CN103463317A - Fish white head-white mouth disease treatment drug composition and preparation method thereof - Google Patents
Fish white head-white mouth disease treatment drug composition and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses a fish white head-white mouth disease treatment drug composition, which contains the following traditional Chinese medicines, by weight: 10-20 parts of alternanthera philoxeroides (Mart.) griseb., 20-30 parts of humifuse euphorbia herb, 2-10 parts of melia azedarach leaf, 15-25 parts of heartleaf houttuynia herb, 2-10 parts of herba cayatiae japonicae fresh grass, and 10-20 parts of rheum. According to the fish white head-white mouth disease treatment drug composition, humifuse euphorbia herb, melia azedarach leaf and heartleaf houttuynia herb have effects of antibacterial and bacterial inhibition, such that the components can be adopted as effective components so as to well prevent and treat white head-white mouth diseases, wherein the treatment efficiency is up to 99%. According to the preparation method, alternanthera philoxeroides (Mart.) griseb. is extracted by using a supercritical CO2 extraction method, such that activities of effective components of the alternanthera philoxeroides (Mart.) griseb. are retained, and the effective component content is high. In addition, the preparation method is simple and easy to perform, and is suitable for mass production.
Description
Technical field
The present invention relates to treat the field of Chinese medicines of fish disease, be specifically related to a kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease and preparation method thereof.
Background technology
The bacterial diseases of fishes that white head and mouth fish disease is caused by myxococcus (Myxococcus).Main harm summer flower fingerling.Sick fish is held to eyeball and is creamy white from kiss, lip swelling, close malfunctioning, dyspnea, mouthful surrounding skin is rotten to the corn becomes cotton-shaped.During morbidity, the necrocytosis around the forehead of sick fish and mouth, pigment disappears and performance white, and diseased region festers, and sometimes with canescence floss hair, thereby presents " the white mouth of hoary hair " symptom.The sick fish moved about at the water surface, symptom is particularly evident.After sick fish is dried up, symptom is just not remarkable.Serious sick fish, lesions position festers, and congested phenomenon appears in indivedual sick fish head section, sometimes also shows the pathological changes such as Rhizoma Euonymus, white smear, rotten tail, gill rot or the many mucus of whole body and reacts.The general body of sick fish is thin, blackout, accelerated breathing, and inappetence, swimming slowly, constantly emerges, dead soon.This disease is a kind of fulminant disease, and morbidity is exceedingly fast, and infects rapidly, can be all dead between one day.This sick epidemic season is apparent in view, generally in late May to early July, be onset peak June.
Alternanthera philoxeroides is included into " Chinese pharmacopoeia, this grass bitter in the mouth is cold in nature, nontoxic, and the function of heat clearing away, removing heat from blood, removing toxic substances is arranged, and cures mainly the diseases such as mass formed by blood stasis, venom, stranguria with turbid discharge, furuncle, eczema as a kind of Chinese medicine already.Alternanthera philoxeroides has significant antibacterial action to Gram-positive and negative bacteria, can obviously suppress the breeding of meningococcus, bacillus pyocyaneus, staphylococcus aureus, diphtheria corynebacterium, lung diplococcoid, viridans streptococci, bacterium flexneri, yeast bacillus etc.
The bright juice of Herba Euphorbiae Humifusae, decoct and water boiling concentration ethanol extract have obvious bacteriostasis for multiple pathogenic coccus and bacillus. obtain ethanol extraction from Radix seu Caulis Parthenocissi tricuspidatae decoct dry powder, further isolate the Radix seu Caulis Parthenocissi tricuspidatae element, its antibacterial action is stronger. and it is to staphylococcus aureus, Streptococcus viridans and Hemolytic streptococcus, streptococcus pneumoniae, micrococcus catarrhalis, diphtheria corynebacterium, escherichia coli, bacillus pyocyaneus, Bacillus typhi, Salmonella paratyphi, dysentery bacterium, Bacillus proteus, bordetella pertussis etc. have bacteriostasis when 0.002～0.63mg/ml, 0.005 be bactericidal action during～1.25mg/ml, mice whole blood, rabbit erythrocyte or serum can significantly reduce its antibacterial action.
