CN107011912A - Pesticide carbendazim microbial inoculum for degrading and preparation method thereof - Google Patents
Pesticide carbendazim microbial inoculum for degrading and preparation method thereof Download PDFInfo
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- CN107011912A CN107011912A CN201710237215.9A CN201710237215A CN107011912A CN 107011912 A CN107011912 A CN 107011912A CN 201710237215 A CN201710237215 A CN 201710237215A CN 107011912 A CN107011912 A CN 107011912A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The present invention relates to pesticide carbendazim microbial inoculum for degrading and preparation method thereof.In agricultural production process, cause agricultural product and agricultural soil agricultural chemicals high residue due to agricultural chemicals is excessively used, be that agricultural products safety and agricultural soil sustainable development bring some problems.The present invention cultivates human pallid bacillus WNPA2 in dedicated liquid fermentation medium, collect zymotic fluid and centrifuge the moisture content removed in zymotic fluid, obtain the thick somatic cells of milky, add glycerine and diatomite, it is uniformly mixed, it is purpose product to obtain white solid preparation.The present invention is a kind of residual treatment technology of environmentally friendly agriculture efficiently, economic, and carbendazim degradation rate can reach more than 50%, has the advantages that processing cost is low, construct simple, non-secondary pollution, effect on environment are small.
Description
Technical field
The invention belongs to geobiont repair technical field, and in particular to a kind of pesticide carbendazim microbial inoculum for degrading and
Its preparation method.
Background technology
Chemical pesticide plays an important role as the important means for ensureing agricultural foison in agricultural production.Chemistry
The subject matter that Pesticide use is present is the unreasonable use of chemical pesticide, Residual Pesticides in Farm Produce severe overweight, cost accounting
Height, severe contamination is caused to ecological environment, generates large area poisoning.Microorganism remediation is the generation using specified microorganisms body
Thank absorption, conversion, remove or degraded chemical pesticide composition, realize the biological control measure that the depollution of environment, ecological effect recover.With height
The features such as imitating economy, non-secondary pollution.The technology is applied to all kinds of pollution fields, shows higher application value, state
It is inside and outside to be considered ecological environmental protection field most worthy and the most treatment technology of vitality about industry department expert, it is agriculture
The main development direction of industry pollution amelioration, is also the effective means for solving chemical pesticide contaminated soil restoration of the ecosystem.
The content of the invention
It is an object of the invention to provide a kind of pesticide carbendazim microbial inoculum for degrading and preparation method thereof, repaiied by biology
Multiple mode is effectively repaired to residues of pesticides soil.
The technical solution adopted in the present invention is：
The preparation method of pesticide carbendazim microbial inoculum for degrading, it is characterised in that：
Comprise the following steps：
Step one：Fermentation：
Liquid fermentation medium A pH is adjusted to 6.5-7.0, and carries out normal sterile processing, by the sterile behaviour of liquid quality 6-9%
Make access human pallid bacillus WNPA2,30 ± 1 DEG C of steady temperature, mixing speed 180-200rpm, ventilation 0.27-0.30M3/
Min, tank pressure 0.07-0.08MPa, continuing fermentation 72-76hr, microscope detection count zymotic fluid in cell number up to 1.5-2.0 ×
109Fermentation is terminated during individual/mL；
Step 2：Somatic cells are separated：
The liquid fermentation medium A of collection step one human pallid bacillus WNPA2 zymotic fluids, are separated with yeast separation centrifuge
Moisture content in zymotic fluid is removed, the thick somatic cells of milky are obtained；
Step 3：Preparation is produced：
In the thick somatic cells of milky that step 2 is obtained, according to thalline：Glycerine volume ratio 1:（ 0.6-1.2）Ratio
Example adds glycerine, and is uniformly mixed, according still further to thalline：Diatomite volume ratio 1：（0.8-1.5）Ratio add fineness be
300 mesh diatomite, are uniformly mixed, and it is purpose product to obtain white solid preparation.
Human pallid bacillus WNPA2 in step one is preserved in Chinese microorganism strain preservation management on November 6th, 2014
Committee's common micro-organisms center, deposit number is CGMCC NO.9943.
The formula of liquid fermentation medium A in step one is as follows：
Glucose 15-20g/L, MgSO4·7H2O 0.5-0.7 g/L, NH4NO31.0-1.5 g/L, (NH4)2SO4 1.5-
2.0 g/L, NaCl 1.0-1.5 g/L, K2HPO4·3H2O 0.5-1.0 g/L, MnSO4·2H2O 0.4-0.6mg/L, surplus
Water is settled to 1L.
Pesticide carbendazim microbial inoculum for degrading made from preparation method as mentioned.
The present invention has advantages below：
The present invention is a kind of environmentally friendly soil restoring technology efficiently, economic, and core technology is the sieve of efficient degrading bacterial strain
Choosing and the preparation of function microbial inoculum, the carbendazim degradation rate for the human pallid bacillus WNPA2 that the present invention is used can reach more than 50%.
The present invention is simple to operate, repairing effect is good, cost is low, the soil restoring technology of agricultural production is not influenceed, compared to other
Method has the advantages that processing cost is low, construct simple, non-secondary pollution, effect on environment are small.To recovering agrological life
State is balanced and productivity has good result.Bioremediation technology can effectively improve agriculture in the application of chemical pesticide pollution control
Field soil environment, simultaneously for protection agricultural product security, promotes the greenization of agricultural product, organises and agricultural land soil is held
Supervention exhibition has good economical, societal benefits.
Embodiment
With reference to embodiment, the present invention will be described in detail.
The preparation method of pesticide carbendazim microbial inoculum for degrading of the present invention, comprises the following steps：
Step one：Fermentation：
Liquid fermentation medium A pH is adjusted to 6.5-7.0, and carries out normal sterile processing, by the sterile behaviour of liquid quality 6-9%
Make access human pallid bacillus WNPA2,30 ± 1 DEG C of steady temperature, mixing speed 180-200rpm, ventilation 0.27-0.30M3/
Min, tank pressure 0.07-0.08MPa, continuing fermentation 72-76hr, microscope detection count zymotic fluid in cell number up to 1.5-2.0 ×
109Fermentation is terminated during individual/mL；
Step 2：Somatic cells are separated：
The liquid fermentation medium A of collection step one human pallid bacillus WNPA2 zymotic fluids, are separated with yeast separation centrifuge
Moisture content in zymotic fluid is removed, the thick somatic cells of milky are obtained；
Step 3：Preparation is produced：
In the thick somatic cells of milky that step 2 is obtained, according to thalline：Glycerine volume ratio 1:（ 0.6-1.2）Ratio
Example adds glycerine, and is uniformly mixed, according still further to thalline：Diatomite volume ratio 1：（0.8-1.5）Ratio add fineness be
300 mesh diatomite, are uniformly mixed, and it is purpose product to obtain white solid preparation.
Human pallid bacillus in step one（Ochrobactrumanthropi）WNPA2 was preserved on November 6th, 2014
China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3, deposit number is CGMCC NO.9943.
The formula of liquid fermentation medium A in step one is as follows：
Glucose 15-20g/L, MgSO4·7H2O 0.5-0.7 g/L, NH4NO31.0-1.5 g/L, (NH4)2SO4 1.5-
2.0 g/L, NaCl 1.0-1.5 g/L, K2HPO4·3H2O 0.5-1.0 g/L, MnSO4·2H2O 0.4-0.6mg/L, surplus
Water is settled to 1L.
Embodiment 1：
Step one：Fermentation：
Liquid fermentation medium A pH is adjusted to 6.0, and carries out normal sterile processing, is accessed by the sterile working of liquid quality 9%
Human pallid bacillus WNPA2,29 DEG C of steady temperature, mixing speed 180rpm, ventilation 0.27M3/ min, tank pressure 0.08MPa, continues
Ferment 74hr, and microscope detection counts in zymotic fluid cell number up to 2.0 × 109Fermentation is terminated during individual/mL；
Liquid fermentation medium A formula is as follows：
Glucose 18g/L, MgSO4·7H2O 0.5 g/L, NH4NO31.0 g/L, (NH4)2SO41.5g/L, NaCl 1.0
G/L, K2HPO4·3H2O 0.5 g/L, MnSO4·2H2O 0.4mg/L, excess water is settled to 1L.
Step 2：Somatic cells are separated：
The liquid fermentation medium A of collection step one human pallid bacillus WNPA2 zymotic fluids, yeast separation centrifuge separation is removed
Moisture content in zymotic fluid is removed, the thick somatic cells of milky are obtained.
Step 3：Preparation is produced：
In the thick somatic cells of milky that step 2 is obtained, according to thalline：Glycerine volume ratio 1:0.8 ratio is added
Glycerine, and be uniformly mixed, according still further to thalline：Diatomite volume ratio 1：It is 300 mesh diatomite that 1 ratio, which adds fineness, is stirred
Mix well mixed, it is purpose product to obtain pale pink solid pharmaceutical preparation.
Embodiment 2：
Step one：Fermentation：
Liquid fermentation medium A pH is adjusted to 6.2, and carries out normal sterile processing, is accessed by the sterile working of liquid quality 6%
Human pallid bacillus WNPA2,31 DEG C of steady temperature, mixing speed 190rpm, ventilation 0.28M3/ min, tank pressure 0.08MPa, continues
Ferment 76hr, and microscope detection counts in zymotic fluid cell number up to 1.8 × 109Fermentation is terminated during individual/mL；
Liquid fermentation medium A formula is as follows：
Glucose 15g/L, MgSO4·7H2O 0.6 g/L, NH4NO31.5 g/L, (NH4)2SO42.0 g/L, NaCl
1.0g/L, K2HPO4·3H2O 0.8g/L, MnSO4·2H2O 0.5mg/L, excess water is settled to 1L.
Step 2：Somatic cells are separated：
The liquid fermentation medium A of collection step one human pallid bacillus WNPA2 zymotic fluids, are separated with yeast separation centrifuge
Moisture content in zymotic fluid is removed, the thick somatic cells of milky are obtained；
Step 3：Preparation is produced：
In the thick somatic cells of milky that step 2 is obtained, according to thalline：Glycerine volume ratio 1:1.2 ratio is added
Glycerine, and be uniformly mixed, according still further to thalline：Diatomite volume ratio 1：It is 300 mesh diatomite that 0.8 ratio, which adds fineness,
It is uniformly mixed, it is purpose product to obtain pale pink solid pharmaceutical preparation.
Embodiment 3：
Step one：Fermentation：
Liquid fermentation medium A pH is adjusted to 6.5, and carries out normal sterile processing, is accessed by the sterile working of liquid quality 8%
Human pallid bacillus WNPA2,30 DEG C of steady temperature, mixing speed 180rpm, ventilation 0.29M3/ min, tank pressure 0.2Mpa, continues
Ferment 72hr, and microscope detection counts in zymotic fluid cell number up to 1.5 × 109Fermentation is terminated during individual/mL；
Liquid fermentation medium A formula is as follows：
Glucose 15g/L, MgSO4·7H2O 0.7 g/L, NH4NO31.5 g/L, (NH4)2SO42.0 g/L, NaCl 1.5
G/L, K2HPO4·3H2O 1.0 g/L, MnSO4·2H2O 0.6mg/L, excess water is settled to 1L.
Step 2：Somatic cells are separated：
The liquid fermentation medium A of collection step one human pallid bacillus WNPA2 zymotic fluids, are separated with yeast separation centrifuge
Moisture content in zymotic fluid is removed, the thick somatic cells of milky are obtained；
Step 3：Preparation is produced：
In the thick somatic cells of milky that step 2 is obtained, according to thalline：Glycerine volume ratio 1:1.1 ratio is added
Glycerine, and be uniformly mixed, according still further to thalline：Diatomite volume ratio 1：It is 300 mesh diatomite that 0.9 ratio, which adds fineness,
It is uniformly mixed, it is purpose product to obtain pale pink solid pharmaceutical preparation.
More than 50% can reach to carbendazim degradation rate using microbial inoculum made from the above method.
Present disclosure is not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention
And any equivalent conversion taken technical solution of the present invention, it is that claim of the invention is covered.
Claims (4)
1. the preparation method of pesticide carbendazim microbial inoculum for degrading, it is characterised in that：
Comprise the following steps：
Step one：Fermentation：
Liquid fermentation medium A pH is adjusted to 6.5-7.0, and carries out normal sterile processing, by the sterile behaviour of liquid quality 6-9%
Make access human pallid bacillus WNPA2,30 ± 1 DEG C of steady temperature, mixing speed 180-200rpm, ventilation 0.27-0.30M3/
Min, tank pressure 0.07-0.08MPa, continuing fermentation 72-76hr, microscope detection count zymotic fluid in cell number up to 1.5-2.0 ×
109Fermentation is terminated during individual/mL；
Step 2：Somatic cells are separated：
The liquid fermentation medium A of collection step one human pallid bacillus WNPA2 zymotic fluids, are separated with yeast separation centrifuge
Moisture content in zymotic fluid is removed, the thick somatic cells of milky are obtained；
Step 3：Preparation is produced：
In the thick somatic cells of milky that step 2 is obtained, according to thalline：Glycerine volume ratio 1:（ 0.6-1.2）Ratio
Example adds glycerine, and is uniformly mixed, according still further to thalline：Diatomite volume ratio 1：（0.8-1.5）Ratio add fineness be
300 mesh diatomite, are uniformly mixed, and it is purpose product to obtain white solid preparation.
2. the preparation method of pesticide carbendazim microbial inoculum for degrading according to claim 1, it is characterised in that：
Human pallid bacillus WNPA2 in step one was preserved in Chinese microorganism strain preservation conservator on November 6th, 2014
Meeting common micro-organisms center, deposit number is CGMCC NO.9943.
3. the preparation method of pesticide carbendazim microbial inoculum for degrading according to claim 1, it is characterised in that：
The formula of liquid fermentation medium A in step one is as follows：
Glucose 15-20g/L, MgSO4·7H2O 0.5-0.7 g/L, NH4NO31.0-1.5 g/L, (NH4)2SO4 1.5-2.0
G/L, NaCl 1.0-1.5 g/L, K2HPO4·3H2O 0.5-1.0 g/L, MnSO4·2H2O 0.4-0.6mg/L, excess water is fixed
Hold to 1L.
4. pesticide carbendazim microbial inoculum for degrading made from preparation method as claimed in claim 1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116083290A (en) * | 2022-11-21 | 2023-05-09 | 陕西省微生物研究所 | Preparation method of compound microbial agent suitable for repairing lead-zinc-antibiotic-resistance gene contaminated soil |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1932005A (en) * | 2006-05-31 | 2007-03-21 | 华东理工大学 | Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme |
| CN103272477A (en) * | 2013-05-29 | 2013-09-04 | 中国科学院生态环境研究中心 | Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof |
| CN104673725A (en) * | 2015-03-05 | 2015-06-03 | 北京师范大学 | Chrysene degradation bacterium and application thereof |
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2017
- 2017-04-12 CN CN201710237215.9A patent/CN107011912B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1932005A (en) * | 2006-05-31 | 2007-03-21 | 华东理工大学 | Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme |
| CN103272477A (en) * | 2013-05-29 | 2013-09-04 | 中国科学院生态环境研究中心 | Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof |
| CN104673725A (en) * | 2015-03-05 | 2015-06-03 | 北京师范大学 | Chrysene degradation bacterium and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| JUCILEUZA CONCEIÇÃO DOS SANTOS1等: "Biodegradation of the fungicide carbendazim by bacteria from Coriandrum sativum L. rhizosphere", 《ACTA SCIENTIARUM. BIOLOGICAL SCIENCES》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116083290A (en) * | 2022-11-21 | 2023-05-09 | 陕西省微生物研究所 | Preparation method of compound microbial agent suitable for repairing lead-zinc-antibiotic-resistance gene contaminated soil |
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