CN105866314A - Detection method for analyzing content of macamides in Maca by virtue of HPLC - Google Patents
Detection method for analyzing content of macamides in Maca by virtue of HPLC Download PDFInfo
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- CN105866314A CN105866314A CN201610373483.9A CN201610373483A CN105866314A CN 105866314 A CN105866314 A CN 105866314A CN 201610373483 A CN201610373483 A CN 201610373483A CN 105866314 A CN105866314 A CN 105866314A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
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Abstract
The invention discloses a detection method for analyzing the contents of macamides in Maca by virtue of HPLC. According to the detection method, the contents of nine macamides in the Maca are quantitatively determined by taking macamides as a detection target. The macamides can act on ''hormone balance secretion system'' of hypothalamus-hypophysis-gland of a human body and promote the dynamic balance of hormones secreted by a human body, so that the internal environment of the human body is improved, and the human body tends to be in a healthy state. Therefore, the determination of the contents of macamides in the Maca is very important. By detecting through an HPLC method, the method is simple, convenient and feasible, and the result is reliable. The method can be taken as a novel detection method for the contents of macamides in the Maca for popularization and application and can be taken as basis for identifying raw materials and product quality of the Maca.
Description
Technical field
The present invention relates to a kind of use HPLC to analyze the detection method of macamide content in Lepidinm meyenii Walp, belong to
In natural plant active field of molecular detection.
Background technology
Lepidinm meyenii Walp, makees again Maca (Maca, Lepidium meyemii Walp), belongs to Cruciferae (Cruciferae)
Separate row Vegetable spp likeness in form Radix Raphani tuber class is annual or 2 years raw herbaceous plant, originate in the middle part of Peru extra large
Pull out the mountain area, Andean, South America of 3500～4500 meters, belong to pure natural plant.Continuous along with scientific research
Deeply, researcher finds that Lepidinm meyenii Walp not only has the effect improving fertility, also has resisting fatigue, delays
Solve climacteric syndrome shape, anti-prostatic hyperplasia, antioxidation, slow down aging and the important effect such as anticancer.
2011 we to ratify pueraria root powder be new resource food.Containing macamide, Lepidinm meyenii Walp alkene, mustard in Lepidinm meyenii Walp
The health-care effect active component such as seed oil glycosides, are a kind of pure naturals that can provide full nutrition material to human body
Food resource.Fresh Lepidinm meyenii Walp contains protein, fat, carbohydrate, cellulose and human body very
Abundant aminoacid etc., Lepidinm meyenii Walp has resisting fatigue, defying age, enhancing immunity, endocrine regulation system
System, improve sexual function, improve fertility, improve sleep, the effect such as improve the quality of living, in recent years
Come, the fashionable world of product such as food with Lepidinm meyenii Walp as raw material production, health promoting wine, Maca capsule, become
One of whole world situation of selling well health product.
Owing to Lepidinm meyenii Walp is expensive, some lawless persons utilize other inexpensive material similar to Lepidinm meyenii Walp
Pretend to be Lepidinm meyenii Walp.Lawless person is more had to add a certain proportion of Semen Maydis powder or mealy potato in pueraria root powder to seize
Take unlawful interests.The appearance of these adulterants has greatly injured the rights and interests of consumer.Although there being patent report
Road uses ITS sequence can differentiate Lepidinm meyenii Walp, but cannot know the macamide isoreactivity molecule in Lepidinm meyenii Walp
How many kinds, content is had to have the problems such as how many.(Chinese patent " a kind of discriminating certified products Lepidinm meyenii Walp, adulterant agate
Coffee and the doping ITS sequence of Lepidinm meyenii Walp and method " CN 102952878B).
