CN103691305A - Preparation method of Thermus-containing active filler - Google Patents
Preparation method of Thermus-containing active filler Download PDFInfo
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- CN103691305A CN103691305A CN201310697441.7A CN201310697441A CN103691305A CN 103691305 A CN103691305 A CN 103691305A CN 201310697441 A CN201310697441 A CN 201310697441A CN 103691305 A CN103691305 A CN 103691305A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a preparation method of a Thermus-containing active filler. The Thermus scotoductus ZDL1 is an isolate from a medium/high-temperature bioreactor for treating sulfur dioxide and other sulfur-containing waste gases in a water pollution control laboratory at Research Center for Eco-Environmental Sciences of Chinese Academy of Sciences; and the strain is collected to China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7430. The Thermus-containing active filler has the advantages of large specific area, high porosity, favorable air permeability, small resistance, high bacterial cell loading capacity and low tendency to loss; by loading the dominant bacterium Thermus ZDL1, the Thermus-containing active filler has higher efficiency for degrading sulfur dioxide, carbon bisulfide and other sulfur-containing malodorous substances; and since the adaptive phase of the dominant bacterium is short, the degradation reaction can be started quickly, and thus, the Thermus-containing active filler can shorten the starting period when being used in a bioreactor for purifying medium/high-temperature sulfur-containing malodorous substances.
Description
Technical field:
The invention belongs to environmental improvement field, be specifically related to a kind of preparation method of the active filler that contains the hot bacterium that dwells.
Background technology:
In the processes such as sewage disposal, sludge anhydration burning, compost, can produce the waste gas of temperature higher (35～100 ℃), this medium/high exhaust gas constituents is complicated, and wherein sulfur dioxide is one of main material.The waste gas that contains sulfur dioxide spills in atmosphere, along with the diffuses flow of air is to everywhere, surrounding environment is polluted, and health is produced to harm.At present, the improvement of waste gas causes that people more and more pay close attention to, and many countries have all formulated relevant discharge standard, strictly control the discharge of pollutant in waste gas, and require enterprise before discharge, take corresponding control measures.In the national < < discharge standard of air pollutants > > (GB16297-1996) that China came into effect in calendar year 2001, to 33 kinds of atmosphere pollutions, such as the emission limit of the pollutants such as sulfur dioxide, made strict regulation.
Compare with other physical chemistry waste gas processing method, bioremediation has the advantages such as cost is low, easy and simple to handle, technology clean, non-secondary pollution because of it, and the nineties becomes the focus of current research later.Biological ratio juris is to utilize the biological oxidation of microorganism sulphur-containing substance in waste gas to be converted into the process of sulfate or sulphur simple substance.Biological method is suitable for processing the waste gas of low concentration, atm number.
But at present conventional biological treatment technology for waste gas is all the material of processing under normal temperature, the method for processing again after conventionally adopting spraying cooling water to waste gas cooling to the processing of high-temp waste gas.Like this, not only increase energy consumption, and needed to process the new a large amount of waste water that produce, strengthened processing cost.
Therefore, searching can adapt to the hot bacterium that dwells of medium/high environment existence, utilizes the hot bacterium centering/high-temp waste gas of dwelling directly to process, and without cooling system, thereby when reaching exhaust-gas treatment requirement, simplifies treatment process, reduces costs.
In addition, in prior art, for the treatment of all filling a certain amount of carrier in the bioreactor of waste gas, attached growth of microorganisms is processed waste gas at carrier surface.So suitable carrier should have certain surface nature, be applicable to adhering to and growing of microorganism.When sulfurous gas that the waste gas of processing is medium/high, used carrier also needs to have high temperature resistant and corrosion resistant character.Conventional exhaust-gas treatment bioreactor adopts the organic matters such as composting material, wood chip, bark as carrier, and this class organic carrier specific area is little, and heatproof, not perishable, and the pressure loss is large, and service life is short; And the inert carrier surfaces such as volcanic rock, haydite, active carbon have loose structure, specific area is large, and can tolerate higher temperature.
Summary of the invention:
The problem to be solved in the present invention is to provide a kind ofly can process the dwell preparation method of active filler of hot bacterium of containing of medium/high waste gas.
Concrete preparation process is as follows:
(1) inclined-plane is cultivated: cultivate and prepare the hot bacterium slant strains of dwelling;
The described hot bacterium slant medium of dwelling consists of: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, agar 15g, pH value 6.0-7.5,121 ℃ of sterilizing 30min.
(2) preparation of bacteria suspension: the hot bacterium inclined-plane thalline of dwelling of respectively being prepared by step (1) is by 5-20% inoculum concentration fermented and cultured step by step, the separated cell concentration that obtains is the 0.4-0.8g/L hot bacterium bacteria suspension of dwelling;
The hot bacterium collecting cells method of dwelling is as follows;
The hot bacterium inclined-plane thalline of dwelling prepared by step (1) is by 5-20% inoculum concentration fermented and cultured step by step, cultivation temperature 55-60 ℃ of incubation time 36-48h, ventilation 5-200L/min, dissolved oxygen 30%, tank pressure 0.03-0.10Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 2000-8000rpm, centrifugal concentrating 5-20min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.4-0.8g/L hot bacterium bacteria suspension of dwelling;
Consisting of of the described hot bacteria liquid fermentation medium of dwelling: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5,121 ℃ of sterilizing 30min;
Described hot bacterium the consisting of without bacterial nutrient solution of dwelling: powdered beef 3.0-5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5,121 ℃ of sterilizing 30min;
(3) preparation of microbial activity filler:
(a) the microbial bacteria suspension of being prepared by step (2) sprays the surface of porous material; (b) the corrosion-resistant and high-temperature resistant porous material piece (or particle) with the hot bacterium bacterial cell of dwelling being put into impeller rotation mixes; (c) again to corrosion-resistant and high-temperature resistant porous material surface spray microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
Preferred repeating step (a)-(c) 2-3 time, makes equal appendix in material surface and micropore have bacterial cell, obtains microbial activity filler.
Described porous material is one or more in haydite, granular activated carbon, volcanic rock, sponge;
Described porous material density is 0.7-1.0kg/m
3, porosity is 50-96%, particle diameter is 5-50mm;
The described hot bacterium that dwells is specially water pipe blackening hot bacterium (Thermus Scotoductus) ZDL1 of dwelling, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 8th, 2013, deposit number is CGMCC No.7430, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101;
This bacterial strain feature is as follows:
This bacterial strain colony colour on LB solid plate is white, opaque, out-of-shape, and the smooth of the edge, moistening.Thalline is elongated rod shape, size is 0.5 – 1.5 * 2.5 – 15.0 μ m, atrichia and spore, Gram-negative, catalase and oxidase positive, can utilize nitrate, responsive to penicillin, erythromycin, tetracycline, chloramphenicol and streptomysin, but there is anti-PB and rifampin characteristic.Optimum growth temperature is 30-100 ℃;
The filler of being prepared by this bacterium is 0.001-200m at volume
3, the total concentration of sulfide is 30-250ppm, gas flow rate is 0.05-10000m
3/ h, purification temperature is 50-65 ℃, under time of staying 15s-3min condition, the degradation efficiency of sulfide is reached more than 85%.
Described water pipe blackening dwell hot bacterium ZDL1 with the form appendix of bacteria suspension on filler;
Dwell hot bacterium ZDL1 bacteria suspension and mass ratio corrosion-resistant, high temperature resistant porous material of described water pipe blackening is 50-300: 10;
In the described hot bacterium active filler of dwelling, the dwell cell concentration of hot bacterium ZDL1 of water pipe blackening is greater than 10
6cFU/g
(active filler).
Odorous gas processing is carried out in the described hot bacterium active filler of dwelling, particularly at degraded SO
2application in gas, concrete steps are as follows:
(1) appendix there is is the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 1-30 days; Activation temperature 45-55 ℃.
Described SO
2concentration be 50-200ppm;
(2) the microbial activity filler after activation is placed in to bioreactor, passes into the gas of sulphur-containing substance, the water pipe blackening sulphur-containing substance in hot bacterium ZDL1 purifying exhaust air of dwelling, microbial activity packing volume is 0.001-200m
3, the total concentration of sulfide is 30-250ppm, gas flow rate is 0.05-10000m
3/ h, purification temperature is 50-65 ℃; Regularly to microbial activity filling surface spraying nutrient solution, maintain the activity of microbial activity filler, spray frequency is to spray 1 time for 1-5 days, and each spray rate is 1.0-200L/min, and each spray time is 20-120min;
Consisting of of described nutrient solution: powdered beef 3.0-5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5; Follow the tracks of the degradation capability that detects sulfur-containing foul material.
The present invention adopts gas chromatography to follow the tracks of and detects sulfur-containing compound; Described gas chromatography is: adopt the analysis of Agilent GC-6890N gas chromatograph to measure sulfide concentration, chromatographic column is DB-1701 capillary column (30m * 0.32mm * 0.25 μ m), vaporizer, FPD detector, column temperature are set to respectively 100 ℃, 200 ℃, 50 ℃, sample size 100 μ L, N
2flow velocity is 1.7mL/min, and shunt volume is 34.6mL/min.
Beneficial effect:
1. the hot bacterium active filler appendix of the dwelling of the present invention bacterium water pipe blackening hot bacterium ZDL1 of dwelling that has superiority, has the efficiency of the sulfur-containing foul materials such as higher degraded sulfur dioxide, carbon disulfide; And the laundering period of dominant bacteria is short, can start fast degradation reaction, be applied in the waste gas bioreactor that purifies medium/high sulfur-containing foul material, can shorten the starting period.
2. utilize sedimentation, adhere to the effect with the multiple mechanics such as centrifugal, the blackening of superior strain water pipe is dwelt to hot bacterium ZDL1 appendix on corrosion-resistant and high-temperature resistant porous material, form microbial activity filler, bacterial cell not only can be attached to filling surface equably, and can enter in the hole of filler inside, make the bacterial cell appendix amount of microbial activity filler high, be difficult for running off from bioreactor.
3. the hot bacterium active filler of dwelling is used front through overactivation, can make bioreactor start fast.The bacterial cell enzyme of appendix is lived stable, can maintain the clean-up effect of sulphur-containing exhaust gas steady in a long-term.
4. for rough surface, the porous of the carrier material of microbial activity filler, be suitable for bacterial cell and adhere to; Specific area is large, good permeability, and resistance is little, is conducive to bacterial cell and contacts with the sulphur-containing substance in waste gas, improves mass-transfer efficiency.
5. filler used in the present invention is high temperature resistant, corrosion-resistant, is applied in the bioreactor that purifies medium/high sulphur-containing exhaust gas, and the pressure loss is little, and the physical condition of Various Complex can be used and adapt to not perishable distortion for a long time.
6. the prepared active filler of the present invention is 30-250ppm in the total concentration of sulfide, and gas flow rate is 0.05-10000m
3/ h, purification temperature is 50-65 ℃, under time of staying 15s-3min condition, the degradation efficiency of sulfide is reached more than 85%.
The specific embodiment:
Embodiment 1: a kind of preparation method who removes the hot bacterium active filler of dwelling of sulphur-containing substance purification medium/high sulphur-containing exhaust gas
(1) inclined-plane is cultivated: cultivate and prepare the water pipe blackening hot bacterium ZDL1 slant strains of dwelling;
Slant medium consists of: powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 ℃ of sterilizing 30min.
(2) preparation of bacteria suspension: the water pipe blackening of respectively being prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, the separated cell concentration that obtains is the 0.6g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
The water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, 60 ℃ of incubation time 48h of cultivation temperature, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.6g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
Liquid fermentation medium consist of powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
Without consisting of of bacterial nutrient solution: powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
(3) preparation of microbial activity filler:
(a) it is 200kg/m that the microbial bacteria suspension of being prepared by step (2) sprays density
3, porosity is 52%, and particle diameter is 200mm volcanic rock particle surface, and the ratio of bacteria suspension and volcanic rock particle is 100:80; (b) the volcanic rock grain particle of the hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening being put into impeller rotation mixes; (c) again to volcanic rock grain particle surface spray microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
Repeating step (a)-(c) 3 time, makes equal appendix in volcanic rock grain particle surface and micropore have bacterial cell, obtains microbial activity filler.
The water pipe blackening cell concentration 5.0 * 10 of hot bacterium ZDL1 of dwelling in described microbial activity filler
7cFU/g
(volcanic rock particle).
Embodiment 2: a kind of dwell preparation method of hot bacterium active filler of water pipe blackening that sulphur-containing substance purifies medium/high sulphur-containing exhaust gas that removes
(1) inclined-plane is cultivated: cultivate and prepare the water pipe blackening hot bacterium ZDL1 slant strains of dwelling;
Slant medium consists of: powdered beef 4.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 ℃ of sterilizing 30min.
(2) preparation of bacteria suspension: the water pipe blackening of respectively being prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, the separated cell concentration that obtains is the 0.6g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
The water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, 60 ℃ of incubation time 48h of cultivation temperature, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.6g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
Liquid fermentation medium consist of powdered beef 4.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
Without consisting of of bacterial nutrient solution: powdered beef 4.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
(3) preparation of microbial activity filler:
(a) it is 1.0kg/m that the microbial bacteria suspension of being prepared by step (2) sprays density
3, porosity is 85%, and particle diameter is 20mm seed activity carbon surface, and the ratio of bacteria suspension and granular activated carbon is 100:15; (b) granular activated carbon of the hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening being put into impeller rotation mixes; (c) again to granular activated carbon surface spraying microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
Preferred repeating step (a)-(c) 3 time, makes equal appendix in seed activity carbon surface and micropore have bacterial cell, obtains microbial activity filler.
The water pipe blackening cell concentration 5.0 * 10 of hot bacterium ZDL1 of dwelling in described microbial activity filler
8cFU/g
(granular activated carbon).
Embodiment 3: a kind of preparation method who removes the hot bacterium active filler of dwelling of sulphur-containing substance purification medium/high sulphur-containing exhaust gas
(1) inclined-plane is cultivated: cultivate and prepare the water pipe blackening hot bacterium ZDL1 slant strains of dwelling;
Slant medium consists of: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 ℃ of sterilizing 30min.
(2) preparation of bacteria suspension: the water pipe blackening of respectively being prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, the separated cell concentration that obtains is the 0.6g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
The water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, 60 ℃ of incubation time 48h of cultivation temperature, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.6g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
Liquid fermentation medium consist of powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
Without consisting of of bacterial nutrient solution: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
(3) preparation of microbial activity filler:
(a) it is 90kg/m that the microbial bacteria suspension of being prepared by step (2) sprays density
3, porosity is 96%, and particle diameter is 10mm polyurethane particles surface, and the ratio of bacteria suspension and polyurethane particles is 100:10; (b) polyurethane particles of the hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening being put into impeller rotation mixes; (c) again to polyurethane particles surface spraying microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
Preferred repeating step (a)-(c) 3 time, makes equal appendix in polyurethane particles surface and micropore have bacterial cell, obtains microbial activity filler.
The water pipe blackening cell concentration 5.0 * 10 of hot bacterium ZDL1 of dwelling in described microbial activity filler
10cFU/g
(polyurethane particles).
Embodiment 4: a kind of dwell preparation method of hot bacterium active filler of water pipe blackening that sulphur-containing substance purifies medium/high sulphur-containing exhaust gas that removes
(1) inclined-plane is cultivated: cultivate and prepare the water pipe blackening hot bacterium ZDL1 slant strains of dwelling;
Slant medium consists of: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 ℃ of sterilizing 30min.
(2) preparation of bacteria suspension: the water pipe blackening of respectively being prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, the separated cell concentration that obtains is the 0.8g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
The water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, 60 ℃ of incubation time 48h of cultivation temperature, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.8g/L water pipe blackening hot bacterium ZDL1 bacteria suspension of dwelling;
Liquid fermentation medium consist of powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
Without consisting of of bacterial nutrient solution: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
(3) preparation of microbial activity filler:
(a) it is 100kg/m that the microbial bacteria suspension of being prepared by step (2) sprays density
3, porosity is 78%, and particle diameter is 10mm haydite surface, and the ratio of bacteria suspension and haydite is 100:35; (b) haydite of the hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening being put into impeller rotation mixes; (c) again to haydite surface spraying microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
Preferred repeating step (a)-(c) 3 time, makes equal appendix in haydite surface and micropore have bacterial cell, obtains microbial activity filler.
The water pipe blackening cell concentration 5.0 * 10 of hot bacterium ZDL1 of dwelling in described microbial activity filler
8cFU/g
(haydite).
Embodiment 5: the method for gas is processed in the hot bacterium active filler of dwelling
(1) appendix there is is the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 7 days;
Described SO
2concentration be 100ppm;
(2) the microbial activity filler after activation is placed in to bioreactor, pass into the gas of sulfur-containing foul material, the water pipe blackening sulfur-containing foul material in hot bacterium (Thermus Scotoductus) ZDL1 purifying exhaust air of dwelling, microbial activity packing volume is 5m
3, the total concentration of sulfide is 200ppm, gas flow rate is 20m
3/ h, purification temperature is 60 ℃; Regularly to microbial activity filling surface spraying nutrient solution, maintain the activity of microbial activity filler, spray frequency is to spray 1 time for 3 days, and each spray rate is 50L/min, and each spray time is 20min;
Consisting of of described nutrient solution: powdered beef 5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5; Follow the tracks of the degradation capability that detects sulfur-containing foul material.
The present invention adopts gas chromatography to follow the tracks of and detects sulfur-containing compound; Described gas chromatography is: adopt the analysis of Agilent GC-6890N gas chromatograph to measure sulfide concentration, chromatographic column is DB-1701 capillary column (30m * 0.32mm * 0.25 μ m), vaporizer, FPD detector, column temperature are set to respectively 100 ℃, 200 ℃, 50 ℃, sample size 100 μ L, N
2flow velocity is 1.7mL/min, and shunt volume is 34.6mL/min.
Embodiment 6: result of use
(1) appendix of embodiment 3 preparation there is is the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 7 days;
Described SO
2concentration be 100ppm;
(2) the microbial activity filler after activation is placed in to bioreactor, the sulfur dioxide gas that lignin-sludge factory sludge drying produces, microbial activity packing volume is 5m
3, the total concentration of sulfide is 248.56ppm, gas flow rate is 20m
3/ h, the time of staying is 1.0mins, purification temperature is 60 ℃; Regularly to microbial activity filling surface spraying nutrient solution, maintain the activity of microbial activity filler, spray frequency is to spray 1 time for 5 days, and each spray rate is 50L/min, and each spray time is 20min;
Consisting of of described nutrient solution: powdered beef 5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5.
After testing, after processing, the sulfur dioxide concentration of giving vent to anger is 7.3ppm, the discharge standard of air pollutants of formulating lower than country, and clearance reaches 97%.
Embodiment 7: result of use
(1) appendix of embodiment 4 preparation there is is the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 7 days;
Described SO
2concentration be 100ppm;
(2) the microbial activity filler after activation is placed in to bioreactor, the sulphur-containing exhaust gas that lignin-sludge mummification produces, the concentration of sulfur dioxide and carbon disulfide is respectively 27.08ppm and 46ppm, and gas flow is 70m
3/ h, the time of staying is 1.0min, under room temperature condition, operates, and regularly to microbial activity filling surface spraying nutrient solution, maintains the activity of microbial activity filler, and spray frequency is to spray 1 time for 5 days, and each spray rate is 50L/min, and each spray time is 20min;
Consisting of of described nutrient solution: powdered beef 5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5.
After testing, the clearance of sulfur dioxide is 95%, and the clearance of carbon disulfide is 80%.
Claims (9)
1. a preparation method who contains the active filler of the hot bacterium that dwells, is characterized in that, comprises the steps:
(1) inclined-plane is cultivated: cultivate and prepare the hot bacterium slant strains of dwelling
(2) preparation of bacteria suspension: the hot bacterium inclined-plane of dwelling of preparation is expanded to fermented and cultured to fermentation tank step by step by 5-20% inoculum concentration, 60 ℃ of cultivation temperature, incubation time 48h, ventilation 5-200L/min, dissolved oxygen 30%, tank pressure 0.03-0.10Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 2000-8000rpm, centrifugal concentrating 5-20min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.4-0.8g/L hot bacterium bacteria suspension of dwelling;
(3) preparation of microbial activity filler:
(a) the microbial bacteria suspension of being prepared by step (2) sprays the surface of porous material;
(b) the corrosion-resistant and high-temperature resistant porous material piece with the hot bacterium bacterial cell of dwelling being put into impeller rotation mixes;
(c) again to corrosion-resistant and high-temperature resistant porous material surface spray microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
The described hot bacterium that dwells is water pipe blackening hot bacterium (Thermus Scotoductus) ZDL1 of dwelling, deposit number CGMCC No.7430.
2. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: the described hot bacterium slant medium of dwelling consists of: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, agar 15g, pH value 6.0-7.5,121 ℃ of sterilizing 30min.
3. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: the consisting of of the described hot bacteria liquid fermentation medium of dwelling: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5,121 ℃ of sterilizing 30min.
4. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: described consisting of without bacterial nutrient solution: powdered beef 3.0-5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5,121 ℃ of sterilizing 30min.
5. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: described porous material is at least one in haydite, granular activated carbon, volcanic rock, sponge.
6. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: described porous material density is 0.7-1.0kg/m
3, porosity is 50-96%, particle diameter is 5-50mm.
7. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: the dwell mass ratio of hot bacterium ZDL1 bacteria suspension and porous material of described water pipe blackening is 50-300: 10.
8. the preparation method of a kind of active filler that contains the hot bacterium that dwells as claimed in claim 1, is characterized in that: concrete steps are:
(1) inclined-plane is cultivated: cultivate and prepare the hot bacterium ZDL1 slant strains of dwelling;
Slant medium consists of: powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 ℃ of sterilizing 30min.
(2) preparation of bacteria suspension: the hot bacterium ZDL1 inclined-plane thalline of dwelling prepared by step (1) is by 10% inoculum concentration fermented and cultured step by step, the separated cell concentration that obtains is the 0.6g/L hot bacterium ZDL1 bacteria suspension of dwelling;
The hot bacterium ZDL1 collecting cells method of dwelling is as follows;
The hot bacterium ZDL1 inclined-plane thalline of dwelling prepared by step (1) is by 10% inoculum concentration fermented and cultured step by step, 60 ℃ of incubation time 48h of cultivation temperature, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 ℃, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is used respectively without bacterial nutrient solution dilution, obtains cell concentration and be the 0.6g/L hot bacterium ZDL1 bacteria suspension of dwelling;
Liquid fermentation medium consist of powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
Without consisting of of bacterial nutrient solution: powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, 6.5,121 ℃ of sterilizing 30min of pH value;
(3) preparation of microbial activity filler:
(a) it is 200kg/m that the microbial bacteria suspension of being prepared by step (2) sprays density
3, porosity is 52%, and particle diameter is 200mm volcanic rock particle surface, and the ratio of bacteria suspension and volcanic rock particle is 100:80; (b) by put into impeller rotation with the volcanic rock grain particle of the hot bacterium ZDL1 bacterial cell of dwelling, mix; (c) again to volcanic rock grain particle surface spray microbial bacteria suspension, obtain microbial activity filler, 4 ℃ save backup;
Repeating step (a)-(c) 3 time, makes equal appendix in volcanic rock grain particle surface and micropore have bacterial cell, obtains microbial activity filler.
Dwell in the described microbial activity filler cell concentration 5.0 * 10 of hot bacterium ZDL1
7cFU/g
(volcanic rock particle).
9. the application of the active filler that prepared by the preparation method of the active filler that contains the hot bacterium that dwells as claimed in claim 1 in purifying medium/high sulphur-containing exhaust gas.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3860911B2 (en) * | 1998-06-05 | 2006-12-20 | 三菱重工業株式会社 | Flue gas desulfurization method and apparatus |
| CN103084059A (en) * | 2011-11-08 | 2013-05-08 | 财团法人交大思源基金会 | Biological compounds and methods for reducing hydrogen sulfide |
| CN103266099A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 active filler and preparation method thereof |
| CN103272477A (en) * | 2013-05-29 | 2013-09-04 | 中国科学院生态环境研究中心 | Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof |
-
2013
- 2013-12-18 CN CN201310697441.7A patent/CN103691305B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3860911B2 (en) * | 1998-06-05 | 2006-12-20 | 三菱重工業株式会社 | Flue gas desulfurization method and apparatus |
| CN103084059A (en) * | 2011-11-08 | 2013-05-08 | 财团法人交大思源基金会 | Biological compounds and methods for reducing hydrogen sulfide |
| CN103266099A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 active filler and preparation method thereof |
| CN103272477A (en) * | 2013-05-29 | 2013-09-04 | 中国科学院生态环境研究中心 | Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof |
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| CN103691305B (en) | 2016-03-30 |
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