KR101106035B1 - Bacillus firmus bc9 10865p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it - Google Patents

Abstract

The present invention relates to a odor removal and heavy metal removal composition comprising the new strain Bacillus permus BC9 KCCM 10865P and a method for purifying livestock wastewater or food waste using the same, and more particularly, the new strain Bacillus permus BC9 KCCM 10865P or an active ingredient thereof. It relates to a microbial agent containing and a method for purifying livestock wastewater or food waste using the same.

Description

BACILLUS FIRMUS BC9 10865P REDUCING NASTY ODOR AND HEAVY METAL AND CLARIFICATION METHODS FOR RESOURCE RECOVERY FROM FOOD WASTE WATERSTOCK USING IT}

An object of the present invention is to provide a microorganism which reduces odor and heavy metals of livestock wastewater and food waste.

It is also an object of the present invention to provide a method for producing organic liquid fertilizer using livestock wastewater and a livestock feed production method using food waste.

The present invention relates to a microorganism for reducing odor generated from wastewater or food waste generated in livestock raising and heavy metals in the wastewater and garbage, and to a method for purifying livestock wastewater or food waste using the same.

Odors generated during livestock manure storage in the pit of the barn, and the resulting odors, which are harmful to both livestock and humans, are mostly global warming gases. It is a substance. These odors are mainly derived from livestock stocks, and their general composition shows that they are easily decomposed by microorganisms such as carbohydrates, starches, proteins and cellulose, and eggs that are not easily degraded by microorganisms such as fats and lignin. It can be divided into degradable substances.

In addition, the odor components generated when the nutrient is decomposed in the aerobic state are ammonia, methylmecaptan, hydrogen sulfide, methyl sulfide, methyl disulfide, trimethylamine, acetaldehyde, styrene, propionic acid, butyric acid and gil acetic acid. Odors come in many forms. For example, methylmecaptan is a rotten onion smell, hydrogen sulfide is a colorless gas, irritating, suffocating and rotting onion, and ammonia is a colorless gas that is irritating and has a characteristic odor like urine. If you do so, you will get a disgusting hygiene and inhibit livestock growth.

Odor discomfort continues to increase at present, and legal regulations have been enacted and enforced to raise the importance of odor management. Odors are reported as livestock wastewater treatment plants, food waste treatment plants, manure treatment plants, landfills, and feed manufacturing industries. However, the odor generated in such a facility is not generated only in some processes but is generated in the entire process, and there is a great difficulty in process management, facility improvement, and prevention facility installation to reduce odor occurrence. In addition, since such environmental basic facilities are mainly mixed with residential areas, it is necessary to develop highly efficient odor reduction technology to minimize the impact on the surroundings.

Legal regulations on odors currently include hydrogen sulfide (H ₂ S), methyl mercaptan (CH ₃ SH), methyl sulfide ((CH ₃ ) ₂ S), methyl disulfide ((CH ₃ ) ₂ S ₂ ), and ammonia (NH ₃ ), Trimethylamine ((CH ₃ ) ₃ N), acetaldehyde (CH ₃ CHO), styrene (C ₆ H ₅ CH = CH ₂ ) is carried out for eight items, these components are different from other organic compounds It is known to have a strong smell even at low concentrations. In particular, the odor generated in the barn such as pigs, barn, cages, etc., hydrogen sulfide (H ₂ S) is the main pollutant, but in addition, ammonia (NH ₃ ), methyl mercaptan (CH ₃ SH), trimethylamine ((CH ₃ ) ₃ N) and contains a substance such as a lower fatty acid to thereby increase the strength of odor.

In general, malodorous substances cause various malodorous compounds by their source, causing malodors. As a single compound, hydrogen sulfide (H ₂ S) smells like rotting eggs, and mercaptan smells like rotting vegetables. The amines have a peculiar smell like fish smell.

As a method of removing odors, there are largely methods of separating or destroying odorous substances, such as oxidation, enzymatic decomposition, and adsorption, and so-called masking, which simply hides odors. Oxidation has been suggested to oxidize and decompose odorous substances and to remove odors fundamentally with a wide range of bactericidal effects on bacteria, fungi and viruses. The types of oxidants that have been widely used in chemical oxidation methods include chlorine dioxide (duozone), sodium hypochlorite (Lax), and chlorine dioxide, but these substances contain chlorine and may have harmful effects on humans and livestock. . Enzymatic digestion is a deodorant made by extracting plant extracts, but it has a function of decomposing odors.However, specific components and principles of action are not known precisely.Adsorption is a method of adsorbing and removing malodorous substances by using an adsorbent having a large surface area such as activated carbon. When used for a certain time, there is a disadvantage that the efficiency decreases due to the rapid decrease of the surface area of the adsorbent. The masking method solves the sensory odor problem by dissolving natural or artificial flavors in a volatile solvent such as methyl alcohol and then volatilizing it in the air, but it is not a fundamental solution to the odor.

