CN103393121A - Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions - Google Patents
Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions Download PDFInfo
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a preparation method of an enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions. The method includes: taking malt, rice sprouts, hawthorns, dried tangerine peel, finger citron, coix seeds, white hyacinth beans, Gorgon fruits, radish seeds, sharpleaf galangal fruits and the like, adding water to conduct soaking, and performing extraction, subjecting the extracted solution to conventional thermal reflux compulsory circulation, adding brown sugar and peptone, carrying out blending, sterilizing and filtering, thus obtaining the filtrate, i.e. a nutrient solution; inoculating probiotics into the nutrient solution at 30-32DEG C, performing mixed fermentation in four stages, and conducting quality detection, thus obtaining a drink. The method provided in the invention adopts a plurality of plants to serve as a nutrient solution culture medium, takes a compound probiotic as a conversion tool, and employs multistage liquid submerged fermentation. Toxic and harmful matters in the raw materials can be degraded, new active compounds can be formed, protease and glucoamylase are produced during fermentation, so that the flavor of the original material product can be improved. The phytic acid in plants is converted into functional lactic acid and acetic acid, and insoluble fiber is converted into soluble dietary fiber and the like. The prepared product can activate a variety of enzymes in the human body and comprehensively regulate human body metabolism.
Description
Technical field
The present invention relates to a kind of gastrointestinal function that improves, contain the preparation method of enzyme compound probiotic plant enzyme.
Background technology
" stomach " that the traditional Chinese medical science refers in fact comprises the upper gastrointestinal region of anatomy and the physiological functions such as oesophagus, Stomach duodenum.Traditional Chinese medicine is having its unique advantage aspect treatment stomach trouble.According to the principle of diagnosis and treatment, take the method for " cold person's temperature it, treating heat with cold drug, treatment of deficiency-syndrome by reinforcement, treating excess syndrome with purgative methods ", adjusting patient's physique, and according to the principle of " general rule is not bitterly " to regulate the flow of vital energy, to invigorate blood circulation and stomach, pain relieving, reach the purpose for the treatment of.Common saying is said " stomach trouble three is divided and ruled, supported in seven minutes ", and that as seen takes good care of is important.Traditional Chinese medical science nourishing the stomach is that the different physique, Various Seasonal according to the people, the different stomach trouble of suffering from are carried out dialectical taking good care of, and controls to reach prevention of diseae, ill morning, the purpose of quick recovery, minimizing recurrence.
The saying that ten people's nine stomach trouble are just arranged from ancient times.Stomach trouble is a kind of very general defect, reasonable diet is one of essential condition of nourishing the stomach, China treats in ancient times stomach trouble and mostly adopts the plant treatment, and nourishing the stomach plant commonly used has Poria cocos, the bighead atractylodes rhizome, the Radix Astragali, ginseng, Huai Shan, the seed of Job's tears, glossy ganoderma, sealwort, wilsonii, the root of straight ladybell, date, Radix Glycyrrhizae etc.
