CN103304296A - Selenium-enriched organic fertilizer of tobacco stem - Google Patents
Selenium-enriched organic fertilizer of tobacco stem Download PDFInfo
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- CN103304296A CN103304296A CN2012100809939A CN201210080993A CN103304296A CN 103304296 A CN103304296 A CN 103304296A CN 2012100809939 A CN2012100809939 A CN 2012100809939A CN 201210080993 A CN201210080993 A CN 201210080993A CN 103304296 A CN103304296 A CN 103304296A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a selenium-enriched organic fertilizer of a tobacco stem. The selenium-enriched organic fertilizer is characterized by comprising the following operation steps of: a, preparing raw materials; b, fermenting; and c, carrying out drying treatment. The selenium-enriched organic fertilizer of the tobacco stem disclosed by the invention utilizes the tobacco stem, activated selenium ore powder, fulvic acid and urea to simply produce the selenium-enriched organic fertilizer, and effectively solves the reutilization problem of the tobacco stems. The technical keys are that the selenium ore powder which is crushed until the grain diameter is less than 0.15mm and and a fulvic acid solution with the pH (Potential of Hydrogen) value which is adjusted to 8.5 are utilized and tobacco stem powder is used as a raw material; and the urea and self-made zymophyte liquid are matched to produce the selenium-enriched organic fertilizer.
Description
Technical field
The present invention relates to a kind of tobacco rod selenium-enriched organic fertilizer.
Background technology
China's selenium total resources is abundant, possesses industrial reserves and occupies the 4th in the world.But, because the selenium maldistribution of the resources is even, China has 72% area to be in to lack among selenium and the low selenium ecotope, wherein tiltedly wear to the Yunnan-Guizhou Plateau from the Three Eastern Provinces, there is 2/3 regional staple crops selenium content to be lower than 0.05mg/kg approximately, is internationally recognized scarce selenium area.China's tobacco planting area was 1,858 ten thousand mu in 2011, wherein the scarce selenium of most cigarette district's soil.Smoking is harmful to your health, improves the content that the tobacco leaf selenium content can effectively reduce main harm compositions such as coke tar in cigarette toxicity and free radical, alleviates smoking to a certain extent to the injury of human body.Therefore, produce rich selenium tobacco leaf and have important economic value and social benefit.
Bestow favour, be known as " world's selenium is all ", the selenium resource reserve is abundant, the unique independent selenium mineral deposit that has the whole world up to now to find within the border, and containing around the selenium mineral deposit has more than one hundred million tons of selenium contents to contain the selenium coal gangue at 180-500mg/kg.Simultaneously, cultivated area and the output of China's tobacco plant all occupy first place in the world, more than 450 ten thousand tons of tobacco leaf annual production, corresponding more than 450 ten thousand tons of the output tobacco rods of wanting.Tobacco rod is not suitable for burning, and also fertilizer is made in not perishable also field, and a large amount of tobacco rods are dropped or incinerate, serious resource waste and pollution atmospheric environment.Therefore, reinforcement is to the tobacco rod comprehensive utilization of resources, realize the combination of selenium mineral powder and tobacco rod, not only can effectively handle these wastes, and can turn waste into wealth, improve soil physico-chemical property and improve the selenium content level, increase selenium content of crops, yield and quality, the scarce selenium district people are mended selenium have great importance.