Although there is a method of using ozone or ultraviolet light alone or in combination as a method without using chemicals, ozone has a sterilizing and deodorizing effect, but excessive generation of ozone may act as a new pollutant. In addition, a method of decomposing odor sources using photocatalysts such as TiO ₂ with ozone generation has been proposed, but catalysts such as TiO ₂ have limitations in increasing the specific surface area, as well as hydrogen sulfide (H ₂ S) and methyl mercaptan (CH _3). When interacting with sulfides such as SH), methyl sulfide ((CH ₃ ) ₂ S), and methyl disulfide ((CH ₃ ) ₂ S ₂ ), the catalyst surface is easily poisoned and the catalyst efficiency decreases drastically. .

In general, odor, benzene, toluene, and xylene in various industrial sites such as petrochemical plant, urban sewage / manure treatment plant, rubber manufacturing plant, painting plant, food processing plant, livestock manure treatment plant, and manure treatment plant. Volatile organic compounds (VOCs) such as BTX, organic acids, aldehydes, ketones, and aromatic compounds are generated in large quantities at the same time, which causes delays in the construction of factories necessary for industrial development due to surrounding complaints. Construction of sewage treatment plants and landfills is delayed.

Removal of odors and volatile organic compounds (VOCs) generated in industrial sites can be largely divided into chemical and biological methods. The chemical method of combustion produces secondary air pollution, and the adsorption method is uneconomical due to the use of expensive activated carbon. These methods cost a lot of money due to excessive chemical consumption instead of low construction costs. On the other hand, biological methods have disadvantages in that construction costs and operating conditions are difficult, but they are easy to maintain and manage and consume less operating costs. Biological odor removal methods include biofilters filled in reactors by immobilizing malodor-decomposing microorganisms on a carrier, and biofilters have emerged as an odor treatment method that is economical and does not cause secondary pollution. The operating costs for operating the biofilter system are the most economical. Operation costs are high in order of biofilter, chemical liquid cleaning, catalytic combustion, activated carbon adsorption, and direct combustion.

Korean Patent Application No. 10-1996-0025606 (filed June 29, 1996) relates to a fermentation agent composition and a composting method for biological treatment of food wastes, high temperature resistant filamentous fungi; High temperature resistant and high temperature proteins belonging to Bacillus bacteria and oil-fat decomposition bacteria; Thermophilic fungi; Provided is a fermentation agent composition for fast fermentation treatment of food waste containing high temperature resistant actinomycetes adapted to high temperature.

Korean Patent No. 292879 (filed April 7, 1999) relates to a microbial preparation for condensation treatment and a method of treating condensate using the same, and has an excellent ability to secrete carbohydrates and protein processing enzymes of condensate (Bacillus subtilis ( Bacillus subtilis) subtilis KCTC 1028) and photosynthetic microorganisms (Rhodopseudomonas sp., a photosynthetic microorganism deposited as KCTC 8937P with the Korea Advanced Institute of Science and Technology Gene Bank) By applying microbial preparations to livestock and livestock farms, there is provided a method for treating livestock, which has the function of removing odors and consequently improving the livestock environment and livestock environment of livestock.

Korean Patent No. 378667 (filed Aug. 31, 2000) relates to a method for rapid deodorization and composting and composition of livestock manure, and is a decay odor generated in the early stages of decay such as odor or food waste generated from livestock manure. The present invention relates to a composition and a method for manufacturing the same, which are used to remove the organic matter and rapidly fermentatively decompose to form an odorless organic fertilizer. Streptomyces sp., Bacillus sp., Micrococcus sp., Achromobacter sp., Flavobacterium sp. One or more strains selected from the genus Escherichia sp. And Pseudomonas sp. Are aerobicly cultured in common liquid media such as neutrient or TSB media to select them from limestone powder, bentite clay or humus soil. An odorless composition obtained by adsorbing on an adsorption medium and then drying is provided.

Korean Patent No. 433268 (filed Oct. 5, 2000) relates to a fermentation agent composition for biological treatment of malodorous substances and a method for producing the same, and includes a high temperature organic substance and a high temperature resistant malodorous filamentous fungus; Filamentous fungi with consideration of substrate transition and excellent odor availability; Substrate degrading bacteria and odor useful bacteria belonging to Bacillus bacteria having high temperature and high temperature and broad substrate availability; It relates to a fermentation agent composition consisting of microorganisms for odor oil containing high temperature resistant actinomycetes adapted to high temperature while being adapted to high concentration organic matter and a method of manufacturing the same. The high temperature odor-resistant filamentous fungus is Aspergillus niger , Aspergillus oryzae , Penicillium spp., Mucor spp., Tricho At least one mixed bacterium selected from the group consisting of Trichoderma viride , Cephallosporium acremonium , and Issatchenkia orientalis is preferred, and the substrate transition relationship The filamentous fungi that are highly contemplated and odor-soluble are at least one selected from the group consisting of Candida spp., Arthrobacter spp., And Nocardia spp. Mixed bacteria were used, and the high temperature resistant, high temperature resistant, broad-substrate usable substrate degrading bacteria and odor useful bacteria belong to Bacillus subtilis. Odor -10 (Bacillus subtilis CH-10) , Bacillus mesen Terry carcass (Bacillus mesentericus), Bacillus mega emitter Solarium (Bacillus megaterium), Bacillus piece you miss formate enel alahyi -33 (Bacillus licheniformmis NLRI-33) , Bacillus turing if N-Sys ( Bacillus thuringiensis ), one or more mixed bacteria selected from the group consisting of Bacillus circulans was used, the thermophilic actinomycetes adaptable to the high concentration organic matter, Thermoactinomyces sp. And / or thermomonspora spp. In addition, the high temperature resistant actinomycetes adapted to the high temperature while being adaptable to the high concentration organic material are Streptomyces griseus , Streptomyces globisporius , Streptomyces flavus It is preferable that it is at least one mixed bacterium selected from the group consisting of, and the above-mentioned substrate degrading bacteria and odor useful bacteria having broad substrate availability are Pseudomonas putida NLRI-X50 and Corynebacterium. Corynebacterium spp., Staphylococcus lentus ( Staphylococcus lentus ), Micrococcus lylae ( Micrococcus lylae ) is one or more selected from the group consisting of mixed bacteria is said to be preferable.

Korean Patent Application No. 10-2005-0044120 (filed May 25, 2005) relates to an air purifier for removing odors and yellow dust in barns and has been applied to remove odors using a Bacillus subtilis.

Korean Patent Application No. 10-2006-0032543 (filed April 11, 2006) discloses a method for efficiently treating malodorous waste gas using a hybrid system process including a photocatalytic reactor, a fluidized bed aerobic system, and an anaerobic tank and a biofilter. The device has been filed.

Korean Patent Application No. 10-2004-0011349 (filed February 2, 2004) provides a biofilter to which the strain of the genus Geotricum is fixed and a method of removing methyl ethyl ketone using the same. When the methyl ethyl ketone was removed using a biofilter immobilized with a strain of Geotricum on the carrier, it was relatively inexpensive and did not generate nitrogen oxides or secondary pollutants compared to conventional methods such as activated carbon or incineration.

Korean Patent Application No. 10-2003-0078402 (filed Nov. 6, 2003) provides a biofilter for removing odors and volatile organic compounds, and removes volatile organic compounds using a strain of Pseudomonas genus. It has been shown.

Technical Challenge

It is an object of the present invention to provide a Bacillus firmus BC9 KCCM 10865P strain for reducing odor generated from wastewater or food waste generated in livestock raising and heavy metals in the wastewater and waste.

An object of the present invention is to provide a microbial agent for removing odor and heavy metals containing Bacillus firmus BC9 KCCM 10865P strain as an active ingredient and a method for purifying livestock wastewater or food waste using the same.

Technical solution

The present invention provides a Bacillus firmus BC9 KCCM 10865P strain that has the ability to remove odors much faster and more efficiently than conventional strains, and also has the ability to remove heavy metals.

The present invention provides a microbial agent for odor removal and heavy metal removal containing Bacillus firmus BC9 KCCM 10865P strain as an active ingredient.

Bacillus firmus BC9 (Cacillus firmus BC9) KCCM 10865P strain according to the present invention excellently reduced the odor generated in livestock wastewater or food waste. In particular, the microorganisms also reduced heavy metals from livestock wastewater or food waste. Therefore, the microbial agent according to the present invention can be widely used for purifying odors and heavy metals of livestock wastewater or food waste. In addition, it can be widely used for the production of organic liquid fertilizer using livestock wastewater or livestock feed using food waste.

1 shows an experimental apparatus for removing odor of livestock wastewater.

Figure 2 shows an experimental device for removing odor of food waste.

