CN106615597A - Method for preparing red snapper fish scale collagen protein - Google Patents

Abstract

The invention relates to a method for preparing red snapper fish scale collagen protein and belongs to the technical field of food processing. According to the method, the red snapper fish scale collagen protein is taken as a raw material, tap water is utilized to clean dry fish scale, impurities on the surface of the fish scale is removed, the fish scale is air-dried, and the red snapper fish scale collagen protein is obtained after hydrochloric acid de-ashing, cleaning, gelatin extraction, filtration, deionization, concentration and powder spraying. The prepared red snapper fish scale collagen protein has characteristics of ordinary protein and can be applied to industries of foods, packaging and cosmetics and the like, and the method for preparing the red snapper fish scale collagen protein is low in production cost and short in cycle.

Description

A kind of preparation method of red snapper fish scale collagen protein powder

technical field

The invention relates to a preparation method of red snapper scale collagen powder, which belongs to the technical field of food processing.

Background technique

Collagen is a macromolecular protein ubiquitous in animals, mainly in the connective tissue of animals, such as pigskin, pig Achilles tendon, beef bone, and beef tendon contain a large amount of collagen. Collagen has good biocompatibility, biodegradability, and low antigenicity, so it is widely used in food, medicine, cosmetics, and packaging industries. At present, the source of collagen on the market is mainly mammals. The outbreaks of mad cow disease and foot-and-mouth disease in recent years have restricted the use of mammalian collagen. Therefore, people began to search for new protein sources, such as extracting collagen from fish scales and fish skins. Extracting collagen from fish scales can not only solve the food safety problems of collagen that may be caused by foot-and-mouth disease and mad cow disease, but also break the restrictions of religious beliefs and customs and cultures to meet the needs of different ethnic groups.

Red snapper is a deep-sea fish that lives at about 200 meters, and is mainly distributed in China's Bohai Sea, Yellow Sea, East China Sea, and South China Sea. Red snapper scales contain a large amount of collagen, which is composed of nearly 20 kinds of amino acids such as hydroxyproline, glycine, alanine, tryptophan, etc. Among them, hydroxyproline has a good effect on the skin, while glycine It has the effect of inhibiting tumor spread. Compared with other fish scales, the content of hydroxyproline in red snapper scales is several times that of other fish species. In addition, the living environment temperature of red snapper is relatively low, so that the calories of collagen are low. At the same time will not add fat. In addition, the collagen extracted from fish scales is obviously superior to mammalian collagen in some aspects, such as low antigenicity and hypoallergenicity. Therefore, aquatic collagen has a tendency to replace mammalian collagen.

Contents of the invention

The object of the present invention is to provide a preparation method of red snapper fish scale collagen powder, which improves the comprehensive utilization rate of red snapper fish scales and improves the extraction process of fish scale protein.

According to the technical scheme provided by the present invention, a preparation method of red snapper fish scale collagen powder, it uses dried red snapper fish scales as raw material, cleans the dried fish scales with tap water, removes impurities on the surface of the fish scales, dries them, and then passes through Red snapper scale collagen powder is prepared after hydrochloric acid deliming, cleaning, gel extraction, filtration, deionization, concentration and dusting; the specific steps are as follows:

(1) Cleaning of dried fish scales: Clean the dried fish scales of red snapper with clean water, remove surface impurities, and dry at room temperature;

(2) Deliming treatment: use 0.2-0.5mol/L hydrochloric acid to soak and deash the red snapper scales obtained in step (1) in a soaking tank for 10-16 hours, wherein the red snapper scales and hydrochloric acid have a solid-to-liquid ratio of For 1g fish scale: 10-14mL hydrochloric acid, stir while soaking, the stirring speed is 20-60r/min;

(3) Cleaning: Clean the dried red snapper fish scales obtained in step (2) with clean water for 3-5 times, and prepare for gel extraction;

(4) Glue extraction: After adding 10-14 times the volume of tap water to the cleaned red snapper scales in step (3), heat the rubber extraction tank to 70-100°C, stir at 40-80r/min, and keep for 3 After -5h, the solid scales were filtered out to obtain the red snapper scale collagen solution;

(5) Centrifugal filtration: centrifuge the red snapper scale collagen solution extracted in step (4) with a centrifuge, and vacuum filter the collagen solution with a microporous filter membrane and a vacuum pump;

(6) Deionization: Pack the treated anion and cation resins into two columns respectively, and deionize the collagen solution after centrifugal filtration obtained in step (5) through anion and cation columns;

(7) Concentration: Concentrate the collagen solution prepared in step (6) to 28%-40% in the concentration device;

(8) Powder spraying: use a spray dryer for powder spraying, the inlet hot air temperature is 160-200°C, and the outlet hot air temperature is 60-100°C, and the red snapper scale collagen powder is obtained.

The centrifuge speed during centrifugal filtration in step (5) is 4000-6000r/min, and the centrifugation time is 15-20min; the microporous membrane is a mixed cellulose microporous membrane with a pore size of 20-50 μm; the vacuum pump is - 0.1MPa circulating water vacuum pump.

Beneficial effects of the present invention: the red snapper fish scale protein prepared by the present invention has the characteristics of general protein, and can be applied to industries such as food, packaging, cosmetics, etc., and the preparation process of fish scale collagen adopted by the present invention has low production cost and short cycle time. short.

detailed description

The present invention will be further described below in conjunction with specific embodiments.

The instrument that adopts in the present invention has the FC104 electronic analytical balance that Shanghai Precision Scientific Instrument Company manufactures; The PHS-25 type acidity meter that Shanghai Lei Magnetic Instrument Factory manufactures; The 876A-S2 vacuum oven that Shanghai Pudong Rongfeng Scientific Instrument Co., Ltd. manufactures; RJ-TDL-50A centrifuge manufactured by Wuxi Ruijiang Analytical Instrument Co., Ltd.; HN-15-0510 reaction kettle; master-53α concentration meter of Aituo Company.

The preparation method of embodiment 1 red snapper fish scale protein, comprises the steps:

(1) Cleaning of dried fish scales: Weigh 600g of dried fish scales, wash the dried fish scales with clean water, remove surface impurities, and dry them in the air.

(2) Deliming treatment: Weigh 500g of fish scales after cleaning and drying, put the scales into a soaking tank, then add 5L0.2mol/L hydrochloric acid, soak and deash the red snapper scales for 10 hours, and soak them while soaking. Stir at a speed of 20r/min.

(3) Cleaning: Wash the fish scales treated in (2) three times with clean water, and prepare for gel extraction.

(4) Gel extraction: Add 10 times the volume of tap water to the cleaned fish scales, heat the gel tank to 70°C, stir at 40r/min, keep for 3 hours, and filter out the solid fish scales to obtain a collagen solution.

(5) Centrifugal filtration: Centrifuge the extracted fish scale collagen solution with a centrifuge, vacuum filter the collagen solution with a microporous filter membrane and a vacuum pump, and collect the filtrate. The rotating speed of the centrifuge during filtration is 4000r/min, and the centrifugation time is 20min; the microporous membrane is a mixed cellulose microporous membrane with a pore size of 20 μm; the vacuum pump is a circulating water vacuum pump of -0.1MPa.

(6) Deionization: The treated anion and cation resins are respectively installed in two columns, and the glue solution passes through the anion and anion columns in turn to remove the anion and cation heteroions in the collagen solution.

(7) Concentration: Concentrate the prepared collagen solution to 28% with a three-effect concentration device.

(8) Powder spraying: use a spray dryer for powder spraying, the inlet hot air temperature is 160°C, and the outlet hot air temperature is 80°C to prepare red snapper scale collagen powder.

The preparation method of embodiment 2 red snapper fish scale protein, comprises the steps:

(1) Cleaning of dried fish scales: Weigh 600g of dried fish scales, wash the dried fish scales with clean water, remove surface impurities, and dry them in the air.

(2) Deliming treatment: Weigh 500g of dried fish scales, put the fish scales into a soaking tank, then add 5L of 0.5mol/L hydrochloric acid, soak and deash the red snapper scales for 16 hours, and stir while soaking , the speed is 60r/min.

(3) Cleaning: After cleaning the fish scales treated in (2) 5 times with clean water, prepare for gel extraction.

(4) Glue extraction: After adding 14 times the volume of tap water to the cleaned fish scales, heat the rubber extraction tank to 100°C with a stirring speed of 60r/min, keep for 5 hours, and then filter out the solid fish scales to obtain a collagen solution.

(5) Centrifugal filtration: Centrifuge the extracted fish scale collagen solution with a centrifuge, vacuum filter the collagen solution with a microporous filter membrane and a vacuum pump, and collect the filtrate. The rotating speed of the centrifuge during filtration is 6000r/min, and the centrifugation time is 15min; the microporous membrane is a mixed cellulose microporous membrane with a pore size of 50 μm; the vacuum pump is a circulating water vacuum pump of -0.1MPa.

(6) Deionization: The treated anion and cation resins are respectively installed in two columns, and the glue solution passes through the anion and anion columns in turn to remove the anion and cation heteroions in the collagen solution.

(7) Concentration: Concentrate the prepared collagen solution to 40% with a three-effect concentration device.

(8) Powder spraying: use a spray dryer for powder spraying, the inlet hot air temperature is 200°C, and the outlet hot air temperature is 100°C to prepare red snapper scale collagen powder.

The preparation method of embodiment 3 red snapper fish scale protein, comprises the steps:

(1) Cleaning of dried fish scales: Weigh 600g of dried fish scales, wash the dried fish scales with clean water, remove surface impurities, and dry them in the air.

(2) Deliming treatment: weigh 500g of dried fish scales, put the fish scales into a soaking tank, then add 5L of 0.4mol/L hydrochloric acid, soak and deash the red snapper scales for 10 hours, and stir while soaking , the speed is 50r/min.

(3) Cleaning: After cleaning the fish scales treated in (2) 4 times with clean water, prepare for gel extraction.

(4) Gel extraction: After adding 12 times the volume of tap water to the cleaned fish scales, heat the gel extraction tank to 80°C with a stirring speed of 50r/min, keep for 4 hours, and then filter out the solid fish scales to obtain a collagen solution.

(5) Centrifugal filtration: Centrifuge the extracted fish scale collagen solution with a centrifuge, vacuum filter the collagen solution with a microporous filter membrane and a vacuum pump, and collect the filtrate. The rotating speed of the centrifuge during filtration is 5000r/min, and the centrifugation time is 18min; the microporous membrane is a mixed cellulose microporous membrane with a pore size of 30 μm; the vacuum pump is a circulating water vacuum pump of -0.1MPa.

(6) Deionization: The treated anion and cation resins are respectively installed in two columns, and the glue solution passes through the anion and anion columns in turn to remove the anion and cation heteroions in the collagen solution.

(7) Concentration: Concentrate the prepared collagen solution to 35% with a three-effect concentration device.

(8) Powder spraying: use a spray dryer for powder spraying, the inlet hot air temperature is 180°C, and the outlet hot air temperature is 80°C to prepare red snapper scale collagen powder.

Claims (2)

1. a kind of preparation method of Red snapper fish scale collagen powder, is characterized in that：It is used certainly with Red snapper dry fish scale as raw material Water is cleaned to dry fish scale, removes the impurity on fish scale surface, is dried, then through ash removal by HCl, clean, carry glue, filter, go Ion, concentrate and prepare after dusting Red snapper fish scale collagen powder；Comprise the following steps that：

（1）Dry fish scale is cleaned：Red snapper dry fish scale is cleaned with clear water, removes the impurity on surface, normal temperature dries；

（2）Deliming process：With the hydrochloric acid of 0.2-0.5mol/L in steeping tank to step（1）The Red snapper fish scale of gained is soaked Bubble deliming processes 10-16h, and wherein Red snapper fish scale and hydrochloric acid solid-to-liquid ratio is 1g fish scales:10-14mL hydrochloric acid, stirs in immersion, Speed of agitator is 20-60r/min；

（3）Cleaning：With clear water to step（2）Red snapper dry fish scale obtained by process carries out cleaning 3-5 time, and preparation carries glue；

（4）Carry glue：In step（3）Add after 10-14 times of volume of tap water in Red snapper fish scale after cleaning, the heating of glue tank will be proposed To 70-100 DEG C, mixing speed is 40-80r/min, keeps filtering out the prepared Red snapper fish glue from scale stoste of solid fish scale after 3-5h；

（5）Centrifugal filtration：With centrifuge to step（4）The Red snapper fish glue from scale stoste of extraction is centrifuged, with miillpore filter and Vavuum pump carries out vacuum filtration to collagen solution；

（6）Deionization：The yin and yang resin for processing is attached separately in two pillars, by step（5）After gained centrifugal filtration Collagen solution passes through zwitterion post deionization；

（7）Concentration：By step（6）Obtained collagen solution is concentrated into 28%-40% in enrichment facility；

（8）Dust：Dusted using spray dryer, import hot blast temperature is 160-200 DEG C, exports hot blast temperature 60- 100 DEG C, that is, Red snapper fish scale collagen powder is obtained.

2. the preparation method of Red snapper fish scale collagen powder as claimed in claim 1, is characterized in that：Step（5）In be centrifuged Centrifuge speed during filter is 4000-6000r/min, and centrifugation time is 15-20min；It is 20-50 that the miillpore filter is aperture μm cellulose mixture miillpore filter；Vavuum pump is the circulating water type vavuum pump of -0.1MPa.