is 7-8 days; Under the color condition that all poor flue-cured tobacco is 18-24 hour, accelerate the alcoholized tobacco process, improve the cleanliness factor of flue-cured tobacco, and make the flue-cured tobacco of inferior quality recover originally should have or better color, its concrete grammar comprises the steps:
One, the preparation of biology enzyme mixture:
1.1.1 the preparation of pectase compound: 1 part of protopectinase, 1 part of pectin formicester hydrolase, 1 part of polygalacturonase, 1 part of pectate lyase, obtain the pectase compound after fully mixing, standby;
1.1.2 the biochemical reaction principle of pectase compound:
Pectic substance is under the protopectinase effect, and water soluble pectin is under the effect of pectin formicester hydrolase, and pectic acid, under the acting in conjunction of polygalacturonase and pectate lyase, generates galacturonic acid, makes the pectic substance Partial digestion between tobacco cell;
1.1.3 pectase compound ph value 2.5-6.0 temperature characterisitic: 35-55 ℃,
Reaction time: 10-24 hour, shared component 0.5-1 part;
1.2.1 it is aromatic amino acid or the acid amino peptide bond formed that the vegetable protein hydrolase acts on the main position of tobacco protein, a part is hydrolyzed into peptone, and another part is broken down into the amino acid that comprises tyrosine, phenylalanine, glutamine, proline;
1.2.2 vegetable protein hydrolase ph value 1.0-5.0 temperature characterisitic 30-50 ℃, reaction time: 10-24 hour shared component 0.5-1 part;
1.3.1 the ative starch digestive enzyme, organic impurities and the inorganic matter impurity of degraded tobacco leaf surface,
Ph value: 4.0-4.5, temperature characterisitic 35-55 ℃, reaction time: 20-24 hour, shared component 5-6 part;
1.4.1 lipase, this enzymatic fat splitting is glycerine and aliphatic acid, add this enzyme in tobacco, can make the tobacco section fat acid decomposition become aliphatic or aromatic carbonyls, dicarbonyl compound cigarette Studies of The Aromatic Substances, therefore can increase and improve local flavor and the fragrance of tobacco;
1.4.2 in tobacco, part fat is degraded to the cigarette Studies of The Aromatic Substances, in tobacco, fats portion reduces; Tobacco tar content when burning and sucking can descend;
1.4.3 in tobacco, part fat is fallen when changing, chromolipid also part discharges, and improves the color and luster of flue-cured tobacco;
1.4.4 temperature characterisitic 30-50 ℃ of lipase ph value 4.0-9.0 (best ph value 7.0) (omnidistance temperature is no more than 55 ℃), reaction time 12-24 hour shared component 3-4 part;
1.4.5 the impact of metal ion on lipase: activated by calcium, magnesium ion, resisted by copper, mercury, lead ion;
1.4.6 lipase is the yellowish-brown powder, water-soluble, while using this enzyme or this multienzyme complex aqueous solution, health does not directly contact with this enzyme and this multienzyme complex aqueous solution, during operation, uses glue to mould gloves, if there is contact to rinse with clear water in time;
1.5.1 by evenly mixing after the different component preparations of above-mentioned composition, add suitable quantity of water, total amount is 100 parts, obtains biology enzyme mixture aqueous solution final products again.
Two, the use of biology enzyme mixture:
2.1.1 biology enzyme mixture aqueous solution using method, comprise the steps:
(1) the biology enzyme mixture aqueous solution sprays or soaks on new fresh tobacco leaf or all poor flue-cured tobaccos of color;
(2) tobacco leaf of step 1 is put into to sun-dried mud brick barn or heat exchange bulk curing barn, temperature is 30-55 ℃; Humidity is 30%; Time is: new fresh tobacco leaf 7-8 days; The all poor flue-cured tobacco 18-24 hour of color;
(3) tobacco leaf or flue-cured tobacco after baking are taken out from barn, naturally cooling after, obtain removal of impurities and refine after the tobacco finished product;
2.1.2 the biology enzyme mixture aqueous solution product of making by this method is applicable to new fresh tobacco leaf or sodium hydroxide, also is applicable to the sheet cigarette after beating and double roasting, or pipe tobacco.
