CN104593287B - A kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production - Google Patents
A kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production Download PDFInfo
- Publication number
- CN104593287B CN104593287B CN201410774486.4A CN201410774486A CN104593287B CN 104593287 B CN104593287 B CN 104593287B CN 201410774486 A CN201410774486 A CN 201410774486A CN 104593287 B CN104593287 B CN 104593287B
- Authority
- CN
- China
- Prior art keywords
- fluoxastrobin
- preparation
- application
- bacterial strain
- anthropi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Biomedical Technology (AREA)
- Hydrology & Water Resources (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production.The bacterial strain be anthropi (Ochrobactrum anthropi) SH14, the bacterial strain is preserved in China typical culture collection center on December 20th, 2013（CCTCC）, preserving number is CCTCC NO：M 2013681.The bacterial strain can effectively degrade Fluoxastrobin residues of pesticides in a short time, available for natural environments such as water body, the soil for repairing Fluoxastrobin pollution.The preparation being prepared with the bacterial strain, there is the advantages that production cost is low, easy to use, removal effect is good.Using this microbial inoculum the residual quantity of Fluoxastrobin in water body and soil can be made to reduce by more than 85% in a short time, effectively repair the natural environment of Fluoxastrobin pollution, solve agricultural production Pesticide Residues excessive problem and problem of environmental pollution, preserved the ecological environment and human health.
Description
Technical field
The present invention relates to application environment microorganism and agriculture field, and in particular to a kind of Fluoxastrobin degradation bacteria and its production
The natural environment such as microbial inoculum and application, water body or soil specifically for repairing Fluoxastrobin pollution, preserves the ecological environment and is good for the mankind
Health.
Background technology
Fluoxastrobin, also known as Amici reach, and are a kind of the most frequently used methoxy acrylic bactericides.Fluoxastrobin is 1996
Year starts to list and put into agricultural production application, is stepped in global 72 countries, 80 various crop diseases at present
Note uses.The bactericide possesses the complete super wide fungicidal spectrum not having, to exhausted in sac fungus, basidiomycetes, Fungi Imperfecti and Oomycete
Most of disease fungus has preferable bacteriostatic activity, has been widely used for preventing and treating crop disease in recent years, is the master of in the market
Want bactericide kind.
With the extension for the increasingly extensive and usage time that Fluoxastrobin uses, it is in addition fast light, hot the characteristics of, it is in soil
Degradation half life be up to 56~279 days, therefore the residue problem about the agricultural chemicals and its endanger getting worse.Numerous studies table
Bright, Fluoxastrobin increasingly highlights to the harmfulness of non-target organism, such as causes high risks to some beneficial insects such as honeybee,
There is very high toxicity to fish and aquatic invertebrate.In addition, the bactericide has an environmental hormone effects, Long Term Contact or
Intake will cause chronic disease, have chronic toxicity to the mankind, birds and other mammals.Therefore, phonetic bacterium in environment is eliminated
The residual contamination of ester has turned into the researcher scientific research proposition with great economy and social effect urgently to be resolved hurrily.
Biological prosthetic (Bioremediation) technology because of its easy to operate, economical and practical, advantages of environment protection, into
To handle the effective measures of all kinds of environmental contaminants.The elder generation of oil pollution is successfully administered using bioremediation technology in the world
Example.In recent years, research and utilization bioremediation technology administers pesticide residual contamination turns into study hotspot.Current many developed countries
Huge fund is all put into major company to be engaged in the research and development of residues of pesticides biodegradable formulations, such as BCI companies of the U.S., U.S.'s work
Journey services biological prosthetic company by formulation products large-scale production of degrading, the domestic limited public affairs of Beijing Jia Nongxin Trade Developments
Successfully production " than sub- " biodegradable formulations can be used for removing organophosphorus pesticide residue for department.But because pesticide structure is more
Sample, and Degradation of Pesticides By Microorganisms often has very strong specific aim, cause existing degradation bacteria resources bank far from meeting agricultural chemicals
The biological prosthetic actual demand of residual contamination.There is presently no the degrading microorganism and its related degradation system specifically for Fluoxastrobin
Agent product.
