CN103876144A - Preparation method of probiotic-containing compound plant enzyme - Google Patents
Preparation method of probiotic-containing compound plant enzyme Download PDFInfo
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- CN103876144A CN103876144A CN201410128795.4A CN201410128795A CN103876144A CN 103876144 A CN103876144 A CN 103876144A CN 201410128795 A CN201410128795 A CN 201410128795A CN 103876144 A CN103876144 A CN 103876144A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a probiotic-containing compound plant enzyme. The probiotic-containing compound plant enzyme is prepared by selecting various vegetables and medicinal and edible plants as fermentation substrates and adopting various probiotics/yeasts as fermentation inoculums through multi-period discontinuous fed-batch fermentation and multi-stage deep fermentation. The probiotic-containing compound plant enzyme prepared through the preparation method has the advantages of various types of contained enzymes, high activity, good stability and small influence from environment. By adopting the method, different substrates can be fermented by using different bacteria in different time periods, so that different active ingredients contained in various fermentation substrates can be more completely extracted and the fermentation of the whole system can be more sufficient and harmonious.
Description
Technical field
The present invention relates to technical field of biological fermentation, particularly a kind of preparation method of the composite plant ferment containing profitable probliotics.
Background technology
China's enzyme preparation industry, through the development of more than 50 years, has entered big country's ranks that world's enzyme preparation is produced.The enzyme preparation of having accomplished scale production at present reaches 30 kinds of left and right; but major part is that single enzyme preparation is take carbohydrase, amylase, phytase, cellulase, protease, pectase etc. as main; wherein carbohydrase, amylase and phytase output are crossed ten thousand tons; main application fields concentrates on glucose industry, brewing industry, dairy processing industry, baking food, fuel alcohol industry, wine and beverage industry, detergent industry, paper industry etc., aspect food applications also in the operational phase as food additives.Its active ingredient is single, and effect is also not obvious.
Enzyme is the large biological molecule with biocatalytic Activity, i.e. biocatalyst, and it can accelerate the speed of biochemical reaction, but does not change direction and the product of reaction.But different from other abiotic catalyst, enzyme has the selectivity of height, and a catalysis is specifically reacted or produced specific configuration.Thereby single enzyme composition is single, can only a certain large biological molecule of catalysis, and be not suitable for diversified large biological molecule substrate in living nature, old friends conduct in-depth research complex enzyme, and its development is more and more paid close attention to.
And existing common complex enzyme mostly is feed complex enzyme and beer complex enzyme, it mixes by single enzyme or through the fruits and vegetables extraction of naturally continuously fermenting, the complex enzyme activity that mixing or fruits and vegetables mix the rear gained of extraction is simultaneously low, and active constituent content is low, main component only limits to limited several enzymes, and large, poor stability affected by environment, its main cause be different strain growth conditions and fermentating metabolism product difference very large.Become inevitable developing direction therefore develop a kind of material that contains good stability and active ingredient complex enzyme how.
Summary of the invention
For the problems referred to above of prior art existence, the invention discloses a kind of preparation method of the composite plant ferment containing profitable probliotics.
The preparation method who the present invention relates to a kind of composite plant ferment (also containing the plant fermentation thing of composite enzyme) containing profitable probliotics, the method comprises the following steps:
(1) raw material choose: select fruit, vegetables, cereal, beans, medicine food dual purpose plant and available mushroom and as fermentation substrate; Select moral gram yeast, complete formula yeast, streptococcus thermophilus, bacillus amyloliquefaciens as fermentation inoculum;
(2) processing of fermentation inoculum: under suitable condition, each bacterial classification is carried out to multistage fermentation, expands and cultivate, stop fermentation in the time reaching exponential phase, obtain each strain fermentating liquid, respectively as the fermentation inoculum of handling well;
(3) one grade fermemtation: each strain fermentating liquid of the step of choosing any one kind of them (2) gained is as fermentation inoculum, the strain fermentating liquid of selection is seeded on the fermentation substrate of selected one or more mixing, carry out solid fermentation one time, obtain one grade fermemtation thing;
(4) multistage fermentation: repeating step (3), carries out solid fermentation n time, until obtain the required composite plant ferment containing profitable probliotics; Wherein, using before in fermentation the fermentation substrate described in original step (1) and the upper level fermentate obtaining that ferments mix the fermentation substrate used as every grade, in fermenting before, untapped strain fermentating liquid is as every grade of inoculum used, n is 1,2,3,4;
Wherein, in step (2), (3), (4), in solid fermentation system, the proportioning of the inoculum concentration of fermentation, mixed culture fermentation substrate and fermentation time and temperature are determined according to experiment.
