CN103319360A - Process for extracting macamides from maca - Google Patents

Abstract

The invention provides a process for extracting macamides from maca. The method comprises the following steps of: a, extracting, namely, taking maca raw material, adding water which is 8-10 times of the maca raw material by weight, performing heating reflux extraction for three times at 70-85 DEG C, each for 40-60 minutes, and blending the water extracts of two times; b, adding ethanol for precipitation, namely, adding the ethanol which is two times of the maca raw material by weight to the water extract and precipitating for 24-48 hours, and then collecting the supernatant while abandoning the precipitate; c, carrying out vacuum concentrating, namely, concentrating the supernatant until each 1 ml of extract contains 1 g of raw material, thereby obtaining a concentrated solution; and d, purifying, namely, adding absolute ethyl alcohol to the concentrated solution, stirring, placing, performing decompression filtration to obtain precipitate, and then recrystallizing the precipitate by using the absolute ethyl alcohol, thereby obtaining macamides in the content greater than or equal to 98%. The process provided by the invention has the characteristics of simple technique, convenience for operation, short production period, cost saving, high yield and the like, and thereby is especially suitable for large-batch industrial production.

Description

From the agate coffee, extract the processing method of agate coffee acid amides

Technical field

The present invention relates to a kind of Effective Component of Chinese Medicine extracting and purifying method, specifically a kind of processing method of from the agate coffee, extracting agate coffee acid amides.

Background technology

Agate coffee (Maca), have another name called Peruvian ginseng, maka, maca-maca, maino, ayak chichira, ayak willku etc., originate in the mountain area, Andean, South America of 3500～4500 meters of height above sea level, now mainly be distributed in Puno ecotope and Peru the southeastern city of Puno at Peru middle part, be Cruciferae (Cruciferae) Lepidium (Lepidium) plant.That the agate coffee is divided into is white, yellow, purple, black four kinds, color is more deeply felt and is shown that quality is better, purple agate coffee is comparatively rare, and black is more precious rare.

The generally attention that the agate coffee obtains people is in the early 1990s, and the investigator has found this kind of plant in the remarkable efficacy that improves on the sexual function when seeking " vigour " substitute, makes the agate coffee become at one stroke a nova in international health product and the medicine; And before this, when the Spaniard when capturing South America in 1526, colonists just are used for solving the sterile difficult problem of people and animals to the necessary article that the agate coffee is taken root the mountain area, Andean as the procreation of living, and so far still as having the tribute that improves energy, fecundity effect and good tonic effect, be widely used by Spain imperial family.The agate coffee has had more than 5800 year in the edible history in South America in fact, is used for traditionally strengthening body, improves fertility, improves sexual function, antidepressant, anti-anaemia etc.From phase early 1960s to the eighties, progressively systematize of research about agate coffee phytology and pharmaceutical use, some traditional role of agate coffee obtain Germany and North America phytology investigator's scientific validation, especially after the eighties, Food and Argriculture OrganizationFAO (FAO) promotes the suggestion countries in the world plantation to the agate coffee, the chemical composition of agate coffee is identified, activeconstituents separation and pharmacological action thereof etc. have obtained further discussion, and wherein the agate coffee improves fertility, improve sexual function, anticancer, leukemia, the effects such as treatment climacteric syndrome become current study hotspot.

Summary of the invention

It is simple that purpose of the present invention just provides a kind of technique, easy handling, and yield is high, is suitable for the large processing method of extracting agate coffee acid amides from the agate coffee of producing of industry.

The present invention includes following step:

1. extract: get agate coffee raw material, add its weight 8-10 water doubly, in 70-85 ℃ of lower heating and refluxing extraction 3 times, each 40-60 minute, merge the intermediate water extracting solution;

2. add the ethanol precipitation: the ethanol that adds 2 times of amounts of agate coffee raw material weight in aqueous extract precipitates 24-48 hour, discards throw out, collects supernatant liquor;

3. vacuum concentration: supernatant concentration to every ml extracting solution is contained the lg crude drug, get concentrated solution;

4. purifying: in above-mentioned concentrated solution, add its weight 10-15 dehydrated alcohol doubly, transfer PH9-11 with sodium hydroxide solution again, the perlite that adds 0.5-1%, stir, filter, filtrate is transferred PH5-7 with hydrochloric acid soln again, place, decompress filter gets throw out, and throw out is used the dehydrated alcohol recrystallization again, namely makes the agate coffee acid amides of Han Liang＞=98%.

The inventive method has following characteristics:

1. technique is simple, and is with short production cycle, and the agate coffee raw material that the present invention selects extracts without pulverizing directly, has reduced pulverizing process, easy handling;

2. use the perlite consumption few, only use when recrystallization, cost is low.

Embodiment

Embodiment 1

1. extract: get agate coffee raw material, add its weight 8-10 water doubly, in 70-85 ℃ of lower heating and refluxing extraction 3 times, each 40-60 minute, merge the intermediate water extracting solution;

2. add the ethanol precipitation: the ethanol that adds 2 times of amounts of agate coffee raw material weight in aqueous extract precipitates 24-48 hour, discards throw out, collects supernatant liquor;

3. vacuum concentration: supernatant concentration to every ml extracting solution is contained the lg crude drug, get concentrated solution;

4. purifying: in above-mentioned concentrated solution, add its weight 10-15 dehydrated alcohol doubly, transfer PH9-11 with sodium hydroxide solution again, the perlite that adds 0.5-1%, stir, filter, filtrate is transferred PH5-7 with hydrochloric acid soln again, place, decompress filter gets throw out, and throw out is used the dehydrated alcohol recrystallization again, namely makes the agate coffee acid amides of Han Liang＞=98%.

Claims (1)

1. from the agate coffee, extract the processing method of agate coffee acid amides, it is characterized in that comprising the steps: successively

1. extract: get agate coffee raw material, add its weight 8-10 water doubly, in 70-85 ℃ of lower heating and refluxing extraction 3 times, each 40-60 minute, merge the intermediate water extracting solution;

2. add the ethanol precipitation: the ethanol that adds 2 times of amounts of agate coffee raw material weight in aqueous extract precipitates 24-48 hour, discards throw out, collects supernatant liquor;

3. vacuum concentration: supernatant concentration to every ml extracting solution is contained the lg crude drug, get concentrated solution;

4. purifying: in above-mentioned concentrated solution, add its weight 10-15 dehydrated alcohol doubly, transfer PH9-11 with sodium hydroxide solution again, the perlite that adds 0.5-1%, stir, filter, filtrate is transferred PH5-7 with hydrochloric acid soln again, place, decompress filter gets throw out, and throw out is used the dehydrated alcohol recrystallization again, namely makes the agate coffee acid amides of Han Liang＞=98%.