Many new methods and product are more arranged now; as " a kind of stomach strengthening and digestion promoting electuary " (Chinese patent application number 97109362); that the Chinese herbal medicines such as appropriate coloured malt, rice sprout, seed of Job's tears, Radix Codonopsis, Chinese yam, Poria cocos, lotus seeds, the membrane of a chicken's gizzard, reed rhizome are made cream clearly together with being placed in after decoction → filtration → evaporation and concentration; clear cream is closed in machine and mixes the companion with sucrose, with making after drying, screening after the granulator granulation.the Baokang polynary probiotic oral liquid of spirit and preparation method as " the polynary probiotic oral liquid of Baokang spirit and the preparation method for the treatment of disease of digestive system " (Chinese patent application number 201210232084.2) treatment disease of digestive system, by Radix Codonopsis, the bighead atractylodes rhizome, the Radix Astragali, Chinese yam, Fructus Crataegi, Fructus Hordei Germinatus, Radix Glycyrrhizae, Poria cocos, glossy ganoderma, dried orange peel, Buddha's hand, fructus amomi, the bark of official magnolia, agalloch eaglewood, the rhizome of nutgrass flatsedge, matrimony vine, Radix Angelicae Sinensis, the red sage root, the bletilla striata, extra large octopus shells, radix bupleuri, the root of herbaceous peony, prolong recklessly, radix glehniae, radix polygonati officinalis, the fruit of Chinese magnoliavine, coptis root, the peeling Semen Raphani, Semen Lablab Album, Radix Notoginseng powder, the dried immature fruit of citron orange, the banksia rose, coix seed, the membrane of a chicken's gizzard, fructus corni, lotus rice, balcony is decocted soup and is formed." a kind of traditional Chinese medicine oral liquid for the treatment of the liver and gall enterogastric diseases " (Chinese patent application number 201210504757.5), have Radix Codonopsis, the bighead atractylodes rhizome, the Radix Astragali, Chinese yam, Fructus Crataegi, Fructus Hordei Germinatus, dried orange peel, Radix Angelicae Sinensis, the red sage root, the bletilla striata, the root of herbaceous peony, the membrane of a chicken's gizzard, Radix Glycyrrhizae, Poria cocos, Buddha's hand, fructus amomi, the bark of official magnolia, agalloch eaglewood, the rhizome of nutgrass flatsedge, matrimony vine, the cuttlebone of shelling, radix bupleuri, radix glehniae, radix polygonati officinalis, the fruit of Chinese magnoliavine, peeling radish seed, Semen Lablab Album, the dried immature fruit of citron orange, the banksia rose, coix seed, the root of three-nerved spicebush.
Ferment, be actually the old of enzyme and translate.Enzyme is the large biological molecule with biocatalytic Activity, i.e. biocatalyst, and it can accelerate the speed of biochemical reaction, but does not change direction and the product of reaction.State after ferment is decomposed by peptic digest with food is identical, is the power that helps peptic digest, and has anti-inflammatory, antibacterial action, can promote self-healing ability and the power of regeneration of human body.The food that absorbs in the middle of daily life has starch, fat, protein etc. macromolecular structure, and these foods can not directly utilize, and ferment is to play the part of the effect that the food cutting is decomposed.In life, the ferment total amount that can produce voluntarily in body is certain to the people, and human body is along with the variation at age, and the ferment in body can lack or defect, therefore supplementary necessity is arranged.
Research is pointed out: the Cucumber of Chinese medicine may have facilitation to the generation of microbial growth metabolism and active component, and microorganism is decomposed the noxious material in Chinese medicine, can detoxify and reduce its toxic and side effect.
Summary of the invention
The objective of the invention is for above-mentioned present situation, aiming to provide a kind ofly can increase the quantity of probio in human body intestinal canal, and good mouthfeel, but long-term taking are taken the gastrointestinal function that improves of safety non-toxic, contain the preparation method of enzyme compound probiotic plant enzyme.
The embodiment of the object of the invention is, a kind of gastrointestinal function that improves contains the preparation method of enzyme compound probiotic plant enzyme, and concrete steps are as follows:
1) clean raw material,
2) raw material extracts, get raw material and be placed in extractor, add water to 10 times of raw material weight more than amount, raw material all is immersed in water, heating water temperature to 60 ℃, soak 6-10 hour, be heated to again more than 100 ℃, extract after 30-50 minute, with extracting liquid filtering out, in the 1:8 ratio again extracting in water once, extracted twice liquid merges;
described raw material is: Fructus Hordei Germinatus 20-30 gram, rice sprout 20-30 gram, Hericium erinaceus 20-30 gram, hawthorn 10-20 gram, Chinese yam 20-30 gram, dried orange peel 10-20 gram, Buddha's hand 10-15 gram, fructus amomi 10-12 gram, coix seed 20-30 gram, Poria cocos 10-15 gram, matrimony vine 10-20 gram, cape jasmine 5-10 gram, radix polygonati officinalis 10-15 gram, purslane 20-30 gram, Semen Lablab Album 20-30 gram, Gorgon fruit 20-30 gram, Radix Glycyrrhizae 5-10 gram, lotus seeds 10-12 gram, sealwort 10-30 gram, radish seed 6-10 gram, the membrane of a chicken's gizzard 8-10 gram, fructus alpiniae oxyphyllae 3-10 gram,
3) with step 2) merging extract normal temperature, normal pressure, hot reflux force to follow bad, be concentrated into proportion 1.06-1.08;
4) with the concentrate of step 3), add 5-8% brown sugar, 2% peptone blending, 105 ℃ of high-temperature sterilizations 40 minutes, filter, and filtrate is nutrient solution;
5) cooling, inoculation, be cooled to 30-32 ℃ with the nutrient solution of step 4) in fermentation tank, connects the compound probiotic kind, mixed culture fermentation,
Described compound probiotic kind is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and acetobacter;
Described bacterial classification inoculum concentration is the 5%-10% of nutrient solution;
6) fermentation, the fermentation minute fourth phase,
The first phase, the oxygen supply yeast phase, oxygen supply 250-300L/min, the fermentation tank pressure-controlled, at 0.07～0.08MPa, keeps room temperature 22-25 ℃, fermentation temperature 30-32 ℃, time 3-7 days,
The second phase, acetobacter participates in acid process, fermentation temperature 30-32 ℃, and time 5-7 days,
The third phase, the lactobacter growth animated period, micro-aerobe fermentation, 1.2L/min, 37 ℃ of fermentation temperatures, time 5-7 days,
The fourth phase, 28 ℃-30 ℃ of fermentation temperatures, observed seven days, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state;
7) quality detecting index is packaged into finished product after requiring detection qualified according to product standard.
the present invention cultivates base take various plants as nutrient solution, take the multiple compound probiotic of tradition as the conversion instrument, in the plant that can cause due to environmental pollution, the venomous injurants such as pollutant and agricultural chemicals are degraded, to form new active component, can make effective ingredients in plant and multiple probio play best synergy. can take full advantage of and improve in raw material the release to the favourable functional component of health, can ferment phytic acid in plant to become functional lactic acid and acetic acid by profound level under the effect of probio phytase, in plant, insoluble fibre becomes soluble dietary fiber, improve the effect of original plant.Multiple compound bacteria is utilized the catalytic action of enzyme in various plants, the product that produces after fermenting by profound level can human activin in the effect of multiple biology enzyme.The plant enzyme of the present invention's preparation is the balance the body metabolic function comprehensively.
The specific embodiment
The selected nutrient solution of the present invention is scientific and reasonable, Chinese yam protection stomach lining, promotion digestion; Poria cocos: the spleen-stomach diseases is controlled in the appetizing of regulating the flow of vital energy; Buddha's hand protection stomach lining, promotion digestion; Dried orange peel has the digestion of promotion, protection stomach lining, defaecation; Hawthorn promotes the effects such as digestion, food digesting stomach fortifying; The radix polygonati officinalis nourishing the stomach to improve the production of body fluid, stomach is mended in the lotus seeds invigorating the spleen, and the membrane of a chicken's gizzard promotes digestion.Food digesting stomach fortifying; Matrimony vine is quenched one's thirst and draws drink, and Radix Glycyrrhizae is weakness of the spleen and the stomach, coordinating the drug actions of a prescription.The radish seed de-swelling that helps digestion, relax bowel, promote digestion; Job's tears heart of a lotus seed benevolence strengthens immunity, invigorating the spleen; The Fructus Hordei Germinatus promote qi circulation digestion promoting, spleen benefiting and stimulating the appetite; Fructus Hordei Germinatus and rice sprout spleen benefiting and stimulating the appetite, help digestion.Hericium erinaceus is nutritious, improves a poor appetite, and strengthens the barrier of gastric mucosa function.The dried orange peel appetizing of regulating the flow of vital energy.Fructus amomi dampness elimination appetizing, warming spleen and stopping diarrha.The cape jasmine gastric acid inhibitory.The purslane detoxifcation, Semen Lablab Album invigorating the spleen, Gorgon fruit tonifying spleen stopping leak, sealwort benefit temper; Fructus alpiniae oxyphyllae cures mainly deficiency-cold in spleen and stomach; Vomiting.
Full side is used for weakness of the spleen and the stomach, taste make and, abdominal fullness and distention, anorexia and loose stool.Replenish qi to invigorate the spleen, poor appetite.To healthy population or the daily long-term drinking of sub-health population also have invigorating the spleen, nourishing the stomach, protect liver, relax stomach and
Concrete steps of the present invention are as follows:
1) clean raw material, reject substandard products, poor products, not genuine kind; In order to ensure product quality,, with the qualified quality raw materials that clean,, according to the different purposes of product,, according to the Chinese medicine preparation standard, adopt distinct methods to make with extra care, fling to volatile oil as dried orange peel, the method for removing is that the low baking temperature frying got final product in 5-10 minute.
Raw material mainly contains: alkaloids, flavonoid glycosides, polysaccharide etc., but this class material only contains in certain this plant, and content is extremely low.Most of below 5%, only a few content reaches minimumly below 10% has 1,000,000/; Two classes are auxiliary compositions, and adjunct ingredient is to have less important physiologically active and health-care effect, and wherein some auxiliary composition has practical value equally, mainly be to provide probio and raw material supplements the nutrients in fermentation, three classes are invalid components, and utilization rate is low,, for utility is proposed, need to adopt extraction process.
2) raw material extracts, and gets raw material and is placed in extractor, adds water to 10 times of raw material weight more than amount, and raw material all is immersed in water, and heating water temperature to 60 ℃, soak 3-10 hour, best 6 hours; Be heated to again more than 100 ℃, decoct after 30-50 minute, decoction liquor is filtered out, in the 1:8 ratio again boiling once, twice decoction liquor merges.
described raw material is: Fructus Hordei Germinatus 20-30 gram, rice sprout 20-30 gram, Hericium erinaceus 20-30 gram, hawthorn 10-20 gram, Chinese yam 20-30 gram, dried orange peel 10-20 gram, Buddha's hand 10-15 gram, fructus amomi 10-12 gram, coix seed 20-30 gram, Poria cocos 10-15 gram, matrimony vine 10-20 gram, cape jasmine 5-10 gram, radix polygonati officinalis 10-15 gram, purslane 20-30 gram, Semen Lablab Album 20-30 gram, Gorgon fruit 20-30 gram, Radix Glycyrrhizae 5-10 gram, lotus seeds 10-12 gram, sealwort 10-30 gram, radish seed 6-10 gram, the membrane of a chicken's gizzard 8-10 gram, fructus alpiniae oxyphyllae 3-10 gram.
3) with step 2) merging extract normal temperature, normal pressure, conventional hot reflux force to follow bad, be concentrated into proportion 1.06-1.08.
4) with the concentrate of step 3), add 5-8% brown sugar, 2% peptone nutrition article blending, 105 ℃ of high-temperature sterilizations 40 minutes, filter, and gets filtrate and be nutrient solution;
5) cooling, inoculation, be cooled to 30-32 ℃ with the nutrient solution of step 4) in fermentation tank, connects the compound probiotic kind, mixed culture fermentation,
Described compound probiotic kind is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and acetobacter.
Inoculation method is: bacterial classification utilizes the direct automatic transport of pipeline to fermentation tank from culturing room, and the bacterial classification inoculum concentration is the 5-10% of nutrient solution.
6) fermentation, the fermentation minute fourth phase,
The first phase, the oxygen supply yeast phase, oxygen supply 250-300L/min, while generally fermenting, tall can pressure-controlled is at 0.07～0.08MPa.Generally the highest tank pressure is that the fermentation maximum temperature value is divided by the 100(units MPa).Adopt fermentation with pressure, can suppress the propagation of yeast, reduce the too much phenomenon of metabolic by-product that causes owing to heating up, prevent the generation of excessive higher alcohol, ester class, keep room temperature 22-25 ℃, fermentation temperature 30-32 ℃, time 3-7 days.
The second phase, acetobacter participates in acid process, fermentation temperature 30-32 ℃, time 5-7 days.
The third phase, the lactobacter growth animated period, micro-aerobe fermentation, 1.2L/min, fermentation temperature transfer to 37 ℃, time 5-7 days.
The fourth phase, fermentation temperature transfers to 28 ℃-32 ℃, observes seven days, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state.
7) quality testing, pH value 3-4, edible fungus (saccharomycete) ml＞1 * 10
6Individual, the every ml of lactic acid bacteria＞1 * 10
6Individual, acetobacter is differentiated the dull and stereotyped transparent circle that produces according to calcium carbonate, Hygienic Index: plumbous (in pb) ㎎/L＜0.5, arsenic (in As) ㎎/L＜0.3, Ge ㎎/L＜0.2, Gong ㎎/L＜0.3 etc., coliform, MPN/10ml＜0.3, pathogenic bacteria (duodenum 12 road pathogenic bacteria and pathogenic coccus) must not detect.Soluble solid 0.5%, the main component of nutrient solution: Zong Huang Tong ㎎/100g 〉=60, the indexs such as soluble dietary fiber (㎎/100ml) 〉=500.
The improve gastrointestinal function of Hubei Province disease prevention and cure control centre to the present invention's preparation, contain enzyme compound probiotic plant enzyme and done zoopery.
test situation is as follows: by 120 milliliters/60kg of people's daily intaking amount BW, enlarge respectively 10, 20, 30 times as the intestinal motility test dose, enlarge 5, 10, 20 times (namely 10, 20, 40 milliliters/kgBW) as peptic digest enzyme test dosage, adopt SPF level Kunming mouse and Wistar rat, gave continuously tested material 30 days, result of the test shows: with the control group ratio, each dosage group all can obviously increase pepsin activity, obviously increase per hour endoproteinase discharge rate, small intestine mechanical movement and model control group comparing difference to mouse have conspicuousness (P＜0.05, 0.01) estimate the criterion regulation according to health food: tested material has the effect of the digestive function of promotion.
Below enumerate specific embodiments of the invention.
Example 1,
1) clean raw material, dried orange peel low baking temperature frying 5 minutes, remove volatile oil.
2) raw material extracts, raw material after cleaning, after processing is placed in extractor, add water to 10 times of raw material weight more than amount, raw material all is immersed in water, heating water temperature to 60 ℃, soaked 6 hours, be heated to again 100 ℃, decoct after 50 minutes, decoction liquor is filtered out, in the 1:8 ratio again boiling once, twice decoction liquor merges;
Described raw material is: Fructus Hordei Germinatus 20 grams, rice sprout 30 grams, Hericium erinaceus 20 grams, hawthorn 10 grams, Chinese yam 20 grams, dried orange peel 10 grams, Buddha's hand 10 grams, fructus amomi 10 grams, coix seed 20 grams, Poria cocos 15 grams, matrimony vine 10 grams, cape jasmine 6 grams, radix polygonati officinalis 12 grams, purslane 22 grams, Semen Lablab Album 30 grams, Gorgon fruit 22 grams, Radix Glycyrrhizae 5 grams, lotus seeds 10 grams, sealwort 30 grams, radish seed 6 grams, the membrane of a chicken's gizzard 8 grams, fructus alpiniae oxyphyllae 3 grams;
3) with step 2) merging extract normal temperature, normal pressure, conventional hot reflux force to follow bad, be concentrated into proportion 1.06,
4) with the concentrate of step 3), add 5% brown sugar, 2% peptone blending, 105 ℃ of high-temperature sterilizations 40 minutes, filter, and gets filtrate and be nutrient solution;
5) cooling, inoculation, the nutrient solution of step 4) is cooled to 30 ℃ in fermentation tank, from culturing room utilize pipeline directly with saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and the direct automatic transport of acetobacter bacterial classification to fermentation tank, the bacterial classification inoculum concentration is 5% of nutrient solution.
6) fermentation, the fermentation minute fourth phase,
The first phase, oxygen supply yeast phase, oxygen supply 250L/min, tank pressure 0.08MPa during fermentation.Adopt fermentation with pressure, can suppress the propagation of yeast, reduce the too much phenomenon of metabolic by-product that causes owing to heating up, prevent the generation of excessive higher alcohol, ester class, keep 22 ℃ of room temperatures, 32 ℃ of fermentation temperatures, 3 days time.
The second phase, acetobacter participates in acid process, 30 ℃ of fermentation temperatures, 6 days time.
The third phase, the lactobacter growth animated period, micro-aerobe fermentation, 1.2L/min, fermentation temperature transfer to 37 ℃, 5 days time.
The fourth phase, fermentation temperature transfers to 28 ℃, observes seven days, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state.
7) quality testing, pH value 3-4, edible fungus (saccharomycete) ml＞1 * 10
6Individual, the every ml of lactic acid bacteria＞1 * 10
6Individual, acetobacter is differentiated the dull and stereotyped transparent circle that produces according to calcium carbonate, Hygienic Index: plumbous (in pb) ㎎/L＜0.5, arsenic (in As) ㎎/L＜0.3, Ge ㎎/L＜0.2, Gong ㎎/L＜0.3 etc., coliform, MPN/10ml＜0.3, pathogenic bacteria (duodenum 12 road pathogenic bacteria and pathogenic coccus) must not detect.Soluble solid 0.5%, the main component of nutrient solution: Zong Huang Tong ㎎/100g 〉=60, the indexs such as soluble dietary fiber (㎎/100ml) 〉=500.Detect qualified rear packing.
Example 2, with example 1, different is,
1) frying of dried orange peel low baking temperature is 7 minutes, removes volatile oil.
2) raw material extracts, and heating raw materials water temperature to 60 ℃, soaked 8 hours, then be heated to 100 ℃, decocts after 40 minutes, and decoction liquor is filtered out;
Described raw material is: Fructus Hordei Germinatus 25 grams, rice sprout 22 grams, Hericium erinaceus 30 grams, hawthorn 15 grams, Chinese yam 24 grams, dried orange peel 13 grams, Buddha's hand 11 grams, fructus amomi 10 grams, coix seed 22 grams, Poria cocos 11 grams, matrimony vine 14 grams, cape jasmine 5 grams, radix polygonati officinalis 13 grams, purslane 27 grams, Semen Lablab Album 24 grams, Gorgon fruit 20 grams, Radix Glycyrrhizae 6 grams, lotus seeds 10 grams, sealwort 10 grams, radish seed 7 grams, the membrane of a chicken's gizzard 8 grams, fructus alpiniae oxyphyllae 5 grams;
3) extract normal temperature, normal pressure, conventional hot reflux are forced to follow bad, are concentrated into proportion 1.07,
4), with the concentrate of step 3), add 6% brown sugar, 2% peptone;
5) cooling, inoculation, the nutrient solution of step 4) is cooled to 32 ℃ in fermentation tank, from culturing room utilize pipeline directly with saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and the direct automatic transport of acetobacter bacterial classification to fermentation tank, the bacterial classification inoculum concentration is 8% of nutrient solution.
6) fermentation, the fermentation minute fourth phase,
The first phase, oxygen supply yeast phase, oxygen supply 260L/min, tank pressure 0.07MPa during fermentation.Adopt fermentation with pressure, can suppress the propagation of yeast, reduce the too much phenomenon of metabolic by-product that causes owing to heating up, prevent the generation of excessive higher alcohol, ester class, keep 23 ℃ of room temperatures, 30 ℃ of fermentation temperatures, 7 days time.
The second phase, acetobacter participates in acid process, 32 ℃ of fermentation temperatures, 5 days time.
The third phase, lactobacter growth animated period, 37 ℃ of fermentation temperatures, fermentation time 6 days.
The fourth phase, fermentation temperature transfers to 29 ℃, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state.
Example 3, with example 1, different is,
1) frying of dried orange peel low baking temperature is 8 minutes, removes volatile oil.
2) raw material extracts, and heating raw materials water temperature to 60 ℃, soaked 8 hours, then be heated to 103 ℃, decocts after 40 minutes, and decoction liquor is filtered out;
Described raw material is: Fructus Hordei Germinatus 30 grams, rice sprout 26 grams, Hericium erinaceus 28 grams, hawthorn 17 grams, Chinese yam 26 grams, dried orange peel 14 grams, Buddha's hand 12 grams, fructus amomi 12 grams, coix seed 30 grams, Poria cocos 14 grams, matrimony vine 20 grams, cape jasmine 5 grams, radix polygonati officinalis 15 grams, purslane 30 grams, Semen Lablab Album 26 grams, Gorgon fruit 30 grams, Radix Glycyrrhizae 9 grams, lotus seeds 12 grams, sealwort 18 grams, radish seed 10 grams, the membrane of a chicken's gizzard 10 grams, fructus alpiniae oxyphyllae 9 grams;
3) extract normal temperature, normal pressure, hot reflux are forced to follow bad, are concentrated into proportion 1.07,
4), with the concentrate of step 3), add 8% brown sugar, 2% peptone;
5) cooling, inoculation, with the nutrient solution of step 4) in fermentation tank, be cooled to 30 ℃, from culturing room utilize pipeline directly with saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and the direct automatic transport of acetobacter bacterial classification to fermentation tank, the bacterial classification inoculum concentration is 7% of nutrient solution.
6) fermentation, the fermentation minute fourth phase,
The first phase, oxygen supply yeast phase, oxygen supply 280L/min, tank pressure 0.07MPa during fermentation.Adopt fermentation with pressure, can suppress the propagation of yeast, reduce the too much phenomenon of metabolic by-product that causes owing to heating up, prevent the generation of excessive higher alcohol, ester class, keep 25 ℃ of room temperatures, 31 ℃ of fermentation temperatures, 7 days time.
The second phase, acetobacter participates in acid process, 32 ℃ of fermentation temperatures, 6 days time.
The third phase, lactobacter growth animated period, 37 ℃ of fermentation temperatures, fermentation time 7 days.
The fourth phase, fermentation temperature transfers to 30 ℃, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state.
Example 4, with example 1, different is,
1) frying of dried orange peel low baking temperature is 10 minutes, removes volatile oil.
2) raw material extracts, and heating raw materials water temperature to 60 ℃, soaked 10 hours, then be heated to 100 ℃, decocts after 30 minutes, and decoction liquor is filtered out;
Described raw material is: Fructus Hordei Germinatus 26 grams, rice sprout 20 grams, Hericium erinaceus 23 grams, hawthorn 20 grams, Chinese yam 30 grams, dried orange peel 20 grams, Buddha's hand 15 grams, fructus amomi 12 grams, coix seed 26 grams, Poria cocos 10 grams, matrimony vine 17 grams, cape jasmine 10 grams, radix polygonati officinalis 10 grams, purslane 20 grams, Semen Lablab Album 20 grams, Gorgon fruit 26 grams, Radix Glycyrrhizae 10 grams, lotus seeds 11 grams, sealwort 28 grams, radish seed 8 grams, the membrane of a chicken's gizzard 9 grams, fructus alpiniae oxyphyllae 10 grams;
3) extract normal temperature, normal pressure, hot reflux are forced to follow bad, are concentrated into proportion 1.08,
4), with the concentrate of step 3), add 7% brown sugar, 2% peptone;
5) cooling, inoculation, with the nutrient solution of step 4) in fermentation tank, be cooled to 30 ℃, from culturing room utilize pipeline directly with saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and the direct automatic transport of acetobacter bacterial classification to fermentation tank, the bacterial classification inoculum concentration is 8% of nutrient solution.
6) fermentation, the fermentation minute fourth phase,
The first phase, oxygen supply yeast phase, oxygen supply 300L/min, tank pressure 0.08MPa during fermentation.Adopt fermentation with pressure, can suppress the propagation of yeast, reduce the too much phenomenon of metabolic by-product that causes owing to heating up, prevent the generation of excessive higher alcohol, ester class, keep 24 ℃ of room temperatures, 31 ℃ of fermentation temperatures, 4 days time.
The second phase, acetobacter participates in acid process, fermentation temperature 30-32 ℃, 7 days time.
The third phase, lactobacter growth animated period, 37 ℃ of fermentation temperatures, fermentation time 6 days.
The fourth phase, fermentation temperature transfers to 30 ℃, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state.
Claims (3)
1. one kind is improved gastrointestinal function, contains the preparation method of enzyme compound probiotic plant enzyme, it is characterized in that concrete steps are as follows:
1) clean raw material,
2) raw material extracts, get raw material and be placed in extractor, add water to 10 times of raw material weight more than amount, raw material all is immersed in water, heating water temperature to 60 ℃, soak 6-10 hour, be heated to again more than 100 ℃, extract after 30-50 minute, with extracting liquid filtering out, in the 1:8 ratio again extracting in water once, extracted twice liquid merges;
described raw material is: Fructus Hordei Germinatus 20-30 gram, rice sprout 20-30 gram, Hericium erinaceus 20-30 gram, hawthorn 10-20 gram, Chinese yam 20-30 gram, dried orange peel 10-20 gram, Buddha's hand 10-15 gram, fructus amomi 10-12 gram, coix seed 20-30 gram, Poria cocos 10-15 gram, matrimony vine 10-20 gram, cape jasmine 5-10 gram, radix polygonati officinalis 10-15 gram, purslane 20-30 gram, Semen Lablab Album 20-30 gram, Gorgon fruit 20-30 gram, Radix Glycyrrhizae 5-10 gram, lotus seeds 10-12 gram, sealwort 10-30 gram, radish seed 6-10 gram, the membrane of a chicken's gizzard 8-10 gram, fructus alpiniae oxyphyllae 3-10 gram,
3) with step 2) merging extract normal temperature, normal pressure, hot reflux force to follow bad, be concentrated into proportion 1.06-1.08;
4) with the concentrate of step 3), add 5-8% brown sugar, 2% peptone blending, 105 ℃ of high-temperature sterilizations 40 minutes, filter, and filtrate is nutrient solution;
5) cooling, inoculation, be cooled to 30-32 ℃ with the nutrient solution of step 4) in fermentation tank, connects the compound probiotic kind, mixed culture fermentation,
Described compound probiotic kind is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and acetobacter;
Described bacterial classification inoculum concentration is the 5%-10% of nutrient solution;
6) fermentation, the fermentation minute fourth phase,
The first phase, the oxygen supply yeast phase, oxygen supply 250-300L/min, the fermentation tank pressure-controlled, at 0.07～0.08MPa, keeps room temperature 22-25 ℃, fermentation temperature 30-32 ℃, time 3-7 days,
The second phase, acetobacter participates in acid process, fermentation temperature 30-32 ℃, and time 5-7 days,
The third phase, the lactobacter growth animated period, micro-aerobe fermentation, 1.2L/min, 37 ℃ of fermentation temperatures, time 5-7 days,
The fourth phase, 28 ℃-30 ℃ of fermentation temperatures, observed seven days, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state;
7) quality detecting index is packaged into finished product after requiring detection qualified according to product standard.
2. a kind of gastrointestinal function that improves according to claim 1, contain the preparation method of enzyme compound probiotic plant enzyme, it is characterized in that cleaning raw material, and dried orange peel is removed volatile oil, and the method for removing is low baking temperature frying 5-10 minute.
3. a kind of gastrointestinal function that improves according to claim 1, contain the preparation method of enzyme compound probiotic plant enzyme, it is characterized in that raw material all is immersed in water, and heating water temperature to 60 ℃, soaked 6 hours.
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