CN103304335A - Formula for agaricus blazei murill stock culture material and method for preparing culture material - Google Patents
Formula for agaricus blazei murill stock culture material and method for preparing culture material Download PDFInfo
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- CN103304335A CN103304335A CN2013102748188A CN201310274818A CN103304335A CN 103304335 A CN103304335 A CN 103304335A CN 2013102748188 A CN2013102748188 A CN 2013102748188A CN 201310274818 A CN201310274818 A CN 201310274818A CN 103304335 A CN103304335 A CN 103304335A
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- agaricus blazei
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to an agaricus blazei murill stock culture material. The agaricus blazei murill stock culture material is characterized in that rabbit manure pellets serve as raw materials, and agaricus blazei murill residues (mixtures of mushroom-bed fine soil and mushroom materials) serving as intergranular bridging materials are mixed with the rabbit manure pellets so as to prepare an agaricus blazei murill stock. The moisture content of the rabbit manure pellets is 45%, and the moisture content of the bridging materials is 65%. The invention further relates to a method for preparing the culture material. Compared with a wheat granule stock, the straw mushroom stock has the advantages that rabbit manure is comprehensive in nutrition, hyphae grow vigorously, and strains are powerful in vitality and are difficultly aged; due to the granular rabbit manure strains, during transfer cultivation, the strain consumption is low, and the work efficiency is high; in a sterilization link of strain preparation, pathogenic bacteria, parasitic ova and weed seeds in the rabbit manure are killed, so that the environment of a rabbit farm is cleaned; and the sources of the raw materials of the culture material are broadened, and the cost for stock preparation is reduced.
Description
Technical field
Content of the present invention comprises Agaricus blazei Murrill original seed culture material compatibility, and the making method of this culture material, belongs to edible fungus species and makes the field.
Background technology
Agaricus blazei Murrill has another name called Agaricus blazei, is a kind of rare edible mushrooms that eats the medicine dual-purpose.Agaricus blazei Murrill contains rich in protein, amino acid and polysaccharide, and the content of protein and polysaccharide is 2 times of mushroom, effect such as effective component has reducing blood-fat, antithrombotic, calm the nerves.Agaricus blazei Murrill is extremely welcome in Japan, Korea S and south east asia.
Along with the continuous expansion of Agaricus blazei Murrill cultivated area, the wheat consumption that the production of hybrid seeds is used also increases thereupon, for reducing grain consumption, must seek the reasonable substitute of the production of hybrid seeds.
China is a foster rabbit big country, also is rabbit products export big country (as world's rabbit hair volume of trade, China accounts for more than 90%).Along with the development of rabbit keeping, livestock on hand quantity and the sales volume of rabbit obviously increase, and are accompanied by movement-rabbit ight soil of rabbit also in corresponding increase.
Rabbit ight soil is as the pea size, and spherical in shape or oval, smooth in appearance is mellow and full, and loose even, fresh excreta is gloss slightly, is chocolate.It is reported that dried rabbit excrement contains crude protein 10.9%-12.7%, robust fibre 29.5%-35.9%, nitrogen-free extract 44.1%-47.8%, also contains various trace elements and VITAMIN.
Rabbit is herbivore, and feed is based on grass, so Mierocrystalline cellulose in the faecal pellet of rabbit, hemicellulose (robust fibre) content are higher, and Agaricus blazei Murrill cultivation material is excrement grass substratum, so the very suitable Agaricus blazei Murrill mycelia growth of rabbit excrement.
Utilize the rabbit faecal pellet to substitute wheat and make the Agaricus blazei Murrill original seed, be not only this behave of falling of an Edible Fungi, but also be a circular agriculture and environmental protection cause behave.
Summary of the invention
The present invention has following two aspect contents:
1, a kind of Agaricus blazei Murrill original seed culture material compatibility
2, the making method of culture material
The technical solution adopted in the present invention is:
A kind of Agaricus blazei Murrill original seed culture material compatibility has following component and quality proportioning: rabbit faecal pellet 85%-90%, Agaricus blazei slag (mixture of bacterium bed fine earth and bacterium material) 7%-12%, terra alba 2%, lime powder 1% all is the quality of dry-matter, and the proportioning sum is 100%.
Rabbit faecal pellet: fresh rabbit faecal pellet is standby after concrete floor dries.Nitrogenous (N) 2.3% of dried rabbit excrement, phosphorus (P
2O
3) 2.3%, potassium (K
2O) 0.8%.
The Agaricus blazei slag: get a foothold bacterium material of Agaricus blazei Murrill is ground into particle below the particle diameter 4mm, then with the Agaricus blazei bed on fine earth under the rake mix, dry standby.
Lime powder, terra alba: be common commercially available product.Regulate, cushion the potential of hydrogen of culture material.
The making method of culture material comprises the steps:
1. shine material: the 1d-2d that before spice raw material is exposed to the sun, utilize ultraviolet ray to kill the assorted bacterium of part in the raw material;
2. prewet: water is mixed the rabbit excrement wet before building heap, will there are flowing out on every side;
3. the fermentation of banking up: will mix wet rabbit excrement, pile high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the control of the culture material of every heap be at dry weight 250kg-500kg, carry out heap after, to pat all around, insert some vertical pores with the wooden stick of diameter 10cm-15cm at heap again, at the bottom of the straight through reactor, stomatal frequency is 1/m
3, cover straw mat at last by its spontaneous fermentation;
4. turning: after the material temperature reaches 60 ℃, keep 24h, carry out turning, admix auxiliary material (earlier Agaricus blazei slag, terra alba, lime powder being mixed thoroughly) then equably under drying regime, and look the suitable moisturizing of water content of windrow, turning is 1 time altogether, time remaining 3d;
When 5. stopping fermentation, the rabbit faecal pellet reaches no dry granular Bai Xinwu ammonia flavor, and water content was 45% (being puffy), and the water content of bacterium slag is 65%;
6. bottling: with the rabbit faecal pellet culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
7. sterilize, cool off: culture material is carried out autoclaving, keep 100 ℃ of sterilization 1h earlier, rise to 121 ℃ of autoclaving 2h then; Process of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting Agaricus blazei Murrill mother kind, and culturing room's temperature control is at 22 ℃-24 ℃, and relative air humidity is controlled at 60%-75%, and through the cultivation of 24d-25d, mycelia is covered with bottle, namely can be used as and makes the cultivar use.
The present invention is raw material with the rabbit faecal pellet, admixes Agaricus blazei slag (mixture of bacterium bed fine earth and bacterium material) as intergranular bridging material, makes the Agaricus blazei Murrill original seed.Compare with the wheat kind, its advantage is: the one, and rabbit excrement comprehensive nutrition, mycelial growth is vigorous, and spawn activity is strong, is difficult for aging; The 2nd, particulate state rabbit caprophyl kind, during the switching cultivar, sowing quantity is few, high efficiency; The 3rd, the sterilization link of the production of hybrid seeds has been killed pathogenic bacteria, parasitic ovum and weed seed in the rabbit excrement, has purified the hygienic condition in warren; The 4th, widened culture material raw material channel, reduced the bacterial classification cost of manufacture.
Embodiment
Mode 1: Agaricus blazei Murrill original seed culture material comprises following component and quality proportioning in the present embodiment: rabbit faecal pellet 85%, Agaricus blazei slag (mixture of bacterium bed fine earth and bacterium material) 12%, terra alba 2%, lime powder 1%, all be the quality of dry-matter, the proportioning sum is 100%.
Making method:
1. shine material: the 1d-2d that before spice raw material is exposed to the sun, utilize ultraviolet ray to kill the assorted bacterium of part in the raw material;
2. prewet: water is mixed the rabbit excrement wet before building heap, will there are flowing out on every side;
3. the fermentation of banking up: will mix wet rabbit excrement, pile high 1.0m, wide 2.5m, the trapezoidal heap of length 4m, carry out heap after, will around pat, insert some vertical pores with the wooden stick of diameter 10cm-15cm at heap again, at the bottom of the straight through reactor, stomatal frequency is 1/m
3, cover straw mat at last by its spontaneous fermentation;
4. turning: after the material temperature reaches 60 ℃, keep 24h, carry out turning, admix auxiliary material (earlier Agaricus blazei slag, terra alba, lime powder being mixed thoroughly) then equably under drying regime, and look the suitable moisturizing of water content of windrow, turning is 1 time altogether, time remaining 3d;
When 5. stopping fermentation, the rabbit faecal pellet reaches no dry granular Bai Xinwu ammonia flavor, and water content was 45% (being puffy), and the water content of bacterium slag is 65%;
6. bottling: with the rabbit faecal pellet culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
7. sterilize, cool off: culture material is carried out autoclaving, keep 100 ℃ of sterilization 1h earlier, rise to 121 ℃ of autoclaving 2h then; Process of cooling is: naturally cool to below 25 ℃.
Culture material is after inserting Agaricus blazei Murrill mother kind, and culturing room's temperature control is at 22 ℃-24 ℃, and relative air humidity is controlled at 60%-75%, and through the cultivation of 24d, mycelia is covered with bottle, namely can be used as and makes the cultivar use.
Mode 2: Agaricus blazei Murrill original seed culture material comprises following component and quality proportioning in the present embodiment: rabbit faecal pellet 90%, Agaricus blazei slag (mixture of bacterium bed fine earth and bacterium material) 7%, terra alba 2%, lime powder 1%, all be the quality of dry-matter, the proportioning sum is 100%.Making method is with reference to mode 1.
Mode 3 (control group): Agaricus blazei Murrill wheat kind culture material: wheat 97%, terra alba 3%.
By following table as can be known, the sprout time of three kinds of mode Agaricus blazei Murrill mycelia is 24h, and mycelial growth is dense, pure white, sturdy, and the full bottle of rabbit faecal pellet culture material time ratio control group shortens 5d-6d; From digestion time, the original seed that utilizes the rabbit faecal pellet to make is difficult for aging, and digestion time prolongs 8d than control group, can satisfy to prolong cultivar production to the time requirement of original seed.
Table three group culture material is to the influence of Agaricus blazei Murrill original seed mycelial growth
| Mode | Sprout time (h) | Mycelial density | Healthy and strong degree | The full bottle time (d) | Digestion time (d) |
| 1 | 24 | ++++ | Pure white, sturdy | 24 | 68 |
| 2 | 24 | ++++ | Pure white, sturdy | 25 | 68 |
| 3 | 24 | ++++ | Pure white, sturdy | 30 | 60 |
Annotate: mycelial density with ++, +++and ++ ++ expression ,+number more multilist shows that mycelial density is more high.
Claims (5)
1. an Agaricus blazei Murrill original seed culture material compatibility is characterized in that containing following component and quality proportioning: rabbit faecal pellet 85%-90%, Agaricus blazei slag (mixture of bacterium bed fine earth and bacterium material) 7%-12%, terra alba 2%, lime powder 1% all is the quality of dry-matter, and the proportioning sum is 100%.
2. Agaricus blazei Murrill original seed culture material according to claim 1 is characterized in that described rabbit faecal pellet is the argol grain after fresh rabbit excrement dries.
3. Agaricus blazei Murrill original seed culture material according to claim 1, it is characterized in that described Agaricus blazei slag be bacterium bed fine earth and bacterium material mixed dry material (the Agaricus blazei Murrill bacterium material of getting a foothold is ground into particle below the particle diameter 4mm, then with the Agaricus blazei bed on fine earth under the rake mix).
4. the making method of the Agaricus blazei Murrill original seed culture material described in the claim 1-3 is characterized in that:
1. shine material: the 1d-2d that before spice raw material is exposed to the sun, utilize ultraviolet ray to kill the assorted bacterium of part in the raw material;
2. prewet: water is mixed the rabbit excrement wet before building heap, will there are flowing out on every side;
3. the fermentation of banking up: will mix wet rabbit excrement, pile high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the control of the culture material of every heap be at dry weight 250kg-500kg, carry out heap after, to pat all around, insert some vertical pores with the wooden stick of diameter 10cm-15cm at heap again, at the bottom of the straight through reactor, stomatal frequency is 1/m
3, cover straw mat at last by its spontaneous fermentation;
4. turning: after the material temperature reaches 60 ℃, keep 24h, carry out turning, admix auxiliary material (earlier Agaricus blazei slag, terra alba, lime powder being mixed thoroughly) then equably under drying regime, and look the suitable moisturizing of water content of windrow, turning is 1 time altogether, time remaining 3d;
When 5. stopping fermentation, the rabbit faecal pellet reaches no dry granular Bai Xinwu ammonia flavor, and water content was 45% (being puffy), and the water content of bacterium slag is 65%;
6. bottling: with the rabbit faecal pellet culture material 1100ml * 78mmPP culturing bottle (do not press during bottling, be state of nature and get final product) of packing into, cover the breathe freely bottle cap of sponge of band then;
7. sterilize, cool off: culture material is carried out autoclaving, keep 100 ℃ of sterilization 1h earlier, rise to 121 ℃ of autoclaving 2h then; Process of cooling is: naturally cool to below 25 ℃.
5. utilize the making method of the Agaricus blazei Murrill original seed culture material described in the claim 1-4, after it is characterized in that culture material inserts female the kind, culturing room's temperature control is at 22 ℃-24 ℃, relative air humidity control is at 60%-75%, cultivation through 24d-25d, mycelia is covered with bottle, namely can be used as to make the cultivar use.
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|---|---|---|---|
| CN2013102748188A CN103304335A (en) | 2013-06-23 | 2013-06-23 | Formula for agaricus blazei murill stock culture material and method for preparing culture material |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103601590A (en) * | 2013-11-25 | 2014-02-26 | 湖南国进食用菌开发有限公司 | Culture medium of pleurotus cornucopiae, preparation of culture medium, and culture method of pleurotus cornucopiae |
| CN106242682A (en) * | 2016-08-04 | 2016-12-21 | 贵州宏华农业发展有限公司 | Agaricus blazei Murrill Primary spawn material and manufacture method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101885634A (en) * | 2009-05-12 | 2010-11-17 | 黄刚 | Agaricus blazei culture material |
| KR20110004760A (en) * | 2009-07-08 | 2011-01-14 | 오세권 | Manufacturing method of mushroom mycelium using sprout vegetable and young leaf vegetable |
| CN102301910A (en) * | 2011-06-16 | 2012-01-04 | 福建省农业科学院科技干部培训中心 | Method for cultivating high-quality Agaricus blazei murrill by utilizing Pleurotus eryngii waste fungi residues |
| CN103145462A (en) * | 2013-03-07 | 2013-06-12 | 邬金飞 | Enoki mushroom stock culture material and making method thereof |
-
2013
- 2013-06-23 CN CN2013102748188A patent/CN103304335A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101885634A (en) * | 2009-05-12 | 2010-11-17 | 黄刚 | Agaricus blazei culture material |
| KR20110004760A (en) * | 2009-07-08 | 2011-01-14 | 오세권 | Manufacturing method of mushroom mycelium using sprout vegetable and young leaf vegetable |
| CN102301910A (en) * | 2011-06-16 | 2012-01-04 | 福建省农业科学院科技干部培训中心 | Method for cultivating high-quality Agaricus blazei murrill by utilizing Pleurotus eryngii waste fungi residues |
| CN103145462A (en) * | 2013-03-07 | 2013-06-12 | 邬金飞 | Enoki mushroom stock culture material and making method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 郑林华: "珍稀食用菌-姬松茸", 《农村实用科技信息》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103601590A (en) * | 2013-11-25 | 2014-02-26 | 湖南国进食用菌开发有限公司 | Culture medium of pleurotus cornucopiae, preparation of culture medium, and culture method of pleurotus cornucopiae |
| CN106242682A (en) * | 2016-08-04 | 2016-12-21 | 贵州宏华农业发展有限公司 | Agaricus blazei Murrill Primary spawn material and manufacture method |
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Application publication date: 20130918 |