Folium Meliae is Meliaceae deciduous tree plant, high 10-20m.The bark crineous, lobe, old branch purple, have most tiny hole skins.Be born in wilderness or roadside, often cultivate after the room anterior chamber.This plant grows rapidly on moistening fertile soil, not tight to the soil requirement, at acid soil, neutral soil and In Limestone Area, all can grow, and is that the other plantation of good reproducting tree species ， village wing of Plain and low height above sea level hills area is more suitable.This kind is not only material and used plant, is also medicinal plants, and its flower, leaf, fruit, root bark all can be used as medicine, and leaf cures mainly: heat clearing and damp drying; Killing parasites for relieving itching; Promoting the circulation of QI to relieve pain; The eczema pruritus; Skin ulcer tinea mange; Snake bite and insect sting; Trichomonal vaginitis; Hernia pain; Treating swelling and pain by traumatic injury.
Herba Houttuyniae has antibacterial action.The volatile oil components such as contained houttuynine sodium bisulfite, lauryl aldehyde, methyl n nonyl ketone, fragrant ethylene and quercitrin, cordarine in Herba Houttuyniae, staphylococcus aureus, Staphylococcus albus, dysentery bacterium, bacillus pyocyaneus, Bacillus proteus, paracolon, Grain-positive bacillus cereus etc. are all had to certain inhibitory action, stronger to staphylococcus aureus and Staphylococcus albus effect.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease and preparation method thereof.
Technical scheme: in order to solve the problems of the technologies described above, the invention provides a kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease, comprise following Chinese medicine, its parts by weight are respectively: 10 ~ 20 parts of Alternanthera philoxeroides, 20 ~ 30 parts of Herba Euphorbiae Humifusaes, 2 ~ 10 parts of Folium Meliaes, 15 ~ 25 parts of Herba Houttuyniae, 2 ~ 10 parts of the bright grass of Herba Cayatiae Japonicae, 10 ~ 20 parts of Radix Et Rhizoma Rhei.
Preferably, comprise following Chinese medicine, its parts by weight are respectively: 15 ~ 20 parts of Alternanthera philoxeroides, 25 ~ 30 parts of Herba Euphorbiae Humifusaes, 7 ~ 10 parts of Folium Meliaes, 20 ~ 25 parts of Herba Houttuyniae, 5 ~ 10 parts of the bright grass of Herba Cayatiae Japonicae, 15 ~ 20 parts of Radix Et Rhizoma Rhei.
Preferably, comprise following Chinese medicine, its parts by weight are respectively: 15 parts of Alternanthera philoxeroides, 25 parts of Herba Euphorbiae Humifusaes, 7 parts of Folium Meliaes, 20 parts of Herba Houttuyniae, 5 parts of the bright grass of Herba Cayatiae Japonicae, 15 parts of Radix Et Rhizoma Rhei.
The preparation method of the pharmaceutical composition of described treatment Fish white head and mouth fish disease comprises the following steps:
1) take 10 ~ 20 parts of ethanol that add 10 ~ 15 times 80% of Alternanthera philoxeroides, adopt supercritical CO
2extraction obtains liking the yerbadetajo seed effective ingredient;
2) take 20 ~ 30 parts of Herba Euphorbiae Humifusaes and add 75% ethanol of 10 ~ 15 times of amounts to carry out alcohol reflux, extract 5 times, each 2.5h obtains humifuse euphorbia effective ingredient;
3) take 10 ~ 20 parts of 2 ~ 10 parts of the bright grass of 2 ~ 10 parts of Folium Meliaes, Herba Cayatiae Japonicae, 15 ~ 25 parts of Herba Houttuyniae, Radix Et Rhizoma Rhei and add 20 ~ 25 times of decoctings to boil 2 ~ 4h, filter, obtain supernatant;
4) get happiness yerbadetajo seed effective ingredient, the step 2 of step 1)) humifuse euphorbia effective ingredient and the supernatant of step 3) mix the pharmaceutical composition of the Fish white head and mouth fish disease that obtains medical treatment.
Described humifuse euphorbia effective ingredient is total flavones.
Described supercritical CO
2extraction conditions is: 50 ~ 60 ℃ of temperature, pressure are that 27 ~ 30MPa, quiet hour are 2h, and dynamic time is 1.5h.
