CN101250218A - Method for preparing cod collagen - Google Patents
Method for preparing cod collagen Download PDFInfo
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- CN101250218A CN101250218A CNA2007100140312A CN200710014031A CN101250218A CN 101250218 A CN101250218 A CN 101250218A CN A2007100140312 A CNA2007100140312 A CN A2007100140312A CN 200710014031 A CN200710014031 A CN 200710014031A CN 101250218 A CN101250218 A CN 101250218A
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Abstract
A preparation method of cod skin collagen, which is characterized in that the method comprises thawing frozen cod skin sample, removing impurity, washing via tap water and breaking mechanically, adding pretreated cod skin into hydrochloride to be immerged, mixing uniformly, adjusting pH value, bathing in water and centrifugally treating, extracting the left slag via hot water, centrifugally treating, combining extracts, concentrating and drying to obtain collagen product. The invention has short production period, whose product is white or light yellow, has high quality and is soluble in water.
Description
Technical field
The present invention relates to method with the hydrochloric acid extraction cod collagen.The present invention makes full use of aquatic resources, turns waste into wealth, and extracts the with short production cycle of collagen protein, product shows white or faint yellow, and quality is good, and is very easily water-soluble, can be used for fields such as health care, food.
Background technology
China is as fishery big country, and the fishery products total amount is the trend that increases year by year.Along with the raising of people's living standard, fishery products are except a small amount of marketing fresh, and major part is processed into finished product or work in-process sale such as fillet, fish gruel, fish ball.Can produce a large amount of fish head, fin, fish-skin the like waste in the course of processing, these wastes have only small part to be processed to low value products such as fish meal at present, more go out of use and cause environmental pollution.Therefore, the optimization utilization of low value Living marine resources and high-valued exploitation are crucial.
The present purposes of collagen protein macromolecular compound is very extensive, spread all over fields such as medicine, chemical industry, food, product has various different shapeies and purposes, and according to its shape, cooperate every purposes, wherein the application of biomedical material and pharmaceutical preparations is the problem of popular research.Recently, discover that both at home and abroad collagen content is higher in the fish-skin, the collagen protein in the fish-skin dissolves in neutral salt solution or acidic solution, obtains soluble collagen solution than being easier to modulation, and this has enriched the source of collagen protein greatly.
Extracting method to collagen of fish skin has at present: (1) uses the alkali acid treatment successively, makes the abundant swelling of collagen protein, uses hot-water extraction.The cod protein solution muddiness that these class methods are extracted, egg white solid gray and solubility are bad.(2) alkaline process is handled, and needs to soak a couple of days, hot-water extraction with calcium chloride, lime white.The collagen protein hydroxyproline content height that this method is extracted, quality is good, but the production cycle is long.
So far do not see report as yet with the hydrochloric acid extraction cod collagen.
Summary of the invention
The object of the invention: provide a kind of method with the hydrochloric acid extraction cod collagen, it can overcome the above-mentioned defective of prior art.
With the method for acid extraction collagen of fish skin, it is characterized in that the fish-skin sample of cold storage is thawed, reject non-fish-skin impurity, clean with tap water, machinery cuts and fragmentates.A certain amount of pretreated fish-skin is added 4～6 times of volume 0.2%～0.5% salt acid soak 3～6h, homogenate; The homogenate volume is added to 6～8 times, and the pH value is adjusted to about 4～5, heating in water bath to 70～80 ℃, and under this temperature, constantly stir extracting 6～8h, centrifuging; Residue adds the water of 1～4 times of volume, in 70～80 ℃ of extractings 2～4 hours, centrifuging; Merge extracted twice liquid, concentrate drying obtains the collagen goods.
Beneficial effect: the present invention utilizes monoprotic acid swelling cod collagen, and the swelling effect is better; Collagen protein is extracted in pH value control with extracting solution, removes noncollagen protein.It is with short production cycle that this method is extracted collagen protein, product shows white or faint yellow, and quality is good, and is very easily water-soluble.
Embodiment
The fish-skin sample of cold storage is thawed, reject non-fish-skin impurity, clean with tap water, machinery cuts and fragmentates.A certain amount of pretreated fish-skin is added 4 times of volume 0.25% salt acid soak 4h, homogenate; The homogenate volume is added to 8 times, and the pH value adjusts to 4.0, heating in water bath to 70 ℃, and under this temperature, constantly stir extracting 6h, centrifugal (4000r/min, 15min) filters; Residue adds the water of 2 times of volumes, in 70 ℃ of extractings 2 hours, centrifuging; Merge extracted twice liquid, concentrated, spraying drying obtain the collagen goods.
The advantage of cod collagen product of the present invention is: make full use of aquatic resources, turn waste into wealth, both reduced environmental pollution, improved the quality of collagen protein again.Technology is simple to operation, with short production cycle, and cost is low, quality good.