Compared with prior art, the present invention has following characteristics and progress:
(1) social benefit: reduce tar, reduce PM2.5 particle in tar and, in the IA deposition of human body, alleviate the injury of smoking to health;
(2) economic benefit: the recovery flue-cured tobacco should have or better color, and discarded tobacco leaf quantity can greatly reduce; Tobacco grower, tobacco leaf are sold with cigarette enterprise will obtain better income.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described:
Example 1: to the method for removal of impurities and the alcoholization of new fresh tobacco leaf, it comprises the steps:
New fresh tobacco leaf 100kg is sprayed to the biology enzyme mixture aqueous solution, this aqueous solution is pressed 0.5 part of pectase compound, 1 part of vegetable protein hydrolase, 5 parts of ative starch digestive enzymes, 2 parts, lipase, 91.5 parts, water, make through mix and blend, then, after the new fresh tobacco leaf soaked is suspended to bridge, send into heat exchange bulk curing barn or sun-dried mud brick barn, be 30% time baking 7 days at 30-55 ℃, humidity, obtain all good flue-cured tobaccos of color.
Example 2: will just be chosen as the flue-cured tobacco 100kg of B4F level, and spray the biology enzyme mixture aqueous solution, this biology enzyme mixture aqueous solution, press 1 part of pectase compound, 1 part of vegetable protein hydrolase, 6 parts of ative starch digestive enzymes, 4 parts, lipase, 88 parts, water, mix by fully stirring and make; After cured tobacco leaf after sprinkling is soaked is suspended to bridge, sending into heat exchange bulk curing barn or sun-dried mud brick barn, is 30% time at 30-55 ℃, humidity, and baking 18-24 hour, obtain the B2F flue-cured tobacco that quality promotes 2 grades.
Claims (2)
1. biology enzyme mixture removal of impurities refine the method for tobacco, it is characterized in that: in fresh Tobacco Leaf Curing or after baking on the cured tobacco leaf of color inequality, the biology enzyme mixture aqueous solution that sprinkling is comprised of pectase compound, vegetable protein hydrolase, ative starch digestive enzyme and lipase and water, then in sun-dried mud brick barn or heat exchange bulk curing barn, toast, temperature 30-55 ℃, humidity 30%; Time: new fresh tobacco leaf is 7-8 days; Under the color condition that all poor flue-cured tobacco is 18-24 hour, accelerate the alcoholized tobacco process, improve the cleanliness factor of flue-cured tobacco, and make the flue-cured tobacco of inferior quality recover originally should have or better color.
2. biology enzyme mixture as claimed in claim 1 removal of impurities refine the method for tobacco, it is characterized in that: its method comprises the steps:
One, the preparation of biology enzyme mixture:
1.1 the preparation of pectase compound: 1 part of protopectinase, 1 part of pectin formicester hydrolase, 1 part of polygalacturonase, 1 part of pectate lyase, obtain the pectase compound after fully mixing, standby;
1.2 the biochemical reaction principle of pectase compound:
Pectic substance is under the protopectinase effect, and water soluble pectin is under the effect of pectin formicester hydrolase, and pectic acid, under the acting in conjunction of polygalacturonase and pectate lyase, generates galacturonic acid, makes the pectic substance Partial digestion between tobacco cell;
1.3 pectase compound ph value 2.5-6.0 temperature characterisitic: 35-55 ℃,
Reaction time: 10-24 hour, shared component 0.5-1 part;
2.1 it is aromatic amino acid or the acid amino peptide bond formed that the vegetable protein hydrolase acts on the main position of tobacco protein, a part is hydrolyzed into peptone, and another part is broken down into the amino acid that comprises tyrosine, phenylalanine, glutamine, proline;
2.2 vegetable protein hydrolase ph value 1.0-5.0, temperature characterisitic 30-50 ℃, the reaction time:
10-24 hour, shared component 0.5-1 part;
3.1 the ative starch digestive enzyme, organic impurities and the inorganic matter impurity of degraded tobacco leaf surface,
Ph value: 4.0-4.5, temperature characterisitic 35-55 ℃, reaction time: 20-24 hour, shared component 5-6 part;
4.1 lipase, this enzymatic fat splitting is glycerine and aliphatic acid, add this enzyme in tobacco, can make the tobacco section fat acid decomposition become aliphatic or aromatic carbonyls, dicarbonyl compound cigarette Studies of The Aromatic Substances, therefore can increase and improve local flavor and the fragrance of tobacco;
4.2 in tobacco, part fat is degraded to the cigarette Studies of The Aromatic Substances, in tobacco, fats portion reduces; Tobacco tar content when burning and sucking can descend;
4.3 in tobacco, part fat is fallen when changing, chromolipid also part discharges, and improves the color and luster of flue-cured tobacco;
4.4 lipase ph value 4.0-9.0, temperature characterisitic 30-50 ℃, reaction time 12-24 hour, shared component 3-4 part;
4.5 the impact of metal ion on lipase: activated by calcium, magnesium ion, resisted by copper, mercury, lead ion;
4.6 lipase is the yellowish-brown powder, water-soluble, while using this enzyme or this multienzyme complex aqueous solution, health does not directly contact with this enzyme and this multienzyme complex aqueous solution, during operation, uses glue to mould gloves, if there is contact to rinse with clear water in time;
5.1 by evenly mixing after the different component preparations of above-mentioned composition, add suitable quantity of water, total amount is 100 parts, obtains biology enzyme mixture aqueous solution final products again;
Two, the use of biology enzyme mixture:
(1) the biology enzyme mixture aqueous solution made is sprayed or soaks on new fresh tobacco leaf or all poor flue-cured tobaccos of color;
(2) the new fresh tobacco leaf that will spray or soak through the biology enzyme mixture aqueous solution or all poor flue-cured tobaccos of color, put into sun-dried mud brick barn or heat exchange bulk curing barn, in temperature, is 30-55 ℃; Humidity is to toast under 30% condition, and baking time is: new fresh tobacco leaf 7-8 days; The all poor flue-cured tobacco 18-24 hour of color;
(3) tobacco leaf or flue-cured tobacco after baking are taken out from barn, naturally cooling after, obtain removal of impurities and refine after the tobacco finished product.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104886763A (en) * | 2015-06-25 | 2015-09-09 | 湖北中烟工业有限责任公司 | Application of amylase in cigar fermentation and quality improvement |
| CN104886762A (en) * | 2015-05-22 | 2015-09-09 | 云南中烟工业有限责任公司 | Alcoholization assistant agent capable of improving quality of tobacco leaves and application method thereof |
| CN105286073A (en) * | 2015-11-19 | 2016-02-03 | 云南省烟草公司昭通市公司 | Tobacco leaf curing modulating agent and using method thereof |
| CN106399277A (en) * | 2016-08-30 | 2017-02-15 | 康赞（北京）生物科技有限公司 | Enzyme preparation for tobacco processing and application of enzyme preparation |
| CN109924532A (en) * | 2019-04-19 | 2019-06-25 | 云南省烟草农业科学研究院 | A kind of removal flue-cured tobacco wax coat improves the method and its application of tobacco leaf dehydration characteristic |
| CN110214970A (en) * | 2019-05-30 | 2019-09-10 | 红云红河烟草(集团)有限责任公司 | Tobacco leaf enzyme treatment process and system |
| CN111480894A (en) * | 2020-03-25 | 2020-08-04 | 内蒙古昆明卷烟有限责任公司 | Cigarette cartridge containing biological enzyme and tobacco product |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104886762A (en) * | 2015-05-22 | 2015-09-09 | 云南中烟工业有限责任公司 | Alcoholization assistant agent capable of improving quality of tobacco leaves and application method thereof |
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| CN109924532B (en) * | 2019-04-19 | 2021-12-21 | 云南省烟草农业科学研究院 | Method for removing wax layer of flue-cured tobacco and improving water loss characteristic of tobacco leaf and application thereof |
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| CN111480894A (en) * | 2020-03-25 | 2020-08-04 | 内蒙古昆明卷烟有限责任公司 | Cigarette cartridge containing biological enzyme and tobacco product |
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Application publication date: 20140108 |