Beneficial effect: a kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease provided by the invention, wherein Herba Euphorbiae Humifusae, Folium Meliae, Herba Houttuyniae have antibiotic, antibacterial effect, these can prevent and treat white head and mouth fish disease well as effective ingredient, and treated effect is up to 99%.In preparation method of the present invention, Alternanthera philoxeroides extracts and has adopted supercritical CO
2extracting method, not only make the activity of Alternanthera philoxeroides effective ingredient all retain to some extent, and content is higher.Simple, the applicable large-scale production of preparation method of the present invention.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described.
embodiment 1:
A kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease, comprise following Chinese medicine, and its parts by weight are respectively: 10 parts of Alternanthera philoxeroides, 20 parts of Herba Euphorbiae Humifusaes, 2 parts of Folium Meliaes, 15 parts of Herba Houttuyniae, 2 parts of the bright grass of Herba Cayatiae Japonicae, 10 parts of Radix Et Rhizoma Rhei.
The preparation method of the pharmaceutical composition of described treatment Fish white head and mouth fish disease comprises the following steps:
1) take 10 parts of ethanol that add 10 times 80% of Alternanthera philoxeroides, adopt supercritical CO
2extraction obtains liking the yerbadetajo seed effective ingredient;
2) take 20 parts of Herba Euphorbiae Humifusaes and add 75% ethanol of 10 times of amounts to carry out alcohol reflux, extract 5 times, each 2.5h obtains humifuse euphorbia effective ingredient;
3) take 10 parts of 2 parts of the bright grass of 2 parts of Folium Meliaes, Herba Cayatiae Japonicae, 15 parts of Herba Houttuyniae, Radix Et Rhizoma Rhei and add 20 ~ 25 times of decoctings to boil 2h, filter, obtain supernatant;
4) get happiness yerbadetajo seed effective ingredient, the step 2 of step 1)) humifuse euphorbia effective ingredient and the supernatant of step 3) mix the pharmaceutical composition of the Fish white head and mouth fish disease that obtains medical treatment.
Described humifuse euphorbia effective ingredient is total flavones.
Described supercritical CO
2extraction conditions is: temperature 50 C, pressure are that 27MPa, quiet hour are 2h, and dynamic time is 1.5h.
embodiment 2:
A kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease, comprise following Chinese medicine, and its parts by weight are respectively: 20 parts of Alternanthera philoxeroides, 30 parts of Herba Euphorbiae Humifusaes, 10 parts of Folium Meliaes, 25 parts of Herba Houttuyniae, 10 parts of the bright grass of Herba Cayatiae Japonicae, 20 parts of Radix Et Rhizoma Rhei.
The preparation method of the pharmaceutical composition of described treatment Fish white head and mouth fish disease comprises the following steps:
1) take 20 parts of ethanol that add 15 times 80% of Alternanthera philoxeroides, adopt supercritical CO
2extraction obtains liking the yerbadetajo seed effective ingredient;
2) take 30 parts of Herba Euphorbiae Humifusaes and add 75% ethanol of 15 times of amounts to carry out alcohol reflux, extract 5 times, each 2.5h obtains humifuse euphorbia effective ingredient;
3) take 20 parts of 10 parts of the bright grass of 10 parts of Folium Meliaes, Herba Cayatiae Japonicae, 25 parts of Herba Houttuyniae, Radix Et Rhizoma Rhei and add 25 times of decoctings to boil 4h, filter, obtain supernatant;
4) get happiness yerbadetajo seed effective ingredient, the step 2 of step 1)) humifuse euphorbia effective ingredient and the supernatant of step 3) mix the pharmaceutical composition of the Fish white head and mouth fish disease that obtains medical treatment.
Described humifuse euphorbia effective ingredient is total flavones.
Described supercritical CO
2extraction conditions is: temperature 60 C, pressure are that 30MPa, quiet hour are 2h, and dynamic time is 1.5h.
embodiment 3:
A kind of pharmaceutical composition for the treatment of the Fish white head and mouth fish disease, comprise following Chinese medicine, and its parts by weight are respectively: 15 parts of Alternanthera philoxeroides, 25 parts of Herba Euphorbiae Humifusaes, 7 parts of Folium Meliaes, 20 parts of Herba Houttuyniae, 5 parts of the bright grass of Herba Cayatiae Japonicae, 15 parts of Radix Et Rhizoma Rhei.
