CN104894202A - Method for extracting collagen from rabbit skins - Google Patents
Method for extracting collagen from rabbit skins Download PDFInfo
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- CN104894202A CN104894202A CN201510365884.5A CN201510365884A CN104894202A CN 104894202 A CN104894202 A CN 104894202A CN 201510365884 A CN201510365884 A CN 201510365884A CN 104894202 A CN104894202 A CN 104894202A
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Abstract
The invention relates to a method for extracting collagen from rabbit skins. The method includes removing the subcutaneous fat and hair of the rabbit skins, cleaning and shearing the rabbit skins, soaking the preprocessed rabbit skins into acid with the pH of 1.9 for 8-10 hours, crushing the processed rabbit skins into pulp, adding acetic acid with the pH of 1.9 and evenly stirring, adding pepsin accounting for 1% of the mass of the rabbit skins for enzymolysis under 4 DEG C for 4 hours, filtering to obtain filtrate, adding NaOH into the filtrate to adjust pH to 7-8, adding NaCl to the concentration of 4-5mol/L for salting out for 12 hours, centrifuging salting-out products, taking precipitation, adding water for centrifuging until the NaCl is removed completely, taking the precipitation to obtain crude collagen extracts, and freeze drying the crude collagen extracts to obtain the purified collagen.
Description
Technical field
The invention belongs to processing of farm products field, relate to a kind of preparation method of collagen protein, particularly a kind of method extracting collagen protein from rabbit.
Background technology
Collagen protein is the most rich in protein of Mammals in-vivo content, accounts for the 25%-30% of total protein.Collagen protein is widely used in food, makeup, medicine and other fields, and the global output value of collagen protein in 2013 has reached about 2,000 hundred million yuan.
Traditional collagen protein produce main with the osteoderm of the livestock such as ox, pig for raw material production, the outburst of the zoonosis such as mad cow disease and foot and mouth disease in recent years, people throw doubt upon to the security of ox, pig derived collagen protein product, livestock collagen protein and goods thereof are restricted, and European Union has even forbidden using ox derived collagen protein product in food.In addition due to the reason such as religion and custom, some Special section such as kosher and Islam area do not accept pig derived collagen protein product, do not accept the ox derived collagen protein product butchered not according to their requirement and produce yet.At present, utilize aquatic products processing by product, if fish-skin, fish scale, fish-bone etc. are as the surrogate of traditional pig, ox derived collagen albumen, but, comparatively mammal collagen is low for aquatic collagen protein yldeneamino acid content, and comparatively mammalian collagen is slightly poor to cause aquatic collagen protein stability.Therefore, take Novel biological-resource as the development trend that development of raw materials novel collagen product is studied for collagen protein.
Along with growth in the living standard, people more and more pay close attention to the nutritional quality of meat.Rabbit meat because of the nutrition speciality of its " three high and one low " (high protein, high mineral, high digestibility, low in calories, lower fat, low-cholesterol), and does not find the zoonosis of rabbit, and the consumption of people to rabbit meat is increased, and rabbit aquaculture is flourish.2005, China suddenly became world's rabbit meat and produces the first big country, and within 2013, world's rabbit meat ultimate production is about 2,000,000 tons, and China's rabbit meat production is about 800,000 tons, accounts for 40% of world's rabbit meat gross production.But meat rabbit produces a large amount of rabbit in process for processing, but its added value is lower, causes the wasting of resources and environmental pollution.
As can be seen here, according to rabbit characteristic, set up rabbit collagen production process, prepare high-quality collagen, increase rabbit commercial product added value and become the task of top priority.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of method extracting collagen protein from rabbit.
For achieving the above object, the invention provides following technical scheme:
From rabbit, extract a method for collagen protein, it is characterized in that, its concrete steps are as follows:
(1) rabbit pre-treatment: rabbit is cleaned, except subcutaneous lipids, depilation, stripping and slicing, removes foreign protein, then wash;
(2) acid soak: get acetic acid mixing immersion 8 ~ 10h that rabbit that step (1) processed and pH value are 1.85 ~ 1.95, the mass volume ratio of described rabbit and acetic acid is 1:14 ~ 16;
(3) enzymolysis: the rabbit that step (2) processes is smashed in pulpous state, continuing to add pH value is that the acetic acid of 1.85-1.95 stirs again, the mass volume ratio of rabbit and acetic acid is 1:29 ~ 31, in rabbit quality, add the stomach en-of 0.095% ~ 1.005%, under 3 ~ 5 DEG C of temperature condition, enzymolysis 230-250min, filters to get filtrate;
(4) saltout: in filtrate, add NaOH regulate pH to 7 ~ 8, then to add NaCl to concentration be 4 ~ 5mol/L, carries out saltouing 12h;
(5) centrifugal: thing of saltouing is centrifugal, taking precipitate, then add water centrifugal until fully remove NaCl, get precipitation, namely obtain collagen protein crude extract;
(6) freeze-drying: by the freeze-drying of collagen protein crude extract, obtain the collagen protein of purifying.
Preferably, rabbit is cut into 3mm × 3mm block.
Preferably, in described step (3) enzymolysis process, add pH value be 1.9 acetic acid stir, the mass volume ratio of rabbit and acetic acid is 1:30, in rabbit quality, interpolation 1% stomach en-, enzymolysis 240min under 4 DEG C of temperature condition.
Preferably, step (1) described removal foreign protein mode is: be the NaCl solution mixing of 1% by rabbit and massfraction with mass volume ratio 1:4 ~ 6, soak 350 ~ 370min, every 120min changes a not good liquor.
Preferably, step (5) each centrifugal 10min under rotating speed is 5000r/min condition.
Beneficial effect of the present invention is: institute of the present invention establishment method utilizes stomach en-to extract collagen protein in rabbit, this process characteristic is that collagen protein prepared by raw material with rabbit, avoids other mammalian source pigs, bovine collagen protein product uses restricted problem; And on Product Process, shorten the extraction time of collagen protein greatly, fundamentally improve the extraction long present situation consuming time of collagen protein industry; Product yield reaches more than 90%, and its purity reaches more than 85%.
Embodiment
Below the preferred embodiments of the present invention are described in detail.
Rabbit → pre-treatment (clean, lose hair or feathers, shred) → except foreign protein → cleaning → acid soak, making beating → enzymolysis → centrifugal → lyophilize → finished product collagen protein of saltouing → wash
Embodiment 1
(1) pre-treatment: cleaned by fresh rabbit, immersing massfraction is spend the night in the NaCl solution of 10%, after removing subcutaneous lipids, by trichogen (to the Na of 5%
2s adds Ca (OH)
2to pasty state) room temperature is applied to subcutaneous, loses hair or feathers after 4h.Abundant washing is also cut into the fritter of about 3mm × 3mm, be placed in rabbit mass volume ratio that to be the massfraction of 1:5 be again 1% NaCl solution soak to remove foreign protein (soaking and stirring 6h, every 2h changes a not good liquor), with distilled water wash 3-4 time, vacuum packaging cold storage is for subsequent use.
(2) acid soak: be 1:15 by the mass volume ratio of rabbit and acetic acid, adds the acetum that pH value is 1.9, under the temperature condition of 3 DEG C, soaks 10h, and expanding evenly until skin bit breaks into pulpous state.
(3) enzymolysis: continuing to add pH value in the rabbit smashed is the acetum of 1.9, makes overall solution volume be 30 times of rabbit quality, stirs into uniform slurries, and slurries pH is controlled near 1.9.In rabbit quality, in rabbit slurries, add the stomach en-of 1%, enzymolysis 240min at 4 DEG C.
(4) saltout: filtered by enzymolysis solution, in filtrate, slowly drip NaOH pH to 7 ~ 8, more slowly add a certain amount of NaCl (making NaCl ultimate density be 4-5mol/L) while stirring, saltout 12h.
(5) wash centrifugal: thing of saltouing is centrifugal, taking precipitate, again washes centrifugal three times (5000r/min, 10min), until fully remove NaCl, gets precipitation, namely obtain collagen protein crude extract.
(6) freeze-drying: by the freeze-drying of collagen protein crude extract, obtains the collagen protein sample of purifying.
Embodiment 2
(1) pre-treatment: cleaned by fresh rabbit, immersing massfraction is spend the night in the NaCl solution of 10%, after removing subcutaneous lipids, by trichogen (to the Na of 5%
2s adds Ca (OH)
2to pasty state) room temperature is applied to subcutaneous, loses hair or feathers after 4h.Abundant washing is also cut into the fritter of about 3mm × 3mm, be placed in rabbit mass volume ratio that to be the massfraction of 1:5 be again 1% NaCl solution soak to remove foreign protein (soaking and stirring 6h, every 2h changes a not good liquor), with distilled water wash 3-4 time, vacuum packaging cold storage is for subsequent use.
(2) acid soak: be 1:15 by the mass volume ratio of rabbit and acetic acid, adds the acetum that pH value is 1.95, under the temperature condition of 4 DEG C, soaks 9h, and expanding evenly until skin bit breaks into pulpous state.
(3) enzymolysis: continuing to add pH value in the rabbit smashed is the acetum of 1.95, makes overall solution volume be 30 times of rabbit quality, stirs into uniform slurries, and slurries pH is controlled near 1.95.In rabbit quality, in rabbit slurries, add the stomach en-of 1%, enzymolysis 250min at 4 DEG C.
(4) saltout: filtered by enzymolysis solution, in filtrate, slowly drip NaOH pH to 7 ~ 8, more slowly add a certain amount of NaCl (making NaCl ultimate density be 4-5mol/L) while stirring, saltout 12h.
(5) wash centrifugal: thing of saltouing is centrifugal, taking precipitate, again washes centrifugal three times (5000r/min, 10min), until fully remove NaCl, gets precipitation, namely obtain collagen protein crude extract.
(6) freeze-drying: by the freeze-drying of collagen protein crude extract, obtains the collagen protein sample of purifying.
The rabbit collagen protein prepared by aforesaid method, its extraction yield is (94.17 ± 0.57) %, purity (85.57 ± 0.42) %, ash oontent is (0.873 ± 0.098) %, moisture content is (6.31 ± 0.43) %, and it reaches the index request of Collagen Hydrolysate (QB 2732-2005).Meanwhile, Product Process shortens the extraction time of collagen protein greatly, fundamentally improve the extraction long present situation consuming time of collagen protein industry; Product yield reaches more than 90%, and its purity reaches more than 85%.
What finally illustrate is, above preferred embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although by above preferred embodiment to invention has been detailed description, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from claims of the present invention limited range.
Claims (5)
1. from rabbit, extract a method for collagen protein, it is characterized in that, concrete steps are as follows:
(1) rabbit pre-treatment: rabbit is cleaned, except subcutaneous lipids, depilation, stripping and slicing, removes foreign protein, then wash;
(2) acid soak: get acetic acid mixing immersion 8 ~ 10h that rabbit that step (1) processed and pH value are 1.85 ~ 1.95, the mass volume ratio of described rabbit and acetic acid is 1:14 ~ 16;
(3) enzymolysis: the rabbit that step (2) processes is smashed in pulpous state, again continue add pH value be 1.85 ~ 1.95 acetic acid stir, the mass volume ratio of rabbit and acetic acid is 1:29 ~ 31, in rabbit quality, add the stomach en-of 0.095% ~ 1.005%, under 3 ~ 5 DEG C of temperature condition, enzymolysis 230-250min, filters to get filtrate;
(4) saltout: in filtrate, add NaOH regulate pH to 7 ~ 8, then to add NaCl to concentration be 4 ~ 5mol/L, carries out saltouing 12h;
(5) centrifugal: thing of saltouing is centrifugal, taking precipitate, then add water centrifugal until fully remove NaCl, get precipitation, namely obtain collagen protein crude extract;
(6) freeze-drying: by the freeze-drying of collagen protein crude extract, obtain the collagen protein of purifying.
2. from rabbit, extract the method for collagen protein according to claim 1, it is characterized in that: in described step (1) rabbit pre-treatment, rabbit is cut into 3mm × 3mm block.
3. from rabbit, extract the method for collagen protein according to claim 1, it is characterized in that, in described step (3) enzymolysis process, add pH value be 1.9 acetic acid stir, the mass volume ratio of rabbit and acetic acid is 1:30, in rabbit quality, add the stomach en-of 1%, enzymolysis 240min under 4 DEG C of temperature condition.
4. from rabbit, extract the method for collagen protein according to claim 1, it is characterized in that, step (1) described removal foreign protein mode is: be the NaCl solution mixing of 1% by rabbit and massfraction with mass volume ratio 1:4 ~ 6, soak 350 ~ 370min, every 120min changes a not good liquor.
5. from rabbit, extract the method for collagen protein according to claim 1, it is characterized in that: step (5) is each centrifugal 10min under rotating speed is 5000r/min condition.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929556A (en) * | 2017-04-12 | 2017-07-07 | 吉林省东鳌鹿业科技开发有限公司 | A kind of extracting method of sika deer deerskin collagen |
| CN107126579A (en) * | 2017-05-15 | 2017-09-05 | 中国科学院西北高原生物研究所 | A kind of former protein sponge of Yak-skin Gelatin acted on quick-acting haemostatic powder and its preparation method and application |
| CN107475337A (en) * | 2017-08-18 | 2017-12-15 | 合肥丰洁生物科技有限公司 | A kind of method that collagen is extracted in the sebum from animal |
| CN108991395A (en) * | 2018-09-07 | 2018-12-14 | 青海省畜牧兽医科学院 | The preparation method of yak meat products |
| CN109369799A (en) * | 2018-10-31 | 2019-02-22 | 白晋 | A kind of preparation method of soluble collagen freeze-drying tablet |
| CN109432499A (en) * | 2018-10-31 | 2019-03-08 | 白晋 | A kind of preparation method of the autologous collagen protein injection agent of combination secret reparation |
| CN109619375A (en) * | 2018-11-15 | 2019-04-16 | 青海省畜牧兽医科学院 | A kind of product and preparation method based on ox by-product |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2096467C1 (en) * | 1996-07-02 | 1997-11-20 | Татьяна Флавиановна Миронова | Method of treatment of rabbit hide |
-
2015
- 2015-06-29 CN CN201510365884.5A patent/CN104894202A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2096467C1 (en) * | 1996-07-02 | 1997-11-20 | Татьяна Флавиановна Миронова | Method of treatment of rabbit hide |
Non-Patent Citations (5)
| Title |
|---|
| 于玮 等: "猪皮胶原蛋白提取过程中酶解条件优化及其结构鉴定", 《西南大学学报（自然科学版）》 * |
| 冯文坡 等: "兔皮Ⅰ型胶原的提取、改性与性能研究", 《北京理工大学学报》 * |
| 康强 等: "《服装材料与试测技术》", 30 June 2014, 东华大学出版社 * |
| 成都科学技术大学 等: "《高等学校轻工业试用教材 制革化学及工艺学 上》", 30 June 1982, 轻工业出版社 * |
| 王鸿儒 等: "《皮革生产的理论与技术》", 31 July 1999, 中国轻工业出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929556A (en) * | 2017-04-12 | 2017-07-07 | 吉林省东鳌鹿业科技开发有限公司 | A kind of extracting method of sika deer deerskin collagen |
| CN107126579A (en) * | 2017-05-15 | 2017-09-05 | 中国科学院西北高原生物研究所 | A kind of former protein sponge of Yak-skin Gelatin acted on quick-acting haemostatic powder and its preparation method and application |
| CN107475337A (en) * | 2017-08-18 | 2017-12-15 | 合肥丰洁生物科技有限公司 | A kind of method that collagen is extracted in the sebum from animal |
| CN108991395A (en) * | 2018-09-07 | 2018-12-14 | 青海省畜牧兽医科学院 | The preparation method of yak meat products |
| CN109369799A (en) * | 2018-10-31 | 2019-02-22 | 白晋 | A kind of preparation method of soluble collagen freeze-drying tablet |
| CN109432499A (en) * | 2018-10-31 | 2019-03-08 | 白晋 | A kind of preparation method of the autologous collagen protein injection agent of combination secret reparation |
| CN113209380A (en) * | 2018-10-31 | 2021-08-06 | 白晋 | Preparation method of autologous collagen injection combined with privacy repair |
| CN109619375A (en) * | 2018-11-15 | 2019-04-16 | 青海省畜牧兽医科学院 | A kind of product and preparation method based on ox by-product |
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Application publication date: 20150909 |