CN103336117B - 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 - Google Patents
一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 Download PDFInfo
- Publication number
- CN103336117B CN103336117B CN201310251399.6A CN201310251399A CN103336117B CN 103336117 B CN103336117 B CN 103336117B CN 201310251399 A CN201310251399 A CN 201310251399A CN 103336117 B CN103336117 B CN 103336117B
- Authority
- CN
- China
- Prior art keywords
- detection method
- add
- antibody
- biotin
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 42
- 241001113283 Respirovirus Species 0.000 title claims abstract description 17
- 238000002965 ELISA Methods 0.000 title claims abstract 4
- 238000000034 method Methods 0.000 claims abstract description 29
- 230000003612 virological effect Effects 0.000 claims description 23
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 11
- 229960002685 biotin Drugs 0.000 claims description 10
- 235000020958 biotin Nutrition 0.000 claims description 10
- 239000011616 biotin Substances 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 9
- 108090001008 Avidin Proteins 0.000 claims description 8
- 238000013016 damping Methods 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical group C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 claims description 4
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 244000309466 calf Species 0.000 claims description 4
- 239000003593 chromogenic compound Substances 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 claims description 2
- 239000013642 negative control Substances 0.000 claims description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 2
- 230000009385 viral infection Effects 0.000 abstract description 13
- 238000003757 reverse transcription PCR Methods 0.000 abstract description 10
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 230000000241 respiratory effect Effects 0.000 abstract description 7
- 239000000427 antigen Substances 0.000 abstract description 6
- 102000036639 antigens Human genes 0.000 abstract description 6
- 108091007433 antigens Proteins 0.000 abstract description 6
- 230000003321 amplification Effects 0.000 abstract description 4
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 4
- 238000013399 early diagnosis Methods 0.000 abstract description 3
- 241000700605 Viruses Species 0.000 description 24
- 210000004027 cell Anatomy 0.000 description 14
- 241000712461 unidentified influenza virus Species 0.000 description 12
- 241000725643 Respiratory syncytial virus Species 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- CACCWEPNYHGKGD-UFLZEWODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid;n'-hydroxybutanediamide Chemical compound NC(=O)CCC(=O)NO.N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 CACCWEPNYHGKGD-UFLZEWODSA-N 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 206010022000 influenza Diseases 0.000 description 4
- 241000709661 Enterovirus Species 0.000 description 3
- 241000197306 H1N1 subtype Species 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 208000036142 Viral infection Diseases 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 238000012797 qualification Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 208000035473 Communicable disease Diseases 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 241000710799 Rubella virus Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000003547 immunosorbent Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000124740 Bocaparvovirus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 241000711573 Coronaviridae Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010017918 Gastroenteritis viral Diseases 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 201000008197 Laryngitis Diseases 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
- 241000351643 Metapneumovirus Species 0.000 description 1
- 241000711386 Mumps virus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 208000009525 Myocarditis Diseases 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 231100000645 Reed–Muench method Toxicity 0.000 description 1
- 241000606651 Rickettsiales Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 206010044302 Tracheitis Diseases 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 230000003097 anti-respiratory effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 208000015994 miscarriage Diseases 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 208000008423 pleurisy Diseases 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000012772 sequence design Methods 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 206010044008 tonsillitis Diseases 0.000 description 1
- 208000025301 tympanitis Diseases 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 239000005723 virus inoculator Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/11—Orthomyxoviridae, e.g. influenza virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/115—Paramyxoviridae, e.g. parainfluenza virus
- G01N2333/135—Respiratory syncytial virus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
稀释倍数 | 180 | 360 | 720 | 1440 | 2880 | 5760 | 11520 | 23040 | 46080 | 92160 |
OD值 | 2.85 | 2.82 | 2.79 | 2.67 | 2.0 | 1.42 | 0.75 | 0.33 | 0.19 | 0.12 |
稀释倍数 | 100 | 200 | 400 | 800 | 1600 | 3200 | 6400 | 12800 | 25600 | 51200 |
OD值 | 3.0 | 3.0 | 3.0 | 2.82 | 2.34 | 1.30 | 0.68 | 0.30 | 0.14 | 0.08 |
稀释倍数 | 180 | 360 | 720 | 1440 | 2880 | 5760 | 11520 | 23040 | 46080 | 92160 |
OD值 | 2.67 | 2.25 | 1.7 | 0.8 | 0.41 | 0.25 | 0.17 | 0.09 |
稀释倍数 | 100 | 200 | 400 | 800 | 1600 | 3200 | 6400 | 12800 | 25600 | 51200 |
OD值 | 2.71 | 2.25 | 1.24 | 0.51 | 0.33 | 0.18 | 0.06 |
Claims (6)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310251399.6A CN103336117B (zh) | 2013-06-13 | 2013-06-13 | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 |
PCT/CN2014/079608 WO2014198216A1 (zh) | 2013-06-13 | 2014-06-10 | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310251399.6A CN103336117B (zh) | 2013-06-13 | 2013-06-13 | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103336117A CN103336117A (zh) | 2013-10-02 |
CN103336117B true CN103336117B (zh) | 2015-10-21 |
Family
ID=49244321
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310251399.6A Active CN103336117B (zh) | 2013-06-13 | 2013-06-13 | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN103336117B (zh) |
WO (1) | WO2014198216A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103336117B (zh) * | 2013-06-13 | 2015-10-21 | 广州华银医药科技有限公司 | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 |
CN108872586A (zh) * | 2018-05-31 | 2018-11-23 | 佛山普文森生物科技有限公司 | 一种定量检测srf的酶联免疫分析方法 |
CN109557321A (zh) * | 2018-11-30 | 2019-04-02 | 江苏省农业科学院 | 一种鹅催乳素的双抗体夹心elisa检测方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101498724A (zh) * | 2009-01-24 | 2009-08-05 | 中国检验检疫科学研究院 | 玉米细菌性萎蔫病菌生物素-亲和素酶联免疫检测方法 |
CN101975858A (zh) * | 2010-10-15 | 2011-02-16 | 河北省农林科学院植物保护研究所 | 棉花黄萎菌酶联免疫检测试剂盒及其应用 |
CN102023217A (zh) * | 2010-12-01 | 2011-04-20 | 扬州大学 | 牛病毒性腹泻病毒双抗夹心生物素-亲和素elisa检测试剂盒及使用方法 |
CN102854317A (zh) * | 2011-09-27 | 2013-01-02 | 上海博沃生物科技有限公司 | 一种ev71抗原酶联反应检测试剂盒及其制备方法 |
CN103048459B (zh) * | 2012-12-05 | 2014-08-20 | 中国科学院武汉病毒研究所 | 检测呼吸道合胞病毒的免疫检测试剂 |
CN103336117B (zh) * | 2013-06-13 | 2015-10-21 | 广州华银医药科技有限公司 | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 |
-
2013
- 2013-06-13 CN CN201310251399.6A patent/CN103336117B/zh active Active
-
2014
- 2014-06-10 WO PCT/CN2014/079608 patent/WO2014198216A1/zh active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2014198216A1 (zh) | 2014-12-18 |
CN103336117A (zh) | 2013-10-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Najjar et al. | A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma | |
De Conto et al. | Epidemiology of human respiratory viruses in children with acute respiratory tract infection in a 3-year hospital-based survey in Northern Italy | |
Al Johani et al. | MERS-CoV diagnosis: an update | |
Ziegler et al. | Type-and subtype-specific detection of influenza viruses in clinical specimens by rapid culture assay | |
CN106596933B (zh) | 新城疫病毒抗体检测阻断elisa试剂盒 | |
Zhang et al. | SARS-CoV-2 detection using quantum dot fluorescence immunochromatography combined with isothermal amplification and CRISPR/Cas13a | |
Dilnessa et al. | Cell culture, cytopathic effect and immunofluorescence diagnosis of viral infection | |
Wang et al. | Simultaneous and automated detection of influenza A virus hemagglutinin H7 and H9 based on magnetism and size mediated microfluidic chip | |
Grudlewska-Buda et al. | SARS-CoV-2—morphology, transmission and diagnosis during pandemic, review with element of meta-analysis | |
CN103336117B (zh) | 一种呼吸道病毒的生物素-亲和素酶联免疫检测方法 | |
WO2021169664A1 (zh) | 新型冠状病毒抗原及其检测用途 | |
TW201300421A (zh) | 用於偵測豬繁殖及呼吸道綜合症病毒(prrsv)之試劑及方法 | |
CN104726606B (zh) | 一种pcr酶联双杂交法检测病原微生物检测方法 | |
Hollý et al. | Comparison of infectious influenza A virus quantification methods employing immuno-staining | |
Kodihalli et al. | Antigen-capture enzyme immunoassay for detection of avian influenza virus in turkeys | |
Chen et al. | A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009 | |
CN102495208A (zh) | 禽流感病毒rt-pcr elisa诊断方法及应用该方法的试剂盒 | |
Nguyen et al. | Rapid and broad detection of H5 hemagglutinin by an immunochromatographic kit using novel monoclonal antibody against highly pathogenic avian influenza virus belonging to the genetic clade 2.3. 4.4 | |
CN106589014A (zh) | 唾液酸寡糖‑磁纳米粒子及其制备方法与应用 | |
Alnaeem et al. | Clinical observations and molecular detection of Type-A influenza virus in some of the family Equidae in eastern Saudi Arabia winter-2019 | |
CN1176000A (zh) | 抗体生产的检测 | |
Al-Hajjar | Laboratory diagnosis of viral disease | |
Zhao et al. | Tetraplex fluorescent microbead-based immunoassay for the serodiagnosis of Newcastle disease virus and avian influenza viruses in poultry sera | |
Makkoch et al. | Determination of antibody response to the human pandemic influenza H1N1 2009 among patients with influenza-like illness and high risk groups | |
CN104797934B (zh) | 流感病毒的血凝素的测定方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Li Chuanjiang Inventor after: Zhu Bing Inventor after: Gong Lili Inventor before: Li Chuanjiang |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LI CHUANJIANG TO: LI CHUANJIANG ZHU BING GONG LILI |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee | ||
CP01 | Change in the name or title of a patent holder |
Address after: 510663, Guangdong Science City, Guangzhou science and technology innovation building innovation base, C District, 3 floor Patentee after: GUANGDONG HUAYIN MEDICINE TECHNOLOGY CO.,LTD. Address before: 510663, Guangdong Science City, Guangzhou science and technology innovation building innovation base, C District, 3 floor Patentee before: GUANGZHOU HUAYIN MEDICINE SCIENCE AND TECHNOLOGY Co.,Ltd. |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A biotin avidin enzyme linked immunosorbent assay for detection of respiratory viruses Effective date of registration: 20201127 Granted publication date: 20151021 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Panyu branch Pledgor: GUANGDONG HUAYIN MEDICINE TECHNOLOGY Co.,Ltd. Registration number: Y2020440000374 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20151021 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Panyu branch Pledgor: GUANGDONG HUAYIN MEDICINE TECHNOLOGY CO.,LTD. Registration number: Y2020440000374 |