Best Mode for Carrying Out the Invention

The present invention provides a strain of Bacillus firmus BC9 KCCM 10865P, which is useful for removing odors and removing heavy metals.

Another aspect of the present invention provides a microbial agent for odor removal and heavy metal removal comprising an effective amount of the new strain Bacillus permus BC9 KCCM 10865P.

The method for formulating Bacillus permus BC9 KCCM 10865P according to the present invention can apply conventional techniques. Methods for formulating microorganisms include a method of preparing a solid in the form of beads and a method of preparing a liquid formulation. In the case of preparing the microorganism in a solid form, the product may be non-uniform in the manufacturing process, there is a disadvantage such as reduced application of the product due to the deactivation of microorganisms or dust generated in the manufacturing process. On the other hand, the liquid microbial agent has advantages such as faster activity and easier handling than the powder product, but has a disadvantage of poor shelf life compared to the powder product.

An example of a method of preparing in the form of beads is (a) 0.1-5.0 wt% solution of negatively charged biodegradable polymers such as alginate, pectin, carrageenan or polyaspartic acid. And a mixture of Bacillus cereus BC3 KCCM 10859P culture solution containing Ca ²⁺ , Cu ²⁺ , Zn ²⁺ , Ni ²⁺ , Co ²⁺ , Mn ²⁺ , Al ²⁺ , Fe ²⁺ , or Mg ²⁺ . Preparing a bead by dropping it in a metal salt solution at a concentration of 0.5-3.0 wt%; And

(b) the beads are immersed in a 0.1 to 3.0% by weight solution of a positively-charged biodegradable polymer such as chitosan, chitosan derivatives or polylysine and stirred for 30 minutes to 2 hours to coat the beads by electrostatic attraction. It can be prepared including the step. The microbial preparation prepared as described above may be used as it is by lyophilization, in order to increase the persistence of the survival and active effect of the microorganism; (c) washing the beads with 50 mM sodium acetate (pH 5.5) to remove excess positively charged biodegradable polymeric material; (d) immersing the beads in 0.1-2.0 wt% negative charge biodegradable polymer solution and stirring; And (e) further washing the beads with physiological saline and then immersing in 0.1 to 2.0 wt% positive charge biodegradable polymer solution to stir to form an outer membrane to form a thicker biodegradable polymer membrane thereon. It is possible to increase the effect persistence of the microbial agent produced by.

Another embodiment of the present invention is Bacillus megaterium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megaliumium BC2 -1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus cereus BC10 KCCM 10866P) and at least one Bacillus species selected from the group consisting of Bacillus cereus BC10 KCCM 10866P It provides a microbial agent for odor removal and heavy metal removal composed of Permus BC9 KCCM 10865P.

Another aspect of the invention is a method of culturing Bacillus permus BC9 KCCM 10865P with malodorous food waste or livestock wastewater to remove odors and heavy metals in food waste or livestock wastewater.

Another embodiment of the present invention Bacillus megaterium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megaliumium BC2 -1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus cereus BC10 KCCM 10866P (Bacillus cereus BC10 KCCM 10866P) one or more of the novel strain Bacillus species and an effective amount selected from the group consisting of Bacillus Mousse the malodor removing microbial preparations consisting BC9 KCCM 10865P odor is a method for culturing with that food waste, or livestock waste water to remove heavy metals in the odor and food waste, or livestock waste water.

Another embodiment of the present invention is an organic liquid fertilizer production method using livestock wastewater containing an effective amount of the new strain Bacillus permus BC9 KCCM 10865P and a livestock feed production method using food waste.

Organic liquid fertilizer production method using livestock wastewater and livestock feed production method using food waste according to the present invention can be applied to the conventional technology in addition to using the new strain Bacillus permus BC9 KCCM 10865P. Examples of organic liquid fertilizer production using livestock wastewater include a method for producing organic liquid fertilizer using livestock wastewater and peat. This method comprises the steps of pulverizing peat and organic material and oyster shells and briquettes at an appropriate ratio; Deodorizing by mixing a proper ratio of sodium bicarbonate, calcium oxide and calcium chloride; Fermentation using microorganisms; Extracting the organic liquid fertilizer; Neutralizing by adding dilute hydrochloric acid to the extracted organic liquid fertilizer; And aeration of the neutralized organic liquid for a predetermined time to reduce the chemical oxygen demand (C.O.D).

Fermentation feed using food waste using the new strain Bacillus permus BC9 KCCM 10865P, dry fermentation feed, mixed fermentation feed, mixing and dry fermentation combined method or liquid fermentation feed can be used. Dry fermentation feed method has a high weight reduction effect, but it is difficult to use in the state because the energy cost for drying is inconvenient and nutritionally unfinished state, and because it is high moisture (around 40%), packaging and automatic feeding The application of lines also has a disadvantage. The mixed fermentation feed method is capable of maintaining nutritional balance, but it is difficult to manufacture a variety of feeds having different requirements by age. The combined form of mixing and dry fermentation is easy to maintain nutritional balance and moisture control, but has a disadvantage of high cost, including the energy cost and moisture control agent input to dry. Liquid fermented feed produced at around 85% moisture content has a disadvantage in that it is difficult to distribute despite the advantage that the manufacturing cost is much cheaper than other methods.

Another embodiment of the present invention is Bacillus megaterium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megaliumium BC2 -1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), one or more Bacillus species selected from the group consisting of Bacillus cereus BC10 KCCM 10866P and an effective amount of the new strain Bacillus fur. Organic liquid fertilizer production method using livestock wastewater consisting of mousse BC9 KCCM 10865P and livestock feed production method using food waste.

DETAILED DESCRIPTION OF THE INVENTION

<Example 1> Bacillus permus BC9 ( Bacillus firmus  BC9) Isolation and Identification of Strains

Bacillus permus BC9 ( Bacillus firmus BC9) bacteria used in the present invention was obtained from mushrooms and mycelium masses inhabiting the leaves in the Samgimyeon forest, Iksan, Jeollabuk-do. The mushrooms and mycelium collected were put in a 500ml beaker, immersed in a culture solution prepared by adding 300ml of water and about 5g of soil sugar, and then incubated at 28-30 ° C.

After spraying a portion of the culture solution to the ceremonial toilet to see the odor disappears in one day, it was found that the Bacillus bacteria of the present invention has a good odor removal ability. The culture solution was continuously cultured using molasses, and subsequently used for food waste, it was also confirmed that the odor removal ability is excellent, and entered the pure separation of useful microorganisms.

Separation of useful microorganisms was performed at the Department of Microbiology, Kunsan National University, and a total of 12 microorganisms were isolated using NA medium. It confirmed that it was. The pure microorganisms were passaged and stored using NA medium. The 16S rDNA gene sequences of all 12 isolated Bacillus bacteria were analyzed by the Korea Institute of Biotechnology of Daejeon, Chungnam. As a result, Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), including Bacillus megaterium BC1-1 KCCM 10856P, Bacillus megaterium BC2- 1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P ( Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P ), Bacillus firmus BC9, Bacillus cereus BC10 KCCM 10866P ( Bacillus cereus BC10 KCCM 10866P) species. It was deposited with the Korean spawn center on April 27, 2007 and each KCCM accession number was assigned.

<Example 2> Bacillus permus BC9 ( Bacillus firmus  BC9) and Bacillus permus BC9 ( Bacillus firmus  Deodorization Effect of Livestock Wastewater by Mixed Microbial Composition of BC9) and Other Bacillus

Bacillus megaterium BC1-1 KCCM 10856P, Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-1 KCCM 10857P) Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megaterium BC2-1 KCCM 10858P ( Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P ( Bacillus cereus BC3 KCCM 10859P) (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P) Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus buffer mousse BC9 (Bacillus firmus BC9), Bacillus cereus BC10 KCCM 10866P (Bacill us cereus BC10 KCCM 10866P) The mixed microbial group was put into a microbial reaction vessel containing a fixed livestock wastewater (see FIG. 1 ), and after 4 days, the odor in the container was collected and measured.

The microbial reaction vessel condition of livestock wastewater was set up to maintain the temperature at 37.5 ℃ by using abandonment of constant flow rate and heating band and thermostat for the active activity of microorganism as follows. (See FIG. 1 ).

In addition, the concentration of odor generated in the livestock wastewater before microbial input was measured and analyzed for the change in concentration of each odor after microbial input. For accurate measurement of efficiency, two liters of wastewater were injected into each of 11 microbial reaction vessels to reduce the odor of livestock wastewater, and one vessel of 5 ml of Bacillus permus BC9 culture medium and the other vessel of Bacillus megalithium BC1-1 Bacillus megaterium BC1-1 KCCM 10856P, Bacillus megaterium BC1-2 KCCM 10857P, Bacillus megaterium BC1-2 KCCM 10857P, Bacillus megaterium BC2-1 KCCM 10858P, Bacillus megaterium BC2-1 KCCM 10858P Bacillus cereus BC3 KCCM 10859P ( Bacillus cereus BC3 KCCM 10859P), Bacillus rickeniformis BC4 KCCM 10860P ( Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P ( Bacillus cereus BC5 KCCM 10861P) 10862P ( Bacillus sphaericus BC6 KCCM 10862P), Bacillus Clauci BC7 KCCM 10863P ( Bacillus clausii BC7 KCCM 10863P), Bacillus rickeniformis BC8 KCCM 10864P ( Bacillus licheniformis BC8 KC CM 10864P), Bacillus firmus BC9 ( Bacillus firmus BC9), Bacillus cereus BC10 KCCM 10866P Was used as a control wastewater.

