CN101744090A - Method for preparing small molecular peptides of soft-shelled turtle - Google Patents
Method for preparing small molecular peptides of soft-shelled turtle Download PDFInfo
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Abstract
The invention discloses a method for preparing small molecular peptides of soft-shelled turtle, belonging to the field of health product production technology. The method comprises the steps: pretreatment of fresh and live soft-shelled turtle as raw material; digestion by pancreatic enzyme-boiling and heat preservation-digestion by complex enzyme; degreasing by means of high-speed separation; decolorization and fishy smell removal by activated carbon; vacuum concentration; alcoholic precipitation and impurity removal; and ultrahigh temperature sterilizing, spray drying and the like. The method has the characteristics of advanced technology, being safe and healthy and excellent quality and efficiency, and the yield of peptides of soft-shelled turtle reaches 8-9%, wherein the small molecular peptides with the molecular weight below 5000 Daltons accounts for over 95%, and the content of protein in the product which is purely white and powdery is not less than 90%, thus the product accords with microbial health indexes and is liable to be absorbed and utilized by intestines and skins of human. The method can be popularized and applied to the enterprises of health products and beauty/skin care products.
Description
Technical field
The present invention relates to the production technical field of nutrient and healthcare products, be specifically related to utilize fresh and alive soft-shelled turtle to produce the manufacture method of small molecular peptides of soft-shelled turtle.
Background technology
Soft-shelled turtle claims soft-shelled turtle, the soft-shelled turtle again, is a kind of aquatic economic animal of preciousness, Fresh ﹠ Tender in Texture delicious, nutritious, contain protein 165g in every kilogram of meat, fat 10g, carbohydrate 16g, ash content 9g, calcium 1070mg, phosphorus 1350mg, iron 14mg, thiamine 6.2mg, riboflavin 3.7mg, niacin 37mg, the effect of have improving the health, promoting longevity.In addition, soft-shelled turtle also contains abundant Mg, Fe, Ca, Zn, Mn, the Se element, have enrich blood, strong bone, intelligence development, antifatigue, oxidation resistant effect.Because soft-shelled turtle has very high nutrition and medical value, is exactly traditional dietotherapy excellent tonic product of China since ancient times, Japan and Southeast Asia one band also are considered as the treasure that complement is kept fit to it, are the good raw materials of exploitation high-class healthy food.Soft-shelled turtle polypeptide is to be raw material with fresh and alive soft-shelled turtle, adopts biotechnology, through special enzymolysis, purification, separation, refining a kind of high-protein nutrient health care or the human body skin-protection product that forms.The soft-shelled turtle polypeptide of producing for the raw material hydrolysis with the soft-shelled turtle, the general equal wide spectrum of its molecular weight is distributed within the 100-20000 dalton scope, but the micromolecule polypeptide of molecular weight below 5000 dalton wherein, then have more and easily digested and assimilated or directly absorbed by skin institute by human body intestinal canal, wherein the micromolecule polypeptide of being digested and assimilated by enteron aisle is except keeping very high nutritive value and medical value, have also that stronger inhibition Angiotensin-Converting (ACE) is active to bring high blood pressure down or stop increased blood pressure, its antioxidation activity can delay senility, improve abilities such as immunity to reach; And the micromolecule polypeptide that is directly absorbed by skin is except that providing abundant nutrition, more to skin have tangible skin care wrinkle resistant, protect the function of moistening; In addition, the following micromolecule polypeptide of 5000 dalton also can avoid producing problems such as precipitation, emulsification are bad, clarity reduction when being further processed into nutrient and healthcare products or skin-protection product.
In recent years, though the relevant report that adopts enzyme process to produce soft-shelled turtle polypeptide is arranged, as enzymatic liquefaction and the health instant powder research of (Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology) soft-shelled turtles such as Duan Xuchang, Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology's journal in April, 2003 (the 31st the 2nd phase of volume); The technical research of Xu Huaide etc. (Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology) soft-shelled turtle enzymatic liquefaction, Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology's journal in October, 2003 (the 31st the 5th phase of volume); Soft-shelled turtles such as Li Zhicheng are soaked juice and the research of soft-shelled turtle enzymolysis liquid fermentation technique, Chinese food journal in June, 2004 (the 4th the 2nd phase of volume); The research of happy heavily fortified point etc. (Pingxiang, Jiangxi Province higher junior college) enzymatic hydrolysis turtle proteins, food industry 1997 06 phases of science and technology.Because all only simple method for hydrolysis of using enzyme process or fermentation method of above-mentioned technology, its technology simply causes enzymolysis not thorough, and the product yield is low; Simultaneously, said method is not made separation, the purification processes of micromolecule polypeptide in process of production to product; In addition, the technologies such as vacuum drying that adopted are carried out post-production, have length consuming time, and efficient is low, cost height, defectives such as shade deviation.Therefore, do not see systematic report as yet for the manufacture method that adopts fresh and alive soft-shelled turtle to produce micromolecule polypeptide.
Summary of the invention
The present invention seeks to, defectives such as, product yield low, consuming time length simple, efficient is low and product color is poor, sized molecules amount polypeptide mixes at existing technology in the existing soft-shelled turtle polypeptide production technology, a kind of technology advanced person, supporting is proposed, product protein content 〉=90%, pure white, powdery, yield is up to 8-9%, and wherein polypeptide molecular weight accounts for the manufacture method of the small molecular peptides of soft-shelled turtle more than 95% below 5000 dalton.
The object of the invention is achieved by the following technical programs:
Small molecular peptides of soft-shelled turtle of the present invention is meant the soft-shelled turtle polypeptide of its molecular weight below 5000 dalton.
A kind of manufacture method of small molecular peptides of soft-shelled turtle, this method is carried out according to the following steps:
(1) pretreatment of raw material: to select fresh and alive soft-shelled turtle for use be raw material through slaughtering, striping, go internal organ, chopping, ultralow temperature to pulverize the back and drop in the reactor, and add entry in fresh and alive soft-shelled turtle raw material weight (raw material weight of following each step all is meant the weight of fresh and alive soft-shelled turtle) and 1: 1 ratio of water weight; Be heated to 80 ℃ of insulations and be cooled to 60 ℃ after half an hour, standby:
(2) pancreatin enzymolysis-boil insulation-complex enzyme zymohydrolysis is handled: press the 0.05-0.1% adding pancreatin of raw material weight, under temperature 55-60 ℃, pH value 6.5-7.0 enzymolysis 1-4 hour; Be heated to boil after, 90-95 ℃ of down insulation 10 hours; After being cooled to 60 ℃, the 0.05-0.1% that presses raw material weight adds compound protease, under temperature 55-60 ℃, pH value 6.5-7.0 enzymolysis 1-4 hour; Emit enzymolysis liquid after the end, get coarse filtration liquid, waste with the sub-coarse filtration of 100 mesh sieves earlier; Again coarse filtration liquid is put into reactor, add 160 purpose crushed crude pearlites, emit after stirring, be heated to 80 ℃ of half an hour, filter to get filtrate with the deckle board filter press by the 4-8% of raw material weight;
(3) high-speed separation degrease: the high speed butterfly chip seperator of filtrate with 10000 rev/mins separated, goes to get clear liquid 1 behind the free oil of clean upper strata;
(4) activated carbon decolorizing, take off raw meat: clear liquid 1 is put into Decolouring pot, and the 1-3% that presses raw material weight adds active carbon, stirs to be heated to 85-90 ℃ down and to decolour, take off raw meat and handle after 30 minutes, emits while hot and with the filtration of deckle board filter press, the destainer that must clarify;
(5) vacuum concentrates: destainer is put into vacuum decker, be evaporated to the concentrate that peptide concentration is 30-40% under 60 ℃;
(6) alcohol precipitation, impurity elimination are handled: concentrate is sent into stirred in the reactor when being cooled to below 50 ℃, stirred 10 minutes add 95% edible alcohol by 10% of this liquid weight after, filter with plate filter after static again 2-4 hour, remove 5000 dalton above big molecular polypeptide and contamination precipitation thing, get clear liquid 2;
(7) UHTS and spray-drying: is 135-140 ℃ with clear liquid 2 employing moment superhigh temperature disinfecting machines at sterilising temp, and the outlet feed temperature is to sterilize under 75-80 ℃; Sterilized solution being sent into the storage of aseptic basin, progressively send into then in the high speed moment spray dryer, is 200-220 ℃ in inlet temperature, outlet temperature be spray under 80-100 ℃ dried, white powdery small molecular peptides of soft-shelled turtle finished product.
Described complex enzyme by 1392 type neutral proteinases and pancreatin by weight 2: 1 ratios mix.
Beneficial effect of the present invention:
The one, adopt the multiple method for hydrolysis of the pancreatin enzymolysis complex enzyme zymohydrolysis that---boils insulation---: the top layer collagen of at first using single pancreatin enzymolysis soft-shelled turtle raw material, it is bulk the abundant slaking of meat layer azelon of raw material inside to boil insulation again, at last with the meat azelon and the collagen of the abundant enzymolysis of complex enzyme inside, thereby make turtle proteins obtain sufficient hydrolysis, by 6.5% of conventional method soft-shelled turtle polypeptide (including the above big molecular polypeptide of 5000 dalton) yield, bring up to the 8-9% of the inventive method small molecular peptides of soft-shelled turtle yield, both significantly improved the yield of polypeptide, and improved the quality of polypeptide again and (seen embodiment 3,4).
The 2nd, adopt high speed separator to separate a large amount of greases of removing in the enzymolysis: make the activated carbon decolorizing deodorization effect improve, the rate of filtration is accelerated, thereby activated carbon dosage is reduced, and has improved the yield of polypeptide.
The 3rd, adopt alcohol precipitation to remove big molecular polypeptide and impurity: because the general equal wide spectrum of molecular weight of gained hydrolyzed peptide liquid is distributed within the 100-20000 dalton scope behind the enzymolysis, big molecular polypeptide more than 5000 dalton is adopted the method that reaches precipitation in 10% the solution, removes at 95% edible alcohol content, both safety and sanitation, convenient and swift again, do not influence the inherent quality of product.
The 4th, adopt the sterilization of superhigh temperature instantaneously sterilising machine: polypeptide liquid is boiled under the condition at 100 ℃ usually can not kill bacillus, adopt superhigh temperature instantaneously sterilising machine not only can kill bacillus but also can not influence product quality in time second at 135 ℃ of high temperature 5-10, and shortened sterilization time, improved sterilizing efficiency.
The 5th, adopted high speed moment spray to do technology and carried out drying: changed traditional low temperature drying and brought the shortcoming that efficient is low, investment is big, cost is high, improve product quality, guaranteed product microbiological indicator (removing the pollution that traditional finished product radiation sterilizing brings from) up to standard.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail, but following description does not constitute the qualification of going up in all senses to protection scope of the present invention.
Pancreatin: 4000u/g, the biochemical factory in the Qujiang River, Sichuan produces;
Neutral proteinase: 1392 types, enzyme preparation factory in Wuxi produces;
Active carbon: 303 types, Quzhou cloud Jiang Huoxingtanchang produces;
Crushed crude pearlite: 160 orders, Correspondent industrial corporation in Xinyang produces;
High speed butterfly chip seperator: the DHZ400 type, Shenyang Machinery Plant of Light Industry produces;
Decolouring pot: 1000L type stainless steel cauldron, Shangyu connection Feng Yalirongqichang produces;
Vacuum decker: evaporation capacity 300kg/h, Wenzhou grand light industry and machinery of profit Manufacturing Co., Ltd produces;
Plate filter: the WPG-2 type, Wenzhou Long Wan Xing Aoguan steel filter plant factory produces;
Moment superhigh temperature disinfecting machine: 1000kg/h, the sharp grand light industry and machinery in Wenzhou Manufacturing Co., Ltd produces;
High speed moment spray dryer: the QZ50 type, the dried machine of the big spray of no cylinder factory produces;
Hand-held saccharometer: WYT-4 type, Quanzhou Zhongyou's optical instrument Co., Ltd;
Super-low temperature pulverizator: size reduction machinery Co., Ltd of DFZ type Jiangyin City gold section;
Embodiment 1:(small molecular peptides of soft-shelled turtle preparation 1)
Carry out according to the following steps:
(1) pretreatment of raw material: select for use fresh and alive soft-shelled turtle raw material 400kg through slaughter, striping, go internal organ, chopping, input super-low temperature pulverizator to be cooled to-170 ℃ of pulverizing after, drop in the reactor again, and add water 400kg in fresh and alive soft-shelled turtle raw material weight (raw material weight of following each step all is meant the weight of fresh and alive soft-shelled turtle) and 1: 1 ratio of water weight; Be heated to 80 ℃ of insulations and be cooled to 60 ℃ after half an hour, standby;
(2) pancreatin enzymolysis-boil insulation-complex enzyme hydrolysis is handled: press 0.05% of raw material weight and add pancreatin 200g, temperature 55-60 ℃, with 10%NaOH liquid accent pH value to 6.5-7.0, enzymolysis 4 hours; Be heated to boil after, 90-95 ℃ of down insulation 10 hours; After being cooled to 60 ℃, pressing 0.1% of raw material weight and add compound protease 400g, enzymolysis is 1 hour under temperature 55-60 ℃, pH value 6.5-7.0; Enzymolysis is emitted enzymolysis liquid after finishing, and gets coarse filtration liquid, waste with the sub-coarse filtration of 100 mesh sieves earlier; Again this coarse filtration liquid is put into reactor, press 8% of raw material weight and add crushed crude pearlite 32kg, stir and be heated to 80 ℃, emit after half an hour, filter to get filtrate with the deckle board filter press;
(3) high-speed separation degrease: filtrate is separated, removed its upper strata free oil and get clear liquid 1 with 10000 rev/mins high speed butterfly chip seperator;
(4) activated carbon decolorizing, take off raw meat: clear liquid 1 is put into Decolouring pot, stirs and press 1% of raw material weight down and add active carbon 4kg, be heated to 85-90 ℃ and decolour, take off raw meat and handle after 30 minutes, emit while hot and with the filtration of deckle board filter press, the destainer that must clarify;
(5) vacuum concentrates: destainer is put into vacuum decker, the control temperature 60 ℃ down decompression concentrate, and to increase to hand-held saccharometer measurement peptide concentration be 30%, gets concentrate 120kg;
(6) alcohol precipitation, impurity elimination are handled: concentrate is cooled to below 50 ℃, and stirred 10 minutes behind 10% adding, the 95% edible alcohol 12kg by this concentrate weight, quiescent setting filtered with plate filter after 2 hours again, remove 5000 dalton above big molecular polypeptide and contamination precipitation thing, get clear liquid 2;
(7) UHTS and spray-drying: step (6) clear liquid 2 is adopted the moment superhigh temperature disinfecting machine, and the control temperature is 135-140 ℃, and the outlet feed temperature is to sterilize under 75-80 ℃; Sterilized solution is sent into the storage of aseptic basin, progressively sends into high speed moment spray dryer then, and inlet temperature is controlled at 200-220 ℃, outlet temperature be controlled at spray under 80-100 ℃ dried, white powdery small molecular peptides of soft-shelled turtle finished product 32kg, yield is 8%.
Embodiment 2:(small molecular peptides of soft-shelled turtle preparation 2)
In this example, the fresh and alive soft-shelled turtle raw material selected for use of step (1) is 400kg; Step (2) is pressed 0.075% of raw material weight and is added pancreatin 300g, enzymolysis 2.5 hours; Press 0.075% of raw material weight in addition and add compound protease 300g, enzymolysis 2.5 hours; Press 6% of raw material weight again and add crushed crude pearlite 24kg; Step (4) is pressed 2% of raw material weight and is added active carbon 8kg; It is 35% concentrate 110kg that step (5) is evaporated to peptide concentration with destainer; Step (6) is pressed 10% of concentrate weight and is added 95% edible alcohol 11kg, quiescent setting 3 hours; Step (7) gets white powdery small molecular peptides of soft-shelled turtle finished product 34kg after UHTS and spray-drying, yield is 8.5%; All the other steps, technology are same as embodiment 1.
Embodiment 3:(small molecular peptides of soft-shelled turtle preparation 3)
In this example, the fresh and alive soft-shelled turtle raw material selected for use of step (1) is 400kg; Step (2) is pressed 0.1% of raw material weight and is added pancreatin 400g, enzymolysis 1 hour; Press 0.05% of raw material weight in addition and add compound protease 200g, enzymolysis 4 hours; Press 4% of raw material weight again and add crushed crude pearlite 16kg; Step (4) is pressed 3% of raw material weight and is added active carbon 12kg; It is 40% concentrate 100kg that step (5) is evaporated to peptide concentration with destainer; Step (6) is pressed 10% of concentrate weight and is added 95% edible alcohol 10kg, quiescent setting 4 hours; Step (7) gets white powdery small molecular peptides of soft-shelled turtle finished product 36kg after UHTS and spray-drying, yield is 9%; All the other steps, technology are same as embodiment 1.
Embodiment 4:(adopts conventional method to produce soft-shelled turtle polypeptide)
(1) after fresh and alive soft-shelled turtle raw material 400kg slaughters striping and internal organ chopping, drops into reactor and add the 400kg pure water again, be heated to 80 ℃ and be incubated half an hour, be cooled to 60 ℃, add pancreatin 400g, enzymolysis 4 hours, 55-60 ℃ of temperature of control, pH value 6.0-6.5 (adjusting) with 10%NaOH liquid;
(2) enzymolysis finishes, and is heated to the enzyme that goes out 85-90 ℃ of half an hour;
(3) go out enzymolysis liquid, with the sub-coarse filtration of 100 mesh sieves, waste, coarse filtration liquid is put into reactor and is added the 32kg crushed crude pearlite, be heated with stirring to 80 ℃, emit after half an hour, filter with the deckle board filter press;
(4) the gained clear liquid is put into Decolouring pot, under agitation adds the 20kg activated carbon decolorizing, takes off the raw meat processing, and treatment temperature 85-90 ℃, half an hour in processing time, processing finishes to emit while hot with the deckle board filter press filters the destainer that must clarify;
(5) destainer concentrates through vacuum, is concentrated into peptide concentration and is 35% concentrate 85kg, and the temperature that decompression concentrates is controlled at below 60 ℃;
(6) the dense filtrate of gained is adopted moment superhigh temperature disinfecting machine sterilization, 135-140 ℃ of control sterilising temp, and 75-80 ℃ of outlet feed temperature, the gained sterilized solution is sent into aseptic basin;
(7) sterilization filtrate adopts the spray of high speed moment spray dryer to do, and inlet temperature is controlled at 200-220 ℃, and outlet temperature is controlled at 80-100 ℃, gets powdery finished product 26kg, and yield is 6.5%.
Table 1 example 4 compares in the distribution of polypeptide yield and different molecular weight with example 3
Claims (2)
1. the manufacture method of a small molecular peptides of soft-shelled turtle is characterized in that this method carries out according to the following steps:
(1) pretreatment of raw material: to select fresh and alive soft-shelled turtle for use be raw material through slaughtering, striping, go internal organ, chopping, ultralow temperature to pulverize in the input reactor of back, and add entry in fresh and alive soft-shelled turtle raw material weight and 1: 1 ratio of water weight; Be heated to 80 ℃ of insulations and be cooled to 60 ℃ after half an hour, standby:
(2) pancreatin enzymolysis-boil insulation-complex enzyme zymohydrolysis is handled: press the 0.05-0.1% adding pancreatin of raw material weight, under temperature 55-60 ℃, pH value 6.5-7.0 enzymolysis 1-4 hour; Be heated to boil after, 90-95 ℃ of down insulation 10 hours; After being cooled to 60 ℃, the 0.05-0.1% that presses raw material weight adds compound protease, under temperature 55-60 ℃, pH value 6.5-7.0 enzymolysis 1-4 hour; Emit enzymolysis liquid after the end, get coarse filtration liquid, waste with the sub-coarse filtration of 100 mesh sieves earlier; Again coarse filtration liquid is put into reactor, add 160 purpose crushed crude pearlites, emit after stirring, be heated to 80 ℃ of half an hour, filter to get filtrate with the deckle board filter press by the 4-8% of raw material weight;
(3) high-speed separation degrease: the high speed butterfly chip seperator of filtrate with 10000 rev/mins separated, goes to get clear liquid 1 behind the free oil of clean upper strata;
(4) activated carbon decolorizing, take off raw meat: clear liquid 1 is put into Decolouring pot, and the 1-3% that presses raw material weight adds active carbon, stirs to be heated to 85-90 ℃ down and to decolour, take off raw meat and handle after 30 minutes, emits while hot and with the filtration of deckle board filter press, the destainer that must clarify;
(5) vacuum concentrates: destainer is put into vacuum decker, be evaporated to the concentrate that peptide concentration is 30-40% under 60 ℃;
(6) alcohol precipitation, impurity elimination are handled: concentrate is sent into stirred in the reactor when being cooled to below 50 ℃, stirred 10 minutes add 95% edible alcohol by 10% of this liquid weight after, filter with plate filter after static again 2-4 hour, remove 5000 dalton above big molecular polypeptide and contamination precipitation thing, get clear liquid 2;
(7) UHTS and spray-drying: is 135-140 ℃ with clear liquid 2 employing moment superhigh temperature disinfecting machines at sterilising temp, and the outlet feed temperature is to sterilize under 75-80 ℃; Sterilized solution being sent into the storage of aseptic basin, progressively send into then in the high speed moment spray dryer, is 200-220 ℃ in inlet temperature, outlet temperature be spray under 80-100 ℃ dried, white powdery small molecular peptides of soft-shelled turtle finished product.
2. by the described manufacture method of claim 1, it is characterized in that described complex enzyme by 1392 type neutral proteinases and pancreatin by weight 2: 1 ratios mix.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286588A (en) * | 2011-08-18 | 2011-12-21 | 江中药业股份有限公司 | Method for preparing turtle peptide |
| CN104611398A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Oyster peptide extraction method |
| CN104611399A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Method for extracting sea cucumber polypeptide |
| CN105238545A (en) * | 2015-09-23 | 2016-01-13 | 集美大学 | Preparation method for combined production of micromolecular soft-shelled turtle peptide and soft-shelled turtle oil |
| CN105341767A (en) * | 2015-12-09 | 2016-02-24 | 哈尔滨神守生物科技有限公司 | Preparing method for soft-shelled turtle protein peptide |
| CN106343146A (en) * | 2016-08-23 | 2017-01-25 | 慈晓丽 | Preparation method of turtle peptide |
| CN106954587A (en) * | 2017-04-24 | 2017-07-18 | 蚌埠农兴达现代农业有限公司 | A kind of broiler feeding method |
| CN107095800A (en) * | 2017-05-04 | 2017-08-29 | 安徽万甲宴食品科技有限公司 | Composition and preparation method containing turtle peptide and the facial mask containing said composition |
| CN108251484A (en) * | 2018-03-21 | 2018-07-06 | 杭州早稻田生物技术有限公司 | Soft-shelled turtle active peptide albumen powder and preparation method thereof |
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2010
- 2010-01-25 CN CN2010101031579A patent/CN101744090B/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102286588A (en) * | 2011-08-18 | 2011-12-21 | 江中药业股份有限公司 | Method for preparing turtle peptide |
| CN102286588B (en) * | 2011-08-18 | 2015-04-22 | 江中药业股份有限公司 | Method for preparing turtle peptide |
| CN104611398A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Oyster peptide extraction method |
| CN104611399A (en) * | 2015-01-27 | 2015-05-13 | 张恒 | Method for extracting sea cucumber polypeptide |
| CN105238545A (en) * | 2015-09-23 | 2016-01-13 | 集美大学 | Preparation method for combined production of micromolecular soft-shelled turtle peptide and soft-shelled turtle oil |
| CN105341767A (en) * | 2015-12-09 | 2016-02-24 | 哈尔滨神守生物科技有限公司 | Preparing method for soft-shelled turtle protein peptide |
| CN106343146A (en) * | 2016-08-23 | 2017-01-25 | 慈晓丽 | Preparation method of turtle peptide |
| CN106954587A (en) * | 2017-04-24 | 2017-07-18 | 蚌埠农兴达现代农业有限公司 | A kind of broiler feeding method |
| CN107095800A (en) * | 2017-05-04 | 2017-08-29 | 安徽万甲宴食品科技有限公司 | Composition and preparation method containing turtle peptide and the facial mask containing said composition |
| CN108251484A (en) * | 2018-03-21 | 2018-07-06 | 杭州早稻田生物技术有限公司 | Soft-shelled turtle active peptide albumen powder and preparation method thereof |
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|---|---|
| CN101744090B (en) | 2012-02-22 |
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Effective date of registration: 20221009 Address after: Floor 1-2, North of the West Building, Huihua Industrial Plant, District 7, Tongxin Community, Jianggan District, Hangzhou City, Zhejiang Province, 311500 Patentee after: Hangzhou Zhongde Peptide Biological Products Co.,Ltd. Address before: No. 287-1, Airport Road, Hangzhou, Zhejiang 310021 Patentee before: HANGZHOU ZHONGDE HEALTHY FOOD Co.,Ltd. |
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