Claims (4)
1. the preparation method of cod collagen.The cod skin sample of cold storage is thawed, reject non-fish-skin impurity, clean with tap water, machinery cuts and fragmentates.A certain amount of pretreated fish-skin is added 4～6 times of volume 0.2%～0.5% salt acid soak 3～6h, homogenate; The homogenate volume is added to 6～8 times, and the pH value is adjusted to about 4～5, heating in water bath to 70～80 ℃, and under this temperature, constantly stir extracting 6～8h, centrifuging; Residue adds the water of 1～4 times of volume, in 70～80 ℃ of extracting 2～4h, centrifuging; Merge extracted twice liquid, concentrate drying obtains the collagen goods.
2. preparation method according to claim 1, the raw material that it is characterized in that preparing collagen protein is a cod skin.
3. preparation method according to claim 1 is characterized in that fish-skin elder generation uses the salt acid-swellable, removes noncollagen protein, at last extracting collagen protein under water bath condition again.
4. preparation method according to claim 1 is characterized in that concentration of hydrochloric acid is 0.2%～0.5%, and addition is 4～6 times of fish-skin volume, and soak time is 3～6h; Removing the noncollagen protein condition is: pH value about 4～5, and 70～80 ℃ of temperature, the time is 6～8h; The hot-water extraction condition is: amount of water is 1～4 times of residue volume, 70～80 ℃ of temperature, and the time is 2～4h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100140312A CN101250218A (en) | 2007-03-22 | 2007-03-22 | Method for preparing cod collagen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100140312A CN101250218A (en) | 2007-03-22 | 2007-03-22 | Method for preparing cod collagen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101250218A true CN101250218A (en) | 2008-08-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100140312A Pending CN101250218A (en) | 2007-03-22 | 2007-03-22 | Method for preparing cod collagen |
Country Status (1)
| Country | Link |
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| CN (1) | CN101250218A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735304B (en) * | 2008-11-26 | 2012-06-06 | 大连隆合海洋生物工程技术有限公司 | Method for preparing water-based collagen of codfish skin by ultrasound wave extraction |
| CN102552975A (en) * | 2012-02-28 | 2012-07-11 | 青岛中皓生物工程有限公司 | Tissue engineering human corneal stroma carrier bracket and preparation method thereof |
| CN102559826A (en) * | 2012-02-15 | 2012-07-11 | 胡如桂 | Method for preparing collagen oligopeptide |
| CN103070395A (en) * | 2013-01-25 | 2013-05-01 | 桑植县溇水大鲵生物科技有限公司 | Producing method of giant salamander collagen particle and giant salamander collagen product |
| CN103911415A (en) * | 2014-04-10 | 2014-07-09 | 广州康洛信生物科技有限公司 | Method for safely and efficiently preparing collagen via one-step process |
| CN104774258A (en) * | 2015-04-27 | 2015-07-15 | 山东美佳集团有限公司 | Preparation method of turbot fish skin collagen |
| CN113150118A (en) * | 2021-04-25 | 2021-07-23 | 广州创尔生物技术股份有限公司 | Quick preparation method of non-denatured I type collagen |
| CN113899647A (en) * | 2021-09-27 | 2022-01-07 | 山东海奥斯生物科技有限公司 | Method for detecting content of microcrystalline cellulose in collagen casing |
-
2007
- 2007-03-22 CN CNA2007100140312A patent/CN101250218A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735304B (en) * | 2008-11-26 | 2012-06-06 | 大连隆合海洋生物工程技术有限公司 | Method for preparing water-based collagen of codfish skin by ultrasound wave extraction |
| CN102559826A (en) * | 2012-02-15 | 2012-07-11 | 胡如桂 | Method for preparing collagen oligopeptide |
| CN102552975A (en) * | 2012-02-28 | 2012-07-11 | 青岛中皓生物工程有限公司 | Tissue engineering human corneal stroma carrier bracket and preparation method thereof |
| CN102552975B (en) * | 2012-02-28 | 2013-09-11 | 青岛中皓生物工程有限公司 | Tissue engineering human corneal stroma carrier bracket and preparation method thereof |
| CN103070395A (en) * | 2013-01-25 | 2013-05-01 | 桑植县溇水大鲵生物科技有限公司 | Producing method of giant salamander collagen particle and giant salamander collagen product |
| CN103911415A (en) * | 2014-04-10 | 2014-07-09 | 广州康洛信生物科技有限公司 | Method for safely and efficiently preparing collagen via one-step process |
| CN103911415B (en) * | 2014-04-10 | 2016-06-15 | 广州康洛信生物科技有限公司 | Single stage method prepares the method for collagen protein |
| CN104774258A (en) * | 2015-04-27 | 2015-07-15 | 山东美佳集团有限公司 | Preparation method of turbot fish skin collagen |
| CN113150118A (en) * | 2021-04-25 | 2021-07-23 | 广州创尔生物技术股份有限公司 | Quick preparation method of non-denatured I type collagen |
| CN113899647A (en) * | 2021-09-27 | 2022-01-07 | 山东海奥斯生物科技有限公司 | Method for detecting content of microcrystalline cellulose in collagen casing |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080827 |