(1) analysis method

1) Measurement method of ammonia

Ammonia is quantified by measuring the absorbance of indophenols produced by adding phenol-nitroprusside sodium solution and sodium hypochlorite solution to the analytical sample solution and reacting with ammonium ion.

end. reagent

-Collecting solution

5 g of boric acid is dissolved in distilled water, and the total amount is 1 L.

-Phenol, pentacyano nitrosyl sodium (III) acid solution

5 g of phenol and 25 mg of pentacyano nitrosyl ammonium iron (III) sodium dihydrate6) are dissolved in distilled water to make a total amount of 500 mL. Store this solution in a cool dark place and do not use more than 1 month after preparation.

Sodium hypochlorite solution

Sodium hypochlorite solution (3-10% effective chlorine) 60 / CmL [where C is the effective chlorine concentration (in%) of sodium hypochlorite (NaOCl) quantified at the time of preparation)], 10 g of sodium hydroxide and sodium hydrogen phosphate 12 Dissolve 35.8 g of hydrate in distilled water to make the total amount 1 L. This solution is prepared each time it is used.

I. Solution absorption

The absorber bottle is made of hard glass that can hold 20 mL of absorbent liquid and is equipped with a filter port. In the middle, take a sample solution that can hold 20 mL of absorbent liquid and connect the two in series. The suction pump draws more than 10 L / min of air with an absorbent bottle installed. After collection, the absorbent solutions from the two absorbent bottles are combined and transferred to a 50 mL volumetric flask. The absorbed solution is then transferred to the volumetric flask and the total volume is 50 mL. Transfer 10 mL of this solution into the test tube and use it as the sample solution for analysis.

All. Absorbance Measurement of Analytical Sample Solution

Add 5 mL of phenol pentacyano nitrosyl iron (III) solution to the sample solution for analysis, shake well, mix 5 mL of sodium hypochlorite solution, and leave at 25 to 30 ° C for 1 hour. Absorbance is measured at. For blank test solution, use 10 mL of the collecting solution (absorbent solution) in the same manner as the analytical sample solution.

2) Methylmercaptan, hydrogen sulfide, dimethyl sulfide and dimethyl disulfide test method

In the sulfide method, the sample gas was concentrated at -170 ° C. in a sample preconcentration trap (SPT) at low temperature, heated again, desorbed, and injected into a gas chromatograph, and a PFPD (Pulse Flame Photometri Dectector) was used as a detector.

3) trimethylamine

Trimethylamine was analyzed by diluting the sample solution for analysis absorbed in aqueous sulfuric acid solution (359 + 1) with a syringe, and injecting it through a silicone stopper into a decomposition bottle containing potassium hydroxide solution. The trimethylamine generated by bubbling about 2-3 L of nitrogen was cooled with a liquid acid, concentrated in a condenser tube, heated and desorbed to about 70 ° C., and trimethylamine was introduced into a column and analyzed by FID.

4) Analysis of Aldehydes

Analysis of aldehydes is carried out through a sample collection tube filled with octadecyl silylated silica gel coated with 2,4-dinitrophenyl hydrazine, to collect aldehydes and to evaporate acetonitrile from the sample collection tube. The mixture was dissolved in ethyl acetate, and a portion thereof was introduced into HPLC (Young Lin) for quantification.

(2) Analysis result

Bacillus permus BC9 and Bacillus permus BC9 mixed with the microorganism composition of other Bacillus bacteria and the result of the four days culture and only the control waste water is shown in Table 1 below.

TABLE 1

Bacillus permus BC9 had high removal of ammonia, hydrogen sulfide, methyl mercaptan, dimethyl disulfide and trimethylamine from the livestock wastewater. Dimethyl sulfide and acetaldehyde were detected at almost the same concentration as before control, 4 days after the control sample and mixed microbial input sample, and the removal efficiency for dimethyl sulfide and acetaldehyde seems to be insignificant.

