CN103266075B - Human pallid bacillus and the application in removing sulfur-containing foul material thereof - Google Patents
Human pallid bacillus and the application in removing sulfur-containing foul material thereof Download PDFInfo
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- CN103266075B CN103266075B CN201310206408.XA CN201310206408A CN103266075B CN 103266075 B CN103266075 B CN 103266075B CN 201310206408 A CN201310206408 A CN 201310206408A CN 103266075 B CN103266075 B CN 103266075B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention discloses a strain human pallid bacillus and the application in raw removing sulfur-containing foul material thereof.Human pallid bacillus (Ochrobactrum anthropi) SL1 involved in the present invention, for from Ecological Environment Research Center, Chinese Academy of Sciences's water pollution control laboratory treatment containing the reactor of the odorant such as H2S obtaining separate bacterium, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC No.7400.This bacterial strain can be prepared as microbial activity filler, gas for purifying sulfur-containing foul material, solve the odor pollution problem that the processes such as sewage, Treatment of Sludge, garbage disposal and compost produce, the sulfur-containing foul mass degradation efficiency such as hydrogen sulfide, methanthiol, methyl sulfide can be reached more than 90%.
Description
Technical field:
The invention belongs to field of environmental improvement, be specifically related to a kind of microorganism removing sulfur-containing foul material and answer
With.
Background technology:
Substantial amounts of odorous gas can be produced, sulfuration during sewage, Treatment of Sludge, garbage disposal and compost etc.
The sulphur-containing substances such as hydrogen, mercaptan, thioether, Carbon bisulfide are malodoring substance common in odorous gas.Odorant
Spill in air, along with the flowing of air is diffused into everywhere, surrounding is polluted, to health
Produce harm.At present, the improvement of air pollution and foul smell causes people more and more to pay close attention to, and many countries are all
Formulate strict discharge standard.The discharge of pollutant in strict control waste gas, and require that enterprise is at exhaust emission
Before thing, take corresponding control measures." odorant pollutant discharges in the country that China came into effect in 1993
Standard " in (GB14554-93) discharge to volatile organic contaminant and odorant be made that strict rule
Fixed.
Compared with other physical chemistry waste gas processing method, bioremediation has low cost, operation letter because of it
Just, the advantage such as technology cleaning, non-secondary pollution, become the focus of research after the nineties.Bioanalysis former
Reason is that the odorant of sulfur-bearing is converted into sulfate or the mistake of sulfur simple substance by the biological oxidation utilizing microorganism
Journey.
But, the usual apposition growth of microorganism, at carrier surface, needs periodically to load when running bioreactor
Body spraying nutrient solution with maintain microorganism growth needed for nutrient and moisture.During spray, microorganism
Easily come off from carrier surface, flow out from the bioreactor purifying waste gas with unnecessary nutritional solution, cause stream
Lose, cause the problem that the treatment effect of sulfur-containing foul material reduces.Further, carrier generally use composting material,
Wood chip, bark, Raschig ring, haydite etc..The specific surface area of the carriers such as composting material, wood chip, bark is little, fills out
Filling density big, the pressure loss is big, and perishable, service life is short；Raschig ring, haydite smooth surface, microorganism
It is difficult to attachment, is more easy to run off.The inoculum of the bioreactor of purifying sulfur-containing foul material mostly is sewage treatment plant
Activated sludge, the microorganism laundering period is long, and bioreactor starts slow.
Summary of the invention:
It is an object of the invention to provide a strain human pallid bacillus (Ochrobactrum anthropi) SL1 and de-
Except the application in sulfur-containing foul material.
Human pallid bacillus (Ochrobactrum anthropi) SL1 involved in the present invention, for from the Chinese Academy of Sciences
Ecological Environmental Research Center's water pollution control laboratory treatment contains H2The reactor of the odorants such as S is divided
From bacterium.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number
For CGMCC No.7400, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese science
Institute of microbiology of institute, postcode 100101.Preservation date on April 02nd, 2013.
This bacterial strain feature is as follows:
This bacterial strain colony colour on LB solid plate is colourless, circular, smooth surface, and neat in edge is wet
Profit.Thalline is shaft-like, and size is 0.5-0.8 × 3.0-3.5 μm, peritrichous, Gram-negative, contact
Enzyme and oxidase positive.Indole is negative.Glucose fermentation produces acid, it is possible to use nitrate, does not hydrolyze esculin
And gelatin.Can extensively utilize carbohydrate, aminoacid, organic acid for carbon source.Optimum growth temperature is
20-37℃。
Full-automatic magnetic beads instrument for extracting nucleic acid Smart LabAssist-16 and respective environment sample DNA is used to extract
Purpose bacterial strain DNA is extracted and purification by purification kit respectively, selects bacterial universal primers F16S-27
Carrying out PCR amplification with R16S-1492, primer sequence is respectively as follows:
F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)
R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)
PCR reaction condition is set as: 94 DEG C of denaturations 5min；94 DEG C of degeneration 30s subsequently, 56 DEG C of annealing
30s, 72 DEG C extend 90s, circulate 25 cycles；Then 72 DEG C extend 10min；Last 4 DEG C keep 10min.
Then PCR primer is connected on PMD-19T carrier, is transformed into bacillus coli DH 5 alpha competence subsequently thin
In born of the same parents.Competent cell 50 μ l after converting coats containing galactoside, isopropyl-β-d-sulfur for gala
In the LB culture medium of glucosides and ampicillin.After cultivating 12 hours, from 25 whites of each flat board picking
Single bacterial plaque carries out LB liquid medium cultivation.After cultivating 2 hours, culture fluid is carried out sequencing analysis.Record
Sequence carry out similarity merger initially with DNAMAN software, by similarity more than 97% sequence merger
Being an operating unit, the representative sequence chosen in each operating unit utilizes BLAST Yu GenBank data
16SrDNA gene order in storehouse carries out homology analysis, chooses more than 1400bp length and compares
(Clustelx1.83), use ortho position to connect (Neighbour Joining) method and carry out Phylogenetic Analysis (MEGA3.1).
Phylogenetic tree is as shown in Figure 1.
This microbial inoculum can (packaging dosage form be by test tube slant kind-shaking flask kind-seed tank-production tank-product
Liquid bacterial agent or solid absorption microbial inoculum) production technology prepare.
The invention provides the human pallid bacillus (Ochrobactrum of a strain degraded sulfur-containing foul material
Anthropi) SL1 (CGMCC No.7400), this antibacterial the microbial inoculum prepared, is 1.0-90m at gas flow3/ h,
Time of staying 1-3min, temperature 20-37 DEG C, concentration of hydrogen sulfide is 0.1-80mg/m3, methanthiol 0.1-10mg/m3,
Methyl sulfide 0.1-10mg/m3, the sulfur-containing foul mass degradation efficiency such as hydrogen sulfide, methanthiol, methyl sulfide can be reached
To more than 90%.Can be used for the gas of purifying sulfur-containing foul material, solve at sewage, Treatment of Sludge, rubbish
The odor pollution problem that the processes such as reason and compost produce.
Described human pallid bacillus (Ochrobactrum anthropi) SL1 can prepare to be adsorbed on filler becomes micro-
Biological active filling material, preferably processes sulfur-containing foul material.
Described filler is the one in light porous polyurethane foamed blocks, granular active carbon, volcanic rock, perlite
Or multiple material；
Described packing density is 0.7-1.0kg/m3, porosity is 50-96%, and particle diameter is 5-50mm；
Described microbial bacteria suspension is 50-300: 10 with the mass ratio of light porous material；
Human pallid bacillus (Ochrobactrum anthropi) SL1 cell concentration in described microbial activity filler
More than 106CFU/g(active filler)。
Described microbial activity filler preparation process is as follows:
(1) slant culture: human pallid bacillus (Ochrobactrum anthropi) SL1 slant strains is prepared in cultivation；
Described human pallid bacillus (Ochrobactrum anthropi) SL1 slant medium consists of: glucose
0.5g, KH2PO42.0g, K2HPO42.0g, KNO32.0g, NH4Cl0.5g, MgCl2·6H2O0.2g,
FeSO4·7H2O0.01g, Na2S2O3·5H2O5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,
121 DEG C of sterilizing 20min；
(2) preparation of bacteria suspension: human pallid bacillus (Ochrobactrum anthropi) prepared by step (1)
SL1 inclined-plane thalline presses 5-20% inoculum concentration fermentation culture step by step, and separating and obtaining cell concentration is 0.4-0.8g/L
Human pallid bacillus (Ochrobactrum anthropi) SL1 bacteria suspension；
Human pallid bacillus (Ochrobactrum anthropi) SL1 bacteria suspension preparation method is as follows；
Human pallid bacillus (Ochrobactrum anthropi) SL1 inclined-plane thalline ferments step by step by 5-20% inoculum concentration
Cultivate, cultivation temperature 36 DEG C, incubation time 48h, ventilation 5-200L/min, dissolved oxygen ＞ 2.0mg/L, tank
Pressure 0.03-0.10Mpa, fermentation liquid is placed in refrigerated centrifuger in 4 DEG C, 2000-8000rpm, centrifugal concentrating
5-20min, abandoning supernatant, precipitation sterile nutrient solution dilutes, it is thus achieved that cell concentration is 0.4-0.8g/L people
Anthropi (Ochrobactrum anthropi) SL1 bacteria suspension；
Consisting of of described human pallid bacillus (Ochrobactrum anthropi) SL1 liquid fermentation medium:
Glucose 0.5g, KH2PO42.0g, K2HPO42.0g, KNO32.0g, NH4Cl0.5g, MgCl2·6H2O
0.2g, FeSO4·7H2O0.01g, Na2S2O3·5H2O5.0g, water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilizing 20min；
Consisting of of described human pallid bacillus (Ochrobactrum anthropi) SL1 sterile nutrient solution: glucose
0.5g, KH2PO43.0g, K2HPO43.0g, NH4H2PO40.5g, MgCl2·6H2O0.2g, FeCl30.01g,
Water 1000ml, pH value 6.8-7.2；121 DEG C of sterilizing 20min；
(3) preparation of microbial activity filler:
A microbial bacteria suspension prepared by step (3) is sprayed the light porous material of packet or grain shape by ()
The surface of material；(b) will with human pallid bacillus (Ochrobactrum anthropi) SL1 porous material block (or
Granule) put into rotation mixing in impeller；C () is again to light porous material surface spraying microbial bacteria
Suspension, it is thus achieved that microbial activity filler, 4 DEG C save backup；
Preferably repeat step (a)-(c) 2-3 time, make equal appendix in material surface and micropore have bacterium cell, obtain
Obtain microbial activity filler；
It is another object of the present invention to utilize microbial activity filler to carry out odorous gas process, particularly in degraded
H2Application in S gas, specifically comprises the following steps that
(1) the microbial activity filler that appendix has human pallid bacillus (Ochrobactrum anthropi) SL1 is placed in
In one container, it is passed through H2S gas, with H2S gas is sulfur source, activates microbial activity filler；During activation
Between be 1-14 days；
Described H2The concentration of S is 50-200ppm；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through the gas of sulfur-containing foul material
Body, purifies the sulfur-containing foul material in waste gas with human pallid bacillus (Ochrobactrum anthropi) SL1, micro-
Biological active filling material volume is 0.001-200m3, the total concentration of sulfide is 50-200ppm, and gas flow rate is
0.05-10000m3/ h, purification temperature is 10-35 DEG C；Periodically tie up to microbial activity filling surface spraying nutrient solution
Holding the activity of microbial activity filler, spray frequency is to spray 1 time for 1-7 days, and each spray rate is
1.0-200L/min, each spray time is 20-120min；
Consisting of of described nutritional solution: glucose 0.5g, KH2PO42.0g, K2HPO42.0g, KNO3
2.0g, NH4Cl0.5g, MgCl2·6H2O0.2g, FeCl30.01g, water 1000ml, pH6.8-7.2；With
The degradation capability of track detection sulfur-containing foul material.
The present invention uses gas chromatography tracing detection sulfur-containing compound；Described gas chromatography is: use
Agilent GC-6890N chromatographic measures sulfide concentration, and chromatographic column is DB-1701 capillary
Tubing string (30m × 0.32mm × 0.25 μm), vaporizer, FPD detector, column temperature be respectively set to 100 DEG C,
250 DEG C, 220 DEG C, sample size 50 μ L, N2Flow velocity is 0.3mL/min, and total flow is 12mL/min.
Beneficial effect:
1. human pallid bacillus (Ochrobactrum anthropi) SL1 of the present invention has higher degraded hydrogen sulfide
Efficiency；And the laundering period of dominant bacteria is short, can quickly start degradation reaction, apply at purifying sulfur-containing foul
In the waste gas bioreactor of material, it is possible to shorten the starting period.
2. utilize the effect of multiple mechanics such as settling, adhere to and be centrifuged, by superior strain human pallid bacillus
(Ochrobactrum anthropi) SL1 appendix, on light porous material, forms microbial activity filler, bacterium
Cell can not only be uniformly adhered to filling surface, and can enter in the hole within filler, makes microorganism
The bacterium cell appendix amount of active filler is high, is difficult to from bioreactor run off.
3., through overactivation before microbial activity filler uses, bioreactor can be made quickly to start.The bacterium of appendix
Cellular enzymes is lived stable, it is possible to maintain the clean-up effect of sulfur-containing foul material steady in a long-term.
4., for the rough surface of carrier material of microbial activity filler, porous, be suitable to bacterium cell attachment；Ratio
Surface area is big, and good permeability, resistance is little, and beneficially bacterium cell contacts with the sulfur-containing foul material in waste gas, carries
High mass transfer efficiency.
5., for the carrier material light weight of microbial activity filler, apply the waste gas at purifying sulfur-containing foul material raw
In thing reactor, packed density is low, and the pressure loss is little.
6. the most perishable deformation of microbial activity filler, can be with life-time service and the actual bar adapting to Various Complex
Part.
Accompanying drawing illustrates:
Fig. 1 human pallid bacillus (Ochrobactrum anthropi) SL1 phylogenetic tree
Detailed description of the invention:
Example 1: a kind of microbial activity filler removing sulfur-containing foul material and preparation method thereof.
A kind of microbial activity filler removing sulfur-containing foul material, described filler appendix has human pallid bacillus
(Ochrobactrum anthropi) SL1, described human pallid bacillus (Ochrobactrum anthropi) SL1 protect
Hiding numbered CGMCC No.7400, described filler is polyurethane foamed blocks；
Described packing density is 0.9kg/m3, porosity is 86%, and particle diameter is 30mm；
Described microorganism with the form appendix of bacteria suspension on filler；
Described microbial bacteria suspension is 100: 10 with the mass ratio of light porous material；
Human pallid bacillus (Ochrobactrum anthropi) SL1 cell concentration in described microbial activity filler
More than 106CFU/g
Described microbial activity filler preparation process is as follows:
(1) slant culture: prepare human pallid bacillus (Ochrobactrum anthropi) SL1 slant strains；
Described human pallid bacillus (Ochrobactrum anthropi) SL1 slant medium consists of: glucose
0.5g, KH2PO42.0g, K2HPO42.0g, KNO32.0g, NH4Cl0.5g, MgCl2·6H2O0.2g,
FeSO4·7H2O0.01g, Na2S2O3·5H2O5.0g, agar 20g, water 1000ml, pH value 6.8-7.2,
121 DEG C of sterilizing 20min；
(2) preparation of bacteria suspension: human pallid bacillus (Ochrobactrum anthropi) SL1 inclined-plane thalline 10%
Inoculum concentration fermentation culture step by step, separating and obtaining cell concentration is 0.6g/L human pallid bacillus (Ochrobactrum
Anthropi) SL1 bacteria suspension；
Human pallid bacillus (Ochrobactrum anthropi) SL1 bacteria suspension preparation method is as follows；
Human pallid bacillus (Ochrobactrum anthropi) SL1 inclined-plane thalline ferments training step by step by 10% inoculum concentration
Support, 36 DEG C of incubation time 48h of cultivation temperature, ventilation 100L/min, dissolved oxygen ＞ 2.0mg/L, tank pressure
0.06Mpa, fermentation liquid is placed in refrigerated centrifuger in 4 DEG C, and 5000rpm, centrifugal concentrating 10min discard
Clear liquid, precipitation dilutes with sterile nutrient solution respectively, and obtaining cell concentration respectively is 0.5g/L human pallid bacillus
(Ochrobactrum anthropi) SL1 bacteria suspension；
Consisting of of described human pallid bacillus (Ochrobactrum anthropi) SL1 liquid fermentation medium:
Glucose 0.5g, KH2PO42.0g, K2HPO42.0g, KNO32.0g, NH4Cl0.5g, MgCl2·6H2O
0.2g, FeSO4·7H2O0.01g, Na2S2O3·5H2O5.0g, water 1000ml, pH value 6.8-7.2,121 DEG C
Sterilizing 20min；
Consisting of of described human pallid bacillus (Ochrobactrum anthropi) .SL1 sterile nutrient solution: Fructus Vitis viniferae
Sugar 0.5g, KH2PO43.0g, K2HPO43.0g, NH4 H2PO40.5g, MgCl2 6H2O0.2g, FeCl3
0.01g, water 1000ml, pH value 6.8-7.2；121 DEG C of sterilizing 20min；
(3) preparation of microbial bacteria suspension: human pallid bacillus (Ochrobactrum anthropi) SL1 bacterium is hanged
In liquid, thalline total concentration is 0.5g/L；
(4) preparation of microbial activity filler: the microbial bacteria suspension that step (3) is prepared by (a) passes through pump and spray
Drench the surface that head sprays the light porous material of packet or grain shape；Spray rate is 10L/min, spray
The pouring time is 60min；Being stirred, mixing speed is 100rpm simultaneously, and mixing time is 100min；Make
Bacteria suspension is fully contacted with light porous material；B () will be with human pallid bacillus (Ochrobactrum anthropi)
The porous material block (or granule) of SL1 is put in impeller, rotates mixing 50min under 10rpm rotating speed；
Along with the rotation of blender, it is attached to the bacterium cell on light porous material surface and enters into due to the effect of centrifugal force
Pore interior；C () to light porous material surface spraying microbial bacteria suspension, is stirred again simultaneously；Obtain
Obtaining microbial activity filler, 4 DEG C save backup；
Preferably repeat step (a)-(c) 3 times, make equal appendix in material surface and micropore have bacterium cell, it is thus achieved that
Microbial activity filler；
Described rotary blender is the revolution of efficient fine container, agitating type mixing apparatus, doing for different proportion
Property or the uniform mixing of moist particulate matter, and in mixed process, do not produce the melting of material, volatilize or rotten.
Rotary blender main body is that stainless steel material makes, and volume is 0.05-10m3, inside and outside wall polishes, without dead angle,
Built-in stirring vane, external rotating stirs plus built-in blade, and light porous filler and bacteria suspension are rotating mixing
Radial roller in device, the most also can horizontal and upper and lower convection current, mixing efficiency is high.In mixer rotary course,
On the one hand making each component substances be sufficiently mixed, on the other hand, due to the effect of centrifugal force, bacterium cell can enter
In hole within porous material, the bacterium cell appendix amount not only making microbial activity filler is high, and bacterium cell is not
It is easy to run off.
Example 2: a kind of microbial activity filler is at degraded H2Application in S gas.
It is another object of the present invention to utilize microbial activity filler to carry out odorous gas process, particularly in degraded
H2Application in S gas, specifically comprises the following steps that
(1) the microbial activity filler that appendix has human pallid bacillus (Ochrobactrum anthropi) SL1 is put
In a container, it is passed through H2S gas, with H2S gas is sulfur source, activates microbial activity filler；Activation
Time is 7 days；
Described H2The concentration of S is 35ppm；
(2) the microbial activity filler after activation is placed in bioreactor, is passed through the gas of sulfur-containing foul material
Body, purifies the sulfur-containing foul material in waste gas with human pallid bacillus (Ochrobactrum anthropi) SL1, micro-
Biological active filling material volume is 100m3, the total concentration of sulfide is 100ppm, and gas flow rate is 1000m3/ h,
Purification temperature is 25 DEG C；Periodically maintain microbial activity filler to microbial activity filling surface spraying nutrient solution
Activity, spray frequency be 3 days spray 1 time, each spray rate is 50L/min, and each spray time is
60min；
Consisting of of described nutritional solution: glucose 0.5g, KH2PO42.0g, K2HPO42.0g, KNO3
2.0g, NH4Cl0.5g, MgCl2·6H2O0.2g, FeCl30.01g, water 1000ml, pH6.8-7.2；With
The degradation capability of track detection sulfur-containing foul material.
Example 3: using effect experiment (example 1 filler is used for the process of following gas).
(1) processing the gases containing stench sulphureous gases of sewage plant sludge sedimentation tank loss, gas flow is 35m3/ h,
The time of staying is 1～3mins, operates under room temperature condition.The inlet gas concentration of hydrogen sulfide is: 14.86mg/m3,
Concentration of giving vent to anger is less than 0.03mg/m3, less than the emission standard for odor pollutants of country's formulation, clearance is higher than
99%.
(2) for processing the gases containing stench sulphureous gases that sludge composting produces, the concentration of hydrogen sulfide and ethyl mercaptan is respectively
For 21.2mg/m3And 3.21mg/m3, gas flow is 70m3/ h, the time of staying is 1～3mins, room temperature
Under the conditions of operate, the clearance of hydrogen sulfide and ethyl mercaptan is all higher than 90%.
(3) refuse landfill, processes and contains 162.1mg/m3 hydrogen sulfide, 3.82mg/m3 methanthiol, and
The mix waste gas of 8.2mg/m3 methyl sulfide, gas flow is 60m3/h, and the time of staying is 1～3mins, temperature
18-37 DEG C, hydrogen sulfide, methanthiol, the clearance of methyl sulfide respectively reach 90%, 86% and 88%.
Claims (3)
1. the human pallid bacillus removing sulfur-containing foul material (Ochrobactrum anthropi) SL1, it is characterised in that described human pallid bacillus (Ochrobactrum anthropi) SL1, deposit number is CGMCC No.7400.
The human pallid bacillus of removing sulfur-containing foul material the most according to claim 1 (Ochrobactrum anthropi) SL1 application in processing sulfur-containing foul material, it is characterised in that described sulfur-containing foul material is hydrogen sulfide, methanthiol, ethyl mercaptan, methyl sulfide.
The method that the most according to claim 1, the active filler prepared by human pallid bacillus carries out odorous gas process, step is as follows:
(1) appendix is had human pallid bacillus (Ochrobactrum anthropi) the human pallid bacillus active filler of SL1 is placed in a container, is passed through H2S gas, with H2S gas is sulfur source, activates human pallid bacillus active filler；Soak time is 1-14 days；
Described H2The concentration of S is 50-200ppm；
(2) will activation after human pallid bacillus active filler be placed in bioreactor, be passed through the gas of sulfur-containing foul material, with human pallid bacillus (Ochrobactrum anthropi) SL1 purifies the sulfur-containing foul material in waste gas, human pallid bacillus active filler volume is 0.001-200m3, the total concentration of sulfide is 50-200ppm, and gas flow rate is 0.05-10000m3/ h, purification temperature is 10-35 DEG C；Periodically maintaining the activity of active filler, spray frequency to human pallid bacillus active filler surface spraying nutritional solution is to spray 1 time for 1-7 days, and each spray rate is 1.0-200L/min, and each spray time is 20-120min；
Described sulfur-containing foul material is hydrogen sulfide, methanthiol, ethyl mercaptan, methyl sulfide；
Consisting of of described nutritional solution: glucose 0.5g, KH2PO42.0g, K2HPO42.0g, KNO32.0g, NH4Cl 0.5g, MgCl2·6H2O 0.2g, FeCl30.01g, water 1000ml, pH 6.8-7.2.
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| 一株高效脱硫菌的筛选及脱硫性能的研究;孟建宇，张守城;《内蒙古大学学报》;20120131;69-72 * |
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