CN103463317B - A kind of pharmaceutical composition for treating fish white head and mouth fish disease and preparation method thereof - Google Patents
A kind of pharmaceutical composition for treating fish white head and mouth fish disease and preparation method thereof Download PDFInfo
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- CN103463317B CN103463317B CN201310342893.3A CN201310342893A CN103463317B CN 103463317 B CN103463317 B CN 103463317B CN 201310342893 A CN201310342893 A CN 201310342893A CN 103463317 B CN103463317 B CN 103463317B
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Abstract
The invention discloses a kind of pharmaceutical composition for treating fish white head and mouth fish disease, comprising following Chinese medicine, its parts by weight is respectively：10 ~ 20 parts of Alternanthera philoxeroides, 20 ~ 30 parts of wolf's milk, 2 ~ 10 parts of Folium Meliae, 15 ~ 25 parts of cordate houttuynia, 2 ~ 10 parts of Japanese Cayratia Herb fresh grass, 10 ~ 20 parts of rheum officinale.A kind of pharmaceutical composition for treating fish white head and mouth fish disease that the present invention is provided, wherein wolf's milk, Folium Meliae, cordate houttuynia have antibacterial, antibacterial effect, and these can prevent and treat white head and mouth fish disease well as active ingredient, and treated effect is up to 99%.Alternanthera philoxeroides extracts and employs supercritical CO in the preparation method of the present invention2Extracting method, not only causes the activity of Alternanthera philoxeroides active ingredient all to retain, and content is higher.Preparation method of the invention is simple and easy to apply, be adapted to large-scale production.
Description
Technical field
The present invention relates to the field of Chinese medicines for the treatment of fish disease, and in particular to a kind of medicine for treating fish white head and mouth fish disease
Composition and preparation method thereof.
Background technology
White head and mouth fish disease is by myxococcus（Myxococcus）The caused bacterial diseases of fishes.Main harm summer flower fingerling.Sick fish
It is creamy white at kiss end to eyeball, lip swelling is opened and closes failure, is had difficulty in breathing, and mouth surrounding skin is rotten to the corn into cotton-shaped.Morbidity
When, the meronecrosis around the forehead and mouth of sick fish, pigment disappears and shows white, and diseased region festers, sometimes band
There is canescence villiform thing, thus " the white mouth of hoary hair " symptom is presented.The disease fish moved about in the water surface, symptom is particularly evident.When sick fish
After water, symptom is not just notable.Serious sick fish, lesions position festers, and congested phenomenon occur in indivedual disease fish head portions, sometimes
Also show the reaction of the lesions such as white skin, white smear, rotten tail, gill rot or whole body many mucus.The sick general body of fish is thin, black, accelerated breathing, food
It is intended to depressed, swimming is slow, constantly emerges, soon i.e. death.This disease is a kind of eruptive epidemic disease, and morbidity is exceedingly fast, and is infected
Rapidly, can be all dead between one day.This sick epidemic season is obvious, typically in late May to early July, and June is hair
Sick peak.
Alternanthera philoxeroides already as a kind of Chinese medicine include into《Chinese Pharmacopoeia》, the careless bitter is cold in nature, nontoxic, have heat-clearing,
Cool blood, the function of removing toxic substances, cure mainly the illnesss such as mass formed by blood stasis, venomous snake bite, stranguria with turbid discharge, furunculosis, eczema.Alternanthera philoxeroides is to Gram-positive
There is significant antibacterial action with negative bacteria, can substantially suppress meningococcus, Pseudomonas aeruginosa, staphylococcus aureus, white
The breeding of larynx bacillus, lung diplococcoid, viridans streptococci, bacterium flexneri, yeast bacillus etc..
The fresh juice of wolf's milk, decoction and water boiling concentration ethanol extract have obvious suppression for a variety of pathogenic coccuses and bacillus
Bacterium effect obtains ethanol extract from humid euphorbia decoction dry powder, is further separated out humid euphorbia element, it is right by the stronger of its antibacterial action
Staphylococcus aureus, Streptococcus viridans and hemolytic streptococcus, pneumococcus, micrococcus catarrhalis, corynebacterium diphtheriae, large intestine bar
Bacterium, Pseudomonas aeruginosa, typhoid bacillus, paratyphosum Bacterium, shigella dysenteriae, proteus, Bordetella pertussis etc. 0.002~
There is bacteriostasis during 0.63mg/ml, be in bactericidal action during 0.005~1.25mg/ml；Mouse whole blood, rabbit erythrocyte or serum
Its antibacterial action can be significantly reduced.
Folium Meliae is Meliaceae deciduous tree plant, high 10-20m.Bark crineous, lobe, old branch purple has most tiny
Hole skin.Wilderness or roadside are born in, is often cultivated in after room anterior chamber.The plant grows rapidly on the fertile soil of moistening, to soil requirement
It is not tight, it can be grown in acid soil, neutral soil and In Limestone Area, be the good reproducting tree species of Plain and low altitude area hills area,
Plant more suitable by village's wing.This kind is not only timber-used plant, is also medicinal plant, its flower, leaf, fruit, root skin
It is used as medicine, leaf is cured mainly：Heat-clearing and damp-drying drug；Desinsection is antipruritic；Promoting qi circulation and relieving pain；Eczema itch；Sore, tinea, scabies and leprosy；Snake bite and insect sting；Trichomonas vagina
It is scorching；Hernia pain；Treating swelling and pain by traumatic injury.
Cordate houttuynia has antibacterial action.Contained decanoy acetaldehyde in cordate houttuynia, lauryl aldehyde, methyl n nonyl ketone, fragrant ethene and
The volatile oil components such as quercitrin, cordarine, to staphylococcus aureus, staphylococcus albus, shigella dysenteriae, Pseudomonas aeruginosa, change
Shape bacillus, paracolon, Grain-positive bacillus etc. have certain inhibitory action, to staphylococcus aureus and white Portugal
The effect of grape coccus is stronger.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the present invention provides one kind and treats the white mouth of fish hoary hair
Pharmaceutical composition of disease and preparation method thereof.
Technical scheme：In order to solve the above-mentioned technical problem, the invention provides a kind of medicine for treating fish white head and mouth fish disease
Compositions, comprising following Chinese medicine, its parts by weight is respectively：10 ~ 20 parts of Alternanthera philoxeroides, 20 ~ 30 parts of wolf's milk, Folium Meliae
2 ~ 10 parts, 15 ~ 25 parts of cordate houttuynia, 2 ~ 10 parts of Japanese Cayratia Herb fresh grass, 10 ~ 20 parts of rheum officinale.
Preferably, comprising following Chinese medicine, its parts by weight is respectively：15 ~ 20 parts of Alternanthera philoxeroides, 25 ~ 30 parts of wolf's milk,
7 ~ 10 parts of Folium Meliae, 20 ~ 25 parts of cordate houttuynia, 5 ~ 10 parts of Japanese Cayratia Herb fresh grass, 15 ~ 20 parts of rheum officinale.
Preferably, comprising following Chinese medicine, its parts by weight is respectively：15 parts of Alternanthera philoxeroides, 25 parts of wolf's milk, Folium Meliae
7 parts, 20 parts of cordate houttuynia, 5 parts of Japanese Cayratia Herb fresh grass, 15 parts of rheum officinale.
The preparation method of the pharmaceutical composition of described treatment fish white head and mouth fish disease, comprises the following steps：
1）Weigh 10 ~ 20 parts of Alternanthera philoxeroides and Jia 10 ~ 15 times 80% of ethanol, using supercritical CO2Extraction obtains liking non-irrigated lotus
Sub- active ingredient；
2）The 75% ethanol progress alcohol reflux extraction that 20 ~ 30 parts of wolf's milk adds 10 ~ 15 times of amounts is weighed, is extracted 5 times,
Each 2.5h obtains humifuse euphorbia effective ingredient；
3）Weigh 2 ~ 10 parts of Folium Meliae, 2 ~ 10 parts of Japanese Cayratia Herb fresh grass, 15 ~ 25 parts of cordate houttuynia, 10 ~ 20 parts of rheum officinale add 20 ~
2 ~ 4h is boiled in 25 times of decoctings, filtering, obtains supernatant；
4）Take step 1）Happiness yerbadetajo seed active ingredient, step 2）Humifuse euphorbia effective ingredient and step 3）Supernatant
Mix the pharmaceutical composition for the fish white head and mouth fish disease that obtains medical treatment.
The humifuse euphorbia effective ingredient is general flavone.
The supercritical CO2Extraction conditions are：50 ~ 60 DEG C of temperature, pressure be 27 ~ 30MPa, quiet hour be 2h, dynamic
Time is 1.5h.
Beneficial effect：A kind of pharmaceutical composition for treating fish white head and mouth fish disease that the present invention is provided, wherein wolf's milk, hardship
Chinaberry leaf, cordate houttuynia have antibacterial, antibacterial effect, and these can prevent and treat the white mouth of hoary hair well as active ingredient
Disease, treated effect is up to 99%.Alternanthera philoxeroides extracts and employs supercritical CO in the preparation method of the present invention2Extracting method,
Not only so that the activity of Alternanthera philoxeroides active ingredient has all retained, and content is higher.The preparation method of the present invention is simple
Easy, suitable large-scale production.
Embodiment
The present invention is further described with reference to embodiment.
Embodiment 1：
A kind of pharmaceutical composition for treating fish white head and mouth fish disease, comprising following Chinese medicine, its parts by weight is respectively：Happiness drought
10 parts of alternanthera, 20 parts of wolf's milk, 2 parts of Folium Meliae, 15 parts of cordate houttuynia, 2 parts of Japanese Cayratia Herb fresh grass, 10 parts of rheum officinale.
The preparation method of the pharmaceutical composition of described treatment fish white head and mouth fish disease, comprises the following steps：
1）The ethanol of 10 parts plus 10 times 80% of Alternanthera philoxeroides is weighed, using supercritical CO2It is effective that extraction obtains happiness yerbadetajo seed
Composition；
2）Weigh 20 parts of wolf's milk and add 75% ethanol of 10 times of amounts and carry out alcohol reflux extractions, extract 5 times, every time
2.5h obtains humifuse euphorbia effective ingredient；
3）Weigh 2 parts of Folium Meliae, 2 parts of Japanese Cayratia Herb fresh grass, 15 parts of cordate houttuynia, 10 parts of rheum officinale and add 20 ~ 25 times of decoctings and boil 2h,
Filtering, obtains supernatant；
4）Take step 1）Happiness yerbadetajo seed active ingredient, step 2）Humifuse euphorbia effective ingredient and step 3）Supernatant
Mix the pharmaceutical composition for the fish white head and mouth fish disease that obtains medical treatment.
The humifuse euphorbia effective ingredient is general flavone.
The supercritical CO2Extraction conditions are：Temperature 50 C, pressure be 27MPa, quiet hour be 2h, dynamic time is
1.5h。
Embodiment 2：
A kind of pharmaceutical composition for treating fish white head and mouth fish disease, comprising following Chinese medicine, its parts by weight is respectively：Happiness drought
20 parts of alternanthera, 30 parts of wolf's milk, 10 parts of Folium Meliae, 25 parts of cordate houttuynia, 10 parts of Japanese Cayratia Herb fresh grass, 20 parts of rheum officinale.
The preparation method of the pharmaceutical composition of described treatment fish white head and mouth fish disease, comprises the following steps：
1）The ethanol of 20 parts plus 15 times 80% of Alternanthera philoxeroides is weighed, using supercritical CO2It is effective that extraction obtains happiness yerbadetajo seed
Composition；
2）Weigh 30 parts of wolf's milk and add 75% ethanol of 15 times of amounts and carry out alcohol reflux extractions, extract 5 times, every time
2.5h obtains humifuse euphorbia effective ingredient；
3）Weigh 10 parts of Folium Meliae, 10 parts of Japanese Cayratia Herb fresh grass, 25 parts of cordate houttuynia, 20 parts of rheum officinale and add 25 times of decoctings and boil 4h,
Filtering, obtains supernatant；
4）Take step 1）Happiness yerbadetajo seed active ingredient, step 2）Humifuse euphorbia effective ingredient and step 3）Supernatant
Mix the pharmaceutical composition for the fish white head and mouth fish disease that obtains medical treatment.
The humifuse euphorbia effective ingredient is general flavone.
The supercritical CO2Extraction conditions are：Temperature 60 C, pressure be 30MPa, quiet hour be 2h, dynamic time is
1.5h。
Embodiment 3：
A kind of pharmaceutical composition for treating fish white head and mouth fish disease, comprising following Chinese medicine, its parts by weight is respectively：Happiness drought
15 parts of alternanthera, 25 parts of wolf's milk, 7 parts of Folium Meliae, 20 parts of cordate houttuynia, 5 parts of Japanese Cayratia Herb fresh grass, 15 parts of rheum officinale.
The preparation method of the pharmaceutical composition of described treatment fish white head and mouth fish disease, comprises the following steps：
1）The ethanol of 15 parts plus 13 times 80% of Alternanthera philoxeroides is weighed, using supercritical CO2It is effective that extraction obtains happiness yerbadetajo seed
Composition；
2）Weigh 25 parts of wolf's milk and add 75% ethanol of 13 times of amounts and carry out alcohol reflux extractions, extract 5 times, every time
2.5h obtains humifuse euphorbia effective ingredient；
3）Weigh 7 parts of Folium Meliae, 5 parts of Japanese Cayratia Herb fresh grass, 20 parts of cordate houttuynia, 15 parts of rheum officinale and add 23 times of decoctings and boil 3h, mistake
Filter, obtains supernatant；
4）Take step 1）Happiness yerbadetajo seed active ingredient, step 2）Humifuse euphorbia effective ingredient and step 3）Supernatant
Mix the pharmaceutical composition for the fish white head and mouth fish disease that obtains medical treatment.
The humifuse euphorbia effective ingredient is general flavone.
The supercritical CO2Extraction conditions are：55 DEG C of temperature, pressure be 28MPa, quiet hour be 2h, dynamic time is
1.5h。
The pharmaceutical composition usage and consumption of any one of the embodiment of the present invention 1 ~ 3：Full pool spilling head, every 1 cubic metre of water body
Medication 1.2 ~ 2.8g, every mu of water body depth of water 1m 1400 ~ 1700g of pharmaceutical composition of the present invention, are used in conjunction 3 days.
Experimental condition：
A classes pond：2 mu of area, 1.7 meters of depth of water, water source are that Yangtze River Water, pH are 7.0, and water temperature is 25 DEG C.The pond
Mesichthyes has each 100 of grass carp, crucian, carp, silver carp, average weight to be 94g.
B classes pond：1.9 mu of area, 1.6 meters of depth of water, water source are that Yangtze River Water, pH are 7.0, and water temperature is 25 DEG C.The pond
Pool Mesichthyes has each 100 of grass carp, crucian, carp, silver carp, average weight to be 95g.
Test method：
A classes pond medication 4kg, without dead fish, second day medication 4kg, the white head and mouth fish disease of fish improves, medication in the 3rd day
4kg, fish white head and mouth fish disease is cured, and is moved about freely.
B classes pond does not have medication, first day dead fish 15, second day dead fish 33, the 3rd day 57 articles of dead fish.
Result of the test：
A classes pond does not have dead fish, and cure rate is the non-medication in 100%, B classes pond, and the death rate increases.
Described above is only the preferred embodiment of the present invention, it should be pointed out that：Come for those skilled in the art
Say, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (4)
1. a kind of pharmaceutical composition for treating fish white head and mouth fish disease, it is characterised in that be made up of following Chinese medicine, its parts by weight
Respectively：10 ~ 20 parts of Alternanthera philoxeroides, 20 ~ 30 parts of wolf's milk, 2 ~ 10 parts of Folium Meliae, 15 ~ 25 parts of cordate houttuynia, Japanese Cayratia Herb
2 ~ 10 parts of fresh grass, 10 ~ 20 parts of rheum officinale.
2. the pharmaceutical composition according to claim 1, it is characterised in that be made up of following Chinese medicine, its parts by weight difference
For：15 ~ 20 parts of Alternanthera philoxeroides, 25 ~ 30 parts of wolf's milk, 7 ~ 10 parts of Folium Meliae, 20 ~ 25 parts of cordate houttuynia, Japanese Cayratia Herb fresh grass 5
~ 10 parts, 15 ~ 20 parts of rheum officinale.
3. the pharmaceutical composition according to claim 1, it is characterised in that be made up of following Chinese medicine, its parts by weight difference
For：15 parts of Alternanthera philoxeroides, 25 parts of wolf's milk, 7 parts of Folium Meliae, 20 parts of cordate houttuynia, 5 parts of Japanese Cayratia Herb fresh grass, rheum officinale 15
Part.
4. the preparation method of the pharmaceutical composition of the treatment fish white head and mouth fish disease described in claim 1, it is characterised in that bag
Include following steps：
1）Weigh 10 ~ 20 parts of Alternanthera philoxeroides and Jia 10 ~ 15 times 80% of ethanol, using supercritical CO2 Extraction obtains liking non-irrigated lotus
Sub- active ingredient；The supercritical CO2Extraction conditions are：50 ~ 60 DEG C of temperature, pressure be 27 ~ 30MPa, quiet hour be 2h,
Dynamic time is 1.5h；
2）The 75% ethanol progress alcohol reflux extraction that 20 ~ 30 parts of wolf's milk adds 10 ~ 15 times of amounts is weighed, is extracted 5 times,
Each 2.5h obtains humifuse euphorbia effective ingredient；The humifuse euphorbia effective ingredient is general flavone；
3）Weigh 2 ~ 10 parts of Folium Meliae, 2 ~ 10 parts of Japanese Cayratia Herb fresh grass, 15 ~ 25 parts of cordate houttuynia, 10 ~ 20 parts of rheum officinale add 20 ~
2 ~ 4h is boiled in 25 times of decoctings, filtering, obtains supernatant；
4）Take step 1）Happiness yerbadetajo seed active ingredient, step 2）Humifuse euphorbia effective ingredient and step 3）Supernatant mixing
The pharmaceutical composition for the fish white head and mouth fish disease that obtains medical treatment.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310342893.3A CN103463317B (en) | 2013-08-08 | 2013-08-08 | A kind of pharmaceutical composition for treating fish white head and mouth fish disease and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310342893.3A CN103463317B (en) | 2013-08-08 | 2013-08-08 | A kind of pharmaceutical composition for treating fish white head and mouth fish disease and preparation method thereof |
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| CN103463317B true CN103463317B (en) | 2017-11-07 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103933184B (en) * | 2014-04-22 | 2016-08-31 | 湖州天健兽药有限公司 | A kind of Chinese medicine composition treating Fish white head and mouth fish disease |
| CN104398556A (en) * | 2014-12-08 | 2015-03-11 | 鼎正动物药业（天津）有限公司 | Traditional Chinese medicine compound for preventing and controlling white head-mouth disease of grass carp fries and preparation method |
| CN106581506A (en) * | 2017-03-06 | 2017-04-26 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating white head-mouth disease of fish |
| CN106729114A (en) * | 2017-03-06 | 2017-05-31 | 长沙瑞多康生物科技有限公司 | A kind of composition for preventing and treating fish white head and mouth fish disease |
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- 2013-08-08 CN CN201310342893.3A patent/CN103463317B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 中草药防治鱼病研究进展;刘艳华等;《中国兽药杂志》;19981231;第32卷(第1期);第52页右栏第2.1节第2段，第53页左栏第1段 * |
| 细菌性鱼病的中草药防治研究初报;朱九根等;《江西农业学报》;20081231;第20卷(第2期);第95-96页 * |
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