CN103272477A - Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof - Google Patents
Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application of the compound microbial active filling material. Ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1 related in the invention are isolated bacteria obtained in a reactor for processing repugnant substances, such as H2S, in a water pollution control laboratory of the ecological environment research centre of the Chinese academy of sciences. The bacterial strains are preserved in the general microbial centre of the Chinese general microbial strain preservation and management committee. The preservation numbers are CGMCC No.7400 and CGMCC No.7399 respectively. The compound microbial active filling material disclosed by the invention has the advantages of being large in specific area, high in porosity, good in air permeability, low in resistance, high in bacterial cell loading capacity and less prone to run away; and because of being loaded with ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1, the compound microbial active filling material is difficult to decay and deform in a bioreactor for purifying waste gas, and therefore, the compound microbial active filling material can be used for a long time and is applied to complex processing conditions.
Description
Technical field
The invention belongs to the environmental improvement field, specifically is a kind of complex microorganism active filler that removes the sulfur-containing foul material and preparation method thereof and application.
Background technology
Can produce a large amount of odorous gas in processes such as sewage, Treatment of Sludge, garbage disposal and compost, sulphur-containing substances such as hydrogen sulfide, mercaptan, thioether, carbon disulfide are smelly material common in the odorous gas.Odorant spills in the atmosphere, along with the diffuses flow of air to everywhere, surrounding environment is polluted, health is produced harm.At present, the improvement of air pollution and foul smell causes that people more and more pay close attention to, and many countries have all formulated strict emission control standards.The discharging of pollutant in the strict control waste gas, and require enterprise before discharging pollutants, take corresponding control measures.China has made strict regulation to the discharging of volatile organic contaminant and odorant in the country's " odorant pollutant discharge standard " that came into effect in 1993 in (GB14554-93).
Compare with other physical chemistry waste gas processing method, bioremediation has advantages such as cost is low, easy and simple to handle, technology cleaning, non-secondary pollution because of it, and the nineties becomes the focus of research later on.Biological ratio juris is to utilize the biological oxidation of microorganism the odorant of sulfur-bearing to be converted into the process of sulfate or sulphur simple substance.
But the common apposition growth of microorganism is at carrier surface, needs when the operation bioreactor regularly to the carrier spraying nutrient solution to keep the required nutrient of microbial growth and moisture.In the process of spray, microorganism very easily comes off from carrier surface, flows out from the bioreactor of purifying exhaust air with unnecessary nutrient solution, causes loss, causes the problem of the treatment effect reduction of sulfur-containing foul material.And carrier adopts composting material, wood chip, bark, Raschig ring, haydite etc. usually.The specific area of carriers such as composting material, wood chip, bark is little, and packed density is big, and the pressure loss is big, and is perishable, and service life is short; Raschig ring, haydite smooth surface, microorganism is difficult for adhering to, easier loss.The inoculum of the bioreactor of purifying sulfur-containing foul material mostly is the activated sludge of sewage treatment plant, and the microorganism laundering period is long, and bioreactor starts slow.
Summary of the invention
The purpose of this invention is to provide a kind of complex microorganism active filler and preparation and application that removes the sulfur-containing foul material, this complex microorganism active filler specific area is big, the porosity height, and good permeability, resistance is little; Bacterial cell appendix amount height is difficult for running off; Appendix has human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1, has the efficient of higher degraded hydrogen sulfide; Packed density in the bioreactor of purifying exhaust air is low, and pressure is low, and complicated treatment conditions can be used and adapt to not perishable distortion for a long time.
In order to achieve the above object, the present invention is by the following technical solutions:
Remove the complex microorganism active filler of sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrum anthropi) SL1, deposit number is CGMCC No.7400, water germ (Aquamicrobium defluvii) SU1 deposit number is CGMCC No.7399.
Described filler is one or more materials in light porous polyurethane foamed blocks, granular activated carbon, volcanic rock, the perlite.
The mass ratio of described two kinds of microbial bacteria suspensions and light porous material is 50-300: 10.
Human pallid bacillus SL1 cell concentration is greater than 10 in the described complex microorganism active filler
6CFU/g, the cell concentration of water germ SU1 is greater than 10
6CFU/g.
Described complex microorganism active filler preparation process is as follows:
(1) inclined-plane is cultivated: cultivate preparation human pallid bacillus SL1 and water germ SU1 slant strains respectively;
Described human pallid bacillus SL1 slant medium consists of: glucose 0.5g, KH
2PO
42.0g, K
2HPO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.2g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 ℃ of sterilization 20min;
Described water germ SU1 slant medium consists of: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, NaHCO
31.0g, agar 15g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min.
(2) preparation of bacteria suspension: respectively human pallid bacillus SL1 inclined-plane thalline and the water germ SU1 inclined-plane thalline of step (1) preparation are pressed 5-20% inoculum concentration fermented and cultured step by step, separating the acquisition cell concentration is that 0.4-0.8g/L human pallid bacillus SL1 bacteria suspension and cell concentration are 0.4-0.8g/L water germ SU1 bacteria suspension;
The condition of described fermented and cultured is: 36 ℃ of cultivation temperature, incubation time 48h, ventilation 5-200L/min, dissolved oxygen＞2.0mg/L, tank interior pressure 0.03-0.10Mpa.
Consisting of of described human pallid bacillus SL1 liquid fermentation medium: glucose 0.5g, KH
2PO
42.0g, K
2HPO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.2g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, water 1000ml, pH value 6.8-7.2,121 ℃ of sterilization 20min;
Consisting of of described water germ SU1 liquid fermentation medium: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min;
After fermentation is finished zymotic fluid is placed refrigerated centrifuge, in 4 ℃, 2000-8000rpm, centrifugal concentrated 5-20min, abandoning supernatant, with no bacterial nutrient solution dilution, obtain cell concentration respectively is that 0.4-0.8g/L human pallid bacillus SL1 bacteria suspension and cell concentration are 0.4-0.8g/L water germ SU1 bacteria suspension to precipitation respectively;
Described human pallid bacillus SL1 does not have consisting of of bacterial nutrient solution: glucose 0.5g, KH
2PO
43.0g, K
2HPO
43.0g, NH
4H
2PO
40.5g, MgCl
26H
2O0.2g, FeCl
30.01g, water 1000ml, pH value 6.8-7.2; 121 ℃ of sterilization 20min;
Described water germ SU1 does not have consisting of of bacterial nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeCl
30.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min;
(3) preparation of complex microorganism bacteria suspension: the human pallid bacillus SL1 bacteria suspension of step (2) preparation is mixed by 1: 1 volume ratio with water germ SUl bacteria suspension, obtain the complex microorganism bacteria suspension; The thalline total concentration is 0.4-0.8g/L in the described complex microorganism bacteria suspension;
(4) preparation of complex microorganism active filler:
(a) the complex microorganism bacteria suspension that step (3) is prepared sprays the surface of the light porous material of packet or grain shape; (b) will have human pallid bacillus SL1 and the porous material piece (or particle) of water germ SU1 bacterial cell puts into the impeller rotation and mixes; (c) again to light porous material surface spraying complex microorganism bacteria suspension, obtain the complex microorganism active filler, 4 ℃ of preservations are standby;
Preferred repeating step (a)-(c) 2-3 time, making in material surface and the micropore all, appendix has bacterial cell, the active filler of acquisition complex microorganism;
Described light porous material is one or more in polyurethane foamed blocks, granular activated carbon, volcanic rock, the perlite;
Described light porous material density is 0.7-1.0kg/m
3, porosity is 50-96%, particle diameter is 5-50mm;
Described complex microorganism with the form appendix of bacteria suspension on filler;
Human pallid bacillus SL1 cell concentration is greater than 10 in the described complex microorganism active filler
6CFU/g
(activity is filled out Material), the cell concentration of water germ SU1 is greater than 10
6CFU/g
(active filler)
The invention provides human pallid bacillus (Ochrobactrum anthropi) SL1 (CGMCC No.7400) of strain degraded sulfur-containing foul material, by the microbial inoculum of this bacterium preparation, is 1.0-90m at gas flow
3/ h, time of staying 1-3min, temperature 20-37 ℃, concentration of hydrogen sulfide is 0.1-80mg/m3, methyl mercaptan 0.1-10mg/m3, methyl sulfide 0.1-10mg/m3 can reach more than 90% sulfur-containing foul mass degradation efficient such as hydrogen sulfide, methyl mercaptan, methyl sulfides.The gas that can be used for the purifying sulfur-containing foul material solves the odor pollution problem that processes such as sewage, Treatment of Sludge, garbage disposal and compost produce.
Human pallid bacillus involved in the present invention (Ochrobactrum anthropi) SL1 bacterial strain characteristics are as follows:
This bacterial strain colony colour on the LB solid plate is colourless, circle, and smooth surface, neat in edge, moistening.It is shaft-like that thalline is, and size is 0.5-0.8 * 3.0-3.5 μ m, peritrichous, Gram-negative, catalase and oxidase positive.The indoles feminine gender.Glucose fermentation produces acid, can utilize nitrate, not hydrolysis aesculin and gelatin.Can extensively utilize carbohydrate, amino acid, organic acid to be carbon source.Optimum growth temperature is 20-37 ℃.
Adopting full-automatic magnetic bead nucleic acid extraction instrument Smart LabAssist-16 and respective environment sample DNA to extract purification kit (Taiwan round dot how rice technical concern Co., Ltd) extracts and purifying purpose bacterial strain DNA respectively, select for use bacterium universal primer F16S-27 and R16S-1492 to carry out pcr amplification, primer sequence is respectively:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
The PCR reaction condition is set at: 94 ℃ of pre-sex change 5min; 94 ℃ of sex change 30sec subsequently, 56 ℃ of annealing 30sec, 72 ℃ are extended 90sec, circulate 25 cycles; 72 ℃ are extended 10min then; Last 4 ℃ keep 10min.Then the PCR product is connected on the PMD-19T carrier, is transformed into subsequently in the bacillus coli DH 5 alpha competent cell.Competent cell 50 μ l after transforming are coated on the LB culture medium that contains galactoside, isopropyl-β-d-thiogalactoside and ampicillin.Cultivate after 12 hours, carry out liquid LB medium culture from 25 single bacterial plaques of white of each dull and stereotyped picking.Cultivate after 2 hours, nutrient solution is carried out sequencing analysis.The sequence that records at first adopts DNAMAN software to carry out the similitude merger, be an operating unit with similitude greater than 97% sequence merger, the representative sequence of choosing in each operating unit utilizes the 16SrDNA gene order in BLAST and the GenBank database to carry out homology analysis, choose the above length of 1400bp and compare (Clustelxl.83), adopt the ortho position to connect (Neighbour Joining) method and carry out systematics analysis (MEGA3.1).Phylogenetic tree as shown in Figure 1.
This microbial inoculum can prepare by the production technology of test tube slant kind-shake bottle a kinds-seeding tank-production jar-product (packing formulation is liquid bacterial agent or solid absorption microbial inoculum).
The invention provides water germ (Aquamicrobium defluvii) SU1 (CGMCC No.7399) of strain degraded sulfur-containing foul material, the microbial inoculum that is prepared by this bacterium is 1.0-50m at gas flow
3/ h, time of staying 1-3min, temperature 20-37 ℃, concentration of hydrogen sulfide is 0.1-50mg/m
3, methyl mercaptan 0.1-20mg/m
3, can reach more than 85% sulfur-containing foul mass degradation efficient such as hydrogen sulfide, methyl mercaptans.The gas that can be used for the purifying sulfur-containing foul material solves the odor pollution problem that processes such as sewage, Treatment of Sludge, garbage disposal and compost produce.
Water germ SU1 bacterial strain characteristics involved in the present invention are as follows:
This bacterial strain colony colour on the LB solid plate is white, and is opaque, circle, and the edge is smooth, and is moistening.Thalline is rod-short, and size is 0.5-0.8 * 1.5-2.0 μ m, and amphitrichous is movable, Gram-negative, catalase and oxidase positive.Glucose fermentation produces acid, can utilize nitrate, not hydrolysis lysine, arginine and gelatin.Utilize carbohydrate, organic acid to be carbon source on a small quantity.Optimum growth temperature is 25-37 ℃.
