CN103301338A - Traditional Chinese medicine preparation for treating hemophilia and preparation method thereof - Google Patents
Traditional Chinese medicine preparation for treating hemophilia and preparation method thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine preparation for treating hemophilia and a preparation method thereof. The traditional Chinese medicine is prepared from the following crude drugs of water chestnut, radix rubiae, buffalo horn, astragalus membranaceus, chickweed, codonopsis pilosula, hairyvein agrimony, herba violae, subprostrate sophora, celastrus aculeatus, dried rehamnnia root, fragrant hair grass, lalang grass rhizome, radix ranunculi ternate, placenta hominis, antelope horn, sweet wormwood, receptaculum nelumbinis, rhizoma bletillae, broken dense flowers and liquorice. The traditional Chinese medicine preparation has the beneficial effects that the traditional Chinese medicine preparation is a pure traditional Chinese medicine preparation, free of toxic and side effects, accurate in effect of bleeding pain, fast to become effective, good in treatment effect, convenient to take, available in materials, and low in drug cost, does not generate the drug dependence after being taken for a long period of time, and can help a sufferer get rid of whole blood and blood coagulation factors; the economical burden on treatment of the sufferer is reduced.
Description
Technical field
The present invention relates to pharmaceutical technology, relate in particular to a kind of haemophiliachemophiliac Chinese medicine preparation and preparation method for the treatment of.
Background technology
Hemophilia is one group of hemorrhage that hereditary coagulation factors deficiency causes.Thrombin is that interior one group of human body has the biological activity protein that causes blood coagulation, has hemostatic function, and main thrombin has 13 kinds, and Roman number commonly used is expressed as: I, II....XIII (being thrombin one, two ... to 13).If lack a certain thrombin in the blood, blood just is not easy to solidify, thus causing bleeding property disease.In China, haemophiliachemophiliac social man group's sickness rate is 5～10,/10 ten thousand, baby's incidence rate approximately 1/5000.The classical haemophilia patient often coagulation disorders occurs, hemorrhage can not spontaneously stopping after morbidity childhood, spontaneous or slight wound; Thereby at wound, the often haemophilia of performing the operation, but severe patient also hematostaxis after than aggravating activities.Primary disease is mainly hereditary, and doctor trained in Western medicine there is no radical treatment at present, and the base therapy measure is alternative medicine, and other drug all has temporary transient anastalsis.
Hemophilia can be divided into according to its difference that lacks the thrombin kind:
(1). hemophilia A: being because thrombin eight (being VIII) lacks and to cause, also being called hemophilia A, is modal hemophilia clinically, accounts for the 80%-85% of hemophilia number, in some hotspot even higher.
(2). hemophilia B: be because thrombin nine (being IX) shortage causes, also be called the hemophilia B, clinical rare than hemophilia A, account for about 15% of hemophilia number.
(3). hemophilia C: lack thrombin 11 (be that XI lacks, be called again the Rosenthal syndrome abroad).The XI deficiency disease is extremely rare in China.
(4). acquired Hemophilia (being the posteriority deficiency of coagulation factors): normal because oneself factor causes some thrombin level to descend, or activity decreased, such as acquired thrombin eight (VIII) deficiency disease, it is normal because self produces the VIII factor antibody, cause coagulation disorders, cause acquired Hemophilia (hemophilia A).
Though without the title of " hemophilia ", surely belong to " blood disorder " or " disorders of blood " scope according to haemophiliachemophiliac syndrome in motherland's traditional Chinese medical science medical science.From " interior warp ", ancient Chinese medicine doctor has more detailed elaboration to syndrome, etiology and pathogenesis, Therapeutic Principle and the square medicine of blood disorder.
Ming Dynasty Zhang Jiebin Jing-Yue Complete Works is discussed very detailed to blood disorder; " opinion is controlled blood disorder, must know that it wants, and blood moving by fire only, gas only.Examine fiery person, fire is arranged without fire but examine it.The person that examines gas, but examine its deficiency of vital energy QI excess." Ming Dynasty's Zhao Xianke " Thorough Knowledge of Medicine " thinks dry such as true water in the kidney, then kidney-yangly certainly will go up inflammation, blood also seethes with excitement with fire; Such as kidney-yang depletion in the kidney, then true water is counter contains, and blood is also become homeless and depended on and upper general, and this is two kinds of pathological changes of blood disorder.Ming Dynasty's tassel Zhong Chun " Medical Notes in Xianxing Study" " has further proposed to control haematemesis three and has wanted method: " should promoting the circulation of blood should not stop blooding being suitable to invigorate the liver and not to reduce it, the unsuitable pathogenic fire reducing of suitable sending down the abnormal ascending QI ".
" blood does not have opinion " in Qing Dynasty Tang Rong river proposed to control blood four methods: take hemostasis as first method, repercussive is the second method, and calming blood is the three therapeutic methods of traditional Chinese medicine, and tonify deficiency is for receiving the method for merit.
The traditional Chinese medical science thinks that the kidney being the origin of congenital constitution, and main bone is given birth to marrow, store essential substances, and essence can be changed hemopoietic liquid.Therefore the kidney invigorating should be prosecuted to the end in the haemophiliachemophiliac process for the treatment of, regulates the real basic principle for the treatment primary disease of equilibrium between yin and yang of kidney.
Haemophiliachemophiliac clinical manifestation mainly contains the following aspects:
(1) skin, mucosal bleeding: be hemorrhage multiple position.As seen nasal bleeding is arranged, the gums oozing of blood.Hemorrhage or hematoma arranged after the collision of the common forehead of child, the dermal ecchymosis, petechia are rare.Postextraction hemorrhage often is the reason of going to a doctor incessantly.
(2) arthrorrhagia: be one of feature of primary disease.Showing as the interior periarticular that reaches of articular cavity organizes hemorrhage.Be more common in the Medium or severe type patient.Often occur in wound, walked for a long time, after the strenuous exercise.Secondly knee joint and the ankle joint of invading are hip, elbow, shoulder, wrist and spinal column more.Arthrorrhagia was divided into for three phases: first phase is acute stage, and main manifestations is the red and swollen heat pain in joint; Second phase is the panarthritis phase, is that blood absorption is incomplete owing to Repeated Hemorrhage, forms chronic inflammatory disease, and synovial membrane thickens; Three phases were the later stage, joint fibrosis or ankylosis, deformity, amyotrophy.Bone destruction, arthrogryposis be so that the function of joint forfeiture, the knee joint flexing occurs, turn up, fibula is the subluxation shape.
(3) hemorrhage of muscle: be one of the most outstanding performance of hemophilia, all can occur everywhere at whole body.Can form hematoma when deep part muscle is hemorrhage, partial gall, limitation of activity can appear in the hematoma compression peripheral nerve.The hematoma compression blood vessel then causes the local blood circulation obstacle, causes ischemic injury, fiber to change, and gangrene can appear in severe patient.
(4) hematuria: gross hematuria or microscopic hematuria can appear in heavy hemophiliac, and be how painless, without trauma history, if there is the ureter clot to form, often with renal colic.
(5) other positions are hemorrhage: if hematemesis is arranged, the companion stomachache of having blood in stool, be digestive tract hemorrhage; If hemoptysis, spitting of blood, hemothorax are that lung and breast abdomen are hemorrhage, more rare; Intracranial hemorrhage is less, and the after craniocerebral injury of being everlasting occurs, and prognosis is not good, often is the cause of death.
Summary of the invention
Technical problem to be solved by this invention is, a kind of haemophiliachemophiliac Chinese medicine preparation and preparation method of being used for the treatment of is provided, Chinese medicine have determined curative effect, rapid-action, safe, have no side effect and the simple advantage of medication.
In order to solve the problems of the technologies described above, the invention provides a kind of haemophiliachemophiliac Chinese medicine preparation for the treatment of, comprise following raw medicinal material: Corm Eleocharitis, Radix Rubiae, Cornu Bubali, the Radix Astragali, Herba stellariae mediae, Radix Codonopsis, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, Radix Rehmanniae, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae, Herba Artemisiae Annuae, Receptaculum Nelumbinis, Pseudobulbus Bletillae (Rhizoma Bletillae), broken close flower and Radix Glycyrrhizae.
Wherein, the ratio of weight and number of each raw medicinal material can be Corm Eleocharitis 10～15 weight portions in the described Chinese medicine preparation, Radix Rubiae 20～40 weight portions, Cornu Bubali 10～25 weight portions, the Radix Astragali 15～30 weight portions, Herba stellariae mediae 20～40 weight portions, Radix Codonopsis 20～30 weight portions, Herba Agrimoniae 15～30 weight portions, Herba Violae 20～35 weight portions, Radix Sophorae Tonkinensis 6～12 weight portions, Celastrus aceleatus Merr 15～25 weight portions, Radix Rehmanniae 20～30 weight portions, Rhizoma seu Herba Valerianae Tanguticae 10～20 weight portions, Rhizoma Imperatae 15～20 weight portions, Radix Ranunculi Ternati 10～20 weight portions, Placenta Hominis 20～30 weight portions, Cornu Saigae Tataricae 20～40 weight portions, Herba Artemisiae Annuae 10～30 weight portions, Receptaculum Nelumbinis 10～20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 20～30 weight portions, broken close 20～40 weight portions and Radix Glycyrrhizae 20～40 weight portions spent.
Wherein, the ratio of weight and number of each raw medicinal material can also be Corm Eleocharitis 12～15 weight portions in the described Chinese medicine preparation, Radix Rubiae 20～30 weight portions, Cornu Bubali 10～20 weight portions, the Radix Astragali 15～20 weight portions, Herba stellariae mediae 20～30 weight portions, Radix Codonopsis 20～25 weight portions, Herba Agrimoniae 20～30 weight portions, Herba Violae 20～30 weight portions, Radix Sophorae Tonkinensis 6～10 weight portions, Celastrus aceleatus Merr 20～25 weight portions, Radix Rehmanniae 20～25 weight portions, Rhizoma seu Herba Valerianae Tanguticae 16～20 weight portions, Rhizoma Imperatae 18～20 weight portions, Radix Ranunculi Ternati 10～16 weight portions, Placenta Hominis 20～26 weight portions, Cornu Saigae Tataricae 20～30 weight portions, Herba Artemisiae Annuae 20～30 weight portions, Receptaculum Nelumbinis 15～20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 20～25 weight portions, broken close 25～35 weight portions and Radix Glycyrrhizae 30～40 weight portions spent.
Wherein, the ratio of weight and number of each raw medicinal material can be Corm Eleocharitis 14 weight portions again in the described Chinese medicine preparation, Radix Rubiae 26 weight portions, Cornu Bubali 14 weight portions, the Radix Astragali 19 weight portions, Herba stellariae mediae 27 weight portions, Radix Codonopsis 23 weight portions, Herba Agrimoniae 26 weight portions, Herba Violae 22 weight portions, Radix Sophorae Tonkinensis 8 weight portions, Celastrus aceleatus Merr 21 weight portions, Radix Rehmanniae 24 weight portions, Rhizoma seu Herba Valerianae Tanguticae 19 weight portions, Rhizoma Imperatae 19 weight portions, Radix Ranunculi Ternati 14 weight portions, Placenta Hominis 23 weight portions, Cornu Saigae Tataricae 26 weight portions, Herba Artemisiae Annuae 28 weight portions, Receptaculum Nelumbinis 20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 21 weight portions, broken close 32 weight portions and Radix Glycyrrhizae 36 weight portions spent.
When the dosage form of described Chinese medicine preparation was syrup, its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, use the dissolve with ethanol with respect to 4～8 times determining alcohol 75%～85% of the mixture quality that obtains, reflux was extracted after 4～6 hours, filter, get the first filtrate; Filtering residue is used dissolve with ethanol with respect to 3～5 times determining alcohol 80%～90% of the mixture quality that obtains again, and reflux was extracted after 2～4 hours, and filtration gets the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol and to be concentrated into liquor strength be 0.6～0.8g crude drug/mL, utilize volume to be the macroporous adsorptive resins eluting of 5L, use first deionized water or the distilled water eluting of 5 times of resin column volumes, determining alcohol with 3 times of resin column volumes is 90%～95% ethanol elution again, collect ethanol elution, concentrating under reduced pressure is removed ethanol, and drying also is ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, the alcohol heating reflux that adds with respect to 3～6 times determining alcohol 85%～95% of the mixture quality that obtains extracts 2～4 times, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, relative density was 1.36～1.38 mastic when the extracting solution after the remove impurity was evaporated to 70 ℃ under 70 ℃～80 ℃ condition, and the mastic after concentrated is dried to powder with spray dryer;
The 3rd step, the surplus stock medical material is mixed in described ratio, add 6～10 times water with respect to the mixture quality that obtains, to soak 8-12 hour, heating decocted 4～8 hours, filtered; Filtering residue is used 3～6 times water dissolution with respect to the mixture quality that obtains again, and heating decocted 2～5 hours, filtered; Merge twice filtrate, concentrating under reduced pressure is removed moisture, and drying also is ground into powder;
The 4th step, the powder that the first step, second step and the 3rd step are obtained mixes, add the water dissolution with respect to 1.2～1.8 times of mixture qualities, regulate pH value to 7.0 with 40% sodium hydroxide solution, stir evenly, cold preservation is 60 hours～72 hours under 3 ℃～6 ℃ conditions, filter, filtrate adds the sucrose with respect to 0.5～0.8 times of the mixture quality that obtains, and stirs and dissolving, regulates pH value to 7.0, add water an amount of, stir, left standstill 12～18 hours, filter, fill, sterilization obtains syrup, and the crude drug concentration in the described syrup is 0.9g/mL～1.2g/mL.
When the dosage form of described Chinese medicine preparation was oral solutions, its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that 80%～90% methanol is as solvent with determining alcohol, adopt percolation with the speed of per minute 1～3ml percolation slowly, collect percolate, concentrating under reduced pressure is also dry, obtains to get dry extract, and is ground into powder; The quality of the methanol that adds is 4～6 times of first step mixture quality;
Second step is mixed in proportion the surplus stock medical material, is ground into powder;
The 3rd step in the powder that obtains to second step, added the distilled water of 5～8 times of the powder qualities that obtain with respect to second step, decocted 3～6 hours, and filtration obtains the first filtrate; With the distilled water of filtering residue adding with respect to 3～6 times of its quality, decocted 2～4 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3～3/4 volume, add determining alcohol and be 90～95% ethanol, make determining alcohol reach 70%～80%, left standstill 30～50 hours, filter, concentrating under reduced pressure, drying obtains and gets dry extract, and is ground into powder;
In the 4th step, the powder mixing with the first step and the acquisition of the 3rd step adds the distilled water with respect to 4～6 times of amounts of powder quality, 0.4～0.8 times sucrose, and 0.02～0.05 times sodium benzoate stirs, and boils, and cooling is filtered, packing, sterilizing namely obtains oral liquid.
Beneficial effect of the present invention: the present invention is pure Chinese medicinal preparation, without any side effects, the hemostasis and pain-relieving definite effect, rapid-action, therapeutic effect is good, taking convenience, long-term taking can not produce drug dependence, and raw material is easy to get, and drug cost is low, can help as early as possible the patient to break away from whole blood and thrombin, alleviate the financial burden of patient treatment.
The specific embodiment
The invention provides a kind of haemophiliachemophiliac Chinese medicine preparation for the treatment of, comprise following raw medicinal material: Corm Eleocharitis, Radix Rubiae, Cornu Bubali, the Radix Astragali, Herba stellariae mediae, Radix Codonopsis, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, Radix Rehmanniae, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae, Herba Artemisiae Annuae, Receptaculum Nelumbinis, Pseudobulbus Bletillae (Rhizoma Bletillae), broken close flower and Radix Glycyrrhizae.
Wherein, the pharmacology of each raw medicinal material is as follows in the Chinese medicine preparation:
Corm Eleocharitis: sweet in the mouth; Cold in nature.Return lung; The stomach warp.Clearing away heat and promoting production of body fluid; Reduce phlegm; Removing food stagnancy.Main epidemic febrile disease is thirsty; Laryngopharynx swelling and pain; Phlegm-heat cough; Conjunctival congestion; Quench one's thirst; Dysentery; Jaundice; Pyretic stranguria; Food stagnation; Wart.
Radix Rubiae: hardship, cold.Return Liver Channel.Removing heat from blood, hemostasis, blood stasis dispelling stimulates the menstrual flow.Be used for spitting blood epistaxis, metrorrhagia, traumatic hemorrhage, amenorrhea stasis blocking, arthralgia, tumbling and swelling.
Cornu Bubali: hardship, cold.GUIXIN, Liver Channel.Heat-clearing and toxic substances removing, removing heat from blood, arresting convulsion.Be used for the high heat of epidemic febrile disease, unconsciousness and delirium is sent out the speckle dermexanthesis, hematemesis and epistaxis, and infantile convulsion, demented.
The Radix Astragali: sweet, temperature.Return lung, spleen channel.Invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.Be used for the deficiency of vital energy weak, anorexia and loose stool, sinking of QI of middle-JIAO, the chronic diarrhea proctoptosis, the metrorrhagia of having blood in stool, the exterior deficiency spontaneous perspiration, deficiency of vital energy edema, carbuncle is difficult bursts, and burst for a long time and do not hold back, the blood deficiency dull yellowish colored skin, interior-heat is quenched one's thirst; Chronic nephritis proteinuria, diabetes.
Herba stellariae mediae: little hardship, sweet, sour; Cool in nature.Return liver; Large intestine channel.Heat-clearing and toxic substances removing; The removing heat from blood eliminating carbuncle; Promoting blood circulation and stopping pain; Stimulating milk secretion;Main dysentery; The intestinal carbuncle; Lung abscess; Acute mastitis; Furuncle swelling toxin; Swelling and pain of hemorrhoid; Hemorrhage; Traumatic pain; The stasis of blood stagnated and suffered from abdominal pain puerperal; Agalactia.
Radix Codonopsis: sweet, flat.Return spleen, lung meridian.Invigorating the spleen and replenishing QI, the spleen invigorating lung benefiting.Be used for deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, the dyspnea due to deficiency cough, interior-heat is quenched one's thirst.
Herba Agrimoniae: bitter in the mouth, puckery, property is flat.Return lung; Liver; Spleen channel.Astringing to arrest bleeding, dysentery relieving, parasite killing.Main spitting of blood; Spit blood; Hematuria; Have blood in stool; Dysentery; Bleeding not during menses; Impairment caused by overstrain is taken off power; Carbuncle; Traumatic injury; Wound hemorrhage.
Herba Violae: bitter, hot, cold.GUIXIN, Liver Channel.Heat-clearing and toxic substances removing, the removing heat from blood detumescence.Be used for furuncle swelling toxin, carbuncle carbuncle on the back, erysipelas, venom.
Radix Sophorae Tonkinensis: hardship, cold; Poisonous.Attach to the lung and stomach meridians.Heat-clearing and toxic substances removing, the detumescence sore-throat relieving.Be used for fire-toxin and pent up laryngopharynx swelling and pain, gingivitis.
Celastrus aceleatus Merr: hardship; Hot; Cool.Return liver; Gallbladder; Kidney channel.Heat-clearing and toxic substances removing; Expelling wind and removing dampness.The main air pain of dampness syndrome; Gout; Nephritis; Cholecystitis; Leukemia.
Radix Rehmanniae: sweet, cold.GUIXIN, liver, kidney channel.Clearing away heat and cooling blood, yin nourishing is promoted the production of body fluid.Be used for calentura crimson tongue excessive thirst, yin asthenia generating intrinsic heat, the hectic fever due to YIN-deficiency consumptive fever, interior-heat is quenched one's thirst, and spits blood, and epistaxis is sent out the speckle dermexanthesis.
Rhizoma seu Herba Valerianae Tanguticae: sweet in the mouth; Little suffering; Property is put down.Cough-relieving; Hemostasis; Dissipating blood stasis; Pain relieving.Main cough; Hemoptysis; Spit blood; Epistaxis; Metrostaxis; Rheumatic arthralgia; Fracture.
Rhizoma Imperatae: sweet, cold.Return lung, stomach, urinary bladder channel.Cooling blood for hemostasis, clearing away heat and promoting diuresis.Be used for heat in blood and spit blood epistaxis, hematuria, calentura excessive thirst, jaundice, edema, the puckery pain of pyretic stranguria; Acute nephritis edema.
Radix Ranunculi Ternati: sweet, hot, temperature.Return liver, lung meridian.Eliminating stagnation, detumescence., tuberculous lymphadenitis routed for scrofula.
Placenta Hominis: sweet, one-tenth, temperature.GUIXIN, lung, kidney channel.The kidney warming is mended essence, benefiting QI and nourishing blood.Be used for the asthenia win thin, hectic fever due to YIN-deficiency night sweat, cough and asthma, lack of appetite is breathed hard, impotence and seminal emission, infertile hypogalactia.
Cornu Saigae Tataricae: become, cold.Return liver, heart channel.Suppressing the hyperactive liver to relieve the wind syndrome, liver heat removing and eyesight improving, loose blood detoxifcation.Be used for high hot infantile convulsion, the coma convulsion is fainted, and eclamposia is twitched, and epilepsy is gone mad, and has a headache dizzy conjunctival congestion cataracta, maculae caused by violent heat pathogen, carbuncle sore tumefacting virus.
Herba Artemisiae Annuae: bitter, hot, cold.Return liver, gallbladder meridian.Clearing away summer-heat is except steaming preventing the attack (or recurrence) of malaria.Be used for the pathogenic summer-heat heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to YIN-deficiency consumptive fever, malaria cold and heat, jaundice due to damp-heat.
Receptaculum Nelumbinis: bitter, puckery, temperature.Return Liver Channel.Removing stasis to stop bleeding.Be used for metrorrhagia, hematuria, bleeding hemorrhoids, postpartum stagnation, lochiorrhea.
Pseudobulbus Bletillae (Rhizoma Bletillae): hardship; Sweet; Puckery; Cold.Return lung; The stomach warp.Astringing to arrest bleeding; Detumescence and promoting granulation.Main spitting of blood; Spit blood; Epistaxis; Have blood in stool; Traumatic hemorrhage; The carbuncle pyogenic infections from tumour or sore; Scald and burn; Rhagadia manus et pedis; Anal fissure
Broken close flower: bitter in the mouth; Cool in nature.The detoxifcation dysentery relieving; The synthetism hemostasis.Main toxic-heat and blood stasis; Traumatic injury is turned round and is improperly familiar with; Fracture; Traumatic hemorrhage.
Radix Glycyrrhizae: sweet, flat.GUIXIN, lung, spleen, stomach warp.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.Be used for weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, gastral cavity abdomen, the anxious pain of extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strong.
Wherein, the ratio of weight and number of each raw medicinal material can be Corm Eleocharitis 10～15 weight portions in the described Chinese medicine preparation, Radix Rubiae 20～40 weight portions, Cornu Bubali 10～25 weight portions, the Radix Astragali 15～30 weight portions, Herba stellariae mediae 20～40 weight portions, Radix Codonopsis 20～30 weight portions, Herba Agrimoniae 15～30 weight portions, Herba Violae 20～35 weight portions, Radix Sophorae Tonkinensis 6～12 weight portions, Celastrus aceleatus Merr 15～25 weight portions, Radix Rehmanniae 20～30 weight portions, Rhizoma seu Herba Valerianae Tanguticae 10～20 weight portions, Rhizoma Imperatae 15～20 weight portions, Radix Ranunculi Ternati 10～20 weight portions, Placenta Hominis 20～30 weight portions, Cornu Saigae Tataricae 20～40 weight portions, Herba Artemisiae Annuae 10～30 weight portions, Receptaculum Nelumbinis 10～20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 20～30 weight portions, broken close 20～40 weight portions and Radix Glycyrrhizae 20～40 weight portions spent.
Wherein, the ratio of weight and number of each raw medicinal material can also be Corm Eleocharitis 12～15 weight portions in the described Chinese medicine preparation, Radix Rubiae 20～30 weight portions, Cornu Bubali 10～20 weight portions, the Radix Astragali 15～20 weight portions, Herba stellariae mediae 20～30 weight portions, Radix Codonopsis 20～25 weight portions, Herba Agrimoniae 20～30 weight portions, Herba Violae 20～30 weight portions, Radix Sophorae Tonkinensis 6～10 weight portions, Celastrus aceleatus Merr 20～25 weight portions, Radix Rehmanniae 20～25 weight portions, Rhizoma seu Herba Valerianae Tanguticae 16～20 weight portions, Rhizoma Imperatae 18～20 weight portions, Radix Ranunculi Ternati 10～16 weight portions, Placenta Hominis 20～26 weight portions, Cornu Saigae Tataricae 20～30 weight portions, Herba Artemisiae Annuae 20～30 weight portions, Receptaculum Nelumbinis 15～20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 20～25 weight portions, broken close 25～35 weight portions and Radix Glycyrrhizae 30～40 weight portions spent.
Wherein, the ratio of weight and number of each raw medicinal material can be Corm Eleocharitis 14 weight portions again in the described Chinese medicine preparation, Radix Rubiae 26 weight portions, Cornu Bubali 14 weight portions, the Radix Astragali 19 weight portions, Herba stellariae mediae 27 weight portions, Radix Codonopsis 23 weight portions, Herba Agrimoniae 26 weight portions, Herba Violae 22 weight portions, Radix Sophorae Tonkinensis 8 weight portions, Celastrus aceleatus Merr 21 weight portions, Radix Rehmanniae 24 weight portions, Rhizoma seu Herba Valerianae Tanguticae 19 weight portions, Rhizoma Imperatae 19 weight portions, Radix Ranunculi Ternati 14 weight portions, Placenta Hominis 23 weight portions, Cornu Saigae Tataricae 26 weight portions, Herba Artemisiae Annuae 28 weight portions, Receptaculum Nelumbinis 20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 21 weight portions, broken close 32 weight portions and Radix Glycyrrhizae 36 weight portions spent.
When the dosage form of described Chinese medicine preparation was syrup, its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, use the dissolve with ethanol with respect to 4～8 times determining alcohol 75%～85% of the mixture quality that obtains, reflux was extracted after 4～6 hours, filter, get the first filtrate; Filtering residue is used dissolve with ethanol with respect to 3～5 times determining alcohol 80%～90% of the mixture quality that obtains again, and reflux was extracted after 2～4 hours, and filtration gets the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol and to be concentrated into liquor strength be 0.6～0.8g crude drug/mL, utilize volume to be the macroporous adsorptive resins eluting of 5L, use first deionized water or the distilled water eluting of 5 times of resin column volumes, determining alcohol with 3 times of resin column volumes is 90%～95% ethanol elution again, collect ethanol elution, concentrating under reduced pressure is removed ethanol, and drying also is ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, the alcohol heating reflux that adds with respect to 3～6 times determining alcohol 85%～95% of the mixture quality that obtains extracts 2～4 times, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, relative density was 1.36～1.38 mastic when the extracting solution after the remove impurity was evaporated to 70 ℃ under 70 ℃～80 ℃ condition, and the mastic after concentrated is dried to powder with spray dryer;
The 3rd step, the surplus stock medical material is mixed in described ratio, add 6～10 times water with respect to the mixture quality that obtains, to soak 8-12 hour, heating decocted 4～8 hours, filtered; Filtering residue is used 3～6 times water dissolution with respect to the mixture quality that obtains again, and heating decocted 2～5 hours, filtered; Merge twice filtrate, concentrating under reduced pressure is removed moisture, and drying also is ground into powder;
The 4th step, the powder that the first step, second step and the 3rd step are obtained mixes, add the water dissolution with respect to 1.2～1.8 times of mixture qualities, regulate pH value to 7.0 with 40% sodium hydroxide solution, stir evenly, cold preservation is 60 hours～72 hours under 3 ℃～6 ℃ conditions, filter, filtrate adds the sucrose with respect to 0.5～0.8 times of the mixture quality that obtains, and stirs and dissolving, regulates pH value to 7.0, add water an amount of, stir, left standstill 12～18 hours, filter, fill, sterilization obtains syrup, and the crude drug concentration in the described syrup is 0.9g/mL～1.2g/mL.
When the dosage form of described Chinese medicine preparation was oral solutions, its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that 80%～90% methanol is as solvent with determining alcohol, adopt percolation with the speed of per minute 1～3ml percolation slowly, collect percolate, concentrating under reduced pressure is also dry, obtains to get dry extract, and is ground into powder; The quality of the methanol that adds is 4～6 times of first step mixture quality;
Second step is mixed in proportion the surplus stock medical material, is ground into powder;
The 3rd step in the powder that obtains to second step, added the distilled water of 5～8 times of the powder qualities that obtain with respect to second step, decocted 3～6 hours, and filtration obtains the first filtrate; With the distilled water of filtering residue adding with respect to 3～6 times of its quality, decocted 2～4 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3～3/4 volume, add determining alcohol and be 90～95% ethanol, make determining alcohol reach 70%～80%, left standstill 30～50 hours, filter, concentrating under reduced pressure, drying obtains and gets dry extract, and is ground into powder;
In the 4th step, the powder mixing with the first step and the acquisition of the 3rd step adds the distilled water with respect to 4～6 times of amounts of powder quality, 0.4～0.8 times sucrose, and 0.02～0.05 times sodium benzoate stirs, and boils, and cooling is filtered, packing, sterilizing namely obtains oral liquid.
Below adopt embodiment to describe embodiments of the present invention in detail, how the application technology means solve technical problem to the present invention whereby, and the implementation procedure of reaching technique effect can fully understand and implements according to this.
Embodiment 1 syrup of the present invention
Get Corm Eleocharitis 140g, Radix Rubiae 260g, Cornu Bubali 140g, Radix Astragali 190g, Herba stellariae mediae 270g, Radix Codonopsis 230g, Herba Agrimoniae 260g, Herba Violae 220g, Radix Sophorae Tonkinensis 80g, Celastrus aceleatus Merr 210g, Radix Rehmanniae 240g, Rhizoma seu Herba Valerianae Tanguticae 190g, Rhizoma Imperatae 190g, Radix Ranunculi Ternati 140g, Placenta Hominis 230g, Cornu Saigae Tataricae 260g, Herba Artemisiae Annuae 280g, Receptaculum Nelumbinis 200g, Pseudobulbus Bletillae (Rhizoma Bletillae) 210g, broken close colored 320g and Radix Glycyrrhizae 360g;
Its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in proportion, use the dissolve with ethanol with respect to 6 times determining alcohol 80% of the mixture quality that obtains, reflux was extracted after 5 hours, filter, get the first filtrate; Filtering residue is used dissolve with ethanol with respect to 3 times determining alcohol 90% of the mixture quality that obtains again, and reflux was extracted after 3 hours, and filtration gets the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol and to be concentrated into liquor strength be 0.7g crude drug/mL, utilize volume to be the macroporous adsorptive resins eluting of 5L, use first deionized water or the distilled water eluting of 5 times of resin column volumes, determining alcohol with 3 times of resin column volumes is 95% ethanol elution again, collect ethanol elution, concentrating under reduced pressure is removed ethanol, and drying also is ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in proportion, the alcohol heating reflux that adds with respect to 4 times determining alcohol 90% of the mixture quality that obtains extracts 3 times, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, relative density was 1.37 mastic when the extracting solution after the remove impurity was evaporated to 70 ℃ under 80 ℃ condition, and the mastic after concentrated is dried to powder with spray dryer;
The 3rd step was mixed in proportion the surplus stock medical material, added 8 times water with respect to the mixture quality that obtains, and soaked 10 hours, and heating decocted 7 hours, filtered; Filtering residue is used 5 times water dissolution with respect to the mixture quality that obtains again, and heating decocted 3 hours, filtered; Merge twice filtrate, concentrating under reduced pressure is removed moisture, and drying also is ground into powder;
In the 4th step, the powder that the first step, second step and the 3rd step are obtained mixes, and adds the water dissolution with respect to 1.6 times of mixture qualities, regulate pH value to 7.0 with 40% sodium hydroxide solution, stir evenly, cold preservation is 65 hours under 4 ℃ of conditions, filter, filtrate adds the sucrose with respect to 0.7 times of the mixture quality that obtains, and stirs and dissolving, regulate pH value to 7.0, add water an amount of, stir, left standstill 16 hours, and filtered fill, sterilization obtains syrup, and the crude drug concentration in the described syrup is 1.0g/mL.
Embodiment 2 oral solutionses of the present invention
Get Corm Eleocharitis 120g, Radix Rubiae 240g, Cornu Bubali 170g, Radix Astragali 130g, Herba stellariae mediae 290g, Radix Codonopsis 210g, Herba Agrimoniae 220g, Herba Violae 270g, Radix Sophorae Tonkinensis 90g, Celastrus aceleatus Merr 230g, Radix Rehmanniae 210g, Rhizoma seu Herba Valerianae Tanguticae 160g, Rhizoma Imperatae 200g, Radix Ranunculi Ternati 120g, Placenta Hominis 250g, Cornu Saigae Tataricae 210g, Herba Artemisiae Annuae 220g, Receptaculum Nelumbinis 160g, Pseudobulbus Bletillae (Rhizoma Bletillae) 240g, broken close colored 270g and Radix Glycyrrhizae 310g;
Its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that 85% methanol is as solvent with determining alcohol, adopt percolation with the speed of per minute 2ml percolation slowly, collect percolate, concentrating under reduced pressure is also dry, obtains to get dry extract, and is ground into powder; The quality of the methanol that adds is 5 times of first step mixture quality;
Second step is mixed in proportion the surplus stock medical material, is ground into powder;
The 3rd step in the powder that obtains to second step, added the distilled water of 6 times of the powder qualities that obtain with respect to second step, decocted 4 hours, and filtration obtains the first filtrate; With the distilled water of filtering residue adding with respect to 4 times of its quality, decocted 3 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3 volume, add determining alcohol and be 90% ethanol, make determining alcohol reach 75%, left standstill 35 hours, filter, concentrating under reduced pressure, drying obtains and gets dry extract, and is ground into powder;
In the 4th step, the powder mixing with the first step and the acquisition of the 3rd step adds the distilled water with respect to 5 times of amounts of powder quality, 0.6 times sucrose, and 0.03 times sodium benzoate stirs, and boils, and cooling is filtered, packing, sterilizing namely obtains oral liquid.
Poison is given birth to test:
Use 80 of NIH mices, the SPF level, male and female half and half, body weight 14～22g carries out acute toxicity testing.Mice is divided into two groups at random, be matched group and administration group, fasting is 12 hours before the experiment, with the Chinese materia medica syrup of embodiments of the invention 1 preparation, gavage, the gavage volume is that 40m1/kg (is that single-dose dosage is 40g crude drug/kg), matched group gives the equivalent normal saline, administration in 1 day 2 times, delivery time 6 hours, Continuous Observation is 14 days after the administration, and toxic reaction and the death toll of record mice.Experimental result shows: with matched group relatively, mice has no notable difference after the administration, experiment Continuous Observation 14 days, the mice overall health of patients, ingest, drinking-water, body weight gain is all normal.Mice oral administration gavage syrup LD of the present invention
50＞40g crude drug/kg, every day, maximum dosage-feeding was 80g crude drug/kg/ day.Chinese medicine clinical application amount of the present invention becomes body weight for humans in 60kg in 10g crude drug/day/people, and average dosage is 0.167g crude drug/kg/ day.By weighing machine: the dosis tolerata of mice (average weight is take 20g) oral administration gavage Chinese medicine of the present invention is 479 times of quantity.Therefore Chinese medicine acute toxicity of the present invention is low, clinical drug safety.
Clinical data:
Case is selected: get the 69 routine sufferers that in August, 2007～2012 derive from the patient of the court year November, be the male.Hemophilia A 62 examples; Hemophilia B 7 examples; The course of disease 4～22 years old has obvious hematostaxis history, and how medical because of oral cavity, muscle or arthrorrhagia, 46 examples have family history.
Diagnostic criteria: the diagnostic criteria of hemophilia A: 1. clinical manifestation: the male patient has or without family history.Accordance joins stealthy genetic development.The female homozygote type can occur, and seldom sees; Joint, muscle, deep tissue are hemorrhage, can be spontaneous.The joint Repeated Hemorrhage causes joint deformity, and the deep tissue Repeated Hemorrhage causes false tumor (hematocele).2. lab testing: clotting time (test tube method) severe prolongs, and moderate can be normal, and is slight and Subclinical is normal.The activated partial clotting time, severe obviously prolongs, and can be corrected by fresh normal blood plasma and adsorbed plasma, slightly slightly prolongation or normal.Platelet count, bleeding time and blood clot retraction are normal, prothrombin time is normal.FVIII procoagulant activity (FVIII:C) reduces.3. order of severity typing: heavy type: FVIII:C＜2%, muscle, joint and deep tissue are hemorrhage, joint deformity, false tumor.Medium-sized: FVIII:C2～5%, can there be joint, muscle and deep tissue hemorrhage, joint deformity, but lighter.Light-duty: FVIII:C6～25%, joint and hemorrhage of muscle are seldom, and is lopsided without the joint.Subclinical: FVIII:C26～45% is only at severe trauma or postoperative hemorrhage.The diagnostic criteria of hemophilia B: 1. clinical manifestation: same hemophilia A.2. lab testing: APTT prolongs, and can be corrected by normal serum, but correct unlike normal sorption blood plasma, light-duty can be normal.Plasma F IX:C measures and reduces or shortage.The same hemophilia A of order of severity typing.
Exclusion standard: von Willebrand's disease patient; And the coagulation factor deficiency that causes because of other reasons; The deficiency of coagulation factors that poisoning causes such as mouse killing medicine is hemorrhage, and it is hemorrhage that anticoagulant such as warfarin etc. causes.
Therapeutic Method: take the syrup of the embodiment of the invention 1 preparation, each 5ml, every day 2 times, continuous 7 days is 1 course for the treatment of.
The standard of curative effect evaluation: clinical cure: bleeding disappears, and hematoma dissipates, and it is normal that the joint walking function recovers, and it is normal that the indices such as laboratory examination APTT recover, and FVIII:C or FIX:C return at least 50%-60%.Produce effects: bleeding obviously improves, and hematoma dissipates substantially, and the joint walking function improves, and the indices such as laboratory examination APTT approach normal, and FVIII:C or FIX:C return to 30%-40%.Invalid: indices is without improving or improving less.
Therapeutic outcome: the leading indicator average detection the results are shown in Table 1 before and after the treatment.
Take Chinese medicine preparation of the present invention, among the 69 routine patients, 66 examples (95.65%) patient can produce obvious hemostasis and pain-relieving effect in 24 hours;
After taking 1 course for the treatment of, the frequency of hematostaxis etc. obviously reduce, and the dermal ecchymosis disappears, and muscle power obviously strengthens;
After taking 2 courses for the treatment of, self coagulation factor activity of patient is improved significantly, and wherein 36 examples (52.17%) patient can no longer need to input whole blood and thrombin, clinical cure 33 examples (47.83%), produce effects 32 examples (46.38%), invalid 4 examples (5.8%);
Take medicine after 3 courses for the treatment of, self coagulation factor activity of 80% above patient returns to 45%-60%, wherein 59 examples (85.51%) patient can no longer need to input whole blood and thrombin, clinical cure 54 examples (78.26%), produce effects 14 examples (20.29%), invalid 1 example (1.45%);
Take 4 courses for the treatment of, self coagulation factor activity of 85% above patient returns at least 50%-60%, it is hemorrhage that the positions such as skin, gingiva, joint no longer appear in the patient, the lopsided joint function disturbance of patient's enlargement recovers gradually, degree of bending and stretching obviously strengthens, and the periarticular muscle of atrophy obviously recovers, clinical cure 65 examples (94.20%), produce effects 4 examples (5.8%), invalid 0 example (0%).
Leading indicator average detection result before and after table 1 treatment
As shown in Table 1, compare after the treatment with before the treatment, PT, APTT, TT and STGT shorten (P＜0.05), FVIII:C value and FIX:C and raise (P＜0.05), namely after Chinese medicine preparation of the present invention is treated, can make indices return to normal range or near normal range.
Model case
Case 1: Lee, man, 16 years old, suffer from since the childhood hemophilia, right knee joint swells and ache and repeatedly shows effect 4 years, and flexion deformity was squatted down difficult 2 years, have a medical check-up: right arthroncus of knee joints, violent tenderness, joint week footpath 38cm (left side 35cm), friction sense (++), stretch 180 °, bent 65 °, FVIII:C2.67%, APTT83.25s.Take the syrup of the embodiment of the invention 1 preparation, each 5m1, every day 2 times, continuous 7 days is 1 course for the treatment of.Take 3 courses for the treatment of, coagulation factor activity returns to 51.38%, and the lopsided joint function disturbance of patient's enlargement obviously recovers, and degree of bending and stretching obviously strengthens, arthroncus and this disappearance of tenderness sensillary base.Continue to take medicine 1 course for the treatment of, symptom disappears substantially, can be freely movable.Leave hospital, continue to take 1 course for the treatment of and consolidated.Followed up a case by regular visits to 1 year, stable disease is without recurrence.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment does, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (8)
1. treat haemophiliachemophiliac Chinese medicine preparation for one kind, it is characterized in that, comprise following raw medicinal material: Corm Eleocharitis, Radix Rubiae, Cornu Bubali, the Radix Astragali, Herba stellariae mediae, Radix Codonopsis, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, Radix Rehmanniae, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae, Herba Artemisiae Annuae, Receptaculum Nelumbinis, Pseudobulbus Bletillae (Rhizoma Bletillae), broken close flower and Radix Glycyrrhizae.
2. Chinese medicine preparation as claimed in claim 1, it is characterized in that, the ratio of weight and number of each raw medicinal material is in the described Chinese medicine preparation: Corm Eleocharitis 10～15 weight portions, Radix Rubiae 20～40 weight portions, Cornu Bubali 10～25 weight portions, the Radix Astragali 15～30 weight portions, Herba stellariae mediae 20～40 weight portions, Radix Codonopsis 20～30 weight portions, Herba Agrimoniae 15～30 weight portions, Herba Violae 20～35 weight portions, Radix Sophorae Tonkinensis 6～12 weight portions, Celastrus aceleatus Merr 15～25 weight portions, Radix Rehmanniae 20～30 weight portions, Rhizoma seu Herba Valerianae Tanguticae 10～20 weight portions, Rhizoma Imperatae 15～20 weight portions, Radix Ranunculi Ternati 10～20 weight portions, Placenta Hominis 20～30 weight portions, Cornu Saigae Tataricae 20～40 weight portions, Herba Artemisiae Annuae 10～30 weight portions, Receptaculum Nelumbinis 10～20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 20～30 weight portions, broken close 20～40 weight portions and Radix Glycyrrhizae 20～40 weight portions spent.
3. Chinese medicine preparation as claimed in claim 1 or 2, it is characterized in that, the ratio of weight and number of each raw medicinal material is in the described Chinese medicine preparation: Corm Eleocharitis 12～15 weight portions, Radix Rubiae 20～30 weight portions, Cornu Bubali 10～20 weight portions, the Radix Astragali 15～20 weight portions, Herba stellariae mediae 20～30 weight portions, Radix Codonopsis 20～25 weight portions, Herba Agrimoniae 20～30 weight portions, Herba Violae 20～30 weight portions, Radix Sophorae Tonkinensis 6～10 weight portions, Celastrus aceleatus Merr 20～25 weight portions, Radix Rehmanniae 20～25 weight portions, Rhizoma seu Herba Valerianae Tanguticae 16～20 weight portions, Rhizoma Imperatae 18～20 weight portions, Radix Ranunculi Ternati 10～16 weight portions, Placenta Hominis 20～26 weight portions, Cornu Saigae Tataricae 20～30 weight portions, Herba Artemisiae Annuae 20～30 weight portions, Receptaculum Nelumbinis 15～20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 20～25 weight portions, broken close 25～35 weight portions and Radix Glycyrrhizae 30～40 weight portions spent.
4. such as the described Chinese medicine preparation of claims 1 to 3, it is characterized in that, the ratio of weight and number of each raw medicinal material is in the described Chinese medicine preparation: Corm Eleocharitis 14 weight portions, Radix Rubiae 26 weight portions, Cornu Bubali 14 weight portions, the Radix Astragali 19 weight portions, Herba stellariae mediae 27 weight portions, Radix Codonopsis 23 weight portions, Herba Agrimoniae 26 weight portions, Herba Violae 22 weight portions, Radix Sophorae Tonkinensis 8 weight portions, Celastrus aceleatus Merr 21 weight portions, Radix Rehmanniae 24 weight portions, Rhizoma seu Herba Valerianae Tanguticae 19 weight portions, Rhizoma Imperatae 19 weight portions, Radix Ranunculi Ternati 14 weight portions, Placenta Hominis 23 weight portions, Cornu Saigae Tataricae 26 weight portions, Herba Artemisiae Annuae 28 weight portions, Receptaculum Nelumbinis 20 weight portions, Pseudobulbus Bletillae (Rhizoma Bletillae) 21 weight portions, broken close 32 weight portions and Radix Glycyrrhizae 36 weight portions spent.
5. such as the described Chinese medicine preparation of claim 1 to 4, it is characterized in that, when the dosage form of described Chinese medicine preparation was syrup, its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, use the dissolve with ethanol with respect to 4～8 times determining alcohol 75%～85% of the mixture quality that obtains, reflux was extracted after 4～6 hours, filter, get the first filtrate; Filtering residue is used dissolve with ethanol with respect to 3～5 times determining alcohol 80%～90% of the mixture quality that obtains again, and reflux was extracted after 2～4 hours, and filtration gets the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol and to be concentrated into liquor strength be 0.6～0.8g crude drug/mL, utilize volume to be the macroporous adsorptive resins eluting of 5L, use first deionized water or the distilled water eluting of 5 times of resin column volumes, determining alcohol with 3 times of resin column volumes is 90%～95% ethanol elution again, collect ethanol elution, concentrating under reduced pressure is removed ethanol, and drying also is ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, the alcohol heating reflux that adds with respect to 3～6 times determining alcohol 85%～95% of the mixture quality that obtains extracts 2～4 times, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, relative density was 1.36～1.38 mastic when the extracting solution after the remove impurity was evaporated to 70 ℃ under 70 ℃～80 ℃ condition, and the mastic after concentrated is dried to powder with spray dryer;
The 3rd step, the surplus stock medical material is mixed in described ratio, add 6～10 times water with respect to the mixture quality that obtains, to soak 8-12 hour, heating decocted 4～8 hours, filtered; Filtering residue is used 3～6 times water dissolution with respect to the mixture quality that obtains again, and heating decocted 2～5 hours, filtered; Merge twice filtrate, concentrating under reduced pressure is removed moisture, and drying also is ground into powder;
The 4th step, the powder that the first step, second step and the 3rd step are obtained mixes, add the water dissolution with respect to 1.2～1.8 times of mixture qualities, regulate pH value to 7.0 with 40% sodium hydroxide solution, stir evenly, cold preservation is 60 hours～72 hours under 3 ℃～6 ℃ conditions, filter, filtrate adds the sucrose with respect to 0.5～0.8 times of the mixture quality that obtains, and stirs and dissolving, regulates pH value to 7.0, add water an amount of, stir, left standstill 12～18 hours, filter, fill, sterilization obtains syrup, and the crude drug concentration in the described syrup is 0.9g/mL～1.2g/mL.
6. such as the described Chinese medicine preparation of claim 1 to 4, it is characterized in that, when the dosage form of described Chinese medicine preparation was oral solutions, its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that 80%～90% methanol is as solvent with determining alcohol, adopt percolation with the speed of per minute 1～3ml percolation slowly, collect percolate, concentrating under reduced pressure is also dry, obtains to get dry extract, and is ground into powder; The quality of the methanol that adds is 4～6 times of first step mixture quality;
Second step is mixed in proportion the surplus stock medical material, is ground into powder;
The 3rd step in the powder that obtains to second step, added the distilled water of 5～8 times of the powder qualities that obtain with respect to second step, decocted 3～6 hours, and filtration obtains the first filtrate; With the distilled water of filtering residue adding with respect to 3～6 times of its quality, decocted 2～4 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3～3/4 volume, add determining alcohol and be 90～95% ethanol, make determining alcohol reach 70%～80%, left standstill 30～50 hours, filter, concentrating under reduced pressure, drying obtains and gets dry extract, and is ground into powder;
In the 4th step, the powder mixing with the first step and the acquisition of the 3rd step adds the distilled water with respect to 4～6 times of amounts of powder quality, 0.4～0.8 times sucrose, and 0.02～0.05 times sodium benzoate stirs, and boils, and cooling is filtered, packing, sterilizing namely obtains oral liquid.
7. the preparation method of the described Chinese medicine preparation of claim 1 to 4 is characterized in that, the dosage form of described Chinese medicine preparation is syrup, and its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, use the dissolve with ethanol with respect to 4～8 times determining alcohol 75%～85% of the mixture quality that obtains, reflux was extracted after 4～6 hours, filter, get the first filtrate; Filtering residue is used dissolve with ethanol with respect to 3～5 times determining alcohol 80%～90% of the mixture quality that obtains again, and reflux was extracted after 2～4 hours, and filtration gets the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol and to be concentrated into liquor strength be 0.6～0.8g crude drug/mL, utilize volume to be the macroporous adsorptive resins eluting of 5L, use first deionized water or the distilled water eluting of 5 times of resin column volumes, determining alcohol with 3 times of resin column volumes is 90%～95% ethanol elution again, collect ethanol elution, concentrating under reduced pressure is removed ethanol, and drying also is ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, the alcohol heating reflux that adds with respect to 3～6 times determining alcohol 85%～95% of the mixture quality that obtains extracts 2～4 times, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, relative density was 1.36～1.38 mastic when the extracting solution after the remove impurity was evaporated to 70 ℃ under 70 ℃～80 ℃ condition, and the mastic after concentrated is dried to powder with spray dryer;
The 3rd step, the surplus stock medical material is mixed in described ratio, add 6～10 times water with respect to the mixture quality that obtains, to soak 8-12 hour, heating decocted 4～8 hours, filtered; Filtering residue is used 3～6 times water dissolution with respect to the mixture quality that obtains again, and heating decocted 2～5 hours, filtered; Merge twice filtrate, concentrating under reduced pressure is removed moisture, and drying also is ground into powder;
The 4th step, the powder that the first step, second step and the 3rd step are obtained mixes, add the water dissolution with respect to 1.2～1.8 times of mixture qualities, regulate pH value to 7.0 with 40% sodium hydroxide solution, stir evenly, cold preservation is 60 hours～72 hours under 3 ℃～6 ℃ conditions, filter, filtrate adds the sucrose with respect to 0.5～0.8 times of the mixture quality that obtains, and stirs and dissolving, regulates pH value to 7.0, add water an amount of, stir, left standstill 12～18 hours, filter, fill, sterilization obtains syrup, and the crude drug concentration in the described syrup is 0.9g/mL～1.2g/mL.
8. the preparation method of the described Chinese medicine preparation of claim 1 to 4 is characterized in that, the dosage form of described Chinese medicine preparation is oral solutions, and its preparation method may further comprise the steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that 80%～90% methanol is as solvent with determining alcohol, adopt percolation with the speed of per minute 1～3ml percolation slowly, collect percolate, concentrating under reduced pressure is also dry, obtains to get dry extract, and is ground into powder; The quality of the methanol that adds is 4～6 times of first step mixture quality;
Second step is mixed in proportion the surplus stock medical material, is ground into powder;
The 3rd step in the powder that obtains to second step, added the distilled water of 5～8 times of the powder qualities that obtain with respect to second step, decocted 3～6 hours, and filtration obtains the first filtrate; With the distilled water of filtering residue adding with respect to 3～6 times of its quality, decocted 2～4 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3～3/4 volume, add determining alcohol and be 90～95% ethanol, make determining alcohol reach 70%～80%, left standstill 30～50 hours, filter, concentrating under reduced pressure, drying obtains and gets dry extract, and is ground into powder;
In the 4th step, the powder mixing with the first step and the acquisition of the 3rd step adds the distilled water with respect to 4～6 times of amounts of powder quality, 0.4～0.8 times sucrose, and 0.02～0.05 times sodium benzoate stirs, and boils, and cooling is filtered, packing, sterilizing namely obtains oral liquid.
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| CN104306783A (en) * | 2014-11-06 | 2015-01-28 | 徐彧 | Traditional Chinese medicine for treating hemophilia and preparation method thereof |
| CN105055927A (en) * | 2015-07-21 | 2015-11-18 | 陶艳玲 | Traditional Chinese medicine for treating infantile hemophilia a and preparation method of traditional Chinese medicine |
| CN105327134A (en) * | 2015-12-11 | 2016-02-17 | 孙殿亮 | Traditional Chinese medicine for treating factor VIII deficiency and preparing method thereof |
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| CN103599335A (en) * | 2013-12-05 | 2014-02-26 | 李凯 | Traditional Chinese medicine for treatment of chronic colitis |
| CN103599335B (en) * | 2013-12-05 | 2015-07-08 | 解安庆 | Traditional Chinese medicine for treatment of chronic colitis |
| CN104306783A (en) * | 2014-11-06 | 2015-01-28 | 徐彧 | Traditional Chinese medicine for treating hemophilia and preparation method thereof |
| CN105055927A (en) * | 2015-07-21 | 2015-11-18 | 陶艳玲 | Traditional Chinese medicine for treating infantile hemophilia a and preparation method of traditional Chinese medicine |
| CN105327134A (en) * | 2015-12-11 | 2016-02-17 | 孙殿亮 | Traditional Chinese medicine for treating factor VIII deficiency and preparing method thereof |
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