<Example 3> Odor analysis result of food waste by mixed microorganism composition of Bacillus permus BC9 and Bacillus permus BC9 and other Bacillus bacteria

Bacillus permutus BC9 and Bacillus megalium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megalium BC1-2 KCCM 10857P Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megaterium BC2-1 KCCM 10858P ( Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P ( Bacillus cereus BC3 KCCM 10859P) (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P) Bacillus licheniformis BC8 KCCM 10864P, Bacillus firmus BC9, Bacillus cereus BC10 KCCM 10866P Bacillus cereus BC10 KCCM 10866P) The mixed microbial group was put into a microbial reaction vessel containing a certain food waste (see FIG. 2 ), and after 4 days, the odor in the container was collected and measured.

Microbial reaction vessel conditions of food waste was viscous to maintain a constant temperature to 37.5 ℃ using a heating band (heating band) and a thermostat (see Figure 2 ).

In addition, the concentration of odor generated by raw food waste was measured before microbial input, and the concentration change of each odor after microbial input was analyzed. In order to accurately measure the odor of food waste, two liters of 11 microbial reaction vessels were injected into each of the microbial reaction vessels, and one vessel of 5 ml of Bacillus permus BC9 culture medium and the other vessel of Bacillus megacium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megaterium BC2-1 KCCM 10858P ( Bacillus megaterium BC2-1 KCCM 10858P), Bacillus serenity mouse BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P), Bacillus spa Erie kusu BC6 KCCM 10862P ( Bacillus sphaericus BC6 KCCM 10862P), Bacillus Klaus BC7 KCCM 10863P ( Bacillus clausii BC7 KCCM 10863P), Bacillus rickeniformis BC8 KCCM 10864P ( Bacillus licheniformis B C8 KCCM 10864P), Bacillus firmus BC9, Bacillus cereus BC10 KCCM 10866P 5 ml of waste was added and used as a control.

Odor analysis of food waste was carried out in the same manner as the livestock wastewater odor analysis experimental method of Example 2.

<Result>

Table 2

Bacillus permus BC9 removes ammonia, hydrogen sulfide, methyl mercaptan, dimethyl disulfide and trimethylamine from food waste. However, dimethyl sulfide and acetaldehyde were detected at almost the same concentration as the control sample and the mixed microbial input sample after 4 days after the addition, and the removal efficiency of dimethyl sulfide and acetaldehyde seems to be insignificant.

<Example 4> Heavy Metal Reduction Effect of Livestock Wastewater and Food Waste

In order to measure the effect of reducing heavy metals in livestock wastewater and food waste by Bacillus permus BC9, 2 liters of food waste were injected into each of 11 microbial reaction vessels, and then one vessel was 5 ml of Bacillus permus BC9 culture solution and another vessel. Bacillus megaterium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megaterium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megalium BC2-1 KCCM 10858P ( Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P ), Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Basil Srei Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus buffer mousse BC9 (Bacillus firmus BC9), Bacillus cereus BC10 KCCM 10866P (Bacillus cereus BC10 KCCM 10866P) culture solution is evenly mixed mixture microorganism 5ml input, One container was used as a control by putting 5ml of food waste without adding microorganisms. The same was done for livestock wastewater. The concentration of heavy metals in food waste and livestock wastewater was measured during microbial culture and after 4 days of culture. Heavy metal was measured by the following method.

(1) Preparation of Reagent

Ternary solution: Mix HNO ₃ , H ₂ SO ₄ and HClO ₄ in the ratio of 10: 1: 4 to prepare the required amount.

-Concentrated HNO ₃

(2) disassembly

10 ml of the collected sample is weighed into a 250 ml Erlenmeyer flask, 10-30 ml of concentrated HNO _{3 is} added, and left to stand overnight, then placed on a hot plate and dried slowly until a white precipitate is formed. After cooling, 10-50 ml of ternary solution is added and heated on a hot plate to blow off some white smoke of H ₂ SO ₄ and HClO ₄ , and the decomposition stops when the decomposition solution becomes white or brown. After cooling, hot distilled water is added and filtered using a No. 6 filter paper to a 50 or 100 ml mass flask, followed by rinsing the digestion flask with hot distilled water and thoroughly filtered using a policymen.

(3) measurement

The filtrate is diluted in a predetermined amount, and then K, Ca, Mg, Na, Fe, Mn, Cu, Zn, Cr, Cd, Pb, Ni, and the like are collected in AA (Atomic Absorption Flame Emission Spectrometer) or ICP (Inductively Coupled Plasma). Make a calibration curve with the standard solution of each component and measure it.

TABLE 3

Table 4

Bacillus permus BC9 removed cadmium, chromium, copper, zinc and lead from livestock wastewater. In addition, cadmium, copper, and zinc were removed from food waste. However, the amount of chromium and lead was increased in food waste, showing different results from the livestock wastewater, and the effect of removing chromium and lead by Bacillus permus BC9 was uncertain.

Bacillus permus BC9 KCCM 10865P according to the present invention was excellent in removing odor from livestock wastewater and food waste, and it takes about 30 days to remove the odor of the conventional microbial mixture (hereinafter referred to as EM) Compared to the point (without data), the odor is removed at a very high speed, and the odor removal ability is excellent. At the same time, the strains of the present invention also have the ability to remove heavy metals at very high levels for some of the heavy metals contained in livestock wastewater or food waste.

Claims (9)

Bacillus firmus BC9 Bacillus firmus BC9 useful for removing odors and heavy metals. KCCM 10865P. New strain Bacillus permus BC9 KCCM 10865P containing microorganisms for odor removal and heavy metal removal. Bacillus megaterium BC1-1 KCCM 10856P ( Bacillus megaterium BC1-1 KCCM 10856P), Bacillus megacerium BC1-2 KCCM 10857P ( Bacillus megaterium BC1-2 KCCM 10857P), Bacillus megalium BC2-1 KCCM 10858P ( Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P) Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus cereus BC10 Consisting of one or more Bacillus species selected from the group consisting of KCCM 10866P ( Bacillus cereus BC10 KCCM 10866P) and an effective amount of the new strain Bacillus permus BC9 KCCM 10865P Microbial preparation for odor removal and heavy metal removal. New strain Bacillus permus BC9 KCCM 10865P is incubated with odorous food waste or livestock wastewater to remove odors and heavy metals in food waste or livestock wastewater. Bacillus mega site Solarium BC1-1 KCCM 10856P (Bacillus megaterium BC1-1 KCCM 10856P), Bacillus mega site Solarium BC1-2 KCCM 10857P (Bacillus megaterium BC1-2 KCCM 10857P), Bacillus mega site Solarium BC2-1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P) Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus cereus BC10 Consisting of one or more Bacillus species selected from the group consisting of KCCM 10866P ( Bacillus cereus BC10 KCCM 10866P) and an effective amount of the new strain Bacillus permus BC9 KCCM 10865P A method for removing odors and heavy metals in food waste or livestock waste by incubating the odor removing microorganism with odorous food waste or livestock wastewater. A method for producing organic liquor using livestock wastewater containing an effective amount of the new strain Bacillus permus BC9 KCCM 10865P. Bacillus mega site Solarium BC1-1 KCCM 10856P (Bacillus megaterium BC1-1 KCCM 10856P), Bacillus mega site Solarium BC1-2 KCCM 10857P (Bacillus megaterium BC1-2 KCCM 10857P), Bacillus mega site Solarium BC2-1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P) Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus cereus BC10 Consisting of one or more Bacillus species selected from the group consisting of KCCM 10866P ( Bacillus cereus BC10 KCCM 10866P) and an effective amount of the new strain Bacillus permus BC9 KCCM 10865P Organic liquid fertilizer production method using livestock wastewater. A method for producing livestock feed using food waste containing an effective amount of new strain Bacillus permus BC9 KCCM 10865P. Bacillus mega site Solarium BC1-1 KCCM 10856P (Bacillus megaterium BC1-1 KCCM 10856P), Bacillus mega site Solarium BC1-2 KCCM 10857P (Bacillus megaterium BC1-2 KCCM 10857P), Bacillus mega site Solarium BC2-1 KCCM 10858P (Bacillus megaterium BC2-1 KCCM 10858P), Bacillus cereus BC3 KCCM 10859P (Bacillus cereus BC3 KCCM 10859P), Bacillus Lai Kenny Po Ms BC4 KCCM 10860P (Bacillus licheniformis BC4 KCCM 10860P), Bacillus cereus BC5 KCCM 10861P (Bacillus cereus BC5 KCCM 10861P) Bacillus spa Erie kusu BC6 KCCM 10862P (Bacillus sphaericus BC6 KCCM 10862P), Bacillus claw when BC7 KCCM 10863P (Bacillus clausii BC7 KCCM 10863P), Bacillus Lai Kenny Po Ms BC8 KCCM 10864P (Bacillus licheniformis BC8 KCCM 10864P), Bacillus cereus BC10 Consists of at least one Bacillus species selected from the group consisting of KCCM 10866P ( Bacillus cereus BC10 KCCM 10866P) and an effective amount of the new strain Bacillus permus BC9 KCCM 10865P Method of producing livestock feed using processed food waste.

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