Background technology
Ganoderma be the Basidiomycetes Polyporaceae (
polyporaceae) Ganoderma (
ganodelma) the fungus Ganoderma lucidum (Leyss. Ex Fr.) Karst. (
g.lucidumkarst).Wield research and show, the pharmaceutical component of Ganoderma is very abundant, and wherein effective ingredient can be divided into ten large classes, comprise ganoderan, Ganoderma polypeptide, triterpenes, 16 seed amino acids (being mainly Fumaric acid) and trace element every, ferrum, calcium, suddenly, zinc etc.Ganoderan is one of main effective ingredient of Ganoderma, and it is the main component of Ganoderma strengthening the body resistance, strengthening by means of tonics, life lengthening.Ganoderma is two-ways regulation to the effect of human body, its power relates to each systems of human body such as cardiovascular and cerebrovascular vessel, digestion, breathing, endocrine, nerve, motion, and Ganoderma also has antitumor, immunomodulating, blood sugar lowering, antioxidation, blood fat reducing and anti-aging effects.
Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae), the Latin formal name used at school:
corydalis saxicola Bunting, belong to the Papaverales Papaveraceae, another name: rock Hu, rock company, Radix corydalis pallidae, Radix seu caulis Berberidis Gagnepainii (Radix Berberidis Virgetori), herbaceos perennial, high 15-40cm, the main root prosperity, be born in the border rock slot of mountain region, is distributed in the ground such as Gansu, Hubei, Guangxi, Sichuan, Guizhou, Yunnan.Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) is also a kind of Chinese medicine, has the multiple curative effects such as heat-clearing and toxic substances removing, treatment hepatitis; Attending effectiveness: heat-clearing and toxic substances removing; Dampness removing; Analgesic hemostatic; Cure mainly hepatitis; Aphthous stomatitis; Acute catarrhal conjunctivitis; Cough, corneal nebula; Dysentery; Diarrhoea; Stomachache; Bleeding hemorrhoids, also have certain antitumaous effect.It is documented, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) contains multiple alkaloid.Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) is due to above-mentioned heat-clearing and toxic substances removing, dampness removing, analgesic hemostatic function and chemical composition being arranged, it is found that it has special efficacy to the liver disease, the multi-efficiency that there is anti-hepatitis virus simultaneously and protect the liver, thereby be widely used in the treatment of hepatitis, liver cirrhosis, hepatocarcinoma, and obtain beyond thought special effect, Guangxi, some pharmaceutical factories, Guizhou have produced some for oral administration and ejection preparations with Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) at present, do not find toxic and side effects through long-term taking and intravenous injection, so Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) is as the important drugs for the treatment of hepatic disease.
Fructus Lycii, the Latin formal name used at school:
lycium, belong to Lycium, Chinese Fructus Lycii, a lot of calls among the people are arranged, as red as Gou Qizi, Fructus Lycii real, Radix Betae is sub, FRUCTUS LYCII BARBARI, Canis familiaris L. milk, frame, red ear pendant, blood Fructus Lycii, Chinese holly ground bud, fructus lycii sinensis." Book of Songs " of this title of Fructus Lycii before first appeared in China's two thousand years.The medicine scholar Li Shizhen (1518-1593 A.D.) cloud of the Ming Dynasty: " Fructus Lycii, two tree names.This thing sour jujube is as the thorn of Chinese holly, and stem is as the bar of Qi, therefore the name of holding concurrently ".Road book speech: thousand years Fructus Lyciis, its shape is as dog, therefore obtain the Chinese holly name.Song is said: the Caulis Bambusae has three kinds: the one, and Fructus Lycii; The one, the dish class, leaf is like Herba Lactucae versicoloris; The one, the darkish complexion person of withered bamboo pole also.Fructus Lycii, to the influential effect of the function of exempting from service, shows as nourishing the liver, nourishing kidney, lung moistening; Main component: lycium barbarum polysaccharide, wolfberry pigment, betanin (
betane), atropine (
atropine), hyoscyamine (
gyoscyamine).Fructus Lycii is the comparison temperature compensation, and property is flat, sweet in the mouth, have the kidney invigorating nourishing the liver, replenishing vital essence to improve eyesight, strengthening bone and muscle, except lumbago, for a long time clothes can life prolonging etc. function.Although but Fructus Lycii is that the excellent nourishing of a kind of drug effect is protected and built treasure, the effectiveness of its warm health is fairly obvious, for hyperpietic, irascible temperament person, or overnutrition person being not suitable for.
Fructus Momordicae, the Latin formal name used at school:
momordica grosvenori Swingle,for cucurbitaceous plant, Fructus Momordicae belongs to the herbaceous perennial vine plant, the dry mature fruit of Fructus Momordicae.Fructus Momordicae is described as " angle fruit " by people.Mainly originate in the famous and precious local and special products in ,Shi Guilin, mountain area of Lingui district, Guilin City, Guangxi Zhuang Autonomous Region and Yongfu county.Fruits nutrition is worth very high, contains abundant vitamin C (in every 100 gram fresh fruits, containing 400 milligrams~500 milligrams) and glucoside, fructose, glucose, protein, lipid etc.For a kind of Chinese medicine commonly used, there is the effect that clearing away summer-heat, lung moistening quench the thirst, also the various diseases such as hypertension, pulmonary tuberculosis, asthma, hepatitis, bronchitis and tonsillitis there is is the effect for the treatment of.
Summary of the invention
The object of the invention is to the drug effect separately for Ganoderma, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae), Fructus Lycii, Fructus Momordicae, and a kind of simple formula combination is provided, allow its drug effect be improved and symphyogenetic a kind of chewable tablet.
The present invention is achieved in that
The composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae), its composition comprises the raw material of following parts by weight:
10~25 parts of Ganodermas; Fructus Lycii 10~15; Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) 2-6 part; 5~10 parts of Fructus Momordicaes; 20~30 parts of hydroxypropyl emthylcelluloses; 5~15 parts of maltodextrins; Lubricant 1~3.
Described Fructus Momordicae, English name: Fructus Momordicae (drawing); The dry fruit of grosvenor momordica fruit cucurbitaceous plant Fructus Momordicae Siraitia grosvenorii (Swingle) C. Jeffrey ex Lu et Z. Y. Zhang.The Fructus Momordicae sweet in the mouth is cool in nature, returns lung, large intestine channel, and nourishing the lung to arrest cough is arranged, and the effect of promoting the production of body fluid to quench thirst is applicable to lung-heat or xeropulmonary cough, and pertussis and summer-heat impairment of body fluid are thirsty etc., also has in addition the effect of loosening bowel to relieve constipation.Fructus Momordicae is containing a kind of than the sweetener of sweet 300 times of sucrose, but it does not produce heat, so be the desirable replacement beverage of the unsuitable sugar eater such as diabetes, obesity.
Described lubricant is the compositions of any or they of Pulvis Talci, magnesium stearate, PEG4000 or PEG6000.
The preparation method of the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae), at first raw material Ganoderma, Fructus Lycii, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) and Fructus Momordicae are carried out respectively to the raw material processing, then proportionally add adjuvant, mix homogeneously, adopt the ethanol wet granulation, make the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with the tablet machine tabletting after adding lubricant;
It is that Ganoderma is cleaned with clear water that described Ganoderma raw material is processed, and is cut into 1~3cm fritter, and 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Ganoderma superfine powder, package spare;
It is that Fructus Lycii is cleaned with clear water that described Fructus Lycii raw material is processed, and 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Fructus Lycii superfine powder, package spare;
It is that Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) is cleaned with clear water that described Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) raw material is processed, and is cut into 1~3cm fritter, and 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, package spare;
It is that Fructus Momordicae is cleaned with clear water that described Fructus Momordicae raw material is processed, and is cut into 1~3cm fritter, and 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Fructus Momordicae superfine powder, package spare.
Described ethanol wet granulation, be various mixing of materials evenly after, add 60~85%(v/v) ethanol, mix homogeneously, the addition of ethanol to material have gentle hands is held agglomerating, looses one's grip and scatters; Make 20 purpose granules with screen cloth, then under 60~80 ℃, dry moisture content processed is 4~6%; Be cooled to room temperature, cross 60 mesh sieves.
While producing the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae), the pressure of tablet machine is 2.5~4MPa
The invention has the beneficial effects as follows:
1. the chewable tablet of making by combining four Chinese medicines, solve the problem that single herbal medicine efficacy can not work fully effectively.
2. the raw material such as Ganoderma, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae), become superfine powder by the superfine powder apparatus processing, directly uses, and its active ingredient can fully be absorbed by human body, plays a role.
3. this chewable tablet has heat-clearing and toxic substances removing, liver protecting and nourishing, immunomodulating, blood sugar lowering, antioxidation, blood fat reducing, defying age, replenishing vital essence to improve eyesight, strengthening bone and muscle, removes the effects such as lumbago, life prolonging.
4, the present invention's preferred Ganoderma with health role, Fructus Lycii, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) and Fructus Momordicae under Chinese medical theory instructs is raw material, science compatibility, making in addition, make that each raw material cooperatively interacts, learns from other's strong points to offset one's weaknesses, synergism, all medicines share, the deficiency of vital energy can be mended, and blood stasis can fall apart, and wind-phlegm can be dispelled, suppressing the hyperactive liver and subsiding YANG, play benefiting QI for activating blood circulation, the effect of the eliminating phlegm that relieves dizziness, high fever, infantile convulsions, epilepsy, etc., disperse blood stasis and dredge collateral, treating both the principal and secondary aspects of a disease altogether.
5, experimental result confirms that the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) is to rat CCl
4chronic hepatic injury has protective effect.The composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) can reduce serum alt, AST activity; Rising TP, ALB; Rising hepatic tissue SOD, GSH-px activity, reduce Liver MDA; Improving hepatic tissue pathology changes.With strong points, evident in efficacy.
6, the present invention also has the functions such as the blood sugar concentration of reduction, improves body immunity, effective two-ways regulation human immunity state, and internal organs are strong, energetic, and the resist the disease factor prevents harmful pathogenic bacteria infringement human body.
7, raw material is easy to get, the prescription science, and production technology advanced person, workable, moderate product price, be applicable to suitability for industrialized production.
8, the result of product studies on acute toxicity of the present invention and long term toxicity research shows, by intending using product of the present invention the course for the treatment of with daily dose, is safe.
9, Fructus Momordicae has been arranged, has no longer needed correctives, and Fructus Momordicae not only sweet taste is high, can also be as diabetes or do not need crowd's food of sucrose.
The specific embodiment
Embodiment 1
12 parts of Ganodermas; Fructus Lycii 10; 4 parts of Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae)s; 6 parts of Fructus Momordicaes; 30 parts of hydroxypropyl emthylcelluloses; 10 parts of maltodextrins; 1 part of Pulvis Talci.
Its preparation method is following steps:
(1) Ganoderma is cleaned with clear water, be cut into 1~3cm fritter, 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Ganoderma superfine powder, package spare;
(2) Fructus Lycii is cleaned with clear water, 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Fructus Lycii superfine powder, package spare;
(3) Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) is cleaned with clear water, be cut into 1~3cm fritter, 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, package spare;
(4) Fructus Momordicae is cleaned with clear water, be cut into 1~3cm fritter, 100~120 ℃ of hot-air seasonings, are pulverized with super micron mill lower than 5% to moisture, cross 500 mesh sieves, obtain the Fructus Momordicae superfine powder, package spare;
(5) Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, Fructus Momordicae superfine powder, hydroxypropyl emthylcellulose and maltodextrin are proportionally put into V-Mixer, after mix homogeneously, add 60%(V/V under stirring) ethanol, hold agglomerating to material have gentle hands, till loosing one's grip and scattering, make 20 purpose granules with screen cloth, then under 60 ℃, dry moisture content processed is 6%; Be cooled to room temperature, cross 60 mesh sieves, with mix lubricant evenly after, at the 2.5Mpa lower sheeting, obtain the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with tablet machine.
Embodiment 2
10 parts of Ganodermas; Fructus Lycii 15; 5 parts of Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae)s; 8 parts of Fructus Momordicaes; 25 parts of hydroxypropyl emthylcelluloses; 15 parts of maltodextrins; 2 parts of Pulvis Talci.
Its preparation method is:
The preparation technology of Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder and Fructus Momordicae superfine powder is identical with embodiment's 1.
Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, Fructus Momordicae superfine powder, Fructus Momordicae fructoside, hydroxypropyl emthylcellulose and maltodextrin are proportionally put into V-Mixer, after mix homogeneously, add 65%(V/V under stirring) ethanol, hold agglomerating to material have gentle hands, till loosing one's grip and scattering, make 20 purpose granules with screen cloth, then under 70 ℃, dry moisture content processed is 5.6%; Be cooled to room temperature, cross 60 mesh sieves, with mix lubricant evenly after, at the 2.8Mpa lower sheeting, obtain the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with tablet machine.
Embodiment 3
20 parts of Ganodermas; Fructus Lycii 10; 6 parts of Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae)s; 5 parts of Fructus Momordicaes; 20 parts of hydroxypropyl emthylcelluloses; 15 parts of maltodextrins; 2 parts of magnesium stearate.
Its preparation method is:
The preparation technology of Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder and Fructus Momordicae superfine powder is identical with embodiment's 1.
Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, Fructus Momordicae superfine powder, Fructus Momordicae fructoside, hydroxypropyl emthylcellulose and maltodextrin are proportionally put into V-Mixer, after mix homogeneously, add 70%(V/V under stirring) ethanol, hold agglomerating to material have gentle hands, till loosing one's grip and scattering, make 20 purpose granules with screen cloth, then under 75 ℃, dry moisture content processed is 5.2%; Be cooled to room temperature, cross 60 mesh sieves, with mix lubricant evenly after, at the 3.0Mpa lower sheeting, obtain the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with tablet machine.
Embodiment 4
25 parts of Ganodermas; Fructus Lycii 12; 2 parts of Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae)s; 10 parts of Fructus Momordicaes; 24 parts of hydroxypropyl emthylcelluloses; 13 parts of maltodextrins; Magnesium stearate 2.
Its preparation method is:
The preparation technology of Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder and Fructus Momordicae superfine powder is identical with embodiment's 1.
Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, Fructus Momordicae superfine powder, Fructus Momordicae fructoside, hydroxypropyl emthylcellulose and maltodextrin are proportionally put into V-Mixer, after mix homogeneously, add 80%(V/V under stirring) ethanol, hold agglomerating to material have gentle hands, till loosing one's grip and scattering, make 20 purpose granules with screen cloth, then under 70 ℃, dry moisture content processed is 5%; Be cooled to room temperature, cross 60 mesh sieves, with mix lubricant evenly after, at the 3.5Mpa lower sheeting, obtain the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with tablet machine.
Embodiment 5
16 parts of Ganodermas; Fructus Lycii 14; 4 parts of Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae)s; 7 parts of Fructus Momordicaes; 26 parts of hydroxypropyl emthylcelluloses; 12 parts of maltodextrins; 2.5 parts of PEG4000.
Its preparation method is:
The preparation technology of Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder and Fructus Momordicae superfine powder is identical with embodiment's 1.
Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, Fructus Momordicae superfine powder, Fructus Momordicae fructoside, hydroxypropyl emthylcellulose and maltodextrin are proportionally put into V-Mixer, after mix homogeneously, add 85%(V/V under stirring) ethanol, hold agglomerating to material have gentle hands, till loosing one's grip and scattering, make 20 purpose granules with screen cloth, then under 80 ℃, dry moisture content processed is 4%; Be cooled to room temperature, cross 60 mesh sieves, with mix lubricant evenly after, at the 4Mpa lower sheeting, obtain the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with tablet machine.
Embodiment 6
22 parts of Ganodermas; Fructus Lycii 11; 3 parts of Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae)s; 8 parts of Fructus Momordicaes; 28 parts of hydroxypropyl emthylcelluloses; 10 parts of maltodextrins; 1.5 parts of PEG6000.
Its preparation method is:
The preparation technology of Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder and Fructus Momordicae superfine powder is identical with embodiment's 1.
Ganoderma superfine powder, Fructus Lycii superfine powder, Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) superfine powder, Fructus Momordicae superfine powder, Fructus Momordicae fructoside, hydroxypropyl emthylcellulose and maltodextrin are proportionally put into V-Mixer, after mix homogeneously, add 70%(V/V under stirring) ethanol, hold agglomerating to material have gentle hands, till loosing one's grip and scattering, make 20 purpose granules with screen cloth, then under 70 ℃, dry moisture content processed is 5.2%; Be cooled to room temperature, cross 60 mesh sieves, with mix lubricant evenly after, at the 3.2Mpa lower sheeting, obtain the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) with tablet machine.
The composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) of above-mentioned 6 embodiment that prepare has multiple biological activity, find that there is protect the liver, antiinflammatory, raising body immunity and anti-hepatic fibrosis, the effect of anti-apoptotic.The present invention has observed the protective effect of its rat chronic hepatic injury that CCl4 is caused, and its mechanism of action of Primary Study.
1 material
1.1 medicine and reagent:
The composite formula of chewable tablets of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae): inventor embodiment 1-6(Ganoderma; Fructus Lycii; Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae); The Fructus Momordicae water extraction, per kilogram adds water 5-8 kilogram and extracts 2 times, concentrated, removes slag into thick paste, called after LHYL); Bifendate drop pill: Beijing consonance pharmaceutical factory, CCl4(analytical pure): the Shanghai Changjiang River chemical plant produces; ALT, AST measure test kit: bio-engineering research institute is built up in Nanjing; TP, ALB measure test kit: bio-engineering research institute is built up in Nanjing, and SOD, MDA measure test kit: bio-engineering research institute is built up in Nanjing; GSH-px measures test kit: bio-engineering research institute is built up in Nanjing.
1.2 animal:
Clean level Wistar rat, the male and female dual-purpose, body weight 200 ± 20g, Guangxi university Experimental Animal Center provides.
2 methods
2.1 animal grouping and processing:
Rat is divided into 6 groups at random: Normal group, model control group, positive controls, the high, medium and low dosage group of LHYL.Experimental session is except Normal group, and each organizes rat by 3ml/kg (5ml/kg first) body weight back subcutaneous injection 40% CCl4 peanut oil solution, and weekly twice, continuous 8 weeks, cause the rat chronic hepatic injury.Weigh weekly twice, and adjust successively the dosage of medicine and CCl4.Normal group, model control group gavage deionized water; The high, medium and low dosage group of LHYL is gavage LHYL0.8g/kg, 0.4g/kg, 0.2g/kg respectively, positive controls gavage 0.1g/kg bifendate drop pill, and administration two is 10ml/kg.
2.2 sample reception:
After last administration 24h, frog board is rat fixedly, and the femoral artery sacrificed by exsanguination is got the front 12h fasting of blood and be can't help water.Separation of serum, cryopreservation; Get same position hepatic tissue and make 10% homogenate; Getting hepatomegaly leaf 10% formaldehyde fixes.
2.3 index detects:
All detections all detect with U.S. Agilent8543 ultraviolet-uisible spectrophotometer; Detect all and undertaken by the test kit explanation.
2.4 liver histopathology procuratorial work:
Paraffin embedding, section, HE dyeing, observe hepatic tissue pathology and change under light microscopic.By following standard marked [6,7]
(-) 1 minute: the lobules of liver structural integrity, hepatocyte is neat, without abnormal.
(+) 2 minutes: the degeneration of hepatocyte limitation, slight fibrosis, the hepatocyte focal necrosis, have a small amount of cell infiltration.
(++) 3 minutes: the degeneration of hepatocyte diffusivity, necrosis, fibrosis, the lobules of liver structural change, the necrosis of hepatocyte limitation, have more cell infiltration.
(+++) 4 minutes: the degeneration of hepatocyte diffusivity, necrosis, fibrosis is heavier, the heavy damage of lobules of liver structure, a large amount of cell infiltration.
2.5 statistical procedures:
Data with
mean, between two groups, mean difference is checked with t.
3 results
3.1 the impact of LHYL on rat CCl4 chronic hepatic injury serum alt, AST:
CCl4 chronic hepatic injury rat blood serum ALT, AST level obviously raise.Model control group rat blood serum Serum ALT, the horizontal compared with normal matched group of AST obviously raise, P<0.01.With model group, compare, positive controls, four medication groups of the high, medium and low dosage group of LHYL all can significantly reduce CCl4 chronic hepatic injury rat blood serum ALT, AST level, P<0.01 or P<0.05.The results are shown in Table 1
Table 1 LHYL is to rat CCl
4the impact of chronic hepatic injury serum alt, AST
* compare P<0.05 with model control group; * and model control group be P<0.01 relatively
3.2 LHYL is to rat CCl
4the impact of ALB, TP, A/G in chronic hepatic injury serum:
With Normal group, compare, model control group serum ALB, TP content significantly reduce, and the A/G value also significantly reduces, P<0.01.Positive controls serum ALB, TP content, A/G value have remarkable rising effect, P<0.01.With each dosage group of model control group comparison LHYL, ALB, TP content are had to obvious rising effect P<0.01 or P<0.05.The results are shown in Table 2
Table 2 LHYL is to rat CCl
4the impact of ALB, TP, A/G in chronic hepatic injury serum (
, n=12)
* compare P<0.05 with model control group; * and model control group be P<0.01 relatively
3.3 LHYL is to rat CCl
4the impact of chronic hepatic injury liver histopathology:
Model group liver tissues of rats normal configuration is destroyed, and collagen contents increases, and obvious fiber is arranged every formation, liver rope arrangement disorder, and the lobules of liver structure is obviously destroyed.With model group comparison positive controls, each dosage group pathological tissue of LHYL, have clear improvement, in Table 4.
Table 3 LHYL is to CCl
4the pathological impact of chronic hepatic injury liver tissues of rats (
, n=10)
* compare P<0.05 with model group; * and model group be P<0.01 relatively
4. discuss:
We carry out LHYL to rat CCl
4the experimentation of chronic hepatic injury protective effect, experimental result confirms that LHYL is to rat CCl
4chronic hepatic injury has protective effect.LHYL can reduce serum alt, AST activity; Rising TP, ALB; Rising hepatic tissue SOD, GSH-px activity, reduce Liver MDA; Improving hepatic tissue pathology changes.Its mechanism may with Ganoderma; Fructus Lycii; Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae); Lipotropism matter, peroxidating that Fructus Momordicae has are relevant, and concrete mechanism remains further to be studied.
Case 1, Wei * *, the man, 45 years old, the Nanning Enterprise Staff, found abdominal pain in 2013, and bitterly, the soreness of waist, do not feel like eating flatulence all day, and survey report is (TBIL 18.21, and TP 65.26, and ALT 192, and AST 58).Be diagnosed as Type B viral hepatitis, adopt the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) of the embodiment of the present invention 1, every days 3 gram, continuous 30 days, abdominal pain, the symptom of flatulence disappears substantially, then takes medicine 30 days to consolidate curative effect, can go to work at present.
Case 2, deep * *, the female, nineteen fifty-nine gives birth to, the teacher of Liuzhou technical school, in December, 2008 is to hospital outpatient, the readme hepatalgia, the skin light yellow complexion, be diagnosed as Type B viral hepatitis through check (HBV-DNA 3.82 * 104, and TBIL 21.82, and AST 128).Develop the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) of bright embodiment 3, every 1 gram, three times on the one, hepatalgia transference cure after taking medicine 20 days, face starts to occur ruddy.
The composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) of the present invention can also prevent the health preparation of hyperlipidemia, blood sugar lowering, antioxidation, blood fat reducing as middle age, it takes case history:
Case 3, Liu * *, birth in 1966, the civil servant, in the past due to busy all the year round work, have no time to attend to and take good care of one's health so that fall sick from overwork, immunity degradation, feel that health is tired, sit down and just do not remember, that inefficiency, energy are not panted plentifully, upstairs is unable, easy catching a cold, hypomnesis, distraction, face gloomy unglazed, be full of wrinkles, dry rough, the vexed meaning of skin is dry, and suffers from hyperglycemia, hyperlipidemia, easily rush down again, cold perspiration, a large amount of alopecias, poliosis, endure serious illness puzzlement to the fullest extent, lives the painful date.After alleviating a period of time by rest and western medical treatment at that time, the busy old complaint that is not careful in one's diet of work one can recur again, causes sometimes other state of an illness, permanent hospital, have an injection to take medicine and just control table and do not control root, live the low quality life, upon somebody's introduction, take the composite chewable tablet of Ganoderma Corydalis thalictrifolia Franch. (Radix Corydalis Thalictrifoliae) of the present invention, every day the 3-4 sheet, every 1 gram, after 2 months, a skin smooth high resilience that starts to become! Not alopecia, the blackening of poliosis part, sleep improvement, memory improves, and original disease disappears automatically, and whole people is full of youthful vigor, produces huge energy in body, and upper 6 floor ladders do not think tired without a break, have got back to again 10 in vigorous health several years ago the time.