CN106526033A - Method for simultaneously testing 11 macamides content of maca - Google Patents
Method for simultaneously testing 11 macamides content of maca Download PDFInfo
- Publication number
- CN106526033A CN106526033A CN201611258172.4A CN201611258172A CN106526033A CN 106526033 A CN106526033 A CN 106526033A CN 201611258172 A CN201611258172 A CN 201611258172A CN 106526033 A CN106526033 A CN 106526033A
- Authority
- CN
- China
- Prior art keywords
- benzyl
- methoxy
- amine
- macamide
- benzyls
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Abstract
The invention relates to a high efficiency liquid chromatography for simultaneously testing the content of main macamides types of maca, and in particular to a method for simultaneously testing 11 macamides content of maca, and belongs to the technical field of analytical chemistry. The technical key points comprises the steps of (1) preparing to-be-tested solution, (2) preparing control article mixture solution, (3) establishing a standard curve, (4) testing the macamides content in the to-be-tested solution. The testing method applies the gradient elution method with better resolution, can simultaneously test 11 macamides content of maca. The method uses only acetonitrile and water as the mobile phase, thus solving the problem that trifluoroacetic acid causes huge loss of chromatographic column and leads to complex washing procedure and the like, thus making the operation convenient.
Description
Technical field
The invention belongs to technical field of analytical chemistry, and in particular to a kind of to determine 11 kinds of macamide contents in Lepidinm meyenii Walp simultaneously
Method.
Background technology
A kind of annual herb plant of the Lepidinm meyenii Walp (Lepidium meyenii) for Cruciferae separate row Vegetable spp, grows earliest
The andes region in the central authorities of Peru in height above sea level more than 4000 meters, with the edible history of 2000.The rhizome part ratio of Lepidinm meyenii Walp
Aerial partss have higher nutritive value, and root is similar to turnip radish, and profile color has purple, black, yellow etc..Research shows that Lepidinm meyenii Walp has
There are abundant nutritive value and medical value, Lepidinm meyenii Walp also has many traditional curative effects, such as cathartic, treatment rheumatism, exhale
Inhale tract disease, dysmenorrhea and female climacteric syndrome etc..In addition, Lepidinm meyenii Walp also have improve sexual function, improve people and animals fertility,
The function such as fatigue alleviating and antioxidation.Based on above feature, Lepidinm meyenii Walp is referred to as " Peru's Radix Ginseng ".Macamide in Lepidinm meyenii Walp is regarded
For main active functional component, it is the major criterion for evaluating Lepidinm meyenii Walp quality.
It is many to the detection method of macamide at present, high performance liquid chromatography (high performance liquid
Chromatography, HPLC) technology is applied to the content detection of macamide and also has been reported that, but it is same in the method having been reported
When determine macamide species it is less.
The content of the invention
The employing HPLC methods that the present invention is provided can determine the content of 11 kinds of macamides in Lepidinm meyenii Walp simultaneously, and using foundation
Method determines the content of 20 different samples, and testing result is accurately and reliably, simple and efficient, be a kind of analysis macamide class into
The effective way divided, can provide theoretical foundation for the exploitation of macamide in Lepidinm meyenii Walp, be Lepidinm meyenii Walp breed breeding and excellent product
Plant and promote based theoretical.
