CN103382231A - Method for extracting hyaluronic acid from jellyfish - Google Patents

Abstract

The invention discloses a method for extracting hyaluronic acid from jellyfish. The method comprises the steps of raw material proposal, soaking, extraction, alcohol precipitation and drying. The hyaluronic acid obtained by means of the method is characterized in that the hyaluronic acid is white amorphous powder, is odorless, tasteless and easily absorbs moisture, and the content is higher than 94%.

Description

Extract hyaluronic method from jellyfish

Technical field

The present invention relates to a kind of hyaluronic preparation method, be specifically related to a kind of hyaluronic method of extracting from jellyfish.

Background technology

Jellyfish is a kind of large-scale edible jellyfish, has widely in China to distribute, and has very high economic worth and nutritive value.According to surveying and determination, every hectogram jellyfish contains protein 12.3 grams, carbohydrate 4 grams, 182 milligrams of calcium, iodine 132 micrograms and multivitamin and collagen protein.Collagen protein contains hydrophilic natural moisturizing factor, and the powerful pinning moisture of triple-helix structure energy, perviousness to skin is strong, can see through stratum corneum and be combined with the skin epithelial cell, participate in and improve the metabolism of skin cells, make active reinforcement of collagen protein in skin, it can keep the integrity of stratum corneum moisture and fibrous texture, improve the metabolism of skin cells living environment and promotion skin histology, strengthen blood circulation, reach the purpose of skin care.The gloss of skin depends on water content, and the water retention capacity that collagen protein is good makes the skin beauty water profit glossy, distributes healthy brilliance.After collagen protein is absorbed by the skin, be filled between dermis of skin, increase tight skin density, produce skin tension, dwindle pore, make the tight and high resilience of skin.Collagen layer plentiful in corium is propped up skin cells, in conjunction with the effect of moisturizing and inhibition wrinkle, jointly reaches the effect of unfolding coarse groove, desalination microgroove.Active collagen itself has anti-inflammatory and upgrades the effect of skin, can directly infiltrate skin and be used for filling damaged (as the comedo scar) that dark wrinkle, skin injury cause and the defective of the repairing shape of face etc.

The jellyfish body contains Moisture factor, the Typical Representative thing hyaluronic acid (HA) of Moisture factor, HA is the Moisture factor of superior performance recognized in the world, carboxyl in its molecule and other polarity genes can form hydrogen bond with water and in conjunction with a large amount of water, when content lower than 0.1% the time, HA can be in conjunction with the water of 1000 times of its quality, this natural moisturizing factor can make moisture content of skin increase, horny layer of epidermis is soft, improve the blood circulation of skin surface, strengthen skin elasticity, stop the epidermic cell fibrosis, thereby play skin care effect.But HA and protein are to exist with bonding state, are difficult for extracting and purifying.Along with the raising day by day of people's living standard, increasing people more pays attention to healthy and beauty treatment, and the cosmetics that the pure natural extraction is made will more be favored, and the market outlook potentiality are very large.

Summary of the invention

The invention provides a kind of hyaluronic method of extraction take jellyfish as raw material that technique is simple, efficient is high.

The present invention is achieved through the following technical solutions:

A kind ofly extract hyaluronic method take jellyfish as raw material, comprise the steps:

A. raw material is processed: get fresh jellyfish, shred, dry at shady and cool place;

B. soak: get the jellyfish after drying, add the acetone of 2 weight parts to soak 24 hours, until the jellyfish hardening;

C. extract: the jellyfish of step in taking-up, add 3-5 weight part 0.5M acetic acid heating and refluxing extraction 6-8h, filter, collect filtrate, (chloroform, propyl carbinol mixed solution (chloroform: propyl carbinol=4:1)) stir, and be standing to add isopyknic Seavg reagent in the filtrate;

D. alcohol precipitation: get supernatant liquor and add 2 weight part 95%(v/v after standing demix) ethanol stirs, and standingly makes layering, filters;

E. get filtrate, repeat the d step once;

F. dry: with the filtrate that obtains after upper step ethanol precipitation twice, carry out vacuum-drying, vacuum tightness maintains 0.1MPa, minute two stage dryings, fs keeps 85 ℃ of temperature, and the time kept 1 hour, then heats up, 115 ℃ of subordinate phase temperature, the time kept 3 hours, got product.

The hyaluronic acid that adopts method of the present invention to obtain has following proterties: white amorphous powder, and odorless, tasteless, easily moisture absorption, content is more than 94%.

Adopt method of the present invention to extract hyaluronic acid take jellyfish as raw material, compared with prior art, advantage of the present invention:

1, soak jellyfish with acetone and can make the jellyfish dehydration;

2, adopt the mode of reflux to extract purifying, compare direct heating and extract, effect is fine;

3, add in filtrate Seavg reagent (chloroform: propyl carbinol=4:1) can effectively remove protein, then after standing demix except Deproteinization impurity and other colloids;

4, the vacuum-drying vacuum keep is at 0.1MPa, and in minute two stages, different temperature, time are dry, and the products obtained therefrom proterties is kept for some time drying than same temperature, and effect will be got well.

Embodiment

The invention will be further described with embodiment for the below, but the present invention is not limited to these embodiment.

Embodiment 1:

A kind ofly extract hyaluronic method take jellyfish as raw material, comprise the steps:

A. raw material is processed: get fresh jellyfish, shred, dry at shady and cool place;

B. soak: get the jellyfish after drying, add the acetone of 2 weight parts to soak 24 hours, until the jellyfish hardening;

C. extract: the jellyfish of step in taking-up, add 3 weight part 0.5M acetic acid heating and refluxing extraction 8h, filter, collect filtrate, (chloroform, propyl carbinol mixed solution (chloroform: propyl carbinol=4:1)) stir, and be standing to add isopyknic Seavg reagent in the filtrate;

D. alcohol precipitation: get supernatant liquor and add 2 weight part 95%(v/v after standing demix) ethanol stirs, and standingly makes layering, filters;

E. get filtrate, repeat the d step once;

F. dry: with the filtrate that obtains after upper step ethanol precipitation twice, carry out vacuum-drying, vacuum tightness maintains 0.1MPa, minute two stage dryings, and the fs keeps 85 ℃ of temperature, time kept 1 hour, then heat up, 115 ℃ of subordinate phase temperature, the time kept 3 hours, get product, content 94.5%.

Embodiment 2:

A kind ofly extract hyaluronic method take jellyfish as raw material, comprise the steps:

A. raw material is processed: get fresh jellyfish, shred, dry at shady and cool place;

B. soak: get the jellyfish after drying, add the acetone of 2 weight parts to soak 24 hours, until the jellyfish hardening;

C. extract: the jellyfish of step in taking-up, add 5 weight part 0.5M acetic acid heating and refluxing extraction 6h, filter, collect filtrate, (chloroform, propyl carbinol mixed solution (chloroform: propyl carbinol=4:1)) stir, and be standing to add isopyknic Seavg reagent in the filtrate;

D. alcohol precipitation: get supernatant liquor and add 2 weight part 95%(v/v after standing demix) ethanol stirs, and standingly makes layering, filters;

E. get filtrate, repeat the d step once;

F. dry: with the filtrate that obtains after upper step ethanol precipitation twice, carry out vacuum-drying, vacuum tightness maintains 0.1MPa, minute two stage dryings, and the fs keeps 85 ℃ of temperature, time kept 1 hour, then heat up, 115 ℃ of subordinate phase temperature, the time kept 3 hours, get product, content 95.5%.

Embodiment 3:

A kind ofly extract hyaluronic method take jellyfish as raw material, comprise the steps:

A. raw material is processed: get fresh jellyfish, shred, dry at shady and cool place;

B. soak: get the jellyfish after drying, add the acetone of 2 weight parts to soak 24 hours, until the jellyfish hardening;

C. extract: the jellyfish of step in taking-up, add 4 weight part 0.5M acetic acid heating and refluxing extraction 8h, filter, collect filtrate, (chloroform, propyl carbinol mixed solution (chloroform: propyl carbinol=4:1)) stir, and be standing to add isopyknic Seavg reagent in the filtrate;

D. alcohol precipitation: get supernatant liquor and add 2 weight part 95%(v/v after standing demix) ethanol stirs, and standingly makes layering, filters;

E. get filtrate, repeat the d step once;

F. dry: with the filtrate that obtains after upper step ethanol precipitation twice, carry out vacuum-drying, vacuum tightness maintains 0.1MPa, minute two stage dryings, and the fs keeps 85 ℃ of temperature, time kept 1 hour, then heat up, 115 ℃ of subordinate phase temperature, the time kept 3 hours, get product, content 96.3%.

Embodiment 4:

With the resulting product of embodiment 1, identify with the infrared spectrophotometer method.

Detecting instrument: infrared spectrometer;

Wavelength region: 4000～400cm ^-1, 64 times/s of sweep velocity, pellet technique is adopted in sample preparation.

Operate by the infrared spectrophotometry working method, the collection of illustrative plates contrast of the collection of illustrative plates that scans and standard HA, retention time unanimously gets final product.

Claims (1)

1. one kind is extracted hyaluronic method take jellyfish as raw material, comprises the steps:

A. raw material is processed: get fresh jellyfish, shred, dry at shady and cool place;

B. soak: get the jellyfish after drying, add the acetone of 2 weight parts to soak 24 hours, until the jellyfish hardening;

C. extract: the jellyfish of step in taking-up, add 3-5 weight part 0.5M acetic acid heating and refluxing extraction 6-8h, filter, collect filtrate, (chloroform, propyl carbinol mixed solution (chloroform: propyl carbinol=4:1)) stir, and be standing to add isopyknic Seavg reagent in the filtrate;

D. alcohol precipitation: get supernatant liquor and add 2 weight part 95%(v/v after standing demix) ethanol stirs, and standingly makes layering, filters;

E. get filtrate, repeat the d step once;

F. dry: with the filtrate that obtains after upper step ethanol precipitation twice, carry out vacuum-drying, vacuum tightness maintains 0.1MPa, minute two stage dryings, fs keeps 85 ℃ of temperature, and the time kept 1 hour, then heats up, 115 ℃ of subordinate phase temperature, the time kept 3 hours, got product.