CN103691305B - A kind of preparation method of the active filler containing the hot bacterium that dwells - Google Patents
A kind of preparation method of the active filler containing the hot bacterium that dwells Download PDFInfo
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- CN103691305B CN103691305B CN201310697441.7A CN201310697441A CN103691305B CN 103691305 B CN103691305 B CN 103691305B CN 201310697441 A CN201310697441 A CN 201310697441A CN 103691305 B CN103691305 B CN 103691305B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention discloses a kind of preparation method of the active filler containing the hot bacterium that dwells.Is water pipe blackening involved in the present invention dwelt hot bacterium (Thermus? Scotoductus) ZDL1, for obtaining isolate from the medium/high bioreactor of Ecological Environment Research Center, Chinese Academy of Sciences's water pollution control laboratory treatment containing the sulphur-containing exhaust gas such as sulfur dioxide, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, does is deposit number CGMCC? No.7430.The active filler specific area containing the hot bacterium that dwells having the present invention to obtain is large, and porosity is high, good permeability, and resistance is little; Bacterial cell appendix amount is high, not easily runs off; The appendix bacterium that has superiority dwells hot bacterium ZDL1, has the efficiency of the higher sulfur-containing foul material such as degraded sulfur dioxide, carbon disulfide; And the laundering period of dominant bacteria is short, degradation reaction can be started fast, be applied in the waste gas bioreactor of purification medium/high sulfur-containing foul material, can the starting period be shortened.
Description
Technical field:
The invention belongs to field of environmental improvement, be specifically related to a kind of preparation method of the active filler containing the hot bacterium that dwells.
Background technology:
In the processes such as sewage disposal, sludge anhydration burning, compost, can produce the waste gas of temperature higher (35 ~ 100 DEG C), this medium/high exhaust gas constituents is complicated, and wherein sulfur dioxide is one of main material.Waste gas containing sulfur dioxide spills in air, along with the diffuses flow of air is to everywhere, pollutes surrounding environment, produces harm to health.At present, the improvement of waste gas causes people more and more to pay close attention to, and many countries have all formulated relevant discharge standard, the strict discharge controlling pollutant in waste gas, and require that enterprise before discharging, take corresponding control measures.To 33 kinds of atmosphere pollutions in country " discharge standard of air pollutants " (GB16297-1996) that China came into effect in calendar year 2001, the emission limit of the pollutants such as such as sulfur dioxide has made strict regulation.
Compared with other physical chemistry waste gas processing method, bioremediation has the advantages such as cost is low, easy and simple to handle, technology clean, non-secondary pollution because of it, becomes the focus of current research after the nineties.Biological ratio juris utilizes the biological oxidation of microorganism that sulphur-containing substance in waste gas is converted into the process of sulfate or sulphur simple substance.Biological method is suitable for the waste gas processing low concentration, atm number.
But biological treatment technology for waste gas conventional is at present all the material under process normal temperature, usually adopt spraying cooling water to the method for reprocessing after waste gas cooling to the process of high-temp waste gas.Like this, not only increase energy consumption, and need the new a large amount of waste water produced of process, increase processing cost.
Therefore, find the hot bacterium that dwells that can adapt to the existence of medium/high environment, utilize hot bacterium centering/high-temp waste gas of dwelling directly to process, without the need to cooling system, thus while reaching exhaust-gas treatment requirement, simplify processes technique, reduces costs.
In addition, all fill a certain amount of carrier in prior art for the treatment of in the bioreactor of waste gas, attached growth of microorganisms is at carrier surface process waste gas.So suitable carrier should have certain surface nature, be applicable to attachment and the growth of microorganism.When the waste gas processed is the sulfurous gas of medium/high, used carrier also needs to have high temperature resistant and corrosion resistant character.Conventional exhaust-gas treatment bioreactor adopts the organic matters such as composting material, wood chip, bark as carrier, and this kind of organic carrier specific area is little, not heatproof, perishable, and the pressure loss is large, and service life is short; And the inert carrier surfaces such as volcanic rock, haydite, active carbon have loose structure, specific area is large, and can tolerate higher temperature.
Summary of the invention:
The problem to be solved in the present invention is to provide a kind of preparation method that can process the active filler containing the hot bacterium that dwells of medium/high waste gas.
Concrete preparation process is as follows:
(1) inclined-plane is cultivated: cultivate preparation and to dwell hot bacterium slant strains;
Described hot bacterium slant medium of dwelling consists of: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, agar 15g, pH value 6.0-7.5,121 DEG C of sterilizing 30min.
(2) preparation of bacteria suspension: the hot bacterium inclined-plane thalline of dwelling step (1) prepared respectively is by 5-20% inoculum concentration fermented and cultured step by step, and being separated and obtaining cell concentration is that 0.4-0.8g/L is dwelt hot bacterium bacteria suspension;
Hot bacterium collecting cells method of dwelling is as follows;
The hot bacterium inclined-plane thalline of dwelling step (1) prepared is by 5-20% inoculum concentration fermented and cultured step by step, cultivation temperature 55-60 DEG C of incubation time 36-48h, ventilation 5-200L/min, dissolved oxygen 30%, tank pressure 0.03-0.10Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and obtaining cell concentration is that 0.4-0.8g/L is dwelt hot bacterium bacteria suspension;
Consisting of of described hot bacteria liquid fermentation medium of dwelling: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5,121 DEG C of sterilizing 30min;
Consisting of of described hot bacterium sterile nutrient solution of dwelling: powdered beef 3.0-5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5,121 DEG C of sterilizing 30min;
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (2) is sprayed the surface of porous material by (); B corrosion-resistant and high-temperature resistant porous material block (or particle) with hot bacterium bacterial cell of dwelling is put into impeller and is rotated mixing by (); C (), again to corrosion-resistant and high-temperature resistant porous material surface spray microbial bacteria suspension, obtain microbial activity filler, 4 DEG C save backup;
Preferred repetition step (a)-(c) 2-3 time, makes equal appendix in material surface and micropore have bacterial cell, obtains microbial activity filler.
Described porous material is one or more in haydite, granular activated carbon, volcanic rock, sponge;
Described porous material density is 0.7-1.0kg/m
3, porosity is 50-96%, and particle diameter is 5-50mm;
The described hot bacterium that dwells is specially water pipe blackening and dwells hot bacterium (ThermusScotoductus) ZDL1, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 8th, 2013, deposit number is CGMCCNo.7430, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101;
This bacterial strain feature is as follows:
This bacterial strain colony colour on LB solid plate is white, opaque, out-of-shape, and the smooth of the edge is moistening.Thalline is elongated rod shape, size is 0.5 – 1.5 × 2.5 – 15.0 μm, atrichia and spore, Gram-negative, catalase and oxidase positive, can nitrate be utilized, responsive to penicillin, erythromycin, tetracycline, chloramphenicol and streptomysin, but there is anti-PB and rifampin characteristic.Optimum growth temperature is 30-100 DEG C;
The filler prepared by this bacterium is 0.001-200m at volume
3, the total concentration of sulfide is 30-250ppm, and gas flow rate is 0.05-10000m
3/ h, purification temperature is 50-65 DEG C, reaches more than 85% under time of staying 15s-3min condition to the degradation efficiency of sulfide.
Described water pipe blackening dwells hot bacterium ZDL1 with the form appendix of bacteria suspension on filler;
The dwell mass ratio of hot bacterium ZDL1 bacteria suspension and corrosion-resistant, high temperature resistant porous material of described water pipe blackening is 50-300: 10;
In described hot bacterium active filler of dwelling, the dwell cell concentration of hot bacterium ZDL1 of water pipe blackening is greater than 10
6cFU/g
(active filler).
Odorous gas process is carried out in described hot bacterium active filler of dwelling, particularly at degraded SO
2application in gas, concrete steps are as follows:
(1) there is by appendix the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening to be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 1-30 days; Activation temperature 45-55 DEG C.
Described SO
2concentration be 50-200ppm;
(2) the microbial activity filler after activation is placed in bioreactor, passes into the gas of sulphur-containing substance, to dwell the sulphur-containing substance in hot bacterium ZDL1 purifying exhaust air with water pipe blackening, microbial activity packing volume is 0.001-200m
3, the total concentration of sulfide is 30-250ppm, and gas flow rate is 0.05-10000m
3/ h, purification temperature is 50-65 DEG C; Regularly maintain the activity of microbial activity filler to microbial activity filling surface spraying nutrient solution, spray frequency is spray 1 time in 1-5 days, and each spray rate is 1.0-200L/min, and each spray time is 20-120min;
Consisting of of described nutrient solution: powdered beef 3.0-5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5; The degradation capability of tracing detection sulfur-containing foul material.
The present invention adopts gas chromatography tracing detection sulfur-containing compound; Described gas chromatography is: adopt Agilent GC-6890N chromatographic to measure sulfide concentration, chromatographic column is DB-1701 capillary column (30m × 0.32mm × 0.25 μm), vaporizer, FPD detector, column temperature are set to 100 DEG C, 200 DEG C, 50 DEG C respectively, sample size 100 μ L, N
2flow velocity is 1.7mL/min, and shunt volume is 34.6mL/min.
Beneficial effect:
1. hot bacterium active filler appendix of the dwelling of the present invention bacterium water pipe blackening that has superiority is dwelt hot bacterium ZDL1, has the efficiency of the higher sulfur-containing foul material such as degraded sulfur dioxide, carbon disulfide; And the laundering period of dominant bacteria is short, degradation reaction can be started fast, be applied in the waste gas bioreactor of purification medium/high sulfur-containing foul material, can the starting period be shortened.
2. utilize the effect of sedimentation, attachment and the multiple mechanics such as centrifugal, the blackening of superior strain water pipe is dwelt hot bacterium ZDL1 appendix on corrosion-resistant and high-temperature resistant porous material, form microbial activity filler, bacterial cell can not only be attached to filling surface equably, and can enter in the hole of filler inside, make the bacterial cell appendix amount of microbial activity filler high, not easily run off from bioreactor.
3., through overactivation before hot bacterium active filler of dwelling uses, bioreactor can be made to start fast.The bacterial cell enzyme of appendix is lived stable, can maintain the clean-up effect of sulphur-containing exhaust gas steady in a long-term.
4., for rough surface, the porous of the carrier material of microbial activity filler, be suitable for bacterial cell attachment; Specific area is large, good permeability, and resistance is little, is conducive to bacterial cell and contacts with the sulphur-containing substance in waste gas, improves mass-transfer efficiency.
5. filler used in the present invention is high temperature resistant, corrosion-resistant, and be applied in the bioreactor of purification medium/high sulphur-containing exhaust gas, the pressure loss is little, not perishable distortion, can Long-Time Service and the physical condition adapting to Various Complex.
6. the active filler prepared by the present invention is 30-250ppm in the total concentration of sulfide, and gas flow rate is 0.05-10000m
3/ h, purification temperature is 50-65 DEG C, reaches more than 85% under time of staying 15s-3min condition to the degradation efficiency of sulfide.
Detailed description of the invention:
Embodiment 1: a kind of preparation method removing the hot bacterium active filler of dwelling of sulphur-containing substance purification medium/high sulphur-containing exhaust gas
(1) inclined-plane is cultivated: cultivate and prepare water pipe blackening and to dwell hot bacterium ZDL1 slant strains;
Slant medium consists of: powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 DEG C of sterilizing 30min.
(2) preparation of bacteria suspension: hot bacterium ZDL1 inclined-plane thalline of water pipe blackening prepared by step (1) being dwelt respectively is by 10% inoculum concentration fermented and cultured step by step, and being separated and obtaining cell concentration is that the blackening of 0.6g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, cultivation temperature 60 DEG C of incubation time 48h, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and obtaining cell concentration is that the blackening of 0.6g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Liquid fermentation medium consist of powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
Consisting of of sterile nutrient solution: powdered beef 3.5g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (2) is sprayed density by () is 200kg/m
3, porosity is 52%, and particle diameter is 200mm volcanic rock particle surface, and the ratio of bacteria suspension and volcanic rock particle is 100:80; B the volcanic rock grain particle of hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening is put into impeller and is rotated mixing by (); C (), again to volcanic rock grain particle surface spray microbial bacteria suspension, obtain microbial activity filler, 4 DEG C save backup;
Repeat step (a)-(c) 3 times, make equal appendix in volcanic rock grain particle surface and micropore have bacterial cell, obtain microbial activity filler.
In described microbial activity filler, water pipe blackening is dwelt the cell concentration 5.0 × 10 of hot bacterium ZDL1
7cFU/g
(volcanic rock particle).
Embodiment 2: a kind of water pipe blackening removing sulphur-containing substance purification medium/high sulphur-containing exhaust gas is dwelt the preparation method of hot bacterium active filler
(1) inclined-plane is cultivated: cultivate and prepare water pipe blackening and to dwell hot bacterium ZDL1 slant strains;
Slant medium consists of: powdered beef 4.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 DEG C of sterilizing 30min.
(2) preparation of bacteria suspension: hot bacterium ZDL1 inclined-plane thalline of water pipe blackening prepared by step (1) being dwelt respectively is by 10% inoculum concentration fermented and cultured step by step, and being separated and obtaining cell concentration is that the blackening of 0.6g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, cultivation temperature 60 DEG C of incubation time 48h, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and obtaining cell concentration is that the blackening of 0.6g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Liquid fermentation medium consist of powdered beef 4.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
Consisting of of sterile nutrient solution: powdered beef 4.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (2) is sprayed density by () is 1.0kg/m
3, porosity is 85%, and particle diameter is 20mm seed activity carbon surface, and the ratio of bacteria suspension and granular activated carbon is 100:15; B the granular activated carbon of hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening is put into impeller and is rotated mixing by (); C (), again to granular activated carbon surface spraying microbial bacteria suspension, obtain microbial activity filler, 4 DEG C save backup;
Preferred repetition step (a)-(c) 3 times, makes equal appendix in seed activity carbon surface and micropore have bacterial cell, obtains microbial activity filler.
In described microbial activity filler, water pipe blackening is dwelt the cell concentration 5.0 × 10 of hot bacterium ZDL1
8cFU/g
(granular activated carbon).
Embodiment 3: a kind of preparation method removing the hot bacterium active filler of dwelling of sulphur-containing substance purification medium/high sulphur-containing exhaust gas
(1) inclined-plane is cultivated: cultivate and prepare water pipe blackening and to dwell hot bacterium ZDL1 slant strains;
Slant medium consists of: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 DEG C of sterilizing 30min.
(2) preparation of bacteria suspension: hot bacterium ZDL1 inclined-plane thalline of water pipe blackening prepared by step (1) being dwelt respectively is by 10% inoculum concentration fermented and cultured step by step, and being separated and obtaining cell concentration is that the blackening of 0.6g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, cultivation temperature 60 DEG C of incubation time 48h, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and obtaining cell concentration is that the blackening of 0.6g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Liquid fermentation medium consist of powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
Consisting of of sterile nutrient solution: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (2) is sprayed density by () is 90kg/m
3, porosity is 96%, and particle diameter is 10mm polyurethane particles surface, and the ratio of bacteria suspension and polyurethane particles is 100:10; B the polyurethane particles of hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening is put into impeller and is rotated mixing by (); C (), again to polyurethane particles surface spraying microbial bacteria suspension, obtain microbial activity filler, 4 DEG C save backup;
Preferred repetition step (a)-(c) 3 times, makes equal appendix in polyurethane particles surface and micropore have bacterial cell, obtains microbial activity filler.
In described microbial activity filler, water pipe blackening is dwelt the cell concentration 5.0 × 10 of hot bacterium ZDL1
10cFU/g
(polyurethane particles).
Embodiment 4: a kind of water pipe blackening removing sulphur-containing substance purification medium/high sulphur-containing exhaust gas is dwelt the preparation method of hot bacterium active filler
(1) inclined-plane is cultivated: cultivate and prepare water pipe blackening and to dwell hot bacterium ZDL1 slant strains;
Slant medium consists of: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.0-7.5, agar 15g, 121 DEG C of sterilizing 30min.
(2) preparation of bacteria suspension: hot bacterium ZDL1 inclined-plane thalline of water pipe blackening prepared by step (1) being dwelt respectively is by 10% inoculum concentration fermented and cultured step by step, and being separated and obtaining cell concentration is that the blackening of 0.8g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Water pipe blackening hot bacterium ZDL1 collecting cells method of dwelling is as follows;
Water pipe blackening prepared by step (1) is dwelt hot bacterium ZDL1 inclined-plane thalline by 10% inoculum concentration fermented and cultured step by step, cultivation temperature 60 DEG C of incubation time 48h, ventilation 50L/min, dissolved oxygen 30%, tank pressure 0.05Mpa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 6000rpm, centrifugal concentrating 10min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and obtaining cell concentration is that the blackening of 0.8g/L water pipe is dwelt hot bacterium ZDL1 bacteria suspension;
Liquid fermentation medium consist of powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
Consisting of of sterile nutrient solution: powdered beef 5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5,121 DEG C of sterilizing 30min;
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (2) is sprayed density by () is 100kg/m
3, porosity is 78%, and particle diameter is 10mm haydite surface, and the ratio of bacteria suspension and haydite is 100:35; B the haydite of hot bacterium ZDL1 bacterial cell of dwelling with water pipe blackening is put into impeller and is rotated mixing by (); C (), again to haydite surface spraying microbial bacteria suspension, obtain microbial activity filler, 4 DEG C save backup;
Preferred repetition step (a)-(c) 3 times, makes equal appendix in haydite surface and micropore have bacterial cell, obtains microbial activity filler.
In described microbial activity filler, water pipe blackening is dwelt the cell concentration 5.0 × 10 of hot bacterium ZDL1
8cFU/g
(haydite).
Embodiment 5: the method for hot bacterium active filler process gas of dwelling
(1) there is by appendix the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening to be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 7 days;
Described SO
2concentration be 100ppm;
(2) the microbial activity filler after activation is placed in bioreactor, pass into the gas of sulfur-containing foul material, to dwell the sulfur-containing foul material in hot bacterium (ThermusScotoductus) ZDL1 purifying exhaust air with water pipe blackening, microbial activity packing volume is 5m
3, the total concentration of sulfide is 200ppm, and gas flow rate is 20m
3/ h, purification temperature is 60 DEG C; Regularly maintain the activity of microbial activity filler to microbial activity filling surface spraying nutrient solution, spray frequency is spray 1 time for 3 days, and each spray rate is 50L/min, and each spray time is 20min;
Consisting of of described nutrient solution: powdered beef 5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5; The degradation capability of tracing detection sulfur-containing foul material.
The present invention adopts gas chromatography tracing detection sulfur-containing compound; Described gas chromatography is: adopt Agilent GC-6890N chromatographic to measure sulfide concentration, chromatographic column is DB-1701 capillary column (30m × 0.32mm × 0.25 μm), vaporizer, FPD detector, column temperature are set to 100 DEG C, 200 DEG C, 50 DEG C respectively, sample size 100 μ L, N
2flow velocity is 1.7mL/min, and shunt volume is 34.6mL/min.
Embodiment 6: result of use
(1) there is by appendix prepared by embodiment 3 the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening to be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 7 days;
Described SO
2concentration be 100ppm;
(2) the microbial activity filler after activation is placed in bioreactor, the sulfur dioxide gas that lignin-sludge factory sludge drying produces, microbial activity packing volume is 5m
3, the total concentration of sulfide is 248.56ppm, and gas flow rate is 20m
3/ h, the time of staying is 1.0mins, and purification temperature is 60 DEG C; Regularly maintain the activity of microbial activity filler to microbial activity filling surface spraying nutrient solution, spray frequency is spray 1 time for 5 days, and each spray rate is 50L/min, and each spray time is 20min;
Consisting of of described nutrient solution: powdered beef 5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5.
After testing, after process, sulfur dioxide concentration of giving vent to anger is 7.3ppm, and lower than the discharge standard of air pollutants that country formulates, clearance reaches 97%.
Embodiment 7: result of use
(1) there is by appendix prepared by embodiment 4 the dwell microbial activity filler of hot bacterium ZDL1 of water pipe blackening to be placed in a container, pass into SO
2gas, with SO
2gas is sulphur source, activation microbial activity filler; Soak time is 7 days;
Described SO
2concentration be 100ppm;
(2) the microbial activity filler after activation is placed in bioreactor, the sulphur-containing exhaust gas that lignin-sludge mummification produces, the concentration of sulfur dioxide and carbon disulfide is respectively 27.08ppm and 46ppm, and gas flow is 70m
3/ h, the time of staying is 1.0min, operates under room temperature condition, regularly maintains the activity of microbial activity filler to microbial activity filling surface spraying nutrient solution, and spray frequency is spray 1 time for 5 days, and each spray rate is 50L/min, and each spray time is 20min;
Consisting of of described nutrient solution: powdered beef 5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000ml, pH value 6.5.
After testing, the clearance of sulfur dioxide is 95%, and the clearance of carbon disulfide is 80%.
Claims (8)
1. a preparation method for the active filler containing the hot bacterium that dwells, is characterized in that, comprise the steps:
(1) inclined-plane is cultivated: cultivate preparation and to dwell hot bacterium slant strains
(2) preparation of bacteria suspension: the hot bacterium inclined-plane of dwelling of preparation is expanded fermented and cultured to fermentation tank step by step by 5-20% inoculum concentration, cultivation temperature 60 DEG C, incubation time 48h, ventilation 5-200L/min, dissolved oxygen 30%, tank pressure 0.03-0.10MPa, zymotic fluid is placed in refrigerated centrifuge in 4 DEG C, 2000-8000rpm, centrifugal concentrating 5-20min, abandoning supernatant, precipitation is respectively with sterile nutrient solution dilution, and obtaining cell concentration is that 0.4-0.8g/L is dwelt hot bacterium bacteria suspension;
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (2) is sprayed the surface of porous material by ();
B corrosion-resistant and high-temperature resistant porous material block with hot bacterium bacterial cell of dwelling is put into impeller and is rotated mixing by ();
C (), again to corrosion-resistant and high-temperature resistant porous material surface spray microbial bacteria suspension, obtain microbial activity filler, 4 DEG C save backup;
The described hot bacterium that dwells is that water pipe blackening is dwelt hot bacterium (ThermusScotoductus) ZDL1, deposit number CGMCCNo.7430.
2. the preparation method of a kind of active filler containing the hot bacterium that dwells as claimed in claim 1, is characterized in that: described inclined-plane is cultivated the slant medium used and consisted of: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000mL, agar 15g, pH value 6.0-7.5,121 DEG C of sterilizing 30min.
3. the preparation method of a kind of active filler containing the hot bacterium that dwells as claimed in claim 1, is characterized in that: consisting of of the liquid fermentation medium that described fermented and cultured uses: powdered beef 3.0-5.0g, Na
2s
2o
35H
2o5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeSO
44H
2o0.01g, NaHCO
31.0g, water 1000mL, pH value 6.0-7.5,121 DEG C of sterilizing 30min.
4. the preparation method of a kind of active filler containing the hot bacterium that dwells as claimed in claim 1, is characterized in that: consisting of of described sterile nutrient solution: powdered beef 3.0-5.0g, KH
2pO
42.0g, KNO
32.0g, NH
4cl0.5g, MgCl
26H
2o0.5g, FeCl
27H
2o0.01g, NaHCO
31.0g, water 1000mL, pH value 6.0-7.5,121 DEG C of sterilizing 30min.
5. the preparation method of a kind of active filler containing the hot bacterium that dwells as claimed in claim 1, is characterized in that: described porous material is at least one in haydite, granular activated carbon, volcanic rock, sponge.
6. the preparation method of a kind of active filler containing the hot bacterium that dwells as claimed in claim 1, is characterized in that: described porous material density is 0.7-1.0kg/m
3, porosity is 50-96%, and particle diameter is 5-50mm.
7. the preparation method of a kind of active filler containing the hot bacterium that dwells as claimed in claim 1, is characterized in that: the dwell mass ratio of hot bacterium ZDL1 bacteria suspension and porous material of described water pipe blackening is 50-300: 10.
8. the active filler that prepared by the preparation method of the active filler containing the hot bacterium that dwells as claimed in claim 1 is purifying the application in medium/high sulphur-containing exhaust gas.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3860911B2 (en) * | 1998-06-05 | 2006-12-20 | 三菱重工業株式会社 | Flue gas desulfurization method and apparatus |
| CN103084059A (en) * | 2011-11-08 | 2013-05-08 | 财团法人交大思源基金会 | Biological compounds and methods for reducing hydrogen sulfide |
| CN103266099A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 active filler and preparation method thereof |
| CN103272477A (en) * | 2013-05-29 | 2013-09-04 | 中国科学院生态环境研究中心 | Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof |
-
2013
- 2013-12-18 CN CN201310697441.7A patent/CN103691305B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3860911B2 (en) * | 1998-06-05 | 2006-12-20 | 三菱重工業株式会社 | Flue gas desulfurization method and apparatus |
| CN103084059A (en) * | 2011-11-08 | 2013-05-08 | 财团法人交大思源基金会 | Biological compounds and methods for reducing hydrogen sulfide |
| CN103266099A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 active filler and preparation method thereof |
| CN103272477A (en) * | 2013-05-29 | 2013-09-04 | 中国科学院生态环境研究中心 | Compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application thereof |
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| CN103691305A (en) | 2014-04-02 |
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