The preparation method of the pharmaceutical composition of described treatment Fish white head and mouth fish disease comprises the following steps:
1) take 15 parts of ethanol that add 13 times 80% of Alternanthera philoxeroides, adopt supercritical CO
2extraction obtains liking the yerbadetajo seed effective ingredient;
2) take 25 parts of Herba Euphorbiae Humifusaes and add 75% ethanol of 13 times of amounts to carry out alcohol reflux, extract 5 times, each 2.5h obtains humifuse euphorbia effective ingredient;
3) take 15 parts of 5 parts of the bright grass of 7 parts of Folium Meliaes, Herba Cayatiae Japonicae, 20 parts of Herba Houttuyniae, Radix Et Rhizoma Rhei and add 23 times of decoctings to boil 3h, filter, obtain supernatant;
4) get happiness yerbadetajo seed effective ingredient, the step 2 of step 1)) humifuse euphorbia effective ingredient and the supernatant of step 3) mix the pharmaceutical composition of the Fish white head and mouth fish disease that obtains medical treatment.
Described humifuse euphorbia effective ingredient is total flavones.
Described supercritical CO
2extraction conditions is: 55 ℃ of temperature, pressure are that 28MPa, quiet hour are 2h, and dynamic time is 1.5h.
The embodiment of the present invention 1 ~ 3 any one pharmaceutical composition usage and consumption: full pool spilling head, every 1 cubic metre of water body medication 1.2 ~ 2.8g, every mu of pharmaceutical composition 1400 ~ 1700g of the present invention for water body depth of water 1m, be used in conjunction 3 days.
Experimental condition:
The category-A pond: 2 mu of areas, 1.7 meters of depth of water, water source is that Yangtze River Water, pH are 7.0, water temperature is 25 ℃.It is 94g that this pond Mesichthyes has Ctenopharyngodon idellus, Carassius auratus, Cyprinus carpio, each 100 of Hypophthalmichthys molitrixs, average weight.
The category-B pond: 1.9 mu of areas, 1.6 meters of depth of water, water source is that Yangtze River Water, pH are 7.0, water temperature is 25 ℃.It is 95g that this pond Mesichthyes has Ctenopharyngodon idellus, Carassius auratus, Cyprinus carpio, each 100 of Hypophthalmichthys molitrixs, average weight.
Test method:
Category-A pond medication 4kg, do not have dead fish, second day medication 4kg, and the white head and mouth fish disease of fish is improved, the 3rd day medication 4kg, the Fish white head and mouth fish disease is cured, and moves about freely.
The category-B pond does not have medication, 15 of the dead fishes of first day, and 33 of the dead fishes of second day, within the 3rd day, dead fish is 57.
Result of the test:
The category-A pond does not have dead fish, and cure rate is 100%, the not medication of category-B pond, and mortality rate increases.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. a pharmaceutical composition for the treatment of the Fish white head and mouth fish disease, it is characterized in that, comprise following Chinese medicine, its parts by weight are respectively: 10 ~ 20 parts of Alternanthera philoxeroides, 20 ~ 30 parts of Herba Euphorbiae Humifusaes, 2 ~ 10 parts of Folium Meliaes, 15 ~ 25 parts of Herba Houttuyniae, 2 ~ 10 parts of the bright grass of Herba Cayatiae Japonicae, 10 ~ 20 parts of Radix Et Rhizoma Rhei.
2. pharmaceutical composition according to claim 1, it is characterized in that, comprise following Chinese medicine, its parts by weight are respectively: 15 ~ 20 parts of Alternanthera philoxeroides, 25 ~ 30 parts of Herba Euphorbiae Humifusaes, 7 ~ 10 parts of Folium Meliaes, 20 ~ 25 parts of Herba Houttuyniae, 5 ~ 10 parts of the bright grass of Herba Cayatiae Japonicae, 15 ~ 20 parts of Radix Et Rhizoma Rhei.