The present invention is with reference to the relevant patent of domestic and international selenium-enriched organic fertilizer preparation.Though can produce selenium-enriched organic fertilizer, have all that raw material is various, a problem such as complex manufacturing, cost are higher.The present invention utilizes cigarette stalk, selenium mineral powder, xanthohumic acid and urea, easy production selenium-enriched organic fertilizer, and efficiently solve the recycling problem of cigarette stalk.Its key problem in technology is to utilize through being crushed to particle diameter to transfer to 8.5 xanthohumic acid solution less than the selenium mineral powder of 0.15mm and pH, is raw material with cigarette stalk powder, is equipped with urea and homemade zymocyte liquid, produces selenium-enriched organic fertilizer.The objective of the invention is to overcome existing preparation method's defective, provide that a kind of raw material is easy to get, selenium content with low cost, easy and simple to handle, effective high and environment amenable selenium-enriched organic fertilizer novel preparation method.Selenium-enriched organic fertilizer with present method production, selenium content is 61.8-103.0mg/kg, and selenium validity has improved 7.20%-9.66%, is applied to leaf tobacco production reality, the more common fertilizer of output has improved 0.9%-28.2%, and ripe tobacco leaf selenium content has improved 26.9%-36.3%.
Summary of the invention
The objective of the invention is to overcome existing preparation method's defective, provide that a kind of raw material is easy to get, selenium content with low cost, easy and simple to handle, effective high and environment amenable a kind of tobacco rod selenium-enriched organic fertilizer.
A kind of tobacco rod selenium-enriched organic fertilizer of the present invention is characterized in that comprising following operation steps:
A, preparation of raw material
Learning from else's experience is crushed to particle diameter less than the tobacco rod of 2mm, adds water and makes the tobacco rod moisture content reach 60%; Adding urea evenly mixes with tobacco rod; Get xanthohumic acid with water dissolution after, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Add selenium mineral powder again, evenly mix with tobacco rod;
B, fermentation
Be inoculated in raw material with the zymocyte liquid through activation culture; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again;
C, drying treatment
Fertilizer is carried out drying treatment, to water content be 17%-18%;
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg;
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively; The bacterial classification of selecting adopts the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; The 4 ℃ of stored refrigerated in zymophyte inclined-plane; During use, the activation zymophyte, the inoculation of be about to preserving to liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
A kind of tobacco rod selenium-enriched organic fertilizer of the present invention is characterized in that comprising following operation steps:
A, preparation of raw material
Learning from else's experience is crushed to particle diameter less than the tobacco rod of 2mm, adds water and makes the tobacco rod moisture content reach 60%; The urea that adds tobacco rod quality 2% evenly mixes with tobacco rod; Get tobacco rod quality 4% xanthohumic acid with 4 times of water dissolution after, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Claim tobacco rod quality 10%-45% selenium mineral powder again, evenly mix with tobacco rod;
B, fermentation
Zymocyte liquid with 1L process activation culture is inoculated in raw material; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again;
C, drying treatment
Fertilizer is carried out drying treatment, to water content be 17%-18%;
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg;
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively; The bacterial classification of selecting adopts the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; The 4 ℃ of stored refrigerated in zymophyte inclined-plane; During use, the activation zymophyte, the inoculation of be about to preserving to liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
A kind of tobacco rod selenium-enriched organic fertilizer of the present invention is characterized in that comprising following operation steps:
A, preparation of raw material
Learning from else's experience is crushed to particle diameter less than the tobacco rod 700kg of 2mm, adds water and makes the tobacco rod moisture content reach 60%; Adding 14kg urea evenly mixes with tobacco rod; Get the 28kg xanthohumic acid with 4 times of water dissolution after, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Claim the 300kg selenium mineral powder again, evenly mix with tobacco rod;
B, fermentation
Zymocyte liquid with 1L process activation culture is inoculated in raw material; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again;
C, drying treatment
Fertilizer is carried out drying treatment, to water content be 17%-18%;
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg;
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively, cultural method is respectively: it is extractum carnis 3g, peptone 10g, sodium-chlor 5g, cycloheximide 50mg, distilled water 1000ml that bacterium is selected substratum, dissolving back adjust pH 7.2-7.4,120 ℃ of sterilization 15min, the every 100ml substratum in cooling back adds sample vat liquor 0.5ml, at 37 ℃ with 120r/min shaking culture 24 hours; It is glucose 20g, peptone 10g, yeast extract paste 10g, distilled water 1000ml that yeast is selected substratum, dissolves back 120 ℃ of sterilization 15min, and the cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; It is Zulkovsky starch 20g, sodium-chlor 0.5g, saltpetre 1g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, ferrous sulfate 0.01g, distilled water 1000ml that actinomycetes are selected substratum, dissolving back adjust pH 7.2-7.4,120 ℃ of sterilization 15min, cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; It is ammonium sulfate 2g, sal epsom 0.5g, potassium primary phosphate 1g, sodium-chlor 0.5g, strip quantitative paper 15g, distilled water 1000ml that cellulose-decomposing bacterium is selected substratum, dissolve back 120 ℃ of sterilization 15min, cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; Zymophyte purification cultivation method: select substratum to add agar 20g for every kind, other composition is identical, dissolves back 120 ℃ of sterilization 15min, and the cooling back is fallen dull and stereotyped, adopt the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; Zymophyte inclined-plane store method: sodium-chlor 4.2g, potassium primary phosphate 1.0g, dipotassium hydrogen phosphate 3.1g, SODIUMNITRATE 3.0g, extractum carnis 3.0g, peptone 10.0g, yeast extract paste 1.5g, glucose 2.0g, sucrose 30.0g, agar 15g, distilled water 1000ml, dissolve back 120 ℃ of sterilization 15min, cooling back contra bevel, to inoculate through the various microorganisms of purification process, cultivate 12h, 4 ℃ of stored refrigerated for 37 ℃; Zymophyte activation method: sodium-chlor 4.2g, potassium primary phosphate 1.0g, dipotassium hydrogen phosphate 3.1g, SODIUMNITRATE 3.0g, extractum carnis 3.0g, peptone 10.0g, yeast extract paste 1.5g, glucose 2.0g, sucrose 30.0g, distilled water 1000ml, dissolve back 120 ℃ of sterilization 15min, after the cooling strain separated all is seeded in the liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
A kind of tobacco rod selenium-enriched organic fertilizer of the present invention is to utilize cigarette stalk, selenium mineral powder, xanthohumic acid and urea, easy production selenium-enriched organic fertilizer, and efficiently solve the recycling problem of cigarette stalk.Its key problem in technology is to utilize through being crushed to particle diameter to transfer to 8.5 xanthohumic acid solution less than the selenium mineral powder of 0.15mm and pH, is raw material with cigarette stalk powder, is equipped with urea and homemade zymocyte liquid, produces selenium-enriched organic fertilizer.According to the rule of cigarette strain absorbed nitrogen, phosphorus, potassium and selenium, carry out the rational proportion of nutrient, production technique is simple, contains nutritive elements such as abundant nitrogen, phosphorus, potassium, sulphur and selenium, molybdenum, particularly contains a large amount of active selenium compositions, is the complete fertilizer of a kind of nutrient.Tobacco rod selenium-enriched organic fertilizer with present method production, selenium content is 61.8-103.0mg/kg, and selenium validity has improved 7.20%-9.66%, is applied to leaf tobacco production reality, the more common fertilizer of output has improved 0.9%-28.2%, and ripe tobacco leaf selenium content has improved 26.9%-36.3%.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but the working of an invention mode is not limited thereto.
Embodiment 1
Claim the 700kg tobacco rod, add water, make the tobacco rod moisture content reach 60%; Getting 14kg urea evenly mixes with tobacco rod; Get the 28kg xanthohumic acid, after 4 times of water dissolution, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Get the 300kg selenium mineral powder, evenly mix with tobacco rod; Zymocyte liquid with 1L process activation culture is inoculated in raw material; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again; Fertilizer is carried out drying treatment, to water ratio be 17%-18%.Sample detection is packaged as the 40kg/ bag.
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg, water soluble selenium content is 37.4mg/kg.
