CN103348845A - Method for planting good-quality and high-yield oilseed rape - Google Patents
Method for planting good-quality and high-yield oilseed rape Download PDFInfo
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- CN103348845A CN103348845A CN201310249563XA CN201310249563A CN103348845A CN 103348845 A CN103348845 A CN 103348845A CN 201310249563X A CN201310249563X A CN 201310249563XA CN 201310249563 A CN201310249563 A CN 201310249563A CN 103348845 A CN103348845 A CN 103348845A
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Abstract
The invention discloses a method for planting good-quality and high-yield oilseed rape. During an oilseed rape growing period, a nutrient is applied; and the nutrient mainly comprises components in percentage by weight as follows: 10%-80% of nucleotide salt and deoxynucleotide salt and 20%-90% of sugar or/and amino acid. During the growing period, the time when the nutrient is applied and the usage amount are as follows: the nutrient is sprayed for two times, the first time is a blossom stage, 20 grams of the nutrient is mixed with 20 liters of water, and then the mixture is sprayed on the oilseed rape of one mu; and the second time is a full-bloom stage, 20 grams of the nutrient is mixed with 30 liters of water, and the mixture is sprayed on the oilseed rape of one mu. The method has the advantages as follows: the nutrient is non-poisonous, harmless, and residue-free to the oilseed rape and avoids adverse side effects; the production is increased, the efficiency is improved, the resistance is enhanced, and the effect for improving the quality is good; and the method is simple, the cost is low, and large-scale production and popularization are facilitated.
Description
The application is application number 201010514217.6, the applying date: on October 21st, 2010, denomination of invention is divided an application for " nutrient of a kind of Promoting plant growth and preparation method thereof ".
Technical field
The present invention relates to the good quality and high output implantation methods of a kind of implantation methods of rape, particularly a kind of rape.
Background technology
Greatly develop plant husbandry, forestry, promote that crop yield increases income, make our country green, it is the problem that the various countries scientist pays close attention to research, the growth of plant be unable to do without to supplement the nutrients, and present plant husbandry is mainly by executing N,P,K fertilizers and organic manure promotes crop growth, it is very large on the quality and flavor impact of crops to use too much fertilizer, is unfavorable for development and food security and the environmental protection of green agriculture.Therefore, people have studied relevant growth hormone with nucleic acid, nutrient, fertilizer.In theory: nucleic acid is the basic substance that consists of life, is the basis of metabolism and energy metabolism, and the character of nucleic acid and the difference of quantity can cause the diversity of biological species and growth conditions.For congener life entity, the quantity of nucleic acid and the difference of existence form have determined strong and weak prosperous the declining of this life entity.Therefore, nucleic acid substances has irreplaceable effect for the output increased in crops and even the production of whole vegetative kingdom and product quality improvement; Particularly several nucleotides salt and deoxyribonucleoside hydrochlorate and the cooperations thereof of the little molecule of nucleic acid such as nucleosides, nucleotide, pyrimidine and purine, the not only very effectively growth of regulating plant, can be used as again nutrient source is absorbed rapidly by plant, strengthen the plant root vigor, improve the plant anti-adversity ability, thus improve crop output, improve product quality.The little molecule of nucleic acid has the enhancing improving activity of root system to plant, improves root to the absorption of phosphorus, potash fertilizer; Improve the activity of crops malate dehydrogenase and succinic acid enzyme, promote the assimilational capacity of plant, strengthen photosynthetic intensity, to plant growth have regulate and trophism (Wang Xi etc. the application and function research on mechanism [J] of nucleic acid degradation product on Rice Production. Acta Biochimica et Biophysica Sinica, 1975,7 (1): 31-39).3., the hydrolysis of nucleic acid 2., dissolving ribonucleic acid (alkali lye hydrotropy) 1., the lixiviate nuclease (publication number: 101186534) disclose a kind of preparation method who contains the nucleic acid degradation product foliage fertilizer, the concrete step is Chinese patent literature:,,, must contain
The little molecule of multiple nucleic acids and a small amount of reducing sugar and few big or middle, trace elements such as nucleosides, nucleotide, purine, pyrimidine are arranged, the method is take ribonucleic acid as raw material, with enzymatic hydrolysis, exist production cost high, it is not thorough to degrade, and seldom contains the deoxynucleotide composition, and nucleotide salt yield is low in the mixed system, the mutual mixing ratio imbalance of various nucleosides hydrochlorates and deoxynucleotide salt face is very large on the result of use impact.Chinese patent literature (publication number: 101050143) disclose a kind of nutrient that promotes crop growth, it is comprised of Ribavirin and glucose, production and the guanosine of Ribavirin are in close relations, and there is the Ribavirin high cost in this patent, the unconspicuous problem of practical effect; Chinese patent literature (publication number: 1238323) disclose a kind of preparation method of nucleic acid foliage fertilizer, the method of alkaline hydrolysis is adopted in the degraded of its nucleic acid, degradation product is purine and pyrimidine, there is the nucleic acid high cost equally in this patent, various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are unreasonable, affect the problem of result of use.
Summary of the invention
The purpose of this invention is to provide a kind of good quality and high output implantation methods of rape, its production cost is low, and method is simple, is easy to large-scale production and popularization; Nucleotide salt yield is high in the mixed system, and reasonable, nontoxic, harmless, the noresidue of various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face is without any adverse side effect; Increase production, increase income, synergy, strengthen resistance, it is effective to improve quality;
The present invention is achieved in that
The good quality and high output implantation methods of rape: during growth of rape, use nutrient; Described nutrient mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or/and amino acid forms, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, sugar is or/and amino acid 20-90%; Time and usage amount that described growing period is used nutrient are: spraying twice, is initial bloom stage for the first time, be watered 20 liters with 20 gram nutrients and spray on one mu of rape, and be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape.
The good quality and high output implantation methods of rape: the sugar in the described nutrient is glucose or sucrose.
The nutrient of Promoting plant growth, it mainly is comprised of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, auxiliary agent, and its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, auxiliary agent 20-90%.
The nutrient of Promoting plant growth, in nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, percentage by weight is: nucleosides hydrochlorate 85-99%, deoxyribonucleoside hydrochlorate 1-15%.
The nutrient of Promoting plant growth, in nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, percentage by weight is: nucleosides hydrochlorate 88-96%, deoxyribonucleoside hydrochlorate 4-12%.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and auxiliary agent, percentage by weight is:
Nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-30%, auxiliary agent 70-90%.
The nutrient of Promoting plant growth, auxiliary agent are sugar or amino acid.
The nutrient of Promoting plant growth, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are sodium salt or sylvite.
The preparation method of the nutrient of Promoting plant growth provided by the present invention, concrete steps are as follows: take nucleic acid as raw material, mainly be that to adopt the nucleic acid of dry method or wet production be raw material for mycelium after the fermentation plant fermentation:
1., the hydrolysis of nucleic acid is the ratio mixed nucleus bronsted lowry acids and bases bronsted lowry liquid of 1:20-30 according to nucleic acid, buck weight ratio, alkali lye is 5.5-7N, under 30-45 ℃ of condition, stirred 10-14 hours, and got nucleosides hydrochlorate and deoxyribonucleoside acid salt solution, filter, filtrate is transferred pH8-8.5, reduced pressure concentration;
2., decolouring, dry concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 10-25%;
3., nucleosides hydrochlorate and the deoxyribonucleoside hydrochlorate of crystallization after doing with clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, gets white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt.
4., nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and water soluble mixt are pulverized, cross 200 mesh sieves and mix with auxiliary agent again.
In the described nucleic acid hydrolysis process of this preparation method, used alkali lye is sodium hydroxide or potassium hydroxide solution.
In the present invention, the nucleosides hydrochlorate mainly refers to: 5 '-adenylate disodium or potassium, 5 '-bird adenylate disodium or potassium, 5 '-cytidylic acid disodium or potassium, 5 '-four kind 5 in uridine monophosphate disodium or potassium '-sodium salt or the sylvite of mixed nucleotides; The deoxyribonucleoside hydrochlorate refers to: 5 '-desoxyadenossine acid disodium or potassium, 5 '-deoxidation bird adenylate disodium or potassium, 5 '-deoxycytidine acid disodium or potassium, 5 '-the BrdU acid disodium,, 5 '-five kind 5 in deoxyribosylthymine acid disodium or potassium '-sodium salt or the sylvite of deoxidation mixed nucleotides.The source of nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate is for take nucleic acid as raw material, mainly be mycelium after the fermentation plant fermentation to adopt the nucleic acid of dry method or wet production be raw material, this kind nucleic acid mixes a small amount of deoxidation nuclear take ribonucleic acid (RNA) as main
Ribosomal ribonucleic acid (DNA) is made with the alkali lye hydrolysis; Auxiliary agent is mainly derived from two aspects: 1, the nucleic acid of dry method or wet production also contains the impurity such as sugar, protein, various metals or nonmetallic ion, and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc. are arranged after the hydrolysis; 2, exogenous adding: sugar (reducing sugar, non reducing sugar: also have fructose etc. such as glucose, sucrose), amino acid, big or middle, micro-etc.
The present invention adopt mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, it is take nucleic acid as raw material that the Promoting plant growth nutrient that auxiliary agent forms reaches, mainly be from fermentation plant (Gourmet Powder Factory, brewery, antibiotics plant, yeast factory, Amino Acid Factory, the paper mill, activated sludge after the waste water treatment, petro-jelly fermentations etc.) nucleic acid of the employing dry method of by product (offcuts) mycelium after the fermentation or wet production is the preparation method of raw material hydrolysis, with existing product and the method (product that adds Ribavirin, the product that directly is hydrolyzed with ribonucleic acid, enzyme solution) compares and have the following advantages: 1, its production cost of mycelium production ribonucleic acid after the fermentation plant fermentation is low, is easy to large-scale production and popularization; 2, the nucleic acid of mycelium production contains useful impurity, in addition basic hydrolysis method, technique is simple, nucleotide salt yield is high in the mixed system, and various nucleosides hydrochlorates and the mutual mixing ratio of deoxynucleotide salt face are reasonable, and amino acid, sugar part and other organic-inorganic auxiliary agent content are higher, therefore, increasing both production and income synergy strengthens resistance, and it is effective to improve quality; 3,, nontoxic, harmless, noresidue, without any adverse side effect; 4, be widely used in grain, cotton, oil, cigarette, tea, really, plant and the bacterium mushroom series products such as dish, traditional Chinese medicine, flowers, forest.
Embodiment
Embodiment 1: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, and glucose 6 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 2: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, and sucrose 2 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 3: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, glucose 8 grams, nucleosides hydrochlorate and taking off
Oxygen nucleosides hydrochlorate is pulverized, and mixes with glucose, namely gets nutrient.
Embodiment 4: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, and glucose 8.5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 5: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1 gram, and glucose 9 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 6: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2.5 grams, and glucose 7.5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 7: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 4 grams, and fructose 6 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 8: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 5 grams, and glucose 5 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate are pulverized, and mix with glucose, namely get nutrient.
Embodiment 9: get nucleotide potassium and deoxynucleotide potassium 1 gram, and glucose 9 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 10: get nucleotide potassium and deoxynucleotide potassium 1.5 grams, and glucose 8.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 11: get nucleotide potassium and deoxynucleotide potassium 2 grams, and glucose 8 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 12: get nucleotide potassium and deoxynucleotide potassium 2.5 grams, and glucose 7.5 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 13: get nucleotide potassium and deoxynucleotide potassium 3 grams, and glucose 7 grams, nucleotide potassium and deoxynucleotide potassium are pulverized, and mix with glucose, namely get nutrient.
Embodiment 14: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 1.5 grams, and glucose 4.5 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient.
Embodiment 15: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, and glucose 4 grams, amino acid 4 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient.
Embodiment 16: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 3 grams, and glucose 4 grams, amino acid 3 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix with glucose, namely get nutrient.
Embodiment 17: get nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 2 grams, and amino acid 8 grams, nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and amino acid are pulverized, and mix, and namely get nutrient.
Embodiment 18: crude product ribonucleic acid (RNA) 100 grams that will make from the mycelium of fermentation plant's by product with dry method or wet method, be dissolved in the sodium hydroxide solution of 3 liters of 6.3N, under 37 ℃ of conditions, stirred 12 hours, the winner will refer to contain: 5 '-the adenylate disodium, 5 '-bird adenylate disodium, 5 '-the cytidylic acid disodium, 5 '-four kind 5 of uridine monophosphate disodium '-sodium salt of mixed nucleotides (also have the deoxyribonucleoside hydrochlorate: 5 '-the desoxyadenossine acid disodium, 5 '-deoxidation bird adenylate disodium, 5 '-the deoxycytidine acid disodium, 5 '-the BrdU acid disodium, 5 '-five kind 5 of deoxyribosylthymine acid disodium '-sodium salt and the amino acid of deoxidation mixed nucleotides, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, is evaporated to an amount of; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into moisture 15%; With nearly new-nucleo and the deoxyribonucleotide natrium of doing of proper amount of clear water dissolving, its solution adds alcohol and separates out, the crystallization suction filtration is dry, get approximately 85-90 grams, white nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate and a certain amount of helping and the agent water soluble mixt, gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (when again adding auxiliary agent).
Embodiment 19: crude product ribonucleic acid (RNA) 100 grams that will make from the mycelium of fermentation plant's by product with dry method or wet method, be dissolved in the potassium hydroxide solution of 2.5 liter of 6 N, under 40 ℃ of conditions, stirred 10 hours, the winner will refer to contain: 5 '-the adenylate dipotassium, 5 '-bird adenylate dipotassium, 5 '-the cytidylic acid dipotassium, 5 '-four kind 5 of uridylic acid dipotassium '-sylvite of mixed nucleotides (also have the deoxyribonucleoside hydrochlorate: 5 '-the deoxyadenylic acid dipotassium, 5 '-deoxidation bird adenylate dipotassium, 5 '-the deoxycytidylic acid dipotassium, 5 '-
The deoxyuridylic acid dipotassium,, 5 '-five kind 5 of deoxythymidylic acid dipotassium '-sylvite of deoxidation mixed nucleotides and amino acid, sugar part and nitrogen, phosphorus, potassium, iron, calcium, magnesium, zinc, copper, manganese etc.) solution, filter, filtrate is neutralized to pH8-8.5, is evaporated to an amount of; Concentrate is added activated carbon decolorizing, suction filtration it, filtrate decompression is concentrated into 20%; With nearly nucleotide potassium and the deoxynucleotide potassium of doing of proper amount of clear water dissolving, its solution adds alcohol separates out, and the crystallization suction filtration is dry, gets approximately 88-93 white nucleotide sylvite of gram and deoxyribonucleoside acid potassium salt and a certain amount of auxiliary agent and water soluble mixts.Gains are pulverized, crossed 200 mesh sieves, mix with auxiliary agent (when again adding auxiliary agent).
Embodiment 20: paddy rice: spray twice, for initial bloom stage (heading about 10%) is watered 30 liters with 20 gram nutrients (containing glucose 80-85%'s), about concentration 100ppm, evenly spray on one mu of paddy rice for the first time.Be full-bloom stage for the second time, after spraying for the first time, be watered 30 liters with 20 gram nutrients in 5-6 days, evenly spray in one mu of paddy rice and get final product (mu increase production 70-100 kilograms, increase production about 15%).
Embodiment 21: cotton: spray twice, for the first time also can initial bloom stage in the flower bud phase, and restrain nutrients with 30 and be watered 30 liters, evenly spray on one mu of cotton.For the second time full-bloom stage is watered 36 liters with 30 grams and evenly sprays in one mu of cotton and get final product (mu increase production more than 7%).
Embodiment 22: rape: spraying twice, is initial bloom stage for the first time, is watered 20 liters with 20 gram nutrients and sprays on one mu of rape.Be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape and get final product volume increase 15-20 kilogram.
Embodiment 23: tobacco: spray twice, with 30 grams, whenever be watered 30 kilograms, about concentration 200ppm at every turn, spray on one mu of tobacco leaf, spray and carry out 20-26 day in time in May, (the south north time is slightly different) sprays for the first time, 3-6 day in June carries out spraying the second time, about concentration 150ppm, quality improves a grade, volume increase about 20%.
Embodiment 24: tealeaves: at the growth germination period, spray for the first time, spray once after every harvesting, about concentration 180ppm.
Embodiment 25: capsicum: initial bloom stage and full-bloom stage each once, concentration 200-300ppm, mu volume increase 40%～80%.
Embodiment 26: strawberry: spray in the fruitlet phase, be watered 18 liters with 10 grams and spray, spray 3～4 times after the harvesting in batches and can increase production 38%.
Embodiment 27: traditional Chinese medicine: the traditional Chinese medicine of different parts, using method differs, generally initial bloom stage and full-bloom stage each once, concentration 100-260ppm.
Embodiment 28: mushroom: sprayed once in per 3 days from fruiting, and concentration 100-150ppm, collecting time 1/3rd is shortened in volume increase 50-80%.
Claims (2)
1. the good quality and high output implantation methods of rape is characterized in that: during growth of rape, use nutrient; Described nutrient mainly by nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate, sugar or/and amino acid forms, its percentage by weight is: nucleosides hydrochlorate and deoxyribonucleoside hydrochlorate 10-80%, sugar is or/and amino acid 20-90%; Time and usage amount that described growing period is used nutrient are: spraying twice, is initial bloom stage for the first time, be watered 20 liters with 20 gram nutrients and spray on one mu of rape, and be full-bloom stage for the second time, be watered 30 liters with 20 grams, spray on one mu of rape.
2. the good quality and high output implantation methods of described rape according to claim 1, it is characterized in that: the sugar in the described nutrient is glucose or sucrose.
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Cited By (3)
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| CN105152779A (en) * | 2013-11-19 | 2015-12-16 | 安徽天之正生物科技有限公司 | Nutrient for oilseed rape growth and use method of nutrient |
| CN105152758A (en) * | 2013-11-19 | 2015-12-16 | 合肥凯歌信息科技有限公司 | Nutrient for promoting rape growth and preparation method of nutrient |
| CN105152757A (en) * | 2013-11-19 | 2015-12-16 | 合肥凯歌信息科技有限公司 | Production method of rape and application of nutrient of rape |
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Address after: 230001 Luyang, Anhui Province, Taoyuan Road, No. 66, No. 107, China Railway Park, room, room 3 Applicant after: Hefei Kaige Information Technology Co., Ltd. Address before: Ma On Shan Road in Baohe District of Hefei city of Anhui province Berlin 230001 Spring District No. 16 101-106 Applicant before: Hefei Kaige Information Technology Co., Ltd. |
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Inventor after: Chen Fengxiang Inventor after: Feng Jiansen Inventor after: Guo Haihui Inventor after: Ru Linyun Inventor after: Zhou Fengxia Inventor after: Chen Fenge Inventor before: Gao Jing |
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Effective date of registration: 20171221 Address after: East Ring Road Suzhou District 735000 Gansu city of Jiuquan province No. 19 Patentee after: Jiuquan Lan Xiang gardening and seedling Co., Ltd. Address before: 230001 Luyang, Anhui Province, Taoyuan Road, No. 66, No. 107, China Railway Park, room, room 3 Patentee before: Hefei Kaige Information Technology Co., Ltd. |