CN103301338B - Traditional Chinese medicine preparation for treating hemophilia and preparation method thereof - Google Patents
Traditional Chinese medicine preparation for treating hemophilia and preparation method thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine preparation for treating hemophilia and a preparation method thereof. The traditional Chinese medicine is prepared from the following crude drugs of water chestnut, radix rubiae, buffalo horn, astragalus membranaceus, chickweed, codonopsis pilosula, hairyvein agrimony, herba violae, subprostrate sophora, celastrus aculeatus, dried rehamnnia root, fragrant hair grass, lalang grass rhizome, radix ranunculi ternate, placenta hominis, antelope horn, sweet wormwood, receptaculum nelumbinis, rhizoma bletillae, broken dense flowers and liquorice. The traditional Chinese medicine preparation has the beneficial effects that the traditional Chinese medicine preparation is a pure traditional Chinese medicine preparation, free of toxic and side effects, accurate in effect of bleeding pain, fast to become effective, good in treatment effect, convenient to take, available in materials, and low in drug cost, does not generate the drug dependence after being taken for a long period of time, and can help a sufferer get rid of whole blood and blood coagulation factors; the economical burden on treatment of the sufferer is reduced.
Description
Technical field
The present invention relates to pharmaceutical technology, particularly relate to one and treat haemophiliachemophiliac Chinese medicine preparation and preparation method.
Background technology
Hemophilia is the hemorrhage that one group of hereditary coagulation factors deficiency causes.Thrombin be in human body one group there is the biological activity protein causing blood coagulation, have hemostatic function, main thrombin has 13 kinds, and conventional Roman number is expressed as: I, II....XIII (i.e. thrombin one, two ... to 13).If lack a certain thrombin in blood, blood is just not easy to solidify, thus causing bleeding property disease.In China, haemophiliachemophiliac social man group sickness rate is 5 ~ 10,/10 ten thousand, baby's incidence rate about 1/5000.Often there is coagulation disorders in classical haemophilia patient, hemorrhagely can not spontaneously stop after Juvenile onset, spontaneous or slight wound; Thus at wound, haemophilia often of performing the operation, severe patient also can hematostaxis after comparatively aggravating activities.Primary disease is mainly hereditary, and current doctor trained in Western medicine there is no radical treatment, and base therapy measure is alternative medicine, and other drug all has temporary transient anastalsis.
Hemophilia lacks the difference of thrombin kind according to it, can be divided into:
(1). hemophilia A: be cause because thrombin eight (i.e. VIII) lacks, be also called hemophilia A, be modal hemophilia clinically, account for the 80%-85% of hemophilia number is even higher in some hotspot.
(2). hemophilia B: be cause because thrombin nine (i.e. IX) lacks, be also called hemophilia B, clinical rare compared with hemophilia A, account for about 15% of hemophilia number.
(3). hemophilia C: lack thrombin 11 (namely XI lacks, abroad also known as making Rosenthal syndrome).XI deficiency disease is extremely rare in China.
(4). acquired Hemophilia (namely acquired coagulation factor lacks): often because oneself factor causes some thrombin level to decline, or active reduction, as acquired thrombin eight (VIII) deficiency disease, often because self produces VIII factor antibody, cause coagulation disorders, cause acquired Hemophilia (hemophilia A).
Though without the title of " hemophilia " in motherland's traditional Chinese medical science medical science, surely belong to " blood disorder " or " disorders of blood " scope according to haemophiliachemophiliac syndrome.From "Nei Jing", ancient Chinese medicine doctor has more detailed elaboration to the syndrome of blood disorder, etiology and pathogenesis, Therapeutic Principle and square medicine.
Ming Dynasty's ZHANG Jie bin Jing-Yue Complete Works is discussed very detailed to blood disorder; " opinion controls blood disorder, must know that it is wanted, and blood dynamic by only fire, only gas.Examine fiery person, but examine its have fire without fire.The person that examines gas, but examine its deficiency of vital energy QI excess." Ming Dynasty's Zhao Xianke " Thorough Knowledge of Medicine " thinks as water true in kidney dries up, then kidney-yangly certainly will go up inflammation, blood also seethes with excitement with fire; As exhaustion kidney-yang in kidney, then true water is counter contains, and blood is also become homeless and to be depended on and upper general, and this is two kinds of pathological changes of blood disorder.Ming Dynasty's tassel Zhong Chun " Medical Notes in Xianxing Study" " further proposes and controls haematemesis three and want method: " should promoting the circulation of blood should not stop blooding, being suitable to invigorate the liver and not to reduce it, should the unsuitable pathogenic fire reducing of sending down the abnormal ascending QI ".
" blood is opinion no " in Qing Dynasty Tang Rong river proposes and controls blood four method: to stop blooding as first method, repercussive is the second method, and calming blood is the 3rd method, and tonify deficiency is the method receiving merit.
The traditional Chinese medical science thinks that the kidney being the origin of congenital constitution, main bone, and raw marrow, store essential substances, essence can change hemopoietic liquid.Therefore in the haemophiliachemophiliac process for the treatment of, the kidney invigorating should be prosecuted to the end, regulates the real basic principle for treatment primary disease of the equilibrium between yin and yang of kidney.
Haemophiliachemophiliac clinical manifestation mainly contains the following aspects:
(1) skin, mucosal bleeding: be hemorrhage multiple position.Visible have nasal bleeding, gums oozing of blood.Child's common forehead collision rift is hemorrhage or have hematoma, and the dermal ecchymosis, petechia are rare.Postextraction hemorrhage is often medical reason incessantly.
(2) arthrorrhagia: be one of the feature of primary disease.Show as in articular cavity and periarticular is organized hemorrhage.Be more common in Medium or severe type patient.Often occur in wound, walked for a long time, after strenuous exercise.Secondly many infringements knee joint and ankle joint are hip, elbow, shoulder, wrist and spinal column.Arthrorrhagia was divided into for three phases: first phase is acute stage, and main manifestations is the red and swollen heat pain in joint; Second phase is the panarthritis phase, is due to Repeated Hemorrhage, and blood absorption is incomplete, and form chronic inflammatory disease, synovial membrane thickens; Three phases and later stage, joint fibrosis or ankylosis, deformity, amyotrophy.So that function of joint is lost, there is knee joint flexing, turn up, fibula is subluxation shape in bone destruction, arthrogryposis.
(3) hemorrhage of muscle: be one of the most outstanding performance of hemophilia, all can occur everywhere at whole body.Can hematoma be formed when deep part muscle is hemorrhage, hematoma compression peripheral nerve, partial gall, limitation of activity can be occurred.Hematoma compression blood vessel then causes local blood circulation obstacle, and cause ischemic injury, fiber to change, severe patient can occur gangrene.
(4) hematuria: gross hematuria or microscopic hematuria can appear in heavy hemophiliac, how painless, without trauma history, if there is ureter clot to be formed, often with renal colic.
(5) other positions are hemorrhage: if having hematemesis, have blood in stool companion stomachache, be digestive tract hemorrhage; If hemoptysis, spitting of blood, hemothorax are that lung and breast abdomen are hemorrhage, more rare; Intracranial hemorrhage is less, and after craniocerebral injury of being everlasting occurs, and prognosis is not good, is often the cause of death.
Summary of the invention
Technical problem to be solved by this invention is, provides one to be used for the treatment of haemophiliachemophiliac Chinese medicine preparation and preparation method, and Chinese medicine has determined curative effect, rapid-action, safety is high, have no side effect and the simple advantage of medication.
In order to solve the problems of the technologies described above, the invention provides one and treat haemophiliachemophiliac Chinese medicine preparation, comprise following raw medicinal material: Corm Eleocharitis, Radix Rubiae, Cornu Bubali, the Radix Astragali, Herba stellariae mediae, Radix Codonopsis, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, Radix Rehmanniae, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae, Herba Artemisiae Annuae, Receptaculum Nelumbinis, Pseudobulbus Bletillae (Rhizoma Bletillae), broken close flower and Radix Glycyrrhizae.
Wherein, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material can be: Corm Eleocharitis 10 ~ 15 weight portion, Radix Rubiae 20 ~ 40 weight portion, Cornu Bubali 10 ~ 25 weight portion, the Radix Astragali 15 ~ 30 weight portion, Herba stellariae mediae 20 ~ 40 weight portion, Radix Codonopsis 20 ~ 30 weight portion, Herba Agrimoniae 15 ~ 30 weight portion, Herba Violae 20 ~ 35 weight portion, Radix Sophorae Tonkinensis 6 ~ 12 weight portion, Celastrus aceleatus Merr 15 ~ 25 weight portion, Radix Rehmanniae 20 ~ 30 weight portion, Rhizoma seu Herba Valerianae Tanguticae 10 ~ 20 weight portion, Rhizoma Imperatae 15 ~ 20 weight portion, Radix Ranunculi Ternati 10 ~ 20 weight portion, Placenta Hominis 20 ~ 30 weight portion, Cornu Saigae Tataricae 20 ~ 40 weight portion, Herba Artemisiae Annuae 10 ~ 30 weight portion, Receptaculum Nelumbinis 10 ~ 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 ~ 30 weight portion, brokenly closely spend 20 ~ 40 weight portions and Radix Glycyrrhizae 20 ~ 40 weight portion.
Wherein, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material can also be: Corm Eleocharitis 12 ~ 15 weight portion, Radix Rubiae 20 ~ 30 weight portion, Cornu Bubali 10 ~ 20 weight portion, the Radix Astragali 15 ~ 20 weight portion, Herba stellariae mediae 20 ~ 30 weight portion, Radix Codonopsis 20 ~ 25 weight portion, Herba Agrimoniae 20 ~ 30 weight portion, Herba Violae 20 ~ 30 weight portion, Radix Sophorae Tonkinensis 6 ~ 10 weight portion, Celastrus aceleatus Merr 20 ~ 25 weight portion, Radix Rehmanniae 20 ~ 25 weight portion, Rhizoma seu Herba Valerianae Tanguticae 16 ~ 20 weight portion, Rhizoma Imperatae 18 ~ 20 weight portion, Radix Ranunculi Ternati 10 ~ 16 weight portion, Placenta Hominis 20 ~ 26 weight portion, Cornu Saigae Tataricae 20 ~ 30 weight portion, Herba Artemisiae Annuae 20 ~ 30 weight portion, Receptaculum Nelumbinis 15 ~ 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 ~ 25 weight portion, brokenly closely spend 25 ~ 35 weight portions and Radix Glycyrrhizae 30 ~ 40 weight portion.
Wherein, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material can be again: Corm Eleocharitis 14 weight portion, Radix Rubiae 26 weight portion, Cornu Bubali 14 weight portion, the Radix Astragali 19 weight portion, Herba stellariae mediae 27 weight portion, Radix Codonopsis 23 weight portion, Herba Agrimoniae 26 weight portion, Herba Violae 22 weight portion, Radix Sophorae Tonkinensis 8 weight portion, Celastrus aceleatus Merr 21 weight portion, Radix Rehmanniae 24 weight portion, Rhizoma seu Herba Valerianae Tanguticae 19 weight portion, Rhizoma Imperatae 19 weight portion, Radix Ranunculi Ternati 14 weight portion, Placenta Hominis 23 weight portion, Cornu Saigae Tataricae 26 weight portion, Herba Artemisiae Annuae 28 weight portion, Receptaculum Nelumbinis 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 21 weight portion, brokenly closely spend 32 weight portions and Radix Glycyrrhizae 36 weight portion.
When the dosage form of described Chinese medicine preparation is syrup, its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, with the dissolve with ethanol of the determining alcohol 75% ~ 85% of 4 ~ 8 times relative to the mixture quality obtained, reflux was extracted after 4 ~ 6 hours, filter, obtain the first filtrate; By filtering residue again with the dissolve with ethanol of the determining alcohol 80% ~ 90% of 3 ~ 5 times relative to the mixture quality obtained, reflux was extracted after 2 ~ 4 hours, filtered, obtained the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.6 ~ 0.8g crude drug/mL, utilize the macroporous adsorptive resins eluting that volume is 5L, first use deionized water or the distilled water eluting of 5 times of resin column volumes, be 90% ~ 95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collect ethanol elution, concentrating under reduced pressure removing ethanol, drying is also ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, add and extract 2 ~ 4 times relative to the alcohol heating reflux of the determining alcohol 85% ~ 95% of 3 ~ 6 times of the mixture quality obtained, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, when the extracting solution after remove impurity being evaporated under the condition of 70 DEG C ~ 80 DEG C 70 DEG C, relative density is the mastic of 1.36 ~ 1.38, and the mastic spray dryer after concentrated is dried to powder;
3rd step, mixes surplus stock medical material in described ratio, adds the water of 6 ~ 10 times relative to the mixture quality obtained, and soaks 8-12 hour, and heating decoction 4 ~ 8 hours, filters; By filtering residue again with the water dissolution of 3 ~ 6 times relative to the mixture quality obtained, heating decoction 2 ~ 5 hours, filters; Merge the filtrate of twice, concentrating under reduced pressure removing moisture, drying is also ground into powder;
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, add the water dissolution relative to mixture quality 1.2 ~ 1.8 times, by 40% sodium hydroxide solution adjust ph to 7.0, stir evenly, cold preservation 60 hours ~ 72 hours under 3 DEG C ~ 6 DEG C conditions, filter, filtrate adds the sucrose relative to the mixture quality 0.5 ~ 0.8 times obtained, and stirs and dissolves, adjust ph to 7.0, add water appropriate, stir, leave standstill 12 ~ 18 hours, filter, fill, sterilizing, obtain syrup, the crude drug concentration in described syrup is 0.9g/mL ~ 1.2g/mL.
When the dosage form of described Chinese medicine preparation is oral solutions, its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that the methanol of 80% ~ 90% is as solvent with determining alcohol, adopt percolation with the speed of 1 ~ 3ml per minute slowly percolation, collect percolate, concentrating under reduced pressure is also dry, obtains and gets dry extract, be ground into powder; The quality of the methanol added is 4 ~ 6 times of first step mixture quality;
Second step, is mixed in proportion surplus stock medical material, is ground into powder;
3rd step, in the powder that second step obtains, adds the distilled water of the powder quality 5 ~ 8 times obtained relative to second step, decocts 3 ~ 6 hours, filter, obtain the first filtrate; Filtering residue is added the distilled water relative to its quality 3 ~ 6 times, decoct 2 ~ 4 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3 ~ 3/4 volume, add the ethanol that determining alcohol is 90 ~ 95%, make determining alcohol reach 70% ~ 80%, leave standstill 30 ~ 50 hours, filter, concentrating under reduced pressure, dry, obtain and get dry extract, be ground into powder;
4th step, the powder mixing first step and the 3rd step obtained, add the distilled water relative to powder quality 4 ~ 6 times amount, the sucrose of 0.4 ~ 0.8 times, the sodium benzoate of 0.02 ~ 0.05 times, stir, boil, cooling, filter, subpackage, namely sterilizing obtains oral liquid.
Beneficial effect of the present invention: the present invention is pure Chinese medicinal preparation, without any side effects, hemostasis and pain-relieving definite effect, rapid-action, therapeutic effect is good, taking convenience, long-term taking can not produce drug dependence, and raw material is easy to get, and drug cost is low, patient can be helped as early as possible to break away from whole blood and thrombin, alleviate the financial burden of patient treatment.
Detailed description of the invention
The invention provides one and treat haemophiliachemophiliac Chinese medicine preparation, comprise following raw medicinal material: Corm Eleocharitis, Radix Rubiae, Cornu Bubali, the Radix Astragali, Herba stellariae mediae, Radix Codonopsis, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, Radix Rehmanniae, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae, Herba Artemisiae Annuae, Receptaculum Nelumbinis, Pseudobulbus Bletillae (Rhizoma Bletillae), broken close flower and Radix Glycyrrhizae.
Wherein, in Chinese medicine preparation, the pharmacology of each raw medicinal material is as follows:
Corm Eleocharitis: sweet in the mouth; Cold in nature.Return lung; Stomach warp.Clearing away heat and promoting production of body fluid; Reduce phlegm; Removing food stagnancy.Main epidemic febrile disease is thirsty; Laryngopharynx swelling and pain; Phlegm-heat cough; Conjunctival congestion; Quench one's thirst; Dysentery; Jaundice; Pyretic stranguria; Food stagnation; Wart.
Radix Rubiae: bitter, cold.Return Liver Channel.Removing heat from blood, hemostasis, blood stasis dispelling, stimulates the menstrual flow.For spitting blood, epistaxis, metrorrhagia, traumatic hemorrhage, amenorrhea stasis blocking, arthralgia, tumbling and swelling.
Cornu Bubali: bitter, cold.GUIXIN, Liver Channel.Heat-clearing and toxic substances removing, removing heat from blood, arresting convulsion.For the high heat of epidemic febrile disease, unconsciousness and delirium, sends out speckle dermexanthesis, hematemesis and epistaxis, infantile convulsion, demented.
The Radix Astragali: sweet, temperature.Return lung, spleen channel.Invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.Weak for the deficiency of vital energy, anorexia and loose stool, sinking of QI of middle-JIAO, chronic diarrhea proctoptosis, metrorrhagia of having blood in stool, exterior deficiency spontaneous perspiration, deficiency of vital energy edema, carbuncle difficulty is burst, and burst for a long time and do not hold back, blood deficiency dull yellowish colored skin, interior-heat is quenched one's thirst; Chronic nephritis proteinuria, diabetes.
Herba stellariae mediae: micro-hardship, sweet, sour; Cool in nature.Return liver; Large intestine channel.Heat-clearing and toxic substances removing; Removing heat from blood eliminating carbuncle; Promoting blood circulation and stopping pain; Stimulating milk secretion; .Main dysentery; Intestinal carbuncle; Lung abscess; Acute mastitis; Furuncle swelling toxin; Swelling and pain of hemorrhoid; Hemorrhage; Traumatic pain; The stagnant stomachache of the stasis of blood in puerperal; Agalactia.
Radix Codonopsis: sweet is flat.Return spleen, lung meridian.Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.For deficiency of the spleen and lung, cardiopalmus of breathing hard, anorexia and loose stool, dyspnea due to deficiency is coughed, and interior-heat is quenched one's thirst.
Herba Agrimoniae: bitter in the mouth, puckery, property is put down.Return lung; Liver; Spleen channel.Astringing to arrest bleeding, dysentery relieving, parasite killing.Main spitting of blood; Spit blood; Hematuria; Have blood in stool; Dysentery; Bleeding not during menses; Impairment caused by overstrain takes off power; Carbuncle; Traumatic injury; Wound hemorrhage.
Herba Violae: bitter, acrid, cold.GUIXIN, Liver Channel.Heat-clearing and toxic substances removing, removing heat from blood is subsided a swelling.For furuncle swelling toxin, carbuncle carbuncle on the back, erysipelas, venom.
Radix Sophorae Tonkinensis: bitter, cold; Poisonous.Attach to the lung and stomach meridians.Heat-clearing and toxic substances removing, detumescence sore-throat relieving.Pent up for fire-toxin, laryngopharynx swelling and pain, gingivitis.
Celastrus aceleatus Merr: bitter; Pungent; Cool.Return liver; Gallbladder; Kidney channel.Heat-clearing and toxic substances removing; Expelling wind and removing dampness.Main air pain of dampness syndrome; Gout; Nephritis; Cholecystitis; Leukemia.
Radix Rehmanniae: sweet, cold.GUIXIN, liver, kidney channel.Clearing away heat and cooling blood, yin nourishing, promotes the production of body fluid.For calentura crimson tongue excessive thirst, yin asthenia generating intrinsic heat, hectic fever due to YIN-deficiency consumptive fever, interior-heat is quenched one's thirst, and spits blood, and epistaxis sends out speckle dermexanthesis.
Rhizoma seu Herba Valerianae Tanguticae: sweet in the mouth; Micro-pungent; Property is put down.Cough-relieving; Hemostasis; Dissipating blood stasis; Pain relieving.Main cough; Hemoptysis; Spit blood; Epistaxis; Metrostaxis; Rheumatic arthralgia; Fracture.
Rhizoma Imperatae: sweet, cold.Return lung, stomach, urinary bladder channel.Cooling blood for hemostasis, clearing away heat and promoting diuresis.Spit blood for heat in blood, epistaxis, hematuria, calentura excessive thirst, jaundice, edema, the puckery pain of pyretic stranguria; Acute nephritis edema.
Radix Ranunculi Ternati: sweet, acrid, warm.Return liver, lung meridian.Eliminating stagnation, detumescence., tuberculous lymphadenitis not routed for scrofula.
Placenta Hominis: sweet, one-tenth, temperature.GUIXIN, lung, kidney channel.The kidney warming mends essence, benefiting QI and nourishing blood.Win thin for asthenia, hectic fever due to YIN-deficiency night sweat, cough and asthma, lack of appetite is breathed hard, impotence and seminal emission, infertile hypogalactia.
Cornu Saigae Tataricae: become, cold.Return liver, heart channel.Suppressing the hyperactive liver to relieve the wind syndrome, liver heat removing and eyesight improving, loose blood removing toxic substances.For the hot infantile convulsion of height, coma convulsion is fainted, and eclamposia is twitched, and epilepsy is gone mad, and has a headache dizzy, cataract, maculae caused by violent heat pathogen, carbuncle sore tumefacting virus.
Herba Artemisiae Annuae: bitter, acrid, cold.Return liver, gallbladder meridian.Clearing away summer-heat, except steaming, preventing the attack (or recurrence) of malaria.For pathogenic summer-heat heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to YIN-deficiency consumptive fever, malaria cold and heat, jaundice due to damp-heat.
Receptaculum Nelumbinis: bitter, puckery, temperature.Return Liver Channel.Removing stasis to stop bleeding.For metrorrhagia, hematuria, bleeding hemorrhoids, postpartum stagnation, lochiorrhea.
Pseudobulbus Bletillae (Rhizoma Bletillae): bitter; Sweet; Puckery; Cold.Return lung; Stomach warp.Astringing to arrest bleeding; Detumescence and promoting granulation.Main spitting of blood; Spit blood; Epistaxis; Have blood in stool; Traumatic hemorrhage; Carbuncle pyogenic infections from tumour or sore; Scald and burn; Rhagadia manus et pedis; Anal fissure
Broken close flower: bitter in the mouth; Cool in nature.Removing toxic substances dysentery relieving; Synthetism is stopped blooding.Main toxic-heat and blood stasis; Traumatic injury is turned round and is improperly familiar with; Fracture; Traumatic hemorrhage.
Radix Glycyrrhizae: sweet, flat.GUIXIN, lung, spleen, stomach warp.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.For weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, the anxious pain of gastral cavity abdomen, extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strong.
Wherein, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material can be: Corm Eleocharitis 10 ~ 15 weight portion, Radix Rubiae 20 ~ 40 weight portion, Cornu Bubali 10 ~ 25 weight portion, the Radix Astragali 15 ~ 30 weight portion, Herba stellariae mediae 20 ~ 40 weight portion, Radix Codonopsis 20 ~ 30 weight portion, Herba Agrimoniae 15 ~ 30 weight portion, Herba Violae 20 ~ 35 weight portion, Radix Sophorae Tonkinensis 6 ~ 12 weight portion, Celastrus aceleatus Merr 15 ~ 25 weight portion, Radix Rehmanniae 20 ~ 30 weight portion, Rhizoma seu Herba Valerianae Tanguticae 10 ~ 20 weight portion, Rhizoma Imperatae 15 ~ 20 weight portion, Radix Ranunculi Ternati 10 ~ 20 weight portion, Placenta Hominis 20 ~ 30 weight portion, Cornu Saigae Tataricae 20 ~ 40 weight portion, Herba Artemisiae Annuae 10 ~ 30 weight portion, Receptaculum Nelumbinis 10 ~ 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 ~ 30 weight portion, brokenly closely spend 20 ~ 40 weight portions and Radix Glycyrrhizae 20 ~ 40 weight portion.
Wherein, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material can also be: Corm Eleocharitis 12 ~ 15 weight portion, Radix Rubiae 20 ~ 30 weight portion, Cornu Bubali 10 ~ 20 weight portion, the Radix Astragali 15 ~ 20 weight portion, Herba stellariae mediae 20 ~ 30 weight portion, Radix Codonopsis 20 ~ 25 weight portion, Herba Agrimoniae 20 ~ 30 weight portion, Herba Violae 20 ~ 30 weight portion, Radix Sophorae Tonkinensis 6 ~ 10 weight portion, Celastrus aceleatus Merr 20 ~ 25 weight portion, Radix Rehmanniae 20 ~ 25 weight portion, Rhizoma seu Herba Valerianae Tanguticae 16 ~ 20 weight portion, Rhizoma Imperatae 18 ~ 20 weight portion, Radix Ranunculi Ternati 10 ~ 16 weight portion, Placenta Hominis 20 ~ 26 weight portion, Cornu Saigae Tataricae 20 ~ 30 weight portion, Herba Artemisiae Annuae 20 ~ 30 weight portion, Receptaculum Nelumbinis 15 ~ 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 ~ 25 weight portion, brokenly closely spend 25 ~ 35 weight portions and Radix Glycyrrhizae 30 ~ 40 weight portion.
Wherein, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material can be again: Corm Eleocharitis 14 weight portion, Radix Rubiae 26 weight portion, Cornu Bubali 14 weight portion, the Radix Astragali 19 weight portion, Herba stellariae mediae 27 weight portion, Radix Codonopsis 23 weight portion, Herba Agrimoniae 26 weight portion, Herba Violae 22 weight portion, Radix Sophorae Tonkinensis 8 weight portion, Celastrus aceleatus Merr 21 weight portion, Radix Rehmanniae 24 weight portion, Rhizoma seu Herba Valerianae Tanguticae 19 weight portion, Rhizoma Imperatae 19 weight portion, Radix Ranunculi Ternati 14 weight portion, Placenta Hominis 23 weight portion, Cornu Saigae Tataricae 26 weight portion, Herba Artemisiae Annuae 28 weight portion, Receptaculum Nelumbinis 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 21 weight portion, brokenly closely spend 32 weight portions and Radix Glycyrrhizae 36 weight portion.
When the dosage form of described Chinese medicine preparation is syrup, its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, with the dissolve with ethanol of the determining alcohol 75% ~ 85% of 4 ~ 8 times relative to the mixture quality obtained, reflux was extracted after 4 ~ 6 hours, filter, obtain the first filtrate; By filtering residue again with the dissolve with ethanol of the determining alcohol 80% ~ 90% of 3 ~ 5 times relative to the mixture quality obtained, reflux was extracted after 2 ~ 4 hours, filtered, obtained the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.6 ~ 0.8g crude drug/mL, utilize the macroporous adsorptive resins eluting that volume is 5L, first use deionized water or the distilled water eluting of 5 times of resin column volumes, be 90% ~ 95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collect ethanol elution, concentrating under reduced pressure removing ethanol, drying is also ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, add and extract 2 ~ 4 times relative to the alcohol heating reflux of the determining alcohol 85% ~ 95% of 3 ~ 6 times of the mixture quality obtained, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, when the extracting solution after remove impurity being evaporated under the condition of 70 DEG C ~ 80 DEG C 70 DEG C, relative density is the mastic of 1.36 ~ 1.38, and the mastic spray dryer after concentrated is dried to powder;
3rd step, mixes surplus stock medical material in described ratio, adds the water of 6 ~ 10 times relative to the mixture quality obtained, and soaks 8-12 hour, and heating decoction 4 ~ 8 hours, filters; By filtering residue again with the water dissolution of 3 ~ 6 times relative to the mixture quality obtained, heating decoction 2 ~ 5 hours, filters; Merge the filtrate of twice, concentrating under reduced pressure removing moisture, drying is also ground into powder;
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, add the water dissolution relative to mixture quality 1.2 ~ 1.8 times, by 40% sodium hydroxide solution adjust ph to 7.0, stir evenly, cold preservation 60 hours ~ 72 hours under 3 DEG C ~ 6 DEG C conditions, filter, filtrate adds the sucrose relative to the mixture quality 0.5 ~ 0.8 times obtained, and stirs and dissolves, adjust ph to 7.0, add water appropriate, stir, leave standstill 12 ~ 18 hours, filter, fill, sterilizing, obtain syrup, the crude drug concentration in described syrup is 0.9g/mL ~ 1.2g/mL.
When the dosage form of described Chinese medicine preparation is oral solutions, its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that the methanol of 80% ~ 90% is as solvent with determining alcohol, adopt percolation with the speed of 1 ~ 3ml per minute slowly percolation, collect percolate, concentrating under reduced pressure is also dry, obtains and gets dry extract, be ground into powder; The quality of the methanol added is 4 ~ 6 times of first step mixture quality;
Second step, is mixed in proportion surplus stock medical material, is ground into powder;
3rd step, in the powder that second step obtains, adds the distilled water of the powder quality 5 ~ 8 times obtained relative to second step, decocts 3 ~ 6 hours, filter, obtain the first filtrate; Filtering residue is added the distilled water relative to its quality 3 ~ 6 times, decoct 2 ~ 4 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3 ~ 3/4 volume, add the ethanol that determining alcohol is 90 ~ 95%, make determining alcohol reach 70% ~ 80%, leave standstill 30 ~ 50 hours, filter, concentrating under reduced pressure, dry, obtain and get dry extract, be ground into powder;
4th step, the powder mixing first step and the 3rd step obtained, add the distilled water relative to powder quality 4 ~ 6 times amount, the sucrose of 0.4 ~ 0.8 times, the sodium benzoate of 0.02 ~ 0.05 times, stir, boil, cooling, filter, subpackage, namely sterilizing obtains oral liquid.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 syrup of the present invention
Get Corm Eleocharitis 140g, Radix Rubiae 260g, Cornu Bubali 140g, Radix Astragali 190g, Herba stellariae mediae 270g, Radix Codonopsis 230g, Herba Agrimoniae 260g, Herba Violae 220g, Radix Sophorae Tonkinensis 80g, Celastrus aceleatus Merr 210g, Radix Rehmanniae 240g, Rhizoma seu Herba Valerianae Tanguticae 190g, Rhizoma Imperatae 190g, Radix Ranunculi Ternati 140g, Placenta Hominis 230g, Cornu Saigae Tataricae 260g, Herba Artemisiae Annuae 280g, Receptaculum Nelumbinis 200g, Pseudobulbus Bletillae (Rhizoma Bletillae) 210g, broken close colored 320g and Radix Glycyrrhizae 360g;
Its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in proportion, with the dissolve with ethanol of the determining alcohol 80% of 6 times relative to the mixture quality obtained, reflux was extracted after 5 hours, filter, obtain the first filtrate; By filtering residue again with the dissolve with ethanol of the determining alcohol 90% of 3 times relative to the mixture quality obtained, reflux was extracted after 3 hours, filtered, obtained the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.7g crude drug/mL, utilize the macroporous adsorptive resins eluting that volume is 5L, first use deionized water or the distilled water eluting of 5 times of resin column volumes, be 95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collect ethanol elution, concentrating under reduced pressure removing ethanol, drying is also ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in proportion, add and extract 3 times relative to the alcohol heating reflux of the determining alcohol 90% of 4 times of the mixture quality obtained, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, when the extracting solution after remove impurity being evaporated under the condition of 80 DEG C 70 DEG C, relative density is the mastic of 1.37, and the mastic spray dryer after concentrated is dried to powder;
3rd step, is mixed in proportion surplus stock medical material, adds the water of 8 times relative to the mixture quality obtained, soaks 10 hours, and heating decoction 7 hours, filters; By filtering residue again with the water dissolution of 5 times relative to the mixture quality obtained, heating decoction 3 hours, filters; Merge the filtrate of twice, concentrating under reduced pressure removing moisture, drying is also ground into powder;
4th step, the powder mixing first step, second step and the 3rd step obtained, adds the water dissolution relative to mixture quality 1.6 times, by 40% sodium hydroxide solution adjust ph to 7.0, stir evenly, cold preservation 65 hours under 4 DEG C of conditions, filter, filtrate adds the sucrose relative to the mixture quality 0.7 times obtained, and stirs and dissolves, adjust ph to 7.0, adds water appropriate, stirs, leave standstill 16 hours, filter, fill, sterilizing, obtain syrup, the crude drug concentration in described syrup is 1.0g/mL.
Embodiment 2 oral solutions of the present invention
Get Corm Eleocharitis 120g, Radix Rubiae 240g, Cornu Bubali 170g, Radix Astragali 130g, Herba stellariae mediae 290g, Radix Codonopsis 210g, Herba Agrimoniae 220g, Herba Violae 270g, Radix Sophorae Tonkinensis 90g, Celastrus aceleatus Merr 230g, Radix Rehmanniae 210g, Rhizoma seu Herba Valerianae Tanguticae 160g, Rhizoma Imperatae 200g, Radix Ranunculi Ternati 120g, Placenta Hominis 250g, Cornu Saigae Tataricae 210g, Herba Artemisiae Annuae 220g, Receptaculum Nelumbinis 160g, Pseudobulbus Bletillae (Rhizoma Bletillae) 240g, broken close colored 270g and Radix Glycyrrhizae 310g;
Its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae Cornu Saigae Tataricae and broken close flower are mixed in proportion, be that the methanol of 85% is as solvent with determining alcohol, adopt percolation with the speed of 2ml per minute slowly percolation, collect percolate, concentrating under reduced pressure is also dry, obtains and gets dry extract, be ground into powder; The quality of the methanol added is 5 times of first step mixture quality;
Second step, is mixed in proportion surplus stock medical material, is ground into powder;
3rd step, in the powder that second step obtains, adds the distilled water of the powder quality 6 times obtained relative to second step, decocts 4 hours, filter, obtain the first filtrate; Filtering residue is added the distilled water relative to its quality 4 times, decoct 3 hours, filter, obtain the second filtrate; Merge the first filtrate and the second filtrate, concentrate away the water of 2/3 volume, add the ethanol that determining alcohol is 90%, make determining alcohol reach 75%, leave standstill 35 hours, filter, concentrating under reduced pressure, dry, obtain and get dry extract, be ground into powder;
4th step, the powder mixing first step and the 3rd step obtained, add the distilled water relative to powder quality 5 times amount, the sucrose of 0.6 times, the sodium benzoate of 0.03 times, stir, boil, cooling, filter, subpackage, namely sterilizing obtains oral liquid.
The raw test of poison:
Application NIH mice 80, SPF level, male and female half and half, body weight 14 ~ 22g, carries out acute toxicity testing.Mice is divided into two groups at random, i.e. matched group and administration group, fasting 12 hours before experiment, by Chinese materia medica syrup prepared by embodiments of the invention 1, gavage, gavage volume is 40m1/kg (namely unit dosage form is 40g crude drug/kg), matched group gives normal saline, administration in 1 day 2 times, delivery time 6 hours, Continuous Observation 14 days after administration, and record toxic reaction and the death toll of mice.Experimental result shows: compare with matched group, and after administration, mice has no notable difference, experiment Continuous Observation 14 days, mouse systemic situation, ingest, drink water, body weight increases all normal.Mouse oral gavage syrup LD of the present invention
50> 40g crude drug/kg, every day, maximum dosage-feeding was 80g crude drug/kg/ day.Chinese medicine clinical application amount of the present invention in 10g crude drug/day/people, adult body weight in 60kg, average dosage is 0.167g crude drug/kg/ day.By weighing machine: the dosis tolerata of mice (average weight is in 20g) oral administration gavage Chinese medicine of the present invention is 479 times of quantity.Therefore Chinese medicine acute toxicity of the present invention is low, clinical drug safety.
Clinical data:
Case selection: get the 69 routine sufferers that in August, 2007 ~ in November, 2012 derives from the court patient, be male.Hemophilia A 62 example; Hemophilia B 7 example; The course of disease 4 ~ 22 years old, has obvious hematostaxis history, and how because oral cavity, muscle or arthrorrhagia are medical, 46 examples have family history.
Diagnostic criteria: the diagnostic criteria of hemophilia A: 1. clinical manifestation: male patient has or without family history.Accordance joins stealthy genetic development.Female homozygote type can occur, Ji Shaojian; Joint, muscle, deep tissue are hemorrhage, can be spontaneous.Joint Repeated Hemorrhage causes joint deformity, and deep tissue Repeated Hemorrhage causes false tumor (hematocele).2. lab testing: clotting time (test tube method) severe extends, and moderate can be normal, slight and Subclinical is normal.Activated partial clotting time, severe obviously extends, and can be corrected by normal fresh plasma and adsorbed plasma, slightly slightly prolongation or normal.Platelet count, bleeding time and blood clot retraction are normal, prothrombin time is normal.FVIII procoagulant activity (FVIII:C) reduces.3. order of severity typing: heavy: FVIII:C < 2%, muscle, joint and deep tissue are hemorrhage, joint deformity, false tumor.Medium-sized: FVIII:C2 ~ 5%, joint, muscle and deep tissue can be had hemorrhage, joint deformity, but lighter.Light-duty: FVIII:C6 ~ 25%, joint and hemorrhage of muscle are little, without joint deformity.Subclinical: FVIII:C26 ~ 45%, only at severe trauma or postoperative hemorrhage.The diagnostic criteria of hemophilia B: 1. clinical manifestation: same to hemophilia A.2. lab testing: APTT extends, and can be corrected by normal serum, but unlike normal sorption blood plasma correct, light-duty can be normal.Plasma F IX:C measures and reduces or lack.The same hemophilia A of order of severity typing.
Exclusion standard: von Willebrand's disease patient; And the coagulation factor deficiency to cause because of other reasons; The deficiency of coagulation factors caused as mouse killing medicine is poisoning is hemorrhage, anticoagulant as warfarin etc. cause hemorrhage.
Therapeutic Method: take syrup prepared by the embodiment of the present invention 1, each 5ml, every day 2 times continuous 7 days is 1 course for the treatment of.
The standard of curative effect evaluation: clinical cure: bleeding disappears, hematoma dissipates, and joint walking function recovers normal, and the indices such as laboratory examination APTT recover normal, FVIII:C or FIX:C returns at least 50%-60%.The indices such as effective: bleeding obviously improves, hematoma dissipates substantially, and joint walking function improves, laboratory examination APTT are close to normal, FVIII:C or FIX:C returns to 30%-40%.Invalid: indices is without improvement or improve less.
Therapeutic outcome: before and after treatment, leading indicator average detection the results are shown in Table 1.
Take Chinese medicine preparation of the present invention, in 69 routine patients, 66 examples (95.65%) patient can produce obvious hemostasis and pain-relieving effect in 24 hours;
After taking 1 course for the treatment of, the frequency of hematostaxis etc. obviously reduce, and the dermal ecchymosis disappears, and muscle power obviously strengthens;
After taking 2 courses for the treatment of, self coagulation factor activity of patient is improved significantly, wherein 36 examples (52.17%) patient no longer can need input whole blood and thrombin, clinical cure 33 example (47.83%), effective 32 examples (46.38%), invalid 4 examples (5.8%);
Take medicine after 3 courses for the treatment of, self coagulation factor activity of more than 80% patient returns to 45%-60%, wherein 59 examples (85.51%) patient no longer can need input whole blood and thrombin, clinical cure 54 example (78.26%), effective 14 examples (20.29%), invalid 1 example (1.45%);
Take 4 courses for the treatment of, self coagulation factor activity of more than 85% patient returns at least 50%-60%, patient no longer occurs that the position such as skin, gingiva, joint is hemorrhage, the lopsided joint function disturbance of patient's enlargement recovers gradually, degree of bending and stretching obviously strengthens, and the periarticular muscles of atrophy obviously recovers, clinical cure 65 example (94.20%), effective 4 examples (5.8%), invalid 0 example (0%).
Front and back leading indicator average detection result treated by table 1
As shown in Table 1, after treatment compared with before treatment, PT, APTT, TT and STGT shorten (P < 0.05), FVIII:C value and FIX:C and raise (P < 0.05), namely, after Chinese medicine preparation of the present invention treatment, indices can be made to return to normal range or close to normal range.
Model case
Case 1: Lee, man, 16 years old, suffer from hemophilia since the childhood, right knee joint swells and ache recurrent exerbation 4 years, and flexion deformity is squatted down difficulty 2 years, have a medical check-up: right arthroncus of knee joints, violent tenderness, joint week footpath 38cm (left side 35cm), friction sense (++), stretch 180 °, bent 65 °, FVIII:C2.67%, APTT83.25s.Take syrup prepared by the embodiment of the present invention 1, each 5m1, every day 2 times continuous 7 days is 1 course for the treatment of.Take 3 courses for the treatment of, coagulation factor activity returns to 51.38%, and the lopsided joint function disturbance of patient's enlargement obviously recovers, and degree of bending and stretching obviously strengthens, arthroncus and this disappearance of tenderness sensillary base.Continue 1 course for the treatment of of taking medicine, symptom disappears substantially, can be freely movable.Leave hospital, continue to take 1 course for the treatment of and consolidated.Follow up a case by regular visits to 1 year, stable disease, without recurrence.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (3)
1. the haemophiliachemophiliac Chinese medicine preparation for the treatment of, it is characterized in that, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material is: Corm Eleocharitis 10 ~ 15 weight portion, Radix Rubiae 20 ~ 40 weight portion, Cornu Bubali 10 ~ 25 weight portion, the Radix Astragali 15 ~ 30 weight portion, Herba stellariae mediae 20 ~ 40 weight portion, Radix Codonopsis 20 ~ 30 weight portion, Herba Agrimoniae 15 ~ 30 weight portion, Herba Violae 20 ~ 35 weight portion, Radix Sophorae Tonkinensis 6 ~ 12 weight portion, Celastrus aceleatus Merr 15 ~ 25 weight portion, Radix Rehmanniae 20 ~ 30 weight portion, Rhizoma seu Herba Valerianae Tanguticae 10 ~ 20 weight portion, Rhizoma Imperatae 15 ~ 20 weight portion, Radix Ranunculi Ternati 10 ~ 20 weight portion, Placenta Hominis 20 ~ 30 weight portion, Cornu Saigae Tataricae 20 ~ 40 weight portion, Herba Artemisiae Annuae 10 ~ 30 weight portion, Receptaculum Nelumbinis 10 ~ 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 ~ 30 weight portion, brokenly closely spend 20 ~ 40 weight portions and Radix Glycyrrhizae 20 ~ 40 weight portion,
The dosage form of described Chinese medicine preparation is syrup, and its preparation method comprises the following steps:
The first step, Corm Eleocharitis, Radix Rubiae, Cornu Bubali, Herba stellariae mediae, Herba Agrimoniae, Herba Violae, Radix Sophorae Tonkinensis, Celastrus aceleatus Merr, broken close flower and Radix Glycyrrhizae are mixed in described ratio, with the dissolve with ethanol of the determining alcohol 75% ~ 85% of 4 ~ 8 times relative to the mixture quality obtained, reflux is extracted for 4 ~ 6 hours, filter, obtain the first filtrate; By filtering residue again with the dissolve with ethanol of the determining alcohol 80% ~ 90% of 3 ~ 5 times relative to the mixture quality obtained, reflux is extracted for 2 ~ 4 hours, filters, obtains the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.6 ~ 0.8g crude drug/mL, utilize the macroporous adsorptive resins eluting that volume is 5L, first use deionized water or the distilled water eluting of 5 times of resin column volumes, be 90% ~ 95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collect ethanol elution, concentrating under reduced pressure removing ethanol, drying is also ground into powder;
Second step, Rhizoma seu Herba Valerianae Tanguticae, Rhizoma Imperatae, Radix Ranunculi Ternati, Placenta Hominis, Cornu Saigae Tataricae and Receptaculum Nelumbinis are mixed in described ratio, add and extract 2 ~ 4 times relative to the alcohol heating reflux of the determining alcohol 85% ~ 95% of 3 ~ 6 times of the mixture quality obtained, each time extracting solution merges, with the centrifugal remove impurity of tube centrifuge, when the extracting solution after remove impurity being evaporated under the condition of 70 DEG C ~ 80 DEG C 70 DEG C, relative density is the mastic of 1.36 ~ 1.38, and the mastic spray dryer after concentrated is dried to powder;
3rd step, mixes surplus stock medical material in described ratio, adds the water of 6 ~ 10 times relative to the mixture quality obtained, and soaks 8-12 hour, and heating decoction 4 ~ 8 hours, filters; By filtering residue again with the water of 3 ~ 6 times relative to the mixture quality obtained, heating decoction 2 ~ 5 hours, filters; Merge the filtrate of twice, concentrating under reduced pressure removing moisture, drying is also ground into powder;
4th step, the powder mixing that the first step, second step and the 3rd step are obtained, add the water dissolution relative to mixture quality 1.2 ~ 1.8 times, by 40% sodium hydroxide solution adjust ph to 7.0, stir evenly, cold preservation 60 hours ~ 72 hours under 3 DEG C ~ 6 DEG C conditions, filter, filtrate adds the sucrose relative to the mixture quality 0.5 ~ 0.8 times obtained, and stirs and dissolves, adjust ph to 7.0, add water appropriate, stir, leave standstill 12 ~ 18 hours, filter, fill, sterilizing, obtain syrup, the crude drug concentration in described syrup is 0.9g/mL ~ 1.2g/mL.
2. Chinese medicine preparation as claimed in claim 1, it is characterized in that, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material is: Corm Eleocharitis 12 ~ 15 weight portion, Radix Rubiae 20 ~ 30 weight portion, Cornu Bubali 10 ~ 20 weight portion, the Radix Astragali 15 ~ 20 weight portion, Herba stellariae mediae 20 ~ 30 weight portion, Radix Codonopsis 20 ~ 25 weight portion, Herba Agrimoniae 20 ~ 30 weight portion, Herba Violae 20 ~ 30 weight portion, Radix Sophorae Tonkinensis 6 ~ 10 weight portion, Celastrus aceleatus Merr 20 ~ 25 weight portion, Radix Rehmanniae 20 ~ 25 weight portion, Rhizoma seu Herba Valerianae Tanguticae 16 ~ 20 weight portion, Rhizoma Imperatae 18 ~ 20 weight portion, Radix Ranunculi Ternati 10 ~ 16 weight portion, Placenta Hominis 20 ~ 26 weight portion, Cornu Saigae Tataricae 20 ~ 30 weight portion, Herba Artemisiae Annuae 20 ~ 30 weight portion, Receptaculum Nelumbinis 15 ~ 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 20 ~ 25 weight portion, brokenly closely spend 25 ~ 35 weight portions and Radix Glycyrrhizae 30 ~ 40 weight portion.
3. Chinese medicine preparation as claimed in claim 1, it is characterized in that, in described Chinese medicine preparation, the ratio of weight and number of each raw medicinal material is: Corm Eleocharitis 14 weight portion, Radix Rubiae 26 weight portion, Cornu Bubali 14 weight portion, the Radix Astragali 19 weight portion, Herba stellariae mediae 27 weight portion, Radix Codonopsis 23 weight portion, Herba Agrimoniae 26 weight portion, Herba Violae 22 weight portion, Radix Sophorae Tonkinensis 8 weight portion, Celastrus aceleatus Merr 21 weight portion, Radix Rehmanniae 24 weight portion, Rhizoma seu Herba Valerianae Tanguticae 19 weight portion, Rhizoma Imperatae 19 weight portion, Radix Ranunculi Ternati 14 weight portion, Placenta Hominis 23 weight portion, Cornu Saigae Tataricae 26 weight portion, Herba Artemisiae Annuae 28 weight portion, Receptaculum Nelumbinis 20 weight portion, Pseudobulbus Bletillae (Rhizoma Bletillae) 21 weight portion, brokenly closely spend 32 weight portions and Radix Glycyrrhizae 36 weight portion.
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