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively, cultural method is respectively: it is extractum carnis 3g, peptone 10g, sodium-chlor 5g, cycloheximide 50mg, distilled water 1000ml that bacterium is selected substratum, dissolving back adjust pH 7.2-7.4,120 ℃ of sterilization 15min, the every 100ml substratum in cooling back adds sample vat liquor 0.5ml, at 37 ℃ with 120r/min shaking culture 24 hours; It is glucose 20g, peptone 10g, yeast extract paste 10g, distilled water 1000ml that yeast is selected substratum, dissolves back 120 ℃ of sterilization 15min, and the cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; It is Zulkovsky starch 20g, sodium-chlor 0.5g, saltpetre 1g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, ferrous sulfate 0.01g, distilled water 1000ml that actinomycetes are selected substratum, dissolving back adjust pH 7.2-7.4,120 ℃ of sterilization 15min, cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; It is ammonium sulfate 2g, sal epsom 0.5g, potassium primary phosphate 1g, sodium-chlor 0.5g, strip quantitative paper 15g, distilled water 1000ml that cellulose-decomposing bacterium is selected substratum, dissolve back 120 ℃ of sterilization 15min, cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; Zymophyte purification cultivation method: select substratum to add agar 20g for every kind, other composition is identical, dissolves back 120 ℃ of sterilization 15min, and the cooling back is fallen dull and stereotyped, adopt the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; Zymophyte inclined-plane store method: sodium-chlor 4.2g, potassium primary phosphate 1.0g, dipotassium hydrogen phosphate 3.1g, SODIUMNITRATE 3.0g, extractum carnis 3.0g, peptone 10.0g, yeast extract paste 1.5g, glucose 2.0g, sucrose 30.0g, agar 15g, distilled water 1000ml, dissolve back 120 ℃ of sterilization 15min, cooling back contra bevel, to inoculate through the various microorganisms of purification process, cultivate 12h, 4 ℃ of stored refrigerated for 37 ℃; Zymophyte activation method: sodium-chlor 4.2g, potassium primary phosphate 1.0g, dipotassium hydrogen phosphate 3.1g, SODIUMNITRATE 3.0g, extractum carnis 3.0g, peptone 10.0g, yeast extract paste 1.5g, glucose 2.0g, sucrose 30.0g, distilled water 1000ml, dissolve back 120 ℃ of sterilization 15min, after the cooling strain separated all is seeded in the liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
In the tobacco rod selenium-enriched organic fertilizer fermenting process, temperature has 6 days at the fate more than 50 ℃, and tobacco rod selenium-enriched organic fertilizer detected result sees Table 1 water soluble selenium ratio and reaches 26.5%, has improved 6.7% than selenium mineral powder.The fertilizer vat liquor is used for the Plantula Brassicae chinensis presprouting of seeds at 30 ℃, and percentage of germination was 90% in 24 hours.Fertilizer application is in leaf tobacco production, and per mu yield has improved 0.9%-15.2%, and a cigarette strain group phase, prosperous long-term, baking back middle leaf selenium content are respectively 0.146mg/kg, 0.124mg/kg and 0.608mg/kg, have improved 329.4%, 254.3% and 26.9% respectively.
Table 1: Se-enriched bio-organic fertilizer detected result
Embodiment 2
Claim the 900kg tobacco rod, add water, make the tobacco rod moisture content reach 60%; Getting 18kg urea evenly mixes with tobacco rod; Get the 36kg xanthohumic acid, after 4 times of water dissolution, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Get the 100kg selenium mineral powder, evenly mix with tobacco rod; Zymocyte liquid with 1L process activation culture is inoculated in raw material; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again; Fertilizer is carried out drying treatment, to water ratio be 17%-18%.Sample detection is packaged as the 40kg/ bag.
In the tobacco rod selenium-enriched organic fertilizer fermenting process, temperature has 8 days at the fate more than 50 ℃.Tobacco rod selenium-enriched organic fertilizer detected result sees Table 2 water soluble selenium ratios and has reached 29.5%, has improved 9.7% than selenium mineral powder.The fertilizer vat liquor is used for the Plantula Brassicae chinensis presprouting of seeds at 30 ℃, and percentage of germination was 100% in 24 hours.Selenium-enriched organic fertilizer is applied to leaf tobacco production, per mu yield has improved 11.1%-28.2%, a middle part tobacco leaf group phase, prosperous long-term, baking back tobacco leaf selenium content are respectively 0.111mg/kg, 0.050mg/kg and 0.653mg/kg, have improved 226.5%, 42.9% and 36.3% respectively.
Table 2: Se-enriched bio-organic fertilizer detected result
Illustrate: the hydride generation atomic fluorescence spectrographic determination (NY/T1104-2006) of the selenium in tobacco leaf and the fertilizer; The hydride generation atomic fluorescence spectrographic determination (NY/T1972-2010) of fertilizer water soluble selenium; Fertilizer with sulfuric acid-hydrogen peroxide disappear boil after, full nitrogen, full phosphorus and full potassium are used Kjeldahl determination, the yellow colorimetric method for determining of vanadium molybdenum and flame spectrophotometric determination (NY525-2002) respectively.
Claims (3)
1. tobacco rod selenium-enriched organic fertilizer is characterized in that comprising following operation steps:
A, preparation of raw material
Learning from else's experience is crushed to particle diameter less than the tobacco rod of 2mm, adds water and makes the tobacco rod moisture content reach 60%; Adding urea evenly mixes with tobacco rod; Get xanthohumic acid with water dissolution after, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Add selenium mineral powder again, evenly mix with tobacco rod;
B, fermentation
Be inoculated in raw material with the zymocyte liquid through activation culture; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again;
C, drying treatment
Fertilizer is carried out drying treatment, to water content be 17%-18%;
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg;
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively; The bacterial classification of selecting adopts the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; The 4 ℃ of stored refrigerated in zymophyte inclined-plane; During use, the activation zymophyte, the inoculation of be about to preserving to liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
2. according to the described tobacco rod selenium-enriched organic fertilizer of claim 1, it is characterized in that comprising following operation steps:
A, preparation of raw material
Learning from else's experience is crushed to particle diameter less than the tobacco rod of 2mm, adds water and makes the tobacco rod moisture content reach 60%; The urea that adds tobacco rod quality 2% evenly mixes with tobacco rod; Get tobacco rod quality 4% xanthohumic acid with 4 times of water dissolution after, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Claim tobacco rod quality 10%-45% selenium mineral powder again, evenly mix with tobacco rod;
B, fermentation
Zymocyte liquid with 1L process activation culture is inoculated in raw material; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again;
C, drying treatment
Fertilizer is carried out drying treatment, to water content be 17%-18%;
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg;
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively; The bacterial classification of selecting adopts the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; The 4 ℃ of stored refrigerated in zymophyte inclined-plane; During use, the activation zymophyte, the inoculation of be about to preserving to liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
3. according to the described tobacco rod selenium-enriched organic fertilizer of claim 1, it is characterized in that comprising following operation steps:
A, preparation of raw material
Learning from else's experience is crushed to particle diameter less than the tobacco rod 700kg of 2mm, adds water and makes the tobacco rod moisture content reach 60%; Adding 14kg urea evenly mixes with tobacco rod; Get the 28kg xanthohumic acid with 4 times of water dissolution after, with potassium hydroxide adjust pH to 8.5, evenly mix with tobacco rod; Claim the 300kg selenium mineral powder again, evenly mix with tobacco rod;
B, fermentation
Zymocyte liquid with 1L process activation culture is inoculated in raw material; To cover plastics film well through the stockpile of inoculation ferments; Turning was 1 time in per 2 days, turned over altogether 5 times, stacked fermentation 28 days again;
C, drying treatment
Fertilizer is carried out drying treatment, to water content be 17%-18%;
Described selenium mineral powder be with the selenium ore pulverize, ball milling, to particle diameter less than 0.15mm, make the selenium mineral powder that selenium content is not less than 180mg/kg;
Described zymocyte liquid through activation culture is that cigarette stalk, selenium ore, rotten wood, ight soil and soil 5 duplicate samples of gathering high selenium zone are carried out the selection cultivation of bacterium, yeast, actinomycetes, cellulose-decomposing bacterium respectively, cultural method is respectively: it is extractum carnis 3g, peptone 10g, sodium-chlor 5g, cycloheximide 50mg, distilled water 1000ml that bacterium is selected substratum, dissolving back adjust pH 7.2-7.4,120 ℃ of sterilization 15min, the every 100ml substratum in cooling back adds sample vat liquor 0.5ml, at 37 ℃ with 120r/min shaking culture 24 hours; It is glucose 20g, peptone 10g, yeast extract paste 10g, distilled water 1000ml that yeast is selected substratum, dissolves back 120 ℃ of sterilization 15min, and the cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; It is Zulkovsky starch 20g, sodium-chlor 0.5g, saltpetre 1g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, ferrous sulfate 0.01g, distilled water 1000ml that actinomycetes are selected substratum, dissolving back adjust pH 7.2-7.4,120 ℃ of sterilization 15min, cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; It is ammonium sulfate 2g, sal epsom 0.5g, potassium primary phosphate 1g, sodium-chlor 0.5g, strip quantitative paper 15g, distilled water 1000ml that cellulose-decomposing bacterium is selected substratum, dissolve back 120 ℃ of sterilization 15min, cooling back adds 0.5% sample vat liquor, at 37 ℃ with 120r/min shaking culture 24 hours; Zymophyte purification cultivation method: select substratum to add agar 20g for every kind, other composition is identical, dissolves back 120 ℃ of sterilization 15min, and the cooling back is fallen dull and stereotyped, adopt the plate streaking partition method to carry out purifying, according to morphological specificity and gramstaining evaluation identical bacterial classification is merged; Zymophyte inclined-plane store method: sodium-chlor 4.2g, potassium primary phosphate 1.0g, dipotassium hydrogen phosphate 3.1g, SODIUMNITRATE 3.0g, extractum carnis 3.0g, peptone 10.0g, yeast extract paste 1.5g, glucose 2.0g, sucrose 30.0g, agar 15g, distilled water 1000ml, dissolve back 120 ℃ of sterilization 15min, cooling back contra bevel, to inoculate through the various microorganisms of purification process, cultivate 12h, 4 ℃ of stored refrigerated for 37 ℃; Zymophyte activation method: sodium-chlor 4.2g, potassium primary phosphate 1.0g, dipotassium hydrogen phosphate 3.1g, SODIUMNITRATE 3.0g, extractum carnis 3.0g, peptone 10.0g, yeast extract paste 1.5g, glucose 2.0g, sucrose 30.0g, distilled water 1000ml, dissolve back 120 ℃ of sterilization 15min, after the cooling strain separated all is seeded in the liquid nutrient medium, at 37 ℃ with 120r/min shaking culture 24h.
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Cited By (3)
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| CN103936504A (en) * | 2014-04-10 | 2014-07-23 | 中国水稻研究所 | Preparation method of micro-nano mineral selenium fertilizer |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
| CN113277900A (en) * | 2021-06-29 | 2021-08-20 | 湖北中烟工业有限责任公司 | Production process of tobacco straw bio-organic fertilizer |
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| CN101125771A (en) * | 2006-09-21 | 2008-02-20 | 李树春 | Medical stone multifunctional high-calcium selenium silicon enriched fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103936504A (en) * | 2014-04-10 | 2014-07-23 | 中国水稻研究所 | Preparation method of micro-nano mineral selenium fertilizer |
| CN103936504B (en) * | 2014-04-10 | 2015-12-30 | 中国水稻研究所 | A kind of preparation method of micro-nano mineral selenium fertilizer |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
| CN113277900A (en) * | 2021-06-29 | 2021-08-20 | 湖北中烟工业有限责任公司 | Production process of tobacco straw bio-organic fertilizer |
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