CN101792382A - Method for preparing disinfected-decolorized fish-squamosum organic acid calcium and non-denatured collagen - Google Patents
Method for preparing disinfected-decolorized fish-squamosum organic acid calcium and non-denatured collagen Download PDFInfo
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- CN101792382A CN101792382A CN201010121325A CN201010121325A CN101792382A CN 101792382 A CN101792382 A CN 101792382A CN 201010121325 A CN201010121325 A CN 201010121325A CN 201010121325 A CN201010121325 A CN 201010121325A CN 101792382 A CN101792382 A CN 101792382A
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Abstract
The invention provides a method for preparing disinfected-decolorized fish-squamosum organic acid calcium and non-denatured collagen. The method comprises the following steps: adding disinfected decolorized fish squamosum solids to 10 to 20 percent organic acid in a solid-liquid ratio of 1:5-10; using energy-gathering ultrasonic wave to treat the obtained product for 4 to 24 hours; performing filtration and solid-liquid separation to obtain a crude product of collagen fish squamosum; standing filtrate at 10 DEG C for 4 to 15 hours to obtain organic acid calcium precipitate; filtering and drying the obtained product to obtain a finished product of disinfected-decolorized fish-squamosum organic acid calcium; adding 0.5 to 1 percent organic acid and/or 0.1 to 1 percent acidproof protease solution to the crude product of fish squamosum collagen in a solid-liquid ratio of 1:5-10; using energy-gathering ultrasonic wave to treat the obtained product at 10 to 35 DEG C for 2 to 4 hours until the crude product of fish squamosum collagen dissolves in the solution; removing impurities; and drying filtrate to obtain non-denatured collagen. The method has the advantages of low production cost and capability of obtaining disinfected-decolorized fish-squamosum organic acid calcium and non-denatured collagen at a time.
Description
Technical field:
The present invention is relevant with the preparation method of disinfected-decolorized fish-squamosum organic acid calcium, non-denatured collagen.
Background technology:
Fish scale bone calcium component is about 58%-62%, and protein accounts for 26%～55%, and bone calcium is formed near skeleton.Its year, consumption reached the hundreds of thousands of ton, and at present, supply falls short of demand.Protein component and human collagen in the fish scale bone (main components such as skin, cartilage) structural similitude, and a little less than the antigenicity, fabulous affinity is arranged and have good Bc with human body skin, itself and hyperplasia, differentiation, motion, immunity, joint lubrication, wound healing etc. are closely related, can be used as the upholder of tissue, pair cell, tissue repair have great effect.And can adhere to by induced platelet, activate blooc coagulation factor, hemostasis, and the migration of tool participation cell, differentiation and breeding improve bone tendon, skin physical strength, the effect of the repair tissue surface of a wound.The said products is in chemical industry, food, beauty treatment, field of medicaments widespread use.On the other hand, China is maximum in the world aquaculture state, 8287 families of existing processing of aquatic products enterprise, annual working ability reaches 1,306 ten thousand tons, and the waste total amount of annual fish just reaches more than 2,000,000 tons, special fresh-water fishes processing, the fish scale bone accounts for 50～70%, causes great environmental stress.
In recent years, the recycling of fish scale bone becomes focus, mainly with collagen protein extract, strong acid and strong base decalcification (deliming) is many, and is with direct disintegrating process with fish scale bone system calcium, all do not have the coproduction of disinfected-decolorized fish scale bone and prepare organic calcium, non-denatured collagen.
Summary of the invention:
The objective of the invention is in order to provide a kind of production cost low, can a coproduction obtain the preparation method of disinfected-decolorized fish-squamosum organic acid calcium, non-denatured collagen.
The object of the present invention is achieved like this:
The preparation method of disinfected-decolorized fish-squamosum organic acid calcium of the present invention, non-denatured collagen, this method is by solid-to-liquid ratio 1: 5～10, in concentration expressed in percentage by weight is to add disinfected-decolorized fish scale bone solid in 10～20% organic acids, and be interrupted or circular treatment 4～24 hours with the energy-collecting ultrasonic ripple, filtration, solid, liquid separate, obtain collagen protein fish scale bone crude product, filtrate was left standstill under 10 ℃ 4～15 hours, obtain the calcium of organic acid precipitation, filter, drying promptly makes the disinfected-decolorized fish-squamosum organic acid calcium finished product; By solid-to-liquid ratio 1: 5～10, adding concentration expressed in percentage by weight in fish scale bone collagen crude product is 0.5%～1% organic acid and/or 0.1～1% acid resistance protein enzyme solution, under 10～35 ℃, be interrupted or circular treatment 2～4 hours with the energy-collecting ultrasonic ripple, be dissolved in the solution up to fish scale bone collagen crude product, the removal of impurity, filtrate concentrate or directly spraying or lyophilize, promptly make non-denatured collagen.
Above-mentioned organic acid is lactic acid, citric acid, acetate, tartaric acid, thionamic acid.
Above-mentioned energy-collecting ultrasonic wave frequency is 10～60 kilo hertzs of sesames.
The method of the disinfected-decolorized fish scale bone of above-mentioned preparation is that tap water dioxide peroxide medicament and water are activated by weight dissolving in 8: 20, be made into concentration expressed in percentage by weight again and be 0.001～1.0% tap water ClO 2 solution, adding granularities by solid-to-liquid ratio 1: 5～10 is 2～5 millimeters fish-bone and defatted flakes, normal temperature (20～26 ℃) stirs down and soaked 1～24 hour, repeat at least 2 times, sterilization, decolouring is finished, select tap water dioxide peroxide medicament for use, disinfected-decolorizedly carry out simultaneously, dioxide peroxide is disinfected-decolorized in pre-treatment to carry out simultaneously, reduced production cost, disinfected-decolorized easily (a little less than adsorptive power), safety, the employing granularity was that 2～5 millimeters fish-bone and defatted flakes are for the proteic tissue of water miscible organic calcium and insoluble collagen is separated before fish-bone squama collagen protein extracted.
After above-mentioned disinfected-decolorized the finishing, wash 0～5 time, the degree of being with the odorlessness drains or controls and does or dry, and makes 40～60 order dry powder at last and gets final product.
In the above-mentioned method fish scale bone meal is broken into behind 20～60 orders normal temperature (20～60 ℃) add down repeat in the tap water ClO 2 solution at least 2 times disinfected-decolorized, and focus on extract before by at least 2 times disinfected-decolorized, reach the good purpose of fish scale collagen materials purification and commodity.
Adopt the energy-collecting ultrasonic ripple to make calcium break away from the constraint of histocyte structure by to effects such as the cavitation of osteocyte, vibrations in the inventive method, generate calcium of organic acid and combine with organic acid, calcium of organic acid can be widely used in food, the feed; With proteolytic enzyme and/or organic acid the collagen protein in the fish scale bone collagen crude product weave construction of decalcification is dissociated out; the energy-collecting ultrasonic ripple is to promote its free dissolved; because used organic collagen protein spirane structure destruction is little, hydrolysis temperature is lower than 35 ℃; make the fish scale bone collagen protect original three-dimensional spiral structure or strand peptide unchangeability, make that the product that obtains is a non-denatured collagen.
Preparation method of the present invention is simple, but coproduction obtains disinfected-decolorized fish-squamosum organic acid calcium, non-denatured collagen, safety, production efficiency height.
Embodiment:
Concentration expressed in percentage by weight is 10% tap water dioxide peroxide medicament, 100 grams, add 1000 milliliters of dissolving activation of water, after the mixed solution flavescence green, moisturizing to 100000 milliliter (content 0.01%) adds 10 kilograms of the fish-bone of 3 millimeters sizes and defatted flakes, and continuously stirring was soaked 2 hours under the normal temperature, can repeat 1～2 time, wash the degree of being with the odorlessness, disinfected-decolorized finishing several 0～1 time.Disinfected-decolorized fish scale bone solid, pressed solid-to-liquid ratio 1: 8, in concentration expressed in percentage by weight is to add disinfected-decolorized fish scale bone in 15% organic acid solution (as the lactic acid citric acid etc.), and is interrupted (or circulation) with the energy-collecting ultrasonic ripple and handled 10～15 hours, filtration, solid, liquid separates, obtain collagen protein fish scale bone crude product, filtrate is put and was left standstill under 10 ℃ 6～12 hours, obtains the calcium of organic acid precipitation, filtration, drying promptly make the disinfected-decolorized fish-squamosum organic acid calcium finished product; Pressed solid-to-liquid ratio 1: 8, adding concentration expressed in percentage by weight in fish scale bone collagen crude product is 0.75% organic acid (and/or 0.5% acid resistance proteolytic enzyme), under 10～35 ℃ of temperature, the energy-collecting ultrasonic ripple is interrupted (or circulation) to be handled 2～4 hours, repetition is dissolved up to major part, and spraying (or freezing) drying promptly makes non-denatured collagen.The energy-collecting ultrasonic wave frequency is 10～60 dried He Zhi.
The foregoing description is that foregoing of the present invention is further described, but this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to the foregoing description.All technology that realizes based on foregoing all belong to scope of the present invention.
Claims (6)
1. the preparation method of disinfected-decolorized fish-squamosum organic acid calcium, non-denatured collagen, this method is by solid-to-liquid ratio 1: 5～10, is to add disinfected-decolorized fish scale bone solid in 10～20% organic acids in concentration expressed in percentage by weight, and with energy-collecting ultrasonic ripple interruption or circular treatment 4～24 hours, filter, solid, liquid separates, and obtains collagen protein fish scale bone crude product, and filtrate was left standstill under 10 ℃ 4～15 hours, obtain the calcium of organic acid precipitation, filter, drying promptly makes the disinfected-decolorized fish-squamosum organic acid calcium finished product; By solid-to-liquid ratio 1: 5～10, adding concentration expressed in percentage by weight in fish scale bone collagen crude product is 0.5%～1% organic acid and/or 0.1～1% acid resistance protein enzyme solution, under 10～35 ℃, be interrupted or circular treatment 2～4 hours with the energy-collecting ultrasonic ripple, be dissolved in the solution up to fish scale bone collagen crude product, the removal of impurity, filtrate concentrate or directly spraying or lyophilize, promptly make non-denatured collagen.
2. the preparation method of disinfected-decolorized fish-squamosum organic acid calcium as claimed in claim 1, non-denatured collagen is characterized in that organic acid is lactic acid, citric acid, acetate, tartaric acid, thionamic acid.
3. the preparation method of disinfected-decolorized fish-squamosum organic acid calcium as claimed in claim 1, non-denatured collagen is characterized in that the energy-collecting ultrasonic wave frequency is 10～60 kilo hertzs of sesames.
4. as the preparation method of the described disinfected-decolorized fish-squamosum organic acid calcium of one of claim 1～3, non-denatured collagen, it is characterized in that preparing disinfected-decolorized fish scale bone is that tap water dioxide peroxide medicament and water are activated by weight dissolving in 8: 20, be made into concentration expressed in percentage by weight again and be 0.001～1.0% tap water ClO 2 solution, adding granularities by solid-to-liquid ratio 1: 5～10 is 2～5 millimeters fish-bone and defatted flakes, normal temperature stirs down and soaked 1～24 hour, repeat disinfected-decolorized finishing at least 2 times.
5. the preparation method of sterilization and decolorization of fish scale bones fish-squamosum organic acid calcium as claimed in claim 4, non-denatured collagen, after it is characterized in that disinfected-decolorized finishing, wash degree of being 0～5 time with the odorlessness, drain or control and do or dry, become 40～60 order dry powder to get final product at last.
6. the preparation method of sterilization and decolorization of fish scale bones fish-squamosum organic acid calcium as claimed in claim 4, non-denatured collagen, it is characterized in that fish scale bone meal is broken into behind 20～60 orders normal temperature to add in the tap water ClO 2 solution down and repeat at least 2 times, disinfected-decolorized.
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| CN201010121325A CN101792382A (en) | 2010-03-10 | 2010-03-10 | Method for preparing disinfected-decolorized fish-squamosum organic acid calcium and non-denatured collagen |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946907A (en) * | 2010-08-06 | 2011-01-19 | 海南大学 | Method for producing collagen calcium |
| CN102551111A (en) * | 2012-03-08 | 2012-07-11 | 天津工业大学 | Preparation method for fish bone organic acid calcium peptide powder |
| CN102972500A (en) * | 2012-11-27 | 2013-03-20 | 四川省农业科学院农产品加工研究所 | Method for removing calcium in fish scales |
| CN103202419A (en) * | 2012-01-15 | 2013-07-17 | 华中农业大学 | A compound fish scale peptide and calcium compound chewable tablet for antioxidation and calcium supplementing and a preparation method thereof |
| CN103539855A (en) * | 2013-09-18 | 2014-01-29 | 温州科技职业学院 | Extraction method of acaudina molpadioidea collagen |
| CN104004086A (en) * | 2013-10-17 | 2014-08-27 | 江苏江大五棵松生物科技有限公司 | Method for extracting fish scale collagen by using energy-gathered type pulsed ultrasound assistant hot water |
| CN105054132A (en) * | 2015-08-13 | 2015-11-18 | 江苏省农业科学院 | Fish scale jelly production process |
| CN109369800A (en) * | 2018-11-09 | 2019-02-22 | 贵州梵净山生态农业股份有限公司 | The extracting method of high-purity collagen in a kind of ox bone |
| CN111635920A (en) * | 2020-05-21 | 2020-09-08 | 内蒙古元本生物医药科技有限公司 | Method for preparing donkey bone collagen peptide powder by two-stage bionic enzymatic hydrolysis technology |
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| CN101307348A (en) * | 2008-06-13 | 2008-11-19 | 华中农业大学 | Method for preparing undenatured collagen from fish scale of fresh water fish |
| CN101586139A (en) * | 2009-06-25 | 2009-11-25 | 上海理工大学 | Method of extracting scale collagen polypeptide by microwave-assisted enzyme |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946907A (en) * | 2010-08-06 | 2011-01-19 | 海南大学 | Method for producing collagen calcium |
| CN103202419A (en) * | 2012-01-15 | 2013-07-17 | 华中农业大学 | A compound fish scale peptide and calcium compound chewable tablet for antioxidation and calcium supplementing and a preparation method thereof |
| CN103202419B (en) * | 2012-01-15 | 2014-07-16 | 华中农业大学 | A compound fish scale peptide and calcium compound chewable tablet for antioxidation and calcium supplementing and a preparation method thereof |
| CN102551111A (en) * | 2012-03-08 | 2012-07-11 | 天津工业大学 | Preparation method for fish bone organic acid calcium peptide powder |
| CN102972500A (en) * | 2012-11-27 | 2013-03-20 | 四川省农业科学院农产品加工研究所 | Method for removing calcium in fish scales |
| CN103539855B (en) * | 2013-09-18 | 2015-08-26 | 温州科技职业学院 | A kind of extracting method of acaudina molpadioidea collagen |
| CN103539855A (en) * | 2013-09-18 | 2014-01-29 | 温州科技职业学院 | Extraction method of acaudina molpadioidea collagen |
| CN104004086A (en) * | 2013-10-17 | 2014-08-27 | 江苏江大五棵松生物科技有限公司 | Method for extracting fish scale collagen by using energy-gathered type pulsed ultrasound assistant hot water |
| CN105054132A (en) * | 2015-08-13 | 2015-11-18 | 江苏省农业科学院 | Fish scale jelly production process |
| CN105054132B (en) * | 2015-08-13 | 2018-08-07 | 江苏省农业科学院 | A kind of production technology of fish scale jelly |
| CN109369800A (en) * | 2018-11-09 | 2019-02-22 | 贵州梵净山生态农业股份有限公司 | The extracting method of high-purity collagen in a kind of ox bone |
| CN109369800B (en) * | 2018-11-09 | 2021-12-21 | 贵州梵净山生态农业股份有限公司 | Method for extracting high-purity collagen from bovine bone |
| CN111635920A (en) * | 2020-05-21 | 2020-09-08 | 内蒙古元本生物医药科技有限公司 | Method for preparing donkey bone collagen peptide powder by two-stage bionic enzymatic hydrolysis technology |
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Address after: 610066 Sichuan Provincial Academy of Agricultural Sciences, Jinjiang District Province, Sichuan Province, Lion Hill Road, No. 4, Institute of agricultural products processing Applicant after: Song Yan Address before: 610100, No. 588, east route, Longquan Economic Development Zone, Chengdu, Sichuan Applicant before: Song Yan |
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Application publication date: 20100804 |