CN1932005A - Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme - Google Patents
Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme Download PDFInfo
- Publication number
- CN1932005A CN1932005A CNA2006100271456A CN200610027145A CN1932005A CN 1932005 A CN1932005 A CN 1932005A CN A2006100271456 A CNA2006100271456 A CN A2006100271456A CN 200610027145 A CN200610027145 A CN 200610027145A CN 1932005 A CN1932005 A CN 1932005A
- Authority
- CN
- China
- Prior art keywords
- stalk
- anthropi
- ibl01
- ochrobactrum anthropi
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.
Description
Technical field
The present invention relates to a kind of human pallid bacillus (Ochrobactrum anthropi), and the application of described bacterial classification in the preparation of degrading plant stalks or important enzyme.
Background technology
Vegetable fibre, as the valuable source of a large amount of natural fiber matter that exist of a class, its regeneration research has been subjected to paying close attention to widely.All there are many shortcomings owing to utilize physics or chemical process to handle bagasse, so many experts have focused on sight on the microbiological treatment.Although can utilize the microorganism of Mierocrystalline cellulose and hemicellulose many, and there are a lot of microorganisms also can utilize xylogen, certain difficulty is all arranged comparatively speaking.As the bacterial classification of the mould patent disclosure of Rui Shi wood and the bacterial classification of viride bibliographical information, its existing remarkable defective is why stalk is difficult under the normal condition and degrades, mainly be because the combination of Mierocrystalline cellulose, hemicellulose and xylogen in the stalk: xylogen combines through the covalent linkage form with hemicellulose, cellulosic molecule is embedded in the centre, makes the enzyme of degraded cellulose be difficult for contacting with cellulosic molecule.The chemical structure of the water-insoluble of xylogen, complexity has also been brought very big difficulty to degraded.So, thorough degraded cellulose, must at first use microorganism to solve the degradation problem of xylogen.The microbe species of lignin degrading is at occurring in nature, can lignin degrading and the microorganism that produces corresponding enzyme only occupy the minority.The degraded fully of xylogen is fungi, bacterium and the coefficient result of corresponding microorganism group normally, and wherein fungi plays main effect.The fungi of lignin degrading mainly contains: white-rot fungi (it is rotten that timber is white in color), brown rot fungus (making timber be brown rot) and soft-rot bacterium, and the above two belong to Basidiomycetes, and soft-rot bacterium belongs to imperfect fungi.The ability of white rot fungus degrading xylogen is better than the ability of its degraded cellulose.
Vegetable fibre, as bagasse is the main byproduct of sugar industry, it is last major portion after the sugarcane mechanical compaction, belong to a kind of in the agricultural solid residue, its composition mainly contains Mierocrystalline cellulose, hemicellulose and xylogen, similar with wood materials, can be used as the raw material that substitutes part timber.Therefore, selecting a kind of bacterial classification, make its effectively degrading plant fiber, reach the purpose of regeneration, is the problem that the parties concerned extremely pay close attention to.
Summary of the invention
The technical issues that need to address of the present invention are to disclose a kind of human pallid bacillus and the application in the preparation of degrading plant stalks or important enzyme thereof, to overcome the above-mentioned defective that prior art exists.
Human pallid bacillus of the present invention (Ochrobactrum anthropi) is to obtain by following method screening the rotten wooden unit on the oriental plant in the East China University of Science campus:---shake-flask culture---is coated with flat board (M9+ bagasse+agar) and---shakes bottle (M9+ bagasse)---being coated with a plate isolation---human pallid bacillus (Ochrobactrumanthropi) in sampling (wooden unit that rots-come at two ball plane trees).
The bacterial classification that obtains on May 10th, 2006, in China's typical culture collection center preservation, preserving number is CCTCC M 206046 Ochrobactrum anthropi IBL01.
Bacterial classification of the present invention has following characteristic:
1. morphological specificity:
(1) form of cell and size: rod-short, 2.0～2.5 * 0.65～0.75 μ m;
(2) formation of spore: do not have;
(3) gramstaining: feminine gender.
2. the growth conditions on various substratum:
(1) the LB agar plate is cultivated (30 ℃, 48 hours)
Colony shape: circular (Circular);
Bacterium colony becomes the mucus shape: flats (Flat), level and smooth (Smooth)
Size: 4～6mm;
Tone: light yellow.
(2) the M9+ bagasse after the modification+agar plate is cultivated (30 ℃, 96 hours)
Colony shape: circular (Circular);
Bacterium colony becomes the mucus shape: flats (Flat), level and smooth (Smooth)
Size: 1.5～2mm;
Tone: oyster white.
3. physiological and biochemical property:
(1) grape sugar assimilation test: the positive
(2) Arabic sugar assimilation test: the positive
(3) sweet dew sugar assimilation test: the positive
(4) N.F,USP MANNITOL assimilation test: the positive
(5) ethanoyl glucosamine assimilation test: the positive
(6) Fructus Hordei Germinatus sugar assimilation test: the positive
(7) oxysuccinic acid assimilation test: the positive
(8) trisodium citrate assimilation test: the positive
(9) capric acid assimilation test: the positive
(10) oxydase: the positive
(11) urea test: the positive
(12) nitrate: feminine gender
(13) tryptophane: feminine gender
(14) amino guanidyl valeric acid: feminine gender
(15) citrate: feminine gender
(16) gelatin: feminine gender
(17) Potassium Gluconate assimilation test: feminine gender
(18) lipid acid assimilation test: feminine gender
(19) toluylic acid assimilation test: feminine gender
(20) 4-nitrophenyl-β maltonic acid test: feminine gender
According to document: Duan Yongxiang.API identification systems and the application in bacteriological analysis thereof, ModernPreventive Medicine, 2004, Vol.31, No.5, the API 20 NE identification systems authentication methods and the above-mentioned test-results that provide show, bacterial classification of the present invention belongs to the human pallid bacillus monoid, but have significantly different with the pale bacillus of protoplast, its main difference part is: original seed substantially all is that hospital obtains in the patient body, and it mainly is the pollution that very easily causes medical instruments, cause the generation infected, the main contaminated wound of this bacterium, organ transplantation, the property inserted operation and the patient who uses antitumor drug and immunosuppressor do not find its effect aspect straw degradative.
Its physio-biochemical characteristics are as follows:
Very easily cause the pollution of medical instruments, cause the generation infected, the main contaminated wound of this bacterium, organ transplantation, insertion operation and the patient who uses antitumor drug and immunosuppressor.The article report is abroad arranged in addition, and it has the ability of producing Hydantoinase, can also divide cynnematin;
Human pallid bacillus of the present invention (Ochrobactrum anthropi) can utilize sucrose, glucose, fructose, filter paper, ethyl cellulose, wood sugar, semi-lactosi, starch, glycerine, agar etc. as the sole carbon source growth, but it can not utilize lactose, glycine, sodium-acetate to grow as sole carbon source.
Adopt the method for human pallid bacillus Ochrobactrum anthropi of the present invention (preserving number CCTCC M206046 Ochrobactrum anthropi IBL01) degrading plant stalks or stalk slag to comprise the steps:
With the single colony inoculation of human pallid bacillus (preserving number CCTCC M 206046 Ochrobactrum anthropiIBL01) in the seed culture medium of the mixture of M9 substratum and stalk or stalk slag, the pH of seed culture medium is 6.5～7.5, in shaking bottle, cultivated 2～6 days under 30～37 ℃, the condition of 180～220rmp, then inoculum is inoculated in the fermention medium of mixture of M9 substratum and stalk or stalk slag, fermentation condition is identical with seed culture, incubation time is 4～6 days, and back cell count can reach 1.4*10
9
In seed culture medium and the fermention medium, the content of stalk or stalk slag is 4～6g/L;
Based on the volume of 1L fermention medium, the inoculum inoculum size is 0.2～0.8ml;
Said stalk comprises cornstalk, straw, straw or sorghum stalk etc., and said stalk cinder ladle is drawn together bagasse, cornstalk slag or wood chip;
Said M9 substratum at component and content at document: Amira A.EI-Gammal, among Zeinatkamel and Zenat Adeeb.Biodegradation of lignocellulosic substances andproduction of sugars and lignin degradation intermediates by four selectedmicrobial strains.Polymer Degradation n and Stability 61 (1998) 535-542. disclosed report has been arranged, the weight percent prescription of seed culture medium of the present invention and fermention medium is as follows:
(1) liquid nutrient medium: Na
2HPO
40.6%, KH
2PO
40.3%, NH
4Cl 0.1%, NaCl0.05%, bagasse 0.5%, surplus is: distilled water.
(2) solid medium: Na
2HPO
40.6%, KH
2PO
40.3%, NH
4Cl 0.1%, NaCl0.05%, bagasse 0.5%, agar 1.5～2.0%, surplus is: distilled water.
According to the preferred scheme of the present invention, stalk or stalk slag are first through peracid treatment, in the hydrochloric acid of weight concentration 10～20%, and dip treating 3 hours, 65～75 ℃ of oven dry are then pulverized, and sieve.
Human pallid bacillus produces cellulase application filter paper method and can record, and human pallid bacillus produces lignin peroxidase Lip and can record with reference to following method:
0.2mol/L tartrate damping fluid (pH3.0) 1.5mL, 15mmol/L veratryl alcohol 1.0mL, crude enzyme liquid 0.4mL, 15mmol/L H
2O
20.1mL start reaction, 25 ℃ of water-bath 3min survey its optical density(OD) in 310nm, replace veratryl alcohol to make blank with the 1.0mL damping fluid, it is an enzyme activity unit (U) that the definition per minute makes the veratryl alcohol of 1 μ mol be oxidized to the required enzyme amount of veratryl aldehyde.Human pallid bacillus produces manganese peroxidase Mnp and can record with reference to following method: 0.11mol/L Sodium.alpha.-hydroxypropionate damping fluid (pH4.5) 3.4mL, 40mmol/LMnSO
40.1mL, crude enzyme liquid 0.4mL, 1.6mmol/L H
2O
20.1ml, starting reaction, 25 ℃ of water-bath 5min survey its optical density(OD) in 240nm, replace MnSO4 to make blank with the 0.1ml damping fluid, definition per minute oxidation 1 μ mol Mn
2+Become Mn
3+, required enzyme amount is an enzyme activity unit (u).
Cellulase in the human pallid bacillus, zytase, lignin peroxidase, the roughly process of the separation of laccase and the thick enzyme of manganese peroxidase and preparation is: the centrifugal supernatant liquor that obtains after the ultrasonication of human pallid bacillus fermented liquid, supernatant liquor is with the albumen of saturated ammonium sulfate precipitation, separate crossing Bio-Gel P30 gel filtration column behind the protein renaturation, DEAE.Sephadex A50 anion-exchange column is crossed at the different peaks that will pass then and the different peaks of wash-out more respectively, CM.Sephadex A50 cationic exchange coloum, different ion columns such as Sephadex G-25 chromatography column are at last through dialysis, concentrate with drying and just can obtain cellulase, zytase, lignin peroxidase, the crude zyme preparation of laccase and manganese peroxidase.
By above-mentioned disclosed technical scheme as seen, bacterial classification of the present invention and employing strain degradation straw of the present invention or stalk slag, has very significant degradation effect, if be used for the microbial host mould or the fungies such as whiterot fungi, brown rot fungus of degraded cellulose or xylogen at present, and these thalline be used for degrading culture cycle of bagasse reaches about about 15 days; We sieve human pallid bacillus be bacterium, its growth cycle is about a relatively short about week, and this human pallid bacillus not only can produce the enzyme of degradation of fibers but also can produce the enzyme of degradation of hemicellulose and the enzyme of lignin degrading.
Description of drawings
Fig. 1 is the growth curve chart of human pallid bacillus in M9+ bagasse (5g/L).
Fig. 2 is the design sketch of thalline degraded cellulose.
Embodiment
Embodiment 1
The fermentation test of human pallid bacillus:
Picking human pallid bacillus list colony inoculation is to the seed culture medium of M9+ bagasse (5g/L) from flat board, the pH of seed culture medium is 6.7, liquid amount is that 50ml/250ml shakes bottle, cultivates four days cell count numbers under 30 ℃, the condition of 200rmp in shaking bottle and can reach 8.0*10
9About.Get in the fermention medium that 0.5ml is inoculated into M9+ bagasse (5g/L), fermentation condition is identical in seed, cultivates that cell count can reach 1.4*10 after five days
10The total amount of the starch, crude protein, soluble sugar and the uronic acid that contain is 102mg/g in the bagasse of our preparation after measured, these can for the small-molecule substance of thalli growth only can on the thalline to 10
9About, this shows that thalline has also utilized macromolecular substance such as lignocellulose.Our thalline surveyed can produce cellulase, hemicellulase and lignoenzyme in addition.
The seed culture medium components by weight percent is as follows:
Na
2HPO
40.6%, KH
2PO
40.3%, NH
4Cl 0.1%, NaCl 0.05%, bagasse 0.5%, surplus are: distilled water.
The components by weight percent and the content of fermention medium are as follows:
Na
2HPO
40.6%, KH
2PO
40.3%, NH
4Cl 0.1%, NaCl 0.05%, bagasse 0.5%, surplus are: distilled water.
The employing filter paper method is tested, and in the fermenting culture, the content that human pallid bacillus produces cellulase is 2000U/L, and the content that human pallid bacillus produces lignin peroxidase Lip is 1000U/L, and its degradation effect is more obvious.
Embodiment 2～4
Human pallid bacillus with the bagasse that boils processing, acid treatment or alkaline purification be sole carbon source " the M9 substratum of modification " and in the comparison test of growing state
---(---40 mesh sieves are crossed in 65 ℃ of oven dry---pulverizing---to volume of water: bagasse=2: 1) boil three times continuously to bagasse 1: bagasse to boil 30min.
In the hydrochloric acid of bagasse 2: bagasse---weight concentration 20%,---40 mesh sieves are crossed in 70 ℃ of oven dry---pulverizing---to dip treating 3 hours.
Bagasse 3: bagasse---among the weight concentration 2%NaOH,---40 mesh sieves are crossed in 70 ℃ of oven dry---pulverizing---to dip treating 2 hours.
Respectively with bagasse 1,2,3 be sole carbon source " " be fermention medium, fermentation condition is with example 1 for the M9 substratum of modification.
Cultivate to count respectively after 1 day, 2 days, 4 days, 6 days and draw as drawing a conclusion:
Count visible bagasse more helps human pallid bacillus than alkaline purification through peracid treatment utilization with blood counting chamber.
Embodiment 5
The cellulase qualitative test
Solid medium: Na
2HPO
40.6%, KH
2PO
40.3%, NH
4Cl 0.1%, NaCl0.05%, Xylo-Mucine 1%, agar 2%, surplus are: distilled water.
Seed culture medium: Na
2HPO
40.6%, KH
2PO
40.3%, NH
4Cl 0.1%, NaCl0.05%, bagasse 0.5%, surplus are: distilled water.
The seed fermentation condition is identical with embodiment 1.Seed fermentation is coated with flat board after getting the suitable gradient of seed liquor 0.1ml dilution two days later, flat board is placed into 37 ℃ the about 1.5mm of incubator cultivation one week back bacterium colony, the congo red staining standby half an hour 1 normal NaCl decolouring of adding 0.1% in flat board the results are shown in Figure 2 in 1. minutes, the obvious transparent circle appears, because so the Congo red flat board that can combine with carboxymethyl cellulose shows red, if thalline can transparent circle will occur around the thalline by degraded cellulose so.
Embodiment 6
Seed culture condition and fermentation culture conditions are with embodiment 1.
The preparation of cellulase: get fermented liquid 20ml ultrasonication 1 hour, the centrifugal 10min of 5000r/min gets supernatant liquor and adds 65% saturated ammonium sulphate albumen, cross Bio-Gel P30 gel column after the centrifugal 10min. protein precipitation of the 5000r/min renaturation, difference according to molecular weight obtains several protein ingredients, measures according to the molecule of cellulase and passes through dialysis, the concentrated and dry crude zyme preparation that just obtains 1.2～1.3mg cellulase again after wherein a kind of component is crossed DEAE.Sephadex A50 anion-exchange column.
The preparation of zytase: fermentation liquor treatment is the same, gets another kind of protein ingredient behind the Bio-Gel P30 gel column and crosses behind DEAE.Sephadex A50 anion-exchange column, the CM.Sephadex A50 cationic exchange coloum through dialysis respectively again, concentrates and the dry crude zyme preparation that just obtains 1.5～1.7mg zytase.
The preparation of lignoenzyme: condition is the same, crossing Bio-Gel P30 gel column, DEAE.Sephadex A50 anion-exchange column, CM.Sephadex A50 cationic exchange coloum and G-25 respectively is Sephadex G-25 chromatography column, the crude zyme preparation of last 2.0～2.3mg lignoenzyme.
Claims (9)
1. a human pallid bacillus (Ochrobactrum anthrop) CCTCC M 206046Ochrobactrum anthropi IBL01.
2. adopt human pallid bacillus (Ochrobactrum anthrop) the CCTCCM 206046 Ochrobactrum anthropi IBL01 described in the claim 1 to can be used for producing and have highly active cellulase, lignoenzyme and hemicellulase.
3. the method for thick enzyme such as separation and Extraction cellulase, zytase, lignin peroxidase, laccase and manganese peroxidase from human pallid bacillus.
4. adopt the method for human pallid bacillus (Ochrobactrum anthropi) CCTCC M 206046Ochrobactrum anthropi IBL01 degrading plant straw, it is characterized in that, comprise the steps: the single colony inoculation of human pallid bacillus CCTCC M 206046 Ochrobactrum anthropi IBL01 is cultivated in the seed culture medium of the mixture of M9 substratum and stalk or stalk slag, then inoculum is inoculated in the fermention medium of mixture of M9 substratum and stalk or stalk slag and cultivates.
5. method according to claim 2 is characterized in that, the pH of seed culture medium and fermention medium is 6.5～7.5.
6 methods according to claim 2 is characterized in that, in seed culture medium and the fermention medium, the content of stalk or stalk slag is 4～6g/L.
7. method according to claim 2 is characterized in that said stalk comprises cornstalk, straw, straw or sorghum stalk, and said stalk cinder ladle is drawn together bagasse, cornstalk slag or wood chip etc.
8. method according to claim 2 is characterized in that, based on the volume of 1L fermention medium, the inoculum inoculum size is 0.2～0.8ml.
9. method according to claim 2 is characterized in that, stalk or stalk slag are first in the hydrochloric acid of weight concentration 10～20%, dip treating 3 hours, and 65～75 ℃ of oven dry are then pulverized, and sieve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100271456A CN1932005A (en) | 2006-05-31 | 2006-05-31 | Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100271456A CN1932005A (en) | 2006-05-31 | 2006-05-31 | Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1932005A true CN1932005A (en) | 2007-03-21 |
Family
ID=37878036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006100271456A Pending CN1932005A (en) | 2006-05-31 | 2006-05-31 | Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1932005A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146424A (en) * | 2010-02-10 | 2011-08-10 | 无锡市金坤生物工程有限公司 | Method for preparing cellulose by degrading straws with complex enzyme |
| CN102417890A (en) * | 2011-12-09 | 2012-04-18 | 江南大学 | Sinorhizobium meliloti and method for applying same for fermenting to produce manganese peroxidase |
| CN101418266B (en) * | 2007-10-23 | 2012-08-29 | 中国科学院福建物质结构研究所 | Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium |
| CN103266099A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 active filler and preparation method thereof |
| CN104630311A (en) * | 2015-02-09 | 2015-05-20 | 江苏联海生物科技有限公司 | Method for synchronously producing straw nano-cellulose and bacterial cellulose by using sweet sorghum |
| CN107011912A (en) * | 2017-04-12 | 2017-08-04 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
| CN111893065A (en) * | 2019-07-30 | 2020-11-06 | 沈阳农业大学 | Low-temperature cellulose degradation bacterium |
| CN113854044A (en) * | 2021-09-25 | 2021-12-31 | 广西壮族自治区环境保护科学研究院 | Process for producing wood-rotting fungus base material by using crop straws |
-
2006
- 2006-05-31 CN CNA2006100271456A patent/CN1932005A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101418266B (en) * | 2007-10-23 | 2012-08-29 | 中国科学院福建物质结构研究所 | Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium |
| CN102146424B (en) * | 2010-02-10 | 2013-03-20 | 无锡市金坤生物工程有限公司 | Method for preparing cellulose by degrading straws with complex enzyme |
| CN102146424A (en) * | 2010-02-10 | 2011-08-10 | 无锡市金坤生物工程有限公司 | Method for preparing cellulose by degrading straws with complex enzyme |
| CN102417890A (en) * | 2011-12-09 | 2012-04-18 | 江南大学 | Sinorhizobium meliloti and method for applying same for fermenting to produce manganese peroxidase |
| CN102417890B (en) * | 2011-12-09 | 2013-01-02 | 江南大学 | Sinorhizobium meliloti and method for applying same for fermenting to produce manganese peroxidase |
| CN103266099B (en) * | 2013-05-29 | 2016-08-24 | 中国科学院生态环境研究中心 | Human pallid bacillus active filler and preparation method thereof |
| CN103266099A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 active filler and preparation method thereof |
| CN104630311A (en) * | 2015-02-09 | 2015-05-20 | 江苏联海生物科技有限公司 | Method for synchronously producing straw nano-cellulose and bacterial cellulose by using sweet sorghum |
| CN107011912A (en) * | 2017-04-12 | 2017-08-04 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
| CN107011912B (en) * | 2017-04-12 | 2019-10-25 | 陕西省微生物研究所 | Pesticide carbendazim microbial inoculum for degrading and preparation method thereof |
| CN111893065A (en) * | 2019-07-30 | 2020-11-06 | 沈阳农业大学 | Low-temperature cellulose degradation bacterium |
| CN111893065B (en) * | 2019-07-30 | 2022-01-25 | 沈阳农业大学 | Low-temperature cellulose degradation bacterium |
| CN113854044A (en) * | 2021-09-25 | 2021-12-31 | 广西壮族自治区环境保护科学研究院 | Process for producing wood-rotting fungus base material by using crop straws |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xie et al. | Biodegradation of ramie stalk by Flammulina velutipes: mushroom production and substrate utilization | |
| CN1932005A (en) | Human ochrobactrum anthropi and its application in degrading plant stalks and preparing important enzyme | |
| CN1769424A (en) | Bacillus strain and its uses | |
| CN107828667A (en) | A kind of myrothecium verrucaria mutant strain T2901 and its application | |
| CN1966670A (en) | Bacillus laterosporus and soil inoculation agent prepared from the strain | |
| CN104762250B (en) | A kind of method using ligno-cellulose hydrolysate production probiotics | |
| CN114672469B (en) | Method for producing laccase by fermenting coarse feed through dzo rumen natural co-culture | |
| CN109251872A (en) | Castoff compost composite bacteria agent, preparation method and castoff compost method | |
| CN103408335B (en) | Microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation | |
| CN103215311A (en) | Method for producing high-quality aquilaria sinensis material through aspergillus niger conversion | |
| CN1185336C (en) | Li's Trichoderma strains and use thereof | |
| CN112725314B (en) | Method for producing endoglucanase by fermenting coarse feed through natural symbiotic mixed culture | |
| CN114164188A (en) | Method for producing laccase by degrading reed stalks with yak rumen anaerobic fungus and methane bacteria co-culture and application | |
| CN105670966B (en) | High-temperature-resistant garden waste decomposing bacteria ST4 and application thereof | |
| CN1888062A (en) | Yeast cell immobilizing method | |
| CN1928074A (en) | Microorganism bacterium agent for degrading lignocellulose and method of degrading lignocellulose using the same | |
| CN103352016A (en) | Method for preparing biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha | |
| CN1229490C (en) | 'Tabin' aspergillus capable of producing pectase in high yield, and application in production of solid-state fermentation | |
| CN113201460B (en) | Medicinal fungus trichoderma brevicompactum and application thereof | |
| CN1948453A (en) | Method of screening laccase endogenic bateria from Chinese sumac and preparing laccase through solid state fermentation | |
| CN105567608B (en) | High-temperature-resistant garden waste decomposing bacteria ST1 and application thereof | |
| CN113475312A (en) | Megapes cracking powder phlobacterium culture medium and culture method thereof | |
| CN113213984B (en) | Biological organic fertilizer containing bacillus vallismortis and preparation method and application thereof | |
| CN1279159C (en) | CZ-81 strain of 'Baibachi' bacterium and artificial culture method | |
| CN1486613A (en) | Batch production process of biological herbicide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |