CN102000115A - Method for preparing abalone shell pearl layer powder - Google Patents
Method for preparing abalone shell pearl layer powder Download PDFInfo
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- CN102000115A CN102000115A CN2010105681805A CN201010568180A CN102000115A CN 102000115 A CN102000115 A CN 102000115A CN 2010105681805 A CN2010105681805 A CN 2010105681805A CN 201010568180 A CN201010568180 A CN 201010568180A CN 102000115 A CN102000115 A CN 102000115A
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Abstract
The invention provides a method for preparing abalone shell pearl layer powder. The method comprises the following steps of: soaking abalone shells into acid solution to obtain abalone shell pearl layers, performing grinding, acid dissolution and alkali soaking, and dissolving in acid solution to obtain the abalone shell pearl layer powder. Compared with the prior process for preparing the abalone shell pearl layer powder, the method of the invention has the advantages that: the obtained abalone shell pearl layer powder keeps all components of the abalone shell pearl layers, and the soluble powder keeps most components of a biological sample. Compared with a water-soluble pearl powder production method, the method has simple preparation process, and can greatly reduce the production cost and relieve environmental pollution and energy consumption.
Description
Technical field
The present invention relates to a kind of preparation method of abalone shells Margarita layer powder.
Background technology
Nacreous layer claims (hypostracum) at the bottom of the shell again, is the structure of Mollusca (as freshwater mussel etc.) shell innermost layer, is rich in gloss usually, and the refractive power reflection is strong.
Margarita layer powder (nacre powder) is to grind into powder by layer segment in the Mollusca shell, Margarita layer powder and Margarita powder are homologous, contained composition is identical with Margarita, mainly be calcium, glucide, several amino acids and a small amount of trace element, the angle glutelin that is wherein contained has the several amino acids composition that human body self can not produce formation.Margarita layer powder has multi-efficiencies such as flush coat speckle dispelling, treatment skin allergy, acne removing, skin moistening.
The cost of Margarita layer powder itself is very low, in order to obtain nacreous layer, must remove the horny layer and the intermediary prismatic layer (its calcium carbonate composition is the calcite crystal structure) of shell outer surface, optimal making approach is to use physical method to grind off part behind outer and the middle level as raw material, but this processing method can increase the technology cost.So now actual situation is: a lot of factories pulverize after only removing the horny layer of outside with strong alkaline substance such as NaOH etc., and with the Margarita layer powder that these methods with low cost obtain, its composition is easy to destroyedly fall the big heavy discount of medical value meeting.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of abalone shells Margarita layer powder, preparation technology is simple for this method, can significantly reduce production costs, and the Margarita layer powder that obtains has better water solubility, and has kept most of component of nacreous layer.
To achieve these goals, the technical scheme taked of the present invention is as follows:
(1) use the 10-50%(volume ratio) acid solution soaked abalone shells 6-12 hour, peeled off the abalone shells entry material then, reuse 2-5%(volume ratio) hydrogen peroxide dipping abalone shells 1-2 hour, obtained nacreous abalone shells nacreous layer;
(2) the abalone shells nacreous layer is pulverized,, obtained abalone shells nacreous layer grounds travel with the screening of 200 mesh filter screens;
(3) abalone shells nacreous layer grounds travel is dissolved in the acetic acid or lactic acid solution of 2-10% (volume ratio), and continues stirring until and no longer produce bubble, the centrifugal 10-30min of 5000-8000rpm collects supernatant and precipitation respectively then; With the supernatant concentration lyophilizing, obtain powder P
1
(4) precipitation that step (3) is collected was soaked 3-7 days under 80-120 ℃ of temperature with NaOH or the KOH solution of 40-60% (weight ratio), and the centrifugal 15min of 6000-8000rpm collects supernatant and precipitation respectively then; The supernatant dialysis to being neutral, concentrated freeze-dried then, is obtained powder P
2
(5) precipitate that step (4) is collected is dissolved in the 2-20%(volume ratio) acetum in, the centrifugal 10min of 5000-8000rpm gets supernatant; Regulate supernatant to solution with alkali liquor and be neutral, the centrifugal 15min of 6000-8000rpm gets precipitation then; Precipitate with deionized water is cleaned several times, remove moisture then, obtain powder P
3
(6) mix powder P
1, P
2And P
3, obtain the abalone shells Margarita layer powder.
Wherein the described acid solution of step (1) is acetic acid, lactic acid, hydrogen peroxide, hydrochloric acid, nitric acid, oxalic acid, acetone acid, formic acid, benzoic acid or propanoic acid.
The described alkali liquor of step (5) is NaOH or KOH solution.
Abalone shells Margarita layer powder of the present invention dissolves in slightly acidic solution, and dissolubility is better, and this preparation method is equally applicable to the preparation of other shell pearl layer powder.
The shell main component is made up of inorganic mineral and organic substrate, the inorganic matter main component is a calcium carbonate, calcium carbonate with acid solution dissolving shell top layer, can remove the horny layer and the prismatic layer of shell outer surface, reach and separate the purpose that obtains shell pearl layer, as shown in Figure 1, the present invention's separation has obtained nacreous abalone shells nacreous layer, utilize X-ray diffraction (XRD) to detect Hepu Margarita and abalone shells nacreous layer crystal crystalline structure, as shown in Figure 2, illustrate that structurally there is higher similarity in both, can determine that the sample that obtains by this method is the abalone shells nacreous layer really; Soak shell pearl layer with acetic acid or lactic acid solution and can obtain dissolvable matrixs such as nacreous layer activated calcium, trace element and aminoacid, polysaccharide: handle the insoluble Organic substance of nacreous layer with strong alkali solution, formed the solubility shell pearl layer powder of full constituent with above-mentioned soluble component.
Abalone shells Margarita layer powder of the present invention also contains the various active factor except that aminoacid that contains multiple needed by human body and various trace elements.These compositions can strengthen the human epidermal cell viability, enhance metabolism, and endocrine regulation, delaying decrepitude of skin, thus play the magical effect of looks improving and the skin nourishing.Soluble Margarita layer powder increased bioavailability greatly, and it has not only kept most nacreous layer pure natural components and has greatly improved the absorbance of human body to soluble component again.
Remarkable advantage of the present invention:
With respect to the preparation technology of existing abalone shells Margarita layer powder, the abalone shells Margarita layer powder that the present invention obtains has kept abalone shells nacreous layer all components, and soluble powder has then kept most of component of this biological sample.Compare with the water-soluble pearl powder producing method, preparation technology of the present invention is simple, can significantly reduce production costs, and alleviate pollution and energy resource consumption to environment.
Description of drawings
Fig. 1 separates the abalone shells nacreous layer that obtains and does not contrast with removing cuticular abalone shells for the present invention, wherein A is the abalone shells nacreous layer, and B is not for removing cuticular abalone shells, and C is the Hepu Margarita;
Fig. 2 measures Hepu Margarita and the abalone shells nacreous layer crystal crystal form data collection of illustrative plates that obtains for the origin software processes with X-ray diffraction (XRD), wherein A represents that the Y value adds the XRD figure spectrum of 500 Hepu Margarita, and B represents the XRD figure spectrum of the abalone shells nacreous layer that the present invention prepares.
The specific embodiment
Below be several instantiation of the present invention, further specify the present invention, but the present invention be not limited only to this.
Embodiment 1
The preparation method of abalone shells Margarita layer powder of the present invention, concrete steps are as follows:
(1) use the 10%(volume ratio) hydrochloric acid solution soaked abalone shells 10 hours, peeled off the abalone shells extexine then, reuse 2%(volume ratio) hydrogen peroxide dipping abalone shells 2 hours, obtained nacreous abalone shells nacreous layer;
(2) the abalone shells nacreous layer is pulverized,, obtained abalone shells nacreous layer grounds travel with the screening of 200 mesh filter screens;
(3) abalone shells nacreous layer grounds travel is dissolved in the acetum of 2% (volume ratio), and continues stirring until and no longer produce bubble, the centrifugal 30min of 6000rpm collects supernatant and precipitation respectively then; With the supernatant concentration lyophilizing, obtain powder P
1
(4) precipitation that step (3) is collected was soaked 3 days under 120 ℃ of temperature with the NaOH solution of 40% (weight ratio), and the centrifugal 15min of 6000rpm collects supernatant and precipitation respectively then; Supernatant is dialysed to being neutral, concentrated freeze-dried then with deionized water, obtain powder P
2
(5) precipitate that step (4) is collected is dissolved in the 5%(volume ratio) acetum in, the centrifugal 10min of 5000rpm gets supernatant; Regulate supernatant to solution with NaOH solution and be neutral, the centrifugal 15min of 6000rpm gets precipitation then; Precipitate with deionized water is cleaned several times, and moisture is removed in 35-60 ℃ of oven dry then, obtains powder P
3
(6) mix powder P
1, P
2And P
3, obtain the abalone shells Margarita layer powder.
Embodiment 2
The preparation method of abalone shells Margarita layer powder of the present invention, concrete steps are as follows:
(1) use the 50%(volume ratio) lactic acid soaked abalone shells 6 hours, peeled off the abalone shells extexine then, reuse 5%(volume ratio) hydrogen peroxide dipping abalone shells 1 hour, obtained nacreous abalone shells nacreous layer;
(2) the abalone shells nacreous layer is pulverized,, obtained abalone shells nacreous layer grounds travel with the screening of 200 mesh filter screens;
(3) abalone shells nacreous layer grounds travel is dissolved in the lactic acid solution of 10% (volume ratio), and continues stirring until and no longer produce bubble, the centrifugal 10min of 8000rpm collects supernatant and precipitation respectively then; With the supernatant concentration lyophilizing, obtain powder P
1
(4) precipitation that step (3) is collected was soaked 7 days under 80 ℃ of temperature with the KOH solution of 60% (weight ratio), and the centrifugal 15min of 8000rpm collects supernatant and precipitation respectively then; The supernatant dialysis to being neutral, concentrated freeze-dried then, is obtained powder P
2
(5) precipitate that step (4) is collected is dissolved in the 20%(volume ratio) acetum in, the centrifugal 10min of 8000rpm gets supernatant; Regulate supernatant to solution with NaOH solution and be neutral, the centrifugal 15min of 8000rpm gets precipitation then; Precipitate with deionized water is cleaned several times, remove moisture then, obtain powder P
3
(6) mix powder P
1, P
2And P
3, obtain the abalone shells Margarita layer powder.
Embodiment 3
The preparation method of abalone shells Margarita layer powder of the present invention, concrete steps are as follows:
(1) use the 25%(volume ratio) acetum soaked abalone shells 9 hours, peeled off the abalone shells entry material then, reuse 3%(volume ratio) hydrogen peroxide dipping abalone shells 1.5 hours, obtained nacreous abalone shells nacreous layer;
(2) the abalone shells nacreous layer is pulverized,, obtained abalone shells nacreous layer grounds travel with the screening of 200 mesh filter screens;
(3) abalone shells nacreous layer grounds travel is dissolved in the lactic acid solution of 6% (volume ratio), and continues stirring until and no longer produce bubble, the centrifugal 20min of 6000rpm collects supernatant and precipitation respectively then; With the supernatant concentration lyophilizing, obtain powder P
1
(4) precipitation that step (3) is collected was soaked 5 days under 100 ℃ of temperature with the NaOH solution of 50% (weight ratio), and the centrifugal 15min of 6000rpm collects supernatant and precipitation respectively then; The supernatant dialysis to being neutral, concentrated freeze-dried then, is obtained powder P
2
(5) precipitate that step (4) is collected is dissolved in the 10%(volume ratio) acetum in, the centrifugal 10min of 6000rpm gets supernatant; Regulate supernatant to solution with KOH solution and be neutral, the centrifugal 15min of 6000rpm gets precipitation then; Precipitate with deionized water is cleaned several times, and moisture is removed in oven dry then, obtains powder P
3
(6) mix powder P
1, P
2And P
3, obtain the abalone shells Margarita layer powder.
?
Claims (3)
1. the preparation method of an abalone shells Margarita layer powder, it is characterized in that: the concrete steps of described preparation method are as follows:
(1) use the 10-50%(volume ratio) acid solution soaked abalone shells 6-12 hour, peeled off the abalone shells entry material then, reuse 2-5%(volume ratio) hydrogen peroxide dipping abalone shells 1-2 hour, obtained nacreous abalone shells nacreous layer;
(2) the abalone shells nacreous layer is pulverized,, obtained abalone shells nacreous layer grounds travel with the screening of 200 mesh filter screens;
(3) abalone shells nacreous layer grounds travel is dissolved in the acetic acid or lactic acid solution of 2-10% (volume ratio), and continues stirring until and no longer produce bubble, the centrifugal 10-30min of 5000-8000rpm collects supernatant and precipitation respectively then; With the supernatant concentration lyophilizing, obtain powder P
1
(4) precipitation that step (3) is collected was soaked 3-7 days under 80-120 ℃ of temperature with NaOH or the KOH solution of 40-60% (weight ratio), and the centrifugal 15min of 6000-8000rpm collects supernatant and precipitation respectively then; The supernatant dialysis to being neutral, concentrated freeze-dried then, is obtained powder P
2
(5) precipitate that step (4) is collected is dissolved in the 2-20%(volume ratio) acetum in, the centrifugal 10min of 5000-8000rpm gets supernatant; Regulate supernatant to solution with alkali liquor and be neutral, the centrifugal 15min of 6000-8000rpm gets precipitation then; Precipitate with deionized water is cleaned several times, remove moisture then, obtain powder P
3
(6) mix powder P
1, P
2And P
3, obtain the abalone shells Margarita layer powder.
2. the preparation method of abalone shells Margarita layer powder according to claim 1 is characterized in that: the described acid solution of step (1) is acetic acid, lactic acid, hydrogen peroxide, hydrochloric acid, nitric acid, oxalic acid, acetone acid, formic acid, benzoic acid or propanoic acid.
3. the preparation method of abalone shells Margarita layer powder according to claim 1 is characterized in that: the described alkali liquor of step (5) is NaOH or KOH solution.
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|---|---|---|---|
| CN2010105681805A CN102000115B (en) | 2010-12-01 | 2010-12-01 | Method for preparing abalone shell pearl layer powder |
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| CN2010105681805A CN102000115B (en) | 2010-12-01 | 2010-12-01 | Method for preparing abalone shell pearl layer powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103623005A (en) * | 2013-12-05 | 2014-03-12 | 福州福大海洋科技有限公司 | Abalone shell pearl layer powder and processing method thereof |
| CN103638048A (en) * | 2013-12-20 | 2014-03-19 | 福州大学 | Acid-pickled concha haliotidis and preparation method thereof |
| CN104940025A (en) * | 2015-07-17 | 2015-09-30 | 福州大学 | Abalone shell water-soluble matrix and preparation method and application thereof |
| CN109244318A (en) * | 2018-09-03 | 2019-01-18 | 中国科学技术大学 | The preparation method of porous aragonite structure micron chip, ceramic diaphragm and preparation method thereof |
| CN109851690A (en) * | 2019-03-14 | 2019-06-07 | 福州大学 | A kind of preparation method of shell pearl layer chitin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101455400A (en) * | 2008-11-15 | 2009-06-17 | 山东好当家海洋发展股份有限公司 | Sea-ear shell calcium lactate processing method |
| CN100581554C (en) * | 2006-07-17 | 2010-01-20 | 大连太平洋海龙科技有限公司 | Abalone shell calcium complement agent |
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2010
- 2010-12-01 CN CN2010105681805A patent/CN102000115B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100581554C (en) * | 2006-07-17 | 2010-01-20 | 大连太平洋海龙科技有限公司 | Abalone shell calcium complement agent |
| CN101455400A (en) * | 2008-11-15 | 2009-06-17 | 山东好当家海洋发展股份有限公司 | Sea-ear shell calcium lactate processing method |
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| 《食品科学》 20100930 梁红宝等 鲍鱼壳水溶性基质抗氧化活性研究 113-116 1-3 第31卷, 第9期 2 * |
| 《齐鲁渔业》 20010131 程振明等 水溶性鲍壳珍珠层粉的制备工艺 27-28 1-3 第18卷, 第1期 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103623005A (en) * | 2013-12-05 | 2014-03-12 | 福州福大海洋科技有限公司 | Abalone shell pearl layer powder and processing method thereof |
| CN103623005B (en) * | 2013-12-05 | 2017-04-12 | 福州福大海洋科技有限公司 | Abalone shell pearl layer powder and processing method thereof |
| CN103638048A (en) * | 2013-12-20 | 2014-03-19 | 福州大学 | Acid-pickled concha haliotidis and preparation method thereof |
| CN104940025A (en) * | 2015-07-17 | 2015-09-30 | 福州大学 | Abalone shell water-soluble matrix and preparation method and application thereof |
| CN109244318A (en) * | 2018-09-03 | 2019-01-18 | 中国科学技术大学 | The preparation method of porous aragonite structure micron chip, ceramic diaphragm and preparation method thereof |
| CN109244318B (en) * | 2018-09-03 | 2020-12-25 | 中国科学技术大学 | Preparation method of porous aragonite structure micron sheet, ceramic diaphragm and preparation method of ceramic diaphragm |
| CN109851690A (en) * | 2019-03-14 | 2019-06-07 | 福州大学 | A kind of preparation method of shell pearl layer chitin |
| CN109851690B (en) * | 2019-03-14 | 2021-11-02 | 福州大学 | Preparation method of shell nacreous layer chitin |
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| CN102000115B (en) | 2012-04-11 |
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