U.S. foreign citizen of Chinese origin botanist Zheng B.L finds distinctive two kinds of unique activity in Lepidinm meyenii Walp
Material macamide and Lepidinm meyenii Walp alkene, wherein macamide main component has: N-benzyl palmitoyl amine, N-
Benzyl-9Z-octadecenamide, N-benzyl-(9Z, 12Z)-ten eight carbon diene amide, N-benzyl-
(9Z, 12Z, 15Z)-N-Isobutyl-2E,4E,12Z-octadecatrienamide, N-benzyl kemanide S, and N-benzyl decoyl
Amine, N-benzyl-16-hydroxyl-9-aoxidize-10E, 12E, 14E-pungent certain herbaceous plants with big flowers three acrylamide, N-benzyl-16-hydroxyl
Base-9,17 homologues such as 16-dioxy-10E, 12E, 14E-pungent certain herbaceous plants with big flowers three acrylamide, macamide exists
In Maca extract, content is about 0.2-0.4%.With HPLC, macamide is carried out quantitative analysis,
By the kind of macamide bioactive molecule in LC-MS fingerprint detail drawing Analysis and Identification Lepidinm meyenii Walp.At present this
Bright can measure nine kinds of macamide content, with verification result as foundation, calculate and recommend Lepidinm meyenii Walp product to make
With dosage, the science of reaching takes the purpose of Lepidinm meyenii Walp.
Summary of the invention
Purpose: for solving the deficiencies in the prior art, irregular not for Lepidinm meyenii Walp product quality in the market
Together, it is impossible to measure content and the kind of macamide of macamide bioactive molecule in Lepidinm meyenii Walp, it is impossible to mirror
The technological deficiencies such as the quality of other Lepidinm meyenii Walp product, the present invention provides a kind of HPLC of employing to analyze agate in Lepidinm meyenii Walp
The detection method of coffee amide content.
Technical scheme: for solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of HPLC is used to analyze the detection method of macamide content, the color of HPLC in Lepidinm meyenii Walp
Analysis of spectrum condition is as follows:
1) chromatographic column uses anti-phase C18 chromatographic column；
2) flowing phase composition is: acetonitrile-water-acid, and in flowing mutually, the volume ratio of each composition is: acetonitrile and
Water ratio is 4～10:1, the 0.05-1% that ratio is aqueous phase of acid.
3) acid includes but not limited to formic acid, glacial acetic acid, trifluoroacetic acid, trichloroacetic acid, phosphoric acid, sulphuric acid.
Acid is preferably formic acid, glacial acetic acid, trifluoroacetic acid.
4) ultraviolet detection wavelength is 210-254nm；Optimal wavelength is 210nm；
5) chromatogram column temperature is: 15～35 DEG C；Preferred column temperature is 20～25 DEG C.
By central composite design method, optimize chromatographiccondition, colors can be significantly improved
Spectral peak separating effect, shortens analysis time, chromatography collection of illustrative plates favorable reproducibility；Draw optimal chromatostrip
Part.
6) macamide reference substance
Select main nine kinds of macamide in Lepidinm meyenii Walp to detect, for 1-9 macamide, be respectively (1)
Meta-methoxy benzyl linolenamide 1, (2) benzyl linolenamide 2, (3) meta-methoxy benzyl Asia oleoyl
Amine 3, (4) benzyl Asia oleamide 4, (5) meta-methoxy benzyl hexadecane oleamide 5, (6) benzyl
Base palmitic amide 6, (7) benzyl spunyarn amide 7, (8) benzyl kemanide S 8, (9) benzyl
Base heptadecane amide 9；
Its chemical structural formula and title are as follows:
Macamide chemical structural formula
Note: above-mentioned nine kinds of macamide reference substances are from Hui Bo bio tech ltd, Jiangsu, sample
Purity is more than 95%.This reference substance Stability Analysis of Structures, can preserve at-20 DEG C throughout the year, be configured to standard molten
After liquid ,-20 DEG C of airtight preservations also can preserve 3-6 month.