Technical scheme is as follows：It is a kind of at the same determine Lepidinm meyenii Walp in 11 kinds of macamide contents method, including with
Under several steps：
(1) preparation of solution to be measured
Crush, sieve, precision weighs maca powder, with 1g:10mL solid-liquid ratios add petroleum ether, supersound extraction 30min, mistake
Filter, filtering residue repeat extraction twice, merging filtrate, concentration, and solution to be measured is obtained with methanol constant volume；
(2) preparation of reference substance mixed liquor
Precision weighs N- (m- methoxy-benzyl)-linolenamide, N- benzyl linolenamide, N- (meta-methoxy benzyls respectively
Base)-sub- oleamide, N- benzyls Asia oleamide, N- benzyl pentadecanoyl amine, N- (m- methoxy-benzyl)-palmitoyl amine, N-
Benzyl palmitic amide, N- benzyl -9Z- octadecenamides, N- benzyl heptadecane amide, N- (m- methoxy-benzyl)-ten eight
Phosphoamide, N- benzyl kemanide S reference substances, mix after being dissolved with methanol respectively, are configured to multigroup reference substance mixed liquor, institute
The mass concentration of each reference substance in reference substance mixed liquor is stated in the range of 0.5～1000 μ g/mL；
(3) foundation of standard curve
Multigroup reference substance mixed solution in aspiration step (2), carries out liquid chromatogram measuring respectively；
Chromatographic condition is：Chromatographic column Xtimate XB-C18 4.6 × 250mm of chromatographic column, 5 μm；Mobile phase：Mobile phase A is
Water, Mobile phase B are acetonitrile；Gradient：0～24min, 80% Mobile phase B～100% Mobile phase B；Detector G4212-60008 bis-
Pole pipe array detector, Detection wavelength 210nm, 280nm；Flow velocity 0.8mL/min；40 DEG C of column temperature；10 μ L of sample size；
With in step (2), as abscissa, peak area does standard curve for vertical coordinate to the mass concentration of each reference substance；
(4) in solution to be measured macamide content measure
Solution to be measured is measured by the chromatographic condition of step (3), and calculates every in testing sample using standard curve
Plant the content of macamide.
Further, in the multigroup reference substance mixed solution described in step (3), the concentration range of each macamide is distinguished
For:0.5～35 μ g/mL, N- benzyls linolenamide of N- (m- methoxy-benzyl)-linolenamide, 5～300 μ g/mL, N- (methoxy
Base benzyl) 4～1000 μ g/mL, N- benzyl pentadecanoyl amine 1 of-sub- 0.5～100 μ g/mL, N- benzyl of oleamide Asia oleamide～
30 μ g/mL, N- (m- methoxy-benzyl)-palmitoyl amine, 5～50 μ g/mL, N- benzyls palmitic amide, 1～1000 μ g/mL,
5～150 μ g/mL, N- benzyl heptadecanes amide of N- benzyl -9Z- octadecenamides, 1～40 μ g/mL, N- (m- methoxybenzyl
Base) 1～10 μ g/mL, N- benzyls kemanide S of-octadecanoyl amine, 5～60 μ g/mL.
Further, the method is as follows to the detection limit of 11 kinds of macamides：
N- (meta-methoxy benzyl)-linolenamide：2.57；
N- benzyl linolenamide：1.46；
N- (meta-methoxy benzyl)-Asia oleamide：2.06；
N- benzyls Asia oleamide：1.64；
N- benzyl pentadecanoyl amine：10.35；
N- (meta-methoxy benzyl)-palmitoyl amine：2.91；
N- benzyl palmitic amides：2.82；
N- benzyl -9Z- octadecenamides：1.29；
N- benzyl heptadecane amide：7.85；
N- (meta-methoxy benzyl)-octadecanoyl amine：4.93；
N- benzyl kemanide Ss：3.41.
Beneficial effects of the present invention are as follows：
(1) extracting method of the invention, solves troublesome poeration, the extraction ratio of the methods such as existing backflow or surname extraction
Low, high cost, extraction time longer problem；
(2) detection method of the invention is gradient elution method, and separating degree is good, can detect 11 kinds of Lepidinm meyenii Walp in Lepidinm meyenii Walp simultaneously
Amide；The present invention only uses acetonitrile and water as mobile phase, solves trifluoroacetic acid, cleaning trouble big to chromatographic column loss etc. and asks
Topic, flowing phase configuration are convenient, easy operation；
(3) method of the present invention linear relationship is good, precision is high, reproducible, good stability, the response rate are high, it is easy,
It is quick and accurately feasible, can be used as the quantitative detecting method of main macamide standard in Lepidinm meyenii Walp.
Description of the drawings
Fig. 1 is the liquid chromatogram of macamide reference substance mixed liquor in embodiment 1；
Fig. 2 is the liquid chromatogram of S18 Lhasa purple Lepidinm meyenii Walp sample in embodiment 1.
Wherein：1 for numbering C1,2 for numbering C2,3 for numbering C3,4 for numbering C4,5 for numbering C5,6 for numbering C6,7 is
It is that numbering C10,11 is numbering C11 for numbering C9,10 that numbering C7,8 is numbering C8,9.
Specific embodiment
Technical scheme is further described with reference to specific embodiment, but the present invention is not with any shape
Formula is limited to embodiment content.Test method described in embodiment if no special instructions, is conventional method；Such as without special theory
Bright, the reagent and biomaterial are commercially obtained.
Embodiment 1
The present embodiment selects 20 kinds of Lepidinm meyenii Walp that numbering is S1-S20 to be measured as testing sample, comprises the following steps that：
(1) preparation of solution to be measured
Testing sample is crushed, is sieved, precision weighs 5.0g maca powders, add 50mL petroleum ether, supersound extraction 30min,
Filter, filtering residue method described above is extracted twice again, merging filtrate, concentration, with methanol constant volume to 10mL, treats HPLC quantitative analyses.
(2) preparation of reference substance mixed liquor
Precision weighs N- (m- methoxy-benzyl)-linolenamide, N- benzyl linolenamide, N- (meta-methoxy benzyls respectively
Base)-sub- oleamide, N- benzyls Asia oleamide, N- benzyl pentadecanoyl amine, N- (m- methoxy-benzyl)-palmitoyl amine, N-
Benzyl palmitic amide, N- benzyl -9Z- octadecenamides, N- benzyl heptadecane amide, N- (m- methoxy-benzyl)-ten eight
Phosphoamide, N- benzyl kemanide S reference substances, mix after being dissolved with methanol respectively, are configured to six groups of reference substance mixed liquors, institute
The mass concentration of each reference substance in reference substance mixed liquor is stated in the range of 0.5～1000 μ g/mL；The concentration of each reference substance is concrete
Such as table 1 below：
Table 1
(3) foundation of standard curve
Multigroup reference substance mixed solution in aspiration step (2), carries out liquid chromatogram measuring respectively；
Chromatographic condition is：Chromatographic column Xtimate XB-C18 4.6 × 250mm of chromatographic column, 5 μm；Mobile phase：Mobile phase A is
Water and Mobile phase B are acetonitrile；Gradient：0～24min, 80% Mobile phase B～100% Mobile phase B；Detector G4212-60008 bis-
Pole pipe array detector, Detection wavelength 210nm, 280nm；Flow velocity 0.8mL/min；40 DEG C of column temperature；10 μ L of sample size；
With in step (2), as abscissa, peak area does standard curve for vertical coordinate to the mass concentration of each reference substance；By noise
Than 3:1 determines that test limit, calibration curve equation and detection limit are shown in Table 2；
2 11 kinds of macamide reference substance calibration curve equations of table
(4) in solution to be measured macamide content measure
Chromatographic condition is：Chromatographic column Xtimate XB-C18 chromatographic columns (4.6 × 250mm, 5 μm)；Mobile phase be A (water) and
B (acetonitrile), gradient：0～24min, 80%B～100%B；Detector G4212-60008 diode array detector, detects ripple
Long 210nm, 280nm；Flow velocity 0.8mL/min；40 DEG C of column temperature；10 μ L of sample size, and calculated in testing sample using standard curve
The content of every kind of macamide.As a result such as table 3 below：
Content (the unit of macamide in 3 Lepidinm meyenii Walp of table：μg/g)
Embodiment 2
(1) Precision Experiment
Macamide reference substance solution is taken, above-mentioned chromatographic condition continuous sample introduction 3 times calculates the phase of each macamide peak area
To standard deviation (Relative Standard Deviation, RSD), as a result the RSD values of all macamides 0.94%～
Between 4.73%, show that instrument precision is good.As a result it is as shown in table 4.
4 Precision Experiment result (n=3) of table
(2) repeated experiment
Take S18 samples (originating in the purple Lepidinm meyenii Walp of Lhasa) and extract solution, above-mentioned chromatographic condition continuous sample introduction 3 times calculates each agate
The relative standard deviation of coffee amide content, as a result the RSD values of all macamides show the party between 0.42%～4.42%
Method repeatability is good.As a result it is as shown in table 5.
5 repeated experiment result (n=3) of table
(3) stability experiment
Same Lepidinm meyenii Walp sample extracting solution is taken, by above-mentioned chromatographic condition, sample introduction is analyzed after placing 0,2,4,8 and 12h respectively,
Content is calculated, the RSD of each macamide of sample is calculated, as a result its RSD value shows that test sample is molten between 0.47%～4.40%
Liquid is placed 12h at ambient temperature and is had good stability.As a result it is as shown in table 6.
6 stability experiment result (n=5) of table
(4) average recovery experiment
The Lepidinm meyenii Walp sample for taking known each macamide content of 9 parts of identical weights respectively prepares macamide as stated above
Extracting solution, is separately added into the macamide reference substance solution of different content, through 0.45 μm of filtering with microporous membrane after mixing completely,
10 μ L of sample introduction.The average recovery rate of each macamide shows that method accuracy rate is high, as a result between 98.23%～100.82%
It is shown in Table 7.
7 average recovery experimental result (n=9) of table
In sum, method of the present invention linear relationship is good, precision is high, reproducible, good stability, the response rate
It is high, easy, quick and accurately feasible, can be used as the quantitative detecting method of main macamide standard in Lepidinm meyenii Walp.
Claims (3)
1. a kind of at the same determine Lepidinm meyenii Walp in 11 kinds of macamide contents method, it is characterised in that including following step：
(1) preparation of solution to be measured
Crush, sieve, precision weighs maca powder, with 1g:10mL solid-liquid ratios add petroleum ether, supersound extraction 30min to filter,
Filtering residue repeats extraction twice, merging filtrate, concentration, and solution to be measured is obtained with methanol constant volume；
(2) preparation of reference substance mixed liquor
Precision weighs N- (m- methoxy-benzyl)-linolenamide, N- benzyl linolenamide, N- (meta-methoxy benzyl)-Asia respectively
Oleamide, N- benzyls Asia oleamide, N- benzyl pentadecanoyl amine, N- (m- methoxy-benzyl)-palmitoyl amine, N- benzyls ten
Six alkane amide, N- benzyl -9Z- octadecenamides, N- benzyl heptadecane amide, N- (m- methoxy-benzyl)-octadecanoyl
Amine, N- benzyl kemanide S reference substances, mix after being dissolved with methanol respectively, are configured to multigroup reference substance mixed liquor, described right
According to savoring the mass concentration of each reference substance in mixed liquor in the range of 0.5～1000 μ g/mL；
(3) foundation of standard curve
Multigroup reference substance mixed solution in aspiration step (2), carries out liquid chromatogram measuring respectively；
Chromatographic condition is：Chromatographic column Xtimate XB-C18 4.6 × 250mm of chromatographic column, 5 μm；Mobile phase：Mobile phase A is water, stream
Dynamic phase B is acetonitrile；Gradient：0～24min, 80% Mobile phase B～100% Mobile phase B；Detector G4212-60008 diode battle arrays
Row detector, Detection wavelength 210nm, 280nm；Flow velocity 0.8mL/min；40 DEG C of column temperature；10 μ L of sample size；
With in step (2), as abscissa, peak area does standard curve for vertical coordinate to the mass concentration of each reference substance；
(4) in solution to be measured macamide content measure
Solution to be measured is measured by the chromatographic condition of step (3), and every kind of agate in testing sample is calculated using standard curve
The content of coffee amide.
2. the method for determining 11 kinds of macamide contents in Lepidinm meyenii Walp according to claim 1 simultaneously, it is characterised in that step
(3) in the multigroup reference substance mixed solution described in, the concentration range of each macamide is respectively:N- (m- methoxy-benzyl)-
0.5～35 μ g/mL, N- benzyls linolenamide of linolenamide 5～300 μ g/mL, N- (meta-methoxy benzyl)-sub- oleamide 0.5～
4～1000 μ g/mL, N- benzyl pentadecanoyl amine of the sub- oleamide of 100 μ g/mL, N- benzyls, 1～30 μ g/mL, N- (m- methoxybenzyl
Base) 5～50 μ g/mL, N- benzyls palmitic amide of-palmitoyl amine, 1～1000 μ g/mL, N- benzyl -9Z- octadecenamides 5
～150 μ g/mL, N- benzyl heptadecanes amide 1～40 μ g/mL, N- (m- methoxy-benzyl) -1～10 μ g/mL of octadecanoyl amine,
5～60 μ g/mL of N- benzyls kemanide S.
3. the method for determining 11 kinds of macamide contents in Lepidinm meyenii Walp according to claim 1 simultaneously, it is characterised in that the method
It is as follows to the detection limit of 11 kinds of macamides：
N- (meta-methoxy benzyl)-linolenamide：2.57；
N- benzyl linolenamide：1.46；
N- (meta-methoxy benzyl)-Asia oleamide：2.06；
N- benzyls Asia oleamide：1.64；
N- benzyl pentadecanoyl amine：10.35；
N- (meta-methoxy benzyl)-palmitoyl amine：2.91；
N- benzyl palmitic amides：2.82；
N- benzyl -9Z- octadecenamides：1.29；
N- benzyl heptadecane amide：7.85；
N- (meta-methoxy benzyl)-octadecanoyl amine：4.93；
N- benzyl kemanide Ss：3.41.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611258172.4A CN106526033A (en) | 2016-12-30 | 2016-12-30 | Method for simultaneously testing 11 macamides content of maca |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611258172.4A CN106526033A (en) | 2016-12-30 | 2016-12-30 | Method for simultaneously testing 11 macamides content of maca |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106526033A true CN106526033A (en) | 2017-03-22 |
Family
ID=58336284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611258172.4A Pending CN106526033A (en) | 2016-12-30 | 2016-12-30 | Method for simultaneously testing 11 macamides content of maca |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106526033A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106974955A (en) * | 2017-04-12 | 2017-07-25 | 云南省药物研究所 | A kind of Maca extract for improving sex dysfunction and its preparation method and application |
| CN107748211A (en) * | 2017-09-14 | 2018-03-02 | 大连大学 | A kind of method using 5 kinds of macamides in deep co-melting solvent extraction measure maca |
| CN108535375A (en) * | 2018-03-26 | 2018-09-14 | 烟台新时代健康产业有限公司 | A method of based on the significant metabolite of LC-MS metabonomic analysis maca |
| CN110231428A (en) * | 2019-04-08 | 2019-09-13 | 国珍健康科技（北京）有限公司 | A kind of macamide detection method of content based on high performance liquid chromatography |
| CN110596264A (en) * | 2019-09-03 | 2019-12-20 | 谱尼测试集团股份有限公司 | Method for rapidly screening macamides compounds through UPLC-Q-TOF |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105866314A (en) * | 2016-05-31 | 2016-08-17 | 江苏慧博生物科技有限公司 | Detection method for analyzing content of macamides in Maca by virtue of HPLC |
-
2016
- 2016-12-30 CN CN201611258172.4A patent/CN106526033A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105866314A (en) * | 2016-05-31 | 2016-08-17 | 江苏慧博生物科技有限公司 | Detection method for analyzing content of macamides in Maca by virtue of HPLC |
Non-Patent Citations (3)
| Title |
|---|
| 舒积成等: "玛咖质量标准研究", 《中国中药杂志》 * |
| 邵金良等: "HPLC-PAD同时测定玛咖中8 种酰胺类化合物含量", 《江苏农业学报》 * |
| 黄云先等: "反相HPLC 法测定不同产地玛咖中玛咖酰胺的含量", 《中国保健营养》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106974955A (en) * | 2017-04-12 | 2017-07-25 | 云南省药物研究所 | A kind of Maca extract for improving sex dysfunction and its preparation method and application |
| CN107748211A (en) * | 2017-09-14 | 2018-03-02 | 大连大学 | A kind of method using 5 kinds of macamides in deep co-melting solvent extraction measure maca |
| CN107748211B (en) * | 2017-09-14 | 2020-05-19 | 大连大学 | Method for extracting and measuring 5 macamides in maca by using deep eutectic solvent |
| CN108535375A (en) * | 2018-03-26 | 2018-09-14 | 烟台新时代健康产业有限公司 | A method of based on the significant metabolite of LC-MS metabonomic analysis maca |
| CN108535375B (en) * | 2018-03-26 | 2020-08-04 | 烟台新时代健康产业有限公司 | Method for analyzing maca marked metabolites based on LC-MS (liquid chromatography-mass spectrometry) metabonomics |
| CN110231428A (en) * | 2019-04-08 | 2019-09-13 | 国珍健康科技（北京）有限公司 | A kind of macamide detection method of content based on high performance liquid chromatography |
| CN110596264A (en) * | 2019-09-03 | 2019-12-20 | 谱尼测试集团股份有限公司 | Method for rapidly screening macamides compounds through UPLC-Q-TOF |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106526033A (en) | Method for simultaneously testing 11 macamides content of maca | |
| CN105606734A (en) | Method for detecting honeysuckle flower and lonicerae flos medicinal materials through rapid resolution liquid chromatography | |
| CN107144661B (en) | Detection method that is a kind of while obtaining material composition and total antioxidant activity in Honeysuckle flower | |
| CN106290646B (en) | The detection method of Compound Jinyinhua Granules | |
| CN106124639A (en) | The multicomponent content assaying method of Eucommia ulmoides | |
| CN102175788A (en) | Method for detecting contents of citrinin in monascus by liquid chromatograph/mass spectrometer (LC/MS) | |
| CN104897787B (en) | A kind of method of six kinds of active component in NIUHUANG NINGGONG PIAN of mensuration simultaneously | |
| CN104777249A (en) | Method for determining content of effective ingredient amygdalin in loquat leaf cough syrup | |
| CN105699500A (en) | A method of measuring contents of seven components in a Huoxiangzhengqi dripping pill through ultra-high performance liquid chromatography | |
| CN107315058A (en) | A kind of method of total ginkgoic acid in detection ginkgo biloba succi | |
| CN108254471A (en) | The detection method of 5 hydroxymethyl furfural and furfural content in xylo-oligosaccharide | |
| CN104090040A (en) | HPLC method for rapidly detecting main active ingredients of lithocarpus polysachyus rehd | |
| CN106324167A (en) | Method for determining flavone components in radix astragali extract by UPLC (ultra performance liquid chromatography) | |
| CN106290603B (en) | A kind of method and application detecting inorganic anion in plant, organic acid and three kinds of phytochemicals simultaneously using Vavle switching method | |
| CN104634911B (en) | A kind of 4 kinds of flavonoids effective constituent detection methods of CHUANKEZHI ZHUSHEYE | |
| CN107748211B (en) | Method for extracting and measuring 5 macamides in maca by using deep eutectic solvent | |
| CN103344717A (en) | Method for establishing rhizoma bletillae high performance liquid chromatography (HPLC) fingerprint spectrum and standard fingerprint spectrum thereof | |
| CN107102078B (en) | A kind of method of aflatoxin B1 in measurement Gardenia Yellow | |
| CN106093264A (en) | A kind of assay method of Fructus Fragariae Ananssae Xanthophyll Cycle Components | |
| CN106324119B (en) | The UPLC method of 8 kinds of ingredients in lamiophlomis rotata medicinal material is measured simultaneously | |
| CN102335333A (en) | Method for detecting traditional Chinese medicine preparation of compound nutritions paste | |
| CN107102087A (en) | A kind of method of a variety of organic acid contents in chromatography of ions detection coptis | |
| CN103575823A (en) | Detection method of 8 chemical components in Tangminling preparation | |
| CN104133028B (en) | A kind of method for building up of madder granule efficient liquid-phase chromatograph finger print atlas | |
| CN108593794B (en) | Method for detecting content of effective components in safflower by using multi-index component UPLC |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170322 |
|
| RJ01 | Rejection of invention patent application after publication |