CN106176847A - A kind of have Quinoa saponin strengthening bacteriostasis and preparation method thereof - Google Patents
A kind of have Quinoa saponin strengthening bacteriostasis and preparation method thereof Download PDFInfo
- Publication number
- CN106176847A CN106176847A CN201610529182.0A CN201610529182A CN106176847A CN 106176847 A CN106176847 A CN 106176847A CN 201610529182 A CN201610529182 A CN 201610529182A CN 106176847 A CN106176847 A CN 106176847A
- Authority
- CN
- China
- Prior art keywords
- quinoa
- saponin
- preparation
- bacteriostasis
- quinoa saponin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of Quinoa saponin with enhancing bacteriostasis and preparation method and application.This preparation method, comprises the following steps: Quinoa wheat bran is pulverized 70% methanol extraction, filtering and concentrating extracting solution, ethyl acetate and n-butyl alcohol remove impurity, aqueous slkali modification, desalination, purification by macroporous resin, and last lyophilization obtains Quinoa saponin.The present invention can prepare the Quinoa saponin with enhancing fungistatic effect quickly and efficiently.By antibacterial culturing it is demonstrated experimentally that alkali process after the more non-alkali of Quinoa saponin process saponin fungistatic effect be obviously enhanced.Particularly through three kinds of oral cavity pathogens (porphyromonas gingivalis, perfringens Fusobacterium, Fusobacterium nucleatum) In vitro culture it is demonstrated experimentally that alkali process after the more non-alkali of Quinoa saponin process Quinoa saponin bacteriostasis be remarkably reinforced.
Description
Technical field
The present invention relates to extractive technique field, particularly to a kind of Quinoa saponin with enhancing bacteriostasis and preparation thereof
Method and application.
Background technology
Quinoa is a kind of cereal crops originating in South America, has the highest nutritive value.In recent years, China mountain
The ground such as west, Jilin, Qinghai, Gansu, Hebei all have extensive Quinoa to plant.Quinoa would generally during being processed into Quinoa rice
Produce the Quinoa wheat bran of about 20%-30%.Quinoa wheat bran contains the saponin constituent of about 2%-4%, causes Quinoa wheat bran taste
Pained, it is impossible to utilize directly as animal feed.Composition in research Quinoa wheat bran, makes full use of Quinoa wheat bran resource,
Turn waste into wealth, significant economic worth can be produced.
Halitosis is often as the oral cavity pathogen sulfide that raised growth breeding produces in oral cavity and causes.According to
China's stomatology can be added up, and China there are about 1.3 hundred million halitosis patients, therefore, has that to prevent and treat the product demand of halitosis effect vigorous.
Currently, with respect to the research of saponin component in Quinoa wheat bran it has been reported that as Quinoa saponin has immunomodulating and subtracts
Fertile activity, but relevant Quinoa saponin bacteriostasis, especially the research aspect of suppression oral cavity bacterium effect there is not yet relevant report.
Summary of the invention
The technical problem to be solved be to provide a kind of have strengthen bacteriostasis Quinoa saponin and
Preparation method, which overcomes the drawbacks described above of prior art, and the fungistatic effect of Quinoa saponin can be made to be obviously enhanced.
The technical problem to be solved is achieved through the following technical solutions:
A kind of preparation method with the Quinoa saponin strengthening bacteriostasis, comprises the following steps: pulverize Quinoa wheat bran
70% methanol extraction, filtering and concentrating extracting solution, ethyl acetate and n-butyl alcohol remove impurity, aqueous slkali modification, desalination, macroporous resin are pure
Changing, last lyophilization obtains Quinoa saponin.