Further, in described step (3), inoculum concentration is 3%～10% (mass percent), and fermentation temperature is 15 ℃～45 ℃, and fermentation time is 10～60 days, obtains one grade fermemtation thing.
Further, in described step (4), inoculum concentration is 3%～10% (mass percent), and fermentation temperature is 15 ℃～45 ℃, and fermentation time is 1～20 day.
Further, in described step (4) in the time that n is 3, described preparation method carries out level Four fermentation altogether, the described plant that fermentations at different levels are selected is sequentially followed successively by cereal and beans, vegetables and available mushroom, medicine food dual purpose plant, fruit, and the described strain fermentating liquid order that fermentations at different levels are selected is followed successively by moral gram yeast, finishes formula yeast, streptococcus thermophilus, bacillus amyloliquefaciens.
Further, in described step (1), described fruit is apple, pears, orange, hawthorn, grape, red date, strawberry, mulberries, sweet orange, cherry, lemon, banana, pawpaw, mango, Kiwi berry, sugarcane, carambola, persimmon or guava.
Further, in described step (1), described vegetables are spinach, bean sprouts, green pepper, celery, cabbage, pumpkin, bottle gourd, onion, broccoli, lotus rhizome, Chinese cabbage, ternip, tomato or carrot.
Further, in described step (1), described cereal is brown rice, corn, millet, black rice, buckwheat, oat, seed of Job's tears or sorghum rice.
Further, in described step (1), described beans is soybean, broad bean, pea, mung bean, soya bean, red bean, red bean, black soya bean, kidney bean seed, black beans, Semen Phaseoli Vulgaris, Kidney bean, broad bean, cowpea or jequirity.
Further, in described step (1), described medicine food dual purpose plant is Radix Glycyrrhizae, cassia bark, American Ginseng, matrimony vine, chrysanthemum, honeysuckle, tawny daylily, FI puerariae, mulberry leaf, sweet osmanthus, aloe or rose.
Further, in described step (1), described available mushroom is asparagus, Hericium erinaceus, mushroom, flat mushroom, pleurotus eryngii, auricularia auriculajudae or white fungus.
Further, the described composite plant ferment containing profitable probliotics comprises amylase, lipase, protease, cellulase, 1,4 beta-glucanase, zytase and lactase.
Further, described amylase activity is 0～290U/g (amylase activity is not 0), described lipase active >=30U/g, described proteinase activity >=5U/g, described cellulase activity >=5U/g, described 1,4 beta-glucanase activity >=5U/g, described xylanase activity 5U/g and described lactase activity >=5U/g.
The preparation method of a kind of composite plant ferment containing profitable probliotics of the present invention, according to different strain growth conditions and and the very large feature of different plant metabolites difference, adopt discontinuous fed-batch fermentation of multicycle, using various plants as fermentation substrate, and using multiple probio/yeast as fermentation inoculum, after multistage submerged fermentation, prepare the plant fermentation thing that is rich in plurality of enzymes.The kind of the enzyme that prepared plant fermentation thing contains is many, active high, and good stability is affected by environment little.Use this method different time sections to use the different strain different plants of fermenting, make the contained different extracts active ingredients of all kinds of plants more complete, whole system fermentation is more fully coordinated.
The specific embodiment
Below in conjunction with the specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Described method is conventional method if no special instructions, and the raw material that use and detection agents useful for same all can obtain from open commercial sources if no special instructions.