Adopting full-automatic magnetic bead nucleic acid extraction instrument Smart LabAssist-16 and respective environment sample DNA to extract purification kit extracts and purifying purpose bacterial strain DNA respectively, select for use bacterium universal primer F16S-27 and R16S-1492 to carry out pcr amplification, primer sequence is respectively:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
The PCR reaction condition is set at: 94 ℃ of pre-sex change 5min; 94 ℃ of sex change 30sec subsequently, 56 ℃ of annealing 30sec, 72 ℃ are extended 90sec, circulate 25 cycles; 72 ℃ are extended 10min then; Last 4 ℃ keep 10min.Then the PCR product is connected on the PMD-19T carrier, is transformed into subsequently in the bacillus coli DH 5 alpha competent cell.Competent cell 50 μ l after transforming are coated on the LB culture medium that contains galactoside, isopropyl-β-d-thiogalactoside and ampicillin.Cultivate after 12 hours, carry out liquid LB medium culture from 25 single bacterial plaques of white of each dull and stereotyped picking.Cultivate after 2 hours, nutrient solution is carried out sequencing analysis.The sequence that records at first adopts DNAMAN software to carry out the similitude merger, be an operating unit with similitude greater than 97% sequence merger, the representative sequence of choosing in each operating unit utilizes the 16SrDNA gene order in BLAST and the GenBank database to carry out homology analysis, choose the above length of 1400bp and compare (Clustelxl.83), adopt the ortho position to connect (Neighbour Joining) method and carry out systematics analysis (MEGA3.1).Phylogenetic tree as shown in Figure 2.
This microbial inoculum can prepare by the production technology of test tube slant kind-shake bottle a kinds-seeding tank-production jar-product (packing formulation is liquid bacterial agent or solid absorption microbial inoculum).
Another object of the present invention is to utilize the complex microorganism active filler to carry out odorous gas to handle, particularly at degraded H
2Application in the S gas, concrete steps are as follows:
(1) appendix there is the complex microorganism active filler of human pallid bacillus SL1 and water germ SU1 place a container, feeds H
2S gas is with H
2S gas is the sulphur source, the active filler of activation complex microorganism; Soak time is 1-14 days;
Described H
2The concentration of S is 50-200ppm;
(2) the complex microorganism active filler after will activating places bioreactor, feed the gas of sulfur-containing foul material, with the sulfur-containing foul material in human pallid bacillus SL1 and the water germ SU1 filler purifying exhaust air, complex microorganism active filler volume is 0.001-200m
3, the total concentration of sulfide is 50-200ppm, gas flow rate is 0.05-10000m
3/ h, purification temperature are 10-35 ℃; Regularly keep the activity of complex microorganism active filler to complex microorganism active filler surface spraying nutrient solution, the spray frequency sprayed 1 time for 1-7 days, and each spray rate is 1.0-200L/min, and each spray time is 20-120min;
Consisting of of described nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, K
2HPO
42.0g, KNO
32.0g, NH
4C10.5g, MgCl
26H
2O0.2g, FeSO
47H
2O0.01g, water 1000ml, pH6.8-7.2; Follow the tracks of the degradation capability that detects the sulfur-containing foul material.
The present invention adopts gas chromatography to follow the tracks of and detects sulfur-containing compound; Described gas chromatography is: adopt Agilent GC-6890N gas chromatograph assay determination sulfide concentration, chromatographic column is DB-1701 capillary column (30m * 0.32mm * 0.25 μ m), vaporizer, FPD detector, column temperature are set to 100 ℃, 250 ℃, 220 ℃ respectively, sample size 10 μ L, the N2 flow velocity is 0.3mL/min, and total flow is 12mL/min.
Human pallid bacillus involved in the present invention (Ochrobactrum anthropi) SLl and water germ (Aquamicrobium defluvii) SU1 contain H for handling from Ecological Environment Research Center, Chinese Academy of Sciences water pollution control laboratory
2Obtain isolate in the reactor of odorants such as S, oneself is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 02nd, 2013, deposit number is respectively CGMCCNo.7400 and CGMCC No.7399, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.
Beneficial effect:
1. have superiority bacterium human pallid bacillus SL1 and water germ SU1 of complex microorganism active filler appendix of the present invention has the efficient of higher degraded hydrogen sulfide; And the laundering period of dominant bacteria is short, can begin degradation reaction fast, is applied in the waste gas bioreactor of purifying sulfur-containing foul material, can shorten the starting period.
Single bacterium only has higher removal effect to a kind of sulfur-containing foul material, and when being mixed with multiple sulfur-containing foul material in the gas, clearance obviously descends.Two kinds of bacterium mix the mist that can effectively handle multiple odorants such as containing hydrogen sulfide, mercaptan, thioether.And the concentration of sulfur-containing foul material is higher in gas, greater than 100mg/m
3The time, clearance still can keep more than 90%.
2. utilize sedimentation, adhere to the effect with multiple mechanics such as centrifugal, to degrade dominant bacteria human pallid bacillus SL1 and water germ SU1 appendix on light porous material, form the complex microorganism active filler, bacterial cell not only can be attached to filling surface equably, and can enter in the hole of filler inside, make the bacterial cell appendix amount height of complex microorganism active filler, be difficult for from bioreactor, running off.
3. through overactivation, bioreactor is started fast before the complex microorganism active filler is used.The bacterial cell enzyme of appendix is lived stable, can keep the clean-up effect of sulfur-containing foul material steady in a long-term.
4. be used for rough surface, the porous of the carrier material of complex microorganism active filler, be suitable for bacterial cell and adhere to; Specific area is big, good permeability, and resistance is little, is conducive to bacterial cell and contacts with sulfur-containing foul material in the waste gas, improves mass-transfer efficiency.
5. be used for the carrier material light weight of complex microorganism active filler, be applied in the waste gas bioreactor of purifying sulfur-containing foul material, packed density is low, and the pressure loss is little.
6. the physical condition of multiple complexity can be used and adapt to the not perishable distortion of complex microorganism active filler for a long time.
Description of drawings
Fig. 1 human pallid bacillus (Ochrobactrum anthropi) SL1 phylogenetic tree
Fig. 2 water germ (Aquamicrobium defluvii) SU1 phylogenetic tree
The specific embodiment
Example 1: a kind of microbial activity filler that removes the sulfur-containing foul material and preparation method thereof.
A kind of complex microorganism active filler that removes the sulfur-containing foul material, described filler appendix have human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1; Described human pallid bacillus (Ochrobactrum anthropi) SL1, deposit number is CGMCC No.7400, water germ (Aquamicrobium defluvii) SU1 deposit number is CGMCC No.7399;
Described filler is polyurethane foamed blocks;
Described packing density is 0.8kg/m
3, porosity is 86%, particle diameter is 30mm;
Described complex microorganism with the form appendix of bacteria suspension on filler;
The mass ratio of described complex microorganism bacteria suspension and polyurethane foamed blocks is 100: 10;
Human pallid bacillus SL1 cell concentration is greater than 10 in the described complex microorganism active filler
6CFU/g, the cell concentration of water germ SU1 is greater than 10
6CFU/g.
Described complex microorganism active filler preparation process is as follows:
(1) inclined-plane is cultivated: cultivate preparation human pallid bacillus SL1 and water germ SU1 slant strains respectively;
Described human pallid bacillus SL1 slant medium consists of: glucose 0.5g, KH
2PO
42.0g, K
2HPO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.2g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,121 ℃ of sterilization 20min;
Described water germ SU1 slant medium consists of: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, NaHCO
31.0g, agar 15g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min.
(2) preparation of bacteria suspension: respectively human pallid bacillus SL1 inclined-plane thalline and the water germ SU1 inclined-plane thalline of step (1) preparation are pressed 10% inoculum concentration fermented and cultured step by step, separating the acquisition cell concentration is that 0.6g/L human pallid bacillus SL1 bacteria suspension and cell concentration are 0.5g/L water germ SU1 bacteria suspension;
Human pallid bacillus SL1 bacteria suspension and water germ SU1 bacteria suspension preparation method are as follows;
Respectively human pallid bacillus SL1 inclined-plane thalline and the water germ SU1 inclined-plane thalline of step (1) preparation are pressed 10% inoculum concentration fermented and cultured step by step, 36 ℃ of incubation time 48h of cultivation temperature, ventilation 100L/min, dissolved oxygen＞2.0mg/L, tank pressure 0.06Mpa, zymotic fluid places refrigerated centrifuge in 4 ℃, 5000rpm, centrifugal concentrated 10min, abandoning supernatant, with no bacterial nutrient solution dilution, obtain cell concentration respectively is that 0.5g/L human pallid bacillus SL1 bacteria suspension and cell concentration are 0.5g/L water germ SU1 bacteria suspension to precipitation respectively;
Consisting of of described human pallid bacillus SL1 liquid fermentation medium: glucose 0.5g, KH
2PO
42.0g, K
2HPO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.2g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, water 1000ml, pH value 6.8-7.2,121 ℃ of sterilization 20min;
Consisting of of described water germ SU1 liquid fermentation medium: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeSO
47H
2O0.01g, Na
2S
2O
35H
2O5.0g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min;