7) drafting of standard curve
Take macamide reference substance (1), (3), (6), (7), (8), (9) each 4mg, Lepidinm meyenii Walp acyl
Amine reference substance (2), (4) and (5) each 10mg, uses acetonitrile to dissolve and constant volume is in 100mL capacity
In Ping, it is that nine kinds of macamide mix target standard solution.Take respectively standard solution 0.1,0.2,0.4,
0.6,0.8 and 1.0mL, add 0.9,0.8,0.6,0.4,0.2 and 0mL acetonitrile to be configured to standard series each
Concentration detects for HPLC；Sample size is 10 μ l；
8) need testing solution
Lepidinm meyenii Walp sample, through smashing, crosses 60 mesh sieves (particle diameter≤0.21mm), accurately weighs 1g pueraria root powder (accurate
Accurate to 1.0000g ± 0.0010g) in 100-150ml round-bottomed flask, add after adding 40-60ml petroleum ether
Enter condensing tube, be placed in supersonic cleaning machine, 40-50 DEG C, supersound extraction 30-90min；Ultrasonic complete after
Shaking up, extracting solution and Lepidinm meyenii Walp slag are together poured in 50-100ml centrifuge tube, 5000rpm is centrifuged 10
-20min；Being poured into by supernatant in 100ml round-bottomed flask, rotation adds 4-6ml acetonitrile after being evaporated, fully
Draw to 5ml volumetric flask or 10ml volumetric flask after shaking up dissolving extract, then add 0.8-1.5ml acetonitrile extremely
Round-bottomed flask is drawn to above-mentioned volumetric flask after shaking up, and takes appropriate acetonitrile and is settled to 5ml or 10ml, warp
After 0.22 μm filtering with microporous membrane, detect for HPLC；
9) result calculates
In sample, the content of nine kinds of macamide is calculated by formula (1) respectively:
Macamide total content is calculated by formula (2):
xAlways=∑ xi..........................................................(2)
In formula:
xiThe content of macamide in sample, unit is milligrams per kilogram (mg/kg)；
mI marksThe quality of macamide reference substance, unit is milligram (mg)；
PI marksThe content of macamide reference substance；
miSample mass, unit is gram (g)；
AI marksThe peak area meansigma methods of macamide reference substance；
AiThe peak area meansigma methods of sample macamide sample；
xAlwaysIn sample, macamide content is 9 kinds of amide content summations.
Preferably, described acid is formic acid, glacial acetic acid, trifluoroacetic acid, trichloroacetic acid, phosphorus
One or more in acid, sulphuric acid.It is further preferred that acid is in formic acid, glacial acetic acid, trifluoroacetic acid
One or more.
Preferably, ultraviolet detection wavelength is 210nm.
Preferably, chromatogram column temperature is 20～25 DEG C.
Beneficial effect: a kind of of present invention offer uses HPLC to analyze macamide content in Lepidinm meyenii Walp
Detection method, carries out quantitative analysis with HPLC to macamide, is analyzed by LC-MS fingerprint detail drawing
Identify the kind of macamide bioactive molecule in Lepidinm meyenii Walp.The chromatographiccondition obtained with optimization of the present invention,
Under conditions of isocratic elution, in 30min, just can concurrently separate the chromatograph analyzing more than 10
Peak, confirms 1-9 peak therein by compound control product, be respectively (1), (2), (3),
(4)、(5)、(6)、(7)、(8)、(9)；The current present invention can measure nine kinds of macamide and contain
Amount, with verification result as foundation, calculates and recommends Lepidinm meyenii Walp product using dosage, and the science of reaching takes Lepidinm meyenii Walp
Purpose.Compared with method in the past, not only solve asking of the more difficult separation of macamide in Lepidinm meyenii Walp
Topic, and solve the quantitative determination of macamide in Lepidinm meyenii Walp, because have employed Gradient elution
Method, repeatability is good.
Accompanying drawing explanation
Fig. 1 is the HPLC collection of illustrative plates of Lepidinm meyenii Walp sample；
Fig. 2 is the HPLC collection of illustrative plates of macamide standard substance；
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.Following each embodiment is only used for
The present invention is described, the present invention is not limited.
Embodiment 1
The method of macamide content in employing HPLC analysis Lepidinm meyenii Walp:
1. instrument and material
Agilent 1260 high performance liquid chromatograph (Agilent company of the U.S.), UV detector, chromatographic column
ODS (4.6 × 250mm, 5 μm) (Agilent company of the U.S.).Chromatograph acetonitrile, chromatograph formic acid, double steamings
Water (laboratory self-control).
2. chromatographic condition
Chromatographic column: ODS (4.6 × 250mm, 5 μm) (Agilent company of the U.S.)；
Flowing phase: isocratic elution, acetonitrile: water is 90:10, wherein aqueous phase contains the formic acid of 0.2%；Flow velocity is 1
mL·min-1；Detection wavelength is 210nm；Sampling volume is 10 μ L.Column temperature 25 DEG C.
The drafting of standard curve
3. the preparation of control sample
Take macamide reference substance (1), (3), (6), (7), (8), (9) each 4mg, Lepidinm meyenii Walp acyl
Amine reference substance (2), (4) and (5) each 10mg, uses acetonitrile to dissolve and constant volume holds in 100mL
In measuring bottle, being that nine kinds of macamide mix target standard solution, sample size is 10 μ l.Examine for HPLC
Survey.
4. need testing solution
Lepidinm meyenii Walp sample, through smashing, crosses 60 mesh sieves (particle diameter≤0.21mm), accurately weighs 1g pueraria root powder (accurate
Accurate to 1.0000g ± 0.0010g) in 100ml round-bottomed flask, add cold after adding 40ml petroleum ether
Solidifying pipe, is placed in supersonic cleaning machine, 40-50 DEG C, supersound extraction 90min.Ultrasonic complete after shake up,
Extracting solution and Lepidinm meyenii Walp slag together being poured in 50ml centrifuge tube, 5000rpm is centrifuged 20min.By supernatant
Liquid is poured in 100ml round-bottomed flask, and rotation adds 4ml acetonitrile after being evaporated, and fully shakes up dissolving extract
Rear absorption is to 5ml volumetric flask, then adds absorption extremely above-mentioned capacity after 0.8ml acetonitrile shakes up to round-bottomed flask
Bottle, takes appropriate acetonitrile and is settled to 5ml, after 0.22 μm filtering with microporous membrane, examines for HPLC
Survey.
5. detecting according to control sample, test sample, test sample, control sample, the peak area between sample is relative
Error is at 98%-102%.Average.
6. result calculates
In sample, the content of nine kinds of macamide is all calculated by formula (1), and macamide total content is pressed
Formula (2) calculates.
Embodiment 2
1. instrument and material
Agilent 1260 high performance liquid chromatograph (Agilent company of the U.S.), UV detector, chromatographic column
ODS (4.6 × 250mm, 5 μm) (Shimadzu ODS-3C18).Chromatograph acetonitrile, chromatograph trifluoroacetic acid,
Distilled water (laboratory self-control).
2. chromatographic condition
Chromatographic column: ODS (4.6 × 250mm, 5 μm) (Shimadzu ODS-3C18)；
Flowing phase: isocratic elution, acetonitrile: water is 85:15, wherein aqueous phase contains the trifluoroacetic acid of 0.05%；Flow velocity
For 0.8mL min-1；Detection wavelength is 210nm；Sampling volume is 10 μ L.Column temperature 30 DEG C.
The drafting of standard curve
3. the preparation of control sample
Take macamide reference substance (1), (3), (6), (7), (8), (9) each 4mg, Lepidinm meyenii Walp acyl
Amine reference substance (2), (4) and (5) each 10mg, uses acetonitrile to dissolve and constant volume holds in 100mL
In measuring bottle, being that nine kinds of macamide mix target standard solution, sample size is 10 μ l.Examine for HPLC
Survey.Macamide standard substance HPLC spectrogram is as shown in Figure 2.
4. need testing solution
Lepidinm meyenii Walp sample, through smashing, crosses 60 mesh sieves (particle diameter≤0.21mm), accurately weighs 1g pueraria root powder (accurate
Accurate to 1.0000g ± 0.0010g) in 150ml round-bottomed flask, add cold after adding 60ml petroleum ether
Solidifying pipe, is placed in supersonic cleaning machine, 40-50 DEG C, supersound extraction 300min.Ultrasonic complete after shake up,
Extracting solution and Lepidinm meyenii Walp slag together being poured in 100ml centrifuge tube, 5000rpm is centrifuged 10min.By upper
Clear liquid is poured in 100ml round-bottomed flask, and rotation adds 6ml acetonitrile after being evaporated, and fully shakes up dissolving and extracts
Draw after thing to 5ml volumetric flask, then add absorption extremely above-mentioned appearance after 1.5ml acetonitrile shakes up to round-bottomed flask
Measuring bottle, takes appropriate acetonitrile and is settled to 10ml, after 0.22 μm filtering with microporous membrane, examines for HPLC
Survey；Lepidinm meyenii Walp sample HPLC schemes as shown in Figure 1；
5. detecting according to control sample, test sample, test sample, control sample, the peak area between sample is relative
Error is at 98%-102%.Average.
6. result calculates
In sample, the content of nine kinds of macamide is all calculated by formula (1), and macamide total content is pressed
Formula (2) calculates.
The effect of the present invention: the chromatographiccondition obtained with optimization of the present invention, at isocratic elution
Under the conditions of, just can concurrently separate the chromatographic peak analyzing more than 10 in 30min, pass through chemical combination
Thing reference substance confirms 1-9 peak therein, be respectively (1), (2), (3), (4), (5),
(6)、(7)、(8)、(9)；Compared with method, not only solve Lepidinm meyenii Walp acyl in Lepidinm meyenii Walp in the past
The problem of the more difficult separation of amine, and solve the quantitative determination of macamide in Lepidinm meyenii Walp, because using
The method of Gradient elution, repeatability is good.
Nine kinds of macamide ranges of linearity, regression equation, correlation coefficient and detection limit, precision is as shown in table 1:
1 nine kinds of macamide of table mixed mark range of linearity, regression equation, correlation coefficient, detection limit and precision
* Y:peak area；X:mass concentration, mg/L
Embodiment 3 precision, stability and replica test
Precision test: take same sample and prepare sample liquid by above-mentioned sample treatment, continuous sample introduction 6 times,
Each 10 μ l, detect the content of composition under identical chromatographic condition, calculate it and relatively mark in analyzing liquid sample
Quasi-deviation RSD value.Experimental result draws, the RSD value of nine kinds of each component contents of macamide in Lepidinm meyenii Walp
It is 0.016%～2.10%, is shown in Table 2, show that precision is good.
Embodiment 4 stability test: take the sample liquid just prepared, every 0,2,4,8,12,24h enters
Sample once, each 10 μ l, according to sample liquid detects the content of composition, calculate its RSD value.In Lepidinm meyenii Walp 9
The RSD value planting macamide content is 0.12%～2.80% (referring to table 2).Result shows, test sample
The chromatographic results that solution measures in 24h is stable.
Embodiment 5 replica test: take same Lepidinm meyenii Walp sample, by 6 parts of sample liquid of the parallel preparation of sample treatment,
Sample introduction 10 μ l carries out chromatography, according to detection component content in the sample, calculates RSD value.Knot
Fruit draws, the RSD value of 9 kinds of macamide content is 1.12%～4.82% (referring to table 2), measures
Repeatability is good.
Table 2 precision, stability and replica test result (n=6)
Embodiment 6 response rate is tested
Claiming Lepidinm meyenii Walp sample 1.0000g, 5.0, under two mark-on levels of 10.0mg, Parallel testing 3 times, adds
The mark response rate the results are shown in Table 3, owing to macamide each content difference is relatively big, so response rate fluctuation is bigger.
The response rate of nine kinds of macamide compositions is all between 76～126%.
The response rate of macamide and relative standard deviation (n=3) in table 3 sample
Below disclosing the present invention with preferred embodiment, so it is not intended to limiting the invention, all adopts
The technical scheme obtained by equivalent or equivalent transformation mode, all falls within the protection model of the present invention
Within enclosing.
Claims (5)
1. use HPLC to analyze a detection method for macamide content in Lepidinm meyenii Walp, HPLC's
Chromatographiccondition is as follows:
1) chromatographic column uses anti-phase C18 chromatographic column；
2) flowing phase composition is: acetonitrile-water-acid, and in flowing mutually, the volume ratio of each composition is: acetonitrile and
Water ratio is 4～10:1, the 0.05-1% that ratio is aqueous phase of acid；
3) ultraviolet detection wavelength is 210-254nm；
4) chromatogram column temperature is: 15～35 DEG C；
5) macamide reference substance:
Select nine kinds of main macamide in Lepidinm meyenii Walp to detect, for 1-9 macamide, be respectively
(1) meta-methoxy benzyl linolenamide 1, (2) benzyl linolenamide 2, (3) meta-methoxy benzyl
Sub-oleamide 3, (4) benzyl Asia oleamide 4, (5) meta-methoxy benzyl hexadecane oleamide 5, (6)
Benzyl palmitic amide 6, (7) benzyl spunyarn amide 7, (8) benzyl kemanide S 8, (9)
Benzyl heptadecane amide 9；
Its chemical structural formula and title are as follows:
Macamide chemical structural formula
6) drafting of standard curve:
Take macamide reference substance (1), (3), (6), (7), (8), (9) each 4mg, Lepidinm meyenii Walp acyl
Amine reference substance (2), (4) and (5) each 10mg, uses acetonitrile to dissolve and constant volume is in 100mL capacity
In Ping, it is that nine kinds of macamide mix target standard solution；Take respectively standard solution 0.1,0.2,0.4,
0.6,0.8 and 1.0mL, add 0.9,0.8,0.6,0.4,0.2 and 0mL acetonitrile to be configured to standard series each
Concentration detects for HPLC；Sample size is 10 μ l；
7) need testing solution:
Lepidinm meyenii Walp sample, through smashing, crosses 60 mesh sieves (particle diameter≤0.21mm), accurately weighs 1g pueraria root powder (accurate
Accurate to 1.0000g ± 0.0010g) in 100-150ml round-bottomed flask, add after adding 40-60ml petroleum ether
Enter condensing tube, be placed in supersonic cleaning machine, 40-50 DEG C, supersound extraction 30-90min；Ultrasonic complete after
Shaking up, extracting solution and Lepidinm meyenii Walp slag are together poured in 50-100ml centrifuge tube, 5000rpm is centrifuged 10
-20min；Being poured into by supernatant in 100ml round-bottomed flask, rotation adds 4-6ml acetonitrile after being evaporated, fully
Draw to 5ml volumetric flask or 10ml volumetric flask after shaking up dissolving extract, then add 0.8-1.5ml acetonitrile extremely
Round-bottomed flask is drawn to above-mentioned volumetric flask after shaking up, and takes appropriate acetonitrile and is settled to 5ml or 10ml, warp
After 0.22 μm filtering with microporous membrane, detect for HPLC；
8) result calculates:
In sample, the content of nine kinds of macamide is calculated by formula (1) respectively:
Macamide total content is calculated by formula (2):
xAlways=∑ xi..........................................................(2)
In formula:
xiThe content of macamide in sample, unit is milligrams per kilogram (mg/kg)；
mI marksThe quality of macamide reference substance, unit is milligram (mg)；
PI marksThe content of macamide reference substance；
miSample mass, unit is gram (g)；
AI marksThe peak area meansigma methods of macamide reference substance；
AiThe peak area meansigma methods of sample macamide sample；
xAlwaysIn sample, macamide content is 9 kinds of amide content summations.
Employing HPLC the most according to claim 1 analyzes the inspection of macamide content in Lepidinm meyenii Walp
Survey method, it is characterised in that: described acid be formic acid, glacial acetic acid, trifluoroacetic acid, trichloroacetic acid,
One or more in phosphoric acid, sulphuric acid.
Employing HPLC the most according to claim 2 analyzes the inspection of macamide content in Lepidinm meyenii Walp
Survey method, it is characterised in that: acid is one or more in formic acid, glacial acetic acid, trifluoroacetic acid.
Employing HPLC the most according to claim 1 analyzes the inspection of macamide content in Lepidinm meyenii Walp
Survey method, it is characterised in that: ultraviolet detection wavelength is 210nm.
Employing HPLC the most according to claim 1 analyzes the inspection of macamide content in Lepidinm meyenii Walp
Survey method, it is characterised in that: chromatogram column temperature is 20～25 DEG C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106526033A (en) * | 2016-12-30 | 2017-03-22 | 大连大学 | Method for simultaneously testing 11 macamides content of maca |
| CN106596766A (en) * | 2016-12-13 | 2017-04-26 | 中山大学 | Analysis method for content of macamide B in maca supercritical CO2 extract |
| CN107748211A (en) * | 2017-09-14 | 2018-03-02 | 大连大学 | A kind of method using 5 kinds of macamides in deep co-melting solvent extraction measure maca |
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| CN106526033A (en) * | 2016-12-30 | 2017-03-22 | 大连大学 | Method for simultaneously testing 11 macamides content of maca |
| CN107748211A (en) * | 2017-09-14 | 2018-03-02 | 大连大学 | A kind of method using 5 kinds of macamides in deep co-melting solvent extraction measure maca |
| CN107748211B (en) * | 2017-09-14 | 2020-05-19 | 大连大学 | Method for extracting and measuring 5 macamides in maca by using deep eutectic solvent |
| CN110596264A (en) * | 2019-09-03 | 2019-12-20 | 谱尼测试集团股份有限公司 | Method for rapidly screening macamides compounds through UPLC-Q-TOF |
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