Wherein, in the product making, the activity of enzyme classes is measured by following method:
Protease: QB/T1803-1993
Amylase activity: GB8275-2009
Cellulase: QB2583-2003
Lipase: QB/T23535-2009
1,4 beta-glucanase: NY/T911-2004
Zytase: GB/T23874-2009
Lactase: U.S. food chemistry code the 5th edition (FCC V)
Moral gram yeast, complete formula yeast, streptococcus thermophilus and 4 kinds of bacterial classifications of bacillus amyloliquefaciens can expand cultivation by prior art, obtain required moral gram yeast, finish formula yeast, streptococcus thermophilus and bacillus amyloliquefaciens zymotic fluid as the use of fermentation inoculum.
Embodiment 1
Step 1: one grade fermemtation: get black rice, rice, millet, mung bean and black soya bean 5 equal portions (quality) fermentation substrate as one grade fermemtation, carry out after ozonization processing, under cleaning condition, mix, add moral gram yeast fermentation broth as fermentation inoculum in the ratio of inoculum concentration 5wt%, fermentation 20-40 days, fermentation temperature is controlled at 40 ℃-45 ℃, carries out one grade fermemtation and obtains one grade fermemtation thing.
Step 2: second order fermentation: mix after getting auricularia auriculajudae, white fungus, onion, broccoli and celery 5 equal portions (quality) ozonization, as vegetables and the available mushroom fermentation substrate of second order fermentation, mix as second order fermentation substrate according to the mass ratio of 1: 1 with the one grade fermemtation thing obtaining after one grade fermemtation in step 1, add and finish formula yeast fermentation broth as fermentation inoculum in the ratio of inoculum concentration 5wt%, fermentation 5-10 days, fermentation temperature is controlled at 32 ℃-37 ℃, carries out second order fermentation and obtains second order fermentation thing.
Step 3: three grade fermemtation: evenly mix after getting matrimony vine, honeysuckle, FI puerariae, mulberry leaf and sweet osmanthus 5 equal portions (quality) ozonization, as the medicine food dual purpose plant fermentation substrate of three grade fermemtation, mix as three grade fermemtation substrate according to the mass ratio of 1: 2 with the second order fermentation thing obtaining after second order fermentation in step 2, add streptococcus thermophilus fermentation liquid as fermentation inoculum in the ratio of inoculum concentration 7wt%, fermentation 5-10 days, fermentation temperature is controlled at 32 ℃-37 ℃, carries out three grade fermemtation and obtains three grade fermemtation thing.
Step 4: level Four fermentation: evenly mix after getting apple, pears, hawthorn, lemon and orange 5 equal portions (quality) ozonization, as the fruit fermentation substrate of level Four fermentation, mix as level Four fermentation substrate according to the mass ratio of 1: 3 with the three grade fermemtation thing obtaining after three grade fermemtation in step 3, add bacillus amyloliquefaciens zymotic fluid as fermentation inoculum in the ratio of inoculum concentration 5wt%, fermentation 5-10 days, fermentation temperature is controlled at 28 ℃, carries out level Four fermentation and obtains final composite plant ferment.
After testing, in the composite plant ferment finally obtaining, contain 7 kinds of enzymes, wherein amylase activity is 265U/g, lipase active 78U/g, proteinase activity 14U/g, cellulase activity 22U/g, 1,4 beta-glucanase activity 15U/g, xylanase activity 5U/g and lactase activity 8U/g.
Composite plant ferment containing profitable probliotics prepared by the present embodiment can be degraded into simpler structure by the molecular structure of organic substance rapidly, be digested to final inert substance through microorganism, realize decomposing the decomposition of various compounds, organic matter, toxin, be usually used in relieving the effect of alcohol, protect the products such as liver, whitening, reparation skin injury.
Embodiment 2
Step 1: one grade fermemtation: get black rice, rice and mung bean 3 equal portions (quality) fermentation substrate as one grade fermemtation, carry out after ozonization processing, under cleaning condition, mix, add moral gram yeast fermentation broth as fermentation inoculum in the ratio of inoculum concentration 7wt%, ferment 20 days, fermentation temperature is controlled at 40 ℃, carries out one grade fermemtation and obtains one grade fermemtation thing.
Step 2: second order fermentation: mix after getting auricularia auriculajudae and white fungus 2 equal portions (quality) ozonization, as the available mushroom fermentation substrate of second order fermentation, mix as second order fermentation substrate according to the mass ratio of 2: 3 with the one grade fermemtation thing obtaining after one grade fermemtation in step 1, add and finish formula yeast fermentation broth as fermentation inoculum in the ratio of inoculum concentration 5wt%, ferment 2 days, fermentation temperature is controlled at 35 ℃, carries out second order fermentation and obtains second order fermentation thing.
Step 3: three grade fermemtation: evenly mix after getting sweet osmanthus and rose 2 equal portions (quality) ozonization, as the medicine food dual purpose plant fermentation substrate of three grade fermemtation, mix as three grade fermemtation substrate according to the mass ratio of 2: 5 with the second order fermentation thing obtaining after second order fermentation in step 2, add streptococcus thermophilus fermentation liquid as fermentation inoculum in the ratio of inoculum concentration 7wt%, ferment 5 days, fermentation temperature is controlled at 32 ℃, carries out three grade fermemtation and obtains three grade fermemtation thing.
Step 4: level Four fermentation: evenly mix after getting lemon, strawberry and sweet orange 3 equal portions (quality) ozonization, as the fruit fermentation substrate of level Four fermentation, mix as level Four fermentation substrate according to the mass ratio of 3: 7 with the three grade fermemtation thing obtaining after three grade fermemtation in step 3, add bacillus amyloliquefaciens zymotic fluid as fermentation inoculum in the ratio of inoculum concentration 5wt%, ferment 2 days, fermentation temperature is controlled at 28 ℃, carries out level Four fermentation and obtains final composite plant ferment.
After testing, in the composite plant ferment finally obtaining, contain 7 kinds of enzymes, wherein amylase activity is 282U/g, lipase active 55U/g, proteinase activity 8U/g, cellulase activity 10U/g, 1,4 beta-glucanase activity 15U/g, xylanase activity 5U/g and lactase activity 6U/g.
Composite plant ferment containing profitable probliotics prepared by the present embodiment can be degraded into simpler structure by the molecular structure of organic substance rapidly, be digested to final inert substance through microorganism, realize decomposing the decomposition of various compounds, organic matter, toxin, be usually used in whitening, repair the products such as skin injury.
Application Example 3
Relieving alcoholism and protecting liver food---salubrious chewable tablets: get the composite plant ferment, rice protein powder, soyabean protein powder, glucose, the food additives that obtain in above-described embodiment 1 37%: 30%: 30% in mass ratio: measure mixing at 2%: 1%, make the salubrious chewable tablets with liver protection effect.Wherein food additives can be selected one or more in xylitol, acesulfame potassium, citric acid, flavoring essence, ascorbic acid (vitamin C), peppermint, 1-lysine, L-Methionine and betaine as required.
Eating method: with before the meal or drink before and after, chew 1 to 2 better; Main Function fresh breath, relieving alcoholism and protecting liver.
The test of relieving the effect of alcohol: 63 volunteer (42 male sex, 21 women) drink 2 tinned beers (330ml) simultaneously, volunteer is divided into 3 groups: first group is blank group (14 male sex, 7 women), does not take any food that relieves the effect of alcohol after drinks beer; Second group be drinks beer after, the volunteer (14 male sex, 7 women) who takes 4 GSHs (Wakie) capsule; The 3rd group be drinks beer after, the volunteer (14 male sex, 7 women) who takes two complex enzyme sheets; Then by breath alcohol analyzer 10,20,30 and 40 minutes horizontal checkout alcohol contents after drinking.
The alcohol content that the each group of table 1 volunteer tests after 10,20,30 and 40 minutes after drinking
As can be seen from Table 1, blank group of time test in 40 minutes and test in the time of 10 minutes after drinking, in the gas of characteristics of contaminated respiratory droplets, alcohol content increases on the contrary; And after drinking after edible GSH, test and test in the time of 10 minutes 40 minutes time after drinking, in the gas of characteristics of contaminated respiratory droplets, alcohol content reduces 1.59%; After drinking after the salubrious chewable tablets of edible the present embodiment, test and test in the time of 10 minutes 40 minutes time after drinking, in the gas of characteristics of contaminated respiratory droplets, alcohol content reduces 69.35%, and successful relieves the effect of alcohol.
Application Example 4
Household chemicals---toxin expelling perfumed soap: get the composite plant ferment, tartaric acid, vitamin E, rice bran extract, camomile extract of gained in above-described embodiment 2 and rose ethereal oil extract 51%: 2%: 2% in mass ratio: measure at 35%: 5%: 5% and mix as the effective adding ingredient of perfumed soap, make toxin expelling perfumed soap according to conventional perfumed soap method for making.
This toxin expelling perfumed soap does not conform to bleaching agent, chemical dye, not containing essence, is suitable for any skin quality; It contains efficient protection face and body skin composition, especially greasy, prickly heat, fash and the eczema of removing face, back is had to remarkable result, and can solve the overworked problem of skin trace.This toxin expelling perfumed soap can effectively be removed various bacteriums and the aged cells on skin equally, keeps the tender of regeneration skin.The characteristic that biology enzyme perfumed soap is showing is most remove at once the various stinks of health and can prevent mosquito bite and insect bite.
Above-mentioned example is just for illustrating the present invention, and embodiments of the present invention are not limited to these examples, and what those skilled in the art made meets the various specific embodiment of inventive concept all within protection scope of the present invention.
Claims (10)
1. a preparation method who contains the composite plant ferment of profitable probliotics, is characterized in that, said method comprising the steps of:
(1) raw material choose: select fruit, vegetables, cereal, beans, medicine food dual purpose plant and available mushroom and as fermentation substrate; Select moral gram yeast, complete formula yeast, streptococcus thermophilus, bacillus amyloliquefaciens as fermentation inoculum;
(2) processing of fermentation inoculum: under suitable condition, each bacterial classification is carried out to multistage fermentation, expands and cultivate, stop fermentation in the time reaching exponential phase, obtain each strain fermentating liquid, respectively as the fermentation inoculum of handling well;
(3) one grade fermemtation: each strain fermentating liquid of the step of choosing any one kind of them (2) gained, as fermentation inoculum, is seeded to the strain fermentating liquid of selection on the fermentation substrate of selected one or more mixing, carries out solid fermentation one time, obtains one grade fermemtation thing;
(4) multistage fermentation: repeating step (3), carries out solid fermentation n time, until obtain the required composite plant ferment containing profitable probliotics; Wherein, using before in fermentation the fermentation substrate described in original step (1) and the upper level fermentate obtaining that ferments mix the fermentation substrate used as every grade, in fermenting before, untapped strain fermentating liquid is as every grade of inoculum used, n is 1,2,3,4;
Wherein, in step (2), (3), (4), in solid fermentation system, the proportioning of the inoculum concentration of fermentation, mixed culture fermentation substrate and fermentation time and temperature are determined according to experiment.
2. preparation method as claimed in claim 1, is characterized in that, in described step (3), inoculum concentration is 3% ~ 10%(mass percent), fermentation temperature is 15 ℃ ~ 45 ℃, fermentation time is 10 ~ 60 days, obtains one grade fermemtation thing.
3. preparation method as claimed in claim 1, is characterized in that, in described step (4), inoculum concentration is 3% ~ 10%(mass percent), fermentation temperature is 15 ℃ ~ 45 ℃, fermentation time is 1 ~ 20 day.
4. preparation method as claimed in claim 1, it is characterized in that, in described step (4) in the time that n is 3, described preparation method carries out level Four fermentation altogether, the described plant that fermentations at different levels are selected is sequentially followed successively by cereal and beans, vegetables and available mushroom, medicine food dual purpose plant, fruit, and the described strain fermentating liquid order that fermentations at different levels are selected is followed successively by moral gram yeast, finishes formula yeast, streptococcus thermophilus, bacillus amyloliquefaciens.
5. preparation method as claimed in claim 1, is characterized in that, the described composite plant ferment containing profitable probliotics comprises amylase, lipase, protease, cellulase, 1,4 beta-glucanase, zytase and lactase.
6. preparation method as claimed in claim 5, it is characterized in that, described amylase activity is that 0 ~ 290U/g(amylase activity is not 0), described lipase active >=30U/g, described proteinase activity >=5U/g, described cellulase activity >=5U/g, described 1,4 beta-glucanase activity >=5U/g, described xylanase activity >=5U/g and described lactase activity >=5U/g.
7. the preparation method as described in as arbitrary in claim 1 ~ 6, it is characterized in that, in described step (1), described fruit is apple, pears, orange, hawthorn, grape, red date, strawberry, mulberries, sweet orange, cherry, lemon, banana, pawpaw, mango, Kiwi berry, sugarcane, carambola, persimmon or guava.
8. the preparation method as described in as arbitrary in claim 1 ~ 6, it is characterized in that, in described step (1), described vegetables are spinach, bean sprouts, green pepper, celery, cabbage, pumpkin, bottle gourd, onion, broccoli, lotus rhizome, Chinese cabbage, ternip, tomato or carrot; Described available mushroom is asparagus, Hericium erinaceus, mushroom, flat mushroom, pleurotus eryngii, auricularia auriculajudae or white fungus.
9. the preparation method as described in as arbitrary in claim 1 ~ 6, is characterized in that, in described step (1), described cereal is brown rice, corn, millet, black rice, buckwheat, oat, seed of Job's tears or sorghum rice; Described beans is soybean, broad bean, pea, mung bean, soya bean, red bean, red bean, black soya bean, kidney bean seed, black beans, Semen Phaseoli Vulgaris, Kidney bean, broad bean, cowpea or jequirity.
10. the preparation method as described in as arbitrary in claim 1 ~ 6, is characterized in that, in described step (1), described medicine food dual purpose plant is Radix Glycyrrhizae, cassia bark, American Ginseng, matrimony vine, chrysanthemum, honeysuckle, tawny daylily, FI puerariae, mulberry leaf, sweet osmanthus, aloe or rose.
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| CN110452943A (en) * | 2019-08-26 | 2019-11-15 | 厦门市民安创享实业有限公司 | A kind of novel plant starch biology coagulating agent and preparation method thereof |
| CN111227146A (en) * | 2020-03-16 | 2020-06-05 | 重庆市梅灵丹生物科技有限公司 | Fruit and vegetable enzyme source liquid, preparation method and scavenger drink |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102266054A (en) * | 2011-07-26 | 2011-12-07 | 西藏月王生物技术有限公司 | Aweto ferment and preparation method thereof |
| CN102940288A (en) * | 2012-11-19 | 2013-02-27 | 商丘市饮之健食品有限公司 | Composite enzyme beverage and preparation process method thereof |
| CN103393121A (en) * | 2013-06-17 | 2013-11-20 | 湖北富程祥云生物科技有限公司 | Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions |
-
2014
- 2014-04-01 CN CN201410128795.4A patent/CN103876144A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102266054A (en) * | 2011-07-26 | 2011-12-07 | 西藏月王生物技术有限公司 | Aweto ferment and preparation method thereof |
| CN102940288A (en) * | 2012-11-19 | 2013-02-27 | 商丘市饮之健食品有限公司 | Composite enzyme beverage and preparation process method thereof |
| CN103393121A (en) * | 2013-06-17 | 2013-11-20 | 湖北富程祥云生物科技有限公司 | Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions |
Non-Patent Citations (1)
| Title |
|---|
| 毛建卫等: "微生物酵素研究进展", 《发酵科技通讯》, vol. 39, no. 3, 31 July 2010 (2010-07-31), pages 42 - 44 * |
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