Preferably, in technique scheme, comprise the following steps:
1) extract: Quinoa wheat bran is pulverized, cross 60 mesh sieves, add the methanol of 8-12 times amount 70%, in 45-65 DEG C of water-bath
Stirring is extracted, and obtains extracting solution；
2) concentrate: by extracting solution centrifugal filtration, then by filtrate concentrated by rotary evaporation to certain volume, obtain concentrated solution；
3) remove impurity: add the ethyl acetate of 2 times amount volumes, concussion layering in concentrated solution, remove ethyl acetate layer, then add
Enter the n-butyl alcohol of 2 times amount volumes, concussion layering, take n-butanol layer, volatilize in 50 DEG C of water-baths, obtain the thick saponin of Quinoa；
4) modified: with the Quinoa thick saponin solution of distilled water preparation 5-10g/L, to add 0.1-0.2 times amount volume 1mol/L
NaOH solution, at 90-95 DEG C, stirring reaction 2-4 hour, is cooled to room temperature, adds the salt acid for adjusting pH of 0.1mol/L to 7,
Obtain neutralizer；
5) desalination: by step 4) neutralizer that obtains is placed in bag filter, dialysis desalting, concentrate；
6) purification: by step 5) concentrated solution after desalination crosses AB-8 macroporous resin column, and carry out isolated and purified, collect purpose and wash
De-liquid, lyophilization obtains Quinoa saponin.
Preferably, in technique scheme, the condition of described 70% methanol extraction is: carry 45-65 DEG C of stirred in water bath
Take twice, each 3-5 hour, obtain extracting solution.
Preferably, in technique scheme, the condition that described filtration rotation is steamed is: by extracting solution centrifugal filtration, merge twice
Filtrate, concentrated by rotary evaporation under the conditions of temperature 50 C, vacuum 0.1Mpa.
Preferably, in technique scheme, described desalination processes is particularly as follows: be placed in the neutralizer after modification and retain
Molecular weight is in 100 daltonian bag filters, distilled water dialysis to without NaCl detect, in 55 DEG C, 0.1Mpa vacuum will dialyse
Trapped fluid is concentrated to certain volume.
Preferably, in technique scheme, described macroporous resin column isolation and purification method particularly as follows: respectively with water, 30%
With the ethanol elution of 80%, elution volume is 1 times of column volume, collects the part of 80% ethanol elution, volatilizes second in 50 DEG C of water-baths
Alcohol, obtains the thick saponin of Quinoa.
A kind of prepare according to any of the above-described described preparation method with the Quinoa saponin strengthening bacteriostasis
Quinoa saponin.
A kind of Quinoa prepared according to arbitrary described preparation method with the Quinoa saponin strengthening bacteriostasis
Saponin has the application in anti-halitosis product in preparation.
The present invention based on the principle that
The structure changing Quinoa saponin is processed, so that its fungistatic effect is obviously enhanced by alkali.
Technique scheme of the present invention, has the advantages that
The present invention can prepare the Quinoa saponin with enhancing fungistatic effect quickly and efficiently.Antibacterial culturing it is demonstrated experimentally that
The saponin fungistatic effect that the more non-alkali of Quinoa saponin after alkali process processes is obviously enhanced.Particularly through three kinds of oral cavity pathogen (teeth
Gum Detection of Porphyromonas, perfringens Fusobacterium, Fusobacterium nucleatum) In vitro culture it is demonstrated experimentally that alkali process after Quinoa saponin relatively
Non-alkali processes the bacteriostasis of Quinoa saponin and is remarkably reinforced.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail, in order to be further appreciated by the present invention.
In following example, the experimental technique of all uses if no special instructions, is conventional method.
Material used in following example, reagent etc., if no special instructions, all can be by being either commercially available.
Embodiment 1
A kind of have the Quinoa saponin strengthening bacteriostasis, and its preparation method is as follows:
(1) Quinoa wheat bran is pulverized, and crosses 60 mesh sieves, takes 200g and be placed in conical flask, adds the methanol 1600mL of 70%, 45
DEG C stirred in water bath extracts twice, each 5 hours；
(2) merging twice filtrate, temperature 50 C, vacuum 0.1Mpa, concentrated extracting solution to 80mL are steamed in rotation；
(3) concentrated solution adds the ethyl acetate of 160mL, concussion layering, removes ethyl acetate layer, adds the positive fourth of 160mL
Alcohol, concussion layering, take n-butanol layer and volatilize in 50 DEG C of water-baths, obtain the thick saponin of Quinoa；
(4) with the Quinoa thick saponin solution 1L of distilled water preparation 5g/L, NaOH solution 100mL of 1mol/L is added, in 90
At DEG C, stirring reaction 4 hours, are cooled to room temperature, add the salt acid for adjusting pH of 0.1mol/L to 7；
(5) being placed in by the neutralizer that (4) obtain in the bag filter that molecular cut off is 100D, distilled water dialysis is to without NaCl
Detection, in 55 DEG C, 0.1Mpa vacuum dialysis trapped fluid is concentrated to 100mL；
(6) use AB-8 macroporous resin concentrated solution that (5) are obtained to carry out isolated and purified, respectively with water, 30% and 80%
Ethanol elution, elution volume is 1 column volume；
(7) collecting the part of 80% ethanol elution, volatilize ethanol in 50 DEG C of water-baths, lyophilization prepares Quinoa saponin.
Embodiment 2
A kind of have the Quinoa saponin strengthening bacteriostasis, and its preparation method is as follows:
(1) Quinoa wheat bran is pulverized, and crosses 60 mesh sieves, takes 400g and be placed in conical flask, adds the methanol 3200mL of 70%, 65
DEG C stirred in water bath extracts twice, each 3 hours；
(2) merging twice filtrate, temperature 50 C, vacuum 0.1Mpa, concentrated extracting solution to 150mL are steamed in rotation；
(3) concentrated solution adds the ethyl acetate of 300mL, concussion layering, removes ethyl acetate layer, adds the positive fourth of 300mL
Alcohol, concussion layering, take n-butanol layer and volatilize in 50 DEG C of water-baths, obtain the thick saponin of Quinoa；
(4) with the Quinoa thick saponin solution 1L of distilled water preparation 10g/L, NaOH solution 200mL of 1mol/L is added, in 95
At DEG C, stirring reaction 2 hours, are cooled to room temperature, add the salt acid for adjusting pH of 0.1mol/L to 7；
(5) being placed in by the neutralizer that (4) obtain in the bag filter that molecular cut off is 100D, distilled water dialysis is to without NaCl
Detection, in 55 DEG C, 0.1Mpa vacuum dialysis trapped fluid is concentrated to 100mL；
(6) use AB-8 macroporous resin concentrated solution that (5) are obtained to carry out isolated and purified, respectively with water, 30% and 80%
Ethanol elution, elution volume is 1 column volume；
(7) collecting the part of 80% ethanol elution, volatilize ethanol in 50 DEG C of water-baths, lyophilization prepares Quinoa saponin.
Embodiment 3
A kind of have the Quinoa saponin strengthening bacteriostasis, and its preparation method is as follows:
(1) Quinoa wheat bran is pulverized, and crosses 60 mesh sieves, takes 800g and be placed in conical flask, adds the methanol 8000mL of 70%, 60
DEG C stirred in water bath extracts twice, each 4 hours；
(2) merging twice filtrate, temperature 50 C, vacuum 0.1Mpa, concentrated extracting solution to 200mL are steamed in rotation；
(3) concentrated solution adds the ethyl acetate of 400mL, concussion layering, removes ethyl acetate layer, adds the positive fourth of 400mL
Alcohol, concussion layering, take n-butanol layer and volatilize in 50 DEG C of water-baths, obtain the thick saponin of Quinoa；
(4) with the Quinoa thick saponin solution 1L of distilled water preparation 8g/L, NaOH solution 150mL of 1mol/L is added, in 92
At DEG C, stirring reaction 3 hours, are cooled to room temperature, add the salt acid for adjusting pH of 0.1mol/L to 7；
(5) being placed in by the neutralizer that (4) obtain in the bag filter that molecular cut off is 100D, distilled water dialysis is to without NaCl
Detection, in 55 DEG C, 0.1Mpa vacuum dialysis trapped fluid is concentrated to 150mL；
(6) use AB-8 macroporous resin concentrated solution that (5) are obtained to carry out isolated and purified, respectively with water, 30% and 80%
Ethanol elution, elution volume is 1 column volume；
(7) collecting the part of 80% ethanol elution, volatilize ethanol in 50 DEG C of water-baths, lyophilization prepares Quinoa saponin.
Embodiment 4
Embodiment 1,2,3 tool strengthens the Quinoa saponin of bacteriostasis, illustrates the present invention by antibacterial experiment in vitro
Antibacterial reinforced effects:
Method: use porphyromonas gingivalis (ATCC52066), perfringens Fusobacterium (ATCC28188), tool core shuttle bar
Bacterium (ATCC9533), for representing bacterium, checks the bacteriostasis of Quinoa saponin.Preparation Quinoa saponin series concentration solution, joins thin
Bacteria concentration is in 3 kinds of bacterium solution of 1 × 107cfu/mL, hatches 24h in 37 DEG C, measures minimum inhibitory concentration (MIC) and minimum bactericidal
Concentration (MBC).This experiment contrast group sample (non-alkali process Quinoa saponin) according to embodiment 3 do not include (4th) and (5th) step with
Prepared by outer step.
Table 1 is Quinoa saponin bacteriostatic activity test result.
Result proves (as shown in table 1), and alkali processes Quinoa saponin and is respectively less than MIC and MBC of three kinds of tested bacterium at non-alkali
Reason Quinoa saponin, show alkali process Quinoa saponin to porphyromonas gingivalis, perfringens Fusobacterium, Fusobacterium nucleatum antibacterial
Effect is better than non-alkali and processes Quinoa saponin.In three kinds of tested bacterium, alkali processes the Quinoa saponin inhibitory action to Fusobacterium nucleatum
The strongest.
Therefore, the Quinoa saponin that method produced according to the present invention prepares, it can be further as effective active composition
Being processed into and have anti-halitosis product, the dosage form of this product can select spray, decoction, powder, pill etc. various as required
Different dosage forms.
Although the present invention is open as above with embodiment, so it is not intended to limit the present invention, any people in the art
Member, without departing from the spirit and scope of the present invention, all can make various different selection and amendment, therefore the protection model of the present invention
Enclose and limited by claims and equivalents thereof.
Claims (8)
1. there is a preparation method for the Quinoa saponin strengthening bacteriostasis, comprise the following steps: Quinoa wheat bran is pulverized
70% methanol extraction, filtering and concentrating extracting solution, ethyl acetate and n-butyl alcohol remove impurity, aqueous slkali modification, desalination, macroporous resin are pure
Changing, last lyophilization obtains Quinoa saponin.
The preparation method with the Quinoa saponin strengthening bacteriostasis the most according to claim 1, it is characterised in that include
Following steps:
1) extract: Quinoa wheat bran is pulverized, cross 60 mesh sieves, add the methanol of 8-12 times amount 70%, 45-65 DEG C of stirred in water bath
Extract, obtain extracting solution；
2) concentrate: by extracting solution centrifugal filtration, then by filtrate concentrated by rotary evaporation to certain volume, obtain concentrated solution；
3) remove impurity: add the ethyl acetate of 2 times amount volumes, concussion layering in concentrated solution, remove ethyl acetate layer, add 2
The n-butyl alcohol of times amount volume, concussion layering, take n-butanol layer, volatilize in 50 DEG C of water-baths, obtain the thick saponin of Quinoa；
4) modified: with the Quinoa thick saponin solution of distilled water preparation 5-10g/L, to add 0.1-0.2 times amount volume 1mol/L's
NaOH solution, stirring reaction 2-4 hour at 90-95 DEG C, it is cooled to room temperature, the salt acid for adjusting pH of addition 0.1mol/L, to 7, obtains
Neutralizer；
5) desalination: by step 4) neutralizer that obtains is placed in bag filter, dialysis desalting, concentrate；
6) purification: by step 5) concentrated solution after desalination crosses AB-8 macroporous resin column, and carry out isolated and purified, collect purpose eluting
Liquid, lyophilization obtains Quinoa saponin.
The preparation method with the Quinoa saponin strengthening bacteriostasis the most according to claim 2, it is characterised in that described
The condition of 70% methanol extraction is: extracts twice 45-65 DEG C of stirred in water bath, each 3-5 hour, obtains extracting solution.
The preparation method with the Quinoa saponin strengthening bacteriostasis the most according to claim 2, it is characterised in that described
The condition filtering rotation steaming is: by extracting solution centrifugal filtration, merge twice filtrate, under the conditions of temperature 50 C, vacuum 0.1Mpa
Concentrated by rotary evaporation.
The preparation method with the Quinoa saponin strengthening bacteriostasis the most according to claim 2, it is characterised in that described
Desalination processes particularly as follows: it is in 100 daltonian bag filters that the neutralizer after modification is placed in molecular cut off, distilled water
Dialysis to without NaCl detection, in 55 DEG C, 0.1Mpa vacuum dialysis trapped fluid is concentrated to certain volume.
The preparation method with the Quinoa saponin strengthening bacteriostasis the most according to claim 2, it is characterised in that described
Macroporous resin column isolation and purification method is particularly as follows: use water, 30% and the ethanol elution of 80% respectively, and elution volume is 1 times of cylinder
Long-pending, collect the part of 80% ethanol elution, volatilize ethanol in 50 DEG C of water-baths, obtain the thick saponin of Quinoa.
7. one kind is prepared into according to the arbitrary described preparation method with the Quinoa saponin strengthening bacteriostasis of claim 1-6
The Quinoa saponin arrived.
8. prepare according to the arbitrary described preparation method with the Quinoa saponin strengthening bacteriostasis of claim 1-6
Quinoa saponin has the application in anti-halitosis product in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610529182.0A CN106176847B (en) | 2016-07-06 | 2016-07-06 | A kind of quinoa saponin(e and preparation method thereof with enhancing bacteriostasis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610529182.0A CN106176847B (en) | 2016-07-06 | 2016-07-06 | A kind of quinoa saponin(e and preparation method thereof with enhancing bacteriostasis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106176847A true CN106176847A (en) | 2016-12-07 |
| CN106176847B CN106176847B (en) | 2019-11-08 |
Family
ID=57465739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610529182.0A Active CN106176847B (en) | 2016-07-06 | 2016-07-06 | A kind of quinoa saponin(e and preparation method thereof with enhancing bacteriostasis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106176847B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107519068A (en) * | 2017-03-13 | 2017-12-29 | 王高兴 | A kind of preparation method for the sun-proof Moisturizer of fermentation quinoa trehalose that saponin(e is extracted using quinoa chaff shell |
| CN107913296A (en) * | 2017-12-14 | 2018-04-17 | 云南德彩堂生物医药科技有限公司 | A kind of attached raw material of the high physiological activity quinoa of high stable and preparation method and application |
| CN109223809A (en) * | 2018-10-22 | 2019-01-18 | 薛鹏 | A kind of quinoa astragalin composition and its application in terms of antimicrobial agent |
| CN112675066A (en) * | 2021-01-20 | 2021-04-20 | 忻州师范学院 | Quinoa saponin hand sanitizer and preparation method thereof |
| CN113876824A (en) * | 2021-11-09 | 2022-01-04 | 河北农业大学 | Quinoa saponin and wild chrysanthemum flower composition, preparation method and application thereof |
| CN115028673A (en) * | 2022-06-13 | 2022-09-09 | 安徽省农业科学院棉花研究所 | Extraction and purification method of quinoa saponin |
| CN115501261A (en) * | 2022-10-14 | 2022-12-23 | 山西大学 | Quinoa bran water-soluble terpene extract as well as preparation method and application thereof |
-
2016
- 2016-07-06 CN CN201610529182.0A patent/CN106176847B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| MACARENA STUARDO等: ""Antifungal properties of quinoa (Chenopodium quinoa Willd) alkali treated saponins against Botrytis cinerea"", 《IND CROP PROD.》 * |
| TIWATT KULJANABHAGAVAD等: ""Biological activities and chemistry of saponins from Chenopodium quinoa Willd."", 《PHYTOCHEM REV》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107519068A (en) * | 2017-03-13 | 2017-12-29 | 王高兴 | A kind of preparation method for the sun-proof Moisturizer of fermentation quinoa trehalose that saponin(e is extracted using quinoa chaff shell |
| CN107913296A (en) * | 2017-12-14 | 2018-04-17 | 云南德彩堂生物医药科技有限公司 | A kind of attached raw material of the high physiological activity quinoa of high stable and preparation method and application |
| CN109223809A (en) * | 2018-10-22 | 2019-01-18 | 薛鹏 | A kind of quinoa astragalin composition and its application in terms of antimicrobial agent |
| CN112675066A (en) * | 2021-01-20 | 2021-04-20 | 忻州师范学院 | Quinoa saponin hand sanitizer and preparation method thereof |
| CN113876824A (en) * | 2021-11-09 | 2022-01-04 | 河北农业大学 | Quinoa saponin and wild chrysanthemum flower composition, preparation method and application thereof |
| CN115028673A (en) * | 2022-06-13 | 2022-09-09 | 安徽省农业科学院棉花研究所 | Extraction and purification method of quinoa saponin |
| CN115028673B (en) * | 2022-06-13 | 2023-09-05 | 安徽省农业科学院棉花研究所 | Extraction and purification method of quinoa saponins |
| CN115501261A (en) * | 2022-10-14 | 2022-12-23 | 山西大学 | Quinoa bran water-soluble terpene extract as well as preparation method and application thereof |
| CN115501261B (en) * | 2022-10-14 | 2023-09-22 | 山西大学 | Quinoa bran water-soluble terpenoid extract and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106176847B (en) | 2019-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106176847A (en) | A kind of have Quinoa saponin strengthening bacteriostasis and preparation method thereof | |
| JP5269160B2 (en) | Bioactive liquid composition and method for producing the same | |
| KR102600268B1 (en) | Industrial use method of Stevia rebaudiana and its stevioside and chlorogenic acid | |
| CN101565411A (en) | Method for extracting fucoxanthin from brown algae | |
| CN105267275B (en) | Method for extracting flavone from chrysanthemum | |
| CN101973853A (en) | Method for extracting hydroxytyrosol | |
| CN102304501A (en) | Complex enzyme preparation and application and method thereof for extracting plant polysaccharides by using same | |
| KR101676292B1 (en) | Cosmetic composition comprising an mixed extract of tangle, okra and yam and manufacturing method thereof | |
| CN105367424A (en) | Method for preparing high-purity chlorogenic acid from eupatorium adenophorum Spreng | |
| CN102424678A (en) | High-purity mangiferin prepared from leaves and twigs of aquilaria sinensis and preparation method thereof | |
| CN102218087B (en) | Method for preparing total flavone extracts of pagoda flower | |
| CN102132707B (en) | Extraction method capable of improving antibacterial activity of extract of leaves of fagaceae plant | |
| KR20070103213A (en) | Isolation process for proanthocyanidin from the bark of pine tree | |
| CN105192820A (en) | Blood-fat-reducing cordyceps militaris polysaccharide beverage and preparing method thereof | |
| CN101810317B (en) | Preparation method of canophyllic polyphenol and application thereof | |
| CN110680847A (en) | Method for extracting and purifying cannabinoids | |
| CN107556348A (en) | Acryloyl acid esters compound and preparation method thereof | |
| CN1202058C (en) | New process of preparing tea polyphenol with high catechin content and low caffine content | |
| CN101875685A (en) | Lysimachia foenum-graecum hance saponin extract having more than or equal to 30 percent of saponin and extraction process thereof | |
| CN104606264A (en) | Extraction method of sterculia nobilis polyphenol | |
| CN104523851A (en) | Method for extracting polygonum orientale pericarp general flavone extract product | |
| CN106389180A (en) | Ginkgo proanthocyanidin-polysaccharide mixing extract, preparation method and applications thereof | |
| CN106316797A (en) | Method for preparing hydroxytyrosol by using immobilized enzymic method coupled film technique | |
| CN104432114A (en) | Method for extracting dietary fibres from passion fruit seeds | |
| Ha et al. | Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |