CN1888062A - Yeast cell immobilizing method - Google Patents
Yeast cell immobilizing method Download PDFInfo
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- CN1888062A CN1888062A CNA2006100992430A CN200610099243A CN1888062A CN 1888062 A CN1888062 A CN 1888062A CN A2006100992430 A CNA2006100992430 A CN A2006100992430A CN 200610099243 A CN200610099243 A CN 200610099243A CN 1888062 A CN1888062 A CN 1888062A
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Abstract
The present invention relates to yeast cell immobilizing method with plant stalk or squeezed residue as carrier. The yeast cell immobilizing method includes the following steps: 1. crushing or squeezing plant stalk to eliminate its water, and sterilizing; 2. inoculating yeast cell to liquid culture medium, mixing the liquid culture medium with yeast cell and the sterilized plant stalk or squeezed residue for solid or semi-solid culturing; and 3. adding multiplying culture medium and further multiplying culture.
Description
Technical field
The present invention relates to a kind of method of fixed yeast cell, in particular to the method for wherein using plant haulm or its remaining slag in squeezing back as the fixed yeast cell of carrier.
Background technology
In alcohol and beer production fermenting process, using fixed yeast is the emerging biotechnology that this century, the seventies was grown up by enzyme immobilization technology.Since the cell density that fixed yeast can make fermentation system at double in addition tens of times of ground increase, and be suitable for operating under the highly diluted rate, thereby can increase substantially fermenting speed, shorten fermentation period, improve equipment capacity and production efficiency, simplifying production process reduces labor intensity, make production process more stable and be easy to control, because above-mentioned advantage, immobilized yeast technology has become the important development direction of alcohol and beer industry fermentation technique, is domestic and international at present very active research field.
Immobilized yeast technology roughly can be divided into absorption method and entrapping method two big classes, the absorption method method is simple, less demanding to reactor, the solid support material physical strength is higher, life-span is longer, thereby cost is lower, but shortcoming be absorption cell concentration very low, when the environmental change of fermented liquid (as high flow rate, CO
2It is fierce to discharge, and it is violent to roll, when fermented liquid PH changes) cell that is adsorbed comes off easily, cause active decline, and easily the assorted bacterium of absorption is difficult to remove [1～6].The advantage of entrapping method is that cell is subjected to the carrier protection to be difficult for running off, and is stronger to the severe environment resist ability, can carry out specialization and produce; but it is complicated that its shortcoming is a making processes, and carrier life is not long, and price is higher; and carrier is easily lost physical strength, blocking pipeline, and make cost improve [7～9].In recent ten years, calcium alginate embedded method is owing to having condition of cure gentleness easy to make, and is stronger to reactor adaptability, is suitable for advantages such as multiplication culture, and is much accounted of use.The weakness that investigators exist according to it has again been invented the proliferative cell method and has been added the method that certain material improves the gel physico-chemical property, and the proliferative cell method is to make CO by the starting point concentration that reduces embedding cell
2The release mitigation that becomes, thereby delay the gel phenomenon of bursting apart.But, and the phosphatic stable of gel obviously do not improved because the starting point concentration of embedding cell is restricted.Improve the method for gel physico-chemical property for adding the power subsidiary material, can make original calcium alginate embedded method obtain very much progress.[10～11] still, all these methods can't fundamentally solve the subject matter that alginate calcium exists.
United States Patent (USP) 5,595 utilizes vegetable material and polymkeric substance to act on the realization cell fixation simultaneously in 893.
Summary of the invention
The stem stalk that the invention provides the use plant is as carrier, according to the various characteristics of himself, not only solved the problems referred to above, also for reduce the fixation support cost, saving that loaded down with trivial details operating process provides may, solve a difficult problem for a long time, make the engineering of immobilized cell be able to widespread use.
According to the present invention, provide and used plant haulm or its to squeeze the method for the remaining slag in back as carrier immobilized yeast cell, this method comprises the following steps:
A) plant haulm is pulverized or further squeezed and remove the moisture that wherein is rich in, sterilization subsequently;
B) yeast cell is inoculated into liquid nutrient medium, the liquid nutrient medium that will contain yeast cell by manipulated variable mixes with plant haulm or its remaining slag in squeezing back of sterilization subsequently, makes the cultivation of yeast cell reach solid-state or the semi-solid state cultivation;
C) add proliferated culture medium subsequently and continue multiplication culture.
Term " crushing juice rate " refers to as used herein, after the squeezing of different plant haulms process, and the volume weight per-cent of the juice of generation and plant haulm.
Fermenting process provided by the invention (being mainly used in alcohol and beer fermentation process) yeast fixation support, it is plant stem with certain deformability, yeast cell wherein is by the embedding of vegetable cell cavity and two kinds of effect onsets simultaneously of plant cell wall absorption, and the immobilization of realization yeast cell.Be the yeast cell that has wrapped up part in the cavity of vegetable cell, the cell walls of vegetable cell relies on electrostatic force to adsorb the part yeast cell simultaneously.This during the fermentation fixed yeast can be used as the provenance of free yeast simultaneously both as the fermentation activity main body.Wherein, the fixation support material is compressible plant haulm, or above-mentioned plant haulm is through the remaining slag in squeezing back, described plant haulm is selected from: sorgo stalk, sugarcane stem, cornstalk, corn cob and reed core, described yeast cell is selected from: yeast saccharomyces cerevisiae, pipe capsule yeast, bread yeast, pichia spp, candiyeast, cordiale yeast, fission yeast and Rhodotorula pallida.The manufacture method of said fixing carrier is that plant haulm is cut into suitable shape and size, and then moisture wherein being squeezed out makes its compression set or directly utilize plant haulm, does not need further drying; It is centrifugal to get seed culture fluid, the gained yeast cell adds in the liquid nutrient medium of the intact bacterium of death of monks or nuns, again it is mixed with plant haulm or its remaining slag in squeezing back of sterilization by manipulated variable, using under the situation of plant haulm as carrier, adding by 4～30 volume/weight % in the weight of plant haulm; Use plant haulm through the situation of the remaining slag of squeezing as carrier under, press the crushing juice rate of plant haulm, in the liquid nutrient medium that contains yeast cell of the weight adding crushing juice rate ± 15 volume/weight % of plant haulm; In fermentor tank, feed sterile air, carry out static state or stir culture, add a certain amount of proliferated culture medium after 4～72 hours and cultivate certain hour, wherein the amount of the proliferated culture medium of Jia Ruing is 80～150 volume/weight % of carrier quality, promptly finishes immobilization process.Glucose concn is 2%～30% (w/v) in the liquid nutrient medium, and glucose concn is 2%～30% (w/v) in the proliferated culture medium.Yeast cell content is 10 in the liquid nutrient medium of inoculation back
4～10
11Individual/milliliter.The yeast cell content that immobilization process finishes in the carrier of back is the dried carrier of 0.1～2g stem cell/g.
Yeast cell immobilization method of the present invention, contain the liquid nutrient medium of yeast cell in adding after, because the control of add-on, liquid nutrient medium all is adsorbed in plant stem inside, make that the yeast cell cultivation of this moment is solid-state or the semi-solid state cultivation, all proliferating cells all are fixed on carrier inside, make the later stage yeast cell of propagation can effectively be fixed in the carrier equally; The advantage of multiplication culture is to have improved the immobilization biological amount, has shortened the production cycle, makes biomass reach stable rapidly simultaneously, makes the fermentation in later stage also can realize rapidly stablizing.
This novel fixation support has solved that carrier in the past is anti-to be washed away and the problem of polishing machine difference, the life-span and the service efficiency of carrier have been improved, solved an important techniques difficult problem for produce fields such as alcohol fuel, alcohol drink at biological fermentation, according to the present invention, this method is preferably used for producing ethanol; Simultaneously, such carrier is cheap; Process for fixation is very simple, and is workable.
The present invention's characteristics compared with prior art have five aspects:
1, in the process for fixation operating process, existing method is finished usually in two steps, i.e. the preparation of fixation support before this is carrier then and the combining of cell, and each step is trouble all, complex operation.And used carrier is need therefore not save the step of preparing carriers through the plant haulm (as sweet sorghum slag, bagasse) of any processing among the present invention; The mortise of carrier and cell is the natural result after both interact, and need not artificial operation and control, and is very effectively simple.
2, simple adsorption is not only arranged among the present invention between carrier and the cell, main is that carrier is that vegetable cell has a kind of embedding effect to yeast cell, in other words be exactly this process for fixation neither simple absorption neither be simple embedding, but the combination of embedding and absorption.Simultaneously, the advantage of making embedded material with plant haulm is rigid condition gentleness, nontoxic, good biocompatibility.
3, during the fermentation, fermented liquid can pass in and out carrier, and the fermentative action of viable yeast can be given full play to from the carrier surface to inside.Since plant stem absorbs moisture expand after, self have fine plasticity and stability, and between itself and the cell by embedding effect and certain electrostatic force, make cell that stronger shock resistance, polishing machine be arranged.The protection cell does not run off because of washing away, rubbing.In use, can avoid the deadly defect of calcium alginate gel, promptly because of CO
2Discharge fierceness and disintegration and lose physical strength because of the phosphoric acid salt unstable.Can avoid the weak shortcoming of the not high anti-assorted bacterium ability of adsorption rate of simple absorption method, can avoid the simple short shortcoming of gel embedding method carrier life again, and show exclusive advantage.
4, in addition,, can select the carrier of different quantities and different shapes size, in advance with material plant cane cutting processing or cutting processing is not difficult again after making carrier for the needs of different fermentations technology.
5, last, the used novel carriers plant haulm of the present invention (sweet sorghum slag, bagasse, corn stalk etc.) all is a trade waste, need not cost, and the source is abundant.
Description of drawings
Fig. 1 is illustrated in the observed yeast cell figure that is embedded in sorgo stalk inside under the scanning electron microscope, and magnification is 200,000 among Fig. 1 a, and magnification is 100,000 among Fig. 1 b.
Embodiment
Below specify all respects of the present invention and feature by preferred embodiment.It should be appreciated by those skilled in the art that these embodiment just are used for illustration purpose, and do not limit the scope of the invention.Protection scope of the present invention only is subjected to the restriction of claims.Under the condition that does not deviate from claims scope.Those skilled in the art can carry out various modifications and improvement to various aspects of the present invention, and these modifications and improvement also belong to protection scope of the present invention.
In addition, unless it should be noted that and specialize, below among the embodiment used various materials and reagent all be material and reagent commonly used in this area, can obtain by conventional commercial sources; Method therefor is and well known to a person skilled in the art ordinary method.
Embodiment 1. is that carrier immobilized yeast fermentation is produced alcohol with the sweet sorghum slag
One, material:
1 bacterial classification: yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) is available from market, Beijing
2 sorgo stalks: take from Hebei
3 substratum:
(1) test tube slant solid medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, agar 20g/L, pH 3.5～7.
(2) liquid nutrient medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, pH 3.5～7.
(3) proliferated culture medium: glucose 150g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L, pH 3.5～7.
(4) fermention medium: glucose 50g/L, sucrose 100g/L, fructose 50g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
4Lg/L.
(5) plant and instrument: electric furnace, constant incubator, shaking table, microscope.
Two, method
1, the preparation of mother liquor: bacterium liquid is cultivated: get one of test tube slant, inserts 250 milliliters of triangular flasks (including the liquid nutrient medium of 100ml), 30 ℃, 200rpm rotational oscillation are cultivated 24 hours promptly maturation are standby.The yeast growth situation of taking a sample to check, no living contaminants can use.Get the some milliliters of seed liquor, centrifugal under the aseptic condition, add the stroke-physiological saline solution dilution after the removal supernatant liquor, stand-by.
2, fixed yeast preparation:
(1) sorgo stalk preparing carriers: with the leaf and the manual removal of skin of sorgo stalk, then stalk is pulverized earlier, granular size is 5 * 5 * 5mm.With the homemade squeezer in laboratory the squeezing of the sorghum stalk of powder essence is obtained sweet sorghum slag and sorgo juice.Crushing juice rate is by 50%.
(2) yeast immobilization: the sweet sorghum slag 25g that obtains after pressure is pressed, in the triangular flask of packing into 121 ℃, sterilization 20min.Above-mentioned mother liquor is sneaked in 8% ratio in the liquid nutrient medium of 25ml, again the gone out sweet sorghum slag of bacterium of liquid nutrient medium and 25g is mixed, cultivate 24h.Change fresh proliferated culture medium 50ml twice, cultivate and reached maximum biomass in the carrier in two days.Fixed yeast is applicable to various reactors and different processing requirements, carries out zymotechnique.
Embodiment 2. is that carrier immobilized yeast fermentation is produced alcohol with sorgo stalk
One, material:
1 bacterial classification: yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) is available from market, Beijing
2 sorgo stalks: take from Hebei
3 substratum:
(1) test tube slant solid medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, agar 20g/L, pH 3.5～7.
(2) liquid nutrient medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, pH 3.5～7.
(3) proliferated culture medium: glucose 150g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L, pH 3.5～7.
(4) fermention medium: glucose 50g/L, sucrose 100g/L, fructose 50g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L.
(5) plant and instrument: electric furnace, constant incubator, shaking table, microscope.
Two, method
1, the preparation of mother liquor: bacterium liquid is cultivated: get one of test tube slant, inserts 250 milliliters of triangular flasks (including the liquid nutrient medium of 100ml), 30 ℃, 200rpm rotational oscillation are cultivated 24 hours promptly maturation are standby.The yeast growth situation of taking a sample to check, no living contaminants can use.Get the some milliliters of seed liquor, centrifugal under the aseptic condition, add the stroke-physiological saline solution dilution after the removal supernatant liquor, stand-by.
2, fixed yeast preparation:
(1) sorgo stalk preparing carriers: with the leaf and the manual removal of skin of sorgo stalk, then stalk is pulverized earlier, granular size is 10 * 10 * 10mm.
(2) yeast immobilization: get sorgo stalk 50g, in the triangular flask of packing into 121 ℃, sterilization 20min.Above-mentioned mother liquor is sneaked in 20% ratio in the liquid nutrient medium of 10ml, again the gone out sorgo stalk of bacterium of liquid nutrient medium and 50g is mixed, cultivate 24h.Change fresh proliferated culture medium 50ml twice, cultivate and reached maximum biomass in the carrier in two days.Fixed yeast is applicable to various reactors and different processing requirements, carries out zymotechnique.
Embodiment 3. is that carrier immobilized yeast fermentation is produced alcohol with bagasse
One, material:
1 bacterial classification: pipe capsule yeast (Pachysolen tannophilus) microbial food fermentation institute
2 purple skin sugarcanes: available from Guangxi
3 substratum:
(1) test tube slant solid medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, agar 20g/L, pH 3.5～7.
(2) liquid nutrient medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, pH 3.5～7.
(3) proliferated culture medium: glucose 150g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L, pH 3.5～7.
(4) fermention medium: glucose 50g/L, sucrose 100g/L, fructose 50g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L.
(5) plant and instrument: electric furnace, constant incubator, shaking table, microscope.
Two, method
1, the preparation of mother liquor: bacterium liquid is cultivated: get one of test tube slant, inserts 250 milliliters of triangular flasks (including the liquid nutrient medium of 100ml), 30 ℃, 200rpm rotational oscillation are cultivated 24 hours promptly maturation are standby.The yeast growth situation of taking a sample to check, no living contaminants can use.Get the some milliliters of seed liquor, centrifugal under the aseptic condition, add the stroke-physiological saline solution dilution after the removal supernatant liquor, stand-by.
2, fixed yeast preparation:
(1) sugarcane preparing carriers: with leaf and the manual removal of skin of sugarcane, then stalk is pulverized earlier, granular size is 20 * 20 * 20mm.With the homemade squeezer in laboratory the cane milling of powder essence is obtained bagasse and sugar cane juice.Crushing juice rate is by 50%.
(2) yeast immobilization: the bagasse 25g that obtains after pressure is pressed, in the triangular flask of packing into 121 ℃, sterilization 20min.Above-mentioned mother liquor is sneaked in 15% ratio in the liquid nutrient medium of 30ml, again the gone out sweet sorghum slag of bacterium of liquid nutrient medium and 25g is mixed, cultivate 24h.Change fresh proliferated culture medium 50ml twice, cultivate and reached maximum biomass in the carrier in two days.Fixed yeast is applicable to various reactors and different processing requirements, carries out zymotechnique.
Embodiment 4. is that carrier immobilized yeast fermentation is produced alcohol with corn cob
One, material:
1 bacterial classification: bread yeast (baker yeast) is available from market, Beijing
2 corn cobs: available from Hebei
3 substratum:
(1) test tube slant solid medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, agar 20g/L, pH 3.5～7.
(2) liquid nutrient medium: sugared 20g/L, yeast powder 3g/L, peptone 5g/L, wort 3g/L, pH 3.5～7.
(3) proliferated culture medium: glucose 150g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L, pH 3.5～7.
(4) fermention medium: glucose 50g/L, sucrose 100g/L, fructose 50g/L, KH
2PO
40.5g/L, peptone 5g/L, MgSO
41g/L.
(5) plant and instrument: electric furnace, constant incubator, shaking table, microscope.
Two, method
1, the preparation of mother liquor: bacterium liquid is cultivated: get one of test tube slant, inserts 250 milliliters of triangular flasks (including the liquid nutrient medium of 100ml), 30 ℃, 200rpm rotational oscillation are cultivated 24 hours promptly maturation are standby.The yeast growth situation of taking a sample to check, no living contaminants can use.Get the some milliliters of seed liquor, centrifugal under the aseptic condition, add the stroke-physiological saline solution dilution after the removal supernatant liquor, stand-by.
2, fixed yeast preparation:
(1) corn core carrier preparation: with the manual removal of maize grain peels, then corn cob is pulverized earlier, granular size is 30 * 30 * 30mm.With the homemade squeezer in laboratory the squeezing of the corn cob of powder essence is obtained the corn cob slag.Crushing juice rate is by 50%.
(2) yeast immobilization: the corn cob slag 25g that obtains after pressure is pressed, in the triangular flask of packing into 121 ℃, sterilization 20min.Above-mentioned mother liquor is sneaked in 8% ratio in the liquid nutrient medium base of 25ml, again the gone out corn cob slag of bacterium of liquid nutrient medium and 25g is mixed, cultivate 24h.Change fresh proliferated culture medium 50ml once, cultivate and reached maximum biomass in the carrier in one day.Fixed yeast is applicable to various reactors and different processing requirements, carries out zymotechnique.
Reference:
[1]Minier，M.，and?Goma，G.Biotechnol.Bioeng.，1981，24，781.
[2]Scott，C.D.，Hancher，C.W.，and?Arcuri，E.J.Adv.Biotechnol.，1981，1，651.
[3]Dauglis，A.J.，Brown，N.H.，Cluett，W.R.，and?Dunlop，D.B.Biotechnol.Lett.，1981，3，651.
[4]Arcuri，E.J，Biotechnol.Bioeng.，1982，24，595.
[5]Del?Borghi，M.，Converti，A.，Parisi；E.，and?Pamment，N.B.Appl.Microbiol.Biotechnol.，1986，23，413.
[6]Vega，J.L.，O’Malley，J.D.，Clausen，E.C.，and?Gaddy，J.L.Biotechnol?Bioeng.Symp.15，1985，263.
[7]Siess，M.H，and?Divies，C.Adv.Appl.Microbiol.，1981，22，1.
[8]Fukushima，S.，and?Hanai，S.in?Chibata，I.，Fukui，S.，and?Wingard，L.S.Jr.’Enzyme?Engineering’.1982，6，347.
[9]Fukushima，S.，and?Yamade，K.in‘Advances?in?fermentation?1983’.Conferenceheld?at?Chelsea?College?London，September，1983，p.10.(Published?by?ProcessBiochemistry).
[10]Birnbaum，S.，Pendleton，R.，Larsson，P.O.，and?Mosbach，K.Biotechnol.Lett.，1981，3，393.
[11]Wada，M.，Kato，J.，and?Chibata，I.Eur.J.Appl.Microbiol.Biotechnol.，1981，11，67.
Claims (10)
1. the method for a fixed yeast cell wherein uses plant haulm or its remaining slag in squeezing back as carrier, and this method comprises the following steps:
A) plant haulm is pulverized or further squeezed and remove the moisture that wherein is rich in, sterilization subsequently;
B) yeast cell is inoculated into liquid nutrient medium, the liquid nutrient medium that will contain yeast cell by manipulated variable mixes with plant haulm or its remaining slag in squeezing back of sterilization subsequently, makes the cultivation of yeast cell reach solid-state or the semi-solid state cultivation;
C) add proliferated culture medium subsequently and continue multiplication culture.
2. according to the process of claim 1 wherein that described plant haulm is selected from: sorgo stalk, sugarcane stem, cornstalk, corn cob and reed core.
3. according to the process of claim 1 wherein that the size of described plant haulm is 0.008～30cm
3/ piece.
4. according to the method for claim 3, the size of wherein said plant haulm is 0.1～8cm
3/ piece.
5. according to the process of claim 1 wherein that described yeast cell is selected from: yeast saccharomyces cerevisiae, pipe capsule yeast, bread yeast, pichia spp, candiyeast, cordiale yeast, fission yeast and Rhodotorula pallida.
6. needs are further dry in step a) according to the process of claim 1 wherein described plant haulm.
7. according to the process of claim 1 wherein in described step b), yeast cell content is 10 in the liquid nutrient medium of inoculation back
4～10
11Individual/milliliter.
8. according to the method for claim 1, the liquid nutrient medium that wherein in described step b), contains yeast cell by the plant haulm of manipulated variable and sterilization or its remaining slag blended in squeezing back, using under the situation of plant haulm as carrier, weight in plant haulm adds by 4～30 volume/weight %, preferably adds by 4～20 volume/weight %; Use plant haulm through the situation of the remaining slag of squeezing as carrier under, press the crushing juice rate of plant haulm, in the liquid nutrient medium that contains yeast cell of the weight adding crushing juice rate ± 15 volume/weight % of plant haulm.
9. according to the process of claim 1 wherein that the amount of the proliferated culture medium that adds is 80～150 volume/weight % of carrier quality in described step c).
10. according to the process of claim 1 wherein that immobilization process finishes that yeast cell content is up to the dried carrier of 2g stem cell/g in the carrier of back.
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|---|---|---|---|
| CNA2006100992430A CN1888062A (en) | 2006-07-21 | 2006-07-21 | Yeast cell immobilizing method |
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|---|---|---|---|
| CNA2006100992430A CN1888062A (en) | 2006-07-21 | 2006-07-21 | Yeast cell immobilizing method |
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|---|---|
| CN1888062A true CN1888062A (en) | 2007-01-03 |
Family
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845462A (en) * | 2010-05-27 | 2010-09-29 | 山西金绿禾燕麦研究所 | Method for producing alcohol by using stalks of jerusalem artichoke as raw material and adopting immobilized yeast fermentation of inulin |
| CN102851320A (en) * | 2012-08-24 | 2013-01-02 | 太仓市周氏化学品有限公司 | Fermentation method for producing ethanol by immobilized yeast |
| CN103642783A (en) * | 2013-12-06 | 2014-03-19 | 江苏科技大学 | Edible carrier immobilized yeast as well as preparation method and application thereof |
| CN103725620A (en) * | 2014-01-03 | 2014-04-16 | 湖南大学 | Activated sludge yeast for treating high-concentration decentralized domestic sewage as well as preparation method and application of activated sludge yeast |
| WO2014089812A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Method for producing ethanol by coupling immobilization bed fermentation with separation |
| WO2014089811A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Preparation method of yeast cell immobilization medium and application thereof |
| CN105255652A (en) * | 2015-11-17 | 2016-01-20 | 湖北工业大学 | Method for producing sauce-flavored white liquor through immobilized liquid fermentation |
-
2006
- 2006-07-21 CN CNA2006100992430A patent/CN1888062A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845462A (en) * | 2010-05-27 | 2010-09-29 | 山西金绿禾燕麦研究所 | Method for producing alcohol by using stalks of jerusalem artichoke as raw material and adopting immobilized yeast fermentation of inulin |
| CN102851320A (en) * | 2012-08-24 | 2013-01-02 | 太仓市周氏化学品有限公司 | Fermentation method for producing ethanol by immobilized yeast |
| WO2014089812A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Method for producing ethanol by coupling immobilization bed fermentation with separation |
| WO2014089811A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Preparation method of yeast cell immobilization medium and application thereof |
| CN103642783A (en) * | 2013-12-06 | 2014-03-19 | 江苏科技大学 | Edible carrier immobilized yeast as well as preparation method and application thereof |
| CN103642783B (en) * | 2013-12-06 | 2015-12-09 | 江苏科技大学 | A kind of edible carrier immobilized yeast and its preparation method and application |
| CN103725620A (en) * | 2014-01-03 | 2014-04-16 | 湖南大学 | Activated sludge yeast for treating high-concentration decentralized domestic sewage as well as preparation method and application of activated sludge yeast |
| CN105255652A (en) * | 2015-11-17 | 2016-01-20 | 湖北工业大学 | Method for producing sauce-flavored white liquor through immobilized liquid fermentation |
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