The content of the invention
It is an object of the invention to the above-mentioned deficiency for prior art, there is provided the anthropi of one plant of degraded Fluoxastrobin
(Ochrobactrum anthropi)SH14。
It is a further object of the present invention to provide application of the bacterial strain in Fluoxastrobin residues of pesticides of degrading.
It is a further object of the present invention to provide application of the bacterial strain in the preparation for preparing degraded Fluoxastrobin.
It is a further object of the present invention to provide a kind of preparation for Fluoxastrobin of degrading.
It is a further object of the present invention to provide preparation the answering in Fluoxastrobin residues of pesticides of degrading of the degraded Fluoxastrobin
With.
The above-mentioned purpose of the present invention is achieved by the following technical programs：
Anthropi (Ochrobactrum anthropi) SH14 of one plant of degraded Fluoxastrobin, the fungi preservation is in
State's Type Tissue Collection (CCTCC), address are Wuhan, China, and Wuhan University, preserving number is CCTCC NO：M
2013681, preservation date is on December 20th, 2013.
The bacterial strain is through morphological feature, physio-biochemical characteristics, 16S rDNA Phylogenetic Analysis and API 20NE systems
Be accredited as anthropi (Ochrobactrum anthropi), the bacterial strain grows quickly in LB solid plates, present white or
Faint yellow, bacterium colony is small and flat, slightly transparent, neat in edge, surface relatively moistening.Determined by physio-biochemical characteristics, show the bacterial strain
Belong to gram-Negative bacillus, aerobic, catalase, oxidase positive.The bacterium can utilize glucose, arabinose, mannose, the last of the ten Heavenly stems
Acid, citric acid, maltose, apple acid, potassium nitrate and N-ACETYL-D-GLUCOSAMINE；Do not utilize arginine, gelatin, aesculin, oneself
Diacid, mannitol, urea element, tryptophan, gluconate, phenylacetic acid and to nitro-β-D- methylgalactoses.
A kind of preparation for Fluoxastrobin of degrading, the preparation are prepared by following methods：
S1. the bacterial strain of the anthropi SH14 is cultivated in seed culture medium, obtains strain；
S2. above-mentioned strain is inoculated with into containing seed by 5%~10% inoculum concentration of the volume ratio of seed tank culture base
The seeding tank of culture medium, cultivate to exponential phase, obtain seed liquor；
S3. the seed liquor of acquisition is inoculated with according to 5%~10% inoculum concentration and fermented into the production containing fermentation medium
Tank fermented and cultured, go out tank and form liquid bacterial agent；
In the incubation of seeding tank and production fermentation tank, the throughput of filtrated air is 0.45~0.60m3/ min,
Mixing speed is 200~250r/min, 30 DEG C of temperature, and whole process incubation time is about 48h, thalline quantity after fermentation ends >=
1.0×109CFU/mL, nutrient solution goes out tank and is directly distributed into preparation with plastic barrel or Packaging Bottle after the completion of fermentation；
Wherein, described seed culture medium, the culture medium used in seeding tank, the culture medium used in production fermentation tank are identical,
Formula is：Yeast extract 0.5%, peptone 1%, NaCl 1%, pH value 7.5.
Sprayed application to after described anthropi SH14 preparation diluents in crops, soil or water body, the liquid after dilution
Thalline quantity is at least up to 1.0 × 10 in microbial inoculum7CFU/mL。
Applications of the described anthropi SH14 in Fluoxastrobin of degrading.
The application of described anthropi SH14 preparations Fluoxastrobin in degraded water body, soil.
Beneficial effects of the present invention are as follows：
Present invention screening obtains one plant of anthropi (Ochrobactrum anthropi) SH14, and the bacterial strain can be in short-term
Interior effectively degraded Fluoxastrobin residues of pesticides, available for natural environments such as water body, the soil for repairing Fluoxastrobin pollution, solve agricultural
Pesticide Residues excessive problem and problem of environmental pollution are produced, is preserved the ecological environment and human health.
Invention further provides the liquid bacterial agent being prepared using anthropi SH14 productions, there is production cost
It is low, easy to use, the advantages that removal effect is good, it is adapted to administer pollution caused by Fluoxastrobin in the environment such as water body and soil, has
Very important theoretical and application value.
Brief description of the drawings
Fig. 1 anthropis SH14 degraded Fluoxastrobins produce transparent circle.
Fig. 2 anthropis SH14 16S rDNA phylogenetic analysis.
The HPLC chromatogram of Fig. 3 anthropis SH14 degraded 50mg/L Fluoxastrobins.
The HPLC chromatogram (50mg/L) of Fig. 4 Fluoxastrobin standard items.
Embodiment
The present invention is further elaborated on reference to Figure of description and specific embodiment.Following examples are this hair
Bright preferable embodiment, but protection scope of the present invention is not limited in any form.Described in main elaboration of the invention
Bacterial strain and the application thought based on the bacterial strain, in embodiment the replacement of simple parameter can not be gone to live in the household of one's in-laws on getting married one by one in embodiment
State, but and be not so limited the present invention, the change made under other any Spirit Essences and principle without departing from the present invention, repair
Decorations, substitute, combine, simplifying, equivalent substitute mode should be considered as, should all include within the scope of the present invention.
Unless stated otherwise, the reagent of the invention used, method and apparatus for the art conventional reagent, method and are set
It is standby.Unless stated otherwise, agents useful for same and material of the present invention are purchased in market.
The anthropi SH14 of embodiment 1 separation and identification
The present inventor is separated by enrichment culture method, screens Fluoxastrobin degradation bacteria.For being enriched with the environmental sample of Fluoxastrobin
Gather from the activated sludge in Singapore's treatment tank.Taking activated sludge sample 5g to add 50mL basal mediums, (formula is：
(NH4)2SO4, 2g；MgSO4,0.2g；CaCl2, 0.01g；FeSO4, 0.005g；MnCl2, 0.002g；K2HPO4, 10.5g；
KH2PO4, 4.5g, distilled water 1000mL, pH 7.5) in.Fluoxastrobin active compound mother liquor (DMSO is solvent) is added simultaneously, makes Fluoxastrobin
Final mass concentration is 50mg/L, and degrading microorganism enrichment culture is carried out under 30 DEG C, 150r/min shaking tables.After cultivating 7d, press
10% inoculum concentration is transferred in the basal medium of second batch Fluoxastrobin containing 100mg/L.After the same terms culture 7d, then press
In the basal medium that it is 200mg/L containing Fluoxastrobin that 10% inoculum concentration, which is transferred to, continue to cultivate 7d.By that analogy, constantly increase
Add the mass concentration of Fluoxastrobin.0.2mL basal mediums zymotic fluid is finally taken uniformly to be applied in the solid plate containing Fluoxastrobin
Line separation is repeated, until obtaining single bacterium colony, and its degradation effect is verified using high performance liquid chromatography (HPLC).
HPLC condition determinations are as follows：
HPLC models：2690 types (Waters, USA)；Chromatographic column：C18Reversed-phase column (Phenomenex, 250nm × 4.60mm,
5μm)；Flow velocity：1mL/min；Column temperature：Normal temperature (28 ± 1 DEG C)；Mobile phase：Acetonitrile: water=70: 30 (containing 0.1% acetic acid)；Detection
Wavelength：220nm；Sample size：10μL.Under these conditions, Fluoxastrobin peak shape is made up of a peak, and peak is sharply, stably, retains
Time is 5.677min (see Fig. 4).
Degradation rate computational methods are as follows：
It is successfully separated using the above method from environmental sample and obtains one plant of Fluoxastrobin degradation bacteria, numbering SH14, the bacterium
Strain can be grown by the use of Fluoxastrobin as sole carbon source, reach more than 85% to the degradation rate of 50mg/L Fluoxastrobins in 5d.Bacterial strain
SH14 grown in LB solid plates quickly, present it is white or faint yellow, bacterium colony is small and flat, slightly transparent, neat in edge, surface compared with
Moistening is (see Fig. 1).Determined by physio-biochemical characteristics, show that the bacterial strain belongs to gram-Negative bacillus, aerobic, catalase, oxygen
Change enzyme positive.The bacterium can utilize glucose, arabinose, mannose, capric acid, citric acid, maltose, apple acid, potassium nitrate and
N-ACETYL-D-GLUCOSAMINE；Arginine, gelatin, aesculin, adipic acid, mannitol, urea element, tryptophan, gluconic acid are not utilized
Salt, phenylacetic acid and to nitro-β-D- methylgalactoses.Further design PCR primer is identified by 16S rDNA, the strain gene
(GenBank accession number is sequence with the Ochrobactrum anthropiCCUG 34735 in BLAST online databases：
AM114407) similitude reaches more than 96%.By with related strain 16s rDNA phylogenetic analysis, bacterial strain SH14 is preliminary
It is accredited as Ochrobactrum anthropi.Pass through French Mei Liai identification systems API 20NE (bioM é rieux, France)
Further identification is done, as a result shows bacterial strain SH14 and Ochrobactrum anthropi similarities up to 99.9% (see Fig. 2).Cause
This, is anthropi through morphological feature, physio-biochemical characteristics, 16S rDNA Phylogenetic Analysis and API 20NE system identifications
(Ochrobactrum anthropi).Anthropi SH14 was stored in China typical culture collection on December 20th, 2013
Center (CCTCC), address are Wuhan, China, and Wuhan University, preserving number is CCTCC NO：M 2013681.
The preparation of the anthropi SH14 liquid preparations of embodiment 2
It is using the above-mentioned anthropi SH14 technological process of productions for preparing liquid preparation：Inclined-plane kind-shake-flask seed liquid-kind
Sub- tank culture-production tank ferments-is packaged as liquid bacterial agent.
Anthropi SH14 strain is activated on LB solid plates, is inoculated in standby on test tube slant.By pale bar
Bacterium SH14 test tube kind is inoculated in the LB culture medium 1000mL shaking flasks containing 250mL, and the LB culture medium prescriptions are：Yeast extract
0.5%, peptone 1%, NaCl 1%, pH value 7.5.30 DEG C of constant temperature oscillations prepare inoculation seeding tank, seeding tank to logarithmic phase
Liquid amount is 70%.In 1.1Kg/cm after the completion of feeding intake3Pressure under, high pressure moist heat sterilization under the conditions of 121 DEG C, be cooled to 30 DEG C
Afterwards, above-mentioned cultured shaking flask strain is inoculated in the seeding tank that liquid amount is 70% by 10% inoculum concentration, filtrated air
Throughput is 0.45~0.60m3/ min, mixing speed 225r/min, cultivate standby to exponential phase.Logarithmic phase will be reached
Seed liquor according to 10% inoculum concentration input liquid amount be 70% production tank fermented and cultured.Production tank after feeding intake,
1.1Kg/cm3Pressure under, high pressure moist heat sterilization under the conditions of 121 DEG C, after being cooled to 30 DEG C, lead to filtrated air, throughput is
0.45~0.60m3/ min, mixing speed 225r/min, cultivation temperature control is 30 DEG C, and whole technique culture flow time is about
For 48h, thalline quantity >=1.0 × 10 after fermentation ends9CFU/mL, nutrient solution goes out tank and directly uses plastic barrel after the completion of fermentation
Or Packaging Bottle is distributed into preparation.
Wherein, described seed culture medium, the culture medium used in seeding tank, the culture medium used in production fermentation tank are identical,
Formula is：Yeast extract 0.5%, peptone 1%, NaCl 1%, pH value 7.5.
Biodegradable effects of the anthropi SH14 of embodiment 3 to Fluoxastrobin
Fluoxastrobin is added in basal medium (with embodiment 1), makes its final concentration of 50mg/L；Inoculum density is 1.0
×107CFU/mL microbial inoculum, and the culture medium for not connecing bacterium is set as control, 30 DEG C of 0~5d of culture, periodically sampling, using HPLC methods
Determine the residual quantity of agricultural chemicals and calculate degradation rate, the results are shown in Table shown in 1 and Fig. 3.As a result show, anthropi SH14 is in the short time
The interior Fluoxastrobin that can effectively degrade, the interior degradation rates to Fluoxastrobin of 3d reach in 76.8%, 5d to be reached to the degradation rate of Fluoxastrobin
86.3%, there is preferable biodegradable effect.
Degradation effects of the anthropi SH14 of table 1 to Fluoxastrobin
Claims (7)
1. one plant degraded Fluoxastrobin anthropi (Ochrobactrum anthropi) SH14, it is characterised in that the bacterium
Strain is preserved in China typical culture collection center on December 27th, 2013（CCTCC）, preserving number is CCTCC M
2013681。
2. application of the bacterial strain described in claim 1 in Fluoxastrobin residues of pesticides of degrading.
3. application of the bacterial strain described in claim 1 in the preparation for preparing degraded Fluoxastrobin.
4. a kind of preparation for Fluoxastrobin of degrading, it is characterised in that the preparation is prepared by following methods：
S1. the bacterial strain of anthropi SH14 described in claim 1 is cultivated in seed culture medium, obtains strain；
S2. above-mentioned strain is inoculated with into containing seed culture medium by 5%~10% inoculum concentration of the volume ratio of seed culture medium
Seeding tank, cultivate to exponential phase, obtain seed liquor；
S3. the seed liquor of acquisition is inoculated with according to 5~10% inoculum concentration and trained into the production ferment tank containing fermentation medium
Support, go out tank and form liquid bacterial agent；
In the incubation of seeding tank and production fermentation tank, the throughput of filtrated air is 0.45~0.60 m3/ min, stirring
Speed is 200~250 r/min, 30 DEG C of temperature, and whole process incubation time is 48 h, thalline quantity >=1.0 after fermentation ends ×
109CFU/mL；
Wherein, described seed culture medium, fermentation medium are identical, and formula is：Yeast extract 0.5%, peptone 1%, NaCl
1%, pH value 7.5.
5. application of the preparation in Fluoxastrobin residues of pesticides of degrading described in claim 4.
6. application according to claim 5, it is characterised in that the preparation described in claim 4 is in degraded water body or soil
The application of middle Fluoxastrobin agricultural chemicals.
7. application according to claim 6, it is characterised in that water will be sprayed onto after the preparation diluent described in claim 4
In body or soil, the quantity of thalline is at least up to 1.0 × 10 in the preparation after dilution7 CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410774486.4A CN104593287B (en) | 2014-12-16 | 2014-12-16 | A kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410774486.4A CN104593287B (en) | 2014-12-16 | 2014-12-16 | A kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104593287A CN104593287A (en) | 2015-05-06 |
| CN104593287B true CN104593287B (en) | 2018-03-06 |
Family
ID=53119345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410774486.4A Active CN104593287B (en) | 2014-12-16 | 2014-12-16 | A kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104593287B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110255717A (en) * | 2019-06-06 | 2019-09-20 | 华南农业大学 | A kind of degradation bacteria and its application of methoxy acrylic bactericide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294140A (en) * | 2007-12-26 | 2008-10-29 | 广东省微生物研究所 | Guangzhou pallor bacillus with high-efficiency degradation ability for pyridine |
| CN103266075A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 and application thereof to removal of repugnant substances containing sulfur |
| CN103937722A (en) * | 2014-04-24 | 2014-07-23 | 烟台海上传奇生物科技有限公司 | Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent |
-
2014
- 2014-12-16 CN CN201410774486.4A patent/CN104593287B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294140A (en) * | 2007-12-26 | 2008-10-29 | 广东省微生物研究所 | Guangzhou pallor bacillus with high-efficiency degradation ability for pyridine |
| CN103266075A (en) * | 2013-05-29 | 2013-08-28 | 中国科学院生态环境研究中心 | Ochrobactrum anthropi SL1 and application thereof to removal of repugnant substances containing sulfur |
| CN103937722A (en) * | 2014-04-24 | 2014-07-23 | 烟台海上传奇生物科技有限公司 | Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent |
Non-Patent Citations (1)
| Title |
|---|
| 苍白杆菌B2对甲基对硫磷降解途径研究;柏文琴 等;《农药学学报》;20041231;第6卷(第4期);48-54 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110255717A (en) * | 2019-06-06 | 2019-09-20 | 华南农业大学 | A kind of degradation bacteria and its application of methoxy acrylic bactericide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104593287A (en) | 2015-05-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102757915B (en) | Chloro acetamide herbicide degrading bacteria as well as bactericide prepared thereby and application thereof | |
| CN106135285B (en) | Application of the Rhodopseudomonas palustris biocontrol agent in prevention and control rice blast | |
| CN107058186B (en) | A kind of pyrethroid insecticides residue degrading bacterial strain and its application | |
| CN102604859B (en) | Pyrethroid pesticide residue degradation bacteria as well as bacteria agent and application | |
| CN108998394A (en) | A kind of bacillus and its application | |
| Chhetri et al. | Chryseobacterium tagetis sp. nov., a plant growth promoting bacterium with an antimicrobial activity isolated from the roots of medicinal plant (Tagetes patula) | |
| TWI233944B (en) | Chlorophyll-rich and salt-tolerant chlorella | |
| CN104726378A (en) | Method for improving protective enzyme activities of salt-stressed turfgrass by adopting enhanced salt-tolerant microbial agent | |
| CN106085899B (en) | Microbial inoculum and the application of one plant of process for preparation of benzoylurea compounds degradation bacteria and its production | |
| Lipková et al. | Growth promotion of rapeseed (Brassica napus L.) and blackleg disease (Leptosphaeria maculans) suppression mediated by endophytic bacteria | |
| Chebotar et al. | Endophytes from halotolerant plants aimed to overcome salinity and draught | |
| CN107541479A (en) | A kind of insecticide d-phenothrin degradation bacteria strains and its microbial inoculum and degradation technique | |
| Wang et al. | Progress of euhalophyte adaptation to arid areas to remediate salinized soil | |
| Wang et al. | Research progress regarding the role of halophilic and halotolerant microorganisms in the eco-environmental sustainability and conservation | |
| CN105002110B (en) | Complex microorganism preparations and its application in the processing of algal bloom water body | |
| CN100415872C (en) | DDT pesticide residue degradation bacterium and produced bacterium agent | |
| CN104480043B (en) | Rhodococcus sp. capable of degrading four herbicides and application thereof | |
| CN103966138B (en) | The secondary coccus N74-1 of Bangladesh and the application in aquaculture water purifying thereof | |
| CN104593287B (en) | A kind of Fluoxastrobin degradation bacteria and its microbial inoculum and the application of production | |
| Zhang et al. | The prevention of bio-organic fertilizer fermented from cow manure compost by Bacillus sp. XG-1 on watermelon continuous cropping barrier | |
| CN106591168B (en) | A kind of nicosulfuron degradation Rhodococcus ruber YMHL-1 and its application | |
| CN105154365B (en) | Mycobacteria YC-RL4 and its application | |
| CN104673704B (en) | The pseudomonas aeruginosa strains of one plant of degraded agricultural chemicals containing ester bond | |
| CN103911319B (en) | Pyrethrin degradation bacteria strains and microbial inoculum thereof and application | |
| CN104789507A (en) | Bacteria BMJHZ-01 for degrading difenoconazole and screening method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |