CN103417423B

CN103417423B - Cu, Zn-rhSOD cosmetic composition that a kind of freckle removing and whitening is crease-resistant

Info

Publication number: CN103417423B
Application number: CN201310162935.5A
Authority: CN
Inventors: 刘霞
Original assignee: SHANGHAI YUHUA LIFE TECHNOLOGY DEVELOPMENT Co Ltd
Current assignee: SHANGHAI YUHUA LIFE TECHNOLOGY DEVELOPMENT Co Ltd
Priority date: 2013-05-06
Filing date: 2013-05-06
Publication date: 2015-12-23
Anticipated expiration: 2033-05-06
Also published as: CN103417423A

Abstract

The present invention relates to cosmetic technical field, be specifically related to Cu, Zn-rhSOD cosmetic composition that a kind of freckle removing and whitening is crease-resistant, it comprises emulsifying base, Chinese herbal medicine extract, Percutaneous absorption enhancer, enzyme protective agent alive, Cu, Zn-rhSOD, wherein each constituent mass percentage ratio is as follows: emulsifying base 50-80%, Chinese herbal medicine extract 10-30%, Percutaneous absorption enhancer 1-3%, enzyme protective agent 1-5%, Cu, Zn-rhSOD100-1000U/g alive, and deionized water supplies 100%; Described Chinese herbal medicine extract is the extract of following weight part ratio Chinese crude drug: Stigma Croci 1-5; Radix Salviae Miltiorrhizae 2-8; Radix Glycyrrhizae 5-10; Fructus Phyllanthi 3-6; Poria 2-5, Radix Ampelopsis 1-8, Radix Angelicae Sinensis 1-5.Cosmetic composition of the present invention can remove wrinkle and the mottle of skin appearance; make finer and smoother, smooth, the tender white and springiness of skin; meeting people remove speckle daily skin protection demand to the whitening of skin of face, defying age wrinkle removing, is a kind of superior cosmetics of novel safety.

Description

Cu, Zn-rhSOD cosmetic composition that a kind of freckle removing and whitening is crease-resistant

Technical field

The present invention relates to cosmetic technical field, be specifically related to Cu, Zn-rhSOD cosmetic composition that a kind of freckle removing and whitening is crease-resistant.

Background technology

As everyone knows, due to other factors many in the ultraviolet in sunlight and life, such as computer radiate, smoking is drunk, tension pressure, junk food etc., capital increases the generation of interior free yl, accelerate the oxidation of cell, cause the accelerated ageing of skin, particularly ultraviolet, it is after infiltrating through skin, harmful free radical can be produced, these free radicals can increase the quantity of epithelial lipids peroxide (LPO), destroy collagen protein and the elastic fiber of skin, but also meeting stimulation melanin cell, increase melanic secretion, thus cause xerosis cutis to relax, follow the string, produce wrinkle, flavescence blackening, chromogenesis deposition or freckle etc.

The application of SOD on skin care item mainly comprises in two, and one is as cosmetics additive, can prevent skin aging, play skin effect; Two is the relevant dermatosiss of prevention and therapy.These obtain extensive use abroad.

SOD plays dual function to skin, can promote that collagen protein is formed on the one hand appropriately crosslinked; On the other hand, it can be used as effective free radical scavenger, prevents skin aging, keeps soft smooth.SOD is Absorbable organic halogens collagen protein and elastin laminin also, because of but skin high resilience.

SOD, as one of antioxidant conventional in superior cosmetics, has and significantly reduces wrinkle and effect of dispelling spots.At present, domestic and international many superior cosmetics all with the addition of SOD, and it is made facial film, milk, cream.Domestic have the products such as SOD frost, violet SOD.

Much all add SOD in state's superior cosmetics such as American-European and Japan, main cause has three: one, SOD is special oxygen free radical scavenger; Two, SOD has obvious sun-proof result; Three, SOD has obvious antiphlogistic effects.Carried out extensive research to the toxicity of SOD both at home and abroad, great mass of data shows SOD no side effects, therefore fool proof for cosmetics.But the SOD as organized enzyme is used for as the impact of cosmetic composition by many factors, and as poor heat stability, be very easy to inactivation, the half-life is short; Molecular weight about 32000, not easily through keratodermatitis etc.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of cosmetic composition, not only ensure SOD composition not easy in inactivation, easily transdermal, and wrinkle and the mottle of skin appearance can be removed, promote cutaneous circulation, increase skin-nourishing, make the tenderer white and springiness of skin.

For the above-mentioned technical problem solved; the invention discloses Cu, Zn-rhSOD cosmetic composition that a kind of freckle removing and whitening is crease-resistant; it comprises emulsifying base, Chinese herbal medicine extract, Percutaneous absorption enhancer, enzyme protective agent alive, Cu, Zn-rhSOD, and wherein each constituent mass percentage ratio is as follows:

Emulsifying base 50-80%, Chinese herbal medicine extract 10-30%, Percutaneous absorption enhancer 1-3%, enzyme protective agent 1-5%, Cu, Zn-rhSOD100-1000U/g alive, deionized water supplies 100%; Described Chinese herbal medicine extract is the extract of following weight part ratio Chinese crude drug:

Stigma Croci 1-5; Radix Salviae Miltiorrhizae 2-8; Radix Glycyrrhizae 5-10; Fructus Phyllanthi 3-6; Poria 2-5, Radix Ampelopsis 1-8, Radix Angelicae Sinensis 1-5;

Wherein said Percutaneous absorption enhancer is terpenoid, quintessence oil, one of lactone or ketopyrrolidine apoplexy due to endogenous wind or their combination, be preferably Flos Caryophylli volatile oil, Oleum menthae, Oleum Eucalypti, Borneolum Syntheticum, laurocapram, 4-lauryl alcohol sulfo-good fortune quinoline, N-dodecane-α-pyrrolinone and N-dodecane-2-piperidones to one of in 5-fluorouracil or N-acetyl proline amylalcohol ester or their combination, most preferably be the combination of the Flos Caryophylli volatile oil of weight ratio 1: 1: 1, Oleum menthae and laurocapram.

Wherein, described enzyme protective agent of living is tetrahydropyrimidine, Isosorbide-5-Nitrae, one of in 5,6-tetrahydrochysene-2-methyl 4-pyrimidine carboxylic, vitamin c class polyphenoils or trehalose or their combination, is preferably tetrahydropyrimidine and the combination of vitamin c class polyphenoils of weight ratio 1: 10.

Wherein, described vitamin c class polyphenoils is one of in vitamin C, Magnesium L-Asacorbic Acid 2-O-Phosphate, vitamin C alkyl phosphate, VCE, vitamin C mucopolysaccharide or their combination, is preferably Magnesium L-Asacorbic Acid 2-O-Phosphate or vitamin alkyl phosphate.

Wherein emulsifying base is produce emollient that cosmetics routine uses, wetting agent, emulsifying agent, dispersant, antioxidant, aromatic, antiseptic, collagen protein, chitosan, yeast extract, polysaccharide, carotenoid, a kind of in lecithin or their combination.

Described emulsifying base is Polyoxyethylene Methyl Glucoside Ether-20 sesquistearate 1.5-2.5% of percent mass, octadecanol 5-6%, methyl glucoside sesqui-stearate 2-3%, Ethylhexyl stearate 5-6%, mineral oil 13-15%, methyl hydroxybenzoate 0.2-0.3%, propylparaben 0.1-0.3%, glycerol 10-11%, essence 0.2-0.4% and miaow ureine 0.1-0.3%, and deionized water supplies 100%;

Described SOD is rhSOD-Cu/Zn Cu Zn-SOD (Cu/Zn-rhSOD) or high temperature SOD.

Croceous effect for dredge channels and activating collaterals, stimulating the menstrual flow silt, scattered silt relieving accumulation of pathogens, reducing swelling and alleviating pain, nourish blood enrich blood, promoting the production of body fluid and benefiting QI, removing pathogenic heat from blood and toxic substance from the body; Poria energy dispelling speckles and whitening face, moist skin, can also strengthen immunologic function, blood vessel dilating, can also enhancing human body immunity function; Radix Glycyrrhizae invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription, active ingredient is glycyrrhizin, has and promotes that the storage of sodium, water is stayed and Detoxication; Cortex Fraxini has heat clearing away, removing fire from the liver effect; Radix Salviae Miltiorrhizae has blood circulation promoting and blood stasis dispelling, nourishing blood to tranquillize the mind, effect of removing heat from blood detumescence; Fructus Phyllanthi has clearing away heat and cooling blood, promoting digestion and invigorating the stomach, cough-relieving of promoting the production of body fluid, its effective ingredient emblic element (Emblica), and human body melanocyte and horn cell are cultivated test result and shown there is inhibitory action to B16 cell, have good freckle removing and whitening effect; Radix Angelicae Sinensis has to enrich blood invigorates blood circulation, menstruction regulating and pain relieving, effect of loosening bowel to relieve constipation.

Cosmetic composition of the present invention makes SOD activity-maintaining time longer, more easily infiltrate skin and be less readily available for absorption by the skin, more effectively scavenging free radicals is clear, and the extract of Stigma Croci, Radix Salviae Miltiorrhizae, Radix Glycyrrhizae, Fructus Phyllanthi, Poria, Radix Ampelopsis and Radix Angelicae Sinensis can improve skin blood microcirculation, check melanin synthesis, dispelling speckles and whitening face, skin of moistening simultaneously.Cosmetic composition of the present invention can remove wrinkle and the mottle of skin appearance; make finer and smoother, smooth, the tender white and springiness of skin; can meet people remove speckle daily skin protection requirement to the whitening of skin of face, defying age wrinkle removing easily, be a kind of superior cosmetics of novel safety.

Detailed description of the invention

Below in conjunction with specific embodiment, set forth the present invention further.These embodiments are only not used in for illustration of the present invention and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, the usually conveniently conditioned disjunction condition of advising according to manufacturer.Unless otherwise defined, all specialties used in literary composition and scientific words and one skilled in the art the same meaning be familiar with.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The use that better implementation method described in literary composition and material only present a demonstration.

The preparation of embodiment 1, Chinese herbal medicine extract

Formula (weight part ratio) is as following table 1:

	Extract 1	Extract 2	Extract 3	Extract 4
					Stigma Croci	5	3	1	5
Radix Salviae Miltiorrhizae	3	6	8	2
					Radix Glycyrrhizae	8	5	5	10
Fructus Phyllanthi	4	3	6	5
					Poria	5	3	2	4

Radix Ampelopsis	6	8	1	3
					Radix Angelicae Sinensis	3	5	5	1

The preparation method of Chinese herbal medicine extract (being called for short: extract) is: pulverized respectively by the prepared slices of Chinese crude drugs such as Stigma Croci, Radix Salviae Miltiorrhizae, Radix Glycyrrhizae, Fructus Phyllanthi, Poria, Radix Ampelopsis, Radix Angelicae Sinensis, then carry out 40 orders to sieve, add 8 times contain deionized water ethanol to Chinese medicine weight portion more respectively, its concentration of alcohol is respectively 50%-85%; Then soak more than 10 hours under 25 DEG C of conditions; Carry out low temperature (<80 DEG C) ultrasonic extraction 4 hours, get supernatant centrifugal, filter, refluxing ethanol, after secondary filter, extract 1-4 can be obtained.

The SOD cosmetics preparation that embodiment 2, freckle removing and whitening are crease-resistant

According to 10000g product meter, each constituent content proportioning is as following table 2 (g)

Table 2

	Emulsifying base	Chinese herbal medicine extract	Percutaneous absorption enhancer	Enzyme protective agent alive	Deionized water	SOD
							1	5000	3000	300	100	1600	0.2
2	8000	1000	100	500	198	2
							3	6000	2000	200	500	1300	0.4
4	6000	1000	200	200	2600	0.4
							5	6000	1000	200	200	2600	0.4
6	6000	1000	200	200	2600	0.4
							7	6000	1000	200	200	2600	0.4
8	6000	1000	200	200	2600	0.4

Chinese herbal medicine extract is extract 1-4 prepared by embodiment 1, and being wherein extract 3 in numbering 1 cosmetics, is extract 4 in numbering 2 cosmetics, and being extract 1 in numbering 3 cosmetics, is extract 2 in numbering 4-8 cosmetics.

SOD: rhSOD-Cu/Zn Cu Zn-SOD (Cu/Zn-rhSOD), 5000u/mg Shanghai Yuhua Life Science Technonlogy Development Co., Ltd.; In cosmetics, the work of SOD enzyme is: 1:100u/g; 2:1000u/g; 3::1000u/g; 4-8:200u/g;

Described Percutaneous absorption enhancer: 1-4 is laurocapram; 5 is Flos Caryophylli volatile oil, Oleum menthae and the laurocapram combination that Flos Caryophylli volatile oil, 6 is Oleum menthae, 7-8 is weight ratio 1: 1: 1;

Described enzyme protective agent: the 1-4 that lives is vitamin C, and 5-6 is vitamin C alkyl phosphate, and 7 is tetrahydropyrimidine, 8 is tetrahydropyrimidine and the vitamin C alkyl phosphate of weight ratio 1: 10;

Described emulsifying base is Polyoxyethylene Methyl Glucoside Ether-20 sesquistearate 2.5% of percent mass, octadecanol 5%, methyl glucoside sesqui-stearate 3%, Ethylhexyl stearate 6%, mineral oil 15%, methyl hydroxybenzoate 0.2%, propylparaben 0.2%, glycerol 10%, essence 0.2% and miaow ureine 0.1%, and deionized water supplies 100%;

Preparation method:

1, prepare burden by above-mentioned formula: by glycerol and deionized water mix homogeneously, be designated as A phase; Wherein, water adds 2.5% into total formulation content; Polyoxyethylene Methyl Glucoside Ether-20 sesquistearate (SSE-20), octadecanol, methyl glucoside sesqui-stearate, Ethylhexyl stearate, mineral oil, methyl hydroxybenzoate, propylparaben Hybrid Heating are melted, is designated as B phase;

2, sterilizing: A, B are heated to 92 DEG C respectively, maintains sterilizing in 18 minutes;

3, emulsifying: to be added to by B in A phase and constantly to stir, keeping temperature about 82 DEG C, homogenizer 1550rpm rotating speed down cut 8 minutes; Emulsifying began to cool down after 22 minutes, was cooled to 39 DEG C, added SOD lyophilized powder+enzyme protective agent mixture alive, Chinese herbal medicine extract, Percutaneous absorption enhancer, essence, the mixing of miaow ureine;

5, cooling packing: detect through conventional cosmetics quality and safe and sanitary, qualified rear packaging.

The long-term Enzyme activity assay of SOD in embodiment 3, cosmetics

By the cosmetics numbering 3-8 (SOD inventory is 200U/g) prepared by embodiment 2, assay NBT photoreduction (Zhang Hong is adopted in different time points, Tan Zhujun " University of the Inner Mongol's journal: natural science edition " the 6th phase 677-681 page in 2002) detect the work of SOD enzyme, result is as table 3.

Table 3

Embodiment 4, Transdermal Absorption are tested

Cosmetics numbering 1-8 (SOD inventory is 200u/g) prepared by Example 2 is appropriate, simultaneously with according to embodiment 2 numbering 8 formula, remove Percutaneous absorption enhancer, prepare cosmetics product in contrast, be spread evenly across on the Corii Sus domestica of Franz diffusion cell, get receiving liquid after 12h appropriate, detect SOD enzyme and live.Found that reference substance SOD enzyme is lived as 8U/ml, cosmetics numbering 2 sample SOD enzyme is lived as 15U/ml, cosmetics numbering 1,3-6 sample SOD enzyme are lived all not high, are about 10U/ml, and cosmetics numbering 7-8 sample SOD enzyme is lived as 28U/ml, 32U/ml.

The crease-resistant test of embodiment 5, freckle removing and whitening

After person on probation washes one's face every day sooner or later, smear the cosmetics prepared by embodiment 2, on probation after 2 weeks, assessment result, evaluation criteria: skin physiology imbalance disappears or partial disappearance is effective; Do not disappear for invalid.

Cosmetics numbering 8 (SOD200u/g) prepared by embodiment 2

Type	Test total number of persons	Efficiently individual quantity	Invalid number	Effective percentage
					Freckle removing and whitening	20	18	2	90％
Crease-resistant	35	32	3	91％
					Comedo	50	42	8	84％

Cosmetics numbering 2 (SOD1000u/g) prepared by embodiment 2

Type	Test total number of persons	Efficiently individual quantity	Invalid number	Effective percentage
					Freckle removing and whitening	20	15	5	75％
Crease-resistant	35	30	5	86％
					Comedo	50	40	10	80％

Scope of the present invention is not by the restriction of described specific embodiments, and described embodiment, only as the single example of illustrating various aspects of the present invention, also comprises method and the component of functional equivalent in the scope of the invention.In fact, except content as herein described, those skilled in the art can easily grasp multiple improvement of the present invention with reference to description above and accompanying drawing.Described improvement also falls within the scope of appended claims.Every section of list of references mentioned above is listed in herein as a reference all in full.

Claims

1. Cu, Zn-rhSOD cosmetic composition that freckle removing and whitening is crease-resistant, it comprises emulsifying base, Chinese herbal medicine extract, Percutaneous absorption enhancer, enzyme protective agent alive, Cu, Zn-rhSOD, and wherein each constituent mass percentage ratio is as follows:

Described enzyme protective agent alive is tetrahydropyrimidine and the vitamin C alkyl phosphate of weight ratio 1: 10.

2. Cu, Zn-rhSOD cosmetic composition as claimed in claim 1, is characterized in that described Percutaneous absorption enhancer is Flos Caryophylli volatile oil, Oleum menthae, Oleum Eucalypti, Borneolum Syntheticum, laurocapram, 4-lauryl alcohol sulfo-good fortune quinoline, N-dodecane-α-pyrrolinone and N-dodecane-2-piperidones to one of in 5-fluorouracil or N-acetyl proline amylalcohol ester or their combination.

3. Cu, Zn-rhSOD cosmetic composition as claimed in claim 2, is characterized in that described Percutaneous absorption enhancer is the Flos Caryophylli volatile oil of weight ratio 1: 1: 1, Oleum menthae and laurocapram combination.

4. Cu, Zn-rhSOD cosmetic composition as claimed in claim 1, it is characterized in that described emulsifying base is Polyoxyethylene Methyl Glucoside Ether-20 sesquistearate 1.5-2.5% of mass percent, octadecanol 5-6%, methyl glucoside sesqui-stearate 2-3%, Ethylhexyl stearate 5-6%, mineral oil 13-15%, methyl hydroxybenzoate 0.2-0.3%, propylparaben 0.1-0.3%, glycerol 10-11%, essence 0.2-0.4% and miaow ureine 0.1-0.3%, deionized water supplies 100%.

5. Cu, Zn-rhSOD cosmetic composition as claimed in claim 1, is characterized in that described Cu, Zn-rhSOD are rhSOD-Cu/Zn Cu Zn-SOD.

6. Cu, Zn-rhSOD cosmetic composition as claimed in claim 1, is characterized in that it is made up of following ingredients mass percent:

Emulsifying base 60%, Chinese herbal medicine extract 10%, Percutaneous absorption enhancer 2%, enzyme protective agent 2% alive, Cu, Zn-rhSOD200U/g, deionized water supplies 100%; Described Chinese herbal medicine extract is the extract of following weight part ratio Chinese crude drug:

Stigma Croci 3; Radix Salviae Miltiorrhizae 6; Radix Glycyrrhizae 5; Fructus Phyllanthi 3; Poria 3; Radix Ampelopsis 8; Radix Angelicae Sinensis 5;

Described Percutaneous absorption enhancer is the combination of the Flos Caryophylli volatile oil of weight ratio 1: 1: 1, Oleum menthae and laurocapram;

Described enzyme protective agent alive is tetrahydropyrimidine and the vitamin C alkyl phosphate of weight ratio 1: 10;

Described emulsifying base is Polyoxyethylene Methyl Glucoside Ether-20 sesquistearate 2.5% of percent mass, octadecanol 5%, methyl glucoside sesqui-stearate 3%, Ethylhexyl stearate 6%, mineral oil 15%, methyl hydroxybenzoate 0.2%, propylparaben 0.2%, glycerol 10%, essence 0.2% and miaow ureine 0.1%, and deionized water supplies 100% composition.