CN103348017B - 使用检测剂、探针和抑制剂检测核酸靶标 - Google Patents

使用检测剂、探针和抑制剂检测核酸靶标 Download PDF

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Publication number
CN103348017B
CN103348017B CN201180058333.4A CN201180058333A CN103348017B CN 103348017 B CN103348017 B CN 103348017B CN 201180058333 A CN201180058333 A CN 201180058333A CN 103348017 B CN103348017 B CN 103348017B
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China
Prior art keywords
nucleic acid
inhibitor
target nucleic
fluorophore
detection agent
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Expired - Fee Related
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CN201180058333.4A
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English (en)
Chinese (zh)
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CN103348017A (zh
Inventor
T·拉兹
P·玛丽
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Bio Rad Laboratories Inc
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Nubeo GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Dispersion Chemistry (AREA)
CN201180058333.4A 2010-12-07 2011-12-07 使用检测剂、探针和抑制剂检测核酸靶标 Expired - Fee Related CN103348017B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610045852.1A CN105603071B (zh) 2010-12-07 2011-12-07 使用检测剂、探针和抑制剂检测核酸靶标

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42074710P 2010-12-07 2010-12-07
US61/420,747 2010-12-07
PCT/US2011/063654 WO2012078710A1 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201610045852.1A Division CN105603071B (zh) 2010-12-07 2011-12-07 使用检测剂、探针和抑制剂检测核酸靶标

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CN103348017A CN103348017A (zh) 2013-10-09
CN103348017B true CN103348017B (zh) 2016-01-20

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CN201610045852.1A Expired - Fee Related CN105603071B (zh) 2010-12-07 2011-12-07 使用检测剂、探针和抑制剂检测核酸靶标

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Country Status (8)

Country Link
US (2) US9581549B2 (https=)
EP (1) EP2649202B1 (https=)
JP (1) JP6096672B2 (https=)
CN (2) CN103348017B (https=)
AU (1) AU2011338502B2 (https=)
CA (1) CA2820094C (https=)
SG (1) SG191725A1 (https=)
WO (1) WO2012078710A1 (https=)

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EP2691676B1 (en) 2011-03-30 2019-03-27 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
WO2012135327A1 (en) 2011-03-31 2012-10-04 Gnubio Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
HK1218151A1 (zh) 2013-01-25 2017-02-03 Bio-Rad Laboratories, Inc. 实施液滴膨胀的系统和方法
CN105408495B (zh) 2013-04-26 2019-01-08 基纽拜奥股份有限公司 通过茎环结构阻断3’dna末端的聚合酶延伸的方法
US9766261B2 (en) 2013-05-29 2017-09-19 Bio-Rad Laboratories, Inc. Low cost optical high speed discrete measurement system
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Publication number Publication date
US20130344485A1 (en) 2013-12-26
AU2011338502B2 (en) 2016-08-11
US9581549B2 (en) 2017-02-28
AU2011338502A1 (en) 2013-06-13
JP2014500729A (ja) 2014-01-16
CA2820094A1 (en) 2012-06-14
CN105603071A (zh) 2016-05-25
US10533212B2 (en) 2020-01-14
CA2820094C (en) 2019-02-26
CN103348017A (zh) 2013-10-09
EP2649202A1 (en) 2013-10-16
JP6096672B2 (ja) 2017-03-15
US20170226568A1 (en) 2017-08-10
EP2649202B1 (en) 2019-02-20
CN105603071B (zh) 2019-06-21
WO2012078710A1 (en) 2012-06-14
EP2649202A4 (en) 2015-05-27
SG191725A1 (en) 2013-08-30

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