Described human pallid bacillus SL1 does not have consisting of of bacterial nutrient solution: glucose 0.5g, KH
2PO
43.0g, K
2HPO
43.0g, NH
4H
2PO
40.5g, MgCl
26H
2O0.2g, FeCl
30.01g, water 1000ml, pH value 6.8-7.2; 121 ℃ of sterilization 20min;
Described water germ SU1 does not have consisting of of bacterial nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeCl
30.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min;
(3) preparation of complex microorganism bacteria suspension: the human pallid bacillus SL1 bacteria suspension of step (2) preparation is mixed by 1: 1 volume ratio with water germ SU1 bacteria suspension, obtain the complex microorganism bacteria suspension; The thalline total concentration is 0.5g/L in the described complex microorganism bacteria suspension;
(4) preparation of complex microorganism active filler: (a) the complex microorganism bacteria suspension that step (3) is prepared sprays the surface of the light porous material of packet or grain shape by pump and spray head; Spray rate is 10L/min, and spray time is 60min; Stir simultaneously, mixing speed is 100rpm, and mixing time is 100min; Bacteria suspension is fully contacted with light porous; (b) the porous material piece (or particle) that will have human pallid bacillus SL1 and water germ SU1 bacterial cell is put into impeller, and rotation mixes 50min under the 10rpm rotating speed; Along with the rotation of blender, be attached to the bacterial cell on light porous material surface because action of centrifugal force enters into hole inside; (c) again to light porous material surface spraying complex microorganism bacteria suspension, stir simultaneously; Obtain the complex microorganism active filler, 4 ℃ of preservations are standby;
Repeating step (a)-(c) 3 times makes in material surface and the micropore all that appendix has bacterial cell, obtains the complex microorganism active filler;
Described rotary blender is efficient meticulous container revolution, agitating type mixing apparatus, is used for the dryness of different proportion or the even mixing of moist particle, and does not produce fusion, the volatilization or rotten of material in mixed process.The rotary blender main body is that stainless steel material is made, and volume is 0.05-10m
3, inside and outside wall polishing, no dead angle, built-in stirring vane, external rotating adds that built-in blade stirs, light porous filler and bacteria suspension radially roll in impeller, simultaneously also can be laterally and convection current up and down, the mixing efficiency height.In the mixer rotary course, on the one hand each component substances is fully mixed, on the other hand, because action of centrifugal force, bacterial cell can enter in the hole of porous material inside, not only makes the bacterial cell appendix amount height of complex microorganism active filler, and bacterial cell is difficult for running off.
Example 2: a kind of microbial activity filler is at degraded H
2Application in the S gas.
Another object of the present invention is to utilize the complex microorganism active filler to carry out odorous gas to handle, particularly at degraded H
2Application in the S gas, concrete steps are as follows:
(1) appendix there is the complex microorganism active filler of human pallid bacillus SL1 and water germ SU1 place a container, feeds H
2S gas is with H
2S gas is the sulphur source, the active filler of activation complex microorganism; Soak time is 7 days;
Described H
2The concentration of S is 100ppm;
(2) the complex microorganism active filler after will activating places bioreactor, feeds the gas of sulfur-containing foul material, and with the sulfur-containing foul material in human pallid bacillus SL1 and the water germ SU1 purifying exhaust air, complex microorganism active filler volume is 100m
3, the total concentration of sulfide is 100ppm, gas flow rate is 1000m
3/ h, purification temperature are 25 ℃; Regularly keep the activity of complex microorganism active filler to complex microorganism active filler surface spraying nutrient solution, the spray frequency is 3 days sprays 1 time, and each spray rate is 50L/min, and each spray time is 60min;
Consisting of of described nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, K
2HPO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.2g, FeSO
47H
2O0.01g, water 1000ml, pH6.8-7.2; Follow the tracks of the degradation capability that detects the sulfur-containing foul material.
Example 3: result of use experiment (filler of preparation in the example 1 is carried out following three experiments respectively).
(1) the dispose of sewage gases containing stench sulphureous gases of factory's mudpan loss, gas flow is 35m
3/ h, the time of staying is 1～3mins, operates under the room temperature condition.The inlet gas concentration of hydrogen sulfide is: 14.86mg/m
3, the concentration of giving vent to anger is 0.03mg/m
3, being lower than the odorant pollutant discharge standard that country formulates, clearance reaches 99%.
(2) gases containing stench sulphureous gases that produces for the treatment of sludge composting, the concentration of hydrogen sulfide and ethyl mercaptan is respectively 18mg/m
3And 2.53mg/m
3, gas flow is 70m
3/ h, the time of staying is 1～3mins, operates under the room temperature condition, the clearance of hydrogen sulfide and ethyl mercaptan is all greater than 90%.
(3) refuse landfill is handled and is contained 162.1mg/m
3Hydrogen sulfide, 3.82mg/m
3Methyl mercaptan, and 8.2mg/m
3The mix waste gas of methyl sulfide, gas flow are 60m
3/ h, the time of staying is 1～3mins, temperature 18-37 ℃, the clearance of hydrogen sulfide, methyl mercaptan, methyl sulfide reaches 95.1%, 90.6% and 91% respectively.
Example 4 is substantially with example 1
Remove the complex microorganism active filler of sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrum anthropi) SL1, deposit number is CGMCC No.7400, water germ (Aquamicrobium defluvii) SU1 deposit number is CGMCC No.7399.
Described filler is granular activated carbon.
The mass ratio of described two kinds of microbial bacteria suspensions and light porous material is 200: 10.
Human pallid bacillus SL1 cell concentration is greater than 10 in the described complex microorganism active filler
6CFU/g, the cell concentration of water germ SU1 is greater than 10
6CFU/g.
Described complex microorganism active filler preparation process is with example 1:
The thalline total concentration is 0.4g/L in the described complex microorganism bacteria suspension;
Example 5
Remove the complex microorganism active filler of sulfur-containing foul material, described filler appendix has human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrum anthropi) SL1, deposit number is CGMCC No.7400, water germ (Aquamicrobium defluvii) SU1 deposit number is CGMCC No.7399.
Described filler is perlite.
The mass ratio of described two kinds of microbial bacteria suspensions and light porous material is 50: 10.
Human pallid bacillus SL1 cell concentration is greater than 10 in the described complex microorganism active filler
6CFU/g, the cell concentration of water germ SU1 is greater than 10
6CFU/g.
Described complex microorganism active filler prepares with example 1:
The thalline total concentration is 0.8g/L in the described complex microorganism bacteria suspension;
Described light porous material density is 0.7kg/m3, and porosity is 50%, and particle diameter is 5-20mm.
Claims (8)
1. complex microorganism active filler that removes the sulfur-containing foul material, described filler appendix have human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1 bacteria suspension; Described human pallid bacillus (Ochrobactrum anthropi) SL1, deposit number is CGMCC No.7400, water germ (Aquamicrobium defluvii) SU1 deposit number is CGMCC No.7399.
2. according to the described a kind of complex microorganism active filler that removes the sulfur-containing foul material of claim 1, described filler is one or more materials in light porous polyurethane foamed blocks, granular activated carbon, volcanic rock, the perlite.
3. according to the described a kind of complex microorganism active filler that removes the sulfur-containing foul material of claim 1, the mass ratio of described two kinds of microbial bacteria suspensions and light porous material is 50-300: 10.
4. according to the described a kind of complex microorganism active filler that removes the sulfur-containing foul material of claim 1, human pallid bacillus SL1 cell concentration is greater than 10 in the described complex microorganism active filler
6CFU/g, the cell concentration of water germ SU1 is greater than 10
6CFU/g.
5. according to the described a kind of preparation method who removes the complex microorganism active filler of sulfur-containing foul material of claim 1, step is as follows:
(1) inclined-plane is cultivated: cultivate preparation human pallid bacillus (Ochrobactrum anthropi) SL1 and water germ (Aquamicrobium defluvii) SU1 slant strains respectively;
(2) preparation of bacteria suspension: respectively human pallid bacillus SL1 inclined-plane thalline and the water germ SU1 inclined-plane thalline of step (1) preparation are pressed 5-20% inoculum concentration fermented and cultured step by step, separating the back is that 0.4-0.8g/L human pallid bacillus SL1 bacteria suspension and cell concentration are 0.4-0.8g/L water germ SU1 bacteria suspension with no bacterial nutrient solution dilution acquisition cell concentration;
(3) preparation of complex microorganism bacteria suspension: the human pallid bacillus SL1 bacteria suspension of step (2) preparation is mixed by 1: 1 volume ratio with water germ SU1 bacteria suspension, obtain the complex microorganism bacteria suspension; The thalline total concentration is 0.4-0.8g/L in the described complex microorganism bacteria suspension;
(4) preparation of complex microorganism active filler:
(a) the complex microorganism bacteria suspension that step (3) is prepared sprays the surface of the light porous material of packet or grain shape; (b) will have human pallid bacillus SL1 and the porous material piece of water germ SU1 bacterial cell puts into the impeller rotation and mixes; (c) again to light porous material surface spraying complex microorganism bacteria suspension, obtain the complex microorganism active filler, 4 ℃ of preservations are standby; Preferred repeating step (a)-(c) 2-3 time, making in material surface and the micropore all, appendix has bacterial cell, the active filler of acquisition complex microorganism.
6. according to the described a kind of preparation method who removes the complex microorganism active filler of sulfur-containing foul material of claim 1-5, described water germ SU1 does not have consisting of of bacterial nutrient solution: glucose 0.5g, dusty yeast 0.2g, KH
2PO
42.0g, KNO
32.0g, NH
4Cl0.5g, MgCl
26H
2O0.5g, FeCl
30.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-6.5,121 ℃ of sterilization 20min.
7. according to the described a kind of preparation method who removes the complex microorganism active filler of sulfur-containing foul material of claim 1-5, described human pallid bacillus SL1 does not have consisting of of bacterial nutrient solution: glucose 0.5g, KH
2PO
43.0g, K
2HPO
43.0g, NH
4H
2PO
40.5g, MgCl
26H
2O0.2g, FeCl
30.01g, water 1000ml, pH value 6.8-7.2; 121 ℃ of sterilization 20min.
8. according to the described a kind of application of complex microorganism active filler in removing the sulfur-containing foul material that removes the sulfur-containing foul material of claim 1-7.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103432900A (en) * | 2013-09-07 | 2013-12-11 | 中蓝连海设计研究院 | Application of microbial agent to malodorous gas pollution regulation |
| CN103691305A (en) * | 2013-12-18 | 2014-04-02 | 中国科学院生态环境研究中心 | Preparation method of Thermus-containing active filler |
| CN104531249A (en) * | 2014-12-31 | 2015-04-22 | 山东圣琪生物有限公司 | Methane desulfurizer |
| CN104667475A (en) * | 2014-12-16 | 2015-06-03 | 华南农业大学 | Method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropi |
| CN107011912A (en) * | 2017-04-12 | 2017-08-04 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321549A (en) * | 2011-08-01 | 2012-01-18 | 浙江工业大学 | Composite microbial inoculum, immobilization method and application thereof |
| CN102965294A (en) * | 2011-10-25 | 2013-03-13 | 北京农业生物技术研究中心 | Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application |
-
2013
- 2013-05-29 CN CN201310205399.2A patent/CN103272477B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321549A (en) * | 2011-08-01 | 2012-01-18 | 浙江工业大学 | Composite microbial inoculum, immobilization method and application thereof |
| CN102965294A (en) * | 2011-10-25 | 2013-03-13 | 北京农业生物技术研究中心 | Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103432900A (en) * | 2013-09-07 | 2013-12-11 | 中蓝连海设计研究院 | Application of microbial agent to malodorous gas pollution regulation |
| CN103432900B (en) * | 2013-09-07 | 2015-08-12 | 中蓝连海设计研究院 | The purposes of a kind of microbial bacterial agent in foul gas pollution control |
| CN103691305A (en) * | 2013-12-18 | 2014-04-02 | 中国科学院生态环境研究中心 | Preparation method of Thermus-containing active filler |
| CN103691305B (en) * | 2013-12-18 | 2016-03-30 | 中国科学院生态环境研究中心 | A kind of preparation method of the active filler containing the hot bacterium that dwells |
| CN104667475A (en) * | 2014-12-16 | 2015-06-03 | 华南农业大学 | Method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropi |
| CN104667475B (en) * | 2014-12-16 | 2017-09-26 | 华南农业大学 | A kind of method of utilization anthropi efficient degradation Fluoxastrobin |
| CN104531249A (en) * | 2014-12-31 | 2015-04-22 | 山东圣琪生物有限公司 | Methane desulfurizer |
| CN107011912A (en) * | 2017-04-12 | 2017-08-04 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
| CN107011912B (en) * | 2017-04-12 | 2019-10-25 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
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