3. pharmaceutical composition according to claim 1, is characterized in that, comprises following Chinese medicine, and its parts by weight are respectively: 15 parts of Alternanthera philoxeroides, 25 parts of Herba Euphorbiae Humifusaes, 7 parts of Folium Meliaes, 20 parts of Herba Houttuyniae, 5 parts of the bright grass of Herba Cayatiae Japonicae, 15 parts of Radix Et Rhizoma Rhei.
4. the preparation method of the pharmaceutical composition for the treatment of Fish white head and mouth fish disease claimed in claim 1, is characterized in that, comprises the following steps:
1) take 10 ~ 20 parts of ethanol that add 10 ~ 15 times 80% of Alternanthera philoxeroides, adopt supercritical CO
2extraction obtains liking the yerbadetajo seed effective ingredient;
2) take 20 ~ 30 parts of Herba Euphorbiae Humifusaes and add 75% ethanol of 10 ~ 15 times of amounts to carry out alcohol reflux, extract 5 times, each 2.5h obtains humifuse euphorbia effective ingredient;
3) take 10 ~ 20 parts of 2 ~ 10 parts of the bright grass of 2 ~ 10 parts of Folium Meliaes, Herba Cayatiae Japonicae, 15 ~ 25 parts of Herba Houttuyniae, Radix Et Rhizoma Rhei and add 20 ~ 25 times of decoctings to boil 2 ~ 4h, filter, obtain supernatant;
4) get happiness yerbadetajo seed effective ingredient, the step 2 of step 1)) humifuse euphorbia effective ingredient and the supernatant of step 3) mix the pharmaceutical composition of the Fish white head and mouth fish disease that obtains medical treatment.
5. the preparation method of pharmaceutical composition according to claim 4, described humifuse euphorbia effective ingredient is total flavones.
6. the preparation method of pharmaceutical composition according to claim 4, is characterized in that described supercritical CO
2extraction conditions is: 50 ~ 60 ℃ of temperature, pressure are that 27 ~ 30MPa, quiet hour are 2h, and dynamic time is 1.5h.
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| CN201310342893.3A CN103463317B (en) | 2013-08-08 | 2013-08-08 | A kind of pharmaceutical composition for treating fish white head and mouth fish disease and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103933184A (en) * | 2014-04-22 | 2014-07-23 | 湖州天健兽药有限公司 | Traditional Chinese medicine composition for treating white head-mouth disease of fishes |
| CN104398556A (en) * | 2014-12-08 | 2015-03-11 | 鼎正动物药业（天津）有限公司 | Traditional Chinese medicine compound for preventing and controlling white head-mouth disease of grass carp fries and preparation method |
| CN106581506A (en) * | 2017-03-06 | 2017-04-26 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating white head-mouth disease of fish |
| CN106729114A (en) * | 2017-03-06 | 2017-05-31 | 长沙瑞多康生物科技有限公司 | A kind of composition for preventing and treating fish white head and mouth fish disease |
-
2013
- 2013-08-08 CN CN201310342893.3A patent/CN103463317B/en active Active
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| 刘艳华等: "中草药防治鱼病研究进展", 《中国兽药杂志》 * |
| 朱九根等: "细菌性鱼病的中草药防治研究初报", 《江西农业学报》 * |
| 郑继方等: "《中草药饲料添加剂的配制与应用》", 31 October 2007, 金盾出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103933184A (en) * | 2014-04-22 | 2014-07-23 | 湖州天健兽药有限公司 | Traditional Chinese medicine composition for treating white head-mouth disease of fishes |
| CN103933184B (en) * | 2014-04-22 | 2016-08-31 | 湖州天健兽药有限公司 | A kind of Chinese medicine composition treating Fish white head and mouth fish disease |
| CN104398556A (en) * | 2014-12-08 | 2015-03-11 | 鼎正动物药业（天津）有限公司 | Traditional Chinese medicine compound for preventing and controlling white head-mouth disease of grass carp fries and preparation method |
| CN106581506A (en) * | 2017-03-06 | 2017-04-26 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating white head-mouth disease of fish |
| CN106729114A (en) * | 2017-03-06 | 2017-05-31 | 长沙瑞多康生物科技有限公司 | A kind of composition for preventing and treating fish white head and mouth fish disease |
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| CN103463317B (en) | 2017-11-07 |
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